                         SEQUENCE LISTING

<110>  Athenix Corp.
       Thayer, Rebecca
       Sampson, Kimberly
       Lehtinen, Duane
       Peters, Cheryl
 
<120>  AXMI281 TOXIN GENE AND METHODS FOR ITS USE

<130>  APA13-6049

<150>  61/863,966
<151>  2013-08-09

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1035
<212>  DNA
<213>  Bacillus thuringiensis

<400>  1
atgctaatag gaaatggaat gatttttcca aacgagagaa gattatttta tatgatgaaa       60

aaaaataata acgaaaaata tggaccaaat ggaatagtta acgaaaaaga atttattctc      120

ttcacagaac aatggatatc tttacaaaca tatgccatca caggaactag tttacctata      180

aatgaggttg aaatgcgtaa acatattggg ttatcacaaa atacaaaatc accacaatgc      240

tttaaaaatt tgtatgatat tcattaccaa aataaacatc tatgtacctg gtggaataat      300

gacttagttc cattaatact taaaaccatc cataatattt ccgcttacgg atttaaatgc      360

gctggaaagg gtactactac aggatattat aaagccctac aagctgattt agaaactatt      420

tcacttaatt ccaattcact aacacaagaa aaagcattta aagattttaa aaaacgttgt      480

caacttcttc ataaagaagc cttacaatat aagactacag cagaaaatgc tgagaaatgg      540

ttaaatatat tttttgaagg tggaaaagac tcaaatgata atgatgtaat tggagtagca      600

gctattcaaa cacattttaa aaaaataaat gctcatcttg ataacaaata tcaaaatagt      660

aactcaaaat ataaaggatt actatcaaat atgaaccaac taaaacaagg tttagtcaac      720

aacataacag gaaaacagaa tttttctaat aaaaaagtta aaaaaatgat aattgaaatg      780

atcgataaaa ttaacacaca aggtgaaaat atactaaaat caaaacccca aatcatgcta      840

atatttcaaa aaattagtga atcttggtct attataagaa attatatgag taatcttgcg      900

aatgcatctg taaacaagtt gcagaaagaa tctgacaaaa tatatcttga acttaagaat      960

agcaccaatt cctggctaga aatagctaca caaacaagaa actttgcact aaatctttat     1020

aatctaaaaa ctcca                                                      1035


<210>  2
<211>  345
<212>  PRT
<213>  Bacillus thuringiensis

<400>  2

Met Leu Ile Gly Asn Gly Met Ile Phe Pro Asn Glu Arg Arg Leu Phe 
1               5                   10                  15      


Tyr Met Met Lys Lys Asn Asn Asn Glu Lys Tyr Gly Pro Asn Gly Ile 
            20                  25                  30          


Val Asn Glu Lys Glu Phe Ile Leu Phe Thr Glu Gln Trp Ile Ser Leu 
        35                  40                  45              


Gln Thr Tyr Ala Ile Thr Gly Thr Ser Leu Pro Ile Asn Glu Val Glu 
    50                  55                  60                  


Met Arg Lys His Ile Gly Leu Ser Gln Asn Thr Lys Ser Pro Gln Cys 
65                  70                  75                  80  


Phe Lys Asn Leu Tyr Asp Ile His Tyr Gln Asn Lys His Leu Cys Thr 
                85                  90                  95      


Trp Trp Asn Asn Asp Leu Val Pro Leu Ile Leu Lys Thr Ile His Asn 
            100                 105                 110         


Ile Ser Ala Tyr Gly Phe Lys Cys Ala Gly Lys Gly Thr Thr Thr Gly 
        115                 120                 125             


Tyr Tyr Lys Ala Leu Gln Ala Asp Leu Glu Thr Ile Ser Leu Asn Ser 
    130                 135                 140                 


Asn Ser Leu Thr Gln Glu Lys Ala Phe Lys Asp Phe Lys Lys Arg Cys 
145                 150                 155                 160 


Gln Leu Leu His Lys Glu Ala Leu Gln Tyr Lys Thr Thr Ala Glu Asn 
                165                 170                 175     


Ala Glu Lys Trp Leu Asn Ile Phe Phe Glu Gly Gly Lys Asp Ser Asn 
            180                 185                 190         


Asp Asn Asp Val Ile Gly Val Ala Ala Ile Gln Thr His Phe Lys Lys 
        195                 200                 205             


Ile Asn Ala His Leu Asp Asn Lys Tyr Gln Asn Ser Asn Ser Lys Tyr 
    210                 215                 220                 


Lys Gly Leu Leu Ser Asn Met Asn Gln Leu Lys Gln Gly Leu Val Asn 
225                 230                 235                 240 


Asn Ile Thr Gly Lys Gln Asn Phe Ser Asn Lys Lys Val Lys Lys Met 
                245                 250                 255     


Ile Ile Glu Met Ile Asp Lys Ile Asn Thr Gln Gly Glu Asn Ile Leu 
            260                 265                 270         


Lys Ser Lys Pro Gln Ile Met Leu Ile Phe Gln Lys Ile Ser Glu Ser 
        275                 280                 285             


Trp Ser Ile Ile Arg Asn Tyr Met Ser Asn Leu Ala Asn Ala Ser Val 
    290                 295                 300                 


Asn Lys Leu Gln Lys Glu Ser Asp Lys Ile Tyr Leu Glu Leu Lys Asn 
305                 310                 315                 320 


Ser Thr Asn Ser Trp Leu Glu Ile Ala Thr Gln Thr Arg Asn Phe Ala 
                325                 330                 335     


Leu Asn Leu Tyr Asn Leu Lys Thr Pro 
            340                 345 


<210>  3
<211>  339
<212>  PRT
<213>  Bacillus thuringiensis

<400>  3

Met Ile Phe Pro Asn Glu Arg Arg Leu Phe Tyr Met Met Lys Lys Asn 
1               5                   10                  15      


Asn Asn Glu Lys Tyr Gly Pro Asn Gly Ile Val Asn Glu Lys Glu Phe 
            20                  25                  30          


Ile Leu Phe Thr Glu Gln Trp Ile Ser Leu Gln Thr Tyr Ala Ile Thr 
        35                  40                  45              


Gly Thr Ser Leu Pro Ile Asn Glu Val Glu Met Arg Lys His Ile Gly 
    50                  55                  60                  


Leu Ser Gln Asn Thr Lys Ser Pro Gln Cys Phe Lys Asn Leu Tyr Asp 
65                  70                  75                  80  


Ile His Tyr Gln Asn Lys His Leu Cys Thr Trp Trp Asn Asn Asp Leu 
                85                  90                  95      


Val Pro Leu Ile Leu Lys Thr Ile His Asn Ile Ser Ala Tyr Gly Phe 
            100                 105                 110         


Lys Cys Ala Gly Lys Gly Thr Thr Thr Gly Tyr Tyr Lys Ala Leu Gln 
        115                 120                 125             


Ala Asp Leu Glu Thr Ile Ser Leu Asn Ser Asn Ser Leu Thr Gln Glu 
    130                 135                 140                 


Lys Ala Phe Lys Asp Phe Lys Lys Arg Cys Gln Leu Leu His Lys Glu 
145                 150                 155                 160 


Ala Leu Gln Tyr Lys Thr Thr Ala Glu Asn Ala Glu Lys Trp Leu Asn 
                165                 170                 175     


Ile Phe Phe Glu Gly Gly Lys Asp Ser Asn Asp Asn Asp Val Ile Gly 
            180                 185                 190         


Val Ala Ala Ile Gln Thr His Phe Lys Lys Ile Asn Ala His Leu Asp 
        195                 200                 205             


Asn Lys Tyr Gln Asn Ser Asn Ser Lys Tyr Lys Gly Leu Leu Ser Asn 
    210                 215                 220                 


Met Asn Gln Leu Lys Gln Gly Leu Val Asn Asn Ile Thr Gly Lys Gln 
225                 230                 235                 240 


Asn Phe Ser Asn Lys Lys Val Lys Lys Met Ile Ile Glu Met Ile Asp 
                245                 250                 255     


Lys Ile Asn Thr Gln Gly Glu Asn Ile Leu Lys Ser Lys Pro Gln Ile 
            260                 265                 270         


Met Leu Ile Phe Gln Lys Ile Ser Glu Ser Trp Ser Ile Ile Arg Asn 
        275                 280                 285             


Tyr Met Ser Asn Leu Ala Asn Ala Ser Val Asn Lys Leu Gln Lys Glu 
    290                 295                 300                 


Ser Asp Lys Ile Tyr Leu Glu Leu Lys Asn Ser Thr Asn Ser Trp Leu 
305                 310                 315                 320 


Glu Ile Ala Thr Gln Thr Arg Asn Phe Ala Leu Asn Leu Tyr Asn Leu 
                325                 330                 335     


Lys Thr Pro 
            


<210>  4
<211>  328
<212>  PRT
<213>  Bacillus thuringiensis

<400>  4

Met Met Lys Lys Asn Asn Asn Glu Lys Tyr Gly Pro Asn Gly Ile Val 
1               5                   10                  15      


Asn Glu Lys Glu Phe Ile Leu Phe Thr Glu Gln Trp Ile Ser Leu Gln 
            20                  25                  30          


Thr Tyr Ala Ile Thr Gly Thr Ser Leu Pro Ile Asn Glu Val Glu Met 
        35                  40                  45              


Arg Lys His Ile Gly Leu Ser Gln Asn Thr Lys Ser Pro Gln Cys Phe 
    50                  55                  60                  


Lys Asn Leu Tyr Asp Ile His Tyr Gln Asn Lys His Leu Cys Thr Trp 
65                  70                  75                  80  


Trp Asn Asn Asp Leu Val Pro Leu Ile Leu Lys Thr Ile His Asn Ile 
                85                  90                  95      


Ser Ala Tyr Gly Phe Lys Cys Ala Gly Lys Gly Thr Thr Thr Gly Tyr 
            100                 105                 110         


Tyr Lys Ala Leu Gln Ala Asp Leu Glu Thr Ile Ser Leu Asn Ser Asn 
        115                 120                 125             


Ser Leu Thr Gln Glu Lys Ala Phe Lys Asp Phe Lys Lys Arg Cys Gln 
    130                 135                 140                 


Leu Leu His Lys Glu Ala Leu Gln Tyr Lys Thr Thr Ala Glu Asn Ala 
145                 150                 155                 160 


Glu Lys Trp Leu Asn Ile Phe Phe Glu Gly Gly Lys Asp Ser Asn Asp 
                165                 170                 175     


Asn Asp Val Ile Gly Val Ala Ala Ile Gln Thr His Phe Lys Lys Ile 
            180                 185                 190         


Asn Ala His Leu Asp Asn Lys Tyr Gln Asn Ser Asn Ser Lys Tyr Lys 
        195                 200                 205             


Gly Leu Leu Ser Asn Met Asn Gln Leu Lys Gln Gly Leu Val Asn Asn 
    210                 215                 220                 


Ile Thr Gly Lys Gln Asn Phe Ser Asn Lys Lys Val Lys Lys Met Ile 
225                 230                 235                 240 


Ile Glu Met Ile Asp Lys Ile Asn Thr Gln Gly Glu Asn Ile Leu Lys 
                245                 250                 255     


Ser Lys Pro Gln Ile Met Leu Ile Phe Gln Lys Ile Ser Glu Ser Trp 
            260                 265                 270         


Ser Ile Ile Arg Asn Tyr Met Ser Asn Leu Ala Asn Ala Ser Val Asn 
        275                 280                 285             


Lys Leu Gln Lys Glu Ser Asp Lys Ile Tyr Leu Glu Leu Lys Asn Ser 
    290                 295                 300                 


Thr Asn Ser Trp Leu Glu Ile Ala Thr Gln Thr Arg Asn Phe Ala Leu 
305                 310                 315                 320 


Asn Leu Tyr Asn Leu Lys Thr Pro 
                325             


<210>  5
<211>  327
<212>  PRT
<213>  Bacillus thuringiensis

<400>  5

Met Lys Lys Asn Asn Asn Glu Lys Tyr Gly Pro Asn Gly Ile Val Asn 
1               5                   10                  15      


Glu Lys Glu Phe Ile Leu Phe Thr Glu Gln Trp Ile Ser Leu Gln Thr 
            20                  25                  30          


Tyr Ala Ile Thr Gly Thr Ser Leu Pro Ile Asn Glu Val Glu Met Arg 
        35                  40                  45              


Lys His Ile Gly Leu Ser Gln Asn Thr Lys Ser Pro Gln Cys Phe Lys 
    50                  55                  60                  


Asn Leu Tyr Asp Ile His Tyr Gln Asn Lys His Leu Cys Thr Trp Trp 
65                  70                  75                  80  


Asn Asn Asp Leu Val Pro Leu Ile Leu Lys Thr Ile His Asn Ile Ser 
                85                  90                  95      


Ala Tyr Gly Phe Lys Cys Ala Gly Lys Gly Thr Thr Thr Gly Tyr Tyr 
            100                 105                 110         


Lys Ala Leu Gln Ala Asp Leu Glu Thr Ile Ser Leu Asn Ser Asn Ser 
        115                 120                 125             


Leu Thr Gln Glu Lys Ala Phe Lys Asp Phe Lys Lys Arg Cys Gln Leu 
    130                 135                 140                 


Leu His Lys Glu Ala Leu Gln Tyr Lys Thr Thr Ala Glu Asn Ala Glu 
145                 150                 155                 160 


Lys Trp Leu Asn Ile Phe Phe Glu Gly Gly Lys Asp Ser Asn Asp Asn 
                165                 170                 175     


Asp Val Ile Gly Val Ala Ala Ile Gln Thr His Phe Lys Lys Ile Asn 
            180                 185                 190         


Ala His Leu Asp Asn Lys Tyr Gln Asn Ser Asn Ser Lys Tyr Lys Gly 
        195                 200                 205             


Leu Leu Ser Asn Met Asn Gln Leu Lys Gln Gly Leu Val Asn Asn Ile 
    210                 215                 220                 


Thr Gly Lys Gln Asn Phe Ser Asn Lys Lys Val Lys Lys Met Ile Ile 
225                 230                 235                 240 


Glu Met Ile Asp Lys Ile Asn Thr Gln Gly Glu Asn Ile Leu Lys Ser 
                245                 250                 255     


Lys Pro Gln Ile Met Leu Ile Phe Gln Lys Ile Ser Glu Ser Trp Ser 
            260                 265                 270         


Ile Ile Arg Asn Tyr Met Ser Asn Leu Ala Asn Ala Ser Val Asn Lys 
        275                 280                 285             


Leu Gln Lys Glu Ser Asp Lys Ile Tyr Leu Glu Leu Lys Asn Ser Thr 
    290                 295                 300                 


Asn Ser Trp Leu Glu Ile Ala Thr Gln Thr Arg Asn Phe Ala Leu Asn 
305                 310                 315                 320 


Leu Tyr Asn Leu Lys Thr Pro 
                325         


<210>  6
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  endoplasmic reticulum targeting peptide

<400>  6

Lys Asp Glu Leu 
1               


