                                SEQUENCE LISTING

<110> Sun, Lan
      Ferrell, Ngim Muy Ung
      Singh, Deepak
      Headman, Jennifer
      Chu, Mei-Hua
      Guerini, Michael
      Jacobson, Sandra

<120> Improved Beta-Glucosidase Enzymes for
  Increased Biomass Saccharification
  

<130> 91997-919358

<140> WO Not yet assigned 
<141> Not yet assigned  

<150> US 61/878,989 
<151> 2013-09-17  

<160> 36

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2580
<212> DNA
<213> Aspergillus niger

<220> 
<223> wild type beta-glucosidase (BG)

<400> 1
atgagattta ctttgattga agctgttgct ttgactgctg tttctttggc ttctgctgat 60
gaattggctt attctccacc atattatcca tctccatggg ctaatggtca aggtgattgg 120
gctcaagctt atcaaagagc tgttgatatt gtttctcaaa tgactttgga tgaaaaagtt 180
aatttgacta ctggtactgg ttgggaattg gaattgtgtg ttggtcaaac tggtggtgtt 240
ccaagattgg gtgttccagg tatgtgtttg caagattctc cattgggtgt tagagattct 300
gattataatt ctgcttttcc agctggtatg aatgttgctg ctacttggga taaaaatttg 360
gcttatttga gaggtaaagc tatgggtcaa gaattttctg ataaaggtgc tgatattcaa 420
ttgggtccag ctgctggtcc attgggtaga tctccagatg gtggtagaaa ttgggaaggt 480
ttttctccag atccagcttt gtctggtgtt ttgtttgctg aaactattaa aggtattcaa 540
gatgctggtg ttgttgctac tgctaaacat tatattgctt atgaacaaga acattttaga 600
caagctccag aagctcaagg ttttggtttt aatatttctg aatctggttc tgctaatttg 660
gatgataaaa ctatgcatga attgtatttg tggccatttg ctgatgctat tagagctggt 720
gctggtgctg ttatgtgttc ttataatcaa attaataatt cttatggttg tcaaaattct 780
tatactttga ataaattgtt gaaagctgaa ttgggttttc aaggttttgt tatgtctgat 840
tgggctgctc atcatgctgg tgtttctggt gctttggctg gtttggatat gtctatgcca 900
ggtgatgttg attatgattc tggtacttct tattggggta ctaatttgac tatttctgtt 960
ttgaatggta ctgttccaca atggagagtt gatgatatgg ctgttagaat tatggctgct 1020
tattataaag ttggtagaga tagattgtgg actccaccaa atttttcttc ttggactaga 1080
gatgaatatg gttataaata ttattatgtt tctgaaggtc catatgaaaa agttaatcaa 1140
tatgttaatg ttcaaagaaa tcattctgaa ttgattagaa gaattggtgc tgattctact 1200
gttttgttga aaaatgatgg tgctttgcca ttgactggta aagaaagatt ggttgctttg 1260
attggtgaag atgctggttc taatccatat ggtgctaatg gttgttctga tagaggttgt 1320
gataatggta ctttggctat gggttggggt tctggtactg ctaattttcc atatttggtt 1380
actccagaac aagctatttc taatgaagtt ttgaaacata aaaatggtgt ttttactgct 1440
actgataatt gggctattga tcaaattgaa gctttggcta aaactgcttc tgtttctttg 1500
gtttttgtta atgctgattc tggtgaaggt tatattaatg ttgatggtaa tttgggtgat 1560
agaagaaatt tgactttgtg gagaaatggt gataatgtta ttaaagctgc tgcttctaat 1620
tgtaataata ctattgttgt tattcattct gttggtccag ttttggttaa tgaatggtat 1680
gataatccaa atgttactgc tattttgtgg ggtggtttgc caggtcaaga atctggtaat 1740
tctttggctg atgttttgta tggtagagtt aatccaggtg ctaaatctcc atttacttgg 1800
ggtaaaacta gagaagctta tcaagattat ttggttactg aaccaaataa tggtaatggt 1860
gctccacaag aagattttgt tgaaggtgtt tttattgatt atagaggttt tgataaaaga 1920
aatgaaactc caatttatga atttggttat ggtttgtctt atactacttt taattattct 1980
aatttggaag ttcaagtttt gtctgctcca gcttatgaac cagcttctgg tgaaactgaa 2040
gctgctccaa cttttggtga agttggtaat gcttctgatt atttgtatcc atctggtttg 2100
caaagaatta ctaaatttat ttatccatgg ttgaatggta ctgatttgga agcttcttct 2160
ggtgatgctt cttatggtca agattcttct gattatttgc cagaaggtgc tactgatggt 2220
tctgctcaac caattttgcc agctggtggt ggtccaggtg gtaatccaag attgtatgat 2280
gaattgatta gagtttctgt tactattaaa aatactggta aagttgctgg tgatgaagtt 2340
ccacaattgt atgtttcttt gggtggtcca aatgaaccaa aaattgtttt gagacaattt 2400
gaaagaatta ctttgcaacc atctgaagaa actaaatggt ctactacttt gactagaaga 2460
gatttggcta attggaatgt tgaaaaacaa gattgggaaa ttacttctta tccaaaaatg 2520
gtttttgttg gttcttcttc tagaaaattg ccattgagag cttctttgcc aactgttcat 2580


<210> 2
<211> 860
<212> PRT
<213> Aspergillus niger

<220> 
<223> wild type beta-glucosidase (BG)

<400> 2
Met Arg Phe Thr Leu Ile Glu Ala Val Ala Leu Thr Ala Val Ser Leu
 1               5                  10                  15      
Ala Ser Ala Asp Glu Leu Ala Tyr Ser Pro Pro Tyr Tyr Pro Ser Pro
            20                  25                  30          
Trp Ala Asn Gly Gln Gly Asp Trp Ala Gln Ala Tyr Gln Arg Ala Val
        35                  40                  45              
Asp Ile Val Ser Gln Met Thr Leu Asp Glu Lys Val Asn Leu Thr Thr
    50                  55                  60                  
Gly Thr Gly Trp Glu Leu Glu Leu Cys Val Gly Gln Thr Gly Gly Val
65                  70                  75                  80  
Pro Arg Leu Gly Val Pro Gly Met Cys Leu Gln Asp Ser Pro Leu Gly
                85                  90                  95      
Val Arg Asp Ser Asp Tyr Asn Ser Ala Phe Pro Ala Gly Met Asn Val
            100                 105                 110         
Ala Ala Thr Trp Asp Lys Asn Leu Ala Tyr Leu Arg Gly Lys Ala Met
        115                 120                 125             
Gly Gln Glu Phe Ser Asp Lys Gly Ala Asp Ile Gln Leu Gly Pro Ala
    130                 135                 140                 
Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn Trp Glu Gly
145                 150                 155                 160 
Phe Ser Pro Asp Pro Ala Leu Ser Gly Val Leu Phe Ala Glu Thr Ile
                165                 170                 175     
Lys Gly Ile Gln Asp Ala Gly Val Val Ala Thr Ala Lys His Tyr Ile
            180                 185                 190         
Ala Tyr Glu Gln Glu His Phe Arg Gln Ala Pro Glu Ala Gln Gly Phe
        195                 200                 205             
Gly Phe Asn Ile Ser Glu Ser Gly Ser Ala Asn Leu Asp Asp Lys Thr
    210                 215                 220                 
Met His Glu Leu Tyr Leu Trp Pro Phe Ala Asp Ala Ile Arg Ala Gly
225                 230                 235                 240 
Ala Gly Ala Val Met Cys Ser Tyr Asn Gln Ile Asn Asn Ser Tyr Gly
                245                 250                 255     
Cys Gln Asn Ser Tyr Thr Leu Asn Lys Leu Leu Lys Ala Glu Leu Gly
            260                 265                 270         
Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Ala Gly Val
        275                 280                 285             
Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp Val Asp
    290                 295                 300                 
Tyr Asp Ser Gly Thr Ser Tyr Trp Gly Thr Asn Leu Thr Ile Ser Val
305                 310                 315                 320 
Leu Asn Gly Thr Val Pro Gln Trp Arg Val Asp Asp Met Ala Val Arg
                325                 330                 335     
Ile Met Ala Ala Tyr Tyr Lys Val Gly Arg Asp Arg Leu Trp Thr Pro
            340                 345                 350         
Pro Asn Phe Ser Ser Trp Thr Arg Asp Glu Tyr Gly Tyr Lys Tyr Tyr
        355                 360                 365             
Tyr Val Ser Glu Gly Pro Tyr Glu Lys Val Asn Gln Tyr Val Asn Val
    370                 375                 380                 
Gln Arg Asn His Ser Glu Leu Ile Arg Arg Ile Gly Ala Asp Ser Thr
385                 390                 395                 400 
Val Leu Leu Lys Asn Asp Gly Ala Leu Pro Leu Thr Gly Lys Glu Arg
                405                 410                 415     
Leu Val Ala Leu Ile Gly Glu Asp Ala Gly Ser Asn Pro Tyr Gly Ala
            420                 425                 430         
Asn Gly Cys Ser Asp Arg Gly Cys Asp Asn Gly Thr Leu Ala Met Gly
        435                 440                 445             
Trp Gly Ser Gly Thr Ala Asn Phe Pro Tyr Leu Val Thr Pro Glu Gln
    450                 455                 460                 
Ala Ile Ser Asn Glu Val Leu Lys His Lys Asn Gly Val Phe Thr Ala
465                 470                 475                 480 
Thr Asp Asn Trp Ala Ile Asp Gln Ile Glu Ala Leu Ala Lys Thr Ala
                485                 490                 495     
Ser Val Ser Leu Val Phe Val Asn Ala Asp Ser Gly Glu Gly Tyr Ile
            500                 505                 510         
Asn Val Asp Gly Asn Leu Gly Asp Arg Arg Asn Leu Thr Leu Trp Arg
        515                 520                 525             
Asn Gly Asp Asn Val Ile Lys Ala Ala Ala Ser Asn Cys Asn Asn Thr
    530                 535                 540                 
Ile Val Val Ile His Ser Val Gly Pro Val Leu Val Asn Glu Trp Tyr
545                 550                 555                 560 
Asp Asn Pro Asn Val Thr Ala Ile Leu Trp Gly Gly Leu Pro Gly Gln
                565                 570                 575     
Glu Ser Gly Asn Ser Leu Ala Asp Val Leu Tyr Gly Arg Val Asn Pro
            580                 585                 590         
Gly Ala Lys Ser Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln
        595                 600                 605             
Asp Tyr Leu Val Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln Glu
    610                 615                 620                 
Asp Phe Val Glu Gly Val Phe Ile Asp Tyr Arg Gly Phe Asp Lys Arg
625                 630                 635                 640 
Asn Glu Thr Pro Ile Tyr Glu Phe Gly Tyr Gly Leu Ser Tyr Thr Thr
                645                 650                 655     
Phe Asn Tyr Ser Asn Leu Glu Val Gln Val Leu Ser Ala Pro Ala Tyr
            660                 665                 670         
Glu Pro Ala Ser Gly Glu Thr Glu Ala Ala Pro Thr Phe Gly Glu Val
        675                 680                 685             
Gly Asn Ala Ser Asp Tyr Leu Tyr Pro Ser Gly Leu Gln Arg Ile Thr
    690                 695                 700                 
Lys Phe Ile Tyr Pro Trp Leu Asn Gly Thr Asp Leu Glu Ala Ser Ser
705                 710                 715                 720 
Gly Asp Ala Ser Tyr Gly Gln Asp Ser Ser Asp Tyr Leu Pro Glu Gly
                725                 730                 735     
Ala Thr Asp Gly Ser Ala Gln Pro Ile Leu Pro Ala Gly Gly Gly Pro
            740                 745                 750         
Gly Gly Asn Pro Arg Leu Tyr Asp Glu Leu Ile Arg Val Ser Val Thr
        755                 760                 765             
Ile Lys Asn Thr Gly Lys Val Ala Gly Asp Glu Val Pro Gln Leu Tyr
    770                 775                 780                 
Val Ser Leu Gly Gly Pro Asn Glu Pro Lys Ile Val Leu Arg Gln Phe
785                 790                 795                 800 
Glu Arg Ile Thr Leu Gln Pro Ser Glu Glu Thr Lys Trp Ser Thr Thr
                805                 810                 815     
Leu Thr Arg Arg Asp Leu Ala Asn Trp Asn Val Glu Lys Gln Asp Trp
            820                 825                 830         
Glu Ile Thr Ser Tyr Pro Lys Met Val Phe Val Gly Ser Ser Ser Arg
        835                 840                 845             
Lys Leu Pro Leu Arg Ala Ser Leu Pro Thr Val His
    850                 855                 860 


<210> 3
<211> 2580
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG
      acid/base mutant #8, yEdQ885 ->
      Y363C/Y375F/A446A/V498V/G510G/Y560Y/Y610F

<400> 3
atgagattta ctttgattga agctgttgct ttgactgctg tttctttggc ttctgctgat 60
gaattggctt attctccacc atattatcca tctccatggg ctaatggtca aggtgattgg 120
gctcaagctt atcaaagagc tgttgatatt gtttctcaaa tgactttgga tgaaaaagtt 180
aatttgacta ctggtactgg ttgggaattg gaattgtgtg ttggtcaaac tggtggtgtt 240
ccaagattgg gtgttccagg tatgtgtttg caagattctc cattgggtgt tagagattct 300
gattataatt ctgcttttcc agctggtatg aatgttgctg ctacttggga taaaaatttg 360
gcttatttga gaggtaaagc tatgggtcaa gaattttctg ataaaggtgc tgatattcaa 420
ttgggtccag ctgctggtcc attgggtaga tctccagatg gtggtagaaa ttgggaaggt 480
ttttctccag atccagcttt gtctggtgtt ttgtttgctg aaactattaa aggtattcaa 540
gatgctggtg ttgttgctac tgctaaacat tatattgctt atgaacaaga acattttaga 600
caagctccag aagctcaagg ttttggtttt aatatttctg aatctggttc tgctaatttg 660
gatgataaaa ctatgcatga attgtatttg tggccatttg ctgatgctat tagagctggt 720
gctggtgctg ttatgtgttc ttataatcaa attaataatt cttatggttg tcaaaattct 780
tatactttga ataaattgtt gaaagctgaa ttgggttttc aaggttttgt tatgtctgat 840
tgggctgctc atcatgctgg tgtttctggt gctttggctg gtttggatat gtctatgcca 900
ggtgatgttg attatgattc tggtacttct tattggggta ctaatttgac tatttctgtt 960
ttgaatggta ctgttccaca atggagagtt gatgatatgg ctgttagaat tatggctgct 1020
tattataaag ttggtagaga tagattgtgg actccaccaa atttttcttc ttggactaga 1080
gatgaatgtg gttataaata ttattatgtt tctgaaggtc catttgaaaa agttaatcaa 1140
tatgttaatg ttcaaagaaa tcattctgaa ttgattagaa gaattggtgc tgattctact 1200
gttttgttga aaaatgatgg tgctttgcca ttgactggta aagaaagatt ggttgctttg 1260
attggtgaag atgctggttc taatccatat ggtgctaatg gttgttctga tagaggttgt 1320
gataatggta ctttggcgat gggttggggt tctggtactg ctaattttcc atatttggtt 1380
actccagaac aagctatttc taatgaagtt ttgaaacata aaaatggtgt ttttactgct 1440
actgataatt gggctattga tcaaattgaa gctttggcta aaactgcttc tgtatctttg 1500
gtttttgtta atgctgattc tggtgaaggc tatattaatg ttgatggtaa tttgggtgat 1560
agaagaaatt tgactttgtg gagaaatggt gataatgtta ttaaagctgc tgcttctaat 1620
tgtaataata ctattgttgt tattcattct gttggtccag ttttggttaa tgaatggtac 1680
gataatccaa atgttactgc tattttgtgg ggtggtttgc caggtcaaga atctggtaat 1740
tctttggctg atgttttgta tggtagagtt aatccaggtg ctaaatctcc atttacttgg 1800
ggtaaaacta gagaagctta tcaagatttt ttggttactg aaccaaataa tggtaatggt 1860
gctccacaag aagattttgt tgaaggtgtt tttattgatt atagaggttt tgataaaaga 1920
aatgaaactc caatttatga atttggttat ggtttgtctt atactacttt taattattct 1980
aatttggaag ttcaagtttt gtctgctcca gcttatgaac cagcttctgg tgaaactgaa 2040
gctgctccaa cttttggtga agttggtaat gcttctgatt atttgtatcc atctggtttg 2100
caaagaatta ctaaatttat ttatccatgg ttgaatggta ctgatttgga agcttcttct 2160
ggtgatgctt cttatggtca agattcttct gattatttgc cagaaggtgc tactgatggt 2220
tctgctcaac caattttgcc agctggtggt ggtccaggtg gtaatccaag attgtatgat 2280
gaattgatta gagtttctgt tactattaaa aatactggta aagttgctgg tgatgaagtt 2340
ccacaattgt atgtttcttt gggtggtcca aatgaaccaa aaattgtttt gagacaattt 2400
gaaagaatta ctttgcaacc atctgaagaa actaaatggt ctactacttt gactagaaga 2460
gatttggcta attggaatgt tgaaaaacaa gattgggaaa ttacttctta tccaaaaatg 2520
gtttttgttg gttcttcttc tagaaaattg ccattgagag cttctttgcc aactgttcat 2580


<210> 4
<211> 860
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG
      acid/base mutant #8, yEdQ885 ->
      Y363C/Y375F/A446A/V498V/G510G/Y560Y/Y610F

<400> 4
Met Arg Phe Thr Leu Ile Glu Ala Val Ala Leu Thr Ala Val Ser Leu
 1               5                  10                  15      
Ala Ser Ala Asp Glu Leu Ala Tyr Ser Pro Pro Tyr Tyr Pro Ser Pro
            20                  25                  30          
Trp Ala Asn Gly Gln Gly Asp Trp Ala Gln Ala Tyr Gln Arg Ala Val
        35                  40                  45              
Asp Ile Val Ser Gln Met Thr Leu Asp Glu Lys Val Asn Leu Thr Thr
    50                  55                  60                  
Gly Thr Gly Trp Glu Leu Glu Leu Cys Val Gly Gln Thr Gly Gly Val
65                  70                  75                  80  
Pro Arg Leu Gly Val Pro Gly Met Cys Leu Gln Asp Ser Pro Leu Gly
                85                  90                  95      
Val Arg Asp Ser Asp Tyr Asn Ser Ala Phe Pro Ala Gly Met Asn Val
            100                 105                 110         
Ala Ala Thr Trp Asp Lys Asn Leu Ala Tyr Leu Arg Gly Lys Ala Met
        115                 120                 125             
Gly Gln Glu Phe Ser Asp Lys Gly Ala Asp Ile Gln Leu Gly Pro Ala
    130                 135                 140                 
Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn Trp Glu Gly
145                 150                 155                 160 
Phe Ser Pro Asp Pro Ala Leu Ser Gly Val Leu Phe Ala Glu Thr Ile
                165                 170                 175     
Lys Gly Ile Gln Asp Ala Gly Val Val Ala Thr Ala Lys His Tyr Ile
            180                 185                 190         
Ala Tyr Glu Gln Glu His Phe Arg Gln Ala Pro Glu Ala Gln Gly Phe
        195                 200                 205             
Gly Phe Asn Ile Ser Glu Ser Gly Ser Ala Asn Leu Asp Asp Lys Thr
    210                 215                 220                 
Met His Glu Leu Tyr Leu Trp Pro Phe Ala Asp Ala Ile Arg Ala Gly
225                 230                 235                 240 
Ala Gly Ala Val Met Cys Ser Tyr Asn Gln Ile Asn Asn Ser Tyr Gly
                245                 250                 255     
Cys Gln Asn Ser Tyr Thr Leu Asn Lys Leu Leu Lys Ala Glu Leu Gly
            260                 265                 270         
Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Ala Gly Val
        275                 280                 285             
Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp Val Asp
    290                 295                 300                 
Tyr Asp Ser Gly Thr Ser Tyr Trp Gly Thr Asn Leu Thr Ile Ser Val
305                 310                 315                 320 
Leu Asn Gly Thr Val Pro Gln Trp Arg Val Asp Asp Met Ala Val Arg
                325                 330                 335     
Ile Met Ala Ala Tyr Tyr Lys Val Gly Arg Asp Arg Leu Trp Thr Pro
            340                 345                 350         
Pro Asn Phe Ser Ser Trp Thr Arg Asp Glu Cys Gly Tyr Lys Tyr Tyr
        355                 360                 365             
Tyr Val Ser Glu Gly Pro Phe Glu Lys Val Asn Gln Tyr Val Asn Val
    370                 375                 380                 
Gln Arg Asn His Ser Glu Leu Ile Arg Arg Ile Gly Ala Asp Ser Thr
385                 390                 395                 400 
Val Leu Leu Lys Asn Asp Gly Ala Leu Pro Leu Thr Gly Lys Glu Arg
                405                 410                 415     
Leu Val Ala Leu Ile Gly Glu Asp Ala Gly Ser Asn Pro Tyr Gly Ala
            420                 425                 430         
Asn Gly Cys Ser Asp Arg Gly Cys Asp Asn Gly Thr Leu Ala Met Gly
        435                 440                 445             
Trp Gly Ser Gly Thr Ala Asn Phe Pro Tyr Leu Val Thr Pro Glu Gln
    450                 455                 460                 
Ala Ile Ser Asn Glu Val Leu Lys His Lys Asn Gly Val Phe Thr Ala
465                 470                 475                 480 
Thr Asp Asn Trp Ala Ile Asp Gln Ile Glu Ala Leu Ala Lys Thr Ala
                485                 490                 495     
Ser Val Ser Leu Val Phe Val Asn Ala Asp Ser Gly Glu Gly Tyr Ile
            500                 505                 510         
Asn Val Asp Gly Asn Leu Gly Asp Arg Arg Asn Leu Thr Leu Trp Arg
        515                 520                 525             
Asn Gly Asp Asn Val Ile Lys Ala Ala Ala Ser Asn Cys Asn Asn Thr
    530                 535                 540                 
Ile Val Val Ile His Ser Val Gly Pro Val Leu Val Asn Glu Trp Tyr
545                 550                 555                 560 
Asp Asn Pro Asn Val Thr Ala Ile Leu Trp Gly Gly Leu Pro Gly Gln
                565                 570                 575     
Glu Ser Gly Asn Ser Leu Ala Asp Val Leu Tyr Gly Arg Val Asn Pro
            580                 585                 590         
Gly Ala Lys Ser Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln
        595                 600                 605             
Asp Phe Leu Val Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln Glu
    610                 615                 620                 
Asp Phe Val Glu Gly Val Phe Ile Asp Tyr Arg Gly Phe Asp Lys Arg
625                 630                 635                 640 
Asn Glu Thr Pro Ile Tyr Glu Phe Gly Tyr Gly Leu Ser Tyr Thr Thr
                645                 650                 655     
Phe Asn Tyr Ser Asn Leu Glu Val Gln Val Leu Ser Ala Pro Ala Tyr
            660                 665                 670         
Glu Pro Ala Ser Gly Glu Thr Glu Ala Ala Pro Thr Phe Gly Glu Val
        675                 680                 685             
Gly Asn Ala Ser Asp Tyr Leu Tyr Pro Ser Gly Leu Gln Arg Ile Thr
    690                 695                 700                 
Lys Phe Ile Tyr Pro Trp Leu Asn Gly Thr Asp Leu Glu Ala Ser Ser
705                 710                 715                 720 
Gly Asp Ala Ser Tyr Gly Gln Asp Ser Ser Asp Tyr Leu Pro Glu Gly
                725                 730                 735     
Ala Thr Asp Gly Ser Ala Gln Pro Ile Leu Pro Ala Gly Gly Gly Pro
            740                 745                 750         
Gly Gly Asn Pro Arg Leu Tyr Asp Glu Leu Ile Arg Val Ser Val Thr
        755                 760                 765             
Ile Lys Asn Thr Gly Lys Val Ala Gly Asp Glu Val Pro Gln Leu Tyr
    770                 775                 780                 
Val Ser Leu Gly Gly Pro Asn Glu Pro Lys Ile Val Leu Arg Gln Phe
785                 790                 795                 800 
Glu Arg Ile Thr Leu Gln Pro Ser Glu Glu Thr Lys Trp Ser Thr Thr
                805                 810                 815     
Leu Thr Arg Arg Asp Leu Ala Asn Trp Asn Val Glu Lys Gln Asp Trp
            820                 825                 830         
Glu Ile Thr Ser Tyr Pro Lys Met Val Phe Val Gly Ser Ser Ser Arg
        835                 840                 845             
Lys Leu Pro Leu Arg Ala Ser Leu Pro Thr Val His
    850                 855                 860 


<210> 5
<211> 2580
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #1, yEdQ890 -> L70S/I139F

<400> 5
atgagattta ctttgattga agctgttgct ttgactgctg tttctttggc ttctgctgat 60
gaattggctt attctccacc atattatcca tctccatggg ctaatggtca aggtgattgg 120
gctcaagctt atcaaagagc tgttgatatt gtttctcaaa tgactttgga tgaaaaagtt 180
aatttgacta ctggtactgg ttgggaatcg gaattgtgtg ttggtcaaac tggtggtgtt 240
ccaagattgg gtgttccagg tatgtgtttg caagattctc cattgggtgt tagagattct 300
gattataatt ctgcttttcc agctggtatg aatgttgctg ctacttggga taaaaatttg 360
gcttatttga gaggtaaagc tatgggtcaa gaattttctg ataaaggtgc tgattttcaa 420
ttgggtccag ctgctggtcc attgggtaga tctccagatg gtggtagaaa ttgggaaggt 480
ttttctccag atccagcttt gtctggtgtt ttgtttgctg aaactattaa aggtattcaa 540
gatgctggtg ttgttgctac tgctaaacat tatattgctt atgaacaaga acattttaga 600
caagctccag aagctcaagg ttttggtttt aatatttctg aatctggttc tgctaatttg 660
gatgataaaa ctatgcatga attgtatttg tggccatttg ctgatgctat tagagctggt 720
gctggtgctg ttatgtgttc ttataatcaa attaataatt cttatggttg tcaaaattct 780
tatactttga ataaattgtt gaaagctgaa ttgggttttc aaggttttgt tatgtctgat 840
tgggctgctc atcatgctgg tgtttctggt gctttggctg gtttggatat gtctatgcca 900
ggtgatgttg attatgattc tggtacttct tattggggta ctaatttgac tatttctgtt 960
ttgaatggta ctgttccaca atggagagtt gatgatatgg ctgttagaat tatggctgct 1020
tattataaag ttggtagaga tagattgtgg actccaccaa atttttcttc ttggactaga 1080
gatgaatatg gttataaata ttattatgtt tctgaaggtc catatgaaaa agttaatcaa 1140
tatgttaatg ttcaaagaaa tcattctgaa ttgattagaa gaattggtgc tgattctact 1200
gttttgttga aaaatgatgg tgctttgcca ttgactggta aagaaagatt ggttgctttg 1260
attggtgaag atgctggttc taatccatat ggtgctaatg gttgttctga tagaggttgt 1320
gataatggta ctttggctat gggttggggt tctggtactg ctaattttcc atatttggtt 1380
actccagaac aagctatttc taatgaagtt ttgaaacata aaaatggtgt ttttactgct 1440
actgataatt gggctattga tcaaattgaa gctttggcta aaactgcttc tgtttctttg 1500
gtttttgtta atgctgattc tggtgaaggt tatattaatg ttgatggtaa tttgggtgat 1560
agaagaaatt tgactttgtg gagaaatggt gataatgtta ttaaagctgc tgcttctaat 1620
tgtaataata ctattgttgt tattcattct gttggtccag ttttggttaa tgaatggtat 1680
gataatccaa atgttactgc tattttgtgg ggtggtttgc caggtcaaga atctggtaat 1740
tctttggctg atgttttgta tggtagagtt aatccaggtg ctaaatctcc atttacttgg 1800
ggtaaaacta gagaagctta tcaagattat ttggttactg aaccaaataa tggtaatggt 1860
gctccacaag aagattttgt tgaaggtgtt tttattgatt atagaggttt tgataaaaga 1920
aatgaaactc caatttatga atttggttat ggtttgtctt atactacttt taattattct 1980
aatttggaag ttcaagtttt gtctgctcca gcttatgaac cagcttctgg tgaaactgaa 2040
gctgctccaa cttttggtga agttggtaat gcttctgatt atttgtatcc atctggtttg 2100
caaagaatta ctaaatttat ttatccatgg ttgaatggta ctgatttgga agcttcttct 2160
ggtgatgctt cttatggtca agattcttct gattatttgc cagaaggtgc tactgatggt 2220
tctgctcaac caattttgcc agctggtggt ggtccaggtg gtaatccaag attgtatgat 2280
gaattgatta gagtttctgt tactattaaa aatactggta aagttgctgg tgatgaagtt 2340
ccacaattgt atgtttcttt gggtggtcca aatgaaccaa aaattgtttt gagacaattt 2400
gaaagaatta ctttgcaacc atctgaagaa actaaatggt ctactacttt gactagaaga 2460
gatttggcta attggaatgt tgaaaaacaa gattgggaaa ttacttctta tccaaaaatg 2520
gtttttgttg gttcttcttc tagaaaattg ccattgagag cttctttgcc aactgttcat 2580


<210> 6
<211> 860
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #1, yEdQ890 -> L70S/I139F

<400> 6
Met Arg Phe Thr Leu Ile Glu Ala Val Ala Leu Thr Ala Val Ser Leu
 1               5                  10                  15      
Ala Ser Ala Asp Glu Leu Ala Tyr Ser Pro Pro Tyr Tyr Pro Ser Pro
            20                  25                  30          
Trp Ala Asn Gly Gln Gly Asp Trp Ala Gln Ala Tyr Gln Arg Ala Val
        35                  40                  45              
Asp Ile Val Ser Gln Met Thr Leu Asp Glu Lys Val Asn Leu Thr Thr
    50                  55                  60                  
Gly Thr Gly Trp Glu Ser Glu Leu Cys Val Gly Gln Thr Gly Gly Val
65                  70                  75                  80  
Pro Arg Leu Gly Val Pro Gly Met Cys Leu Gln Asp Ser Pro Leu Gly
                85                  90                  95      
Val Arg Asp Ser Asp Tyr Asn Ser Ala Phe Pro Ala Gly Met Asn Val
            100                 105                 110         
Ala Ala Thr Trp Asp Lys Asn Leu Ala Tyr Leu Arg Gly Lys Ala Met
        115                 120                 125             
Gly Gln Glu Phe Ser Asp Lys Gly Ala Asp Phe Gln Leu Gly Pro Ala
    130                 135                 140                 
Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn Trp Glu Gly
145                 150                 155                 160 
Phe Ser Pro Asp Pro Ala Leu Ser Gly Val Leu Phe Ala Glu Thr Ile
                165                 170                 175     
Lys Gly Ile Gln Asp Ala Gly Val Val Ala Thr Ala Lys His Tyr Ile
            180                 185                 190         
Ala Tyr Glu Gln Glu His Phe Arg Gln Ala Pro Glu Ala Gln Gly Phe
        195                 200                 205             
Gly Phe Asn Ile Ser Glu Ser Gly Ser Ala Asn Leu Asp Asp Lys Thr
    210                 215                 220                 
Met His Glu Leu Tyr Leu Trp Pro Phe Ala Asp Ala Ile Arg Ala Gly
225                 230                 235                 240 
Ala Gly Ala Val Met Cys Ser Tyr Asn Gln Ile Asn Asn Ser Tyr Gly
                245                 250                 255     
Cys Gln Asn Ser Tyr Thr Leu Asn Lys Leu Leu Lys Ala Glu Leu Gly
            260                 265                 270         
Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Ala Gly Val
        275                 280                 285             
Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp Val Asp
    290                 295                 300                 
Tyr Asp Ser Gly Thr Ser Tyr Trp Gly Thr Asn Leu Thr Ile Ser Val
305                 310                 315                 320 
Leu Asn Gly Thr Val Pro Gln Trp Arg Val Asp Asp Met Ala Val Arg
                325                 330                 335     
Ile Met Ala Ala Tyr Tyr Lys Val Gly Arg Asp Arg Leu Trp Thr Pro
            340                 345                 350         
Pro Asn Phe Ser Ser Trp Thr Arg Asp Glu Tyr Gly Tyr Lys Tyr Tyr
        355                 360                 365             
Tyr Val Ser Glu Gly Pro Tyr Glu Lys Val Asn Gln Tyr Val Asn Val
    370                 375                 380                 
Gln Arg Asn His Ser Glu Leu Ile Arg Arg Ile Gly Ala Asp Ser Thr
385                 390                 395                 400 
Val Leu Leu Lys Asn Asp Gly Ala Leu Pro Leu Thr Gly Lys Glu Arg
                405                 410                 415     
Leu Val Ala Leu Ile Gly Glu Asp Ala Gly Ser Asn Pro Tyr Gly Ala
            420                 425                 430         
Asn Gly Cys Ser Asp Arg Gly Cys Asp Asn Gly Thr Leu Ala Met Gly
        435                 440                 445             
Trp Gly Ser Gly Thr Ala Asn Phe Pro Tyr Leu Val Thr Pro Glu Gln
    450                 455                 460                 
Ala Ile Ser Asn Glu Val Leu Lys His Lys Asn Gly Val Phe Thr Ala
465                 470                 475                 480 
Thr Asp Asn Trp Ala Ile Asp Gln Ile Glu Ala Leu Ala Lys Thr Ala
                485                 490                 495     
Ser Val Ser Leu Val Phe Val Asn Ala Asp Ser Gly Glu Gly Tyr Ile
            500                 505                 510         
Asn Val Asp Gly Asn Leu Gly Asp Arg Arg Asn Leu Thr Leu Trp Arg
        515                 520                 525             
Asn Gly Asp Asn Val Ile Lys Ala Ala Ala Ser Asn Cys Asn Asn Thr
    530                 535                 540                 
Ile Val Val Ile His Ser Val Gly Pro Val Leu Val Asn Glu Trp Tyr
545                 550                 555                 560 
Asp Asn Pro Asn Val Thr Ala Ile Leu Trp Gly Gly Leu Pro Gly Gln
                565                 570                 575     
Glu Ser Gly Asn Ser Leu Ala Asp Val Leu Tyr Gly Arg Val Asn Pro
            580                 585                 590         
Gly Ala Lys Ser Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln
        595                 600                 605             
Asp Tyr Leu Val Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln Glu
    610                 615                 620                 
Asp Phe Val Glu Gly Val Phe Ile Asp Tyr Arg Gly Phe Asp Lys Arg
625                 630                 635                 640 
Asn Glu Thr Pro Ile Tyr Glu Phe Gly Tyr Gly Leu Ser Tyr Thr Thr
                645                 650                 655     
Phe Asn Tyr Ser Asn Leu Glu Val Gln Val Leu Ser Ala Pro Ala Tyr
            660                 665                 670         
Glu Pro Ala Ser Gly Glu Thr Glu Ala Ala Pro Thr Phe Gly Glu Val
        675                 680                 685             
Gly Asn Ala Ser Asp Tyr Leu Tyr Pro Ser Gly Leu Gln Arg Ile Thr
    690                 695                 700                 
Lys Phe Ile Tyr Pro Trp Leu Asn Gly Thr Asp Leu Glu Ala Ser Ser
705                 710                 715                 720 
Gly Asp Ala Ser Tyr Gly Gln Asp Ser Ser Asp Tyr Leu Pro Glu Gly
                725                 730                 735     
Ala Thr Asp Gly Ser Ala Gln Pro Ile Leu Pro Ala Gly Gly Gly Pro
            740                 745                 750         
Gly Gly Asn Pro Arg Leu Tyr Asp Glu Leu Ile Arg Val Ser Val Thr
        755                 760                 765             
Ile Lys Asn Thr Gly Lys Val Ala Gly Asp Glu Val Pro Gln Leu Tyr
    770                 775                 780                 
Val Ser Leu Gly Gly Pro Asn Glu Pro Lys Ile Val Leu Arg Gln Phe
785                 790                 795                 800 
Glu Arg Ile Thr Leu Gln Pro Ser Glu Glu Thr Lys Trp Ser Thr Thr
                805                 810                 815     
Leu Thr Arg Arg Asp Leu Ala Asn Trp Asn Val Glu Lys Gln Asp Trp
            820                 825                 830         
Glu Ile Thr Ser Tyr Pro Lys Met Val Phe Val Gly Ser Ser Ser Arg
        835                 840                 845             
Lys Leu Pro Leu Arg Ala Ser Leu Pro Thr Val His
    850                 855                 860 


<210> 7
<211> 2580
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #6, yEdQ891 -> P152P/L167M/A286D/T835T

<400> 7
atgagattta ctttgattga agctgttgct ttgactgctg tttctttggc ttctgctgat 60
gaattggctt attctccacc atattatcca tctccatggg ctaatggtca aggtgattgg 120
gctcaagctt atcaaagagc tgttgatatt gtttctcaaa tgactttgga tgaaaaagtt 180
aatttgacta ctggtactgg ttgggaattg gaattgtgtg ttggtcaaac tggtggtgtt 240
ccaagattgg gtgttccagg tatgtgtttg caagattctc cattgggtgt tagagattct 300
gattataatt ctgcttttcc agctggtatg aatgttgctg ctacttggga taaaaatttg 360
gcttatttga gaggtaaagc tatgggtcaa gaattttctg ataaaggtgc tgatattcaa 420
ttgggtccag ctgctggtcc attgggtaga tctcctgatg gtggtagaaa ttgggaaggt 480
ttttctccag atccagctat gtctggtgtt ttgtttgctg aaactattaa aggtattcaa 540
gatgctggtg ttgttgctac tgctaaacat tatattgctt atgaacaaga acattttaga 600
caagctccag aagctcaagg ttttggtttt aatatttctg aatctggttc tgctaatttg 660
gatgataaaa ctatgcatga attgtatttg tggccatttg ctgatgctat tagagctggt 720
gctggtgctg ttatgtgttc ttataatcaa attaataatt cttatggttg tcaaaattct 780
tatactttga ataaattgtt gaaagctgaa ttgggttttc aaggttttgt tatgtctgat 840
tgggctgctc atcatgatgg tgtttctggt gctttggctg gtttggatat gtctatgcca 900
ggtgatgttg attatgattc tggtacttct tattggggta ctaatttgac tatttctgtt 960
ttgaatggta ctgttccaca atggagagtt gatgatatgg ctgttagaat tatggctgct 1020
tattataaag ttggtagaga tagattgtgg actccaccaa atttttcttc ttggactaga 1080
gatgaatatg gttataaata ttattatgtt tctgaaggtc catatgaaaa agttaatcaa 1140
tatgttaatg ttcaaagaaa tcattctgaa ttgattagaa gaattggtgc tgattctact 1200
gttttgttga aaaatgatgg tgctttgcca ttgactggta aagaaagatt ggttgctttg 1260
attggtgaag atgctggttc taatccatat ggtgctaatg gttgttctga tagaggttgt 1320
gataatggta ctttggctat gggttggggt tctggtactg ctaattttcc atatttggtt 1380
actccagaac aagctatttc taatgaagtt ttgaaacata aaaatggtgt ttttactgct 1440
actgataatt gggctattga tcaaattgaa gctttggcta aaactgcttc tgtttctttg 1500
gtttttgtta atgctgattc tggtgaaggt tatattaatg ttgatggtaa tttgggtgat 1560
agaagaaatt tgactttgtg gagaaatggt gataatgtta ttaaagctgc tgcttctaat 1620
tgtaataata ctattgttgt tattcattct gttggtccag ttttggttaa tgaatggtat 1680
gataatccaa atgttactgc tattttgtgg ggtggtttgc caggtcaaga atctggtaat 1740
tctttggctg atgttttgta tggtagagtt aatccaggtg ctaaatctcc atttacttgg 1800
ggtaaaacta gagaagctta tcaagattat ttggttactg aaccaaataa tggtaatggt 1860
gctccacaag aagattttgt tgaaggtgtt tttattgatt atagaggttt tgataaaaga 1920
aatgaaactc caatttatga atttggttat ggtttgtctt atactacttt taattattct 1980
aatttggaag ttcaagtttt gtctgctcca gcttatgaac cagcttctgg tgaaactgaa 2040
gctgctccaa cttttggtga agttggtaat gcttctgatt atttgtatcc atctggtttg 2100
caaagaatta ctaaatttat ttatccatgg ttgaatggta ctgatttgga agcttcttct 2160
ggtgatgctt cttatggtca agattcttct gattatttgc cagaaggtgc tactgatggt 2220
tctgctcaac caattttgcc agctggtggt ggtccaggtg gtaatccaag attgtatgat 2280
gaattgatta gagtttctgt tactattaaa aatactggta aagttgctgg tgatgaagtt 2340
ccacaattgt atgtttcttt gggtggtcca aatgaaccaa aaattgtttt gagacaattt 2400
gaaagaatta ctttgcaacc atctgaagaa actaaatggt ctactacttt gactagaaga 2460
gatttggcta attggaatgt tgaaaaacaa gattgggaaa ttacatctta tccaaaaatg 2520
gtttttgttg gttcttcttc tagaaaattg ccattgagag cttctttgcc aactgttcat 2580


<210> 8
<211> 860
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #6, yEdQ891 -> P152P/L167M/A286D/T835T

<400> 8
Met Arg Phe Thr Leu Ile Glu Ala Val Ala Leu Thr Ala Val Ser Leu
 1               5                  10                  15      
Ala Ser Ala Asp Glu Leu Ala Tyr Ser Pro Pro Tyr Tyr Pro Ser Pro
            20                  25                  30          
Trp Ala Asn Gly Gln Gly Asp Trp Ala Gln Ala Tyr Gln Arg Ala Val
        35                  40                  45              
Asp Ile Val Ser Gln Met Thr Leu Asp Glu Lys Val Asn Leu Thr Thr
    50                  55                  60                  
Gly Thr Gly Trp Glu Leu Glu Leu Cys Val Gly Gln Thr Gly Gly Val
65                  70                  75                  80  
Pro Arg Leu Gly Val Pro Gly Met Cys Leu Gln Asp Ser Pro Leu Gly
                85                  90                  95      
Val Arg Asp Ser Asp Tyr Asn Ser Ala Phe Pro Ala Gly Met Asn Val
            100                 105                 110         
Ala Ala Thr Trp Asp Lys Asn Leu Ala Tyr Leu Arg Gly Lys Ala Met
        115                 120                 125             
Gly Gln Glu Phe Ser Asp Lys Gly Ala Asp Ile Gln Leu Gly Pro Ala
    130                 135                 140                 
Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn Trp Glu Gly
145                 150                 155                 160 
Phe Ser Pro Asp Pro Ala Met Ser Gly Val Leu Phe Ala Glu Thr Ile
                165                 170                 175     
Lys Gly Ile Gln Asp Ala Gly Val Val Ala Thr Ala Lys His Tyr Ile
            180                 185                 190         
Ala Tyr Glu Gln Glu His Phe Arg Gln Ala Pro Glu Ala Gln Gly Phe
        195                 200                 205             
Gly Phe Asn Ile Ser Glu Ser Gly Ser Ala Asn Leu Asp Asp Lys Thr
    210                 215                 220                 
Met His Glu Leu Tyr Leu Trp Pro Phe Ala Asp Ala Ile Arg Ala Gly
225                 230                 235                 240 
Ala Gly Ala Val Met Cys Ser Tyr Asn Gln Ile Asn Asn Ser Tyr Gly
                245                 250                 255     
Cys Gln Asn Ser Tyr Thr Leu Asn Lys Leu Leu Lys Ala Glu Leu Gly
            260                 265                 270         
Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Asp Gly Val
        275                 280                 285             
Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp Val Asp
    290                 295                 300                 
Tyr Asp Ser Gly Thr Ser Tyr Trp Gly Thr Asn Leu Thr Ile Ser Val
305                 310                 315                 320 
Leu Asn Gly Thr Val Pro Gln Trp Arg Val Asp Asp Met Ala Val Arg
                325                 330                 335     
Ile Met Ala Ala Tyr Tyr Lys Val Gly Arg Asp Arg Leu Trp Thr Pro
            340                 345                 350         
Pro Asn Phe Ser Ser Trp Thr Arg Asp Glu Tyr Gly Tyr Lys Tyr Tyr
        355                 360                 365             
Tyr Val Ser Glu Gly Pro Tyr Glu Lys Val Asn Gln Tyr Val Asn Val
    370                 375                 380                 
Gln Arg Asn His Ser Glu Leu Ile Arg Arg Ile Gly Ala Asp Ser Thr
385                 390                 395                 400 
Val Leu Leu Lys Asn Asp Gly Ala Leu Pro Leu Thr Gly Lys Glu Arg
                405                 410                 415     
Leu Val Ala Leu Ile Gly Glu Asp Ala Gly Ser Asn Pro Tyr Gly Ala
            420                 425                 430         
Asn Gly Cys Ser Asp Arg Gly Cys Asp Asn Gly Thr Leu Ala Met Gly
        435                 440                 445             
Trp Gly Ser Gly Thr Ala Asn Phe Pro Tyr Leu Val Thr Pro Glu Gln
    450                 455                 460                 
Ala Ile Ser Asn Glu Val Leu Lys His Lys Asn Gly Val Phe Thr Ala
465                 470                 475                 480 
Thr Asp Asn Trp Ala Ile Asp Gln Ile Glu Ala Leu Ala Lys Thr Ala
                485                 490                 495     
Ser Val Ser Leu Val Phe Val Asn Ala Asp Ser Gly Glu Gly Tyr Ile
            500                 505                 510         
Asn Val Asp Gly Asn Leu Gly Asp Arg Arg Asn Leu Thr Leu Trp Arg
        515                 520                 525             
Asn Gly Asp Asn Val Ile Lys Ala Ala Ala Ser Asn Cys Asn Asn Thr
    530                 535                 540                 
Ile Val Val Ile His Ser Val Gly Pro Val Leu Val Asn Glu Trp Tyr
545                 550                 555                 560 
Asp Asn Pro Asn Val Thr Ala Ile Leu Trp Gly Gly Leu Pro Gly Gln
                565                 570                 575     
Glu Ser Gly Asn Ser Leu Ala Asp Val Leu Tyr Gly Arg Val Asn Pro
            580                 585                 590         
Gly Ala Lys Ser Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln
        595                 600                 605             
Asp Tyr Leu Val Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln Glu
    610                 615                 620                 
Asp Phe Val Glu Gly Val Phe Ile Asp Tyr Arg Gly Phe Asp Lys Arg
625                 630                 635                 640 
Asn Glu Thr Pro Ile Tyr Glu Phe Gly Tyr Gly Leu Ser Tyr Thr Thr
                645                 650                 655     
Phe Asn Tyr Ser Asn Leu Glu Val Gln Val Leu Ser Ala Pro Ala Tyr
            660                 665                 670         
Glu Pro Ala Ser Gly Glu Thr Glu Ala Ala Pro Thr Phe Gly Glu Val
        675                 680                 685             
Gly Asn Ala Ser Asp Tyr Leu Tyr Pro Ser Gly Leu Gln Arg Ile Thr
    690                 695                 700                 
Lys Phe Ile Tyr Pro Trp Leu Asn Gly Thr Asp Leu Glu Ala Ser Ser
705                 710                 715                 720 
Gly Asp Ala Ser Tyr Gly Gln Asp Ser Ser Asp Tyr Leu Pro Glu Gly
                725                 730                 735     
Ala Thr Asp Gly Ser Ala Gln Pro Ile Leu Pro Ala Gly Gly Gly Pro
            740                 745                 750         
Gly Gly Asn Pro Arg Leu Tyr Asp Glu Leu Ile Arg Val Ser Val Thr
        755                 760                 765             
Ile Lys Asn Thr Gly Lys Val Ala Gly Asp Glu Val Pro Gln Leu Tyr
    770                 775                 780                 
Val Ser Leu Gly Gly Pro Asn Glu Pro Lys Ile Val Leu Arg Gln Phe
785                 790                 795                 800 
Glu Arg Ile Thr Leu Gln Pro Ser Glu Glu Thr Lys Trp Ser Thr Thr
                805                 810                 815     
Leu Thr Arg Arg Asp Leu Ala Asn Trp Asn Val Glu Lys Gln Asp Trp
            820                 825                 830         
Glu Ile Thr Ser Tyr Pro Lys Met Val Phe Val Gly Ser Ser Ser Arg
        835                 840                 845             
Lys Leu Pro Leu Arg Ala Ser Leu Pro Thr Val His
    850                 855                 860 


<210> 9
<211> 2580
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #A, yEdQ892 -> G136C/E376K/A606A/N791I

<400> 9
atgagattta ctttgattga agctgttgct ttgactgctg tttctttggc ttctgctgat 60
gaattggctt attctccacc atattatcca tctccatggg ctaatggtca aggtgattgg 120
gctcaagctt atcaaagagc tgttgatatt gtttctcaaa tgactttgga tgaaaaagtt 180
aatttgacta ctggtactgg ttgggaattg gaattgtgtg ttggtcaaac tggtggtgtt 240
ccaagattgg gtgttccagg tatgtgtttg caagattctc cattgggtgt tagagattct 300
gattataatt ctgcttttcc agctggtatg aatgttgctg ctacttggga taaaaatttg 360
gcttatttga gaggtaaagc tatgggtcaa gaattttctg ataaatgtgc tgatattcaa 420
ttgggtccag ctgctggtcc attgggtaga tctccagatg gtggtagaaa ttgggaaggt 480
ttttctccag atccagcttt gtctggtgtt ttgtttgctg aaactattaa aggtattcaa 540
gatgctggtg ttgttgctac tgctaaacat tatattgctt atgaacaaga acattttaga 600
caagctccag aagctcaagg ttttggtttt aatatttctg aatctggttc tgctaatttg 660
gatgataaaa ctatgcatga attgtatttg tggccatttg ctgatgctat tagagctggt 720
gctggtgctg ttatgtgttc ttataatcaa attaataatt cttatggttg tcaaaattct 780
tatactttga ataaattgtt gaaagctgaa ttgggttttc aaggttttgt tatgtctgat 840
tgggctgctc atcatgctgg tgtttctggt gctttggctg gtttggatat gtctatgcca 900
ggtgatgttg attatgattc tggtacttct tattggggta ctaatttgac tatttctgtt 960
ttgaatggta ctgttccaca atggagagtt gatgatatgg ctgttagaat tatggctgct 1020
tattataaag ttggtagaga tagattgtgg actccaccaa atttttcttc ttggactaga 1080
gatgaatatg gttataaata ttattatgtt tctgaaggtc catataaaaa agttaatcaa 1140
tatgttaatg ttcaaagaaa tcattctgaa ttgattagaa gaattggtgc tgattctact 1200
gttttgttga aaaatgatgg tgctttgcca ttgactggta aagaaagatt ggttgctttg 1260
attggtgaag atgctggttc taatccatat ggtgctaatg gttgttctga tagaggttgt 1320
gataatggta ctttggctat gggttggggt tctggtactg ctaattttcc atatttggtt 1380
actccagaac aagctatttc taatgaagtt ttgaaacata aaaatggtgt ttttactgct 1440
actgataatt gggctattga tcaaattgaa gctttggcta aaactgcttc tgtttctttg 1500
gtttttgtta atgctgattc tggtgaaggt tatattaatg ttgatggtaa tttgggtgat 1560
agaagaaatt tgactttgtg gagaaatggt gataatgtta ttaaagctgc tgcttctaat 1620
tgtaataata ctattgttgt tattcattct gttggtccag ttttggttaa tgaatggtat 1680
gataatccaa atgttactgc tattttgtgg ggtggtttgc caggtcaaga atctggtaat 1740
tctttggctg atgttttgta tggtagagtt aatccaggtg ctaaatctcc atttacttgg 1800
ggtaaaacta gagaagcata tcaagattat ttggttactg aaccaaataa tggtaatggt 1860
gctccacaag aagattttgt tgaaggtgtt tttattgatt atagaggttt tgataaaaga 1920
aatgaaactc caatttatga atttggttat ggtttgtctt atactacttt taattattct 1980
aatttggaag ttcaagtttt gtctgctcca gcttatgaac cagcttctgg tgaaactgaa 2040
gctgctccaa cttttggtga agttggtaat gcttctgatt atttgtatcc atctggtttg 2100
caaagaatta ctaaatttat ttatccatgg ttgaatggta ctgatttgga agcttcttct 2160
ggtgatgctt cttatggtca agattcttct gattatttgc cagaaggtgc tactgatggt 2220
tctgctcaac caattttgcc agctggtggt ggtccaggtg gtaatccaag attgtatgat 2280
gaattgatta gagtttctgt tactattaaa aatactggta aagttgctgg tgatgaagtt 2340
ccacaattgt atgtttcttt gggtggtcca attgaaccaa aaattgtttt gagacaattt 2400
gaaagaatta ctttgcaacc atctgaagaa actaaatggt ctactacttt gactagaaga 2460
gatttggcta attggaatgt tgaaaaacaa gattgggaaa ttacttctta tccaaaaatg 2520
gtttttgttg gttcttcttc tagaaaattg ccattgagag cttctttgcc aactgttcat 2580


<210> 10
<211> 860
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #A, yEdQ892 -> G136C/E376K/A606A/N791I

<400> 10
Met Arg Phe Thr Leu Ile Glu Ala Val Ala Leu Thr Ala Val Ser Leu
 1               5                  10                  15      
Ala Ser Ala Asp Glu Leu Ala Tyr Ser Pro Pro Tyr Tyr Pro Ser Pro
            20                  25                  30          
Trp Ala Asn Gly Gln Gly Asp Trp Ala Gln Ala Tyr Gln Arg Ala Val
        35                  40                  45              
Asp Ile Val Ser Gln Met Thr Leu Asp Glu Lys Val Asn Leu Thr Thr
    50                  55                  60                  
Gly Thr Gly Trp Glu Leu Glu Leu Cys Val Gly Gln Thr Gly Gly Val
65                  70                  75                  80  
Pro Arg Leu Gly Val Pro Gly Met Cys Leu Gln Asp Ser Pro Leu Gly
                85                  90                  95      
Val Arg Asp Ser Asp Tyr Asn Ser Ala Phe Pro Ala Gly Met Asn Val
            100                 105                 110         
Ala Ala Thr Trp Asp Lys Asn Leu Ala Tyr Leu Arg Gly Lys Ala Met
        115                 120                 125             
Gly Gln Glu Phe Ser Asp Lys Cys Ala Asp Ile Gln Leu Gly Pro Ala
    130                 135                 140                 
Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn Trp Glu Gly
145                 150                 155                 160 
Phe Ser Pro Asp Pro Ala Leu Ser Gly Val Leu Phe Ala Glu Thr Ile
                165                 170                 175     
Lys Gly Ile Gln Asp Ala Gly Val Val Ala Thr Ala Lys His Tyr Ile
            180                 185                 190         
Ala Tyr Glu Gln Glu His Phe Arg Gln Ala Pro Glu Ala Gln Gly Phe
        195                 200                 205             
Gly Phe Asn Ile Ser Glu Ser Gly Ser Ala Asn Leu Asp Asp Lys Thr
    210                 215                 220                 
Met His Glu Leu Tyr Leu Trp Pro Phe Ala Asp Ala Ile Arg Ala Gly
225                 230                 235                 240 
Ala Gly Ala Val Met Cys Ser Tyr Asn Gln Ile Asn Asn Ser Tyr Gly
                245                 250                 255     
Cys Gln Asn Ser Tyr Thr Leu Asn Lys Leu Leu Lys Ala Glu Leu Gly
            260                 265                 270         
Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Ala Gly Val
        275                 280                 285             
Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp Val Asp
    290                 295                 300                 
Tyr Asp Ser Gly Thr Ser Tyr Trp Gly Thr Asn Leu Thr Ile Ser Val
305                 310                 315                 320 
Leu Asn Gly Thr Val Pro Gln Trp Arg Val Asp Asp Met Ala Val Arg
                325                 330                 335     
Ile Met Ala Ala Tyr Tyr Lys Val Gly Arg Asp Arg Leu Trp Thr Pro
            340                 345                 350         
Pro Asn Phe Ser Ser Trp Thr Arg Asp Glu Tyr Gly Tyr Lys Tyr Tyr
        355                 360                 365             
Tyr Val Ser Glu Gly Pro Tyr Lys Lys Val Asn Gln Tyr Val Asn Val
    370                 375                 380                 
Gln Arg Asn His Ser Glu Leu Ile Arg Arg Ile Gly Ala Asp Ser Thr
385                 390                 395                 400 
Val Leu Leu Lys Asn Asp Gly Ala Leu Pro Leu Thr Gly Lys Glu Arg
                405                 410                 415     
Leu Val Ala Leu Ile Gly Glu Asp Ala Gly Ser Asn Pro Tyr Gly Ala
            420                 425                 430         
Asn Gly Cys Ser Asp Arg Gly Cys Asp Asn Gly Thr Leu Ala Met Gly
        435                 440                 445             
Trp Gly Ser Gly Thr Ala Asn Phe Pro Tyr Leu Val Thr Pro Glu Gln
    450                 455                 460                 
Ala Ile Ser Asn Glu Val Leu Lys His Lys Asn Gly Val Phe Thr Ala
465                 470                 475                 480 
Thr Asp Asn Trp Ala Ile Asp Gln Ile Glu Ala Leu Ala Lys Thr Ala
                485                 490                 495     
Ser Val Ser Leu Val Phe Val Asn Ala Asp Ser Gly Glu Gly Tyr Ile
            500                 505                 510         
Asn Val Asp Gly Asn Leu Gly Asp Arg Arg Asn Leu Thr Leu Trp Arg
        515                 520                 525             
Asn Gly Asp Asn Val Ile Lys Ala Ala Ala Ser Asn Cys Asn Asn Thr
    530                 535                 540                 
Ile Val Val Ile His Ser Val Gly Pro Val Leu Val Asn Glu Trp Tyr
545                 550                 555                 560 
Asp Asn Pro Asn Val Thr Ala Ile Leu Trp Gly Gly Leu Pro Gly Gln
                565                 570                 575     
Glu Ser Gly Asn Ser Leu Ala Asp Val Leu Tyr Gly Arg Val Asn Pro
            580                 585                 590         
Gly Ala Lys Ser Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln
        595                 600                 605             
Asp Tyr Leu Val Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln Glu
    610                 615                 620                 
Asp Phe Val Glu Gly Val Phe Ile Asp Tyr Arg Gly Phe Asp Lys Arg
625                 630                 635                 640 
Asn Glu Thr Pro Ile Tyr Glu Phe Gly Tyr Gly Leu Ser Tyr Thr Thr
                645                 650                 655     
Phe Asn Tyr Ser Asn Leu Glu Val Gln Val Leu Ser Ala Pro Ala Tyr
            660                 665                 670         
Glu Pro Ala Ser Gly Glu Thr Glu Ala Ala Pro Thr Phe Gly Glu Val
        675                 680                 685             
Gly Asn Ala Ser Asp Tyr Leu Tyr Pro Ser Gly Leu Gln Arg Ile Thr
    690                 695                 700                 
Lys Phe Ile Tyr Pro Trp Leu Asn Gly Thr Asp Leu Glu Ala Ser Ser
705                 710                 715                 720 
Gly Asp Ala Ser Tyr Gly Gln Asp Ser Ser Asp Tyr Leu Pro Glu Gly
                725                 730                 735     
Ala Thr Asp Gly Ser Ala Gln Pro Ile Leu Pro Ala Gly Gly Gly Pro
            740                 745                 750         
Gly Gly Asn Pro Arg Leu Tyr Asp Glu Leu Ile Arg Val Ser Val Thr
        755                 760                 765             
Ile Lys Asn Thr Gly Lys Val Ala Gly Asp Glu Val Pro Gln Leu Tyr
    770                 775                 780                 
Val Ser Leu Gly Gly Pro Ile Glu Pro Lys Ile Val Leu Arg Gln Phe
785                 790                 795                 800 
Glu Arg Ile Thr Leu Gln Pro Ser Glu Glu Thr Lys Trp Ser Thr Thr
                805                 810                 815     
Leu Thr Arg Arg Asp Leu Ala Asn Trp Asn Val Glu Lys Gln Asp Trp
            820                 825                 830         
Glu Ile Thr Ser Tyr Pro Lys Met Val Phe Val Gly Ser Ser Ser Arg
        835                 840                 845             
Lys Leu Pro Leu Arg Ala Ser Leu Pro Thr Val His
    850                 855                 860 


<210> 11
<211> 2580
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #B, yEdQ893 -> T66I

<400> 11
atgagattta ctttgattga agctgttgct ttgactgctg tttctttggc ttctgctgat 60
gaattggctt attctccacc atattatcca tctccatggg ctaatggtca aggtgattgg 120
gctcaagctt atcaaagagc tgttgatatt gtttctcaaa tgactttgga tgaaaaagtt 180
aatttgacta ctggtattgg ttgggaattg gaattgtgtg ttggtcaaac tggtggtgtt 240
ccaagattgg gtgttccagg tatgtgtttg caagattctc cattgggtgt tagagattct 300
gattataatt ctgcttttcc agctggtatg aatgttgctg ctacttggga taaaaatttg 360
gcttatttga gaggtaaagc tatgggtcaa gaattttctg ataaaggtgc tgatattcaa 420
ttgggtccag ctgctggtcc attgggtaga tctccagatg gtggtagaaa ttgggaaggt 480
ttttctccag atccagcttt gtctggtgtt ttgtttgctg aaactattaa aggtattcaa 540
gatgctggtg ttgttgctac tgctaaacat tatattgctt atgaacaaga acattttaga 600
caagctccag aagctcaagg ttttggtttt aatatttctg aatctggttc tgctaatttg 660
gatgataaaa ctatgcatga attgtatttg tggccatttg ctgatgctat tagagctggt 720
gctggtgctg ttatgtgttc ttataatcaa attaataatt cttatggttg tcaaaattct 780
tatactttga ataaattgtt gaaagctgaa ttgggttttc aaggttttgt tatgtctgat 840
tgggctgctc atcatgctgg tgtttctggt gctttggctg gtttggatat gtctatgcca 900
ggtgatgttg attatgattc tggtacttct tattggggta ctaatttgac tatttctgtt 960
ttgaatggta ctgttccaca atggagagtt gatgatatgg ctgttagaat tatggctgct 1020
tattataaag ttggtagaga tagattgtgg actccaccaa atttttcttc ttggactaga 1080
gatgaatatg gttataaata ttattatgtt tctgaaggtc catatgaaaa agttaatcaa 1140
tatgttaatg ttcaaagaaa tcattctgaa ttgattagaa gaattggtgc tgattctact 1200
gttttgttga aaaatgatgg tgctttgcca ttgactggta aagaaagatt ggttgctttg 1260
attggtgaag atgctggttc taatccatat ggtgctaatg gttgttctga tagaggttgt 1320
gataatggta ctttggctat gggttggggt tctggtactg ctaattttcc atatttggtt 1380
actccagaac aagctatttc taatgaagtt ttgaaacata aaaatggtgt ttttactgct 1440
actgataatt gggctattga tcaaattgaa gctttggcta aaactgcttc tgtttctttg 1500
gtttttgtta atgctgattc tggtgaaggt tatattaatg ttgatggtaa tttgggtgat 1560
agaagaaatt tgactttgtg gagaaatggt gataatgtta ttaaagctgc tgcttctaat 1620
tgtaataata ctattgttgt tattcattct gttggtccag ttttggttaa tgaatggtat 1680
gataatccaa atgttactgc tattttgtgg ggtggtttgc caggtcaaga atctggtaat 1740
tctttggctg atgttttgta tggtagagtt aatccaggtg ctaaatctcc atttacttgg 1800
ggtaaaacta gagaagctta tcaagattat ttggttactg aaccaaataa tggtaatggt 1860
gctccacaag aagattttgt tgaaggtgtt tttattgatt atagaggttt tgataaaaga 1920
aatgaaactc caatttatga atttggttat ggtttgtctt atactacttt taattattct 1980
aatttggaag ttcaagtttt gtctgctcca gcttatgaac cagcttctgg tgaaactgaa 2040
gctgctccaa cttttggtga agttggtaat gcttctgatt atttgtatcc atctggtttg 2100
caaagaatta ctaaatttat ttatccatgg ttgaatggta ctgatttgga agcttcttct 2160
ggtgatgctt cttatggtca agattcttct gattatttgc cagaaggtgc tactgatggt 2220
tctgctcaac caattttgcc agctggtggt ggtccaggtg gtaatccaag attgtatgat 2280
gaattgatta gagtttctgt tactattaaa aatactggta aagttgctgg tgatgaagtt 2340
ccacaattgt atgtttcttt gggtggtcca aatgaaccaa aaattgtttt gagacaattt 2400
gaaagaatta ctttgcaacc atctgaagaa actaaatggt ctactacttt gactagaaga 2460
gatttggcta attggaatgt tgaaaaacaa gattgggaaa ttacttctta tccaaaaatg 2520
gtttttgttg gttcttcttc tagaaaattg ccattgagag cttctttgcc aactgttcat 2580


<210> 12
<211> 860
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #B, yEdQ893 -> T66I

<400> 12
Met Arg Phe Thr Leu Ile Glu Ala Val Ala Leu Thr Ala Val Ser Leu
 1               5                  10                  15      
Ala Ser Ala Asp Glu Leu Ala Tyr Ser Pro Pro Tyr Tyr Pro Ser Pro
            20                  25                  30          
Trp Ala Asn Gly Gln Gly Asp Trp Ala Gln Ala Tyr Gln Arg Ala Val
        35                  40                  45              
Asp Ile Val Ser Gln Met Thr Leu Asp Glu Lys Val Asn Leu Thr Thr
    50                  55                  60                  
Gly Ile Gly Trp Glu Leu Glu Leu Cys Val Gly Gln Thr Gly Gly Val
65                  70                  75                  80  
Pro Arg Leu Gly Val Pro Gly Met Cys Leu Gln Asp Ser Pro Leu Gly
                85                  90                  95      
Val Arg Asp Ser Asp Tyr Asn Ser Ala Phe Pro Ala Gly Met Asn Val
            100                 105                 110         
Ala Ala Thr Trp Asp Lys Asn Leu Ala Tyr Leu Arg Gly Lys Ala Met
        115                 120                 125             
Gly Gln Glu Phe Ser Asp Lys Gly Ala Asp Ile Gln Leu Gly Pro Ala
    130                 135                 140                 
Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn Trp Glu Gly
145                 150                 155                 160 
Phe Ser Pro Asp Pro Ala Leu Ser Gly Val Leu Phe Ala Glu Thr Ile
                165                 170                 175     
Lys Gly Ile Gln Asp Ala Gly Val Val Ala Thr Ala Lys His Tyr Ile
            180                 185                 190         
Ala Tyr Glu Gln Glu His Phe Arg Gln Ala Pro Glu Ala Gln Gly Phe
        195                 200                 205             
Gly Phe Asn Ile Ser Glu Ser Gly Ser Ala Asn Leu Asp Asp Lys Thr
    210                 215                 220                 
Met His Glu Leu Tyr Leu Trp Pro Phe Ala Asp Ala Ile Arg Ala Gly
225                 230                 235                 240 
Ala Gly Ala Val Met Cys Ser Tyr Asn Gln Ile Asn Asn Ser Tyr Gly
                245                 250                 255     
Cys Gln Asn Ser Tyr Thr Leu Asn Lys Leu Leu Lys Ala Glu Leu Gly
            260                 265                 270         
Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Ala Gly Val
        275                 280                 285             
Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp Val Asp
    290                 295                 300                 
Tyr Asp Ser Gly Thr Ser Tyr Trp Gly Thr Asn Leu Thr Ile Ser Val
305                 310                 315                 320 
Leu Asn Gly Thr Val Pro Gln Trp Arg Val Asp Asp Met Ala Val Arg
                325                 330                 335     
Ile Met Ala Ala Tyr Tyr Lys Val Gly Arg Asp Arg Leu Trp Thr Pro
            340                 345                 350         
Pro Asn Phe Ser Ser Trp Thr Arg Asp Glu Tyr Gly Tyr Lys Tyr Tyr
        355                 360                 365             
Tyr Val Ser Glu Gly Pro Tyr Glu Lys Val Asn Gln Tyr Val Asn Val
    370                 375                 380                 
Gln Arg Asn His Ser Glu Leu Ile Arg Arg Ile Gly Ala Asp Ser Thr
385                 390                 395                 400 
Val Leu Leu Lys Asn Asp Gly Ala Leu Pro Leu Thr Gly Lys Glu Arg
                405                 410                 415     
Leu Val Ala Leu Ile Gly Glu Asp Ala Gly Ser Asn Pro Tyr Gly Ala
            420                 425                 430         
Asn Gly Cys Ser Asp Arg Gly Cys Asp Asn Gly Thr Leu Ala Met Gly
        435                 440                 445             
Trp Gly Ser Gly Thr Ala Asn Phe Pro Tyr Leu Val Thr Pro Glu Gln
    450                 455                 460                 
Ala Ile Ser Asn Glu Val Leu Lys His Lys Asn Gly Val Phe Thr Ala
465                 470                 475                 480 
Thr Asp Asn Trp Ala Ile Asp Gln Ile Glu Ala Leu Ala Lys Thr Ala
                485                 490                 495     
Ser Val Ser Leu Val Phe Val Asn Ala Asp Ser Gly Glu Gly Tyr Ile
            500                 505                 510         
Asn Val Asp Gly Asn Leu Gly Asp Arg Arg Asn Leu Thr Leu Trp Arg
        515                 520                 525             
Asn Gly Asp Asn Val Ile Lys Ala Ala Ala Ser Asn Cys Asn Asn Thr
    530                 535                 540                 
Ile Val Val Ile His Ser Val Gly Pro Val Leu Val Asn Glu Trp Tyr
545                 550                 555                 560 
Asp Asn Pro Asn Val Thr Ala Ile Leu Trp Gly Gly Leu Pro Gly Gln
                565                 570                 575     
Glu Ser Gly Asn Ser Leu Ala Asp Val Leu Tyr Gly Arg Val Asn Pro
            580                 585                 590         
Gly Ala Lys Ser Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln
        595                 600                 605             
Asp Tyr Leu Val Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln Glu
    610                 615                 620                 
Asp Phe Val Glu Gly Val Phe Ile Asp Tyr Arg Gly Phe Asp Lys Arg
625                 630                 635                 640 
Asn Glu Thr Pro Ile Tyr Glu Phe Gly Tyr Gly Leu Ser Tyr Thr Thr
                645                 650                 655     
Phe Asn Tyr Ser Asn Leu Glu Val Gln Val Leu Ser Ala Pro Ala Tyr
            660                 665                 670         
Glu Pro Ala Ser Gly Glu Thr Glu Ala Ala Pro Thr Phe Gly Glu Val
        675                 680                 685             
Gly Asn Ala Ser Asp Tyr Leu Tyr Pro Ser Gly Leu Gln Arg Ile Thr
    690                 695                 700                 
Lys Phe Ile Tyr Pro Trp Leu Asn Gly Thr Asp Leu Glu Ala Ser Ser
705                 710                 715                 720 
Gly Asp Ala Ser Tyr Gly Gln Asp Ser Ser Asp Tyr Leu Pro Glu Gly
                725                 730                 735     
Ala Thr Asp Gly Ser Ala Gln Pro Ile Leu Pro Ala Gly Gly Gly Pro
            740                 745                 750         
Gly Gly Asn Pro Arg Leu Tyr Asp Glu Leu Ile Arg Val Ser Val Thr
        755                 760                 765             
Ile Lys Asn Thr Gly Lys Val Ala Gly Asp Glu Val Pro Gln Leu Tyr
    770                 775                 780                 
Val Ser Leu Gly Gly Pro Asn Glu Pro Lys Ile Val Leu Arg Gln Phe
785                 790                 795                 800 
Glu Arg Ile Thr Leu Gln Pro Ser Glu Glu Thr Lys Trp Ser Thr Thr
                805                 810                 815     
Leu Thr Arg Arg Asp Leu Ala Asn Trp Asn Val Glu Lys Gln Asp Trp
            820                 825                 830         
Glu Ile Thr Ser Tyr Pro Lys Met Val Phe Val Gly Ser Ser Ser Arg
        835                 840                 845             
Lys Leu Pro Leu Arg Ala Ser Leu Pro Thr Val His
    850                 855                 860 


<210> 13
<211> 2580
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #16, yEdQ894 -> S247S/Q250L

<400> 13
atgagattta ctttgattga agctgttgct ttgactgctg tttctttggc ttctgctgat 60
gaattggctt attctccacc atattatcca tctccatggg ctaatggtca aggtgattgg 120
gctcaagctt atcaaagagc tgttgatatt gtttctcaaa tgactttgga tgaaaaagtt 180
aatttgacta ctggtactgg ttgggaattg gaattgtgtg ttggtcaaac tggtggtgtt 240
ccaagattgg gtgttccagg tatgtgtttg caagattctc cattgggtgt tagagattct 300
gattataatt ctgcttttcc agctggtatg aatgttgctg ctacttggga taaaaatttg 360
gcttatttga gaggtaaagc tatgggtcaa gaattttctg ataaaggtgc tgatattcaa 420
ttgggtccag ctgctggtcc attgggtaga tctccagatg gtggtagaaa ttgggaaggt 480
ttttctccag atccagcttt gtctggtgtt ttgtttgctg aaactattaa aggtattcaa 540
gatgctggtg ttgttgctac tgctaaacat tatattgctt atgaacaaga acattttaga 600
caagctccag aagctcaagg ttttggtttt aatatttctg aatctggttc tgctaatttg 660
gatgataaaa ctatgcatga attgtatttg tggccatttg ctgatgctat tagagctggt 720
gctggtgctg ttatgtgttc gtataatcta attaataatt cttatggttg tcaaaattct 780
tatactttga ataaattgtt gaaagctgaa ttgggttttc aaggttttgt tatgtctgat 840
tgggctgctc atcatgctgg tgtttctggt gctttggctg gtttggatat gtctatgcca 900
ggtgatgttg attatgattc tggtacttct tattggggta ctaatttgac tatttctgtt 960
ttgaatggta ctgttccaca atggagagtt gatgatatgg ctgttagaat tatggctgct 1020
tattataaag ttggtagaga tagattgtgg actccaccaa atttttcttc ttggactaga 1080
gatgaatatg gttataaata ttattatgtt tctgaaggtc catatgaaaa agttaatcaa 1140
tatgttaatg ttcaaagaaa tcattctgaa ttgattagaa gaattggtgc tgattctact 1200
gttttgttga aaaatgatgg tgctttgcca ttgactggta aagaaagatt ggttgctttg 1260
attggtgaag atgctggttc taatccatat ggtgctaatg gttgttctga tagaggttgt 1320
gataatggta ctttggctat gggttggggt tctggtactg ctaattttcc atatttggtt 1380
actccagaac aagctatttc taatgaagtt ttgaaacata aaaatggtgt ttttactgct 1440
actgataatt gggctattga tcaaattgaa gctttggcta aaactgcttc tgtttctttg 1500
gtttttgtta atgctgattc tggtgaaggt tatattaatg ttgatggtaa tttgggtgat 1560
agaagaaatt tgactttgtg gagaaatggt gataatgtta ttaaagctgc tgcttctaat 1620
tgtaataata ctattgttgt tattcattct gttggtccag ttttggttaa tgaatggtat 1680
gataatccaa atgttactgc tattttgtgg ggtggtttgc caggtcaaga atctggtaat 1740
tctttggctg atgttttgta tggtagagtt aatccaggtg ctaaatctcc atttacttgg 1800
ggtaaaacta gagaagctta tcaagattat ttggttactg aaccaaataa tggtaatggt 1860
gctccacaag aagattttgt tgaaggtgtt tttattgatt atagaggttt tgataaaaga 1920
aatgaaactc caatttatga atttggttat ggtttgtctt atactacttt taattattct 1980
aatttggaag ttcaagtttt gtctgctcca gcttatgaac cagcttctgg tgaaactgaa 2040
gctgctccaa cttttggtga agttggtaat gcttctgatt atttgtatcc atctggtttg 2100
caaagaatta ctaaatttat ttatccatgg ttgaatggta ctgatttgga agcttcttct 2160
ggtgatgctt cttatggtca agattcttct gattatttgc cagaaggtgc tactgatggt 2220
tctgctcaac caattttgcc agctggtggt ggtccaggtg gtaatccaag attgtatgat 2280
gaattgatta gagtttctgt tactattaaa aatactggta aagttgctgg tgatgaagtt 2340
ccacaattgt atgtttcttt gggtggtcca aatgaaccaa aaattgtttt gagacaattt 2400
gaaagaatta ctttgcaacc atctgaagaa actaaatggt ctactacttt gactagaaga 2460
gatttggcta attggaatgt tgaaaaacaa gattgggaaa ttacttctta tccaaaaatg 2520
gtttttgttg gttcttcttc tagaaaattg ccattgagag cttctttgcc aactgttcat 2580


<210> 14
<211> 860
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic mutant beta-glucosidase (BG), BG Orf
      mutant #16, yEdQ894 -> S247S/Q250L

<400> 14
Met Arg Phe Thr Leu Ile Glu Ala Val Ala Leu Thr Ala Val Ser Leu
 1               5                  10                  15      
Ala Ser Ala Asp Glu Leu Ala Tyr Ser Pro Pro Tyr Tyr Pro Ser Pro
            20                  25                  30          
Trp Ala Asn Gly Gln Gly Asp Trp Ala Gln Ala Tyr Gln Arg Ala Val
        35                  40                  45              
Asp Ile Val Ser Gln Met Thr Leu Asp Glu Lys Val Asn Leu Thr Thr
    50                  55                  60                  
Gly Thr Gly Trp Glu Leu Glu Leu Cys Val Gly Gln Thr Gly Gly Val
65                  70                  75                  80  
Pro Arg Leu Gly Val Pro Gly Met Cys Leu Gln Asp Ser Pro Leu Gly
                85                  90                  95      
Val Arg Asp Ser Asp Tyr Asn Ser Ala Phe Pro Ala Gly Met Asn Val
            100                 105                 110         
Ala Ala Thr Trp Asp Lys Asn Leu Ala Tyr Leu Arg Gly Lys Ala Met
        115                 120                 125             
Gly Gln Glu Phe Ser Asp Lys Gly Ala Asp Ile Gln Leu Gly Pro Ala
    130                 135                 140                 
Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn Trp Glu Gly
145                 150                 155                 160 
Phe Ser Pro Asp Pro Ala Leu Ser Gly Val Leu Phe Ala Glu Thr Ile
                165                 170                 175     
Lys Gly Ile Gln Asp Ala Gly Val Val Ala Thr Ala Lys His Tyr Ile
            180                 185                 190         
Ala Tyr Glu Gln Glu His Phe Arg Gln Ala Pro Glu Ala Gln Gly Phe
        195                 200                 205             
Gly Phe Asn Ile Ser Glu Ser Gly Ser Ala Asn Leu Asp Asp Lys Thr
    210                 215                 220                 
Met His Glu Leu Tyr Leu Trp Pro Phe Ala Asp Ala Ile Arg Ala Gly
225                 230                 235                 240 
Ala Gly Ala Val Met Cys Ser Tyr Asn Leu Ile Asn Asn Ser Tyr Gly
                245                 250                 255     
Cys Gln Asn Ser Tyr Thr Leu Asn Lys Leu Leu Lys Ala Glu Leu Gly
            260                 265                 270         
Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Ala Gly Val
        275                 280                 285             
Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp Val Asp
    290                 295                 300                 
Tyr Asp Ser Gly Thr Ser Tyr Trp Gly Thr Asn Leu Thr Ile Ser Val
305                 310                 315                 320 
Leu Asn Gly Thr Val Pro Gln Trp Arg Val Asp Asp Met Ala Val Arg
                325                 330                 335     
Ile Met Ala Ala Tyr Tyr Lys Val Gly Arg Asp Arg Leu Trp Thr Pro
            340                 345                 350         
Pro Asn Phe Ser Ser Trp Thr Arg Asp Glu Tyr Gly Tyr Lys Tyr Tyr
        355                 360                 365             
Tyr Val Ser Glu Gly Pro Tyr Glu Lys Val Asn Gln Tyr Val Asn Val
    370                 375                 380                 
Gln Arg Asn His Ser Glu Leu Ile Arg Arg Ile Gly Ala Asp Ser Thr
385                 390                 395                 400 
Val Leu Leu Lys Asn Asp Gly Ala Leu Pro Leu Thr Gly Lys Glu Arg
                405                 410                 415     
Leu Val Ala Leu Ile Gly Glu Asp Ala Gly Ser Asn Pro Tyr Gly Ala
            420                 425                 430         
Asn Gly Cys Ser Asp Arg Gly Cys Asp Asn Gly Thr Leu Ala Met Gly
        435                 440                 445             
Trp Gly Ser Gly Thr Ala Asn Phe Pro Tyr Leu Val Thr Pro Glu Gln
    450                 455                 460                 
Ala Ile Ser Asn Glu Val Leu Lys His Lys Asn Gly Val Phe Thr Ala
465                 470                 475                 480 
Thr Asp Asn Trp Ala Ile Asp Gln Ile Glu Ala Leu Ala Lys Thr Ala
                485                 490                 495     
Ser Val Ser Leu Val Phe Val Asn Ala Asp Ser Gly Glu Gly Tyr Ile
            500                 505                 510         
Asn Val Asp Gly Asn Leu Gly Asp Arg Arg Asn Leu Thr Leu Trp Arg
        515                 520                 525             
Asn Gly Asp Asn Val Ile Lys Ala Ala Ala Ser Asn Cys Asn Asn Thr
    530                 535                 540                 
Ile Val Val Ile His Ser Val Gly Pro Val Leu Val Asn Glu Trp Tyr
545                 550                 555                 560 
Asp Asn Pro Asn Val Thr Ala Ile Leu Trp Gly Gly Leu Pro Gly Gln
                565                 570                 575     
Glu Ser Gly Asn Ser Leu Ala Asp Val Leu Tyr Gly Arg Val Asn Pro
            580                 585                 590         
Gly Ala Lys Ser Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln
        595                 600                 605             
Asp Tyr Leu Val Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln Glu
    610                 615                 620                 
Asp Phe Val Glu Gly Val Phe Ile Asp Tyr Arg Gly Phe Asp Lys Arg
625                 630                 635                 640 
Asn Glu Thr Pro Ile Tyr Glu Phe Gly Tyr Gly Leu Ser Tyr Thr Thr
                645                 650                 655     
Phe Asn Tyr Ser Asn Leu Glu Val Gln Val Leu Ser Ala Pro Ala Tyr
            660                 665                 670         
Glu Pro Ala Ser Gly Glu Thr Glu Ala Ala Pro Thr Phe Gly Glu Val
        675                 680                 685             
Gly Asn Ala Ser Asp Tyr Leu Tyr Pro Ser Gly Leu Gln Arg Ile Thr
    690                 695                 700                 
Lys Phe Ile Tyr Pro Trp Leu Asn Gly Thr Asp Leu Glu Ala Ser Ser
705                 710                 715                 720 
Gly Asp Ala Ser Tyr Gly Gln Asp Ser Ser Asp Tyr Leu Pro Glu Gly
                725                 730                 735     
Ala Thr Asp Gly Ser Ala Gln Pro Ile Leu Pro Ala Gly Gly Gly Pro
            740                 745                 750         
Gly Gly Asn Pro Arg Leu Tyr Asp Glu Leu Ile Arg Val Ser Val Thr
        755                 760                 765             
Ile Lys Asn Thr Gly Lys Val Ala Gly Asp Glu Val Pro Gln Leu Tyr
    770                 775                 780                 
Val Ser Leu Gly Gly Pro Asn Glu Pro Lys Ile Val Leu Arg Gln Phe
785                 790                 795                 800 
Glu Arg Ile Thr Leu Gln Pro Ser Glu Glu Thr Lys Trp Ser Thr Thr
                805                 810                 815     
Leu Thr Arg Arg Asp Leu Ala Asn Trp Asn Val Glu Lys Gln Asp Trp
            820                 825                 830         
Glu Ile Thr Ser Tyr Pro Lys Met Val Phe Val Gly Ser Ser Ser Arg
        835                 840                 845             
Lys Leu Pro Leu Arg Ala Ser Leu Pro Thr Val His
    850                 855                 860 


<210> 15
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic beta-glucosidase (BG) highly conserved
      nucleophile site

<400> 15
Val Met Ser Asp Trp
 1               5  


<210> 16
<211> 27
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids 58-92
      of wild type beta-glucosidase (BG)

<400> 16
Glu Lys Val Asn Leu Thr Thr Gly Thr Gly Trp Glu Leu Glu Leu Cys
 1               5                  10                  15      
Val Gly Gln Thr Gly Gly Val Pro Arg Leu Gly
            20                  25          


<210> 17
<211> 31
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      127-157 of wild type beta-glucosidase (BG)

<400> 17
Ala Met Gly Gln Glu Phe Ser Asp Lys Gly Ala Asp Ile Gln Leu Gly
 1               5                  10                  15      
Pro Ala Ala Gly Pro Leu Gly Arg Ser Pro Asp Gly Gly Arg Asn
            20                  25                  30      


<210> 18
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      159-180 of wild type beta-glucosidase (BG)

<400> 18
Glu Gly Phe Ser Pro Asp Pro Ala Leu Ser Gly Val Leu Phe Ala Glu
 1               5                  10                  15      
Thr Ile Lys Gly Ile Gln
            20          


<210> 19
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      242-260 of wild type beta-glucosidase (BG)

<400> 19
Gly Ala Val Met Cys Ser Tyr Asn Gln Ile Asn Asn Ser Tyr Gly Cys
 1               5                  10                  15      
Gln Asn Ser
            

<210> 20
<211> 31
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      272-302 of wild type beta-glucosidase (BG)

<400> 20
Gly Phe Gln Gly Phe Val Met Ser Asp Trp Ala Ala His His Ala Gly
 1               5                  10                  15      
Val Ser Gly Ala Leu Ala Gly Leu Asp Met Ser Met Pro Gly Asp
            20                  25                  30      


<210> 21
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      362-379 of wild type beta-glucosidase (BG)

<400> 21
Glu Tyr Gly Tyr Lys Tyr Tyr Tyr Val Ser Glu Gly Pro Tyr Glu Lys
 1               5                  10                  15      
Val Asn
        

<210> 22
<211> 27
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      597-623 of wild type beta-glucosidase (BG)

<400> 22
Pro Phe Thr Trp Gly Lys Thr Arg Glu Ala Tyr Gln Asp Tyr Leu Val
 1               5                  10                  15      
Thr Glu Pro Asn Asn Gly Asn Gly Ala Pro Gln
            20                  25          


<210> 23
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      780-803 of wild type beta-glucosidase (BG)

<400> 23
Val Pro Gln Leu Tyr Val Ser Leu Gly Gly Pro Asn Glu Pro Lys Ile
 1               5                  10                  15      
Val Leu Arg Gln Phe Glu Arg Ile
            20                  


<210> 24
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      272-297 of wild type beta-glucosidase (BG)

<220> 
<221> VARIANT        
<222> (11)...(11)
<223> Xaa = Ala, Thr or Trp

<220> 
<221> VARIANT        
<222> (13)...(13)
<223> Xaa = His or Gln

<220> 
<221> VARIANT        
<222> (15)...(15)
<223> Xaa = Ala, Ser or Thr

<220> 
<221> VARIANT        
<222> (18)...(18)
<223> Xaa = Ser, Gly or Ala

<220> 
<221> VARIANT        
<222> (19)...(19)
<223> Xaa = Gly, Ala or Ser

<220> 
<221> VARIANT        
<222> (21)...(21)
<223> Xaa = Leu or Val

<400> 24
Gly Phe Gln Gly Phe Val Met Ser Asp Trp Xaa Ala Xaa His Xaa Gly
 1               5                  10                  15      
Val Xaa Xaa Ala Xaa Ala Gly Leu Asp Met
            20                  25      


<210> 25
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      272-297 of wild type beta-glucosidase (BG)

<220> 
<221> VARIANT        
<222> (11)...(11)
<223> Xaa = Ala, Thr or Trp

<220> 
<221> VARIANT        
<222> (13)...(13)
<223> Xaa = His or Gln

<220> 
<221> VARIANT        
<222> (15)...(15)
<223> Xaa = Arg, Asn, Cys, Ile, Leu, Lys, Met, Phe, Pro,
      Trp, Tyr, Val, Asp, His, Gly, Gln or Glu

<220> 
<221> VARIANT        
<222> (18)...(18)
<223> Xaa = Ser, Gly or Ala

<220> 
<221> VARIANT        
<222> (19)...(19)
<223> Xaa = Gly, Ala or Ser

<220> 
<221> VARIANT        
<222> (21)...(21)
<223> Xaa = Leu or Val

<400> 25
Gly Phe Gln Gly Phe Val Met Ser Asp Trp Xaa Ala Xaa His Xaa Gly
 1               5                  10                  15      
Val Xaa Xaa Ala Xaa Ala Gly Leu Asp Met
            20                  25      


<210> 26
<211> 26
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic genus Aspergillus beta-glucosidase (BG)
      conserved motif corresponding to amino acids
      272-297 of wild type beta-glucosidase (BG)

<220> 
<221> VARIANT        
<222> (11)...(11)
<223> Xaa - Ala, Thr or Trp

<220> 
<221> VARIANT        
<222> (13)...(13)
<223> Xaa = His or Gln

<220> 
<221> VARIANT        
<222> (18)...(18)
<223> Xaa = Ser, Gly or Ala

<220> 
<221> VARIANT        
<222> (19)...(19)
<223> Xaa = Gly, Ala or Ser

<220> 
<221> VARIANT        
<222> (21)...(21)
<223> Xaa = Leu or Val

<400> 26
Gly Phe Gln Gly Phe Val Met Ser Asp Trp Xaa Ala Xaa His Asp Gly
 1               5                  10                  15      
Val Xaa Xaa Ala Xaa Ala Gly Leu Asp Met
            20                  25      


<210> 27
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BG Orf FWD directed to
      beta-glucosidase (BG) ORF

<400> 27
atgagattta ctttgattga agctg                                       25

<210> 28
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BG Orf REV directed to
      beta-glucosidase (BG) ORF

<400> 28
ctaatgaaca gttggcaaag aag                                         23

<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BG Cat FWD directed to
      beta-glucosidase (BG) nucleophile region

<400> 29
ggccatttgc tgatgctatt                                             20

<210> 30
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BG Cat REV directed to
      beta-glucosidase (BG) nucleophile region

<400> 30
caactctcca ttgtggaaca g                                           21

<210> 31
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BG Acid-Base FWD directed to
      beta-glucosidase (BG) enzyme substrate hydrolysis region

<400> 31
ctgttccaca atggagagtt g                                           21

<210> 32
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BG Orf REV directed to
      beta-glucosidase (BG) enzyme substrate hydrolysis region

<400> 32
ctaatgaaca gttggcaaag aag                                         23

<210> 33
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer EDQ.JHV.054 used for integration
      into plasmid

<400> 33
agcgatagac ctttggtccc cggatcccca atgagattta ctttgattga agctg      55

<210> 34
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer EDQ.JHV.095 used for integration
      into plasmid

<400> 34
gacggtatcg ataagcttga tatcgaattc ctaatgaaca gttggcaaag aag        53

<210> 35
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer Tau3up F1 used for integration
      into genome

<400> 35
gatcaagatc gctgcgttgt tgttgatgg                                   29

<210> 36
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer Tau3down R1 used for integration
      into genome

<400> 36
gatcttgatc gagcccgtaa tacaacagtg ag                               32


