                         SEQUENCE LISTING

<110>  Brune, Ellen M.
       Beitle, Robert R.
       Ataai, Mohammad M.
       Bartlow, Patrick R.
       Henry, Ralph L.
 
<120>  E. COLI SEPARATOME-BASED PROTEIN EXPRESSION AND PURIFICATION 
       PLATFORM

<130>  BMB0002-401-PC

<150>  61/878,882
<151>  2013-09-17

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  1

His His His His His His 
1               5       


<210>  2
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  2
gccaagcttg tggcatcatc atccgcatat gagtaaagga gaagaacttt tc               52


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
ttggaattca ttatttgtag agct                                              24


<210>  4
<211>  120
<212>  DNA
<213>  Escherichia coli

<400>  4
gcaaaatttc gggaaggcgt ctcgaagaat ttaacggagg gtaaaaaaac cgacgcacac       60

gtgttgctgt gggctgcgac gatatgccca gaccatcatg atcacacccg cgacaatcat      120


<210>  5
<211>  98
<212>  DNA
<213>  Escherichia coli

<400>  5
tttgttgaat ttttattaaa tctgggttga gcgtgtcggg agcaagtgct ggggtatgac       60

gcggactgat tcacaaatct gtcacttttc cttacaac                               98


<210>  6
<211>  96
<212>  DNA
<213>  Escherichia coli

<400>  6
ggcgtactct gacaccgacg aattttaccc agttgcagga ggcacacgcg caacgctaaa       60

caggtaaatt aatattattt ataaacccat aattac                                 96


<210>  7
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  7
cgctggttta aaacgttgga ctgtttttct gacgtagtgg agaaaaacca cctttgaacg       60

ttgattaata ttaataatga gggaaattta atgagct                                97


<210>  8
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  8
gtggagtgac gaaaatcttc atcagagatg acaacggagg aaccgagaag aaaaaagtgg       60

cagagtgatc aataccctct ttaaaagaag agggtta                                97


<210>  9
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  9
taaaacccgt attattgcgc gctttccgta cgactaaagt gattttcgca gcattctggg       60

caaaataaaa tcaaatagcc tacgcaatgt aggctta                                97


<210>  10
<211>  98
<212>  DNA
<213>  Escherichia coli

<400>  10
gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag cacttgctcc       60

cgacacgctc aacccagatt taataaaaat tcaacaaa                               98


<210>  11
<211>  98
<212>  DNA
<213>  Escherichia coli

<400>  11
tttgttgaat ttttattaaa tctgggttga gcgtgtcggg agcaagtgct ggggtatgac       60

gcggactgat tcacaaatct gtcacttttc cttacaac                               98


<210>  12
<211>  96
<212>  DNA
<213>  Escherichia coli

<400>  12
gtaattatgg gtttataaat aatattaatt tacctgttta gcgttgcgcg tgtgcctcct       60

gcaactgggt aaaattcgtc ggtgtcagag tacgcc                                 96


<210>  13
<211>  96
<212>  DNA
<213>  Escherichia coli

<400>  13
ggcgtactct gacaccgacg aattttaccc agttgcagga ggcacacgcg caacgctaaa       60

caggtaaatt aatattattt ataaacccat aattac                                 96


<210>  14
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  14
agctcattaa atttccctca ttattaatat taatcaacgt tcaaaggtgg tttttctcca       60

ctacgtcaga aaaacagtcc aacgttttaa accagcg                                97


<210>  15
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  15
cgctggttta aaacgttgga ctgtttttct gacgtagtgg agaaaaacca cctttgaacg       60

ttgattaata ttaataatga gggaaattta atgagct                                97


<210>  16
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  16
taaccctctt cttttaaaga gggtattgat cactctgcca cttttttctt ctcggttcct       60

ccgttgtcat ctctgatgaa gattttcgtc actccac                                97


<210>  17
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  17
gtggagtgac gaaaatcttc atcagagatg acaacggagg aaccgagaag aaaaaagtgg       60

cagagtgatc aataccctct ttaaaagaag agggtta                                97


<210>  18
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  18
taagcctaca ttgcgtaggc tatttgattt tattttgccc agaatgctgc gaaaatcact       60

ttagtcgtac ggaaagcgcg caataatacg ggtttta                                97


<210>  19
<211>  97
<212>  DNA
<213>  Escherichia coli

<400>  19
taaaacccgt attattgcgc gctttccgta cgactaaagt gattttcgca gcattctggg       60

caaaataaaa tcaaatagcc tacgcaatgt aggctta                                97


<210>  20
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
tttgttgaat ttttattaaa tctgggttga gcgtgtcggg agcaagtgcg ccacccatca       60

cagcttta                                                                68


<210>  21
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag gggaaggcgt       60

ctcgaagaat                                                              70


<210>  22
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
ggcgtactct gacaccgacg aattttaccc agttgcagga ggcacacgcc acccatcaca       60

gcttta                                                                  66


<210>  23
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
gtaattatgg gtttataaat aatattaatt tacctgttta gcgttgcgcg gggaaggcgt       60

ctcgaagaat                                                              70


<210>  24
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
cgctggttta aaacgttgga ctgtttttct gacgtagtgg agaaaaacgc cacccatcac       60

agcttta                                                                 67


<210>  25
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
agctcattaa atttccctca ttattaatat taatcaacgt tcaaaggtgg gggaaggcgt       60

ctcgaagaat                                                              70


<210>  26
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
gtggagtgac gaaaatcttc atcagagatg acaacggagg aaccgagcgc cacccatcac       60

agcttta                                                                 67


<210>  27
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
taaccctctt cttttaaaga gggtattgat cactctgcca cttttttctt gggaaggcgt       60

ctcgaagaat                                                              70


<210>  28
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
taaaacccgt attattgcgc gctttccgta cgactaaagt gattttccgc cacccatcac       60

agcttta                                                                 67


<210>  29
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
taagcctaca ttgcgtaggc tatttgattt tattttgccc agaatgctgc gggaaggcgt       60

ctcgaagaat                                                              70


<210>  30
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg gtgtaggctg       60

gagctgcttc                                                              70


<210>  31
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
ccggtgccat cacatcgatt atcgcctggg gatagcgcgc ctggagcgtg atgggaatta       60

gccatggtcc                                                              70


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
gcaaaaccaa catccgccat                                                   20


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
ccggtgccat cacatcgatt a                                                 21


<210>  34
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
cggcatcatt gctgtgtaaa ctgggtttta acgccgttca tcatccggca gtgtaggctg       60

gagctgcttc                                                              70


<210>  35
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct atgggaatta       60

gccatggtcc                                                              70


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
gcggcatcat tgctgtgtaa                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
gaagacggtg atgggttcgt                                                   20


<210>  38
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
cgtactgtca agggagcgtt actgactaac ctgctgtttg ttttagggat gtgtaggctg       60

gagctgcttc                                                              70


<210>  39
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
gagctggagc gtgagaacaa ccatctgaaa gaacagcaga acggctggca atgggaatta       60

gccatggtcc                                                              70


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
cgtactgtca agggagcgtt                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
aagagctgga gcgtgagaac                                                   20


<210>  42
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  42
cgactaacat ccttcccccg tccgttgtat agtgacctct ctcttgcggt gtgtaggctg       60

gagctgcttc                                                              70


<210>  43
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  43
aaagcaaagg cttgatccgc ggggacaaat tgtgaacgtc ccggcgcgtc atgggaatta       60

gccatggtcc                                                              70


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  44
cgactaacat ccttcccccg                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
aaagcaaagg cttgatccgc                                                   20


<210>  46
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
ttggagcgtc agcattcact gctggaaaat ccatgtgctt atgggttgtt gtgtaggctg       60

gagctgcttc                                                              70


<210>  47
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  47
tattgcagga gctgcgtagg cctgataagc gtagcgcatc aggcagtttg atgggaatta       60

gccatggtcc                                                              70


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  48
cgtttcgcac taatctgccg                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  49
tattgcagga gctgcgtagg                                                   20


<210>  50
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  50
ttggagcgtc agcattcact gctggaaaat ccatgtgctt atgggttgtt gtgtaggctg       60

gagctgcttc                                                              70


<210>  51
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  51
cgacgaggaa gggttggatt tgtcacaata aattgtggcg gattatcacc atgggaatta       60

gccatggtcc                                                              70


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  52
ttggagcgtc agcattcact                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  53
cgacgaggaa gggttggatt                                                   20


