                         SEQUENCE LISTING

<110>  Bayer CropScience NV
       The University Court of the University of Edinburgh
       Meulewaeter, Frank
       Brande, Ilse  Van den
       Fry, Stephen C
       Mohler, Kyle E
       Frankova, Lenka
       Simmons, Tom J
       Holland, Claire
       Hudson, Andrew
 
<120>  Novel hetero-transglycosylase and uses thereof

<130>  BCS13-2019

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  843
<212>  DNA
<213>  Equisetum fluviatile


<220>
<221>  CDS
<222>  (1)..(843)

<220>
<221>  sig_peptide
<222>  (1)..(63)

<400>  1
atg ctg ggt ctg gtg ttt gga atg ttg gtg atc atg ctg gcg tct cca         48
Met Leu Gly Leu Val Phe Gly Met Leu Val Ile Met Leu Ala Ser Pro           
1               5                   10                  15                

aaa tta gca atg gca ggt ttc tat ggg gac ttt cag gta gaa ccg gtt         96
Lys Leu Ala Met Ala Gly Phe Tyr Gly Asp Phe Gln Val Glu Pro Val           
            20                  25                  30                    

ccc gac cac gtg ata atc caa agc gat agc ctc ctc caa ctc acc atg        144
Pro Asp His Val Ile Ile Gln Ser Asp Ser Leu Leu Gln Leu Thr Met           
        35                  40                  45                        

gat aag aac tct ggt ggc tca gtt gtc tcc aaa agt aat tat ctg ttt        192
Asp Lys Asn Ser Gly Gly Ser Val Val Ser Lys Ser Asn Tyr Leu Phe           
    50                  55                  60                            

ggc tac ttc aac atg aag atg aag ctc ata tca gga aac tct gca ggg        240
Gly Tyr Phe Asn Met Lys Met Lys Leu Ile Ser Gly Asn Ser Ala Gly           
65                  70                  75                  80            

aca gta acc aca ttc tat atc ttc tct gat gaa gca aac cac gat gag        288
Thr Val Thr Thr Phe Tyr Ile Phe Ser Asp Glu Ala Asn His Asp Glu           
                85                  90                  95                

ata gac ttt gag ttc ctt ggc aac tat tca ggg gat cct tat ctt ttg        336
Ile Asp Phe Glu Phe Leu Gly Asn Tyr Ser Gly Asp Pro Tyr Leu Leu           
            100                 105                 110                   

cat act aat att ttt gca agt ggt gtt gga aat aga gaa caa caa ttt        384
His Thr Asn Ile Phe Ala Ser Gly Val Gly Asn Arg Glu Gln Gln Phe           
        115                 120                 125                       

ttt ctg tgg ttt gac cct aca gct gac ttc cat gat tat aca ata att        432
Phe Leu Trp Phe Asp Pro Thr Ala Asp Phe His Asp Tyr Thr Ile Ile           
    130                 135                 140                           

tgg aac cct caa caa ata ttg ttt ctt gtt gat gga agg gct gtt aga        480
Trp Asn Pro Gln Gln Ile Leu Phe Leu Val Asp Gly Arg Ala Val Arg           
145                 150                 155                 160           

tct ttt ccg aat aat gag gct ata ggt gtc cct tac tta aaa agt caa        528
Ser Phe Pro Asn Asn Glu Ala Ile Gly Val Pro Tyr Leu Lys Ser Gln           
                165                 170                 175               

tgg atg aat gta cat tta agt ctt tgg aat ggc gag act tgg gcc aca        576
Trp Met Asn Val His Leu Ser Leu Trp Asn Gly Glu Thr Trp Ala Thr           
            180                 185                 190                   

cta gga ggg ttg aga agg ata gat tgg aat tca gcc cct ttt gta gct        624
Leu Gly Gly Leu Arg Arg Ile Asp Trp Asn Ser Ala Pro Phe Val Ala           
        195                 200                 205                       

tcc tat tct act ttt gta gga gac tca tgc ttc gat agc gca gat tcc        672
Ser Tyr Ser Thr Phe Val Gly Asp Ser Cys Phe Asp Ser Ala Asp Ser           
    210                 215                 220                           

ccg tgc atg gcc tca aaa tgg tgg aac caa gct gca tat caa tct tta        720
Pro Cys Met Ala Ser Lys Trp Trp Asn Gln Ala Ala Tyr Gln Ser Leu           
225                 230                 235                 240           

agc aca agt gat gcc agc agt att caa tgg gtt agg gaa aat tat ctc        768
Ser Thr Ser Asp Ala Ser Ser Ile Gln Trp Val Arg Glu Asn Tyr Leu           
                245                 250                 255               

aaa tat gac tat tgt tat gat aca aaa ctc tat ccg aac ggc ttt ccc        816
Lys Tyr Asp Tyr Cys Tyr Asp Thr Lys Leu Tyr Pro Asn Gly Phe Pro           
            260                 265                 270                   

aga gaa tgc tca aac cgt ggt ttc tag                                    843
Arg Glu Cys Ser Asn Arg Gly Phe                                           
        275                 280                                           


<210>  2
<211>  280
<212>  PRT
<213>  Equisetum fluviatile

<400>  2

Met Leu Gly Leu Val Phe Gly Met Leu Val Ile Met Leu Ala Ser Pro 
1               5                   10                  15      


Lys Leu Ala Met Ala Gly Phe Tyr Gly Asp Phe Gln Val Glu Pro Val 
            20                  25                  30          


Pro Asp His Val Ile Ile Gln Ser Asp Ser Leu Leu Gln Leu Thr Met 
        35                  40                  45              


Asp Lys Asn Ser Gly Gly Ser Val Val Ser Lys Ser Asn Tyr Leu Phe 
    50                  55                  60                  


Gly Tyr Phe Asn Met Lys Met Lys Leu Ile Ser Gly Asn Ser Ala Gly 
65                  70                  75                  80  


Thr Val Thr Thr Phe Tyr Ile Phe Ser Asp Glu Ala Asn His Asp Glu 
                85                  90                  95      


Ile Asp Phe Glu Phe Leu Gly Asn Tyr Ser Gly Asp Pro Tyr Leu Leu 
            100                 105                 110         


His Thr Asn Ile Phe Ala Ser Gly Val Gly Asn Arg Glu Gln Gln Phe 
        115                 120                 125             


Phe Leu Trp Phe Asp Pro Thr Ala Asp Phe His Asp Tyr Thr Ile Ile 
    130                 135                 140                 


Trp Asn Pro Gln Gln Ile Leu Phe Leu Val Asp Gly Arg Ala Val Arg 
145                 150                 155                 160 


Ser Phe Pro Asn Asn Glu Ala Ile Gly Val Pro Tyr Leu Lys Ser Gln 
                165                 170                 175     


Trp Met Asn Val His Leu Ser Leu Trp Asn Gly Glu Thr Trp Ala Thr 
            180                 185                 190         


Leu Gly Gly Leu Arg Arg Ile Asp Trp Asn Ser Ala Pro Phe Val Ala 
        195                 200                 205             


Ser Tyr Ser Thr Phe Val Gly Asp Ser Cys Phe Asp Ser Ala Asp Ser 
    210                 215                 220                 


Pro Cys Met Ala Ser Lys Trp Trp Asn Gln Ala Ala Tyr Gln Ser Leu 
225                 230                 235                 240 


Ser Thr Ser Asp Ala Ser Ser Ile Gln Trp Val Arg Glu Asn Tyr Leu 
                245                 250                 255     


Lys Tyr Asp Tyr Cys Tyr Asp Thr Lys Leu Tyr Pro Asn Gly Phe Pro 
            260                 265                 270         


Arg Glu Cys Ser Asn Arg Gly Phe 
        275                 280 


<210>  3
<211>  1122
<212>  DNA
<213>  artificial

<220>
<223>  coding sequence CXE fusion protein for expression in Pichia


<220>
<221>  CDS
<222>  (1)..(1122)

<220>
<221>  alpha factor signal sequence
<222>  (1)..(267)

<220>
<221>  HTG
<222>  (274)..(1050)

<220>
<221>  c-myc epitope
<222>  (1057)..(1086)

<220>
<221>  polyhistidine tag
<222>  (1102)..(1119)

<400>  3
atg aga ttt cct tca att ttt act gct gtt tta ttc gca gca tcc tcc         48
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser           
1               5                   10                  15                

gca tta gct gct cca gtc aac act aca aca gaa gat gaa acg gca caa         96
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln           
            20                  25                  30                    

att ccg gct gaa gct gtc atc ggt tac tca gat tta gaa ggg gat ttc        144
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe           
        35                  40                  45                        

gat gtt gct gtt ttg cca ttt tcc aac agc aca aat aac ggg tta ttg        192
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu           
    50                  55                  60                            

ttt ata aat act act att gcc agc att gct gct aaa gaa gaa ggg gta        240
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val           
65                  70                  75                  80            

tct ctc gag aaa aga gag gct gaa gct gaa ttc ggt ttc tat ggg gac        288
Ser Leu Glu Lys Arg Glu Ala Glu Ala Glu Phe Gly Phe Tyr Gly Asp           
                85                  90                  95                

ttt cag gta gaa ccg gtt ccc gac cac gtg ata atc caa agc gat agc        336
Phe Gln Val Glu Pro Val Pro Asp His Val Ile Ile Gln Ser Asp Ser           
            100                 105                 110                   

ctc ctc caa ctc acc atg gat aag aac tct ggt ggc tca gtt gtc tcc        384
Leu Leu Gln Leu Thr Met Asp Lys Asn Ser Gly Gly Ser Val Val Ser           
        115                 120                 125                       

aaa agt aat tat ctg ttt ggc tac ttc aac atg aag atg aag ctc ata        432
Lys Ser Asn Tyr Leu Phe Gly Tyr Phe Asn Met Lys Met Lys Leu Ile           
    130                 135                 140                           

tca gga aac tct gca ggg aca gta acc aca ttc tat atc ttc tct gat        480
Ser Gly Asn Ser Ala Gly Thr Val Thr Thr Phe Tyr Ile Phe Ser Asp           
145                 150                 155                 160           

gaa gca aac cac gat gag ata gac ttt gag ttc ctt ggc aac tat tca        528
Glu Ala Asn His Asp Glu Ile Asp Phe Glu Phe Leu Gly Asn Tyr Ser           
                165                 170                 175               

ggg gat cct tat ctt ttg cat act aat att ttt gca agt ggt gtt gga        576
Gly Asp Pro Tyr Leu Leu His Thr Asn Ile Phe Ala Ser Gly Val Gly           
            180                 185                 190                   

aat aga gaa caa caa ttt ttt ctg tgg ttt gac cct aca gct gac ttc        624
Asn Arg Glu Gln Gln Phe Phe Leu Trp Phe Asp Pro Thr Ala Asp Phe           
        195                 200                 205                       

cat gat tat aca ata att tgg aac cct caa caa ata ttg ttt ctt gtt        672
His Asp Tyr Thr Ile Ile Trp Asn Pro Gln Gln Ile Leu Phe Leu Val           
    210                 215                 220                           

gat gga agg gct gtt aga tct ttt ccg aat aat gag gct ata ggt gtc        720
Asp Gly Arg Ala Val Arg Ser Phe Pro Asn Asn Glu Ala Ile Gly Val           
225                 230                 235                 240           

cct tac tta aaa agt caa tgg atg aat gta cat tta agt ctt tgg aat        768
Pro Tyr Leu Lys Ser Gln Trp Met Asn Val His Leu Ser Leu Trp Asn           
                245                 250                 255               

ggc gag act tgg gcc aca cta gga ggg ttg aga agg ata gat tgg aat        816
Gly Glu Thr Trp Ala Thr Leu Gly Gly Leu Arg Arg Ile Asp Trp Asn           
            260                 265                 270                   

tca gcc cct ttt gta gct tcc tat tct act ttt gta gga gac tca tgc        864
Ser Ala Pro Phe Val Ala Ser Tyr Ser Thr Phe Val Gly Asp Ser Cys           
        275                 280                 285                       

ttc gat agc gca gat tcc ccg tgc atg gcc tca aaa tgg tgg aac caa        912
Phe Asp Ser Ala Asp Ser Pro Cys Met Ala Ser Lys Trp Trp Asn Gln           
    290                 295                 300                           

gct gca tat caa tct tta agc aca agt gat gcc agc agt att caa tgg        960
Ala Ala Tyr Gln Ser Leu Ser Thr Ser Asp Ala Ser Ser Ile Gln Trp           
305                 310                 315                 320           

gtt agg gaa aat tat ctc aaa tat gac tat tgt tat gat aca aaa ctc       1008
Val Arg Glu Asn Tyr Leu Lys Tyr Asp Tyr Cys Tyr Asp Thr Lys Leu           
                325                 330                 335               

tat ccg aac ggc ttt ccc aga gaa tgc tca aac cgt ggt ttc tat cta       1056
Tyr Pro Asn Gly Phe Pro Arg Glu Cys Ser Asn Arg Gly Phe Tyr Leu           
            340                 345                 350                   

gaa caa aaa ctc atc tca gaa gag gat ctg aat agc gcc gtc gac cat       1104
Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His           
        355                 360                 365                       

cat cat cat cat cat tga                                               1122
His His His His His                                                       
    370                                                                   


<210>  4
<211>  373
<212>  PRT
<213>  artificial

<220>
<223>  Synthetic Construct

<400>  4

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg Glu Ala Glu Ala Glu Phe Gly Phe Tyr Gly Asp 
                85                  90                  95      


Phe Gln Val Glu Pro Val Pro Asp His Val Ile Ile Gln Ser Asp Ser 
            100                 105                 110         


Leu Leu Gln Leu Thr Met Asp Lys Asn Ser Gly Gly Ser Val Val Ser 
        115                 120                 125             


Lys Ser Asn Tyr Leu Phe Gly Tyr Phe Asn Met Lys Met Lys Leu Ile 
    130                 135                 140                 


Ser Gly Asn Ser Ala Gly Thr Val Thr Thr Phe Tyr Ile Phe Ser Asp 
145                 150                 155                 160 


Glu Ala Asn His Asp Glu Ile Asp Phe Glu Phe Leu Gly Asn Tyr Ser 
                165                 170                 175     


Gly Asp Pro Tyr Leu Leu His Thr Asn Ile Phe Ala Ser Gly Val Gly 
            180                 185                 190         


Asn Arg Glu Gln Gln Phe Phe Leu Trp Phe Asp Pro Thr Ala Asp Phe 
        195                 200                 205             


His Asp Tyr Thr Ile Ile Trp Asn Pro Gln Gln Ile Leu Phe Leu Val 
    210                 215                 220                 


Asp Gly Arg Ala Val Arg Ser Phe Pro Asn Asn Glu Ala Ile Gly Val 
225                 230                 235                 240 


Pro Tyr Leu Lys Ser Gln Trp Met Asn Val His Leu Ser Leu Trp Asn 
                245                 250                 255     


Gly Glu Thr Trp Ala Thr Leu Gly Gly Leu Arg Arg Ile Asp Trp Asn 
            260                 265                 270         


Ser Ala Pro Phe Val Ala Ser Tyr Ser Thr Phe Val Gly Asp Ser Cys 
        275                 280                 285             


Phe Asp Ser Ala Asp Ser Pro Cys Met Ala Ser Lys Trp Trp Asn Gln 
    290                 295                 300                 


Ala Ala Tyr Gln Ser Leu Ser Thr Ser Asp Ala Ser Ser Ile Gln Trp 
305                 310                 315                 320 


Val Arg Glu Asn Tyr Leu Lys Tyr Asp Tyr Cys Tyr Asp Thr Lys Leu 
                325                 330                 335     


Tyr Pro Asn Gly Phe Pro Arg Glu Cys Ser Asn Arg Gly Phe Tyr Leu 
            340                 345                 350         


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala Val Asp His 
        355                 360                 365             


His His His His His 
    370             


<210>  5
<211>  852
<212>  DNA
<213>  Equisetum hyemale


<220>
<221>  sig_peptide
<222>  (1)..(75)

<220>
<221>  CDS
<222>  (1)..(852)

<400>  5
atg aag aag aag att gga atg gtg ctg ctt ttg ggg ctt ttc atg atc         48
Met Lys Lys Lys Ile Gly Met Val Leu Leu Leu Gly Leu Phe Met Ile           
1               5                   10                  15                

atc ata gcg tct ccc aaa gca gag gca aat ttc tat caa gat ttc gtc         96
Ile Ile Ala Ser Pro Lys Ala Glu Ala Asn Phe Tyr Gln Asp Phe Val           
            20                  25                  30                    

gta gtt aca gct cct gac cat gtc caa atc ctt aat gat aac ctc ctc        144
Val Val Thr Ala Pro Asp His Val Gln Ile Leu Asn Asp Asn Leu Leu           
        35                  40                  45                        

cag ctt acc atg gat aag aat act ggt agc tca att agc tcc acc agt        192
Gln Leu Thr Met Asp Lys Asn Thr Gly Ser Ser Ile Ser Ser Thr Ser           
    50                  55                  60                            

aaa tac ctg ttt ggc tac ttc aac atg agg atg aag ctc ata gca ggc        240
Lys Tyr Leu Phe Gly Tyr Phe Asn Met Arg Met Lys Leu Ile Ala Gly           
65                  70                  75                  80            

aac tct gca ggg aca gtg acc acc ttc tat ctc ttc tcc agt gaa ccc        288
Asn Ser Ala Gly Thr Val Thr Thr Phe Tyr Leu Phe Ser Ser Glu Pro           
                85                  90                  95                

aac cat gat gag cta gac ttt gag ttc ctt ggc aat ctt tca ggg gaa        336
Asn His Asp Glu Leu Asp Phe Glu Phe Leu Gly Asn Leu Ser Gly Glu           
            100                 105                 110                   

cct tat gtt ttg cat aca aat gtt ttt gca agt ggt gtt gga aat aga        384
Pro Tyr Val Leu His Thr Asn Val Phe Ala Ser Gly Val Gly Asn Arg           
        115                 120                 125                       

gaa caa caa ttt ttt ctg tgg ttt gac cct aca act gac ttc cac gac        432
Glu Gln Gln Phe Phe Leu Trp Phe Asp Pro Thr Thr Asp Phe His Asp           
    130                 135                 140                           

tat aca ata att tgg aac cct caa caa gta ttg ttt gtt gtt gat gga        480
Tyr Thr Ile Ile Trp Asn Pro Gln Gln Val Leu Phe Val Val Asp Gly           
145                 150                 155                 160           

agg act gtt aga tct ttc cca aat aat gag gct ata ggt gtc cct tac        528
Arg Thr Val Arg Ser Phe Pro Asn Asn Glu Ala Ile Gly Val Pro Tyr           
                165                 170                 175               

tta aaa agt caa tgg atg aat gta tat gca agc ctt tgg aat ggt gag        576
Leu Lys Ser Gln Trp Met Asn Val Tyr Ala Ser Leu Trp Asn Gly Glu           
            180                 185                 190                   

tct tgg gcc aca cta gga ggg ctg ata aag ata gat tgg agt gta tcc        624
Ser Trp Ala Thr Leu Gly Gly Leu Ile Lys Ile Asp Trp Ser Val Ser           
        195                 200                 205                       

cct ttt gtg gct tcc tat gct gat ttt gca gca gac tca tgc ttt gat        672
Pro Phe Val Ala Ser Tyr Ala Asp Phe Ala Ala Asp Ser Cys Phe Asp           
    210                 215                 220                           

agt gca gat tcc tca tgc atg gcc aca aag tgg tgg aac caa cct gca        720
Ser Ala Asp Ser Ser Cys Met Ala Thr Lys Trp Trp Asn Gln Pro Ala           
225                 230                 235                 240           

tat caa ttt tta agc aca aat gat gca agc agt att caa tgg gtt agg        768
Tyr Gln Phe Leu Ser Thr Asn Asp Ala Ser Ser Ile Gln Trp Val Arg           
                245                 250                 255               

gca aat tat ctc aaa tac gac tat tgt tat gat acg gaa ctc tat cca        816
Ala Asn Tyr Leu Lys Tyr Asp Tyr Cys Tyr Asp Thr Glu Leu Tyr Pro           
            260                 265                 270                   

act cct ccc atc gaa tgt cag aac cgt ggc ttc tag                        852
Thr Pro Pro Ile Glu Cys Gln Asn Arg Gly Phe                               
        275                 280                                           


<210>  6
<211>  283
<212>  PRT
<213>  Equisetum hyemale

<400>  6

Met Lys Lys Lys Ile Gly Met Val Leu Leu Leu Gly Leu Phe Met Ile 
1               5                   10                  15      


Ile Ile Ala Ser Pro Lys Ala Glu Ala Asn Phe Tyr Gln Asp Phe Val 
            20                  25                  30          


Val Val Thr Ala Pro Asp His Val Gln Ile Leu Asn Asp Asn Leu Leu 
        35                  40                  45              


Gln Leu Thr Met Asp Lys Asn Thr Gly Ser Ser Ile Ser Ser Thr Ser 
    50                  55                  60                  


Lys Tyr Leu Phe Gly Tyr Phe Asn Met Arg Met Lys Leu Ile Ala Gly 
65                  70                  75                  80  


Asn Ser Ala Gly Thr Val Thr Thr Phe Tyr Leu Phe Ser Ser Glu Pro 
                85                  90                  95      


Asn His Asp Glu Leu Asp Phe Glu Phe Leu Gly Asn Leu Ser Gly Glu 
            100                 105                 110         


Pro Tyr Val Leu His Thr Asn Val Phe Ala Ser Gly Val Gly Asn Arg 
        115                 120                 125             


Glu Gln Gln Phe Phe Leu Trp Phe Asp Pro Thr Thr Asp Phe His Asp 
    130                 135                 140                 


Tyr Thr Ile Ile Trp Asn Pro Gln Gln Val Leu Phe Val Val Asp Gly 
145                 150                 155                 160 


Arg Thr Val Arg Ser Phe Pro Asn Asn Glu Ala Ile Gly Val Pro Tyr 
                165                 170                 175     


Leu Lys Ser Gln Trp Met Asn Val Tyr Ala Ser Leu Trp Asn Gly Glu 
            180                 185                 190         


Ser Trp Ala Thr Leu Gly Gly Leu Ile Lys Ile Asp Trp Ser Val Ser 
        195                 200                 205             


Pro Phe Val Ala Ser Tyr Ala Asp Phe Ala Ala Asp Ser Cys Phe Asp 
    210                 215                 220                 


Ser Ala Asp Ser Ser Cys Met Ala Thr Lys Trp Trp Asn Gln Pro Ala 
225                 230                 235                 240 


Tyr Gln Phe Leu Ser Thr Asn Asp Ala Ser Ser Ile Gln Trp Val Arg 
                245                 250                 255     


Ala Asn Tyr Leu Lys Tyr Asp Tyr Cys Tyr Asp Thr Glu Leu Tyr Pro 
            260                 265                 270         


Thr Pro Pro Ile Glu Cys Gln Asn Arg Gly Phe 
        275                 280             


<210>  7
<211>  864
<212>  DNA
<213>  Equisetum diffusum


<220>
<221>  sig_peptide
<222>  (1)..(84)

<220>
<221>  CDS
<222>  (1)..(864)

<400>  7
atg aag aag aag acc gcg tcg atg ctg ggt ttg gcg ttt ggg atg ttg         48
Met Lys Lys Lys Thr Ala Ser Met Leu Gly Leu Ala Phe Gly Met Leu           
1               5                   10                  15                

ttg atc atg ctg gcg tct cca aaa tta gca ata gca ggt ttc tat gag         96
Leu Ile Met Leu Ala Ser Pro Lys Leu Ala Ile Ala Gly Phe Tyr Glu           
            20                  25                  30                    

gac ttt gac gta gat cca cct ccc gac cac gtg ata atc caa agt gat        144
Asp Phe Asp Val Asp Pro Pro Pro Asp His Val Ile Ile Gln Ser Asp           
        35                  40                  45                        

agc ctc ctc gaa ctc acc atg gat aag aac tct ggt agc aca gtt gtc        192
Ser Leu Leu Glu Leu Thr Met Asp Lys Asn Ser Gly Ser Thr Val Val           
    50                  55                  60                            

tcc acc cgt aaa tat ctg ttt ggc tac ttc aac atg aag atg aag ctc        240
Ser Thr Arg Lys Tyr Leu Phe Gly Tyr Phe Asn Met Lys Met Lys Leu           
65                  70                  75                  80            

ata tca ggc aac tct gca ggg aca gta acc aca ttc tat atc ttc tct        288
Ile Ser Gly Asn Ser Ala Gly Thr Val Thr Thr Phe Tyr Ile Phe Ser           
                85                  90                  95                

gag gaa gca aac cac gat gag ata gac ttt gag ttc ctt ggc aac tat        336
Glu Glu Ala Asn His Asp Glu Ile Asp Phe Glu Phe Leu Gly Asn Tyr           
            100                 105                 110                   

tca ggg gat cct tat ctt ttg cat act aat att ttt gca agt ggt gtt        384
Ser Gly Asp Pro Tyr Leu Leu His Thr Asn Ile Phe Ala Ser Gly Val           
        115                 120                 125                       

gga aat aga gaa caa caa ttt ttt ctg tgg ttt gac cct aca gct gac        432
Gly Asn Arg Glu Gln Gln Phe Phe Leu Trp Phe Asp Pro Thr Ala Asp           
    130                 135                 140                           

ttc cat gat tat aca ata att tgg aac cct caa caa ata ttg ttt ctt        480
Phe His Asp Tyr Thr Ile Ile Trp Asn Pro Gln Gln Ile Leu Phe Leu           
145                 150                 155                 160           

gtt gat gga agg gct gtt aga tct ttt ccg aat aat gag gct ata ggt        528
Val Asp Gly Arg Ala Val Arg Ser Phe Pro Asn Asn Glu Ala Ile Gly           
                165                 170                 175               

gtc cct tac tta aaa agt caa tgg atg aat gta cat tta agt ctt tgg        576
Val Pro Tyr Leu Lys Ser Gln Trp Met Asn Val His Leu Ser Leu Trp           
            180                 185                 190                   

aat ggc gag act tgg gcc aca cta gga ggg ttg aga agg ata gat tgg        624
Asn Gly Glu Thr Trp Ala Thr Leu Gly Gly Leu Arg Arg Ile Asp Trp           
        195                 200                 205                       

aat tca gcc cct ttt gta gct tcc tat tct act ttt gta gga gac tca        672
Asn Ser Ala Pro Phe Val Ala Ser Tyr Ser Thr Phe Val Gly Asp Ser           
    210                 215                 220                           

tgc ttc gat agc gca gat tcc ccg tgc atg gcc tca aaa tgg tgg aac        720
Cys Phe Asp Ser Ala Asp Ser Pro Cys Met Ala Ser Lys Trp Trp Asn           
225                 230                 235                 240           

caa gct gca tat caa tct tta agc aca agt gat gcc agc agt att caa        768
Gln Ala Ala Tyr Gln Ser Leu Ser Thr Ser Asp Ala Ser Ser Ile Gln           
                245                 250                 255               

tgg gtt agg gca aat tat ctc aaa tat gac tat tgt tat gat aca aaa        816
Trp Val Arg Ala Asn Tyr Leu Lys Tyr Asp Tyr Cys Tyr Asp Thr Lys           
            260                 265                 270                   

ctc tat ccg aac ggc ttt ccc agc gaa tgc tca aac cgt ggt ttc tag        864
Leu Tyr Pro Asn Gly Phe Pro Ser Glu Cys Ser Asn Arg Gly Phe               
        275                 280                 285                       


<210>  8
<211>  287
<212>  PRT
<213>  Equisetum diffusum

<400>  8

Met Lys Lys Lys Thr Ala Ser Met Leu Gly Leu Ala Phe Gly Met Leu 
1               5                   10                  15      


Leu Ile Met Leu Ala Ser Pro Lys Leu Ala Ile Ala Gly Phe Tyr Glu 
            20                  25                  30          


Asp Phe Asp Val Asp Pro Pro Pro Asp His Val Ile Ile Gln Ser Asp 
        35                  40                  45              


Ser Leu Leu Glu Leu Thr Met Asp Lys Asn Ser Gly Ser Thr Val Val 
    50                  55                  60                  


Ser Thr Arg Lys Tyr Leu Phe Gly Tyr Phe Asn Met Lys Met Lys Leu 
65                  70                  75                  80  


Ile Ser Gly Asn Ser Ala Gly Thr Val Thr Thr Phe Tyr Ile Phe Ser 
                85                  90                  95      


Glu Glu Ala Asn His Asp Glu Ile Asp Phe Glu Phe Leu Gly Asn Tyr 
            100                 105                 110         


Ser Gly Asp Pro Tyr Leu Leu His Thr Asn Ile Phe Ala Ser Gly Val 
        115                 120                 125             


Gly Asn Arg Glu Gln Gln Phe Phe Leu Trp Phe Asp Pro Thr Ala Asp 
    130                 135                 140                 


Phe His Asp Tyr Thr Ile Ile Trp Asn Pro Gln Gln Ile Leu Phe Leu 
145                 150                 155                 160 


Val Asp Gly Arg Ala Val Arg Ser Phe Pro Asn Asn Glu Ala Ile Gly 
                165                 170                 175     


Val Pro Tyr Leu Lys Ser Gln Trp Met Asn Val His Leu Ser Leu Trp 
            180                 185                 190         


Asn Gly Glu Thr Trp Ala Thr Leu Gly Gly Leu Arg Arg Ile Asp Trp 
        195                 200                 205             


Asn Ser Ala Pro Phe Val Ala Ser Tyr Ser Thr Phe Val Gly Asp Ser 
    210                 215                 220                 


Cys Phe Asp Ser Ala Asp Ser Pro Cys Met Ala Ser Lys Trp Trp Asn 
225                 230                 235                 240 


Gln Ala Ala Tyr Gln Ser Leu Ser Thr Ser Asp Ala Ser Ser Ile Gln 
                245                 250                 255     


Trp Val Arg Ala Asn Tyr Leu Lys Tyr Asp Tyr Cys Tyr Asp Thr Lys 
            260                 265                 270         


Leu Tyr Pro Asn Gly Phe Pro Ser Glu Cys Ser Asn Arg Gly Phe 
        275                 280                 285         


