                         SEQUENCE LISTING

<110>  QIAGEN GmbH
 
<120>  DNA-adapter-molecules for the preparation of DNA-libraries and 
       method for producing them and use

<130>  00139P0010EPWO


<150>  EP 13 186 776.4
<151>  2013-09-30

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  57
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor reverse strand

<400>  1
tgtgacttca atttactatg tagcaaagga tactccgacg cggccgcagc atcacga          57


<210>  2
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor reverse strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is 1-hydroxypropyl-3-phosphatidyl


<400>  2
ntgtgacttc aatttactat gtagcaaagg atactccgac gcggccgcag catcacga         58


<210>  3
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor reverse strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is 1,2-dideoxyribosyl-3-phosphate



<400>  3
ntgtgacttc aatttactat gtagcaaagg atactccgac gcggccgcag catcacga         58


<210>  4
<211>  57
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand

<400>  4
tcgtgatgct gcggccgcgt cggagtatcc tttgctacat agtaaattga agtcaca          57


<210>  5
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is 1-hydroxypropyl-3-phosphatidyl 


<220>
<221>  modified_base
<222>  (59)..(59)
<223>  n is 6-aminohexyl-1-phosphate 


<400>  5
ntcgtgatgc tgcggccgcg tcggagtatc ctttgctaca tagtaaattg aagtcacan        59


<210>  6
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is 1-hydroxypropyl-3-phosphatidyl 

<220>
<221>  modified_base
<222>  (59)..(59)
<223>  n is 1-oxy-3-propanol 


<400>  6
ntcgtgatgc tgcggccgcg tcggagtatc ctttgctaca tagtaaattg aagtcacan        59


<210>  7
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is 5'-O-methyl deoxythymidine monophosphate

<220>
<221>  modified_base
<222>  (59)..(59)
<223>  n is 6-aminohexyl-1-phosphate 

<400>  7
ntcgtgatgc tgcggccgcg tcggagtatc ctttgctaca tagtaaattg aagtcacan        59


<210>  8
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is 5'-O-methyl deoxythymidine monophosphate


<220>
<221>  modified_base
<222>  (59)..(59)
<223>  n is 1-oxy-3-propanol


<400>  8
ntcgtgatgc tgcggccgcg tcggagtatc ctttgctaca tagtaaattg aagtcacan        59


<210>  9
<211>  56
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand


<220>
<221>  modified_base
<222>  (54)..(54)
<223>  n is phosphor thioate modified nucleoside A


<220>
<221>  modified_base
<222>  (55)..(55)
<223>  n is phosphor thioate modified nucleoside C

<400>  9
cgtgatgctg cggccgcgtc ggagtatcct ttgctacata gtaaattgaa gtcnna           56


<210>  10
<211>  52
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor reverse strand

<400>  10
acttcaattt actatgtagc aaaggatact ccgacgcggc cgcagcatca cg               52


<210>  11
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is etherified deoxynucleotide or 4'-Amino-deoxynucleotide or 
       5'-Amino-deoxynucleotide or 5'-C3-Spacer or 5'-D-Spacer


<220>
<221>  modified_base
<222>  (58)..(58)
<223>  n is  3'-Amino-Modifier or 3'-C3-Spacer


<400>  11
ncgtgatgct gcggccgcgt cggagtatcc tttgctacat agtaaattga agtcacan         58


<210>  12
<211>  57
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor reverse strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is etherified deoxynucleotide or 4'-Amino-deoxynucleotide or 
       5'-Amino-deoxynucleotide or 5'-C3-Spacer or 5'-D-Spacer


<400>  12
ntgtgacttc aatttactat gtagcaaagg atactccgac gcggccgcag catcacg          57


<210>  13
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is 5'-O-methyl deoxythymidine monophosphate


<220>
<221>  modified_base
<222>  (58)..(58)
<223>  n is etherified deoxynucleotide or 4'-Amino-deoxynucleotide or 
       5'-Amino-deoxynucleotide or 5'-C3-Spacer or 5'-D-Spacer


<400>  13
ncgtgatgct gcggccgcgt cggagtatcc tttgctacat agtaaattga agtcacan         58


<210>  14
<211>  58
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor reverse strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  n is etherified deoxynucleotide or 4'-Amino-deoxynucleotide or 
       5'-Amino-deoxynucleotide or 5'-C3-Spacer or 5'-D-Spacer


<400>  14
ntgtgacttc aatttactat gtagcaaagg atactccgac gcggccgcag catcacga         58


<210>  15
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor forward strand

<400>  15
gtaaaacgac ggccagt                                                      17


<210>  16
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  Universal DNA adaptor reverse strand


<220>
<221>  n
<222>  (17)..(17)
<223>  n is 2'-Deoxycytosine-5'-O-monophosphorthioate


<220>
<221>  n
<222>  (18)..(18)
<223>  n is 2'-Deoxythymidine-5'-O-monophosphorthioate

<400>  16
actggccgtc gttttannt                                                    19


