                         SEQUENCE LISTING

<110>  Ohio State Innovation Foundation
       Kaur, Balveen
 
<120>  MUTANT CHONDROITINASE ABC I COMPOSITIONS AND METHODS OF THEIR USE

<130>  26227.0065P1

<150>  61/883,436
<151>  2013-09-27

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  3055
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant Chase ABC

<400>  1
catggagaca gacacactcc tgctatgggt actgctgctc tgggttccag gttccactgg       60

tgacaccagc aatcctgcat ttgatcctaa aaatctgatg cagtcagaaa tttaccattt      120

tgcacaaaat aacccattag cagacttctc atcagataaa aactcaatac taacgttatc      180

tgataaacgt agcattatgg gaaaccaatc tcttttatgg aaatggaaag gtggtagtag      240

ctttacttta cataaaaaac tgattgtccc caccgataaa gaagcatcta aagcatgggg      300

acgctcatct acccccgttt tctcattttg gctttacaat gaaaaaccga ttgatggtta      360

tcttactatc gatttcggag aaaaactcat ttcaaccagt gaggctcagg caggctttaa      420

agtaaaatta gatttcactg gctggcgtgc tgtgggagtc tctttaaata acgatcttga      480

aaatcgagag atgaccttaa atgcaaccaa tacctcctct gatggtactc aagacagcat      540

tgggcgttct ttaggtgcta aagtcgatag tattcgtttt aaagcgcctt ctaatgtgag      600

tcagggtgaa atctatatcg accgtattat gttttctgtc gatgatgctc gctaccaatg      660

gtctgattat caagtaaaaa ctcgcttatc agaacctgaa attcaatttc acaacgtaaa      720

gccacaacta cctgtaacac ctgaaaattt agcggccatt gatcttattc gccaacgtct      780

aattaatgaa tttgtcggag gtgaaaaaga gacaaacctc gcattagaag agcagatcag      840

caaattaaaa agtgatttcg atgctcttaa tattcacact ttagcaaatg gtggaacgca      900

aggcagacat ctgatcactg ataaacaaat cattatttat caaccagaga atcttaactc      960

ccaagataaa caactatttg ataattatgt tattttaggt cagtacacga cactaatgtt     1020

tcagattagc cgtgcttatg tgctggaaaa agatcccaca caaaaggcgc aactaaagca     1080

gatgtactta ttaatgacaa agcatttatt agatcaaggc tttgttaaag ggagtgcttt     1140

agtgacaacc catcactggg gatacagttc tcgttggtgg tatatttcca cgttattaat     1200

gtctgatgca ctaaaagaag cgaacctaca aactcaagtt tatgattcat tactgtggta     1260

ttcacgtgag tttaaaagta gttttgatat gaaagtaagt gctgatagct ctgatctaga     1320

ttatttcaat accttatctc gccaacattt agccttatta ttactagagc ctgatgatca     1380

aaagcgtatc aacttagtta atactttcag ccattatatc actggcgcat taacgcaagt     1440

gccaccgggt ggtaaagatg gtttacgccc tgatggtaca gcatggcgac atgaaggcaa     1500

ctatccgggc tactctttcc cagcctttaa aaatgccgct cagctgattt atctgttacg     1560

cgatacacca ttttcagtgg gtgaaagtgg ttggaataac ctgaaaaaag cgatggtttc     1620

agcgtggatc tacagtaatc cagaagttgg attaccgctt gcaggaagac acccttttaa     1680

ctcaccttcg ttaaaatcag tcgctcaagg ctattactgg cttgccatgt ctgcaaaatc     1740

atcgcctgat aaaacacttg catctattta tcttgcgatt agtgataaaa cacaaaatga     1800

atcaactgct atttttggag aaactattac accagcgtct ttacctcaag gtttctatgc     1860

ctttaatggc ggtgcttttg gtattcatcg ttggcaagat aaaatggtga cactgaaagc     1920

ttataacacc aatgtttggt catctgaaat ttataacaaa gataaccgtt atggccgtta     1980

ccaaagtcat ggtgtcgctc aaatagtgag tcagggcgcg cagctgtcac agggctatca     2040

gcaagaaggt tgggattgga atagaatgca aggggcaacc actattcacc ttcctcttaa     2100

agacttagac agtcctaaac ctcatacctt aatgcaacgt ggagagcgtg gatttagcgg     2160

aacatcatcc cttgaaggtc aatatggcat gatggcattc gatcttattt atcccgccaa     2220

tcttgagcgt tttgatccta atttcactgc gaaaaagagt gtattagccg ctgataatca     2280

cttaattttt attggtagca atataaatag tagtgataaa aataaaaatg ttgaaacgac     2340

cttattccaa catgccatta ctccaacatt aaataccctt tggattaatg gacaaaagat     2400

agaaaacatg ccttatcaaa caacacttca acaaggtgat tggttaattg atagcaatgg     2460

caatggttac ttaattactc aagcagaaaa agtaaatgta agtcgccaac atcaggtttc     2520

agcggaaaat aaaaatcgcc aaccgacaga aggaaacttt agctcggcat ggatcgatca     2580

cagcactcgc cccaaagatg ccagttatga gtatatggtc tttttagatg cgacacctga     2640

aaaaatggga gagatggcac aaaaattccg tgaaaataat gggttatatc aggttcttcg     2700

taaggataaa gacgttcata ttattctcga taaactcagc aatgtaacgg gatatgcctt     2760

ttatcagcca gcatcaattg aagacaaatg gatcaaaaag gttaataaac ctgcaattgt     2820

gatgactcat cgacaaaaag acactcttat tgtcagtgca gttacacctg atttaaatat     2880

gactcgccaa aaagcagcaa ctcctgtcac catcaatgtc acgattaatg gcaaatggca     2940

atctgctgat aaaaatagtg aagtgaaata tcaggtttct ggtgataaca ctgaactgac     3000

gtttacgagt tactttggta ttccacaaga aatcaaactc tcgccactcc cttga          3055


<210>  2
<211>  2991
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant Chase ABC without a signal sequence

<400>  2
accagcaatc ctgcatttga tcctaaaaat ctgatgcagt cagaaattta ccattttgca       60

caaaataacc cattagcaga cttctcatca gataaaaact caatactaac gttatctgat      120

aaacgtagca ttatgggaaa ccaatctctt ttatggaaat ggaaaggtgg tagtagcttt      180

actttacata aaaaactgat tgtccccacc gataaagaag catctaaagc atggggacgc      240

tcatctaccc ccgttttctc attttggctt tacaatgaaa aaccgattga tggttatctt      300

actatcgatt tcggagaaaa actcatttca accagtgagg ctcaggcagg ctttaaagta      360

aaattagatt tcactggctg gcgtgctgtg ggagtctctt taaataacga tcttgaaaat      420

cgagagatga ccttaaatgc aaccaatacc tcctctgatg gtactcaaga cagcattggg      480

cgttctttag gtgctaaagt cgatagtatt cgttttaaag cgccttctaa tgtgagtcag      540

ggtgaaatct atatcgaccg tattatgttt tctgtcgatg atgctcgcta ccaatggtct      600

gattatcaag taaaaactcg cttatcagaa cctgaaattc aatttcacaa cgtaaagcca      660

caactacctg taacacctga aaatttagcg gccattgatc ttattcgcca acgtctaatt      720

aatgaatttg tcggaggtga aaaagagaca aacctcgcat tagaagagca gatcagcaaa      780

ttaaaaagtg atttcgatgc tcttaatatt cacactttag caaatggtgg aacgcaaggc      840

agacatctga tcactgataa acaaatcatt atttatcaac cagagaatct taactcccaa      900

gataaacaac tatttgataa ttatgttatt ttaggtcagt acacgacact aatgtttcag      960

attagccgtg cttatgtgct ggaaaaagat cccacacaaa aggcgcaact aaagcagatg     1020

tacttattaa tgacaaagca tttattagat caaggctttg ttaaagggag tgctttagtg     1080

acaacccatc actggggata cagttctcgt tggtggtata tttccacgtt attaatgtct     1140

gatgcactaa aagaagcgaa cctacaaact caagtttatg attcattact gtggtattca     1200

cgtgagttta aaagtagttt tgatatgaaa gtaagtgctg atagctctga tctagattat     1260

ttcaatacct tatctcgcca acatttagcc ttattattac tagagcctga tgatcaaaag     1320

cgtatcaact tagttaatac tttcagccat tatatcactg gcgcattaac gcaagtgcca     1380

ccgggtggta aagatggttt acgccctgat ggtacagcat ggcgacatga aggcaactat     1440

ccgggctact ctttcccagc ctttaaaaat gccgctcagc tgatttatct gttacgcgat     1500

acaccatttt cagtgggtga aagtggttgg aataacctga aaaaagcgat ggtttcagcg     1560

tggatctaca gtaatccaga agttggatta ccgcttgcag gaagacaccc ttttaactca     1620

ccttcgttaa aatcagtcgc tcaaggctat tactggcttg ccatgtctgc aaaatcatcg     1680

cctgataaaa cacttgcatc tatttatctt gcgattagtg ataaaacaca aaatgaatca     1740

actgctattt ttggagaaac tattacacca gcgtctttac ctcaaggttt ctatgccttt     1800

aatggcggtg cttttggtat tcatcgttgg caagataaaa tggtgacact gaaagcttat     1860

aacaccaatg tttggtcatc tgaaatttat aacaaagata accgttatgg ccgttaccaa     1920

agtcatggtg tcgctcaaat agtgagtcag ggcgcgcagc tgtcacaggg ctatcagcaa     1980

gaaggttggg attggaatag aatgcaaggg gcaaccacta ttcaccttcc tcttaaagac     2040

ttagacagtc ctaaacctca taccttaatg caacgtggag agcgtggatt tagcggaaca     2100

tcatcccttg aaggtcaata tggcatgatg gcattcgatc ttatttatcc cgccaatctt     2160

gagcgttttg atcctaattt cactgcgaaa aagagtgtat tagccgctga taatcactta     2220

atttttattg gtagcaatat aaatagtagt gataaaaata aaaatgttga aacgacctta     2280

ttccaacatg ccattactcc aacattaaat accctttgga ttaatggaca aaagatagaa     2340

aacatgcctt atcaaacaac acttcaacaa ggtgattggt taattgatag caatggcaat     2400

ggttacttaa ttactcaagc agaaaaagta aatgtaagtc gccaacatca ggtttcagcg     2460

gaaaataaaa atcgccaacc gacagaagga aactttagct cggcatggat cgatcacagc     2520

actcgcccca aagatgccag ttatgagtat atggtctttt tagatgcgac acctgaaaaa     2580

atgggagaga tggcacaaaa attccgtgaa aataatgggt tatatcaggt tcttcgtaag     2640

gataaagacg ttcatattat tctcgataaa ctcagcaatg taacgggata tgccttttat     2700

cagccagcat caattgaaga caaatggatc aaaaaggtta ataaacctgc aattgtgatg     2760

actcatcgac aaaaagacac tcttattgtc agtgcagtta cacctgattt aaatatgact     2820

cgccaaaaag cagcaactcc tgtcaccatc aatgtcacga ttaatggcaa atggcaatct     2880

gctgataaaa atagtgaagt gaaatatcag gtttctggtg ataacactga actgacgttt     2940

acgagttact ttggtattcc acaagaaatc aaactctcgc cactcccttg a              2991


<210>  3
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; IgKappa sequence of mutant Chase ABC

<400>  3
catggagaca gacacactcc tgctatgggt actgctgctc tgggttccag gttccactgg       60

t                                                                       61


<210>  4
<211>  2988
<212>  DNA
<213>  Homo sapiens

<400>  4
accagcaacc cggcgtttga tccgaaaaac ctgatgcaga gcgaaattta tcattttgcg       60

cagaacaacc cgctggcgga ttttagcagc gataaaaaca gcattctgac cctgagcgat      120

aaacgcagca ttatgggcaa ccagagcctg ctgtggaaat ggaaaggcgg cagcagcttt      180

accctgcata aaaaactgat tgtgccgacc gataaagaag cgagcaaagc gtggggccgc      240

agcagcaccc cggtgtttag cttttggctg tataacgaaa aaccgattga tggctatctg      300

accattgatt ttggcgaaaa actgattagc accagcgaag cgcaggcggg ctttaaagtg      360

aaactggatt ttaccggctg gcgcgcggtg ggcgtgagcc tgaacaacga tctggaaaac      420

cgcgaaatga ccctgaacgc gaccaacacc agcagcgatg gcacccagga tagcattggc      480

cgcagcctgg gcgcgaaagt ggatagcatt cgctttaaag cgccgagcaa cgtgagccag      540

ggcgaaattt atattgatcg cattatgttt agcgtggatg atgcgcgcta tcagtggagc      600

gattatcagg tgaaaacccg cctgagcgaa ccggaaattc agtttcataa cgtgaaaccg      660

cagctgccgg tgaccccgga aaacctggcg gcgattgatc tgattcgcca gcgcctgatt      720

aacgaatttg tgggcggcga aaaagaaacc aacctggcgc tggaagaaaa cattagcaaa      780

ctgaaaagcg attttgatgc gctgaacatt cataccctgg cgaacggcgg cacccagggc      840

cgccatctga ttaccgataa acagattatt atttatcagc cggaaaacct gaacagccag      900

gataaacagc tgtttgataa ctatgtgatt ctgggcaact ataccaccct gatgtttaac      960

attagccgcg cgtatgtgct ggaaaaagat ccgacccaga aagcgcagct gaaacagatg     1020

tatctgctga tgaccaaaca tctgctggat cagggctttg tgaaaggcag cgcgctggtg     1080

accacccatc attggggcta tagcagccgc tggtggtata ttagcaccct gctgatgagc     1140

gatgcgctga aagaagcgaa cctgcagacc caggtgtatg atagcctgct gtggtatagc     1200

cgcgaattta aaagcagctt tgatatgaaa gtgagcgcgg atagcagcga tctggattat     1260

tttaacaccc tgagccgcca gcatctggcg ctgctgctgc tggaaccgga tgatcagaaa     1320

cgcattaacc tggtgaacac ctttagccat tatattaccg gcgcgctgac ccaggtgccg     1380

ccgggcggca aagatggcct gcgcccggat ggcaccgcgt ggcgccatga aggcaactat     1440

ccgggctata gctttccggc gtttaaaaac gcgagccagc tgatttatct gctgcgcgat     1500

accccgttta gcgtgggcga aagcggctgg aacaacctga aaaaagcgat ggtgagcgcg     1560

tggatttata gcaacccgga agtgggcctg ccgctggcgg gccgccatcc gtttaacagc     1620

ccgagcctga aaagcgtggc gcagggctat tattggctgg cgatgagcgc gaaaagcagc     1680

ccggataaaa ccctggcgag catttatctg gcgattagcg ataaaaccca gaacgaaagc     1740

accgcgattt ttggcgaaac cattaccccg gcgagcctgc cgcagggctt ttatgcgttt     1800

aacggcggcg cgtttggcat tcatcgctgg caggataaaa tggtgaccct gaaagcgtat     1860

aacaccaacg tgtggagcag cgaaatttat aacaaagata accgctatgg ccgctatcag     1920

agccatggcg tggcgcagat tgtgagcaac ggcagccagc tgagccaggg ctatcagcag     1980

gaaggctggg attggaaccg catgcagggc gcgaccacca ttcatctgcc gctgaaagat     2040

ctggatagcc cgaaaccgca taccctgatg cagcgcggcg aacgcggctt tagcggcacc     2100

agcagcctgg aaggccagta tggcatgatg gcgtttgatc tgatttatcc ggcgaacctg     2160

gaacgctttg atccgaactt taccgcgaaa aaaagcgtgc tggcggcgga taaccatctg     2220

atttttattg gcagcaacat taacagcagc gataaaaaca aaaacgtgga aaccaccctg     2280

tttcagcatg cgattacccc gaccctgaac accctgtgga ttaacggcca gaaaattgaa     2340

aacatgccgt atcagaccac cctgcagcag ggcgattggc tgattgatag caacggcaac     2400

ggctatctga ttacccaggc ggaaaaagtg aacgtgagcc gccagcatca ggtgagcgcg     2460

gaaaacaaaa accgccagcc gaccgaaggc aactttagca gcgcgtggat tgatcatagc     2520

acccgcccga aagatgcgag ctatgaatat atggtgtttc tggatgcgac cccggaaaaa     2580

atgggcgaaa tggcgcagaa atttcgcgaa aacaacggcc tgtatcaggt gctgcgcaaa     2640

gataaagatg tgcatattat tctggataaa ctgagcaacg tgaccggcta tgcgttttat     2700

cagccggcga gcattgaaga taaatggatt aaaaaagtga acaaaccggc gattgtgatg     2760

acccatcgcc agaaagatac cctgattgtg agcgcggtga ccccggatct gaacatgacc     2820

cgccagaaag cggcgacccc ggtgaccatt aacgtgacca ttaacggcaa atggcagagc     2880

gcggataaaa acagcgaagt gaaatatcag gtgagcggcg ataacaccga actgaccttt     2940

accagctatt ttggcattcc gcaggaaatt aaactgagcc cgctgccg                  2988


<210>  5
<211>  1017
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant Chase ABC plus a signal sequence

<400>  5

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Thr Ser Asn Pro Ala Phe Asp Pro Lys Asn Leu 
            20                  25                  30          


Met Gln Ser Glu Ile Tyr His Phe Ala Gln Asn Asn Pro Leu Ala Asp 
        35                  40                  45              


Phe Ser Ser Asp Lys Asn Ser Ile Leu Thr Leu Ser Asp Lys Arg Ser 
    50                  55                  60                  


Ile Met Gly Asn Gln Ser Leu Leu Trp Lys Trp Lys Gly Gly Ser Ser 
65                  70                  75                  80  


Phe Thr Leu His Lys Lys Leu Ile Val Pro Thr Asp Lys Glu Ala Ser 
                85                  90                  95      


Lys Ala Trp Gly Arg Ser Ser Thr Pro Val Phe Ser Phe Trp Leu Tyr 
            100                 105                 110         


Asn Glu Lys Pro Ile Asp Gly Tyr Leu Thr Ile Asp Phe Gly Glu Lys 
        115                 120                 125             


Leu Ile Ser Thr Ser Glu Ala Gln Ala Gly Phe Lys Val Lys Leu Asp 
    130                 135                 140                 


Phe Thr Gly Trp Arg Ala Val Gly Val Ser Leu Asn Asn Asp Leu Glu 
145                 150                 155                 160 


Asn Arg Glu Met Thr Leu Asn Ala Thr Asn Thr Ser Ser Asp Gly Thr 
                165                 170                 175     


Gln Asp Ser Ile Gly Arg Ser Leu Gly Ala Lys Val Asp Ser Ile Arg 
            180                 185                 190         


Phe Lys Ala Pro Ser Asn Val Ser Gln Gly Glu Ile Tyr Ile Asp Arg 
        195                 200                 205             


Ile Met Phe Ser Val Asp Asp Ala Arg Tyr Gln Trp Ser Asp Tyr Gln 
    210                 215                 220                 


Val Lys Thr Arg Leu Ser Glu Pro Glu Ile Gln Phe His Asn Val Lys 
225                 230                 235                 240 


Pro Gln Leu Pro Val Thr Pro Glu Asn Leu Ala Ala Ile Asp Leu Ile 
                245                 250                 255     


Arg Gln Arg Leu Ile Asn Glu Phe Val Gly Gly Glu Lys Glu Thr Asn 
            260                 265                 270         


Leu Ala Leu Glu Glu Gln Ile Ser Lys Leu Lys Ser Asp Phe Asp Ala 
        275                 280                 285             


Leu Asn Ile His Thr Leu Ala Asn Gly Gly Thr Gln Gly Arg His Leu 
    290                 295                 300                 


Ile Thr Asp Lys Gln Ile Ile Ile Tyr Gln Pro Glu Asn Leu Asn Ser 
305                 310                 315                 320 


Gln Asp Lys Gln Leu Phe Asp Asn Tyr Val Ile Leu Gly Gln Tyr Thr 
                325                 330                 335     


Thr Leu Met Phe Gln Ile Ser Arg Ala Tyr Val Leu Glu Lys Asp Pro 
            340                 345                 350         


Thr Gln Lys Ala Gln Leu Lys Gln Met Tyr Leu Leu Met Thr Lys His 
        355                 360                 365             


Leu Leu Asp Gln Gly Phe Val Lys Gly Ser Ala Leu Val Thr Thr His 
    370                 375                 380                 


His Trp Gly Tyr Ser Ser Arg Trp Trp Tyr Ile Ser Thr Leu Leu Met 
385                 390                 395                 400 


Ser Asp Ala Leu Lys Glu Ala Asn Leu Gln Thr Gln Val Tyr Asp Ser 
                405                 410                 415     


Leu Leu Trp Tyr Ser Arg Glu Phe Lys Ser Ser Phe Asp Met Lys Val 
            420                 425                 430         


Ser Ala Asp Ser Ser Asp Leu Asp Tyr Phe Asn Thr Leu Ser Arg Gln 
        435                 440                 445             


His Leu Ala Leu Leu Leu Leu Glu Pro Asp Asp Gln Lys Arg Ile Asn 
    450                 455                 460                 


Leu Val Asn Thr Phe Ser His Tyr Ile Thr Gly Ala Leu Thr Gln Val 
465                 470                 475                 480 


Pro Pro Gly Gly Lys Asp Gly Leu Arg Pro Asp Gly Thr Ala Trp Arg 
                485                 490                 495     


His Glu Gly Asn Tyr Pro Gly Tyr Ser Phe Pro Ala Phe Lys Asn Ala 
            500                 505                 510         


Ala Gln Leu Ile Tyr Leu Leu Arg Asp Thr Pro Phe Ser Val Gly Glu 
        515                 520                 525             


Ser Gly Trp Asn Asn Leu Lys Lys Ala Met Val Ser Ala Trp Ile Tyr 
    530                 535                 540                 


Ser Asn Pro Glu Val Gly Leu Pro Leu Ala Gly Arg His Pro Phe Asn 
545                 550                 555                 560 


Ser Pro Ser Leu Lys Ser Val Ala Gln Gly Tyr Tyr Trp Leu Ala Met 
                565                 570                 575     


Ser Ala Lys Ser Ser Pro Asp Lys Thr Leu Ala Ser Ile Tyr Leu Ala 
            580                 585                 590         


Ile Ser Asp Lys Thr Gln Asn Glu Ser Thr Ala Ile Phe Gly Glu Thr 
        595                 600                 605             


Ile Thr Pro Ala Ser Leu Pro Gln Gly Phe Tyr Ala Phe Asn Gly Gly 
    610                 615                 620                 


Ala Phe Gly Ile His Arg Trp Gln Asp Lys Met Val Thr Leu Lys Ala 
625                 630                 635                 640 


Tyr Asn Thr Asn Val Trp Ser Ser Glu Ile Tyr Asn Lys Asp Asn Arg 
                645                 650                 655     


Tyr Gly Arg Tyr Gln Ser His Gly Val Ala Gln Ile Val Ser Gln Gly 
            660                 665                 670         


Ala Gln Leu Ser Gln Gly Tyr Gln Gln Glu Gly Trp Asp Trp Asn Arg 
        675                 680                 685             


Met Gln Gly Ala Thr Thr Ile His Leu Pro Leu Lys Asp Leu Asp Ser 
    690                 695                 700                 


Pro Lys Pro His Thr Leu Met Gln Arg Gly Glu Arg Gly Phe Ser Gly 
705                 710                 715                 720 


Thr Ser Ser Leu Glu Gly Gln Tyr Gly Met Met Ala Phe Asp Leu Ile 
                725                 730                 735     


Tyr Pro Ala Asn Leu Glu Arg Phe Asp Pro Asn Phe Thr Ala Lys Lys 
            740                 745                 750         


Ser Val Leu Ala Ala Asp Asn His Leu Ile Phe Ile Gly Ser Asn Ile 
        755                 760                 765             


Asn Ser Ser Asp Lys Asn Lys Asn Val Glu Thr Thr Leu Phe Gln His 
    770                 775                 780                 


Ala Ile Thr Pro Thr Leu Asn Thr Leu Trp Ile Asn Gly Gln Lys Ile 
785                 790                 795                 800 


Glu Asn Met Pro Tyr Gln Thr Thr Leu Gln Gln Gly Asp Trp Leu Ile 
                805                 810                 815     


Asp Ser Asn Gly Asn Gly Tyr Leu Ile Thr Gln Ala Glu Lys Val Asn 
            820                 825                 830         


Val Ser Arg Gln His Gln Val Ser Ala Glu Asn Lys Asn Arg Gln Pro 
        835                 840                 845             


Thr Glu Gly Asn Phe Ser Ser Ala Trp Ile Asp His Ser Thr Arg Pro 
    850                 855                 860                 


Lys Asp Ala Ser Tyr Glu Tyr Met Val Phe Leu Asp Ala Thr Pro Glu 
865                 870                 875                 880 


Lys Met Gly Glu Met Ala Gln Lys Phe Arg Glu Asn Asn Gly Leu Tyr 
                885                 890                 895     


Gln Val Leu Arg Lys Asp Lys Asp Val His Ile Ile Leu Asp Lys Leu 
            900                 905                 910         


Ser Asn Val Thr Gly Tyr Ala Phe Tyr Gln Pro Ala Ser Ile Glu Asp 
        915                 920                 925             


Lys Trp Ile Lys Lys Val Asn Lys Pro Ala Ile Val Met Thr His Arg 
    930                 935                 940                 


Gln Lys Asp Thr Leu Ile Val Ser Ala Val Thr Pro Asp Leu Asn Met 
945                 950                 955                 960 


Thr Arg Gln Lys Ala Ala Thr Pro Val Thr Ile Asn Val Thr Ile Asn 
                965                 970                 975     


Gly Lys Trp Gln Ser Ala Asp Lys Asn Ser Glu Val Lys Tyr Gln Val 
            980                 985                 990         


Ser Gly Asp Asn Thr Glu Leu Thr  Phe Thr Ser Tyr Phe  Gly Ile Pro 
        995                 1000                 1005             


Gln Glu  Ile Lys Leu Ser Pro  Leu Pro 
    1010                 1015         


<210>  6
<211>  996
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant Chase ABC

<400>  6

Thr Ser Asn Pro Ala Phe Asp Pro Lys Asn Leu Met Gln Ser Glu Ile 
1               5                   10                  15      


Tyr His Phe Ala Gln Asn Asn Pro Leu Ala Asp Phe Ser Ser Asp Lys 
            20                  25                  30          


Asn Ser Ile Leu Thr Leu Ser Asp Lys Arg Ser Ile Met Gly Asn Gln 
        35                  40                  45              


Ser Leu Leu Trp Lys Trp Lys Gly Gly Ser Ser Phe Thr Leu His Lys 
    50                  55                  60                  


Lys Leu Ile Val Pro Thr Asp Lys Glu Ala Ser Lys Ala Trp Gly Arg 
65                  70                  75                  80  


Ser Ser Thr Pro Val Phe Ser Phe Trp Leu Tyr Asn Glu Lys Pro Ile 
                85                  90                  95      


Asp Gly Tyr Leu Thr Ile Asp Phe Gly Glu Lys Leu Ile Ser Thr Ser 
            100                 105                 110         


Glu Ala Gln Ala Gly Phe Lys Val Lys Leu Asp Phe Thr Gly Trp Arg 
        115                 120                 125             


Ala Val Gly Val Ser Leu Asn Asn Asp Leu Glu Asn Arg Glu Met Thr 
    130                 135                 140                 


Leu Asn Ala Thr Asn Thr Ser Ser Asp Gly Thr Gln Asp Ser Ile Gly 
145                 150                 155                 160 


Arg Ser Leu Gly Ala Lys Val Asp Ser Ile Arg Phe Lys Ala Pro Ser 
                165                 170                 175     


Asn Val Ser Gln Gly Glu Ile Tyr Ile Asp Arg Ile Met Phe Ser Val 
            180                 185                 190         


Asp Asp Ala Arg Tyr Gln Trp Ser Asp Tyr Gln Val Lys Thr Arg Leu 
        195                 200                 205             


Ser Glu Pro Glu Ile Gln Phe His Asn Val Lys Pro Gln Leu Pro Val 
    210                 215                 220                 


Thr Pro Glu Asn Leu Ala Ala Ile Asp Leu Ile Arg Gln Arg Leu Ile 
225                 230                 235                 240 


Asn Glu Phe Val Gly Gly Glu Lys Glu Thr Asn Leu Ala Leu Glu Glu 
                245                 250                 255     


Gln Ile Ser Lys Leu Lys Ser Asp Phe Asp Ala Leu Asn Ile His Thr 
            260                 265                 270         


Leu Ala Asn Gly Gly Thr Gln Gly Arg His Leu Ile Thr Asp Lys Gln 
        275                 280                 285             


Ile Ile Ile Tyr Gln Pro Glu Asn Leu Asn Ser Gln Asp Lys Gln Leu 
    290                 295                 300                 


Phe Asp Asn Tyr Val Ile Leu Gly Gln Tyr Thr Thr Leu Met Phe Gln 
305                 310                 315                 320 


Ile Ser Arg Ala Tyr Val Leu Glu Lys Asp Pro Thr Gln Lys Ala Gln 
                325                 330                 335     


Leu Lys Gln Met Tyr Leu Leu Met Thr Lys His Leu Leu Asp Gln Gly 
            340                 345                 350         


Phe Val Lys Gly Ser Ala Leu Val Thr Thr His His Trp Gly Tyr Ser 
        355                 360                 365             


Ser Arg Trp Trp Tyr Ile Ser Thr Leu Leu Met Ser Asp Ala Leu Lys 
    370                 375                 380                 


Glu Ala Asn Leu Gln Thr Gln Val Tyr Asp Ser Leu Leu Trp Tyr Ser 
385                 390                 395                 400 


Arg Glu Phe Lys Ser Ser Phe Asp Met Lys Val Ser Ala Asp Ser Ser 
                405                 410                 415     


Asp Leu Asp Tyr Phe Asn Thr Leu Ser Arg Gln His Leu Ala Leu Leu 
            420                 425                 430         


Leu Leu Glu Pro Asp Asp Gln Lys Arg Ile Asn Leu Val Asn Thr Phe 
        435                 440                 445             


Ser His Tyr Ile Thr Gly Ala Leu Thr Gln Val Pro Pro Gly Gly Lys 
    450                 455                 460                 


Asp Gly Leu Arg Pro Asp Gly Thr Ala Trp Arg His Glu Gly Asn Tyr 
465                 470                 475                 480 


Pro Gly Tyr Ser Phe Pro Ala Phe Lys Asn Ala Ala Gln Leu Ile Tyr 
                485                 490                 495     


Leu Leu Arg Asp Thr Pro Phe Ser Val Gly Glu Ser Gly Trp Asn Asn 
            500                 505                 510         


Leu Lys Lys Ala Met Val Ser Ala Trp Ile Tyr Ser Asn Pro Glu Val 
        515                 520                 525             


Gly Leu Pro Leu Ala Gly Arg His Pro Phe Asn Ser Pro Ser Leu Lys 
    530                 535                 540                 


Ser Val Ala Gln Gly Tyr Tyr Trp Leu Ala Met Ser Ala Lys Ser Ser 
545                 550                 555                 560 


Pro Asp Lys Thr Leu Ala Ser Ile Tyr Leu Ala Ile Ser Asp Lys Thr 
                565                 570                 575     


Gln Asn Glu Ser Thr Ala Ile Phe Gly Glu Thr Ile Thr Pro Ala Ser 
            580                 585                 590         


Leu Pro Gln Gly Phe Tyr Ala Phe Asn Gly Gly Ala Phe Gly Ile His 
        595                 600                 605             


Arg Trp Gln Asp Lys Met Val Thr Leu Lys Ala Tyr Asn Thr Asn Val 
    610                 615                 620                 


Trp Ser Ser Glu Ile Tyr Asn Lys Asp Asn Arg Tyr Gly Arg Tyr Gln 
625                 630                 635                 640 


Ser His Gly Val Ala Gln Ile Val Ser Gln Gly Ala Gln Leu Ser Gln 
                645                 650                 655     


Gly Tyr Gln Gln Glu Gly Trp Asp Trp Asn Arg Met Gln Gly Ala Thr 
            660                 665                 670         


Thr Ile His Leu Pro Leu Lys Asp Leu Asp Ser Pro Lys Pro His Thr 
        675                 680                 685             


Leu Met Gln Arg Gly Glu Arg Gly Phe Ser Gly Thr Ser Ser Leu Glu 
    690                 695                 700                 


Gly Gln Tyr Gly Met Met Ala Phe Asp Leu Ile Tyr Pro Ala Asn Leu 
705                 710                 715                 720 


Glu Arg Phe Asp Pro Asn Phe Thr Ala Lys Lys Ser Val Leu Ala Ala 
                725                 730                 735     


Asp Asn His Leu Ile Phe Ile Gly Ser Asn Ile Asn Ser Ser Asp Lys 
            740                 745                 750         


Asn Lys Asn Val Glu Thr Thr Leu Phe Gln His Ala Ile Thr Pro Thr 
        755                 760                 765             


Leu Asn Thr Leu Trp Ile Asn Gly Gln Lys Ile Glu Asn Met Pro Tyr 
    770                 775                 780                 


Gln Thr Thr Leu Gln Gln Gly Asp Trp Leu Ile Asp Ser Asn Gly Asn 
785                 790                 795                 800 


Gly Tyr Leu Ile Thr Gln Ala Glu Lys Val Asn Val Ser Arg Gln His 
                805                 810                 815     


Gln Val Ser Ala Glu Asn Lys Asn Arg Gln Pro Thr Glu Gly Asn Phe 
            820                 825                 830         


Ser Ser Ala Trp Ile Asp His Ser Thr Arg Pro Lys Asp Ala Ser Tyr 
        835                 840                 845             


Glu Tyr Met Val Phe Leu Asp Ala Thr Pro Glu Lys Met Gly Glu Met 
    850                 855                 860                 


Ala Gln Lys Phe Arg Glu Asn Asn Gly Leu Tyr Gln Val Leu Arg Lys 
865                 870                 875                 880 


Asp Lys Asp Val His Ile Ile Leu Asp Lys Leu Ser Asn Val Thr Gly 
                885                 890                 895     


Tyr Ala Phe Tyr Gln Pro Ala Ser Ile Glu Asp Lys Trp Ile Lys Lys 
            900                 905                 910         


Val Asn Lys Pro Ala Ile Val Met Thr His Arg Gln Lys Asp Thr Leu 
        915                 920                 925             


Ile Val Ser Ala Val Thr Pro Asp Leu Asn Met Thr Arg Gln Lys Ala 
    930                 935                 940                 


Ala Thr Pro Val Thr Ile Asn Val Thr Ile Asn Gly Lys Trp Gln Ser 
945                 950                 955                 960 


Ala Asp Lys Asn Ser Glu Val Lys Tyr Gln Val Ser Gly Asp Asn Thr 
                965                 970                 975     


Glu Leu Thr Phe Thr Ser Tyr Phe Gly Ile Pro Gln Glu Ile Lys Leu 
            980                 985                 990         


Ser Pro Leu Pro 
        995     


<210>  7
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; signal sequence from Igkappa

<400>  7

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  8
<211>  996
<212>  PRT
<213>  Proteus vulgaris

<400>  8

Thr Ser Asn Pro Ala Phe Asp Pro Lys Asn Leu Met Gln Ser Glu Ile 
1               5                   10                  15      


Tyr His Phe Ala Gln Asn Asn Pro Leu Ala Asp Phe Ser Ser Asp Lys 
            20                  25                  30          


Asn Ser Ile Leu Thr Leu Ser Asp Lys Arg Ser Ile Met Gly Asn Gln 
        35                  40                  45              


Ser Leu Leu Trp Lys Trp Lys Gly Gly Ser Ser Phe Thr Leu His Lys 
    50                  55                  60                  


Lys Leu Ile Val Pro Thr Asp Lys Glu Ala Ser Lys Ala Trp Gly Arg 
65                  70                  75                  80  


Ser Ser Thr Pro Val Phe Ser Phe Trp Leu Tyr Asn Glu Lys Pro Ile 
                85                  90                  95      


Asp Gly Tyr Leu Thr Ile Asp Phe Gly Glu Lys Leu Ile Ser Thr Ser 
            100                 105                 110         


Glu Ala Gln Ala Gly Phe Lys Val Lys Leu Asp Phe Thr Gly Trp Arg 
        115                 120                 125             


Ala Val Gly Val Ser Leu Asn Asn Asp Leu Glu Asn Arg Glu Met Thr 
    130                 135                 140                 


Leu Asn Ala Thr Asn Thr Ser Ser Asp Gly Thr Gln Asp Ser Ile Gly 
145                 150                 155                 160 


Arg Ser Leu Gly Ala Lys Val Asp Ser Ile Arg Phe Lys Ala Pro Ser 
                165                 170                 175     


Asn Val Ser Gln Gly Glu Ile Tyr Ile Asp Arg Ile Met Phe Ser Val 
            180                 185                 190         


Asp Asp Ala Arg Tyr Gln Trp Ser Asp Tyr Gln Val Lys Thr Arg Leu 
        195                 200                 205             


Ser Glu Pro Glu Ile Gln Phe His Asn Val Lys Pro Gln Leu Pro Val 
    210                 215                 220                 


Thr Pro Glu Asn Leu Ala Ala Ile Asp Leu Ile Arg Gln Arg Leu Ile 
225                 230                 235                 240 


Asn Glu Phe Val Gly Gly Glu Lys Glu Thr Asn Leu Ala Leu Glu Glu 
                245                 250                 255     


Asn Ile Ser Lys Leu Lys Ser Asp Phe Asp Ala Leu Asn Ile His Thr 
            260                 265                 270         


Leu Ala Asn Gly Gly Thr Gln Gly Arg His Leu Ile Thr Asp Lys Gln 
        275                 280                 285             


Ile Ile Ile Tyr Gln Pro Glu Asn Leu Asn Ser Gln Asp Lys Gln Leu 
    290                 295                 300                 


Phe Asp Asn Tyr Val Ile Leu Gly Asn Tyr Thr Thr Leu Met Phe Asn 
305                 310                 315                 320 


Ile Ser Arg Ala Tyr Val Leu Glu Lys Asp Pro Thr Gln Lys Ala Gln 
                325                 330                 335     


Leu Lys Gln Met Tyr Leu Leu Met Thr Lys His Leu Leu Asp Gln Gly 
            340                 345                 350         


Phe Val Lys Gly Ser Ala Leu Val Thr Thr His His Trp Gly Tyr Ser 
        355                 360                 365             


Ser Arg Trp Trp Tyr Ile Ser Thr Leu Leu Met Ser Asp Ala Leu Lys 
    370                 375                 380                 


Glu Ala Asn Leu Gln Thr Gln Val Tyr Asp Ser Leu Leu Trp Tyr Ser 
385                 390                 395                 400 


Arg Glu Phe Lys Ser Ser Phe Asp Met Lys Val Ser Ala Asp Ser Ser 
                405                 410                 415     


Asp Leu Asp Tyr Phe Asn Thr Leu Ser Arg Gln His Leu Ala Leu Leu 
            420                 425                 430         


Leu Leu Glu Pro Asp Asp Gln Lys Arg Ile Asn Leu Val Asn Thr Phe 
        435                 440                 445             


Ser His Tyr Ile Thr Gly Ala Leu Thr Gln Val Pro Pro Gly Gly Lys 
    450                 455                 460                 


Asp Gly Leu Arg Pro Asp Gly Thr Ala Trp Arg His Glu Gly Asn Tyr 
465                 470                 475                 480 


Pro Gly Tyr Ser Phe Pro Ala Phe Lys Asn Ala Ser Gln Leu Ile Tyr 
                485                 490                 495     


Leu Leu Arg Asp Thr Pro Phe Ser Val Gly Glu Ser Gly Trp Asn Asn 
            500                 505                 510         


Leu Lys Lys Ala Met Val Ser Ala Trp Ile Tyr Ser Asn Pro Glu Val 
        515                 520                 525             


Gly Leu Pro Leu Ala Gly Arg His Pro Phe Asn Ser Pro Ser Leu Lys 
    530                 535                 540                 


Ser Val Ala Gln Gly Tyr Tyr Trp Leu Ala Met Ser Ala Lys Ser Ser 
545                 550                 555                 560 


Pro Asp Lys Thr Leu Ala Ser Ile Tyr Leu Ala Ile Ser Asp Lys Thr 
                565                 570                 575     


Gln Asn Glu Ser Thr Ala Ile Phe Gly Glu Thr Ile Thr Pro Ala Ser 
            580                 585                 590         


Leu Pro Gln Gly Phe Tyr Ala Phe Asn Gly Gly Ala Phe Gly Ile His 
        595                 600                 605             


Arg Trp Gln Asp Lys Met Val Thr Leu Lys Ala Tyr Asn Thr Asn Val 
    610                 615                 620                 


Trp Ser Ser Glu Ile Tyr Asn Lys Asp Asn Arg Tyr Gly Arg Tyr Gln 
625                 630                 635                 640 


Ser His Gly Val Ala Gln Ile Val Ser Asn Gly Ser Gln Leu Ser Gln 
                645                 650                 655     


Gly Tyr Gln Gln Glu Gly Trp Asp Trp Asn Arg Met Gln Gly Ala Thr 
            660                 665                 670         


Thr Ile His Leu Pro Leu Lys Asp Leu Asp Ser Pro Lys Pro His Thr 
        675                 680                 685             


Leu Met Gln Arg Gly Glu Arg Gly Phe Ser Gly Thr Ser Ser Leu Glu 
    690                 695                 700                 


Gly Gln Tyr Gly Met Met Ala Phe Asp Leu Ile Tyr Pro Ala Asn Leu 
705                 710                 715                 720 


Glu Arg Phe Asp Pro Asn Phe Thr Ala Lys Lys Ser Val Leu Ala Ala 
                725                 730                 735     


Asp Asn His Leu Ile Phe Ile Gly Ser Asn Ile Asn Ser Ser Asp Lys 
            740                 745                 750         


Asn Lys Asn Val Glu Thr Thr Leu Phe Gln His Ala Ile Thr Pro Thr 
        755                 760                 765             


Leu Asn Thr Leu Trp Ile Asn Gly Gln Lys Ile Glu Asn Met Pro Tyr 
    770                 775                 780                 


Gln Thr Thr Leu Gln Gln Gly Asp Trp Leu Ile Asp Ser Asn Gly Asn 
785                 790                 795                 800 


Gly Tyr Leu Ile Thr Gln Ala Glu Lys Val Asn Val Ser Arg Gln His 
                805                 810                 815     


Gln Val Ser Ala Glu Asn Lys Asn Arg Gln Pro Thr Glu Gly Asn Phe 
            820                 825                 830         


Ser Ser Ala Trp Ile Asp His Ser Thr Arg Pro Lys Asp Ala Ser Tyr 
        835                 840                 845             


Glu Tyr Met Val Phe Leu Asp Ala Thr Pro Glu Lys Met Gly Glu Met 
    850                 855                 860                 


Ala Gln Lys Phe Arg Glu Asn Asn Gly Leu Tyr Gln Val Leu Arg Lys 
865                 870                 875                 880 


Asp Lys Asp Val His Ile Ile Leu Asp Lys Leu Ser Asn Val Thr Gly 
                885                 890                 895     


Tyr Ala Phe Tyr Gln Pro Ala Ser Ile Glu Asp Lys Trp Ile Lys Lys 
            900                 905                 910         


Val Asn Lys Pro Ala Ile Val Met Thr His Arg Gln Lys Asp Thr Leu 
        915                 920                 925             


Ile Val Ser Ala Val Thr Pro Asp Leu Asn Met Thr Arg Gln Lys Ala 
    930                 935                 940                 


Ala Thr Pro Val Thr Ile Asn Val Thr Ile Asn Gly Lys Trp Gln Ser 
945                 950                 955                 960 


Ala Asp Lys Asn Ser Glu Val Lys Tyr Gln Val Ser Gly Asp Asn Thr 
                965                 970                 975     


Glu Leu Thr Phe Thr Ser Tyr Phe Gly Ile Pro Gln Glu Ile Lys Leu 
            980                 985                 990         


Ser Pro Leu Pro 
        995     


