                         SEQUENCE LISTING

<110>  Eberhard-Karls-Universitt Tbingen, Universittsklinikum
 
<120>  Permanent gene correction by means of nucleotide-modified 
       messenger RNA

<130>  5402P512WO

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  1
gtaggcgtgt acggtgggag                                                   20


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  2
cagcagaggg taggaagcag c                                                 21


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  3
tgtacggtgg gaggcctat                                                    19


<210>  4
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  4
cctggcaggt gatgtgg                                                      17


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  5
gcaagtttgg cgtcgctcca                                                   20


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  6
agaggaaggc gcggcagg                                                     18


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  7
ttcttgctcc agtgactctc tta                                               23


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  8
agcctagtaa agacaacact agtg                                              24


<210>  9
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  9
caacgtgacc tgcgagcg                                                     18


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  10
gtgcacgctc cacttctcg                                                    19


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  11
ctggagatgc atccttgtct gt                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  12
gagggtgaag acttttggag ct                                                22


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  13
cagcaccaga tgttccctgt ta                                                22


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  14
tggaaagcaa tagttctagg atga                                              24


<210>  15
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  15
cctggcaggt gatgtgg                                                      17


<210>  16
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  16
tgtacggtgg gaggcctat                                                    19


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  17
aggcactggg caggtaagta                                                   20


<210>  18
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  18
gtaaaacgac ggccagtg                                                     18


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  19
caggaaacag ctatgaccat g                                                 21


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  20
tgtacggcta caggggaa                                                     18


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  21
gccgataggc agattgta                                                     18


<210>  22
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  22
taggcaccag ggtgatg                                                      17


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  23
gccatgttca atggggtact                                                   20


<210>  24
<211>  1665
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transgenic SP-B cassette (before gene manipulation)


<220>
<221>  misc_feature
<222>  (1628)..(1632)

<220>
<221>  misc_feature
<222>  (1628)..(1632)
<223>  n is a, c, g, or t

<400>  24
cctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc       60

ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa      120

agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac      180

cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata      240

gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga      300

gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt      360

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga      420

caccgggacc gatccagcct ccgcggcccc gaattctgca gatatccagc acagtggcgg      480

ccgctaggca gccatggcca agtcgcacct actgcagtgg ctactgctgc ttcctaccct      540

ctgctgccca ggtgcagcta tcacgtcggc ctcatccctg gagtgtgcac aaggccctca      600

attctggtgc caaagcctgg agcatgcagt gcagtgcaga gccctggggc actgcctgca      660

ggaagtctgg gggcatgcag gagctaatga cctgtgccaa gagtgtgagg atattgtcca      720

cctcctcaca aagatgacca aggaagatgc tttccaggaa gcaatccgga agttcctgga      780

acaagaatgt gatatccttc ccttgaagct gcttgtgccc cggtgtcgcc aagtgcttga      840

tgtctacctg cccctggtta ttgactactt ccagagccag attaacccca aagccatctg      900

caatcatgtg ggcctgtgcc cacgtgggca ggctaagcca gaacagaatc cagggatgcc      960

ggatgccgtt ccaaaccctc tgctggacaa gctggtcctc cctgtgctgc caggagccct     1020

cttggcaagg cctgggcctc acactcagga cttctctgag caacagctcc ccattcccct     1080

gcccttctgc tggctttgca gaactctgat caagcgggtt caagccgtga tccccaaggg     1140

tgtgctggct gtggctgtgt cccaggtgtg ccacgtggta cccctggtgg tgggtggcat     1200

ctgccagtgc ctggctgagc gctacacagt tctcctgcta gacgcactgc tgggccgtgt     1260

ggtgccccag ctagtctgtg gccttgtcct ccgatgttcc actgaggatg ccatgggccc     1320

tgccctccct gctgtggagc ctctgataga agaatggcca ctacaggaca ctgagtgcca     1380

tttctgcaag tctgtgatca accaggcctg gaacaccagt gaacaggcta tgccacaggc     1440

aatgcaccag gcctgccttc gcttctggct agacaggcaa aagtgtgaac agtttgtgga     1500

acagcacatg ccccagctgc tggccctggt gcctaggagc caggatgccc acatcacctg     1560

ccaggccctt ggcgtatgtg aggccccggc tagccctctg cagtcgttcc aaaccccaca     1620

cctctgannn nntctagagg gcccgtttaa acccgctgat cagcc                     1665


<210>  25
<211>  2640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transgenic SP-B cassette (after gene manipulation)


<220>
<221>  misc_feature
<222>  (2603)..(2607)

<220>
<221>  misc_feature
<222>  (2603)..(2607)
<223>  n is a, c, g, or t

<400>  25
cctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc       60

ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa      120

agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac      180

cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata      240

gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga      300

gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt      360

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga      420

caccgggcta gcggatcctc tagaactata gctagtcgac attgattatt gactagttat      480

taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca      540

taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca      600

ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg      660

gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg      720

ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc      780

ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgtcg      840

aggccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt      900

tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggc gcgcgccagg      960

cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa     1020

tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta     1080

taaaaagcga agcgcgcggc gggcgggagc aagctttatt gcggtagttt atcacagtta     1140

aattgctaac gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg     1200

gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata     1260

gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct     1320

tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc     1380

ttaaggctag agtacttaat acgactcact ataggctagc ctcgagaatt ctgcagatat     1440

ccagcacagt ggcggccgct aggcagccat ggccaagtcg cacctactgc agtggctact     1500

gctgcttcct accctctgct gcccaggtgc agctatcacg tcggcctcat ccctggagtg     1560

tgcacaaggc cctcaattct ggtgccaaag cctggagcat gcagtgcagt gcagagccct     1620

ggggcactgc ctgcaggaag tctgggggca tgcaggagct aatgacctgt gccaagagtg     1680

tgaggatatt gtccacctcc tcacaaagat gaccaaggaa gatgctttcc aggaagcaat     1740

ccggaagttc ctggaacaag aatgtgatat ccttcccttg aagctgcttg tgccccggtg     1800

tcgccaagtg cttgatgtct acctgcccct ggttattgac tacttccaga gccagattaa     1860

ccccaaagcc atctgcaatc atgtgggcct gtgcccacgt gggcaggcta agccagaaca     1920

gaatccaggg atgccggatg ccgttccaaa ccctctgctg gacaagctgg tcctccctgt     1980

gctgccagga gccctcttgg caaggcctgg gcctcacact caggacttct ctgagcaaca     2040

gctccccatt cccctgccct tctgctggct ttgcagaact ctgatcaagc gggttcaagc     2100

cgtgatcccc aagggtgtgc tggctgtggc tgtgtcccag gtgtgccacg tggtacccct     2160

ggtggtgggt ggcatctgcc agtgcctggc tgagcgctac acagttctcc tgctagacgc     2220

actgctgggc cgtgtggtgc cccagctagt ctgtggcctt gtcctccgat gttccactga     2280

ggatgccatg ggccctgccc tccctgctgt ggagcctctg atagaagaat ggccactaca     2340

ggacactgag tgccatttct gcaagtctgt gatcaaccag gcctggaaca ccagtgaaca     2400

ggctatgcca caggcaatgc accaggcctg ccttcgcttc tggctagaca ggcaaaagtg     2460

tgaacagttt gtggaacagc acatgcccca gctgctggcc ctggtgccta ggagccagga     2520

tgcccacatc acctgccagg cccttggcgt atgtgaggcc ccggctagcc ctctgcagtc     2580

gttccaaacc ccacacctct gannnnntct agagggcccg tttaaacccg ctgatcagcc     2640


<210>  26
<211>  6122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVAX1 A120 TALEN 1 LEFT

<400>  26
gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga      660

aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt      720

accgagctcg gatccactag tccagtgtgg tggaattagc tctctggcta actagagaac      780

ccactgctta ctggcttatc gaaattaata cgactcacta tagggagagc caagctgact      840

agcgtttaaa cttaagctga tccactagtc cagtgtggtg gaattcgcca tggactacaa      900

agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat      960

ggcccccaag aagaagagga aggtcggcat ccacggggta cctatggtgg acttgaggac     1020

actcggttat tcgcaacagc aacaggagaa aatcaagcct aaggtcagga gcaccgtcgc     1080

gcaacaccac gaggcgcttg tggggcatgg cttcactcat gcgcatattg tcgcgctttc     1140

acagcaccct gcggcgcttg ggacggtggc tgtcaaatac caagatatga ttgcggccct     1200

gcccgaagcc acgcacgagg caattgtagg ggtcggtaaa cagtggtcgg gagcgcgagc     1260

acttgaggcg ctgctgactg tggcgggtga gcttaggggg cctccgctcc agctcgacac     1320

cgggcagctg ctgaagatcg cgaagagagg gggagtaaca gcggtagagg cagtgcacgc     1380

ctggcgcaat gcgctcaccg gggccccctt gaacctgacc ccagaccagg tagtcgcaat     1440

cgcgtcgaat ggcgggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtcct     1500

ttgtcaagac cacggcctta caccggatca agtcgtggcc attgcaaata ataacggtgg     1560

caaacaggct cttgagacgg ttcagagact tctcccagtt ctctgtcaag cccacgggct     1620

gactcccgat caagttgtag cgattgcgag caacatcgga gggaaacaag cattggagac     1680

tgtccaacgg ctccttcccg tgttgtgtca agcccacggt ttgacgcctg cacaagtggt     1740

cgccatcgcc tcccacgacg gcggtaagca ggcgctggaa acagtacagc gcctgctgcc     1800

tgtactgtgc caggatcatg gactcacccc agaccaggta gtcgcaatcg cgtcgcatga     1860

cgggggaaag caagccctgg aaaccgtgca aaggttgttg ccggtccttt gtcaagacca     1920

cggccttaca ccggagcaag tcgtggccat tgcatcaaac ggaggtggca aacaggctct     1980

tgagacggtt cagagacttc tcccagttct ctgtcaagcc cacgggctga ctcccgatca     2040

agttgtagcg attgcgagcc atgatggagg gaaacaagca ttggagactg tccaacggct     2100

ccttcccgtg ttgtgtcaag cccacggttt gacgcctgca caagtggtcg ccatcgactc     2160

ccacgacggc ggtaagcagg cgctggaaac agtacagcgc ctgctgcctg tactgtgcca     2220

ggatcatggg ctgaccccag accaggtagt cgcaatcgcg tcgaacattg ggggaaagca     2280

agccctggaa accgtgcaaa ggttgttgcc ggtcctttgt caagaccacg gccttacacc     2340

ggagcaagtc gtggccattg catcaaacgg aggtggcaaa caggctcttg agacggttca     2400

gagacttctc ccagttctct gtcaagccca cgggctgact cccgatcaag ttgtagcgat     2460

tgcgagcaac atcggaggga aacaagcatt ggagactgtc caacggctcc ttcccgtgtt     2520

gtgtcaagcc cacggtttga cgcctgcaca agtggtcgcc atcgccaaca acaacggcgg     2580

taagcaggcg ctggaaacag tacagcgcct gctgcctgta ctgtgccagg atcatggttt     2640

gaccccagac caggtagtcg caatcgcgtc gaacattggg ggaaagcaag ccctggaaac     2700

cgtgcaaagg ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt     2760

ggccattgca tcaaatatcg gtggcaaaca ggctcttgag acggttcaga gacttctccc     2820

agttctctgt caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgaataacaa     2880

tggagggaaa caagcattgg agactgtcca acggctcctt cccgtgttgt gtcaagccca     2940

cggtttgacg cctgcacaag tggtcgccat cgcctccaat attggcggta agcaggcgct     3000

ggaaacagta cagcgcctgc tgcctgtact gtgccaggat catggcctga cacccgaaca     3060

ggtggtcgcc attgctagcc acgatggagg acggccagcc ttggagtcca tcgtagccca     3120

attgtccagg cccgatcccg cgttggctgc gttaacggga tcccagctgg tgaagagcga     3180

gctggaggag aagaagtccg agctgcggca caagctgaag tacgtgcccc acgagtacat     3240

cgagctgatc gagatcgcca ggaacagcac ccaggaccgc atcctggaga tgaaggtgat     3300

ggagttcttc atgaaggtgt acggctacag gggaaagcac ctgggcggaa gcagaaagcc     3360

tgacggcgcc atctatacag tgggcagccc catcgattac ggcgtgatcg tggacacaaa     3420

ggcctacagc ggcggctaca atctgcctat cggccaggcc gacgagatgg agagatacgt     3480

ggaggagaac cagacccggg ataagcacct caaccccaac gagtggtgga aggtgtaccc     3540

tagcagcgtg accgagttca agttcctgtt cgtgagcggc cacttcaagg gcaactacaa     3600

ggcccagctg accaggctga accacatcac caactgcaat ggcgccgtgc tgagcgtgga     3660

ggagctgctg atcggcggcg agatgatcaa agccggcacc ctgacactgg aggaggtgcg     3720

gcgcaagttc aacaacggcg agatcaactt cagatcttga taactcgagc taattctgca     3780

gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3900

agcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt     3960

ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg     4020

ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt     4080

gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca     4140

atagcaggca tgctggggat gcggtgggct ctatggcttc tactgggcgg ttttatggac     4200

agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa     4260

agtaaactgg atggctttct cgccgccaag gatctgatgg cgcaggggat caagctctga     4320

tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc     4380

tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg     4440

ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac     4500

cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc     4560

cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg     4620

gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga     4680

gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg     4740

cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg     4800

tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt     4860

cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc     4920

ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg     4980

gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga     5040

gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc     5100

gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattatta acgcttacaa     5160

tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacaggt     5220

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     5280

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata gcacgtgcta     5340

aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc     5400

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     5460

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     5520

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     5580

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     5640

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     5700

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     5760

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     5820

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     5880

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     5940

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     6000

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     6060

gccagcaacg cggccttttt acggttcctg ggcttttgct ggccttttgc tcacatgttc     6120

tt                                                                    6122


<210>  27
<211>  6194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVAX1 A120 TALEN 1 RIGHT

<400>  27
gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga      660

aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt      720

accgagctcg gatccactag tccagtgtgg tggaattagc tctctggcta actagagaac      780

ccactgctta ctggcttatc gaaattaata cgactcacta tagggagagc caagctgact      840

agcgtttaaa cttaagctga tccactagtc cagtgtggtg gaattcgcct agagatctgg      900

cggcggagag ggcagaggaa gtcttctaac ctgcggtgac gtggaggaga atcccggccc      960

taggaccatg gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa     1020

ggatgacgat gacaagatgg cccccaagaa gaagaggaag gtcggcattc atggggtacc     1080

tatggtggac ttgaggacac tcggttattc gcaacagcaa caggagaaaa tcaagcctaa     1140

ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg gggcatggct tcactcatgc     1200

gcatattgtc gcgctttcac agcaccctgc ggcgcttggg acggtggctg tcaaatacca     1260

agatatgatt gcggccctgc ccgaagccac gcacgaggca attgtagggg tcggtaaaca     1320

gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc ttagggggcc     1380

tccgctccag ctcgacaccg ggcagctgct gaagatcgcg aagagagggg gagtaacagc     1440

ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga acctgacccc     1500

agaccaggta gtcgcaatcg cgtcgcatga cgggggaaag caagccctgg aaaccgtgca     1560

aaggttgttg ccggtccttt gtcaagacca cggccttaca ccggatcaag tcgtggccat     1620

tgcaaataat aacggtggca aacaggctct tgagacggtt cagagacttc tcccagttct     1680

ctgtcaagcc cacgggctga ctcccgatca agttgtagcg attgcgaata acaatggagg     1740

gaaacaagca ttggagactg tccaacggct ccttcccgtg ttgtgtcaag cccacggttt     1800

gacgcctgca caagtggtcg ccatcgccaa caacaacggc ggtaagcagg cgctggaaac     1860

agtacagcgc ctgctgcctg tactgtgcca ggatcatgga ctcaccccag accaggtagt     1920

cgcaatcgcc aacaataacg ggggaaagca agccctggaa accgtgcaaa ggttgttgcc     1980

ggtcctttgt caagaccacg gccttacacc ggagcaagtc gtggccattg catcacatga     2040

cggtggcaaa caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca     2100

cgggctgact cccgatcaag ttgtagcgat tgcgagccat gatggaggga aacaagcatt     2160

ggagactgtc caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca     2220

agtggtcgcc atcgccaaca acaacggcgg taagcaggcg ctggaaacag tacagcgcct     2280

gctgcctgta ctgtgccagg atcatgggct gaccccagac caggtagtcg caatcgcgtc     2340

gcatgacggg ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg tcctttgtca     2400

agaccacggc cttacaccgg atcaagtcgt ggccattgca aataataacg gtggcaaaca     2460

ggctcttgag acggttcaga gacttctccc agttctctgt caagcccacg ggctgactcc     2520

cgatcaagtt gtagcgattg cgaataacaa tggagggaaa caagcattgg agactgtcca     2580

acggctcctt cccgtgttgt gtcaagccca cggtttgacg cctgcacaag tggtcgccat     2640

cgcctccaat attggcggta agcaggcgct ggaaacagta cagcgcctgc tgcctgtact     2700

gtgccaggat catggtttga ccccagacca ggtagtcgca atcgccaaca ataacggggg     2760

aaagcaagcc ctggaaaccg tgcaaaggtt gttgcaggtc ctttgtcaag accacggcct     2820

tacaccggat caagtcgtgg ccattgcaaa taataacggt ggcaaacagg ctcttgagac     2880

ggttcagaga cttctcccag ttctctgtca agcccacggg ctgactcccg atcaagttgt     2940

agcgattgcg agccatgatg gagggaaaca agcattggag actgtccaac ggctccttcc     3000

cgtgttgtgt caagcccacg gtttgacgcc tgcacaagtg gtcgccatcg cctccaacgg     3060

tggcggtaag caggcgctgg aaacagtaca gcgcctgctg cctgtactgt gccaggatca     3120

tggcctgaca cccgaacagg tggtcgccat tgctagcaat aaaggaggac ggccagcctt     3180

ggagtccatc gtagcccaat tgtccaggcc cgatcccgcg ttggctgcgt taacgggatc     3240

ccagctggtg aagagcgagc tggaggagaa gaagtccgag ctgcggcaca agctgaagta     3300

cgtgccccac gagtacatcg agctgatcga gatcgccagg aacagcaccc aggaccgcat     3360

cctggagatg aaggtgatgg agttcttcat gaaggtgtac ggctacaggg gaaagcacct     3420

gggcggaagc agaaagcctg acggcgccat ctatacagtg ggcagcccca tcgattacgg     3480

cgtgatcgtg gacacaaagg cctacagcgg cggctacaat ctgcctatcg gccaggccga     3540

cgagatgcag agatacgtga aggagaacca gacccggaat aagcacatca accccaacga     3600

gtggtggaag gtgtacccta gcagcgtgac cgagttcaag ttcctgttcg tgagcggcca     3660

cttcaagggc aactacaagg cccagctgac caggctgaac cgcaaaacca actgcaatgg     3720

cgccgtgctg agcgtggagg agctgctgat cggcggcgag atgatcaaag ccggcaccct     3780

gacactggag gaggtgcggc gcaagttcaa caacggcgag atcaacttct gataactcga     3840

gctaattctg cagaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3960

aaaaaaaaaa aaagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct     4020

cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga     4080

ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt     4140

gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg     4200

attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctactgggc     4260

ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg     4320

gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg     4380

atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt     4440

gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca     4500

gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct     4560

ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct     4620

atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc     4680

gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct     4740

tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga     4800

tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg     4860

gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc     4920

agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac     4980

ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat     5040

cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga     5100

tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc     5160

cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat     5220

taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca     5280

ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc     5340

taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa     5400

tagcacgtgc taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat     5460

aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     5520

gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     5580

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     5640

tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag     5700

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     5760

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     5820

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     5880

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     5940

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     6000

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     6060

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     6120

ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tgggcttttg ctggcctttt     6180

gctcacatgt tctt                                                       6194


<210>  28
<211>  4354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVAX1 A120 ZFN 3 LEFT

<400>  28
gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga      660

aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt      720

accgagctcg gatccactag tccagtgtgg tggaattaat tcgcctagag atctggcggc      780

ggagagggca gaggaagtct tctaacctgc ggtgacgtgg aggagaatcc cggccctagg      840

accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat      900

gacgatgaca agatggcccc caagaagaag aggaaggtcg gcattcatgg ggtacccgcc      960

gctatggctg agaggccctt ccagtgtcga atctgcatgc gtaacttcag tgaccagtcc     1020

aacctgcgcg cccacatccg cacccacacc ggcgagaagc cttttgcctg tgacatttgt     1080

gggaggaaat ttgcccgcaa gtccgaccgc atcaagcata ccaagataca cacgggcagc     1140

caaaagccct tccagtgtcg aatctgcatg cgtaagtttg cccgctccga caacctgtcc     1200

gtgcatacca agatacacac gggcgagaag cccttccagt gtcgaatctg catgcgtaac     1260

ttcagtgagc gcggcaccct ggcccgccac atccgcaccc acaccggcga gaagcctttt     1320

gcctgtgaca tttgtgggag gaaatttgcc cgctccgacg ccctgaccca gcataccaag     1380

atacacctgc ggggatccca gctggtgaag agcgagctgg aggagaagaa gtccgagctg     1440

cggcacaagc tgaagtacgt gccccacgag tacatcgagc tgatcgagat cgccaggaac     1500

agcacccagg accgcatcct ggagatgaag gtgatggagt tcttcatgaa ggtgtacggc     1560

tacaggggaa agcacctggg cggaagcaga aagcctgacg gcgccatcta tacagtgggc     1620

agccccatcg attacggcgt gatcgtggac acaaaggcct acagcggcgg ctacaatctg     1680

cctatcggcc aggccgacga gatgcagaga tacgtgaagg agaaccagac ccggaataag     1740

cacatcaacc ccaacgagtg gtggaaggtg taccctagca gcgtgaccga gttcaagttc     1800

ctgttcgtga gcggccactt caagggcaac tacaaggccc agctgaccag gctgaaccgc     1860

aaaaccaact gcaatggcgc cgtgctgagc gtggaggagc tgctgatcgg cggcgagatg     1920

atcaaagccg gcaccctgac actggaggag gtgcggcgca agttcaacaa cggcgagatc     1980

aacttctgat aactcgagtc tagaattctg cagaaaaaaa aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagcggccg ctcgagtcta gagggcccgt     2160

ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc     2220

ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa     2280

tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg     2340

gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg     2400

ctctatggct tctactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg     2460

gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca     2520

aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc     2580

atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc     2640

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     2700

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     2760

caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     2820

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     2880

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     2940

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     3000

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     3060

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     3120

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     3180

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     3240

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     3300

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     3360

gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg     3420

catctgtgcg gtatttcaca ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa     3480

cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac     3540

cctgataaat gcttcaataa tagcacgtgc taaaacttca tttttaattt aaaaggatct     3600

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc     3660

actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc     3720

gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg     3780

atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa     3840

atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc     3900

ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt     3960

gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa     4020

cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc     4080

tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc     4140

cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct     4200

ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat     4260

gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc     4320

tgggcttttg ctggcctttt gctcacatgt tctt                                 4354


<210>  29
<211>  4393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVAX1 A120 ZFN 3 RIGHT

<400>  29
gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga      660

aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt      720

accgagctcg gatccactag tccagtgtgg tggaattaat tcgccatgga ctacaaagac      780

catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagatggcc      840

cccaagaaga agaggaaggt cggcatccac ggggtacccg ccgctatggc tgagaggccc      900

ttccagtgtc gaatctgcat gcgtaacttc agtcagtcct ccgacctgtc ccgccacatc      960

cgcacccaca ccggcgagaa gccttttgcc tgtgacattt gtgggaggaa atttgcctgg     1020

cgctcctccc tgcgccagca taccaagata cacacgcatc ccagggcacc tattcccaag     1080

cccttccagt gtcgaatctg catgcgtaac ttcagtcagt ccggcgacct gacccgccac     1140

atccgcaccc acaccggcga gaagcctttt gcctgtgaca tttgtgggag gaaatttgcc     1200

cgccgcgccg accgcgccaa gcataccaag atacacacgc acccgcgcgc cccgatcccg     1260

aagcccttcc agtgtcgaat ctgcatgcgt aacttcagtc gctccgacga cctgacccgc     1320

cacatccgca cccacaccgg cgagaagcct tttgcctgtg acatttgtgg gaggaaattt     1380

gcccagcgct ccaccctgtc ctcccatacc aagatacacc tgcggggatc ccagctggtg     1440

aagagcgagc tggaggagaa gaagtccgag ctgcggcaca agctgaagta cgtgccccac     1500

gagtacatcg agctgatcga gatcgccagg aacagcaccc aggaccgcat cctggagatg     1560

aaggtgatgg agttcttcat gaaggtgtac ggctacaggg gaaagcacct gggcggaagc     1620

agaaagcctg acggcgccat ctatacagtg ggcagcccca tcgattacgg cgtgatcgtg     1680

gacacaaagg cctacagcgg cggctacaat ctgcctatcg gccaggccga cgagatggag     1740

agatacgtgg aggagaacca gacccgggat aagcacctca accccaacga gtggtggaag     1800

gtgtacccta gcagcgtgac cgagttcaag ttcctgttcg tgagcggcca cttcaagggc     1860

aactacaagg cccagctgac caggctgaac cacatcacca actgcaatgg cgccgtgctg     1920

agcgtggagg agctgctgat cggcggcgag atgatcaaag ccggcaccct gacactggag     1980

gaggtgcggc gcaagttcaa caacggcgag atcaacttca gatcttgata actcgagtct     2040

agaattctgc agaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2100

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2160

aaaaaaaaaa aagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc     2220

gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac     2280

cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg     2340

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga     2400

ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctactgggcg     2460

gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg     2520

aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg gcgcagggga     2580

tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg     2640

cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag     2700

acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt     2760

tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta     2820

tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg     2880

ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt     2940

gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat     3000

ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg     3060

atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca     3120

gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc     3180

catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc     3240

gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat     3300

attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc     3360

gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattatt     3420

aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac     3480

cgcatacagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     3540

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     3600

agcacgtgct aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     3660

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     3720

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     3780

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     3840

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc     3900

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     3960

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     4020

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     4080

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     4140

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     4200

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     4260

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     4320

tatggaaaaa cgccagcaac gcggcctttt tacggttcct gggcttttgc tggccttttg     4380

ctcacatgtt ctt                                                        4393


<210>  30
<211>  4541
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pVAX1 A120 Foxp3 (for mock mRNA)

<400>  30
gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga      660

aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt      720

acctcagacg agacttggaa gacagtcaca tctcagcagc tcctctgccg ttatccagcc      780

tgcctctgac aagaacccaa tgcccaaccc taggccagcc aagcctatgg ctccttcctt      840

ggcccttggc ccatccccag gagtcttgcc aagctggaag actgcaccca agggctcaga      900

acttctaggg accaggggct ctgggggacc cttccaaggt cgggacctgc gaagtggggc      960

ccacacctct tcttccttga accccctgcc accatcccag ctgcagctgc ctacagtgcc     1020

cctagtcatg gtggcaccgt ctggggcccg actaggtccc tcaccccacc tacaggccct     1080

tctccaggac agaccacact tcatgcatca gctctccact gtggatgccc atgcccagac     1140

ccctgtgctc caagtgcgtc cactggacaa cccagccatg atcagcctcc caccaccttc     1200

tgctgccact ggggtcttct ccctcaaggc ccggcctggc ctgccacctg ggatcaatgt     1260

ggccagtctg gaatgggtgt ccagggagcc agctctactc tgcaccttcc cacgctcggg     1320

tacacccagg aaagacagca accttttggc tgcaccccaa ggatcctacc cactgctggc     1380

aaatggagtc tgcaagtggc ctggttgtga gaaggtcttc gaggagccag aagagtttct     1440

caagcactgc caagcagatc atctcctgga tgagaaaggc aaggcccagt gcctcctcca     1500

gagagaagtg gtgcagtctc tggagcagca gctggagctg gaaaaggaga agctgggagc     1560

tatgcaggcc cacctggctg ggaagatggc gctggccaag gctccatctg tggcctcaat     1620

ggacaagagc tcttgctgca tcgtagccac cagtactcag ggcagtgtgc tcccggcctg     1680

gtctgctcct cgggaggctc cagacggcgg cctgtttgca gtgcggaggc acctctgggg     1740

aagccatggc aatagttcct tcccagagtt cttccacaac atggactact tcaagtacca     1800

caatatgcga ccccctttca cctatgccac ccttatccga tgggccatcc tggaagcccc     1860

ggagaggcag aggacactca atgaaatcta ccattggttt actcgcatgt tcgcctactt     1920

cagaaaccac cccgccacct ggaagaatgc catccgccac aacctgagcc tgcacaagtg     1980

ctttgtgcga gtggagagcg agaagggagc agtgtggacc gtagatgaat ttgagtttcg     2040

caagaagagg agccaacgcc ccaacaagtg ctccaatccc tgcccttgac ctcaaaacca     2100

agaaaaggtg ggcgggggag ggggccaaaa ccatgagact gaggctgtgg gggcaaggag     2160

gcaagtccta cgtgtaccta tggaaaccgg aattctgcag aaaaaaaaaa aaaaaaaaaa     2220

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     2280

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gcggccgctc gagtctagag     2340

ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg     2400

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct     2460

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg     2520

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg     2580

cggtgggctc tatggcttct actgggcggt tttatggaca gcaagcgaac cggaattgcc     2640

agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctc     2700

gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc     2760

gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag     2820

gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg     2880

gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa     2940

tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc     3000

agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc     3060

ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga     3120

tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa     3180

acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct     3240

ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat     3300

gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt     3360

ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta     3420

tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga     3480

ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg     3540

ccttcttgac gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct     3600

ccttacgcat ctgtgcggta tttcacaccg catacaggtg gcacttttcg gggaaatgtg     3660

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga     3720

caataaccct gataaatgct tcaataatag cacgtgctaa aacttcattt ttaatttaaa     3780

aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt     3840

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt     3900

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt     3960

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag     4020

ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta     4080

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat     4140

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg     4200

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg     4260

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac     4320

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga     4380

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt     4440

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta     4500

cggttcctgg gcttttgctg gccttttgct cacatgttct t                         4541


<210>  31
<211>  6612
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV6_CB7_Cl-Foxp3 (mock-control)

<400>  31
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt       60

tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac      120

taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacca gggtaatggg      180

gatcctctag aactatagct agtcgacatt gattattgac tagttattaa tagtaatcaa      240

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa      300

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg      360

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggac tatttacggt      420

aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg      480

tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc      540

ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca      600

cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta      660

ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg      720

gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc      780

agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata      840

aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg      900

ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt      960

gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct     1020

tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg     1080

ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc     1140

cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag     1200

tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg     1260

gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc     1320

ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc     1380

gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg     1440

gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg     1500

gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct     1560

tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc     1620

gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc     1680

cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct     1740

ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag     1800

ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca     1860

tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca     1920

ttttggcaaa gaattcacgc gtggtacctc agacgagact tggaagacag tcacatctca     1980

gcagctcctc tgccgttatc cagcctgcct ctgacaagaa cccaatgccc aaccctaggc     2040

cagccaagcc tatggctcct tccttggccc ttggcccatc cccaggagtc ttgccaagct     2100

ggaagactgc acccaagggc tcagaacttc tagggaccag gggctctggg ggacccttcc     2160

aaggtcggga cctgcgaagt ggggcccaca cctcttcttc cttgaacccc ctgccaccat     2220

cccagctgca gctgcctaca gtgcccctag tcatggtggc accgtctggg gcccgactag     2280

gtccctcacc ccacctacag gcccttctcc aggacagacc acacttcatg catcagctct     2340

ccactgtgga tgcccatgcc cagacccctg tgctccaagt gcgtccactg gacaacccag     2400

ccatgatcag cctcccacca ccttctgctg ccactggggt cttctccctc aaggcccggc     2460

ctggcctgcc acctgggatc aatgtggcca gtctggaatg ggtgtccagg gagccagctc     2520

tactctgcac cttcccacgc tcgggtacac ccaggaaaga cagcaacctt ttggctgcac     2580

cccaaggatc ctacccactg ctggcaaatg gagtctgcaa gtggcctggt tgtgagaagg     2640

tcttcgagga gccagaagag tttctcaagc actgccaagc agatcatctc ctggatgaga     2700

aaggcaaggc ccagtgcctc ctccagagag aagtggtgca gtctctggag cagcagctgg     2760

agctggaaaa ggagaagctg ggagctatgc aggcccacct ggctgggaag atggcgctgg     2820

ccaaggctcc atctgtggcc tcaatggaca agagctcttg ctgcatcgta gccaccagta     2880

ctcagggcag tgtgctcccg gcctggtctg ctcctcggga ggctccagac ggcggcctgt     2940

ttgcagtgcg gaggcacctc tggggaagcc atggcaatag ttccttccca gagttcttcc     3000

acaacatgga ctacttcaag taccacaata tgcgaccccc tttcacctat gccaccctta     3060

tccgatgggc catcctggaa gccccggaga ggcagaggac actcaatgaa atctaccatt     3120

ggtttactcg catgttcgcc tacttcagaa accaccccgc cacctggaag aatgccatcc     3180

gccacaacct gagcctgcac aagtgctttg tgcgagtgga gagcgagaag ggagcagtgt     3240

ggaccgtaga tgaatttgag tttcgcaaga agaggagcca acgccccaac aagtgctcca     3300

atccctgccc ttgacctcaa aaccaagaaa aggtgggcgg gggagggggc caaaaccatg     3360

agactgaggc tgtgggggca aggaggcaag tcctacgtgt acctatggaa accgctcgag     3420

gacggggtga actacgcctg aggatccgat ctttttccct ctgccaaaaa ttatggggac     3480

atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca     3540

atagtgtgtt ggaatttttt gtgtctctca ctcggaagca attcgttgat ctgaatttcg     3600

accacccata atacccatta ccctggtaga taagtagcat ggcgggttaa tcattaacta     3660

caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga     3720

ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga     3780

gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg     3840

ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg     3900

gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg     3960

cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag     4020

cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt     4080

tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt     4140

ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg     4200

tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt     4260

taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt     4320

tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca     4380

aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg     4440

ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg     4500

ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt     4560

attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt     4620

gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg     4680

ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa     4740

cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt     4800

gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag     4860

tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt     4920

gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga     4980

ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt     5040

tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta     5100

gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg     5160

caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc     5220

cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt     5280

atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg     5340

gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg     5400

attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa     5460

cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     5520

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     5580

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     5640

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     5700

ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac     5760

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     5820

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     5880

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     5940

acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc     6000

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     6060

agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     6120

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc     6180

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     6240

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     6300

gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc     6360

ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac     6420

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact     6480

cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg     6540

agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa     6600

ggccttaatt ag                                                         6612


<210>  32
<211>  6898
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV6_CAG_SP-B_DONOR

<400>  32
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgca cgcgtcgtct cacatgtggc     4080

gcgccaacat gtctcgagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc     4140

gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga     4200

gtggccaact ccatcactag gggttcctgt gatagagaaa agtgaaagtc gagctcggta     4260

cccgggtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa     4320

ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga     4380

ccgatccagc ctccgcggcc ccgaattctg cagatatcca gcacagtggc ggccgctagg     4440

gctagcggat cctctagaac tatagctagt cgacattgat tattgactag ttattaatag     4500

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt     4560

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg     4620

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggactat     4680

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct     4740

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg     4800

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat gtcgaggcca     4860

cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta     4920

ttttttaatt attttgtgca gcgatggggg cggggggggg gggcgcgcgc caggcggggc     4980

ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag     5040

cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa     5100

gcgaagcgcg cggcgggcgg gagcaagctt tattgcggta gtttatcaca gttaaattgc     5160

taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagaa gttggtcgtg     5220

aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact     5280

gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga     5340

catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg     5400

ctagagtact taatacgact cactataggc tagcctcgag gccgccatgg ccaagtcgca     5460

cctactgcag tggctactgc tgcttcctac cctctgctgc ccaggtgcag ctatcacgtc     5520

ggcctcatcc ctggagtgtg cacaaggccc tcaattctgg tgccaaagcc tggagcatgc     5580

agtgcagtgc agagccctgg ggcactgcct gcaggaagtc tgggggcatg caggagctaa     5640

tgacctgtgc caagagtgtg aggatattgt ccacctcctc acaaagatga ccaaggaaga     5700

tgctttccag gaagcaatcc ggaagttcct ggaacaagaa tgtgatatcc ttcccttgaa     5760

gctgcttgtg ccccggtgtc gccaagtgct tgatgtctac ctgcccctgg ttattgacta     5820

cttccagagc cagattaacc ccaaagccat ctggaggttg aggattagag tccactagat     5880

ggggatacgc ggaacggtcg ggcgagtcat tagagtcctg gaaccggagg gtttaacgac     5940

ccaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga     6000

ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga     6060

gcgagcgcgc agccttaatt aatccggacc acgtgcggac cgagcggccg caggaacccc     6120

tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac     6180

caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca     6240

gctgcctgca ggaagctgta agcttgtcga gaagtactag aggatcataa tcagccatac     6300

cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa     6360

acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa     6420

ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg     6480

tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgatatcgcc     6540

taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt     6600

tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata     6660

atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg     6720

gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac     6780

cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt     6840

tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatg       6898


<210>  33
<211>  7026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV6-ZFN 3-LEFT

<400>  33
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa     4080

tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa     4140

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata     4200

atgacgtatg ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag     4260

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc     4320

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta     4380

tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg     4440

cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt     4500

ctccacccca ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa     4560

aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg     4620

tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa     4680

ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta agctgatcca     4740

ctagtccagt gtggtggaat tcgcctagag atctggcggc ggagagggca gaggaagtct     4800

tctaacctgc ggtgacgtgg aggagaatcc cggccctagg accatggact acaaagacca     4860

tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agatggcccc     4920

caagaagaag aggaaggtcg gcattcatgg ggtacccgcc gctatggctg agaggccctt     4980

ccagtgtcga atctgcatgc gtaacttcag tgaccagtcc aacctgcgcg cccacatccg     5040

cacccacacc ggcgagaagc cttttgcctg tgacatttgt gggaggaaat ttgcccgcaa     5100

gtccgaccgc atcaagcata ccaagataca cacgggcagc caaaagccct tccagtgtcg     5160

aatctgcatg cgtaagtttg cccgctccga caacctgtcc gtgcatacca agatacacac     5220

gggcgagaag cccttccagt gtcgaatctg catgcgtaac ttcagtgagc gcggcaccct     5280

ggcccgccac atccgcaccc acaccggcga gaagcctttt gcctgtgaca tttgtgggag     5340

gaaatttgcc cgctccgacg ccctgaccca gcataccaag atacacctgc ggggatccca     5400

gctggtgaag agcgagctgg aggagaagaa gtccgagctg cggcacaagc tgaagtacgt     5460

gccccacgag tacatcgagc tgatcgagat cgccaggaac agcacccagg accgcatcct     5520

ggagatgaag gtgatggagt tcttcatgaa ggtgtacggc tacaggggaa agcacctggg     5580

cggaagcaga aagcctgacg gcgccatcta tacagtgggc agccccatcg attacggcgt     5640

gatcgtggac acaaaggcct acagcggcgg ctacaatctg cctatcggcc aggccgacga     5700

gatgcagaga tacgtgaagg agaaccagac ccggaataag cacatcaacc ccaacgagtg     5760

gtggaaggtg taccctagca gcgtgaccga gttcaagttc ctgttcgtga gcggccactt     5820

caagggcaac tacaaggccc agctgaccag gctgaaccgc aaaaccaact gcaatggcgc     5880

cgtgctgagc gtggaggagc tgctgatcgg cggcgagatg atcaaagccg gcaccctgac     5940

actggaggag gtgcggcgca agttcaacaa cggcgagatc aacttctgat aactcgagct     6000

gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg     6060

gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg     6120

agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg     6180

gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcggaccg agcggccgca     6240

ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc     6300

cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg     6360

agcgcgcagc tgcctgcagg aagctgtaag cttgtcgaga agtactagag gatcataatc     6420

agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg     6480

aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat     6540

ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat     6600

tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg     6660

atatcgccta ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt     6720

tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc     6780

cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc     6840

ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg     6900

tgacaaaccg tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat     6960

ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg     7020

cgaatg                                                                7026


<210>  34
<211>  7065
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV6-ZFN 3-RIGHT

<400>  34
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa     4080

tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa     4140

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata     4200

atgacgtatg ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag     4260

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc     4320

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta     4380

tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg     4440

cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt     4500

ctccacccca ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa     4560

aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg     4620

tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa     4680

ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta agctgatcca     4740

ctagtccagt gtggtggaat tcgccatgga ctacaaagac catgacggtg attataaaga     4800

tcatgacatc gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt     4860

cggcatccac ggggtacccg ccgctatggc tgagaggccc ttccagtgtc gaatctgcat     4920

gcgtaacttc agtcagtcct ccgacctgtc ccgccacatc cgcacccaca ccggcgagaa     4980

gccttttgcc tgtgacattt gtgggaggaa atttgcctgg cgctcctccc tgcgccagca     5040

taccaagata cacacgcatc ccagggcacc tattcccaag cccttccagt gtcgaatctg     5100

catgcgtaac ttcagtcagt ccggcgacct gacccgccac atccgcaccc acaccggcga     5160

gaagcctttt gcctgtgaca tttgtgggag gaaatttgcc cgccgcgccg accgcgccaa     5220

gcataccaag atacacacgc acccgcgcgc cccgatcccg aagcccttcc agtgtcgaat     5280

ctgcatgcgt aacttcagtc gctccgacga cctgacccgc cacatccgca cccacaccgg     5340

cgagaagcct tttgcctgtg acatttgtgg gaggaaattt gcccagcgct ccaccctgtc     5400

ctcccatacc aagatacacc tgcggggatc ccagctggtg aagagcgagc tggaggagaa     5460

gaagtccgag ctgcggcaca agctgaagta cgtgccccac gagtacatcg agctgatcga     5520

gatcgccagg aacagcaccc aggaccgcat cctggagatg aaggtgatgg agttcttcat     5580

gaaggtgtac ggctacaggg gaaagcacct gggcggaagc agaaagcctg acggcgccat     5640

ctatacagtg ggcagcccca tcgattacgg cgtgatcgtg gacacaaagg cctacagcgg     5700

cggctacaat ctgcctatcg gccaggccga cgagatggag agatacgtgg aggagaacca     5760

gacccgggat aagcacctca accccaacga gtggtggaag gtgtacccta gcagcgtgac     5820

cgagttcaag ttcctgttcg tgagcggcca cttcaagggc aactacaagg cccagctgac     5880

caggctgaac cacatcacca actgcaatgg cgccgtgctg agcgtggagg agctgctgat     5940

cggcggcgag atgatcaaag ccggcaccct gacactggag gaggtgcggc gcaagttcaa     6000

caacggcgag atcaacttca gatcttgata actcgagctg tgccttctag ttgccagcca     6060

tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc     6120

ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg     6180

gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct     6240

ggggatgcgg tgggctctat ggcggaccga gcggccgcag gaacccctag tgatggagtt     6300

ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg     6360

acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcagga     6420

agctgtaagc ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag     6480

gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat     6540

gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc     6600

atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa     6660

ctcatcaatg tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa     6720

ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta     6780

cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact     6840

ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt     6900

cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc     6960

tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt     7020

aattgactga atatcaacgc ttatttgcag cctgaatggc gaatg                     7065


<210>  35
<211>  420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZFN LEFT

<400>  35

Met Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp 
1               5                   10                  15      


Tyr Lys Asp Asp Asp Asp Lys Met Ala Pro Lys Lys Lys Arg Lys Val 
            20                  25                  30          


Gly Ile His Gly Val Pro Ala Ala Met Ala Glu Arg Pro Phe Gln Cys 
        35                  40                  45              


Arg Ile Cys Met Arg Asn Phe Ser Gln Ser Ser Asp Leu Ser Arg His 
    50                  55                  60                  


Ile Arg Thr His Thr Gly Glu Lys Pro Phe Ala Cys Asp Ile Cys Gly 
65                  70                  75                  80  


Arg Lys Phe Ala Trp Arg Ser Ser Leu Arg Gln His Thr Lys Ile His 
                85                  90                  95      


Thr His Pro Arg Ala Pro Ile Pro Lys Pro Phe Gln Cys Arg Ile Cys 
            100                 105                 110         


Met Arg Asn Phe Ser Gln Ser Gly Asp Leu Thr Arg His Ile Arg Thr 
        115                 120                 125             


His Thr Gly Glu Lys Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys Phe 
    130                 135                 140                 


Ala Arg Arg Ala Asp Arg Ala Lys His Thr Lys Ile His Thr His Pro 
145                 150                 155                 160 


Arg Ala Pro Ile Pro Lys Pro Phe Gln Cys Arg Ile Cys Met Arg Asn 
                165                 170                 175     


Phe Ser Arg Ser Asp Asp Leu Thr Arg His Ile Arg Thr His Thr Gly 
            180                 185                 190         


Glu Lys Pro Phe Ala Cys Asp Ile Cys Gly Arg Lys Phe Ala Gln Arg 
        195                 200                 205             


Ser Thr Leu Ser Ser His Thr Lys Ile His Leu Arg Gly Ser Gln Leu 
    210                 215                 220                 


Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His Lys Leu 
225                 230                 235                 240 


Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala Arg Asn 
                245                 250                 255     


Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe Phe Met 
            260                 265                 270         


Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg Lys Pro 
        275                 280                 285             


Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly Val Ile 
    290                 295                 300                 


Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile Gly Gln 
305                 310                 315                 320 


Ala Asp Glu Met Glu Arg Tyr Val Glu Glu Asn Gln Thr Arg Asp Lys 
                325                 330                 335     


His Leu Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser Val Thr 
            340                 345                 350         


Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn Tyr Lys 
        355                 360                 365             


Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn Gly Ala Val 
    370                 375                 380                 


Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys Ala Gly 
385                 390                 395                 400 


Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly Glu Ile 
                405                 410                 415     


Asn Phe Arg Ser 
            420 


<210>  36
<211>  381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZFN RIGHT

<400>  36

Met Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp 
1               5                   10                  15      


Tyr Lys Asp Asp Asp Asp Lys Met Ala Pro Lys Lys Lys Arg Lys Val 
            20                  25                  30          


Gly Ile His Gly Val Pro Ala Ala Met Ala Glu Arg Pro Phe Gln Cys 
        35                  40                  45              


Arg Ile Cys Met Arg Asn Phe Ser Asp Gln Ser Asn Leu Arg Ala His 
    50                  55                  60                  


Ile Arg Thr His Thr Gly Glu Lys Pro Phe Ala Cys Asp Ile Cys Gly 
65                  70                  75                  80  


Arg Lys Phe Ala Arg Lys Ser Asp Arg Ile Lys His Thr Lys Ile His 
                85                  90                  95      


Thr Gly Ser Gln Lys Pro Phe Gln Cys Arg Ile Cys Met Arg Lys Phe 
            100                 105                 110         


Ala Arg Ser Asp Asn Leu Ser Val His Thr Lys Ile His Thr Gly Glu 
        115                 120                 125             


Lys Pro Phe Gln Cys Arg Ile Cys Met Arg Asn Phe Ser Glu Arg Gly 
    130                 135                 140                 


Thr Leu Ala Arg His Ile Arg Thr His Thr Gly Glu Lys Pro Phe Ala 
145                 150                 155                 160 


Cys Asp Ile Cys Gly Arg Lys Phe Ala Arg Ser Asp Ala Leu Thr Gln 
                165                 170                 175     


His Thr Lys Ile His Leu Arg Gly Ser Gln Leu Val Lys Ser Glu Leu 
            180                 185                 190         


Glu Glu Lys Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His 
        195                 200                 205             


Glu Tyr Ile Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg 
    210                 215                 220                 


Ile Leu Glu Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr 
225                 230                 235                 240 


Arg Gly Lys His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr 
                245                 250                 255     


Thr Val Gly Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala 
            260                 265                 270         


Tyr Ser Gly Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln 
        275                 280                 285             


Arg Tyr Val Lys Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn 
    290                 295                 300                 


Glu Trp Trp Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu 
305                 310                 315                 320 


Phe Val Ser Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg 
                325                 330                 335     


Leu Asn Arg Lys Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu 
            340                 345                 350         


Leu Leu Ile Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu 
        355                 360                 365             


Glu Val Arg Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe 
    370                 375                 380     


