                         SEQUENCE LISTING

<110>  Yeda Research and Development Co. Ltd.
       BAR-ZIV, Roy
       KARZBRUN, Eyal
       TAYAR, Alexandra
 
<120>  MICROFLUIDIC DEVICE FOR ANALYZING GENE EXPRESSION

<130>  60470

<150>  US 61/887,457
<151>  2013-10-07

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak consensus sequence

<400>  1
cccgccgccr ccatgg                                                       16


<210>  2
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Shine-Delgarno sequence

<400>  2
aggagg                                                                   6


<210>  3
<211>  238
<212>  PRT
<213>  Aequorea victoria

<400>  3

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Ile Val Pro Val Leu Ile 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val His Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Asp Tyr Gly Lys Leu Glu Ile Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Gly Tyr Gly Ile Gln Cys Phe Ala Arg Tyr Pro Glu His Met Lys Met 
65                  70                  75                  80  


Asn Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Gln Asp Asp Gly Lys Tyr Lys Thr Arg Gly Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Met 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Phe Asn Ser His Asn Val Tyr Ile Met Pro Asp Lys Ala Asn Asn Gly 
145                 150                 155                 160 


Leu Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Gly Gly Gly Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Thr Asn Val Pro Leu Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Ile Pro Ile Asn His Tyr Leu Ser Leu Gln Thr Ala Ile Ser 
        195                 200                 205             


Lys Asp Arg Asn Glu Thr Arg Asp His Met Val Phe Leu Glu Phe Phe 
    210                 215                 220                 


Ser Ala Cys Gly His Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  4
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cloning vector pSB3616

<400>  4

Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      


Pro Val Thr Lys Ala Arg Thr Pro Glu Met Pro Leu Gln Gly Thr Ala 
            20                  25                  30          


Val Asp Gly Gly Gly Gly Ser Met His Ala Ser Leu Glu Val Leu Glu 
        35                  40                  45              


Asn Arg Ala Ala Gln Gly Asp Ile Thr Ala Pro Gly Gly Ala Arg Arg 
    50                  55                  60                  


Leu Thr Gly Asp Gln Thr Ala Ala Leu Arg Asp Ser Leu Ser Asp Lys 
65                  70                  75                  80  


Pro Ala Lys Asn Ile Ile Leu Leu Ile Gly Asp Gly Met Gly Asp Ser 
                85                  90                  95      


Glu Ile Thr Ala Ala Arg Asn Tyr Ala Glu Gly Ala Gly Gly Phe Phe 
            100                 105                 110         


Lys Gly Ile Asp Ala Leu Pro Leu Thr Gly Gln Tyr Thr His Tyr Ala 
        115                 120                 125             


Leu Asn Lys Lys Thr Gly Lys Pro Asp Tyr Val Thr Asp Ser Ala Ala 
    130                 135                 140                 


Ser Ala Thr Ala Trp Ser Thr Gly Val Lys Thr Tyr Asn Gly Ala Leu 
145                 150                 155                 160 


Gly Val Asp Ile His Glu Lys Asp His Pro Thr Ile Leu Glu Met Ala 
                165                 170                 175     


Lys Ala Ala Gly Leu Ala Thr Gly Asn Val Ser Thr Ala Glu Leu Gln 
            180                 185                 190         


Asp Ala Thr Pro Ala Ala Leu Val Ala His Val Thr Ser Arg Lys Cys 
        195                 200                 205             


Tyr Gly Pro Ser Ala Thr Ser Glu Lys Cys Pro Gly Asn Ala Leu Glu 
    210                 215                 220                 


Lys Gly Gly Lys Gly Ser Ile Thr Glu Gln Leu Leu Asn Ala Arg Ala 
225                 230                 235                 240 


Asp Val Thr Leu Gly Gly Gly Ala Lys Thr Phe Ala Glu Thr Ala Thr 
                245                 250                 255     


Ala Gly Glu Trp Gln Gly Lys Thr Leu Arg Glu Gln Ala Gln Ala Arg 
            260                 265                 270         


Gly Tyr Gln Leu Val Ser Asp Ala Ala Ser Leu Asn Ser Val Thr Glu 
        275                 280                 285             


Ala Asn Gln Gln Lys Pro Leu Leu Gly Leu Phe Ala Asp Gly Asn Met 
    290                 295                 300                 


Pro Val Arg Trp Leu Gly Pro Lys Ala Thr Tyr His Gly Asn Ile Asp 
305                 310                 315                 320 


Lys Pro Ala Val Thr Cys Thr Pro Asn Pro Gln Arg Asn Asp Ser Val 
                325                 330                 335     


Pro Thr Leu Ala Gln Met Thr Asp Lys Ala Ile Glu Leu Leu Ser Lys 
            340                 345                 350         


Asn Glu Lys Gly Phe Phe Leu Gln Val Glu Gly Ala Ser Ile Asp Lys 
        355                 360                 365             


Gln Asp His Ala Ala Asn Pro Cys Gly Gln Ile Gly Glu Thr Val Asp 
    370                 375                 380                 


Leu Asp Glu Ala Val Gln Arg Ala Leu Glu Phe Ala Lys Lys Glu Gly 
385                 390                 395                 400 


Asn Thr Leu Val Ile Val Thr Ala Asp His Ala His Ala Ser Gln Ile 
                405                 410                 415     


Val Ala Pro Asp Thr Lys Ala Pro Gly Leu Thr Gln Ala Leu Asn Thr 
            420                 425                 430         


Lys Asp Gly Ala Val Met Val Met Ser Tyr Gly Asn Ser Glu Glu Asp 
        435                 440                 445             


Ser Gln Glu His Thr Gly Ser Gln Leu Arg Ile Ala Ala Tyr Gly Pro 
    450                 455                 460                 


His Ala Ala Asn Val Val Gly Leu Thr Asp Gln Thr Asp Leu Phe Tyr 
465                 470                 475                 480 


Thr Met Lys Ala Ala Leu Gly Leu Lys 
                485                 


<210>  5
<211>  309
<212>  PRT
<213>  Armoracia rusticana

<400>  5

Met Gln Leu Thr Pro Thr Phe Tyr Asp Asn Ser Cys Pro Asn Val Ser 
1               5                   10                  15      


Asn Ile Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg 
            20                  25                  30          


Ile Ala Ala Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn 
        35                  40                  45              


Gly Cys Asp Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr 
    50                  55                  60                  


Glu Lys Asp Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val 
65                  70                  75                  80  


Ile Asp Arg Met Lys Ala Ala Val Glu Ser Ala Cys Pro Arg Thr Val 
                85                  90                  95      


Ser Cys Ala Asp Leu Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu 
            100                 105                 110         


Ala Gly Gly Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu 
        115                 120                 125             


Gln Ala Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe 
    130                 135                 140                 


Thr Leu Pro Gln Leu Lys Asp Ser Phe Arg Asn Val Gly Leu Asn Arg 
145                 150                 155                 160 


Ser Ser Asp Leu Val Ala Leu Ser Gly Gly His Thr Phe Gly Lys Asn 
                165                 170                 175     


Gln Cys Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly 
            180                 185                 190         


Leu Pro Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly 
        195                 200                 205             


Leu Cys Pro Leu Asn Gly Asn Leu Ser Ala Leu Val Asp Phe Asp Leu 
    210                 215                 220                 


Arg Thr Pro Thr Ile Phe Asp Asn Lys Tyr Tyr Val Asn Leu Glu Glu 
225                 230                 235                 240 


Gln Lys Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asn 
                245                 250                 255     


Ala Thr Asp Thr Ile Pro Leu Val Arg Ser Phe Ala Asn Ser Thr Gln 
            260                 265                 270         


Thr Phe Phe Asn Ala Phe Val Glu Ala Met Asp Arg Met Gly Asn Ile 
        275                 280                 285             


Thr Pro Leu Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val 
    290                 295                 300                 


Val Asn Ser Asn Ser 
305                 


<210>  6
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (264)..(269)
<223>  Histidine tag

<400>  6

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Gly Ser Pro Gly Gln 
1               5                   10                  15      


Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Ser Asp Ile Gly Thr Tyr 
            20                  25                  30          


Lys Ile Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu 
        35                  40                  45              


Met Ile Tyr Asp Val Asn Gln Arg Pro Ser Gly Val Ser Asp Arg Phe 
    50                  55                  60                  


Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Tyr Thr Ser Gly 
                85                  90                  95      


Ser Thr Leu Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ser 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Ser Ala Leu 
        115                 120                 125             


Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
    130                 135                 140                 


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
145                 150                 155                 160 


Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ile Ile Tyr Pro Gly Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe 
            180                 185                 190         


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
        195                 200                 205             


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
    210                 215                 220                 


Ala Arg His Arg Ala Ala Ser Gly Ser Pro Asp Ala Cys Asp Tyr Trp 
225                 230                 235                 240 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Ser Ala Ser Ala Pro 
                245                 250                 255     


Thr Leu Phe Pro Ala Ala Ala His His His His His His Gly Ala Ala 
            260                 265                 270         


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Gly Ala Ala 
        275                 280                 285     


<210>  7
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (273)..(283)
<223>  Myc tag

<400>  7

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Gly Ser Pro Gly Gln 
1               5                   10                  15      


Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Ser Asp Ile Gly Thr Tyr 
            20                  25                  30          


Lys Ile Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu 
        35                  40                  45              


Met Ile Tyr Asp Val Asn Gln Arg Pro Ser Gly Val Ser Asp Arg Phe 
    50                  55                  60                  


Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Tyr Thr Ser Gly 
                85                  90                  95      


Ser Thr Leu Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ser 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Ser Ala Leu 
        115                 120                 125             


Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
    130                 135                 140                 


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
145                 150                 155                 160 


Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ile Ile Tyr Pro Gly Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe 
            180                 185                 190         


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
        195                 200                 205             


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
    210                 215                 220                 


Ala Arg His Arg Ala Ala Ser Gly Ser Pro Asp Ala Cys Asp Tyr Trp 
225                 230                 235                 240 


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Ser Ala Ser Ala Pro 
                245                 250                 255     


Thr Leu Phe Pro Ala Ala Ala His His His His His His Gly Ala Ala 
            260                 265                 270         


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Gly Ala Ala 
        275                 280                 285     


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin lygase tag

<400>  8

Leu His His Ile Leu Asp Ala Gln Lys Met Val Trp Asn His Arg 
1               5                   10                  15  


<210>  9
<211>  238
<212>  PRT
<213>  Aequorea macrodactyla

<400>  9

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val His Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Asp Tyr Gly Lys Leu Glu Ile Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Gly Tyr Gly Ile Leu Cys Phe Ala Arg Tyr Pro Glu His Met Lys Met 
65                  70                  75                  80  


Asn Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Gln Asp Asp Gly Lys Tyr Lys Thr Arg Gly Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Met 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Phe Asn Ser His Asn Val Tyr Ile Met Pro Asp Lys Ala Asn Asn Gly 
145                 150                 155                 160 


Leu Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Gly Gly Gly Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Thr Asn Val Pro Leu Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Ile Pro Ile Asn His Tyr Leu Ser Tyr Gln Thr Ala Ile Ser 
        195                 200                 205             


Lys Asp Arg Asn Glu Thr Arg Asp His Met Val Phe Leu Glu Phe Phe 
    210                 215                 220                 


Ser Ala Cys Gly His Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  10
<211>  1019
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Vector pFRT1-lacZ-Tel

<400>  10

Met Ala Asp Pro Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly 
1               5                   10                  15      


Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp 
            20                  25                  30          


Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg 
        35                  40                  45              


Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp Phe Pro Ala Pro Glu Ala 
    50                  55                  60                  


Val Pro Glu Ser Trp Leu Glu Cys Asp Leu Pro Glu Ala Asp Thr Val 
65                  70                  75                  80  


Val Val Pro Ser Asn Trp Gln Met His Gly Tyr Asp Ala Pro Ile Tyr 
                85                  90                  95      


Thr Asn Val Thr Tyr Pro Ile Thr Val Asn Pro Pro Phe Val Pro Thr 
            100                 105                 110         


Glu Asn Pro Thr Gly Cys Tyr Ser Leu Thr Phe Asn Val Asp Glu Ser 
        115                 120                 125             


Trp Leu Gln Glu Gly Gln Thr Arg Ile Ile Phe Asp Gly Val Asn Ser 
    130                 135                 140                 


Ala Phe His Leu Trp Cys Asn Gly Arg Trp Val Gly Tyr Gly Gln Asp 
145                 150                 155                 160 


Ser Arg Leu Pro Ser Glu Phe Asp Leu Ser Ala Phe Leu Arg Ala Gly 
                165                 170                 175     


Glu Asn Arg Leu Ala Val Met Val Leu Arg Trp Ser Asp Gly Ser Tyr 
            180                 185                 190         


Leu Glu Asp Gln Asp Met Trp Arg Met Ser Gly Ile Phe Arg Asp Val 
        195                 200                 205             


Ser Leu Leu His Lys Pro Thr Thr Gln Ile Ser Asp Phe His Val Ala 
    210                 215                 220                 


Thr Arg Phe Asn Asp Asp Phe Ser Arg Ala Val Leu Glu Ala Glu Val 
225                 230                 235                 240 


Gln Met Cys Gly Glu Leu Arg Asp Tyr Leu Arg Val Thr Val Ser Leu 
                245                 250                 255     


Trp Gln Gly Glu Thr Gln Val Ala Ser Gly Thr Ala Pro Phe Gly Gly 
            260                 265                 270         


Glu Ile Ile Asp Glu Arg Gly Gly Tyr Ala Asp Arg Val Thr Leu Arg 
        275                 280                 285             


Leu Asn Val Glu Asn Pro Lys Leu Trp Ser Ala Glu Ile Pro Asn Leu 
    290                 295                 300                 


Tyr Arg Ala Val Val Glu Leu His Thr Ala Asp Gly Thr Leu Ile Glu 
305                 310                 315                 320 


Ala Glu Ala Cys Asp Val Gly Phe Arg Glu Val Arg Ile Glu Asn Gly 
                325                 330                 335     


Leu Leu Leu Leu Asn Gly Lys Pro Leu Leu Ile Arg Gly Val Asn Arg 
            340                 345                 350         


His Glu His His Pro Leu His Gly Gln Val Met Asp Glu Gln Thr Met 
        355                 360                 365             


Val Gln Asp Ile Leu Leu Met Lys Gln Asn Asn Phe Asn Ala Val Arg 
    370                 375                 380                 


Cys Ser His Tyr Pro Asn His Pro Leu Trp Tyr Thr Leu Cys Asp Arg 
385                 390                 395                 400 


Tyr Gly Leu Tyr Val Val Asp Glu Ala Asn Ile Glu Thr His Gly Met 
                405                 410                 415     


Val Pro Met Asn Arg Leu Thr Asp Asp Pro Arg Trp Leu Pro Ala Met 
            420                 425                 430         


Ser Glu Arg Val Thr Arg Met Val Gln Arg Asp Arg Asn His Pro Ser 
        435                 440                 445             


Val Ile Ile Trp Ser Leu Gly Asn Glu Ser Gly His Gly Ala Asn His 
    450                 455                 460                 


Asp Ala Leu Tyr Arg Trp Ile Lys Ser Val Asp Pro Ser Arg Pro Val 
465                 470                 475                 480 


Gln Tyr Glu Gly Gly Gly Ala Asp Thr Thr Ala Thr Asp Ile Ile Cys 
                485                 490                 495     


Pro Met Tyr Ala Arg Val Asp Glu Asp Gln Pro Phe Pro Ala Val Pro 
            500                 505                 510         


Lys Trp Ser Ile Lys Lys Trp Leu Ser Leu Pro Gly Glu Thr Arg Pro 
        515                 520                 525             


Leu Ile Leu Cys Glu Tyr Ala His Ala Met Gly Asn Ser Leu Gly Gly 
    530                 535                 540                 


Phe Ala Lys Tyr Trp Gln Ala Phe Arg Gln Tyr Pro Arg Leu Gln Gly 
545                 550                 555                 560 


Gly Phe Val Trp Asp Trp Val Asp Gln Ser Leu Ile Lys Tyr Asp Glu 
                565                 570                 575     


Asn Gly Asn Pro Trp Ser Ala Tyr Gly Gly Asp Phe Gly Asp Thr Pro 
            580                 585                 590         


Asn Asp Arg Gln Phe Cys Met Asn Gly Leu Val Phe Ala Asp Arg Thr 
        595                 600                 605             


Pro His Pro Ala Leu Thr Glu Ala Lys His Gln Gln Gln Phe Phe Gln 
    610                 615                 620                 


Phe Arg Leu Ser Gly Gln Thr Ile Glu Val Thr Ser Glu Tyr Leu Phe 
625                 630                 635                 640 


Arg His Ser Asp Asn Glu Leu Leu His Trp Met Val Ala Leu Asp Gly 
                645                 650                 655     


Lys Pro Leu Ala Ser Gly Glu Val Pro Leu Asp Val Ala Pro Gln Gly 
            660                 665                 670         


Lys Gln Leu Ile Glu Leu Pro Glu Leu Pro Gln Pro Glu Ser Ala Gly 
        675                 680                 685             


Gln Leu Trp Leu Thr Val Arg Val Val Gln Pro Asn Ala Thr Ala Trp 
    690                 695                 700                 


Ser Glu Ala Gly His Ile Ser Ala Trp Gln Gln Trp Arg Leu Ala Glu 
705                 710                 715                 720 


Asn Leu Ser Val Thr Leu Pro Ala Ala Ser His Ala Ile Pro His Leu 
                725                 730                 735     


Thr Thr Ser Glu Met Asp Phe Cys Ile Glu Leu Gly Asn Lys Arg Trp 
            740                 745                 750         


Gln Phe Asn Arg Gln Ser Gly Phe Leu Ser Gln Met Trp Ile Gly Asp 
        755                 760                 765             


Lys Lys Gln Leu Leu Thr Pro Leu Arg Asp Gln Phe Thr Arg Ala Pro 
    770                 775                 780                 


Leu Asp Asn Asp Ile Gly Val Ser Glu Ala Thr Arg Ile Asp Pro Asn 
785                 790                 795                 800 


Ala Trp Val Glu Arg Trp Lys Ala Ala Gly His Tyr Gln Ala Glu Ala 
                805                 810                 815     


Ala Leu Leu Gln Cys Thr Ala Asp Thr Leu Ala Asp Ala Val Leu Ile 
            820                 825                 830         


Thr Thr Ala His Ala Trp Gln His Gln Gly Lys Thr Leu Phe Ile Ser 
        835                 840                 845             


Arg Lys Thr Tyr Arg Ile Asp Gly Ser Gly Gln Met Ala Ile Thr Val 
    850                 855                 860                 


Asp Val Glu Val Ala Ser Asp Thr Pro His Pro Ala Arg Ile Gly Leu 
865                 870                 875                 880 


Asn Cys Gln Leu Ala Gln Val Ala Glu Arg Val Asn Trp Leu Gly Leu 
                885                 890                 895     


Gly Pro Gln Glu Asn Tyr Pro Asp Arg Leu Thr Ala Ala Cys Phe Asp 
            900                 905                 910         


Arg Trp Asp Leu Pro Leu Ser Asp Met Tyr Thr Pro Tyr Val Phe Pro 
        915                 920                 925             


Ser Glu Asn Gly Leu Arg Cys Gly Thr Arg Glu Leu Asn Tyr Gly Pro 
    930                 935                 940                 


His Gln Trp Arg Gly Asp Phe Gln Phe Asn Ile Ser Arg Tyr Ser Gln 
945                 950                 955                 960 


Gln Gln Leu Met Glu Thr Ser His Arg His Leu Leu His Ala Glu Glu 
                965                 970                 975     


Gly Thr Trp Leu Asn Ile Asp Gly Phe His Met Gly Ile Gly Gly Asp 
            980                 985                 990         


Asp Ser Trp Ser Pro Ser Val Ser  Ala Asp Phe Gln Leu  Ser Ala Gly 
        995                 1000                 1005             


Arg Tyr  His Tyr Gln Leu Val  Trp Cys Gln Lys 
    1010                 1015                 


<210>  11
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct, streptavidin

<400>  11

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  12
<211>  717
<212>  DNA
<213>  Aequorea macrodactyla

<400>  12
atgagtaaag gagaagaact tttcactggg attgtcccag ttctcattga gttagacggt       60

gatgtccatg gacataaatt ctctgtcaga ggagaagggg aaggcgatgc agattatgga      120

aaacttgaaa tcaaattcat ttgcactact ggaaagctac cagttccatg gccaacactt      180

gttactacac tgggctacgg catccaatgt ttcgcaagat acccagaaca catgaaaatg      240

aatgacttct tcaagagtgc catgcctgag ggttacattc aagaaagaac catctttttc      300

caagatgatg gaaaatacaa gacacgtggt gaagtcaagt ttgaaggtga tactcttgtt      360

aacagaattg agctcaaagg tatggacttt aaagaagatg gcaatatcct tggacacaag      420

ttggagtaca attttaattc acataatgta tacattatgc cggacaaagc caataatgga      480

ctcaaagtca atttcaaaat tagacacaat atcgaaggtg gtggtgtcca acttgctgat      540

cattaccaaa caaatgttcc ccttggagac ggtcctgtcc ttataccaat caatcactac      600

ctatccttgc aaacagccat ttcaaaagat cgaaatgaga cgagagatca tatggtgttt      660

ctggaatttt tctcagcttg tggacataca catggcatgg atgaactata caaataa         717


<210>  13
<211>  7151
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning vector pSB3616 alkaline phosphatase (phoA), lac 
       repressor
       (lacI), and chloramphenicol acetyltransferase (cat) genes, 
       complete
 cds.

<400>  13
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     1440

atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc     1500

atgtttgaca gcttatcatc gataagcttt tttcgccagg cgcagacttg ctgttcttca     1560

ggcaatcact catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct     1620

gattacagga ggtcatacgc tgttgttgtg gggttaaagt tctctcggca gcgccgacgg     1680

tggttaaaaa aaccagaccg aaaagcaagg taaccagtaa tgttattttc atagcaccat     1740

ccctcttcat gttttaacca tgagcgtatg cgcccgtgat ctgccattaa gtctggttgc     1800

taacagcaaa aaaaccaccc ggcagcgaaa attcactgcc gggcgcggtt ttatttcagc     1860

cccagagcgg ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacaacattg     1920

gcggcatgcg ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc     1980

tcttcggagt tcccgtaact catcaccatc actgcgccat ctttggtatt tagcgcctgg     2040

gtgaggcccg gagctttggt atccggcgca acaatctggc tggcgtgggc gtgatcagcg     2100

gtgactatga ccagcgtgtt accctccttt ttagcgaatt ccagcgcccg ttgtacggct     2160

tcatcgagat cgaccgtctc gccaatttgc ccacaaggat tcgcagcatg atcctgttta     2220

tcgattgacg caccttcaac ttgcaggaaa aagcctttct catttttact caacaattca     2280

atggctttgt cggtcatctg cgccagggtt ggtacactgt cattacgttg cggatttggc     2340

gtacaggtga ctgcgggctt atcgatattg ccatggtacg ttgctttcgg tcctagccag     2400

cgcactggca tattgccgtc agcaaacagg ccaagcaggg gtttttgctg attcgcttcc     2460

gtcaccgaat tcagtgaggc agcatcgctc accaactgat aaccacgcgc ctgtgcctgt     2520

tcacgcagcg tttttccctg ccattcacca gcggttgccg tttcagcaaa ggtttttgcg     2580

ccgccgccaa gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat cgatcctttt     2640

ccgccttttt ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat     2700

ttgcgcgagg tcacatgtgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta     2760

gaaacgttac cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct     2820

ttttcgtgaa tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg     2880

gttgctgatg cagccgagtc ggtgacgtag tccggtttgc cggttttttt attcagcgca     2940

tagtgagtgt attgcccggt aagcggtaag gcatctatac ctttaaaaaa gccgcccgca     3000

ccttcggcat aattacgtgc ggcagtaatt tccgagtccc ccatcccatc gccaatcagc     3060

aaaataatat tttttgcagg tttatcgcta agagaatcac gcagagcggc agtctgatca     3120

cccgttaaac ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc ccggttttcc     3180

agaacctcga ggctagcatg catagaaccg ccaccaccgt cgacagcggt accctgcaga     3240

ggcatttctg gtgtccgggc ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt     3300

gcaatagtgc tttgtttcac tttattttct ccatgtcgcg tcttatcagg gggaattctg     3360

tttcctgtgt gaaattgtta tccgctcaca attccacaca ttatacgagc cgatgattaa     3420

ttgtcaacag ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca     3480

ttatctagag gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc     3540

gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca     3600

caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc     3660

acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg     3720

gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc     3780

tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc     3840

ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag     3900

agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc     3960

ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa     4020

acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca     4080

caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg     4140

cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc     4200

gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat     4260

cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc     4320

attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag     4380

acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat     4440

ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg     4500

gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata     4560

gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg     4620

aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca     4680

atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac     4740

gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt     4800

cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg     4860

aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat     4920

acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt     4980

tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta     5040

ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg     5100

ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt     5160

aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc     5220

gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt     5280

cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc     5340

ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc     5400

cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc     5460

caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag     5520

cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac     5580

atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt     5640

gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct     5700

aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg     5760

caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg     5820

cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg     5880

aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc     5940

gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg     6000

gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcccctac gtgctgctga     6060

agttgcccgc aacagagagt ggaaccaacc ggtgatacca cgatactatg actgagagtc     6120

aacgccatga gcggcctcat ttcttattct gagttacaac agtccgcacc gctgtccggt     6180

agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt     6240

atcatgcaac tcgtaggaca ggtgccggca gcgcccaaca gtcccccggc cacggggcct     6300

gccaccatac ccacgccgaa acaagcgccc tgcaccatta tgttccggat ctgcatcgca     6360

ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctaaccgttt     6420

ttatcaggct ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga     6480

gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag     6540

tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac     6600

cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat     6660

tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg     6720

ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca     6780

tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta     6840

taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa     6900

tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac     6960

cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt     7020

agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc     7080

tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc     7140

attgccatac g                                                          7151


<210>  14
<211>  955
<212>  DNA
<213>  Armoracia rusticana


<220>
<221>  misc_feature
<223>  A.rusticana synthetic gene for peroxidase

<400>  14
aagcttaacc atgcagttaa cccctacatt ctacgacaat agctgtccca acgtgtccaa       60

catcgttcgc gacacaatcg tcaacgagct cagatccgat cccaggatcg ctgcttcaat      120

attacgtctg cacttccatg actgcttcgt gaatggttgc gacgctagca tattactgga      180

caacaccacc agtttccgca ctgaaaagga tgcattcggg aacgctaaca gcgccagggg      240

ctttccagtg atcgatcgca tgaaggctgc cgttgagtca gcatgcccac gaacagtcag      300

ttgtgcagac ctgctgacta tagctgcgca acagagcgtg actcttgcag gcggaccgtc      360

ctggagagtg ccgctcggtc gacgtgactc cctacaggca ttcctagatc tggccaacgc      420

caacttgcct gctccattct tcaccctgcc ccagctgaag gatagcttta gaaacgtggg      480

tctgaatcgc tcgagtgacc ttgtggctct gtccggagga cacacatttg gaaagaacca      540

gtgtaggttc atcatggata ggctctacaa tttcagcaac actgggttac ctgaccccac      600

gctgaacact acgtatctcc agacactgag aggcttgtgc ccactgaatg gcaacctcag      660

tgcactagtg gactttgatc tgcggacccc aaccatcttc gataacaagt actatgtgaa      720

tctagaggag cagaaaggcc tgatacagag tgatcaagaa ctgtttagca gtccaaacgc      780

cactgacacc atcccactgg tgagaagttt tgctaactct actcaaacct tctttaacgc      840

cttcgtggaa gccatggacc gtatgggtaa cattacccct ctgacgggta cccaaggcca      900

gattcgtctg aactgcagag tggtcaacag caactcttaa taaggatccg aattc           955


<210>  15
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Synthetic construct SP-1A2 recombinant single chain Fv antibody
       mRNA, partial cds.


<220>
<221>  misc_feature
<222>  (790)..(807)
<223>  Histidine tag

<400>  15
cagtctgtgt tgacgcagcc gccctcagtg tctgggtctc ctggacagtc gatcaccatc       60

tcctgcactg gaaccagcag tgatattggg acttataaaa ttgtctcctg gtaccaacag      120

caccctggca aagcccccaa actcatgatt tatgacgtca atcagcggcc ctcaggggtt      180

tctgatcgct tctctggctc caagtctggc aacacggcct ccctgacaat ctctgggctc      240

caggctgagg acgaggctga ttattactgc agctcatata caagcggcag cactctggta      300

ttcggcgggg ggaccaagct gaccgtccta ggctcgagtg gtggaggcgg ttcaggcgga      360

ggtggctctg gcggtagtgc acttcaggta cagctgcagc agtcaggagc agaggtgaaa      420

aagcccgggg agtctctgaa gatctcctgt aagggttctg gatacagctt taccagctac      480

tggatcggct gggtgcgcca gatgcccggg aaaggcctgg agtggatggg gatcatctat      540

cctggtgact ctgataccag atacagcccg tccttccaag gccaggtcac catctcagcc      600

gacaagtcca tcagcaccgc ctacctgcag tggagcagcc tgaaggcctc ggacaccgcc      660

atgtattact gtgcgagaca tcgggccgct agtgggagcc cggacgcgtg tgactactgg      720

ggccagggaa ccctggtcac cgtctcctca gggagtgcat ccgccccaac ccttttcccc      780

gcggccgcac atcatcatca ccatcacggg gccgcagaac aaaaactcat ctcagaagag      840

gatctgaatg gggccgcata g                                                861


<210>  16
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct SP-1A2 recombinant single chain Fv antibody
       mRNA, partial cds.


<220>
<221>  misc_feature
<222>  (817)..(849)
<223>  Myc tag

<400>  16
cagtctgtgt tgacgcagcc gccctcagtg tctgggtctc ctggacagtc gatcaccatc       60

tcctgcactg gaaccagcag tgatattggg acttataaaa ttgtctcctg gtaccaacag      120

caccctggca aagcccccaa actcatgatt tatgacgtca atcagcggcc ctcaggggtt      180

tctgatcgct tctctggctc caagtctggc aacacggcct ccctgacaat ctctgggctc      240

caggctgagg acgaggctga ttattactgc agctcatata caagcggcag cactctggta      300

ttcggcgggg ggaccaagct gaccgtccta ggctcgagtg gtggaggcgg ttcaggcgga      360

ggtggctctg gcggtagtgc acttcaggta cagctgcagc agtcaggagc agaggtgaaa      420

aagcccgggg agtctctgaa gatctcctgt aagggttctg gatacagctt taccagctac      480

tggatcggct gggtgcgcca gatgcccggg aaaggcctgg agtggatggg gatcatctat      540

cctggtgact ctgataccag atacagcccg tccttccaag gccaggtcac catctcagcc      600

gacaagtcca tcagcaccgc ctacctgcag tggagcagcc tgaaggcctc ggacaccgcc      660

atgtattact gtgcgagaca tcgggccgct agtgggagcc cggacgcgtg tgactactgg      720

ggccagggaa ccctggtcac cgtctcctca gggagtgcat ccgccccaac ccttttcccc      780

gcggccgcac atcatcatca ccatcacggg gccgcagaac aaaaactcat ctcagaagag      840

gatctgaatg gggccgcata g                                                861


<210>  17
<211>  717
<212>  DNA
<213>  Aequorea macrodactyla

<400>  17
atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt       60

gatgtccatg gacataaatt ctctgtcaga ggagaagggg aaggcgatgc agattatgga      120

aaacttgaaa tcaaattcat ttgcactact ggaaagctac cagttccatg gccaacactt      180

gttactacac tgggctatgg catcctatgt ttcgcaagat acccagaaca catgaaaatg      240

aatgacttct tcaagagtgc catgcctgag ggttacattc aagaaagaac catctttttc      300

caagatgatg gaaaatacaa gacacgtggt gaagtcaagt ttgaaggtga tactcttgtt      360

aacagaattg agctcaaagg tatggacttt aaagaagatg gcaatatcct tggacacaag      420

ttggagtaca attttaactc acataatgta tacattatgc cggacaaagc caataatgga      480

ctcaaagtca atttcaaaat tagacacaat atcgaaggtg gtggtgtcca actcgctgat      540

cattaccaaa caaatgttcc ccttggagac ggtcctgtcc ttataccaat caatcactac      600

ctatcctatc aaacagccat ttcaaaagat cgaaatgaga cgagagatca tatggtgttt      660

ctggaatttt tctcagcttg tggacataca catggcatgg atgaactata caaataa         717


<210>  18
<211>  8388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Vector pFRT1-lacZ-Tel

<400>  18
acgttaaggg attttggtca tggacggcca gcaggtaggc cgacaggctc atgccggccg       60

ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg      120

ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc      180

cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat      240

aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg      300

gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat      360

atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt      420

atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa tatctaccga ctggaaacag      480

gcaaatgcag gaaattactg aactgagggg acaggcgaga gacgatgcca aagagctaca      540

ccgacgagct ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg      600

ctgcggttgc gttcctggcg gtgagggcgg atgtcgatat gcgtaaggag aaaataccgc      660

atcaggcgca tatttgaatg tatttagaaa aataaacaaa aagagtttgt agaaacgcaa      720

aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc      780

gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat      840

ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt      900

tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac      960

cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg     1020

accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat     1080

ttaatctgta tcaggctgaa aattaaggaa tcccccagga cccaacgctg cccgagtttg     1140

tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa     1200

ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac     1260

gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag     1320

tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc     1380

ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc     1440

agctggaaat ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg     1500

aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt     1560

tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg     1620

tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg     1680

tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg     1740

cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc     1800

gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca     1860

tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat     1920

aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt     1980

gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag     2040

cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc     2100

agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc     2160

cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg     2220

atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc     2280

gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct     2340

gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc     2400

tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga     2460

ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg     2520

ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg     2580

aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg     2640

gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg     2700

ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag     2760

tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg     2820

aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc     2880

tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg     2940

tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg     3000

ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg     3060

tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga     3120

ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga     3180

tcatcggtca gacgattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc     3240

acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa     3300

cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc     3360

tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc     3420

aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca     3480

tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga     3540

tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg     3600

acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg     3660

ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac     3720

tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga     3780

gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg     3840

aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc     3900

agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca     3960

gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac     4020

gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca     4080

acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg     4140

accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag     4200

tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc     4260

accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt     4320

tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt     4380

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg     4440

acgggatcag ccattttttt ctccttactt acttaggatc cccgggtacc gagctcgaat     4500

tggggatctt gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag     4560

agcgcttttg aagctaattc gagctcggta cccggggatc ccccgggctc gactgcatta     4620

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgctcgaat     4680

tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact     4740

ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt     4800

gttatgactg tttttttgta ctcgagcaga aagtcaaaag cctccgaccg gaggcttttg     4860

acttgagggg gatcgatccc ttatggctct gcacccggct ccatcaccaa caggtcgcgc     4920

acgcgcttca ctcggttgcg gatcgacact gccagcccaa caaagccggt tgccgccgcc     4980

gccaggatcg cgccgatgat gccggccaca ccggccatcg cccaccaggt cgccgccttc     5040

cggttccatt cctgctggta ctgcttcgca atgctggacc tcggctcacc ataggctgac     5100

cgctcgatgg cgtatgccgc ttctcccctt ggcgtaaaac ccagcgccgc aggcggcatt     5160

gccatgctgc ccgccgcttt cccgaccacg acgcgcgcac caggcttgcg gtccagacct     5220

tcggccacgg cgagctgcgc aaggacataa tcagccgccg acttggctcc acgcgcctcg     5280

atcagctctt gcactcgcgc gaaatccttg gcctccacgg ccgccatgaa tcgcgcacgc     5340

ggcgaaggct ccgcagggcc ggcgtcgtga tcgccgccga gaatgccctt caccaagttc     5400

gacgacacga aaatcatgct gacggctatc accatcatgc agacggatcg cacgaacccg     5460

cagaactcac ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag     5520

gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc     5580

aggccgccac ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc     5640

agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt     5700

actgccccga tcccggcaat ggcaaggact gccagcgccg cgatgaggaa gcgggtgccc     5760

cgcttcttca tcttcgcgcc tcgggcctcc aggccgccta cctgggcgaa aacatcggtg     5820

tttgtggcat tcatacggac tcctgttggg ccagctcgcg cacgggctgg cgggtcagct     5880

tggcttgaag atcgccacgc attgcggcga tctgcttctc ggcatccttg cgcttctgca     5940

cgccttcctg ctggatgcga ataacgtcct cgacggtctt gatgagcgtc gtctgaacct     6000

gcttgagcgt gtccacgtcg atcaccaggc gttggttctc cttcgccgtc tcgacggacg     6060

tgcgatgcag cagggccgca ttgcgcttca tcaggtcgtt ggtggtgtcg tcgatggccg     6120

tggccagttc gacggcgttc ttctgctcgt tgaggctcaa ggccagcatg aattgccgct     6180

tccacgccgg cacggtgatt tcgcggatgg tgtggaattt atcgaccagc atctggttgt     6240

tggcctggat catgcggatg gtcggcaggc tctgcatggc cgaatgttgc aaggcgatca     6300

ggtcgccgat gcgcttgtcc aggttggcaa ccatcgcatc gaggtcggcc agctcctgca     6360

cgcggccagg gtcgttcccg acattgccgc gcagaccctc ggcctgctcg cgcagctcgg     6420

caaggcggac cttgccggcc gcgatgtgga cgccaagaag gcggtgttcc tcgcgcacgg     6480

ctgcgaacat ttcgtcgagc gaggcattgc gctgcgcgat gccttgctgg gtggtctgca     6540

cttcgctgac caggtgttcg atctgctcgc gggtcgtgtc gaagcgcgcc atgaagcccg     6600

tcgaacggac gcggaagcgg tcgatcagcg ggccaatcag gggcaggcgg gaacggttgt     6660

cggacaaagg gccgacgttc agggaacggg ccttggcgac aacctgggtc agtttctcgc     6720

ctgcttcgtc caggtcgctg ttgcgcacct ggtccagcag gctatcggcg tagcgggacg     6780

tgtgctcggc cacgtcgcgg ccgaactcgg caacggtctg cggactgccg acctcgatcc     6840

gctgcgcgac cgcatggact tccggcacgt cgctttcctg caagcccagc tcgcgcaggg     6900

ttgccggggt catgtcgaag gcgacgatag gggccttggc gtcgtgcgtc gttttcagtg     6960

cgttcatagg gttctcccgc cgtgttattg gttgatgcct tccaggctct gcgaaaggct     7020

ccgcatgagc gcctggtgag ctttggccgc ctcggcgacc attgccggat tcatgttctt     7080

ggtggtgatg agcgcgaggg tgtgctgacg ccagacgggc accaggacgg atgccgtttc     7140

agagaagcgg tccagcatgt ccacggcctg cgcccgcgtg agcttcatct gagtgacgct     7200

catttcatgg gacgccatga gggttgccag gttggcgagc ttgcgcgcga agcgttcgcg     7260

cggcttgtcg aactcgatca cgccggcctt ggccgcgccg gcctcggggt tctcgtccag     7320

gaactcgcgc ccggcttgaa tgtaggctct gagccggtct acctcggcct catgcgtatt     7380

gagcatgtca tccaaggcgc gcaacgtgtc ccgcacgcgc tgcgctacgc cctcggcttc     7440

gtccagcaac tggtcgagcg tcttgcgggc gacctgatac ctcacctggc gttcaacctc     7500

acggccaagc atcttctcga accaggtagg cttttccgcg atcttgcggg ggtccgcgtc     7560

ggccagcttc gccacgatct ggctgatttt gtcggccagc gcggcaactg cgccgtgctc     7620

catcagattc gacagctcgt tgagggaatc cgccccgtcg atgccggccc cgtactcgcc     7680

aatcgtcgcc ggcgacgcga agagggcggg caaaacctcc cccttcaatc gcgccatgtt     7740

cacgctttgt tcttccatgg tatatctcct tcttaaagtt aaacaaaatt attcggaacc     7800

cagcatgata ttccggaaat accaactaag tcaacggctg atggccaatt cggcttcctc     7860

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     7920

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagatcg     7980

atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg tactaagctc     8040

tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg ctaacgtact     8100

aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc tcatgtttga     8160

acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa gttttataag     8220

aaaaaaaaga atatataagg cttttaaagc tagcttttaa ggtttaacgg ttgtggacaa     8280

caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa ttttcagtga     8340

cacaggaaca cttaacggct gacatgacgc tcagtggaac gaaaactc                  8388


<210>  19
<211>  483
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Synthetic construct streptavidin gene

<400>  19
gacccgagca aagattctaa agcacaagta tctgctgcag aagcaggaat tacaggcaca       60

tggtataatc agctgggatc tacatttatt gttacagccg gcgcagatgg agctcttaca      120

ggaacatatg aatctgctgt tggaaatgca gaatctagat acgtgcttac aggaagatat      180

gattctgcac ctgcaacaga tggatccgga acagcacttg gatggacagt tgcatggaaa      240

aacaattata gaaacgcaca tagcgctaca acatggtctg gccaatatgt gggaggtgca      300

gaagcaagaa ttaacacaca atggctttta acatctggaa caacagaagc aaatgcatgg      360

aaaagtactc ttgttggaca tgatacattt acaaaagtta aacctagcgc agcatctatc      420

gatgcagcga aaaaagcagg agttaacaat ggcaatcctt tagatgcagt tcaacaataa      480

tga                                                                    483


