                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  REVERSE TRANSCRIPTASE WITH ENHANCED PROPERTIES

<130>  NEB-371-PCT

<150>  61/891,554
<151>  2013-10-16

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Syntheitic construct

<400>  1

Met Pro Ile Ser Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly 
1               5                   10                  15      


Met Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile 
            20                  25                  30          


Lys Ala Leu Val Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile 
        35                  40                  45              


Ser Lys Ile Gly Pro Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile 
    50                  55                  60                  


Lys Lys Lys Asp Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu 
65                  70                  75                  80  


Leu Asn Lys Arg Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro 
                85                  90                  95      


His Pro Ala Gly Leu Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val 
            100                 105                 110         


Gly Asp Ala Tyr Phe Ser Val Pro Leu Asp Glu Asp Phe Arg Lys Tyr 
        115                 120                 125             


Thr Ala Phe Thr Ile Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg 
    130                 135                 140                 


Tyr Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile 
145                 150                 155                 160 


Phe Gln Ser Ser Met Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn 
                165                 170                 175     


Pro Asp Ile Val Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser 
            180                 185                 190         


Asp Leu Glu Ile Gly Gln His Arg Thr Lys Ile Glu Glu Leu Arg Gln 
        195                 200                 205             


His Leu Leu Arg Trp Gly Leu Thr Thr Pro Asp Lys Lys His Gln Lys 
    210                 215                 220                 


Glu Pro Pro Phe Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp 
225                 230                 235                 240 


Thr Val Gln Pro Ile Val Leu Pro Glu Lys Asp Ser Trp Thr Val Asn 
                245                 250                 255     


Asp Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr 
            260                 265                 270         


Pro Gly Ile Lys Val Arg Gln Leu Cys Lys Leu Leu Arg Gly Thr Lys 
        275                 280                 285             


Ala Leu Thr Glu Val Ile Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu 
    290                 295                 300                 


Ala Glu Asn Arg Glu Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr 
305                 310                 315                 320 


Asp Pro Ser Lys Asp Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly 
                325                 330                 335     


Gln Trp Thr Tyr Gln Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr 
            340                 345                 350         


Gly Lys Tyr Ala Arg Met Arg Gly Ala His Thr Asn Asp Val Lys Gln 
        355                 360                 365             


Leu Thr Glu Ala Val Gln Lys Ile Thr Thr Glu Ser Ile Val Ile Trp 
    370                 375                 380                 


Gly Lys Thr Pro Lys Phe Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu 
385                 390                 395                 400 


Thr Trp Trp Thr Glu Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu 
                405                 410                 415     


Phe Val Asn Thr Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys 
            420                 425                 430         


Glu Pro Ile Val Gly Ala Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn 
        435                 440                 445             


Arg Glu Thr Lys Leu Gly Lys Ala Gly Tyr Val Thr Asn Arg Gly Arg 
    450                 455                 460                 


Gln Lys Val Val Thr Leu Thr Asp Thr Thr Asn Gln Lys Thr Glu Leu 
465                 470                 475                 480 


Gln Ala Ile Tyr Leu Ala Leu Gln Asp Ser Gly Leu Glu Val Asn Ile 
                485                 490                 495     


Val Thr Asp Ser Gln Tyr Ala Leu Gly Ile Ile Gln Ala Gln Pro Asp 
            500                 505                 510         


Gln Ser Glu Ser Glu Leu Val Asn Gln Ile Ile Glu Gln Leu Ile Lys 
        515                 520                 525             


Lys Glu Lys Val Tyr Leu Ala Trp Val Pro Ala His Lys Gly Ile Gly 
    530                 535                 540                 


Gly Asn Glu Gln Val Asp Lys Leu Val Ser Ala Gly Ile Arg Lys Val 
545                 550                 555                 560 


Leu 
    


<210>  2
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2

Met Pro Ile Ser Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly 
1               5                   10                  15      


Met Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile 
            20                  25                  30          


Lys Ala Leu Thr Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile 
        35                  40                  45              


Ser Lys Ile Gly Pro Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile 
    50                  55                  60                  


Lys Lys Lys Asp Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu 
65                  70                  75                  80  


Leu Asn Lys Arg Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro 
                85                  90                  95      


His Pro Ala Gly Leu Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val 
            100                 105                 110         


Gly Asp Ala Tyr Phe Ser Val Pro Leu Asp Lys Asp Phe Arg Lys Tyr 
        115                 120                 125             


Thr Ala Phe Thr Ile Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg 
    130                 135                 140                 


Tyr Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile 
145                 150                 155                 160 


Phe Gln Ser Ser Met Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn 
                165                 170                 175     


Pro Glu Ile Val Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser 
            180                 185                 190         


Asp Leu Glu Ile Gly Gln His Arg Thr Lys Ile Glu Glu Leu Arg Glu 
        195                 200                 205             


His Leu Leu Lys Trp Gly Phe Thr Thr Pro Asp Lys Lys His Gln Lys 
    210                 215                 220                 


Glu Pro Pro Phe Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp 
225                 230                 235                 240 


Thr Val Gln Pro Ile Gln Leu Pro Glu Lys Asp Ser Trp Thr Val Asn 
                245                 250                 255     


Asp Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr 
            260                 265                 270         


Pro Gly Ile Lys Val Lys Gln Leu Cys Lys Leu Leu Arg Gly Ala Lys 
        275                 280                 285             


Ala Leu Thr Asp Val Val Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu 
    290                 295                 300                 


Ala Glu Asn Arg Glu Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr 
305                 310                 315                 320 


Asp Pro Ser Lys Asp Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly 
                325                 330                 335     


Gln Trp Thr Tyr Gln Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr 
            340                 345                 350         


Gly Lys Tyr Ala Lys Met Arg Ser Ala His Thr Asn Asp Val Lys Gln 
        355                 360                 365             


Leu Thr Glu Val Val Gln Lys Ile Ala Thr Glu Ser Ile Val Ile Trp 
    370                 375                 380                 


Gly Lys Thr Pro Lys Phe Arg Leu Pro Ile Gln Lys Glu Thr Trp Glu 
385                 390                 395                 400 


Thr Trp Trp Thr Glu Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu 
                405                 410                 415     


Phe Val Asn Thr Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Thr 
            420                 425                 430         


Glu Pro Ile Val Gly Ala Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn 
        435                 440                 445             


Arg Glu Thr Lys Leu Gly Lys Ala Gly Tyr Val Thr Asp Arg Gly Arg 
    450                 455                 460                 


Gln Lys Val Val Ser Leu Thr Glu Thr Thr Asn Gln Lys Thr Glu Leu 
465                 470                 475                 480 


Gln Ala Ile Gln Leu Ala Leu Gln Asp Ser Gly Ser Glu Val Asn Ile 
                485                 490                 495     


Val Thr Asp Ser Gln Tyr Ala Leu Gly Ile Ile Gln Ala Gln Pro Asp 
            500                 505                 510         


Lys Ser Glu Ser Glu Leu Val Asn Gln Ile Ile Glu Gln Leu Ile Lys 
        515                 520                 525             


Lys Glu Lys Val Tyr Leu Ser Trp Val Pro Ala His Lys Gly Ile Gly 
    530                 535                 540                 


Gly Asn Glu Gln Val Asp Lys Leu Val Ser Ser Gly Ile Arg Lys Val 
545                 550                 555                 560 


Leu 
    


<210>  3
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3

Phe Pro Ile Ser Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly 
1               5                   10                  15      


Met Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile 
            20                  25                  30          


Lys Ala Leu Val Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile 
        35                  40                  45              


Ser Lys Ile Gly Pro Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile 
    50                  55                  60                  


Lys Lys Lys Asp Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu 
65                  70                  75                  80  


Leu Asn Lys Arg Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro 
                85                  90                  95      


His Pro Ala Gly Leu Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val 
            100                 105                 110         


Gly Asp Ala Tyr Phe Ser Val Pro Leu Asp Lys Glu Phe Arg Lys Tyr 
        115                 120                 125             


Thr Ala Phe Thr Ile Pro Ser Ile Asn Asn Glu Thr Pro Arg Ile Arg 
    130                 135                 140                 


Tyr Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile 
145                 150                 155                 160 


Phe Gln Ser Ser Met Thr Lys Ile Leu Glu Pro Phe Lys Lys Gln Asn 
                165                 170                 175     


Pro Glu Ile Val Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser 
            180                 185                 190         


Asp Leu Glu Ile Gly Gln His Arg Ile Lys Ile Glu Glu Leu Arg Glu 
        195                 200                 205             


His Leu Leu Lys Trp Gly Phe Thr Thr Pro Asp Lys Lys His Gln Lys 
    210                 215                 220                 


Glu Pro Pro Phe Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp 
225                 230                 235                 240 


Thr Val Gln Pro Ile Val Leu Pro Glu Lys Asp Ser Trp Thr Val Asn 
                245                 250                 255     


Asp Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr 
            260                 265                 270         


Ala Gly Ile Lys Val Lys Gln Leu Cys Lys Leu Leu Arg Gly Thr Lys 
        275                 280                 285             


Ala Leu Thr Glu Val Val Gln Leu Thr Lys Glu Ala Glu Leu Glu Leu 
    290                 295                 300                 


Ala Glu Asn Arg Glu Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr 
305                 310                 315                 320 


Asp Pro Ser Lys Asp Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly 
                325                 330                 335     


Gln Trp Thr Tyr Gln Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr 
            340                 345                 350         


Gly Lys Tyr Ala Arg Met Arg Gly Ala His Thr Asn Asp Val Lys Gln 
        355                 360                 365             


Leu Thr Glu Ala Val Gln Lys Val Ala Thr Glu Ser Ile Val Ile Trp 
    370                 375                 380                 


Gly Lys Thr Pro Lys Phe Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu 
385                 390                 395                 400 


Ala Trp Trp Thr Glu Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu 
                405                 410                 415     


Phe Val Asn Thr Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys 
            420                 425                 430         


Glu Pro Ile Ile Gly Ala Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn 
        435                 440                 445             


Arg Glu Thr Lys Leu Gly Lys Ala Gly Tyr Val Thr Asp Arg Gly Arg 
    450                 455                 460                 


Gln Lys Val Val Ser Leu Thr Asp Thr Thr Asn Gln Lys Thr Glu Leu 
465                 470                 475                 480 


Gln Ala Ile His Leu Ala Leu Gln Asp Ser Gly Leu Glu Val Asn Ile 
                485                 490                 495     


Val Thr Asp Ser Gln Tyr Ala Leu Gly Ile Ile Gln Ala Gln Pro Asp 
            500                 505                 510         


Lys Ser Glu Ser Glu Leu Val Ser Gln Ile Ile Glu Gln Leu Ile Lys 
        515                 520                 525             


Lys Glu Lys Val Tyr Leu Ala Trp Val Pro Ala His Lys Gly Ile Gly 
    530                 535                 540                 


Gly Asn Glu Gln Val Asp Arg Leu Val Ser Thr Gly Ile Arg Lys Val 
545                 550                 555                 560 


Leu 
    


<210>  4
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4

Phe Pro Ile Ser Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly 
1               5                   10                  15      


Met Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Thr Thr Glu Lys Ile 
            20                  25                  30          


Glu Ala Leu Arg Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile 
        35                  40                  45              


Ser Arg Ile Gly Pro Glu Asn Pro Tyr Asn Thr Pro Ile Phe Ala Ile 
    50                  55                  60                  


Lys Lys Lys Asp Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu 
65                  70                  75                  80  


Leu Asn Lys Arg Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro 
                85                  90                  95      


His Pro Ala Gly Leu Lys Gln Lys Lys Ser Val Thr Val Leu Asp Val 
            100                 105                 110         


Gly Asp Ala Tyr Phe Ser Cys Pro Leu Asp Lys Asp Phe Arg Lys Tyr 
        115                 120                 125             


Thr Ala Phe Thr Ile Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg 
    130                 135                 140                 


Tyr Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile 
145                 150                 155                 160 


Phe Gln Ser Thr Met Thr Lys Ile Leu Glu Pro Phe Arg Glu Lys His 
                165                 170                 175     


Pro Glu Ile Ile Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser 
            180                 185                 190         


Asp Leu Glu Leu Ala Gln His Arg Glu Ala Val Glu Asp Leu Arg Asp 
        195                 200                 205             


His Leu Leu Lys Trp Gly Phe Thr Thr Pro Asp Lys Lys His Gln Lys 
    210                 215                 220                 


Glu Pro Pro Phe Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp 
225                 230                 235                 240 


Thr Val Gln Pro Ile Lys Leu Pro Glu Lys Asp Val Trp Thr Val Asn 
                245                 250                 255     


Asp Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr 
            260                 265                 270         


Pro Gly Ile Arg Val Lys Gln Leu Cys Lys Leu Ile Arg Gly Thr Lys 
        275                 280                 285             


Ala Leu Thr Glu Val Val Asn Phe Thr Glu Glu Ala Glu Leu Glu Leu 
    290                 295                 300                 


Ala Glu Asn Arg Glu Ile Leu Lys Glu Pro Leu His Gly Val Tyr Tyr 
305                 310                 315                 320 


Asp Pro Gly Lys Glu Leu Val Ala Glu Ile Gln Lys Gln Gly Gln Gly 
                325                 330                 335     


Gln Trp Thr Tyr Gln Ile Tyr Gln Glu Leu His Lys Asn Leu Lys Thr 
            340                 345                 350         


Gly Lys Tyr Ala Lys Met Arg Ser Ala His Thr Asn Asp Ile Lys Gln 
        355                 360                 365             


Leu Val Glu Val Val Arg Lys Val Ala Thr Glu Ser Ile Val Ile Trp 
    370                 375                 380                 


Gly Lys Thr Pro Lys Phe Arg Leu Pro Val Gln Lys Glu Val Trp Glu 
385                 390                 395                 400 


Ala Trp Trp Thr Asp His Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu 
                405                 410                 415     


Phe Val Asn Thr Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Thr 
            420                 425                 430         


Glu Pro Ile Ser Gly Ala Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn 
        435                 440                 445             


Arg Glu Thr Lys Leu Gly Lys Ala Gly Phe Val Thr Asp Arg Gly Arg 
    450                 455                 460                 


Gln Lys Val Val Ser Ile Ala Asp Thr Thr Asn Gln Lys Ala Glu Leu 
465                 470                 475                 480 


Gln Ala Ile Leu Met Ala Leu Gln Glu Ser Gly Arg Asp Val Asn Ile 
                485                 490                 495     


Val Thr Asp Ser Gln Tyr Ala Met Gly Ile Ile His Ser Gln Pro Asp 
            500                 505                 510         


Lys Ser Glu Ser Glu Leu Val Ser Gln Ile Ile Glu Glu Leu Ile Lys 
        515                 520                 525             


Lys Glu Arg Val Tyr Leu Ser Trp Val Pro Ala His Lys Gly Ile Gly 
    530                 535                 540                 


Gly Asn Glu Gln Val Asp Lys Leu Val Ser Ser Gly Ile Arg Lys Ile 
545                 550                 555                 560 


Leu 
    


<210>  5
<211>  560
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5

Phe Pro Ile Ser Pro Ile Glu Val Val Lys Val Gln Leu Lys Glu Gly 
1               5                   10                  15      


Met Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Ser Lys Glu Lys Ile 
            20                  25                  30          


Glu Ala Leu Thr Glu Ile Cys Lys Thr Leu Glu Lys Glu Gly Lys Ile 
        35                  40                  45              


Ser Ala Val Gly Pro Glu Asn Pro Tyr Asn Thr Pro Ile Phe Ala Ile 
    50                  55                  60                  


Lys Lys Lys Asp Thr Ser Lys Trp Arg Lys Leu Val Asp Phe Arg Glu 
65                  70                  75                  80  


Leu Asn Lys Arg Thr Gln Asp Phe Trp Glu Leu Gln Leu Gly Ile Pro 
                85                  90                  95      


His Pro Ala Gly Leu Arg Lys Arg Asn Met Val Thr Val Leu Asp Val 
            100                 105                 110         


Gly Asp Ala Tyr Phe Ser Ile Pro Leu Asp Pro Asp Phe Arg Lys Tyr 
        115                 120                 125             


Thr Ala Phe Thr Ile Pro Ser Leu Asn Asn Asn Thr Pro Gly Lys Arg 
    130                 135                 140                 


Phe Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile 
145                 150                 155                 160 


Phe Gln Ser Ser Met Thr Lys Ile Leu Asp Pro Phe Arg Lys Glu His 
                165                 170                 175     


Pro Asp Val Asp Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Ile Gly Ser 
            180                 185                 190         


Asp Leu Asn Glu Glu Glu His Arg Lys Leu Ile Lys Lys Leu Arg Gln 
        195                 200                 205             


His Leu Leu Thr Trp Gly Leu Glu Thr Pro Asp Lys Lys Tyr Gln Glu 
    210                 215                 220                 


Lys Pro Pro Phe Met Trp Met Gly Tyr Glu Leu His Pro Asn Lys Trp 
225                 230                 235                 240 


Thr Val Gln Asn Ile Thr Leu Pro Glu Pro Glu Gln Trp Thr Val Asn 
                245                 250                 255     


His Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr 
            260                 265                 270         


His Gly Ile Lys Thr Lys Glu Leu Cys Lys Leu Ile Arg Gly Val Lys 
        275                 280                 285             


Gly Leu Thr Glu Pro Val Glu Met Thr Arg Glu Ala Glu Leu Glu Leu 
    290                 295                 300                 


Glu Glu Asn Lys Gln Ile Leu Lys Glu Lys Val Gln Gly Ala Tyr Tyr 
305                 310                 315                 320 


Asp Pro Lys Leu Pro Leu Gln Ala Ala Ile Gln Lys Gln Gly Gln Gly 
                325                 330                 335     


Gln Trp Thr Tyr Gln Ile Tyr Gln Glu Glu Gly Lys Asn Leu Lys Thr 
            340                 345                 350         


Gly Lys Tyr Ala Lys Ser Pro Gly Thr His Thr Asn Glu Ile Arg Gln 
        355                 360                 365             


Leu Ala Gly Leu Ile Gln Lys Ile Gly Asn Glu Ser Ile Ile Ile Trp 
    370                 375                 380                 


Gly Ile Val Pro Lys Phe Leu Leu Pro Val Ser Lys Glu Thr Trp Ser 
385                 390                 395                 400 


Gln Trp Trp Thr Asp Tyr Trp Gln Val Thr Trp Val Pro Glu Trp Glu 
                405                 410                 415     


Phe Ile Asn Thr Pro Pro Leu Ile Arg Leu Trp Tyr Asn Leu Leu Ser 
            420                 425                 430         


Asp Pro Ile Pro Glu Ala Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn 
        435                 440                 445             


Arg Asp Ser Lys Lys Gly Arg Ala Gly Tyr Val Thr Asn Arg Gly Arg 
    450                 455                 460                 


Tyr Arg Ser Lys Asp Leu Glu Asn Thr Thr Asn Gln Gln Ala Glu Leu 
465                 470                 475                 480 


Trp Ala Val Asp Leu Ala Leu Lys Asp Ser Gly Ala Gln Val Asn Ile 
                485                 490                 495     


Val Thr Asp Ser Gln Tyr Val Met Gly Val Leu Gln Gly Leu Pro Asp 
            500                 505                 510         


Gln Ser Asp Ser Pro Ile Val Glu Gln Ile Ile Gln Lys Leu Thr Gln 
        515                 520                 525             


Lys Thr Ala Ile Tyr Leu Ala Trp Val Pro Ala His Lys Gly Ile Gly 
    530                 535                 540                 


Gly Asn Glu Glu Val Asp Lys Leu Val Ser Lys Asn Ile Arg Lys Ile 
545                 550                 555                 560 


<210>  6
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6

Pro Ile Ser Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly Met 
1               5                   10                  15      


Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile Lys 
            20                  25                  30          


Ala Leu Thr Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile Ser 
        35                  40                  45              


Lys Ile Gly Pro Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile Lys 
    50                  55                  60                  


Lys Lys Asp Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu Leu 
65                  70                  75                  80  


Asn Lys Arg Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro His 
                85                  90                  95      


Pro Ala Gly Leu Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val Gly 
            100                 105                 110         


Asp Ala Tyr Phe Ser Val Pro Leu Asp Lys Asp Phe Arg Lys Tyr Thr 
        115                 120                 125             


Ala Phe Thr Ile Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg Tyr 
    130                 135                 140                 


Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile Phe 
145                 150                 155                 160 


Gln Ser Ser Met Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn Pro 
                165                 170                 175     


Glu Ile Val Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser Asp 
            180                 185                 190         


Leu Glu Ile Gly Gln His Arg Thr Lys Ile Glu Glu Leu Arg Glu His 
        195                 200                 205             


Leu Leu Lys Trp Gly Phe Thr Thr Pro Asp Lys Lys His Gln Lys Glu 
    210                 215                 220                 


Pro Pro Phe Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp Thr 
225                 230                 235                 240 


Val Gln Pro Ile Gln Leu Pro Glu Lys Asp Ser Trp Thr Val Asn Asp 
                245                 250                 255     


Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr Pro 
            260                 265                 270         


Gly Ile Lys Val Lys Gln Leu Cys Lys Leu Leu Arg Gly Ala Lys Ala 
        275                 280                 285             


Leu Thr Asp Val Val Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu Ala 
    290                 295                 300                 


Glu Asn Arg Glu Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr Asp 
305                 310                 315                 320 


Pro Ser Lys Asp Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly Gln 
                325                 330                 335     


Trp Thr Tyr Gln Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr Gly 
            340                 345                 350         


Lys Tyr Ala Arg Met Arg Ser Ala His Thr Asn Asp Val Lys Gln Leu 
        355                 360                 365             


Thr Glu Val Val Gln Lys Ile Ala Thr Glu Ser Ile Val Ile Trp Gly 
    370                 375                 380                 


Lys Thr Pro Lys Phe Arg Leu Pro Ile Gln Lys Glu Thr Trp Glu Thr 
385                 390                 395                 400 


Trp Trp Thr Glu Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu Phe 
                405                 410                 415     


Val Asn Thr Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Thr Glu 
            420                 425                 430         


Pro Ile Val Gly Ala Glu Thr Phe 
        435                 440 


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
agtaccccat cgagcacg                                                     18


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
agcctggata gcaacgtaca                                                   20


<210>  9
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
gagccacacg cagctcattg tatcaccaac tgggacgaca                             40


<210>  10
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
ctgaacccca aggccaaccg gctggggtgt tgaaggtc                               38


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
tgtggtgcca gattttctcc a                                                 21


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
cgagaagatg acccagatca tgt                                               23


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
ctggaacggt gaaggtgaca                                                   20


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
aagggacttc ctgtaacaac gca                                               23


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
tgctgtctcc atgtttgatg tatct                                             25


<210>  16
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
tctctgctcc ccacctctaa gt                                                22


