                         SEQUENCE LISTING

<110>  Fred Hutchinson Cancer Research Center
       Seattle Children's Hospital, D/B/A Seattle Children's 
       Research Institute
 
<120>  MODIFIED HEMATOPOIETIC STEM/PROGENITOR AND NON-T EFFECTOR CELLS, 
       AND USES THEREOF

<130>  F053-0012PCT

<150>  61/898,387
<151>  2013-10-31

<160>  117   

<170>  PatentIn version 3.5

<210>  1
<211>  126
<212>  DNA
<213>  Homo sapiens

<400>  1
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactg                                                                 126


<210>  2
<211>  135
<212>  DNA
<213>  Homo sapiens

<400>  2
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactgcggg tgaag                                                       135


<210>  3
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile 
1               5                   10                  15      


Gly Leu Gly Ile Phe Phe 
            20          


<210>  4
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  4

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
        35                  40                  45  


<210>  5
<211>  210
<212>  DNA
<213>  Homo sapiens

<400>  5
atgttctggg tgctggtggt ggtgggcggg gtgctggcct gctacagcct gctggtgaca       60

gtggccttca tcatcttttg ggtgaaacgg ggcagaaaga aactcctgta tatattcaaa      120

caaccattta tgagaccagt acaaactact caagaggaag atggctgtag ctgccgattt      180

ccagaagaag aagaaggagg atgtgaactg                                       210


<210>  6
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  6

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  7
<211>  556
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met 
1               5                   10                  15      


Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp 
            20                  25                  30          


Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln 
        35                  40                  45              


Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu 
    50                  55                  60                  


Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ser 
65                  70                  75                  80  


Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu 
                85                  90                  95      


Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr 
            100                 105                 110         


Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp 
        115                 120                 125             


Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro 
    130                 135                 140                 


Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala 
145                 150                 155                 160 


Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Val Pro Pro 
                165                 170                 175     


Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro 
            180                 185                 190         


Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser 
        195                 200                 205             


Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser 
    210                 215                 220                 


Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp 
225                 230                 235                 240 


Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala 
                245                 250                 255     


Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu 
            260                 265                 270         


Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly 
        275                 280                 285             


Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu 
    290                 295                 300                 


Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg 
305                 310                 315                 320 


Arg Lys Arg Lys Arg Met Thr Asp Pro Thr Arg Arg Phe Phe Lys Val 
                325                 330                 335     


Thr Pro Pro Pro Gly Ser Gly Pro Gln Asn Gln Tyr Gly Asn Val Leu 
            340                 345                 350         


Ser Leu Pro Thr Pro Thr Ser Gly Leu Gly Arg Ala Gln Arg Trp Ala 
        355                 360                 365             


Ala Gly Leu Gly Gly Thr Ala Pro Ser Tyr Gly Asn Pro Ser Ser Asp 
    370                 375                 380                 


Val Gln Ala Asp Gly Ala Leu Gly Ser Arg Ser Pro Pro Gly Val Gly 
385                 390                 395                 400 


Pro Glu Glu Glu Glu Gly Glu Gly Tyr Glu Glu Pro Asp Ser Glu Glu 
                405                 410                 415     


Asp Ser Glu Phe Tyr Glu Asn Asp Ser Asn Leu Gly Gln Asp Gln Leu 
            420                 425                 430         


Ser Gln Asp Gly Ser Gly Tyr Glu Asn Pro Glu Asp Glu Pro Leu Gly 
        435                 440                 445             


Pro Glu Asp Glu Asp Ser Phe Ser Asn Ala Glu Ser Tyr Glu Asn Glu 
    450                 455                 460                 


Asp Glu Glu Leu Thr Gln Pro Val Ala Arg Thr Met Asp Phe Leu Ser 
465                 470                 475                 480 


Pro His Gly Ser Ala Trp Asp Pro Ser Arg Glu Ala Thr Ser Leu Gly 
                485                 490                 495     


Ser Gln Ser Tyr Glu Asp Met Arg Gly Ile Leu Tyr Ala Ala Pro Gln 
            500                 505                 510         


Leu Arg Ser Ile Arg Gly Gln Pro Gly Pro Asn His Glu Glu Asp Ala 
        515                 520                 525             


Asp Ser Tyr Glu Asn Met Asp Asn Pro Asp Gly Pro Asp Pro Ala Trp 
    530                 535                 540                 


Gly Gly Gly Gly Arg Met Gly Thr Trp Ser Thr Arg 
545                 550                 555     


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19Rop primer

<400>  8
aggaagatat cgccacctac t                                                 21


<210>  9
<211>  245
<212>  PRT
<213>  Homo sapiens

<400>  9

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly 
            100                 105                 110         


Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys 
        115                 120                 125             


Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 
    130                 135                 140                 


Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser 
145                 150                 155                 160 


Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 
                165                 170                 175     


Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu 
            180                 185                 190         


Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 
        195                 200                 205             


Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr 
    210                 215                 220                 


Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  10
<211>  735
<212>  DNA
<213>  Homo sapiens

<400>  10
gacatccaga tgacccagac cacctccagc ctgagcgcca gcctgggcga ccgggtgacc       60

atcagctgcc gggccagcca ggacatcagc aagtacctga actggtatca gcagaagccc      120

gacggcaccg tcaagctgct gatctaccac accagccggc tgcacagcgg cgtgcccagc      180

cggtttagcg gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag      240

gaagatatcg ccacctactt ttgccagcag ggcaacacac tgccctacac ctttggcggc      300

ggaacaaagc tggaaatcac cggcagcacc tccggcagcg gcaagcctgg cagcggcgag      360

ggcagcacca agggcgaggt gaagctgcag gaaagcggcc ctggcctggt ggcccccagc      420

cagagcctga gcgtgacctg caccgtgagc ggcgtgagcc tgcccgacta cggcgtgagc      480

tggatccggc agccccccag gaagggcctg gaatggctgg gcgtgatctg gggcagcgag      540

accacctact acaacagcgc cctgaagagc cggctgacca tcatcaagga caacagcaag      600

agccaggtgt tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc      660

gccaagcact actactacgg cggcagctac gccatggact actggggcca gggcaccagc      720

gtgaccgtga gcagc                                                       735


<210>  11
<211>  297
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Thr Thr Pro Arg Asn Ser Val Asn Gly Thr Phe Pro Ala Glu Pro 
1               5                   10                  15      


Met Lys Gly Pro Ile Ala Met Gln Ser Gly Pro Lys Pro Leu Phe Arg 
            20                  25                  30          


Arg Met Ser Ser Leu Val Gly Pro Thr Gln Ser Phe Phe Met Arg Glu 
        35                  40                  45              


Ser Lys Thr Leu Gly Ala Val Gln Ile Met Asn Gly Leu Phe His Ile 
    50                  55                  60                  


Ala Leu Gly Gly Leu Leu Met Ile Pro Ala Gly Ile Tyr Ala Pro Ile 
65                  70                  75                  80  


Cys Val Thr Val Trp Tyr Pro Leu Trp Gly Gly Ile Met Tyr Ile Ile 
                85                  90                  95      


Ser Gly Ser Leu Leu Ala Ala Thr Glu Lys Asn Ser Arg Lys Cys Leu 
            100                 105                 110         


Val Lys Gly Lys Met Ile Met Asn Ser Leu Ser Leu Phe Ala Ala Ile 
        115                 120                 125             


Ser Gly Met Ile Leu Ser Ile Met Asp Ile Leu Asn Ile Lys Ile Ser 
    130                 135                 140                 


His Phe Leu Lys Met Glu Ser Leu Asn Phe Ile Arg Ala His Thr Pro 
145                 150                 155                 160 


Tyr Ile Asn Ile Tyr Asn Cys Glu Pro Ala Asn Pro Ser Glu Lys Asn 
                165                 170                 175     


Ser Pro Ser Thr Gln Tyr Cys Tyr Ser Ile Gln Ser Leu Phe Leu Gly 
            180                 185                 190         


Ile Leu Ser Val Met Leu Ile Phe Ala Phe Phe Gln Glu Leu Val Ile 
        195                 200                 205             


Ala Gly Ile Val Glu Asn Glu Trp Lys Arg Thr Cys Ser Arg Pro Lys 
    210                 215                 220                 


Ser Asn Ile Val Leu Leu Ser Ala Glu Glu Lys Lys Glu Gln Thr Ile 
225                 230                 235                 240 


Glu Ile Lys Glu Glu Val Val Gly Leu Thr Glu Thr Ser Ser Gln Pro 
                245                 250                 255     


Lys Asn Glu Glu Asp Ile Glu Ile Ile Pro Ile Gln Glu Glu Glu Glu 
            260                 265                 270         


Glu Glu Thr Glu Thr Asn Phe Pro Glu Pro Pro Gln Asp Gln Glu Ser 
        275                 280                 285             


Ser Pro Ile Glu Asn Asp Ser Ser Pro 
    290                 295         


<210>  12
<211>  84
<212>  DNA
<213>  Homo sapiens

<400>  12
atgttctggg tgctggtggt ggtcggaggc gtgctggcct gctacagcct gctggtcacc       60

gtggccttca tcatcttttg ggtg                                              84


<210>  13
<211>  28
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
1               5                   10                  15      


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25              


<210>  14
<211>  84
<212>  DNA
<213>  Homo sapiens

<400>  14
atgttctggg tgctggtggt ggtgggcggg gtgctggcct gctacagcct gctggtgaca       60

gtggccttca tcatcttttg ggtg                                              84


<210>  15
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  15

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  16
<211>  336
<212>  DNA
<213>  Homo sapiens

<400>  16
cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca gaatcagctg       60

tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa gcggagaggc      120

cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg cctgtataac      180

gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg      240

aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac caaggatacc      300

tacgacgccc tgcacatgca ggccctgccc ccaagg                                336


<210>  17
<211>  109
<212>  PRT
<213>  Homo sapiens

<400>  17

Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
1               5                   10                  15      


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
            20                  25                  30          


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
        35                  40                  45              


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
    50                  55                  60                  


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
65                  70                  75                  80  


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
                85                  90                  95      


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 


<210>  18
<211>  327
<212>  DNA
<213>  Homo sapiens

<400>  18
ttcagcagaa gcgccgacgc ccctgcctac cagcagggcc agaatcagct gtacaacgag       60

ctgaacctgg gcagaaggga agagtacgac gtcctggata agcggagagg ccgggaccct      120

gagatgggcg gcaagcctcg gcggaagaac ccccaggaag gcctgtataa cgaactgcag      180

aaagacaaga tggccgaggc ctacagcgag atcggcatga agggcgagcg gaggcggggc      240

aagggccacg acggcctgta tcagggcctg tccaccgcca ccaaggatac ctacgacgcc      300

ctgcacatgc aggccctgcc cccaagg                                          327


<210>  19
<211>  110
<212>  PRT
<213>  Homo sapiens

<400>  19

Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  20
<211>  330
<212>  DNA
<213>  Homo sapiens

<400>  20
gcccccgagt tcctgggcgg acccagcgtg ttcctgttcc cccccaagcc caaggacacc       60

ctgatgatca gccggacccc cgaggtgacc tgcgtggtgg tggacgtgag ccaggaagat      120

cccgaggtcc agttcaattg gtacgtggac ggcgtggaag tgcacaacgc caagaccaag      180

cccagagagg aacagttcaa cagcacctac cgggtggtgt ctgtgctgac cgtgctgcac      240

caggactggc tgaacggcaa agaatacaag tgcaaggtgt ccaacaaggg cctgcccagc      300

agcatcgaaa agaccatcag caaggccaag                                       330


<210>  21
<211>  321
<212>  DNA
<213>  Homo sapiens

<400>  21
ggccagcctc gcgagcccca ggtgtacacc ctgcctccct cccaggaaga gatgaccaag       60

aaccaggtgt ccctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag      120

tgggagagca acggccagcc tgagaacaac tacaagacca cccctcccgt gctggacagc      180

gacggcagct tcttcctgta cagccggctg accgtggaca agagccggtg gcaggaaggc      240

aacgtcttta gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc      300

ctgagcctgt ccctgggcaa g                                                321


<210>  22
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  22

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu 
1               5                   10                  15      


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
            100                 105         


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV primer

<400>  23
tagcggtttg actcacgg                                                     18


<210>  24
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CoE1 ori primer

<400>  24
caggtatccg gtaagcgg                                                     18


<210>  25
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delU3 primer

<400>  25
ccgtaccttt aagaccaatg acttac                                            26


<210>  26
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EF1p primer

<400>  26
tcgcaacggg tttgcc                                                       16


<210>  27
<211>  1074
<212>  DNA
<213>  Homo sapiens

<400>  27
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata      120

aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc      180

ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa      240

ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct      300

gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag      360

caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc      420

tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat      480

gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata      540

agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc      600

cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga      660

ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag      720

aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc      780

acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc      840

gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca      900

gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca      960

ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg     1020

ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gtga           1074


<210>  28
<211>  357
<212>  PRT
<213>  Homo sapiens

<400>  28

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 
            20                  25                  30          


Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 
        35                  40                  45              


Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 
    50                  55                  60                  


Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 
65                  70                  75                  80  


Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 
                85                  90                  95      


Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 
            100                 105                 110         


Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 
        115                 120                 125             


Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 
    130                 135                 140                 


Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 
145                 150                 155                 160 


Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 
                165                 170                 175     


Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 
            180                 185                 190         


Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 
        195                 200                 205             


Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 
    210                 215                 220                 


Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 
225                 230                 235                 240 


Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 
                245                 250                 255     


Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 
            260                 265                 270         


His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 
        275                 280                 285             


Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 
    290                 295                 300                 


Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 
305                 310                 315                 320 


Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala 
                325                 330                 335     


Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly 
            340                 345                 350         


Ile Gly Leu Phe Met 
        355         


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EGFRt primer

<400>  29
atgcttctcc tggtgacaag                                                   20


<210>  30
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible Linker

<400>  30

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  31
<211>  66
<212>  DNA
<213>  Homo sapiens

<400>  31
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg       60

atcccc                                                                  66


<210>  32
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GMCSFRss

<400>  32

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  33
<211>  2529
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GMCSFRss-CD19scFv-IgG4hinge-CD28tm-41BB-Zeta-T2A-EGFRt

<400>  33
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg       60

atccccgaca tccagatgac ccagaccacc tccagcctga gcgccagcct gggcgaccgg      120

gtgaccatca gctgccgggc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aagcccgacg gcaccgtcaa gctgctgatc taccacacca gccggctgca cagcggcgtg      240

cccagccggt ttagcggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgccac ctacttttgc cagcagggca acacactgcc ctacaccttt      360

ggcggcggaa caaagctgga aatcaccggc agcacctccg gcagcggcaa gcctggcagc      420

ggcgagggca gcaccaaggg cgaggtgaag ctgcaggaaa gcggccctgg cctggtggcc      480

cccagccaga gcctgagcgt gacctgcacc gtgagcggcg tgagcctgcc cgactacggc      540

gtgagctgga tccggcagcc ccccaggaag ggcctggaat ggctgggcgt gatctggggc      600

agcgagacca cctactacaa cagcgccctg aagagccggc tgaccatcat caaggacaac      660

agcaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

accagcgtga ccgtgagcag cgagagcaag tacggaccgc cctgcccccc ttgccctatg      840

ttctgggtgc tggtggtggt cggaggcgtg ctggcctgct acagcctgct ggtcaccgtg      900

gccttcatca tcttttgggt gaaacggggc agaaagaaac tcctgtatat attcaaacaa      960

ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca     1020

gaagaagaag aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct     1080

gcctaccagc agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag     1140

tacgacgtcc tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg     1200

aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac     1260

agcgagatcg gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag     1320

ggcctgtcca ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca     1380

aggctcgagg gcggcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag     1440

aatcccggcc ctaggatgct tctcctggtg acaagccttc tgctctgtga gttaccacac     1500

ccagcattcc tcctgatccc acgcaaagtg tgtaacggaa taggtattgg tgaatttaaa     1560

gactcactct ccataaatgc tacgaatatt aaacacttca aaaactgcac ctccatcagt     1620

ggcgatctcc acatcctgcc ggtggcattt aggggtgact ccttcacaca tactcctcct     1680

ctggatccac aggaactgga tattctgaaa accgtaaagg aaatcacagg gtttttgctg     1740

attcaggctt ggcctgaaaa caggacggac ctccatgcct ttgagaacct agaaatcata     1800

cgcggcagga ccaagcaaca tggtcagttt tctcttgcag tcgtcagcct gaacataaca     1860

tccttgggat tacgctccct caaggagata agtgatggag atgtgataat ttcaggaaac     1920

aaaaatttgt gctatgcaaa tacaataaac tggaaaaaac tgtttgggac ctccggtcag     1980

aaaaccaaaa ttataagcaa cagaggtgaa aacagctgca aggccacagg ccaggtctgc     2040

catgccttgt gctcccccga gggctgctgg ggcccggagc ccagggactg cgtctcttgc     2100

cggaatgtca gccgaggcag ggaatgcgtg gacaagtgca accttctgga gggtgagcca     2160

agggagtttg tggagaactc tgagtgcata cagtgccacc cagagtgcct gcctcaggcc     2220

atgaacatca cctgcacagg acggggacca gacaactgta tccagtgtgc ccactacatt     2280

gacggccccc actgcgtcaa gacctgcccg gcaggagtca tgggagaaaa caacaccctg     2340

gtctggaagt acgcagacgc cggccatgtg tgccacctgt gccatccaaa ctgcacctac     2400

ggatgcactg ggccaggtct tgaaggctgt ccaacgaatg ggcctaagat cccgtccatc     2460

gccactggga tggtgggggc cctcctcttg ctgctggtgg tggccctggg gatcggcctc     2520

ttcatgtga                                                             2529


<210>  34
<211>  842
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GMCSFRss-CD19scFv-IgG4hinge-CD28tm-41BB-Zeta-T2A-EGFRt

<400>  34

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly 
            260                 265                 270         


Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly 
        275                 280                 285             


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
    290                 295                 300                 


Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
305                 310                 315                 320 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                325                 330                 335     


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            340                 345                 350         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
385                 390                 395                 400 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                405                 410                 415     


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            420                 425                 430         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        435                 440                 445             


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly 
    450                 455                 460                 


Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
465                 470                 475                 480 


Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys 
                485                 490                 495     


Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn 
            500                 505                 510         


Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr 
        515                 520                 525             


Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His 
    530                 535                 540                 


Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro 
545                 550                 555                 560 


Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr 
                565                 570                 575     


Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His 
            580                 585                 590         


Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly 
        595                 600                 605             


Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu 
    610                 615                 620                 


Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn 
625                 630                 635                 640 


Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly 
                645                 650                 655     


Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser 
            660                 665                 670         


Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly 
        675                 680                 685             


Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser 
    690                 695                 700                 


Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro 
705                 710                 715                 720 


Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys 
                725                 730                 735     


Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn 
            740                 745                 750         


Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr 
        755                 760                 765             


Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr 
    770                 775                 780                 


Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr 
785                 790                 795                 800 


Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys 
                805                 810                 815     


Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu 
            820                 825                 830         


Val Val Ala Leu Gly Ile Gly Leu Phe Met 
        835                 840         


<210>  35
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GMCSFss Leader

<400>  35
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atccca                                                                  66


<210>  36
<211>  2529
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GMCSFss-Her2scFv-IgG4hinge-CD28tm-41BB-Zeta-T2A-EGFRt

<400>  36
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccagata tccagatgac ccagtccccg agctccctgt ccgcctctgt gggcgatagg      120

gtcaccatca cctgccgtgc cagtcaggat gtgaatactg ctgtagcctg gtatcaacag      180

aaaccaggaa aagctccgaa actactgatt tactcggcat ccttcctcta ctctggagtc      240

ccttctcgct tctctggttc cagatctggg acggatttca ctctgaccat cagcagtctg      300

cagccggaag acttcgcaac ttattactgt cagcaacatt atactactcc tcccacgttc      360

ggacagggta ccaaggtgga gatcaaaggc agtactagcg gcggtggctc cgggggcgga      420

tccggtgggg gcggcagcag cgaggttcag ctggtggagt ctggcggtgg cctggtgcag      480

ccagggggct cactccgttt gtcctgtgca gcttctggct tcaacattaa agacacctat      540

atacactggg tgcgtcaggc cccgggtaag ggcctggaat gggttgcaag gatttatcct      600

acgaatggtt atactagata tgccgatagc gtcaagggcc gtttcactat aagcgcagac      660

acatccaaaa acacagccta cctgcagatg aacagcctgc gtgctgagga cactgccgtc      720

tattattgtt ctagatgggg aggggacggc ttctatgcta tggactactg gggtcaagga      780

accctggtca ccgtctcgag tgagagcaag tacggaccgc cctgcccccc ttgccctatg      840

ttctgggtgc tggtggtggt cggaggcgtg ctggcctgct acagcctgct ggtcaccgtg      900

gccttcatca tcttttgggt gaaacggggc agaaagaaac tcctgtatat attcaaacaa      960

ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca     1020

gaagaagaag aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct     1080

gcctaccagc agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag     1140

tacgacgtcc tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg     1200

aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac     1260

agcgagatcg gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag     1320

ggcctgtcca ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca     1380

aggctcgagg gcggcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag     1440

aatcccggcc ctaggatgct tctcctggtg acaagccttc tgctctgtga gttaccacac     1500

ccagcattcc tcctgatccc acgcaaagtg tgtaacggaa taggtattgg tgaatttaaa     1560

gactcactct ccataaatgc tacgaatatt aaacacttca aaaactgcac ctccatcagt     1620

ggcgatctcc acatcctgcc ggtggcattt aggggtgact ccttcacaca tactcctcct     1680

ctggatccac aggaactgga tattctgaaa accgtaaagg aaatcacagg gtttttgctg     1740

attcaggctt ggcctgaaaa caggacggac ctccatgcct ttgagaacct agaaatcata     1800

cgcggcagga ccaagcaaca tggtcagttt tctcttgcag tcgtcagcct gaacataaca     1860

tccttgggat tacgctccct caaggagata agtgatggag atgtgataat ttcaggaaac     1920

aaaaatttgt gctatgcaaa tacaataaac tggaaaaaac tgtttgggac ctccggtcag     1980

aaaaccaaaa ttataagcaa cagaggtgaa aacagctgca aggccacagg ccaggtctgc     2040

catgccttgt gctcccccga gggctgctgg ggcccggagc ccagggactg cgtctcttgc     2100

cggaatgtca gccgaggcag ggaatgcgtg gacaagtgca accttctgga gggtgagcca     2160

agggagtttg tggagaactc tgagtgcata cagtgccacc cagagtgcct gcctcaggcc     2220

atgaacatca cctgcacagg acggggacca gacaactgta tccagtgtgc ccactacatt     2280

gacggccccc actgcgtcaa gacctgcccg gcaggagtca tgggagaaaa caacaccctg     2340

gtctggaagt acgcagacgc cggccatgtg tgccacctgt gccatccaaa ctgcacctac     2400

ggatgcactg ggccaggtct tgaaggctgt ccaacgaatg ggcctaagat cccgtccatc     2460

gccactggga tggtgggggc cctcctcttg ctgctggtgg tggccctggg gatcggcctc     2520

ttcatgtga                                                             2529


<210>  37
<211>  2850
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Her 2 construct-intermediate spacer

<400>  37
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccagata tccagatgac ccagtccccg agctccctgt ccgcctctgt gggcgatagg      120

gtcaccatca cctgccgtgc cagtcaggat gtgaatactg ctgtagcctg gtatcaacag      180

aaaccaggaa aagctccgaa actactgatt tactcggcat ccttcctcta ctctggagtc      240

ccttctcgct tctctggttc cagatctggg acggatttca ctctgaccat cagcagtctg      300

cagccggaag acttcgcaac ttattactgt cagcaacatt atactactcc tcccacgttc      360

ggacagggta ccaaggtgga gatcaaaggc agtactagcg gcggtggctc cgggggcgga      420

tccggtgggg gcggcagcag cgaggttcag ctggtggagt ctggcggtgg cctggtgcag      480

ccagggggct cactccgttt gtcctgtgca gcttctggct tcaacattaa agacacctat      540

atacactggg tgcgtcaggc cccgggtaag ggcctggaat gggttgcaag gatttatcct      600

acgaatggtt atactagata tgccgatagc gtcaagggcc gtttcactat aagcgcagac      660

acatccaaaa acacagccta cctgcagatg aacagcctgc gtgctgagga cactgccgtc      720

tattattgtt ctagatgggg aggggacggc ttctatgcta tggactactg gggtcaagga      780

accctggtca ccgtctcgag tgagagcaag tacggaccgc cctgcccccc ttgccctggc      840

cagcctagag aaccccaggt gtacaccctg cctcccagcc aggaagagat gaccaagaac      900

caggtgtccc tgacctgcct ggtcaaaggc ttctacccca gcgatatcgc cgtggaatgg      960

gagagcaacg gccagcccga gaacaactac aagaccaccc cccctgtgct ggacagcgac     1020

ggcagcttct tcctgtactc ccggctgacc gtggacaaga gccggtggca ggaaggcaac     1080

gtcttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagtccctg     1140

agcctgagcc tgggcaagat gttctgggtg ctggtggtgg tcggaggcgt gctggcctgc     1200

tacagcctgc tggtcaccgt ggccttcatc atcttttggg tgaaacgggg cagaaagaaa     1260

ctcctgtata tattcaaaca accatttatg agaccagtac aaactactca agaggaagat     1320

ggctgtagct gccgatttcc agaagaagaa gaaggaggat gtgaactgcg ggtgaagttc     1380

agcagaagcg ccgacgcccc tgcctaccag cagggccaga atcagctgta caacgagctg     1440

aacctgggca gaagggaaga gtacgacgtc ctggataagc ggagaggccg ggaccctgag     1500

atgggcggca agcctcggcg gaagaacccc caggaaggcc tgtataacga actgcagaaa     1560

gacaagatgg ccgaggccta cagcgagatc ggcatgaagg gcgagcggag gcggggcaag     1620

ggccacgacg gcctgtatca gggcctgtcc accgccacca aggataccta cgacgccctg     1680

cacatgcagg ccctgccccc aaggctcgag ggcggcggag agggcagagg aagtcttcta     1740

acatgcggtg acgtggagga gaatcccggc cctaggatgc ttctcctggt gacaagcctt     1800

ctgctctgtg agttaccaca cccagcattc ctcctgatcc cacgcaaagt gtgtaacgga     1860

ataggtattg gtgaatttaa agactcactc tccataaatg ctacgaatat taaacacttc     1920

aaaaactgca cctccatcag tggcgatctc cacatcctgc cggtggcatt taggggtgac     1980

tccttcacac atactcctcc tctggatcca caggaactgg atattctgaa aaccgtaaag     2040

gaaatcacag ggtttttgct gattcaggct tggcctgaaa acaggacgga cctccatgcc     2100

tttgagaacc tagaaatcat acgcggcagg accaagcaac atggtcagtt ttctcttgca     2160

gtcgtcagcc tgaacataac atccttggga ttacgctccc tcaaggagat aagtgatgga     2220

gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa atacaataaa ctggaaaaaa     2280

ctgtttggga cctccggtca gaaaaccaaa attataagca acagaggtga aaacagctgc     2340

aaggccacag gccaggtctg ccatgccttg tgctcccccg agggctgctg gggcccggag     2400

cccagggact gcgtctcttg ccggaatgtc agccgaggca gggaatgcgt ggacaagtgc     2460

aaccttctgg agggtgagcc aagggagttt gtggagaact ctgagtgcat acagtgccac     2520

ccagagtgcc tgcctcaggc catgaacatc acctgcacag gacggggacc agacaactgt     2580

atccagtgtg cccactacat tgacggcccc cactgcgtca agacctgccc ggcaggagtc     2640

atgggagaaa acaacaccct ggtctggaag tacgcagacg ccggccatgt gtgccacctg     2700

tgccatccaa actgcaccta cggatgcact gggccaggtc ttgaaggctg tccaacgaat     2760

gggcctaaga tcccgtccat cgccactggg atggtggggg ccctcctctt gctgctggtg     2820

gtggccctgg ggatcggcct cttcatgtga                                      2850


<210>  38
<211>  3180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Her 2 construct-long spacer

<400>  38
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccagata tccagatgac ccagtccccg agctccctgt ccgcctctgt gggcgatagg      120

gtcaccatca cctgccgtgc cagtcaggat gtgaatactg ctgtagcctg gtatcaacag      180

aaaccaggaa aagctccgaa actactgatt tactcggcat ccttcctcta ctctggagtc      240

ccttctcgct tctctggttc cagatctggg acggatttca ctctgaccat cagcagtctg      300

cagccggaag acttcgcaac ttattactgt cagcaacatt atactactcc tcccacgttc      360

ggacagggta ccaaggtgga gatcaaaggc agtactagcg gcggtggctc cgggggcgga      420

tccggtgggg gcggcagcag cgaggttcag ctggtggagt ctggcggtgg cctggtgcag      480

ccagggggct cactccgttt gtcctgtgca gcttctggct tcaacattaa agacacctat      540

atacactggg tgcgtcaggc cccgggtaag ggcctggaat gggttgcaag gatttatcct      600

acgaatggtt atactagata tgccgatagc gtcaagggcc gtttcactat aagcgcagac      660

acatccaaaa acacagccta cctgcagatg aacagcctgc gtgctgagga cactgccgtc      720

tattattgtt ctagatgggg aggggacggc ttctatgcta tggactactg gggtcaagga      780

accctggtca ccgtctcgag tgagagcaag tacggaccgc cctgcccccc ttgccctgcc      840

cccgagttcc tgggcggacc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg      900

atgatcagcc ggacccccga ggtgacctgc gtggtggtgg acgtgagcca ggaagatccc      960

gaggtccagt tcaattggta cgtggacggc gtggaagtgc acaacgccaa gaccaagccc     1020

agagaggaac agttcaacag cacctaccgg gtggtgtctg tgctgaccgt gctgcaccag     1080

gactggctga acggcaaaga atacaagtgc aaggtgtcca acaagggcct gcccagcagc     1140

atcgaaaaga ccatcagcaa ggccaagggc cagcctcgcg agccccaggt gtacaccctg     1200

cctccctccc aggaagagat gaccaagaac caggtgtccc tgacctgcct ggtgaagggc     1260

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcctga gaacaactac     1320

aagaccaccc ctcccgtgct ggacagcgac ggcagcttct tcctgtacag ccggctgacc     1380

gtggacaaga gccggtggca ggaaggcaac gtctttagct gcagcgtgat gcacgaggcc     1440

ctgcacaacc actacaccca gaagagcctg agcctgtccc tgggcaagat gttctgggtg     1500

ctggtggtgg tgggcggggt gctggcctgc tacagcctgc tggtgacagt ggccttcatc     1560

atcttttggg tgaaacgggg cagaaagaaa ctcctgtata tattcaaaca accatttatg     1620

agaccagtac aaactactca agaggaagat ggctgtagct gccgatttcc agaagaagaa     1680

gaaggaggat gtgaactgcg ggtgaagttc agcagaagcg ccgacgcccc tgcctaccag     1740

cagggccaga atcagctgta caacgagctg aacctgggca gaagggaaga gtacgacgtc     1800

ctggataagc ggagaggccg ggaccctgag atgggcggca agcctcggcg gaagaacccc     1860

caggaaggcc tgtataacga actgcagaaa gacaagatgg ccgaggccta cagcgagatc     1920

ggcatgaagg gcgagcggag gcggggcaag ggccacgacg gcctgtatca gggcctgtcc     1980

accgccacca aggataccta cgacgccctg cacatgcagg ccctgccccc aaggctcgag     2040

ggcggcggag agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc     2100

cctaggatgc ttctcctggt gacaagcctt ctgctctgtg agttaccaca cccagcattc     2160

ctcctgatcc cacgcaaagt gtgtaacgga ataggtattg gtgaatttaa agactcactc     2220

tccataaatg ctacgaatat taaacacttc aaaaactgca cctccatcag tggcgatctc     2280

cacatcctgc cggtggcatt taggggtgac tccttcacac atactcctcc tctggatcca     2340

caggaactgg atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct     2400

tggcctgaaa acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg     2460

accaagcaac atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga     2520

ttacgctccc tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg     2580

tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa     2640

attataagca acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg     2700

tgctcccccg agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc     2760

agccgaggca gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt     2820

gtggagaact ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc     2880

acctgcacag gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc     2940

cactgcgtca agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag     3000

tacgcagacg ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact     3060

gggccaggtc ttgaaggctg tccaacgaat gggcctaaga tcccgtccat cgccactggg     3120

atggtggggg ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgtga     3180


<210>  39
<211>  735
<212>  DNA
<213>  Homo sapiens

<400>  39
gatatccaga tgacccagtc cccgagctcc ctgtccgcct ctgtgggcga tagggtcacc       60

atcacctgcc gtgccagtca ggatgtgaat actgctgtag cctggtatca acagaaacca      120

ggaaaagctc cgaaactact gatttactcg gcatccttcc tctactctgg agtcccttct      180

cgcttctctg gttccagatc tgggacggat ttcactctga ccatcagcag tctgcagccg      240

gaagacttcg caacttatta ctgtcagcaa cattatacta ctcctcccac gttcggacag      300

ggtaccaagg tggagatcaa aggcagtact agcggcggtg gctccggggg cggatccggt      360

gggggcggca gcagcgaggt tcagctggtg gagtctggcg gtggcctggt gcagccaggg      420

ggctcactcc gtttgtcctg tgcagcttct ggcttcaaca ttaaagacac ctatatacac      480

tgggtgcgtc aggccccggg taagggcctg gaatgggttg caaggattta tcctacgaat      540

ggttatacta gatatgccga tagcgtcaag ggccgtttca ctataagcgc agacacatcc      600

aaaaacacag cctacctgca gatgaacagc ctgcgtgctg aggacactgc cgtctattat      660

tgttctagat ggggagggga cggcttctat gctatggact actggggtca aggaaccctg      720

gtcaccgtct cgagt                                                       735


<210>  40
<211>  753
<212>  DNA
<213>  Homo sapiens

<400>  40
gcattcctcc tgatcccaga tatccagatg acccagtccc cgagctccct gtccgcctct       60

gtgggcgata gggtcaccat cacctgccgt gccagtcagg atgtgaatac tgctgtagcc      120

tggtatcaac agaaaccagg aaaagctccg aaactactga tttactcggc atccttcctc      180

tactctggag tcccttctcg cttctctggt tccagatctg ggacggattt cactctgacc      240

atcagcagtc tgcagccgga agacttcgca acttattact gtcagcaaca ttatactact      300

cctcccacgt tcggacaggg taccaaggtg gagatcaaag gcagtactag cggcggtggc      360

tccgggggcg gatccggtgg gggcggcagc agcgaggttc agctggtgga gtctggcggt      420

ggcctggtgc agccaggggg ctcactccgt ttgtcctgtg cagcttctgg cttcaacatt      480

aaagacacct atatacactg ggtgcgtcag gccccgggta agggcctgga atgggttgca      540

aggatttatc ctacgaatgg ttatactaga tatgccgata gcgtcaaggg ccgtttcact      600

ataagcgcag acacatccaa aaacacagcc tacctgcaga tgaacagcct gcgtgctgag      660

gacactgccg tctattattg ttctagatgg ggaggggacg gcttctatgc tatggactac      720

tggggtcaag gaaccctggt caccgtctcg agt                                   753


<210>  41
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hinge Spacer

<400>  41
gagagcaagt acggaccgcc ctgcccccct tgccctggcc agcctagaga accccaggtg       60

tacaccctgc ctcccagcca ggaagagatg accaagaacc aggtgtccct gacctgcctg      120

gtcaaaggct tctaccccag cgatatcgcc gtggaatggg agagcaacgg ccagcccgag      180

aacaactaca agaccacccc ccctgtgctg gacagcgacg gcagcttctt cctgtactcc      240

cggctgaccg tggacaagag ccggtggcag gaaggcaacg tcttcagctg cagcgtgatg      300

cacgaggccc tgcacaacca ctacacccag aagtccctga gcctgagcct gggcaag         357


<210>  42
<211>  356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hinge/Spacer

<400>  42
taggaccgcc ctgcccccct tgccctgccc ccgagttcct gggcggaccc agcgtgttcc       60

tgttcccccc caagcccaag gacaccctga tgatcagccg gacccccgag gtgacctgcg      120

tggtggtgga cgtgagccag gaagatcccg aggtccagtt caattggtac gtggacggcg      180

tggaagtgca caacgccaag accaagccca gagaggaaca gttcaacagc acctaccggg      240

tggtgtctgt gctgaccgtg ctgcaccagg actggctgaa cggcaaagaa tacaagtgca      300

aggtgtccaa caagggcctg cccagcagca tcgaaaagac catcagcaag gccaag          356


<210>  43
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hinge/Spacer

<400>  43
tacggaccgc cctgcccccc ttgccctggc cagcctcgcg agccccaggt gtacaccctg       60

cctccctccc aggaagagat gaccaagaac caggtgtccc tgacctgcct ggtgaagggc      120

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcctga gaacaactac      180

aagaccaccc ctcccgtgct ggacagcgac ggcagcttct tcctgtacag ccggctgacc      240

gtggacaaga gccggtggca ggaaggcaac gtctttagct gcagcgtgat gcacgaggcc      300

ctgcacaacc actacaccca gaagagcctg agcctgtccc tgggcaag                   348


<210>  44
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  44

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  45
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  45

Glu Leu Lys Thr Pro Leu Gly Asp Thr His Thr Cys Pro Arg Cys Pro 
1               5                   10                  15      


<210>  46
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  46

Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
1               5                   10                  15  


<210>  47
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  47

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  48
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  48

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  49
<211>  27
<212>  DNA
<213>  Homo sapiens

<400>  49
tacggaccgc cctgcccccc ttgccct                                           27


<210>  50
<211>  36
<212>  DNA
<213>  Homo sapiens

<400>  50
gaatctaagt acggaccgcc ctgcccccct tgccct                                 36


<210>  51
<211>  36
<212>  DNA
<213>  Homo sapiens

<400>  51
gagagcaagt acggaccgcc ctgcccccct tgccct                                 36


<210>  52
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Intermediate Spacer

<400>  52

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg 
1               5                   10                  15      


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
            20                  25                  30          


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        35                  40                  45              


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    50                  55                  60                  


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
65                  70                  75                  80  


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
                85                  90                  95      


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            100                 105                 110         


Leu Ser Leu Ser Leu Gly Lys 
        115                 


<210>  53
<211>  838
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader _R11- Hinge- CD28tm/41BB-Z-T2A-tEGFR

<400>  53

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Ser Val Lys Glu Ser Glu Gly Asp Leu 
            20                  25                  30          


Val Thr Pro Ala Gly Asn Leu Thr Leu Thr Cys Thr Ala Ser Gly Ser 
        35                  40                  45              


Asp Ile Asn Asp Tyr Pro Ile Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Phe Ile Asn Ser Gly Gly Ser Thr Trp Tyr 
65                  70                  75                  80  


Ala Ser Trp Val Lys Gly Arg Phe Thr Ile Ser Arg Thr Ser Thr Thr 
                85                  90                  95      


Val Asp Leu Lys Met Thr Ser Leu Thr Thr Asp Asp Thr Ala Thr Tyr 
            100                 105                 110         


Phe Cys Ala Arg Gly Tyr Ser Thr Tyr Tyr Gly Asp Phe Asn Ile Trp 
        115                 120                 125             


Gly Pro Gly Thr Leu Val Thr Ile Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Val Met Thr Gln Thr 
145                 150                 155                 160 


Pro Ser Ser Thr Ser Gly Ala Val Gly Gly Thr Val Thr Ile Asn Cys 
                165                 170                 175     


Gln Ala Ser Gln Ser Ile Asp Ser Asn Leu Ala Trp Phe Gln Gln Lys 
            180                 185                 190         


Pro Gly Gln Pro Pro Thr Leu Leu Ile Tyr Arg Ala Ser Asn Leu Ala 
        195                 200                 205             


Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Glu Tyr 
    210                 215                 220                 


Thr Leu Thr Ile Ser Gly Val Gln Arg Glu Asp Ala Ala Thr Tyr Tyr 
225                 230                 235                 240 


Cys Leu Gly Gly Val Gly Asn Val Ser Tyr Arg Thr Ser Phe Gly Gly 
                245                 250                 255     


Gly Thr Glu Val Val Val Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro 
            260                 265                 270         


Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
        275                 280                 285             


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys 
    290                 295                 300                 


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
305                 310                 315                 320 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
                325                 330                 335     


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
385                 390                 395                 400 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                405                 410                 415     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            420                 425                 430         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        435                 440                 445             


Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly 
    450                 455                 460                 


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
465                 470                 475                 480 


Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His 
                485                 490                 495     


Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile 
            500                 505                 510         


Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His 
        515                 520                 525             


Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val 
    530                 535                 540                 


Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln 
545                 550                 555                 560 


Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu 
                565                 570                 575     


Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn 
            580                 585                 590         


Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu 
        595                 600                 605             


Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys 
    610                 615                 620                 


Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys 
625                 630                 635                 640 


Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln 
                645                 650                 655     


Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr 
            660                 665                 670         


Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro 
        675                 680                 685             


Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu 
    690                 695                 700                 


Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val 
705                 710                 715                 720 


Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala 
                725                 730                 735     


Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys 
            740                 745                 750         


Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly 
        755                 760                 765             


Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly 
    770                 775                 780                 


His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly 
785                 790                 795                 800 


Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile 
                805                 810                 815     


Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu 
            820                 825                 830         


Gly Ile Gly Leu Phe Met 
        835             


<210>  54
<211>  1055
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader _R11- Hinge-CH2-CH3- CD28tm/41BB-Z-T2A-tEGFR

<400>  54

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Ser Val Lys Glu Ser Glu Gly Asp Leu 
            20                  25                  30          


Val Thr Pro Ala Gly Asn Leu Thr Leu Thr Cys Thr Ala Ser Gly Ser 
        35                  40                  45              


Asp Ile Asn Asp Tyr Pro Ile Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Phe Ile Asn Ser Gly Gly Ser Thr Trp Tyr 
65                  70                  75                  80  


Ala Ser Trp Val Lys Gly Arg Phe Thr Ile Ser Arg Thr Ser Thr Thr 
                85                  90                  95      


Val Asp Leu Lys Met Thr Ser Leu Thr Thr Asp Asp Thr Ala Thr Tyr 
            100                 105                 110         


Phe Cys Ala Arg Gly Tyr Ser Thr Tyr Tyr Gly Asp Phe Asn Ile Trp 
        115                 120                 125             


Gly Pro Gly Thr Leu Val Thr Ile Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Val Met Thr Gln Thr 
145                 150                 155                 160 


Pro Ser Ser Thr Ser Gly Ala Val Gly Gly Thr Val Thr Ile Asn Cys 
                165                 170                 175     


Gln Ala Ser Gln Ser Ile Asp Ser Asn Leu Ala Trp Phe Gln Gln Lys 
            180                 185                 190         


Pro Gly Gln Pro Pro Thr Leu Leu Ile Tyr Arg Ala Ser Asn Leu Ala 
        195                 200                 205             


Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Glu Tyr 
    210                 215                 220                 


Thr Leu Thr Ile Ser Gly Val Gln Arg Glu Asp Ala Ala Thr Tyr Tyr 
225                 230                 235                 240 


Cys Leu Gly Gly Val Gly Asn Val Ser Tyr Arg Thr Ser Phe Gly Gly 
                245                 250                 255     


Gly Thr Glu Val Val Val Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro 
            260                 265                 270         


Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe 
        275                 280                 285             


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
    290                 295                 300                 


Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe 
305                 310                 315                 320 


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
                325                 330                 335     


Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
            340                 345                 350         


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
        355                 360                 365             


Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala 
    370                 375                 380                 


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln 
385                 390                 395                 400 


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
                405                 410                 415     


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
            420                 425                 430         


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
        435                 440                 445             


Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu 
    450                 455                 460                 


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
465                 470                 475                 480 


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val 
                485                 490                 495     


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
            500                 505                 510         


Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu 
        515                 520                 525             


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
    530                 535                 540                 


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
545                 550                 555                 560 


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                565                 570                 575     


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            580                 585                 590         


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        595                 600                 605             


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    610                 615                 620                 


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
625                 630                 635                 640 


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                645                 650                 655     


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            660                 665                 670         


Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys 
        675                 680                 685             


Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr 
    690                 695                 700                 


Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro 
705                 710                 715                 720 


Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
                725                 730                 735     


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            740                 745                 750         


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        755                 760                 765             


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    770                 775                 780                 


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
785                 790                 795                 800 


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                805                 810                 815     


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            820                 825                 830         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        835                 840                 845             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    850                 855                 860                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
865                 870                 875                 880 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                885                 890                 895     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            900                 905                 910         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        915                 920                 925             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    930                 935                 940                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
945                 950                 955                 960 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                965                 970                 975     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            980                 985                 990         


Leu Val Trp Lys Tyr Ala Asp Ala  Gly His Val Cys His  Leu Cys His 
        995                 1000                 1005             


Pro Asn  Cys Thr Tyr Gly Cys  Thr Gly Pro Gly Leu  Glu Gly Cys 
    1010                 1015                 1020             


Pro Thr  Asn Gly Pro Lys Ile  Pro Ser Ile Ala Thr  Gly Met Val 
    1025                 1030                 1035             


Gly Ala  Leu Leu Leu Leu Leu  Val Val Ala Leu Gly  Ile Gly Leu 
    1040                 1045                 1050             


Phe Met  
    1055 


<210>  55
<211>  945
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader _R11- Hinge-CH3- CD28tm/41BB-Z-T2A-tEGFR

<400>  55

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Ser Val Lys Glu Ser Glu Gly Asp Leu 
            20                  25                  30          


Val Thr Pro Ala Gly Asn Leu Thr Leu Thr Cys Thr Ala Ser Gly Ser 
        35                  40                  45              


Asp Ile Asn Asp Tyr Pro Ile Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Phe Ile Asn Ser Gly Gly Ser Thr Trp Tyr 
65                  70                  75                  80  


Ala Ser Trp Val Lys Gly Arg Phe Thr Ile Ser Arg Thr Ser Thr Thr 
                85                  90                  95      


Val Asp Leu Lys Met Thr Ser Leu Thr Thr Asp Asp Thr Ala Thr Tyr 
            100                 105                 110         


Phe Cys Ala Arg Gly Tyr Ser Thr Tyr Tyr Gly Asp Phe Asn Ile Trp 
        115                 120                 125             


Gly Pro Gly Thr Leu Val Thr Ile Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Val Met Thr Gln Thr 
145                 150                 155                 160 


Pro Ser Ser Thr Ser Gly Ala Val Gly Gly Thr Val Thr Ile Asn Cys 
                165                 170                 175     


Gln Ala Ser Gln Ser Ile Asp Ser Asn Leu Ala Trp Phe Gln Gln Lys 
            180                 185                 190         


Pro Gly Gln Pro Pro Thr Leu Leu Ile Tyr Arg Ala Ser Asn Leu Ala 
        195                 200                 205             


Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Glu Tyr 
    210                 215                 220                 


Thr Leu Thr Ile Ser Gly Val Gln Arg Glu Asp Ala Ala Thr Tyr Tyr 
225                 230                 235                 240 


Cys Leu Gly Gly Val Gly Asn Val Ser Tyr Arg Thr Ser Phe Gly Gly 
                245                 250                 255     


Gly Thr Glu Val Val Val Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro 
            260                 265                 270         


Pro Cys Pro Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        275                 280                 285             


Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    290                 295                 300                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
305                 310                 315                 320 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                325                 330                 335     


Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp 
            340                 345                 350         


Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        355                 360                 365             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe 
    370                 375                 380                 


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
385                 390                 395                 400 


Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys 
                405                 410                 415     


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
            420                 425                 430         


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
        435                 440                 445             


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
    450                 455                 460                 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
465                 470                 475                 480 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
                485                 490                 495     


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
            500                 505                 510         


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        515                 520                 525             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    530                 535                 540                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
545                 550                 555                 560 


Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu 
                565                 570                 575     


Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu 
            580                 585                 590         


Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu 
        595                 600                 605             


Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp 
    610                 615                 620                 


Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr 
625                 630                 635                 640 


Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp 
                645                 650                 655     


Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu 
            660                 665                 670         


Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro 
        675                 680                 685             


Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg 
    690                 695                 700                 


Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu 
705                 710                 715                 720 


Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly 
                725                 730                 735     


Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile 
            740                 745                 750         


Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile 
        755                 760                 765             


Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His 
    770                 775                 780                 


Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys 
785                 790                 795                 800 


Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys 
                805                 810                 815     


Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys 
            820                 825                 830         


Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys 
        835                 840                 845             


Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp 
    850                 855                 860                 


Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn 
865                 870                 875                 880 


Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu 
                885                 890                 895     


Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly 
            900                 905                 910         


Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val 
        915                 920                 925             


Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe 
    930                 935                 940                 


Met 
945 


<210>  56
<211>  845
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader _R12 - CD28tm/41BB-Z-T2A-tEGFR

<400>  56

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Glu Gln Leu Val Glu Ser Gly Gly Arg 
            20                  25                  30          


Leu Val Thr Pro Gly Gly Ser Leu Thr Leu Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Phe Asp Phe Ser Ala Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Ile Ala Thr Ile Tyr Pro Ser Ser Gly Lys Thr 
65                  70                  75                  80  


Tyr Tyr Ala Thr Trp Val Asn Gly Arg Phe Thr Ile Ser Ser Asp Asn 
                85                  90                  95      


Ala Gln Asn Thr Val Asp Leu Gln Met Asn Ser Leu Thr Ala Ala Asp 
            100                 105                 110         


Arg Ala Thr Tyr Phe Cys Ala Arg Asp Ser Tyr Ala Asp Asp Gly Ala 
        115                 120                 125             


Leu Phe Asn Ile Trp Gly Pro Gly Thr Leu Val Thr Ile Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu 
145                 150                 155                 160 


Val Leu Thr Gln Ser Pro Ser Val Ser Ala Ala Leu Gly Ser Pro Ala 
                165                 170                 175     


Lys Ile Thr Cys Thr Leu Ser Ser Ala His Lys Thr Asp Thr Ile Asp 
            180                 185                 190         


Trp Tyr Gln Gln Leu Gln Gly Glu Ala Pro Arg Tyr Leu Met Gln Val 
        195                 200                 205             


Gln Ser Asp Gly Ser Tyr Thr Lys Arg Pro Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Ser Ser Gly Ala Asp Arg Tyr Leu Ile Ile Pro Ser Val 
225                 230                 235                 240 


Gln Ala Asp Asp Glu Ala Asp Tyr Tyr Cys Gly Ala Asp Tyr Ile Gly 
                245                 250                 255     


Gly Tyr Val Phe Gly Gly Gly Thr Gln Leu Thr Val Thr Gly Glu Ser 
            260                 265                 270         


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val 
        275                 280                 285             


Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala 
    290                 295                 300                 


Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
305                 310                 315                 320 


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
                325                 330                 335     


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
            340                 345                 350         


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
        355                 360                 365             


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
    370                 375                 380                 


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
385                 390                 395                 400 


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
                405                 410                 415     


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
            420                 425                 430         


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
        435                 440                 445             


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460                 


Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
465                 470                 475                 480 


Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu 
                485                 490                 495     


Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys 
            500                 505                 510         


Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile 
        515                 520                 525             


Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly 
    530                 535                 540                 


Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His 
545                 550                 555                 560 


Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys 
                565                 570                 575     


Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr 
            580                 585                 590         


Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys 
        595                 600                 605             


Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser 
    610                 615                 620                 


Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile 
625                 630                 635                 640 


Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys 
                645                 650                 655     


Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly 
            660                 665                 670         


Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser 
        675                 680                 685             


Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg 
    690                 695                 700                 


Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu 
705                 710                 715                 720 


Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His 
                725                 730                 735     


Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly 
            740                 745                 750         


Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys 
        755                 760                 765             


Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val 
    770                 775                 780                 


Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn 
785                 790                 795                 800 


Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn 
                805                 810                 815     


Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu 
            820                 825                 830         


Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
        835                 840                 845 


<210>  57
<211>  952
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader _R12- Hinge- CH3- CD28tm/41BB-Z-T2A-tEGFR

<400>  57

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Glu Gln Leu Val Glu Ser Gly Gly Arg 
            20                  25                  30          


Leu Val Thr Pro Gly Gly Ser Leu Thr Leu Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Phe Asp Phe Ser Ala Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Ile Ala Thr Ile Tyr Pro Ser Ser Gly Lys Thr 
65                  70                  75                  80  


Tyr Tyr Ala Thr Trp Val Asn Gly Arg Phe Thr Ile Ser Ser Asp Asn 
                85                  90                  95      


Ala Gln Asn Thr Val Asp Leu Gln Met Asn Ser Leu Thr Ala Ala Asp 
            100                 105                 110         


Arg Ala Thr Tyr Phe Cys Ala Arg Asp Ser Tyr Ala Asp Asp Gly Ala 
        115                 120                 125             


Leu Phe Asn Ile Trp Gly Pro Gly Thr Leu Val Thr Ile Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu 
145                 150                 155                 160 


Val Leu Thr Gln Ser Pro Ser Val Ser Ala Ala Leu Gly Ser Pro Ala 
                165                 170                 175     


Lys Ile Thr Cys Thr Leu Ser Ser Ala His Lys Thr Asp Thr Ile Asp 
            180                 185                 190         


Trp Tyr Gln Gln Leu Gln Gly Glu Ala Pro Arg Tyr Leu Met Gln Val 
        195                 200                 205             


Gln Ser Asp Gly Ser Tyr Thr Lys Arg Pro Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Ser Ser Gly Ala Asp Arg Tyr Leu Ile Ile Pro Ser Val 
225                 230                 235                 240 


Gln Ala Asp Asp Glu Ala Asp Tyr Tyr Cys Gly Ala Asp Tyr Ile Gly 
                245                 250                 255     


Gly Tyr Val Phe Gly Gly Gly Thr Gln Leu Thr Val Thr Gly Glu Ser 
            260                 265                 270         


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg Glu Pro 
        275                 280                 285             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    290                 295                 300                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
305                 310                 315                 320 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                325                 330                 335     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            340                 345                 350         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        355                 360                 365             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    370                 375                 380                 


Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val 
385                 390                 395                 400 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
                405                 410                 415     


Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
            420                 425                 430         


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
        435                 440                 445             


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
    450                 455                 460                 


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
465                 470                 475                 480 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                485                 490                 495     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
            500                 505                 510         


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
        515                 520                 525             


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
    530                 535                 540                 


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
545                 550                 555                 560 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly 
                565                 570                 575     


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
            580                 585                 590         


Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu 
        595                 600                 605             


Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile 
    610                 615                 620                 


Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile 
625                 630                 635                 640 


Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu 
                645                 650                 655     


Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp 
            660                 665                 670         


Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe 
        675                 680                 685             


Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe 
    690                 695                 700                 


Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe 
705                 710                 715                 720 


Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser 
                725                 730                 735     


Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn 
            740                 745                 750         


Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser 
        755                 760                 765             


Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys 
    770                 775                 780                 


Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp 
785                 790                 795                 800 


Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly 
                805                 810                 815     


Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu 
            820                 825                 830         


Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro 
        835                 840                 845             


Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile 
    850                 855                 860                 


Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro 
865                 870                 875                 880 


Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp 
                885                 890                 895     


Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys 
            900                 905                 910         


Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro 
        915                 920                 925             


Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val 
    930                 935                 940                 


Ala Leu Gly Ile Gly Leu Phe Met 
945                 950         


<210>  58
<211>  1062
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader _R12- Hinge-CH2-CH3- CD28tm/41BB-Z-T2A-tEGFR

<400>  58

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Glu Gln Leu Val Glu Ser Gly Gly Arg 
            20                  25                  30          


Leu Val Thr Pro Gly Gly Ser Leu Thr Leu Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Phe Asp Phe Ser Ala Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Ile Ala Thr Ile Tyr Pro Ser Ser Gly Lys Thr 
65                  70                  75                  80  


Tyr Tyr Ala Thr Trp Val Asn Gly Arg Phe Thr Ile Ser Ser Asp Asn 
                85                  90                  95      


Ala Gln Asn Thr Val Asp Leu Gln Met Asn Ser Leu Thr Ala Ala Asp 
            100                 105                 110         


Arg Ala Thr Tyr Phe Cys Ala Arg Asp Ser Tyr Ala Asp Asp Gly Ala 
        115                 120                 125             


Leu Phe Asn Ile Trp Gly Pro Gly Thr Leu Val Thr Ile Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu 
145                 150                 155                 160 


Val Leu Thr Gln Ser Pro Ser Val Ser Ala Ala Leu Gly Ser Pro Ala 
                165                 170                 175     


Lys Ile Thr Cys Thr Leu Ser Ser Ala His Lys Thr Asp Thr Ile Asp 
            180                 185                 190         


Trp Tyr Gln Gln Leu Gln Gly Glu Ala Pro Arg Tyr Leu Met Gln Val 
        195                 200                 205             


Gln Ser Asp Gly Ser Tyr Thr Lys Arg Pro Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Ser Ser Gly Ala Asp Arg Tyr Leu Ile Ile Pro Ser Val 
225                 230                 235                 240 


Gln Ala Asp Asp Glu Ala Asp Tyr Tyr Cys Gly Ala Asp Tyr Ile Gly 
                245                 250                 255     


Gly Tyr Val Phe Gly Gly Gly Thr Gln Leu Thr Val Thr Gly Glu Ser 
            260                 265                 270         


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly 
        275                 280                 285             


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
    290                 295                 300                 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
305                 310                 315                 320 


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
                325                 330                 335     


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr 
            340                 345                 350         


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
        355                 360                 365             


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
    370                 375                 380                 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
385                 390                 395                 400 


Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser 
                405                 410                 415     


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
            420                 425                 430         


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
        435                 440                 445             


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val 
    450                 455                 460                 


Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met 
465                 470                 475                 480 


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
                485                 490                 495     


Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            500                 505                 510         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys 
        515                 520                 525             


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
    530                 535                 540                 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
545                 550                 555                 560 


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
                565                 570                 575     


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
            580                 585                 590         


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
        595                 600                 605             


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
625                 630                 635                 640 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                645                 650                 655     


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            660                 665                 670         


Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly Gly Glu Gly 
        675                 680                 685             


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
    690                 695                 700                 


Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His 
705                 710                 715                 720 


Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile 
                725                 730                 735     


Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His 
            740                 745                 750         


Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val 
        755                 760                 765             


Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln 
    770                 775                 780                 


Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu 
785                 790                 795                 800 


Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn 
                805                 810                 815     


Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu 
            820                 825                 830         


Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys 
        835                 840                 845             


Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys 
    850                 855                 860                 


Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln 
865                 870                 875                 880 


Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr 
                885                 890                 895     


Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro 
            900                 905                 910         


Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu 
        915                 920                 925             


Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val 
    930                 935                 940                 


Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala 
945                 950                 955                 960 


Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys 
                965                 970                 975     


Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly 
            980                 985                 990         


Val Met Gly Glu Asn Asn Thr Leu  Val Trp Lys Tyr Ala  Asp Ala Gly 
        995                 1000                 1005             


His Val  Cys His Leu Cys His  Pro Asn Cys Thr Tyr  Gly Cys Thr 
    1010                 1015                 1020             


Gly Pro  Gly Leu Glu Gly Cys  Pro Thr Asn Gly Pro  Lys Ile Pro 
    1025                 1030                 1035             


Ser Ile  Ala Thr Gly Met Val  Gly Ala Leu Leu Leu  Leu Leu Val 
    1040                 1045                 1050             


Val Ala  Leu Gly Ile Gly Leu  Phe Met 
    1055                 1060         


<210>  59
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leader Sequence

<400>  59
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacaccca                    48


<210>  60
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker Peptide

<400>  60

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  61
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Long Spacer

<400>  61

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  62
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Long Spacer

<400>  62
gagagcaagt acggaccgcc ctgcccccct tgccctgccc ccgagttcct gggcggaccc       60

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagccg gacccccgag      120

gtgacctgcg tggtggtgga cgtgagccag gaagatcccg aggtccagtt caattggtac      180

gtggacggcg tggaagtgca caacgccaag accaagccca gagaggaaca gttcaacagc      240

acctaccggg tggtgtctgt gctgaccgtg ctgcaccagg actggctgaa cggcaaagaa      300

tacaagtgca aggtgtccaa caagggcctg cccagcagca tcgaaaagac catcagcaag      360

gccaagggcc agcctcgcga gccccaggtg tacaccctgc ctccctccca ggaagagatg      420

accaagaacc aggtgtccct gacctgcctg gtgaagggct tctaccccag cgacatcgcc      480

gtggagtggg agagcaacgg ccagcctgag aacaactaca agaccacccc tcccgtgctg      540

gacagcgacg gcagcttctt cctgtacagc cggctgaccg tggacaagag ccggtggcag      600

gaaggcaacg tctttagctg cagcgtgatg cacgaggccc tgcacaacca ctacacccag      660

aagagcctga gcctgtccct gggcaag                                          687


<210>  63
<211>  622
<212>  PRT
<213>  Homo sapiens

<400>  63

Met Ala Leu Pro Thr Ala Arg Pro Leu Leu Gly Ser Cys Gly Thr Pro 
1               5                   10                  15      


Ala Leu Gly Ser Leu Leu Phe Leu Leu Phe Ser Leu Gly Trp Val Gln 
            20                  25                  30          


Pro Ser Arg Thr Leu Ala Gly Glu Thr Gly Gln Glu Ala Ala Pro Leu 
        35                  40                  45              


Asp Gly Val Leu Ala Asn Pro Pro Asn Ile Ser Ser Leu Ser Pro Arg 
    50                  55                  60                  


Gln Leu Leu Gly Phe Pro Cys Ala Glu Val Ser Gly Leu Ser Thr Glu 
65                  70                  75                  80  


Arg Val Arg Glu Leu Ala Val Ala Leu Ala Gln Lys Asn Val Lys Leu 
                85                  90                  95      


Ser Thr Glu Gln Leu Arg Cys Leu Ala His Arg Leu Ser Glu Pro Pro 
            100                 105                 110         


Glu Asp Leu Asp Ala Leu Pro Leu Asp Leu Leu Leu Phe Leu Asn Pro 
        115                 120                 125             


Asp Ala Phe Ser Gly Pro Gln Ala Cys Thr His Phe Phe Ser Arg Ile 
    130                 135                 140                 


Thr Lys Ala Asn Val Asp Leu Leu Pro Arg Gly Ala Pro Glu Arg Gln 
145                 150                 155                 160 


Arg Leu Leu Pro Ala Ala Leu Ala Cys Trp Gly Val Arg Gly Ser Leu 
                165                 170                 175     


Leu Ser Glu Ala Asp Val Arg Ala Leu Gly Gly Leu Ala Cys Asp Leu 
            180                 185                 190         


Pro Gly Arg Phe Val Ala Glu Ser Ala Glu Val Leu Leu Pro Arg Leu 
        195                 200                 205             


Val Ser Cys Pro Gly Pro Leu Asp Gln Asp Gln Gln Glu Ala Ala Arg 
    210                 215                 220                 


Ala Ala Leu Gln Gly Gly Gly Pro Pro Tyr Gly Pro Pro Ser Thr Trp 
225                 230                 235                 240 


Ser Val Ser Thr Met Asp Ala Leu Arg Gly Leu Leu Pro Val Leu Gly 
                245                 250                 255     


Gln Pro Ile Ile Arg Ser Ile Pro Gln Gly Ile Val Ala Ala Trp Arg 
            260                 265                 270         


Gln Arg Ser Ser Arg Asp Pro Ser Trp Arg Gln Pro Glu Arg Thr Ile 
        275                 280                 285             


Leu Arg Pro Arg Phe Arg Arg Glu Val Glu Lys Thr Ala Cys Pro Ser 
    290                 295                 300                 


Gly Lys Lys Ala Arg Glu Ile Asp Glu Ser Leu Ile Phe Tyr Lys Lys 
305                 310                 315                 320 


Trp Glu Leu Glu Ala Cys Val Asp Ala Ala Leu Leu Ala Thr Gln Met 
                325                 330                 335     


Asp Arg Val Asn Ala Ile Pro Phe Thr Tyr Glu Gln Leu Asp Val Leu 
            340                 345                 350         


Lys His Lys Leu Asp Glu Leu Tyr Pro Gln Gly Tyr Pro Glu Ser Val 
        355                 360                 365             


Ile Gln His Leu Gly Tyr Leu Phe Leu Lys Met Ser Pro Glu Asp Ile 
    370                 375                 380                 


Arg Lys Trp Asn Val Thr Ser Leu Glu Thr Leu Lys Ala Leu Leu Glu 
385                 390                 395                 400 


Val Asn Lys Gly His Glu Met Ser Pro Gln Val Ala Thr Leu Ile Asp 
                405                 410                 415     


Arg Phe Val Lys Gly Arg Gly Gln Leu Asp Lys Asp Thr Leu Asp Thr 
            420                 425                 430         


Leu Thr Ala Phe Tyr Pro Gly Tyr Leu Cys Ser Leu Ser Pro Glu Glu 
        435                 440                 445             


Leu Ser Ser Val Pro Pro Ser Ser Ile Trp Ala Val Arg Pro Gln Asp 
    450                 455                 460                 


Leu Asp Thr Cys Asp Pro Arg Gln Leu Asp Val Leu Tyr Pro Lys Ala 
465                 470                 475                 480 


Arg Leu Ala Phe Gln Asn Met Asn Gly Ser Glu Tyr Phe Val Lys Ile 
                485                 490                 495     


Gln Ser Phe Leu Gly Gly Ala Pro Thr Glu Asp Leu Lys Ala Leu Ser 
            500                 505                 510         


Gln Gln Asn Val Ser Met Asp Leu Ala Thr Phe Met Lys Leu Arg Thr 
        515                 520                 525             


Asp Ala Val Leu Pro Leu Thr Val Ala Glu Val Gln Lys Leu Leu Gly 
    530                 535                 540                 


Pro His Val Glu Gly Leu Lys Ala Glu Glu Arg His Arg Pro Val Arg 
545                 550                 555                 560 


Asp Trp Ile Leu Arg Gln Arg Gln Asp Asp Leu Asp Thr Leu Gly Leu 
                565                 570                 575     


Gly Leu Gln Gly Gly Ile Pro Asn Gly Tyr Leu Val Leu Asp Leu Ser 
            580                 585                 590         


Val Gln Glu Ala Leu Ser Gly Thr Pro Cys Leu Leu Gly Pro Gly Pro 
        595                 600                 605             


Val Leu Thr Val Leu Ala Leu Leu Leu Ala Ser Thr Leu Ala 
    610                 615                 620         


<210>  64
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mid-Ampr primer

<400>  64
ttgagagttt tcgccccg                                                     18


<210>  65
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified Human IgG4

<400>  65

Glu Val Val Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10              


<210>  66
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified Human IgG4

<400>  66

Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10  


<210>  67
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified Human IgG4

<400>  67

Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   


<210>  68
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified Human IgG4

<400>  68

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  69
<211>  750
<212>  PRT
<213>  Homo sapiens

<400>  69

Met Trp Asn Leu Leu His Glu Thr Asp Ser Ala Val Ala Thr Ala Arg 
1               5                   10                  15      


Arg Pro Arg Trp Leu Cys Ala Gly Ala Leu Val Leu Ala Gly Gly Phe 
            20                  25                  30          


Phe Leu Leu Gly Phe Leu Phe Gly Trp Phe Ile Lys Ser Ser Asn Glu 
        35                  40                  45              


Ala Thr Asn Ile Thr Pro Lys His Asn Met Lys Ala Phe Leu Asp Glu 
    50                  55                  60                  


Leu Lys Ala Glu Asn Ile Lys Lys Phe Leu Tyr Asn Phe Thr Gln Ile 
65                  70                  75                  80  


Pro His Leu Ala Gly Thr Glu Gln Asn Phe Gln Leu Ala Lys Gln Ile 
                85                  90                  95      


Gln Ser Gln Trp Lys Glu Phe Gly Leu Asp Ser Val Glu Leu Ala His 
            100                 105                 110         


Tyr Asp Val Leu Leu Ser Tyr Pro Asn Lys Thr His Pro Asn Tyr Ile 
        115                 120                 125             


Ser Ile Ile Asn Glu Asp Gly Asn Glu Ile Phe Asn Thr Ser Leu Phe 
    130                 135                 140                 


Glu Pro Pro Pro Pro Gly Tyr Glu Asn Val Ser Asp Ile Val Pro Pro 
145                 150                 155                 160 


Phe Ser Ala Phe Ser Pro Gln Gly Met Pro Glu Gly Asp Leu Val Tyr 
                165                 170                 175     


Val Asn Tyr Ala Arg Thr Glu Asp Phe Phe Lys Leu Glu Arg Asp Met 
            180                 185                 190         


Lys Ile Asn Cys Ser Gly Lys Ile Val Ile Ala Arg Tyr Gly Lys Val 
        195                 200                 205             


Phe Arg Gly Asn Lys Val Lys Asn Ala Gln Leu Ala Gly Ala Lys Gly 
    210                 215                 220                 


Val Ile Leu Tyr Ser Asp Pro Ala Asp Tyr Phe Ala Pro Gly Val Lys 
225                 230                 235                 240 


Ser Tyr Pro Asp Gly Trp Asn Leu Pro Gly Gly Gly Val Gln Arg Gly 
                245                 250                 255     


Asn Ile Leu Asn Leu Asn Gly Ala Gly Asp Pro Leu Thr Pro Gly Tyr 
            260                 265                 270         


Pro Ala Asn Glu Tyr Ala Tyr Arg Arg Gly Ile Ala Glu Ala Val Gly 
        275                 280                 285             


Leu Pro Ser Ile Pro Val His Pro Ile Gly Tyr Tyr Asp Ala Gln Lys 
    290                 295                 300                 


Leu Leu Glu Lys Met Gly Gly Ser Ala Pro Pro Asp Ser Ser Trp Arg 
305                 310                 315                 320 


Gly Ser Leu Lys Val Pro Tyr Asn Val Gly Pro Gly Phe Thr Gly Asn 
                325                 330                 335     


Phe Ser Thr Gln Lys Val Lys Met His Ile His Ser Thr Asn Glu Val 
            340                 345                 350         


Thr Arg Ile Tyr Asn Val Ile Gly Thr Leu Arg Gly Ala Val Glu Pro 
        355                 360                 365             


Asp Arg Tyr Val Ile Leu Gly Gly His Arg Asp Ser Trp Val Phe Gly 
    370                 375                 380                 


Gly Ile Asp Pro Gln Ser Gly Ala Ala Val Val His Glu Ile Val Arg 
385                 390                 395                 400 


Ser Phe Gly Thr Leu Lys Lys Glu Gly Trp Arg Pro Arg Arg Thr Ile 
                405                 410                 415     


Leu Phe Ala Ser Trp Asp Ala Glu Glu Phe Gly Leu Leu Gly Ser Thr 
            420                 425                 430         


Glu Trp Ala Glu Glu Asn Ser Arg Leu Leu Gln Glu Arg Gly Val Ala 
        435                 440                 445             


Tyr Ile Asn Ala Asp Ser Ser Ile Glu Gly Asn Tyr Thr Leu Arg Val 
    450                 455                 460                 


Asp Cys Thr Pro Leu Met Tyr Ser Leu Val His Asn Leu Thr Lys Glu 
465                 470                 475                 480 


Leu Lys Ser Pro Asp Glu Gly Phe Glu Gly Lys Ser Leu Tyr Glu Ser 
                485                 490                 495     


Trp Thr Lys Lys Ser Pro Ser Pro Glu Phe Ser Gly Met Pro Arg Ile 
            500                 505                 510         


Ser Lys Leu Gly Ser Gly Asn Asp Phe Glu Val Phe Phe Gln Arg Leu 
        515                 520                 525             


Gly Ile Ala Ser Gly Arg Ala Arg Tyr Thr Lys Asn Trp Glu Thr Asn 
    530                 535                 540                 


Lys Phe Ser Gly Tyr Pro Leu Tyr His Ser Val Tyr Glu Thr Tyr Glu 
545                 550                 555                 560 


Leu Val Glu Lys Phe Tyr Asp Pro Met Phe Lys Tyr His Leu Thr Val 
                565                 570                 575     


Ala Gln Val Arg Gly Gly Met Val Phe Glu Leu Ala Asn Ser Ile Val 
            580                 585                 590         


Leu Pro Phe Asp Cys Arg Asp Tyr Ala Val Val Leu Arg Lys Tyr Ala 
        595                 600                 605             


Asp Lys Ile Tyr Ser Ile Ser Met Lys His Pro Gln Glu Met Lys Thr 
    610                 615                 620                 


Tyr Ser Val Ser Phe Asp Ser Leu Phe Ser Ala Val Lys Asn Phe Thr 
625                 630                 635                 640 


Glu Ile Ala Ser Lys Phe Ser Glu Arg Leu Gln Asp Phe Asp Lys Ser 
                645                 650                 655     


Asn Pro Ile Val Leu Arg Met Met Asn Asp Gln Leu Met Phe Leu Glu 
            660                 665                 670         


Arg Ala Phe Ile Asp Pro Leu Gly Leu Pro Asp Arg Pro Phe Tyr Arg 
        675                 680                 685             


His Val Ile Tyr Ala Pro Ser Ser His Asn Lys Tyr Ala Gly Glu Ser 
    690                 695                 700                 


Phe Pro Gly Ile Tyr Asp Ala Leu Phe Asp Ile Glu Ser Lys Val Asp 
705                 710                 715                 720 


Pro Ser Lys Ala Trp Gly Glu Val Lys Arg Gln Ile Tyr Val Ala Ala 
                725                 730                 735     


Phe Thr Val Gln Ala Ala Ala Glu Thr Leu Ser Glu Val Ala 
            740                 745                 750 


<210>  70
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  post-Ampr primer

<400>  70
aatagacaga tcgctgagat aggt                                              24


<210>  71
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pre-U5 primer

<400>  71
atcaaaagaa tagaccgaga tagggt                                            26


<210>  72
<211>  123
<212>  PRT
<213>  Homo sapiens

<400>  72

Met Lys Ala Val Leu Leu Ala Leu Leu Met Ala Gly Leu Ala Leu Gln 
1               5                   10                  15      


Pro Gly Thr Ala Leu Leu Cys Tyr Ser Cys Lys Ala Gln Val Ser Asn 
            20                  25                  30          


Glu Asp Cys Leu Gln Val Glu Asn Cys Thr Gln Leu Gly Glu Gln Cys 
        35                  40                  45              


Trp Thr Ala Arg Ile Arg Ala Val Gly Leu Leu Thr Val Ile Ser Lys 
    50                  55                  60                  


Gly Cys Ser Leu Asn Cys Val Asp Asp Ser Gln Asp Tyr Tyr Val Gly 
65                  70                  75                  80  


Lys Lys Asn Ile Thr Cys Cys Asp Thr Asp Leu Cys Asn Ala Ser Gly 
                85                  90                  95      


Ala His Ala Leu Gln Pro Ala Ala Ala Ile Leu Ala Leu Leu Pro Ala 
            100                 105                 110         


Leu Gly Leu Leu Leu Trp Gly Pro Gly Gln Leu 
        115                 120             


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  psi primer

<400>  73
gcagggagct agaacgattc                                                   20


<210>  74
<211>  10014
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R11 intermediate spacer CAR: PJ_R11-CH3-41BB-Z-T2A-tEGFR

<400>  74
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagcgaattc gccaccatgc tgctgctggt     2100

gacaagcctg ctgctgtgcg agctgcccca ccccgccttt ctgctgatcc cccagagcgt     2160

gaaagagtcc gagggcgacc tggtcacacc agccggcaac ctgaccctga cctgtaccgc     2220

cagcggcagc gacatcaacg actaccccat ctcttgggtc cgccaggctc ctggcaaggg     2280

actggaatgg atcggcttca tcaacagcgg cggcagcact tggtacgcca gctgggtcaa     2340

aggccggttc accatcagcc ggaccagcac caccgtggac ctgaagatga caagcctgac     2400

caccgacgac accgccacct acttttgcgc cagaggctac agcacctact acggcgactt     2460

caacatctgg ggccctggca ccctggtcac aatctctagc ggcggaggcg gcagcggagg     2520

tggaggaagt ggcggcggag gatccgagct ggtcatgacc cagaccccca gcagcacatc     2580

tggcgccgtg ggcggcaccg tgaccatcaa ttgccaggcc agccagagca tcgacagcaa     2640

cctggcctgg ttccagcaga agcccggcca gccccccacc ctgctgatct acagagcctc     2700

caacctggcc agcggcgtgc caagcagatt cagcggcagc agatctggca ccgagtacac     2760

cctgaccatc tccggcgtgc agagagagga cgccgctacc tattactgcc tgggcggcgt     2820

gggcaacgtg tcctacagaa ccagcttcgg cggaggtact gaggtggtcg tcaaatagga     2880

ccgccctgcc ccccttgccc tgcccccgag ttcctgggcg gacccagcgt gttcctgttc     2940

ccccccaagc ccaaggacac cctgatgatc agccggaccc ccgaggtgac ctgcgtggtg     3000

gtggacgtga gccaggaaga tcccgaggtc cagttcaatt ggtacgtgga cggcgtggaa     3060

gtgcacaacg ccaagaccaa gcccagagag gaacagttca acagcaccta ccgggtggtg     3120

tctgtgctga ccgtgctgca ccaggactgg ctgaacggca aagaatacaa gtgcaaggtg     3180

tccaacaagg gcctgcccag cagcatcgaa aagaccatca gcaaggccaa gggccagcct     3240

cgcgagcccc aggtgtacac cctgcctccc tcccaggaag agatgaccaa gaaccaggtg     3300

tccctgacct gcctggtgaa gggcttctac cccagcgaca tcgccgtgga gtgggagagc     3360

aacggccagc ctgagaacaa ctacaagacc acccctcccg tgctggacag cgacggcagc     3420

ttcttcctgt acagccggct gaccgtggac aagagccggt ggcaggaagg caacgtcttt     3480

agctgcagcg tgatgcacga ggccctgcac aaccactaca cccagaagag cctgagcctg     3540

tccctgggca agatgttctg ggtgctggtg gtggtgggcg gggtgctggc ctgctacagc     3600

ctgctggtga cagtggcctt catcatcttt tgggtgaaac ggggcagaaa gaaactcctg     3660

tatatattca aacaaccatt tatgagacca gtacaaacta ctcaagagga agatggctgt     3720

agctgccgat ttccagaaga agaagaagga ggatgtgaac tgcgggtgaa gttcagcaga     3780

agcgccgacg cccctgccta ccagcagggc cagaatcagc tgtacaacga gctgaacctg     3840

ggcagaaggg aagagtacga cgtcctggat aagcggagag gccgggaccc tgagatgggc     3900

ggcaagcctc ggcggaagaa cccccaggaa ggcctgtata acgaactgca gaaagacaag     3960

atggccgagg cctacagcga gatcggcatg aagggcgagc ggaggcgggg caagggccac     4020

gacggcctgt atcagggcct gtccaccgcc accaaggata cctacgacgc cctgcacatg     4080

caggccctgc ccccaaggct cgagggcggc ggagagggca gaggaagtct tctaacatgc     4140

ggtgacgtgg aggagaatcc cggccctagg atgcttctcc tggtgacaag ccttctgctc     4200

tgtgagttac cacacccagc attcctcctg atcccacgca aagtgtgtaa cggaataggt     4260

attggtgaat ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac     4320

tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc     4380

acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc     4440

acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag     4500

aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc     4560

agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg     4620

ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt     4680

gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc     4740

acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg     4800

gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt     4860

ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag     4920

tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag     4980

tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga     5040

gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat     5100

ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct     5160

aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc     5220

ctggggatcg gcctcttcat gtgagcggcc gctctagacc cgggctgcag gaattcgata     5280

tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc     5340

ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg     5400

ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc     5460

tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg     5520

acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg     5580

ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga     5640

caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct     5700

ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg     5760

tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc     5820

ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc     5880

cgcatcgata ccgtcgacta gccgtacctt taagaccaat gacttacaag gcagctgtag     5940

atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa     6000

gacaagatct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg     6060

agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc     6120

ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct     6180

tttagtcagt gtggaaaatc tctagcagaa ttcgatatca agcttatcga taccgtcgac     6240

ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattaca attcactggc     6300

cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc     6360

agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc     6420

ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa     6480

ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa     6540

atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac     6600

aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag     6660

ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt     6720

aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg     6780

gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca     6840

agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag     6900

ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc     6960

taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa     7020

tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt     7080

gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct     7140

gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc     7200

cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta     7260

tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac     7320

tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc     7380

atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac     7440

ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg     7500

gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac     7560

gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc     7620

gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt     7680

gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga     7740

gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc     7800

cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag     7860

atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca     7920

tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc     7980

ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca     8040

gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc     8100

tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta     8160

ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt     8220

ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc     8280

gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg     8340

ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg     8400

tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag     8460

ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc     8520

agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat     8580

agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg     8640

gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc     8700

tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt     8760

accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca     8820

gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg     8880

attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac     8940

gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg     9000

gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac     9060

catgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac     9120

cgcggtggcg gcctcgaggt cgagatccgg tcgaccagca accatagtcc cgcccctaac     9180

tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact     9240

aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta     9300

gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttcgacggt atcgattggc     9360

tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca     9420

attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta     9480

aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat     9540

gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg     9600

taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac     9660

gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt     9720

cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg     9780

cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc     9840

attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt     9900

aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggaattc ggagtggcga     9960

gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctg          10014


<210>  75
<211>  10015
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R11 long spacer CAR: PJ_R11-CH2-CH3-41BB-Z-T2A-tEGFR

<400>  75
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagcgaattc gccaccatgc tgctgctggt     2100

gacaagcctg ctgctgtgcg agctgcccca ccccgccttt ctgctgatcc cccagagcgt     2160

gaaagagtcc gagggcgacc tggtcacacc agccggcaac ctgaccctga cctgtaccgc     2220

cagcggcagc gacatcaacg actaccccat ctcttgggtc cgccaggctc ctggcaaggg     2280

actggaatgg atcggcttca tcaacagcgg cggcagcact tggtacgcca gctgggtcaa     2340

aggccggttc accatcagcc ggaccagcac caccgtggac ctgaagatga caagcctgac     2400

caccgacgac accgccacct acttttgcgc cagaggctac agcacctact acggcgactt     2460

caacatctgg ggccctggca ccctggtcac aatctctagc ggcggaggcg gcagcggagg     2520

tggaggaagt ggcggcggag gatccgagct ggtcatgacc cagaccccca gcagcacatc     2580

tggcgccgtg ggcggcaccg tgaccatcaa ttgccaggcc agccagagca tcgacagcaa     2640

cctggcctgg ttccagcaga agcccggcca gccccccacc ctgctgatct acagagcctc     2700

caacctggcc agcggcgtgc caagcagatt cagcggcagc agatctggca ccgagtacac     2760

cctgaccatc tccggcgtgc agagagagga cgccgctacc tattactgcc tgggcggcgt     2820

gggcaacgtg tcctacagaa ccagcttcgg cggaggtact gaggtggtcg tcaaatacgg     2880

accgccctgc cccccttgcc ctgcccccga gttcctgggc ggacccagcg tgttcctgtt     2940

cccccccaag cccaaggaca ccctgatgat cagccggacc cccgaggtga cctgcgtggt     3000

ggtggacgtg agccaggaag atcccgaggt ccagttcaat tggtacgtgg acggcgtgga     3060

agtgcacaac gccaagacca agcccagaga ggaacagttc aacagcacct accgggtggt     3120

gtctgtgctg accgtgctgc accaggactg gctgaacggc aaagaataca agtgcaaggt     3180

gtccaacaag ggcctgccca gcagcatcga aaagaccatc agcaaggcca agggccagcc     3240

tcgcgagccc caggtgtaca ccctgcctcc ctcccaggaa gagatgacca agaaccaggt     3300

gtccctgacc tgcctggtga agggcttcta ccccagcgac atcgccgtgg agtgggagag     3360

caacggccag cctgagaaca actacaagac cacccctccc gtgctggaca gcgacggcag     3420

cttcttcctg tacagccggc tgaccgtgga caagagccgg tggcaggaag gcaacgtctt     3480

tagctgcagc gtgatgcacg aggccctgca caaccactac acccagaaga gcctgagcct     3540

gtccctgggc aagatgttct gggtgctggt ggtggtgggc ggggtgctgg cctgctacag     3600

cctgctggtg acagtggcct tcatcatctt ttgggtgaaa cggggcagaa agaaactcct     3660

gtatatattc aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg     3720

tagctgccga tttccagaag aagaagaagg aggatgtgaa ctgcgggtga agttcagcag     3780

aagcgccgac gcccctgcct accagcaggg ccagaatcag ctgtacaacg agctgaacct     3840

gggcagaagg gaagagtacg acgtcctgga taagcggaga ggccgggacc ctgagatggg     3900

cggcaagcct cggcggaaga acccccagga aggcctgtat aacgaactgc agaaagacaa     3960

gatggccgag gcctacagcg agatcggcat gaagggcgag cggaggcggg gcaagggcca     4020

cgacggcctg tatcagggcc tgtccaccgc caccaaggat acctacgacg ccctgcacat     4080

gcaggccctg cccccaaggc tcgagggcgg cggagagggc agaggaagtc ttctaacatg     4140

cggtgacgtg gaggagaatc ccggccctag gatgcttctc ctggtgacaa gccttctgct     4200

ctgtgagtta ccacacccag cattcctcct gatcccacgc aaagtgtgta acggaatagg     4260

tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa     4320

ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt     4380

cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat     4440

cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga     4500

gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt     4560

cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt     4620

gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt     4680

tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc     4740

cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag     4800

ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct     4860

tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga     4920

gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca     4980

gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg     5040

agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca     5100

tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc     5160

taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc     5220

cctggggatc ggcctcttca tgtgagcggc cgctctagac ccgggctgca ggaattcgat     5280

atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt     5340

cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat     5400

gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct     5460

ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct     5520

gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc     5580

gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg     5640

acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc     5700

tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac     5760

gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg     5820

cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc     5880

ccgcatcgat accgtcgact agccgtacct ttaagaccaa tgacttacaa ggcagctgta     5940

gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga     6000

agacaagatc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg     6060

gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg     6120

cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc     6180

ttttagtcag tgtggaaaat ctctagcaga attcgatatc aagcttatcg ataccgtcga     6240

cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac aattcactgg     6300

ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg     6360

cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt     6420

cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa     6480

attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa     6540

aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa     6600

caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca     6660

gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg     6720

taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc     6780

ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc     6840

aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca     6900

gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt     6960

ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata     7020

atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt     7080

tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc     7140

tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat     7200

ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct     7260

atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca     7320

ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg     7380

catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa     7440

cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg     7500

ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga     7560

cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg     7620

cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt     7680

tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg     7740

agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc     7800

ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca     7860

gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc     7920

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     7980

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     8040

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg     8100

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct     8160

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct     8220

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct     8280

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg     8340

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc     8400

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga     8460

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg     8520

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta     8580

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg     8640

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg     8700

ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat     8760

taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc     8820

agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc     8880

gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa     8940

cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc     9000

ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga     9060

ccatgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggagctcca     9120

ccgcggtggc ggcctcgagg tcgagatccg gtcgaccagc aaccatagtc ccgcccctaa     9180

ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac     9240

taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt     9300

agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttcgacgg tatcgattgg     9360

ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc     9420

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt     9480

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta     9540

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg     9600

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga     9660

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt     9720

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg     9780

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc     9840

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg     9900

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggaatt cggagtggcg     9960

agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctg         10015


<210>  76
<211>  241
<212>  PRT
<213>  Homo sapiens

<400>  76

Gln Ser Val Lys Glu Ser Glu Gly Asp Leu Val Thr Pro Ala Gly Asn 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Ala Ser Gly Ser Asp Ile Asn Asp Tyr Pro 
            20                  25                  30          


Ile Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Phe Ile Asn Ser Gly Gly Ser Thr Trp Tyr Ala Ser Trp Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Thr Ser Thr Thr Val Asp Leu Lys Met Thr 
65                  70                  75                  80  


Ser Leu Thr Thr Asp Asp Thr Ala Thr Tyr Phe Cys Ala Arg Gly Tyr 
                85                  90                  95      


Ser Thr Tyr Tyr Gly Asp Phe Asn Ile Trp Gly Pro Gly Thr Leu Val 
            100                 105                 110         


Thr Ile Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Glu Leu Val Met Thr Gln Thr Pro Ser Ser Thr Ser Gly 
    130                 135                 140                 


Ala Val Gly Gly Thr Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Ile 
145                 150                 155                 160 


Asp Ser Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Thr 
                165                 170                 175     


Leu Leu Ile Tyr Arg Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg 
            180                 185                 190         


Phe Ser Gly Ser Arg Ser Gly Thr Glu Tyr Thr Leu Thr Ile Ser Gly 
        195                 200                 205             


Val Gln Arg Glu Asp Ala Ala Thr Tyr Tyr Cys Leu Gly Gly Val Gly 
    210                 215                 220                 


Asn Val Ser Tyr Arg Thr Ser Phe Gly Gly Gly Thr Glu Val Val Val 
225                 230                 235                 240 


Lys 
    


<210>  77
<211>  801
<212>  DNA
<213>  Homo sapiens

<400>  77
gaattcgcca ccatgctgct gctggtgaca agcctgctgc tgtgcgagct gccccacccc       60

gcctttctgc tgatccccca gagcgtgaaa gagtccgagg gcgacctggt cacaccagcc      120

ggcaacctga ccctgacctg taccgccagc ggcagcgaca tcaacgacta ccccatctct      180

tgggtccgcc aggctcctgg caagggactg gaatggatcg gcttcatcaa cagcggcggc      240

agcacttggt acgccagctg ggtcaaaggc cggttcacca tcagccggac cagcaccacc      300

gtggacctga agatgacaag cctgaccacc gacgacaccg ccacctactt ttgcgccaga      360

ggctacagca cctactacgg cgacttcaac atctggggcc ctggcaccct ggtcacaatc      420

tctagcggcg gaggcggcag cggaggtgga ggaagtggcg gcggaggatc cgagctggtc      480

atgacccaga cccccagcag cacatctggc gccgtgggcg gcaccgtgac catcaattgc      540

caggccagcc agagcatcga cagcaacctg gcctggttcc agcagaagcc cggccagccc      600

cccaccctgc tgatctacag agcctccaac ctggccagcg gcgtgccaag cagattcagc      660

ggcagcagat ctggcaccga gtacaccctg accatctccg gcgtgcagag agaggacgcc      720

gctacctatt actgcctggg cggcgtgggc aacgtgtcct acagaaccag cttcggcgga      780

ggtactgagg tggtcgtcaa a                                                801


<210>  78
<211>  9685
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R11 short spacer CAR: PJ_R11- 41BB-Z-T2A-tEGFR

<400>  78
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagcgaattc gccaccatgc tgctgctggt     2100

gacaagcctg ctgctgtgcg agctgcccca ccccgccttt ctgctgatcc cccagagcgt     2160

gaaagagtcc gagggcgacc tggtcacacc agccggcaac ctgaccctga cctgtaccgc     2220

cagcggcagc gacatcaacg actaccccat ctcttgggtc cgccaggctc ctggcaaggg     2280

actggaatgg atcggcttca tcaacagcgg cggcagcact tggtacgcca gctgggtcaa     2340

aggccggttc accatcagcc ggaccagcac caccgtggac ctgaagatga caagcctgac     2400

caccgacgac accgccacct acttttgcgc cagaggctac agcacctact acggcgactt     2460

caacatctgg ggccctggca ccctggtcac aatctctagc ggcggaggcg gcagcggagg     2520

tggaggaagt ggcggcggag gatccgagct ggtcatgacc cagaccccca gcagcacatc     2580

tggcgccgtg ggcggcaccg tgaccatcaa ttgccaggcc agccagagca tcgacagcaa     2640

cctggcctgg ttccagcaga agcccggcca gccccccacc ctgctgatct acagagcctc     2700

caacctggcc agcggcgtgc caagcagatt cagcggcagc agatctggca ccgagtacac     2760

cctgaccatc tccggcgtgc agagagagga cgccgctacc tattactgcc tgggcggcgt     2820

gggcaacgtg tcctacagaa ccagcttcgg cggaggtact gaggtggtcg tcaaatacgg     2880

accgccctgc cccccttgcc ctggccagcc tcgcgagccc caggtgtaca ccctgcctcc     2940

ctcccaggaa gagatgacca agaaccaggt gtccctgacc tgcctggtga agggcttcta     3000

ccccagcgac atcgccgtgg agtgggagag caacggccag cctgagaaca actacaagac     3060

cacccctccc gtgctggaca gcgacggcag cttcttcctg tacagccggc tgaccgtgga     3120

caagagccgg tggcaggaag gcaacgtctt tagctgcagc gtgatgcacg aggccctgca     3180

caaccactac acccagaaga gcctgagcct gtccctgggc aagatgttct gggtgctggt     3240

ggtggtgggc ggggtgctgg cctgctacag cctgctggtg acagtggcct tcatcatctt     3300

ttgggtgaaa cggggcagaa agaaactcct gtatatattc aaacaaccat ttatgagacc     3360

agtacaaact actcaagagg aagatggctg tagctgccga tttccagaag aagaagaagg     3420

aggatgtgaa ctgcgggtga agttcagcag aagcgccgac gcccctgcct accagcaggg     3480

ccagaatcag ctgtacaacg agctgaacct gggcagaagg gaagagtacg acgtcctgga     3540

taagcggaga ggccgggacc ctgagatggg cggcaagcct cggcggaaga acccccagga     3600

aggcctgtat aacgaactgc agaaagacaa gatggccgag gcctacagcg agatcggcat     3660

gaagggcgag cggaggcggg gcaagggcca cgacggcctg tatcagggcc tgtccaccgc     3720

caccaaggat acctacgacg ccctgcacat gcaggccctg cccccaaggc tcgagggcgg     3780

cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag     3840

gatgcttctc ctggtgacaa gccttctgct ctgtgagtta ccacacccag cattcctcct     3900

gatcccacgc aaagtgtgta acggaatagg tattggtgaa tttaaagact cactctccat     3960

aaatgctacg aatattaaac acttcaaaaa ctgcacctcc atcagtggcg atctccacat     4020

cctgccggtg gcatttaggg gtgactcctt cacacatact cctcctctgg atccacagga     4080

actggatatt ctgaaaaccg taaaggaaat cacagggttt ttgctgattc aggcttggcc     4140

tgaaaacagg acggacctcc atgcctttga gaacctagaa atcatacgcg gcaggaccaa     4200

gcaacatggt cagttttctc ttgcagtcgt cagcctgaac ataacatcct tgggattacg     4260

ctccctcaag gagataagtg atggagatgt gataatttca ggaaacaaaa atttgtgcta     4320

tgcaaataca ataaactgga aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat     4380

aagcaacaga ggtgaaaaca gctgcaaggc cacaggccag gtctgccatg ccttgtgctc     4440

ccccgagggc tgctggggcc cggagcccag ggactgcgtc tcttgccgga atgtcagccg     4500

aggcagggaa tgcgtggaca agtgcaacct tctggagggt gagccaaggg agtttgtgga     4560

gaactctgag tgcatacagt gccacccaga gtgcctgcct caggccatga acatcacctg     4620

cacaggacgg ggaccagaca actgtatcca gtgtgcccac tacattgacg gcccccactg     4680

cgtcaagacc tgcccggcag gagtcatggg agaaaacaac accctggtct ggaagtacgc     4740

agacgccggc catgtgtgcc acctgtgcca tccaaactgc acctacggat gcactgggcc     4800

aggtcttgaa ggctgtccaa cgaatgggcc taagatcccg tccatcgcca ctgggatggt     4860

gggggccctc ctcttgctgc tggtggtggc cctggggatc ggcctcttca tgtgagcggc     4920

cgctctagac ccgggctgca ggaattcgat atcaagctta tcgataatca acctctggat     4980

tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt     5040

ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc     5100

tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg     5160

caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc     5220

accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa     5280

ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat     5340

tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc     5400

tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt     5460

ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag     5520

acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgact agccgtacct     5580

ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg     5640

ggactggaag ggctaattca ctcccaaaga agacaagatc tgctttttgc ctgtactggg     5700

tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg     5760

cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt     5820

gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcaga     5880

attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc     5940

cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa     6000

accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta     6060

atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat     6120

ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc     6180

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga     6240

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc     6300

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc     6360

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag     6420

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa     6480

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac     6540

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa     6600

tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat     6660

gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca     6720

acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca     6780

cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta     6840

catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt     6900

tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc     6960

cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc     7020

accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc     7080

cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa     7140

ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga     7200

accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat     7260

ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca     7320

attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc     7380

ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat     7440

tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag     7500

tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa     7560

gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca     7620

tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc     7680

ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc     7740

ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc     7800

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt     7860

cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt     7920

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc     7980

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa     8040

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac     8100

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg     8160

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga     8220

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact     8280

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa     8340

cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc     8400

gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg     8460

ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat     8520

acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt     8580

tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta     8640

ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg     8700

ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc     8760

tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggcctcgagg tcgagatccg     8820

gtcgaccagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca     8880

gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg     8940

ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct     9000

tttgcaaaaa gcttcgacgg tatcgattgg ctcatgtcca acattaccgc catgttgaca     9060

ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata     9120

tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga     9180

cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt     9240

ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt     9300

gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca     9360

ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt     9420

catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt     9480

tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca     9540

ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg     9600

cggtaggcgt gtacggaatt cggagtggcg agccctcaga tcctgcatat aagcagctgc     9660

tttttgcctg tactgggtct ctctg                                           9685


<210>  79
<211>  9721
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R12 intermediate spacer CAR: PJ_R12-CH3-41BB-Z-T2A-tEGFR

<400>  79
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagcgaattc ctcgaggcca ccatgctgct     2100

gctggtgaca agcctgctgc tgtgcgagct gccccacccc gcctttctgc tgatccccca     2160

ggaacagctc gtcgaaagcg gcggcagact ggtgacacct ggcggcagcc tgaccctgag     2220

ctgcaaggcc agcggcttcg acttcagcgc ctactacatg agctgggtcc gccaggcccc     2280

tggcaaggga ctggaatgga tcgccaccat ctaccccagc agcggcaaga cctactacgc     2340

cacctgggtg aacggacggt tcaccatctc cagcgacaac gcccagaaca ccgtggacct     2400

gcagatgaac agcctgacag ccgccgaccg ggccacctac ttttgcgcca gagacagcta     2460

cgccgacgac ggcgccctgt tcaacatctg gggccctggc accctggtga caatctctag     2520

cggcggaggc ggatctggtg gcggaggaag tggcggcgga ggatctgagc tggtgctgac     2580

ccagagcccc tctgtgtctg ctgccctggg aagccctgcc aagatcacct gtaccctgag     2640

cagcgcccac aagaccgaca ccatcgactg gtatcagcag ctgcagggcg aggcccccag     2700

atacctgatg caggtgcaga gcgacggcag ctacaccaag aggccaggcg tgcccgaccg     2760

gttcagcgga tctagctctg gcgccgaccg ctacctgatc atccccagcg tgcaggccga     2820

tgacgaggcc gattactact gtggcgccga ctacatcggc ggctacgtgt tcggcggagg     2880

cacccagctg accgtgaccg gcgagtctaa gtacggaccg ccctgccccc cttgccctgg     2940

ccagcctcgc gagccccagg tgtacaccct gcctccctcc caggaagaga tgaccaagaa     3000

ccaggtgtcc ctgacctgcc tggtgaaggg cttctacccc agcgacatcg ccgtggagtg     3060

ggagagcaac ggccagcctg agaacaacta caagaccacc cctcccgtgc tggacagcga     3120

cggcagcttc ttcctgtaca gccggctgac cgtggacaag agccggtggc aggaaggcaa     3180

cgtctttagc tgcagcgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct     3240

gagcctgtcc ctgggcaaga tgttctgggt gctggtggtg gtgggcgggg tgctggcctg     3300

ctacagcctg ctggtgacag tggccttcat catcttttgg gtgaaacggg gcagaaagaa     3360

actcctgtat atattcaaac aaccatttat gagaccagta caaactactc aagaggaaga     3420

tggctgtagc tgccgatttc cagaagaaga agaaggagga tgtgaactgc gggtgaagtt     3480

cagcagaagc gccgacgccc ctgcctacca gcagggccag aatcagctgt acaacgagct     3540

gaacctgggc agaagggaag agtacgacgt cctggataag cggagaggcc gggaccctga     3600

gatgggcggc aagcctcggc ggaagaaccc ccaggaaggc ctgtataacg aactgcagaa     3660

agacaagatg gccgaggcct acagcgagat cggcatgaag ggcgagcgga ggcggggcaa     3720

gggccacgac ggcctgtatc agggcctgtc caccgccacc aaggatacct acgacgccct     3780

gcacatgcag gccctgcccc caaggctcga gggcggcgga gagggcagag gaagtcttct     3840

aacatgcggt gacgtggagg agaatcccgg ccctaggatg cttctcctgg tgacaagcct     3900

tctgctctgt gagttaccac acccagcatt cctcctgatc ccacgcaaag tgtgtaacgg     3960

aataggtatt ggtgaattta aagactcact ctccataaat gctacgaata ttaaacactt     4020

caaaaactgc acctccatca gtggcgatct ccacatcctg ccggtggcat ttaggggtga     4080

ctccttcaca catactcctc ctctggatcc acaggaactg gatattctga aaaccgtaaa     4140

ggaaatcaca gggtttttgc tgattcaggc ttggcctgaa aacaggacgg acctccatgc     4200

ctttgagaac ctagaaatca tacgcggcag gaccaagcaa catggtcagt tttctcttgc     4260

agtcgtcagc ctgaacataa catccttggg attacgctcc ctcaaggaga taagtgatgg     4320

agatgtgata atttcaggaa acaaaaattt gtgctatgca aatacaataa actggaaaaa     4380

actgtttggg acctccggtc agaaaaccaa aattataagc aacagaggtg aaaacagctg     4440

caaggccaca ggccaggtct gccatgcctt gtgctccccc gagggctgct ggggcccgga     4500

gcccagggac tgcgtctctt gccggaatgt cagccgaggc agggaatgcg tggacaagtg     4560

caaccttctg gagggtgagc caagggagtt tgtggagaac tctgagtgca tacagtgcca     4620

cccagagtgc ctgcctcagg ccatgaacat cacctgcaca ggacggggac cagacaactg     4680

tatccagtgt gcccactaca ttgacggccc ccactgcgtc aagacctgcc cggcaggagt     4740

catgggagaa aacaacaccc tggtctggaa gtacgcagac gccggccatg tgtgccacct     4800

gtgccatcca aactgcacct acggatgcac tgggccaggt cttgaaggct gtccaacgaa     4860

tgggcctaag atcccgtcca tcgccactgg gatggtgggg gccctcctct tgctgctggt     4920

ggtggccctg gggatcggcc tcttcatgtg agcggccgct ctagacccgg gctgcaggaa     4980

ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact     5040

ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg     5100

tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg     5160

ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg     5220

tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg     5280

actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc     5340

tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca     5400

tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc     5460

tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct     5520

ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc     5580

gcctccccgc atcgataccg tcgactagcc gtacctttaa gaccaatgac ttacaaggca     5640

gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc     5700

caaagaagac aagatctgct ttttgcctgt actgggtctc tctggttaga ccagatctga     5760

gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct     5820

tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc     5880

agaccctttt agtcagtgtg gaaaatctct agcagaattc gatatcaagc ttatcgatac     5940

cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacaatt     6000

cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc     6060

gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc     6120

gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt     6180

gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat     6240

cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt     6300

ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt     6360

ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag     6420

gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg     6480

aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc     6540

gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc     6600

gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt     6660

atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct     6720

tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc     6780

cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa     6840

agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg     6900

taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt     6960

tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg     7020

catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac     7080

ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc     7140

ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa     7200

catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc     7260

aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt     7320

aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga     7380

taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa     7440

atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa     7500

gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa     7560

tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt     7620

ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt     7680

gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg     7740

agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt     7800

aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca     7860

agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac     7920

tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac     7980

atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct     8040

taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg     8100

gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca     8160

gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt     8220

aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta     8280

tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc     8340

gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc     8400

cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa     8460

ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag     8520

cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg     8580

ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga     8640

gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat     8700

gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag     8760

ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctgga     8820

gctccaccgc ggtggcggcc tcgaggtcga gatccggtcg accagcaacc atagtcccgc     8880

ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg     8940

gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc     9000

agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt cgacggtatc     9060

gattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata     9120

gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact     9180

tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat     9240

gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta     9300

tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc     9360

tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg     9420

ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg     9480

gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct     9540

ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa     9600

atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggaattcgga     9660

gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct     9720

g                                                                     9721


<210>  80
<211>  10051
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R12 long spacer CAR: PJ_R12-CH2-CH3-41BB-Z-T2A-tEGFR

<400>  80
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagcgaattc ctcgaggcca ccatgctgct     2100

gctggtgaca agcctgctgc tgtgcgagct gccccacccc gcctttctgc tgatccccca     2160

ggaacagctc gtcgaaagcg gcggcagact ggtgacacct ggcggcagcc tgaccctgag     2220

ctgcaaggcc agcggcttcg acttcagcgc ctactacatg agctgggtcc gccaggcccc     2280

tggcaaggga ctggaatgga tcgccaccat ctaccccagc agcggcaaga cctactacgc     2340

cacctgggtg aacggacggt tcaccatctc cagcgacaac gcccagaaca ccgtggacct     2400

gcagatgaac agcctgacag ccgccgaccg ggccacctac ttttgcgcca gagacagcta     2460

cgccgacgac ggcgccctgt tcaacatctg gggccctggc accctggtga caatctctag     2520

cggcggaggc ggatctggtg gcggaggaag tggcggcgga ggatctgagc tggtgctgac     2580

ccagagcccc tctgtgtctg ctgccctggg aagccctgcc aagatcacct gtaccctgag     2640

cagcgcccac aagaccgaca ccatcgactg gtatcagcag ctgcagggcg aggcccccag     2700

atacctgatg caggtgcaga gcgacggcag ctacaccaag aggccaggcg tgcccgaccg     2760

gttcagcgga tctagctctg gcgccgaccg ctacctgatc atccccagcg tgcaggccga     2820

tgacgaggcc gattactact gtggcgccga ctacatcggc ggctacgtgt tcggcggagg     2880

cacccagctg accgtgaccg gcgagtctaa gtacggaccg ccctgccccc cttgccctgc     2940

ccccgagttc ctgggcggac ccagcgtgtt cctgttcccc cccaagccca aggacaccct     3000

gatgatcagc cggacccccg aggtgacctg cgtggtggtg gacgtgagcc aggaagatcc     3060

cgaggtccag ttcaattggt acgtggacgg cgtggaagtg cacaacgcca agaccaagcc     3120

cagagaggaa cagttcaaca gcacctaccg ggtggtgtct gtgctgaccg tgctgcacca     3180

ggactggctg aacggcaaag aatacaagtg caaggtgtcc aacaagggcc tgcccagcag     3240

catcgaaaag accatcagca aggccaaggg ccagcctcgc gagccccagg tgtacaccct     3300

gcctccctcc caggaagaga tgaccaagaa ccaggtgtcc ctgacctgcc tggtgaaggg     3360

cttctacccc agcgacatcg ccgtggagtg ggagagcaac ggccagcctg agaacaacta     3420

caagaccacc cctcccgtgc tggacagcga cggcagcttc ttcctgtaca gccggctgac     3480

cgtggacaag agccggtggc aggaaggcaa cgtctttagc tgcagcgtga tgcacgaggc     3540

cctgcacaac cactacaccc agaagagcct gagcctgtcc ctgggcaaga tgttctgggt     3600

gctggtggtg gtgggcgggg tgctggcctg ctacagcctg ctggtgacag tggccttcat     3660

catcttttgg gtgaaacggg gcagaaagaa actcctgtat atattcaaac aaccatttat     3720

gagaccagta caaactactc aagaggaaga tggctgtagc tgccgatttc cagaagaaga     3780

agaaggagga tgtgaactgc gggtgaagtt cagcagaagc gccgacgccc ctgcctacca     3840

gcagggccag aatcagctgt acaacgagct gaacctgggc agaagggaag agtacgacgt     3900

cctggataag cggagaggcc gggaccctga gatgggcggc aagcctcggc ggaagaaccc     3960

ccaggaaggc ctgtataacg aactgcagaa agacaagatg gccgaggcct acagcgagat     4020

cggcatgaag ggcgagcgga ggcggggcaa gggccacgac ggcctgtatc agggcctgtc     4080

caccgccacc aaggatacct acgacgccct gcacatgcag gccctgcccc caaggctcga     4140

gggcggcgga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg     4200

ccctaggatg cttctcctgg tgacaagcct tctgctctgt gagttaccac acccagcatt     4260

cctcctgatc ccacgcaaag tgtgtaacgg aataggtatt ggtgaattta aagactcact     4320

ctccataaat gctacgaata ttaaacactt caaaaactgc acctccatca gtggcgatct     4380

ccacatcctg ccggtggcat ttaggggtga ctccttcaca catactcctc ctctggatcc     4440

acaggaactg gatattctga aaaccgtaaa ggaaatcaca gggtttttgc tgattcaggc     4500

ttggcctgaa aacaggacgg acctccatgc ctttgagaac ctagaaatca tacgcggcag     4560

gaccaagcaa catggtcagt tttctcttgc agtcgtcagc ctgaacataa catccttggg     4620

attacgctcc ctcaaggaga taagtgatgg agatgtgata atttcaggaa acaaaaattt     4680

gtgctatgca aatacaataa actggaaaaa actgtttggg acctccggtc agaaaaccaa     4740

aattataagc aacagaggtg aaaacagctg caaggccaca ggccaggtct gccatgcctt     4800

gtgctccccc gagggctgct ggggcccgga gcccagggac tgcgtctctt gccggaatgt     4860

cagccgaggc agggaatgcg tggacaagtg caaccttctg gagggtgagc caagggagtt     4920

tgtggagaac tctgagtgca tacagtgcca cccagagtgc ctgcctcagg ccatgaacat     4980

cacctgcaca ggacggggac cagacaactg tatccagtgt gcccactaca ttgacggccc     5040

ccactgcgtc aagacctgcc cggcaggagt catgggagaa aacaacaccc tggtctggaa     5100

gtacgcagac gccggccatg tgtgccacct gtgccatcca aactgcacct acggatgcac     5160

tgggccaggt cttgaaggct gtccaacgaa tgggcctaag atcccgtcca tcgccactgg     5220

gatggtgggg gccctcctct tgctgctggt ggtggccctg gggatcggcc tcttcatgtg     5280

agcggccgct ctagacccgg gctgcaggaa ttcgatatca agcttatcga taatcaacct     5340

ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg     5400

ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc     5460

attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt     5520

gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc     5580

attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg     5640

gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact     5700

gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt     5760

gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg     5820

gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc     5880

cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgactagcc     5940

gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa     6000

aaggggggac tggaagggct aattcactcc caaagaagac aagatctgct ttttgcctgt     6060

actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac     6120

ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg     6180

ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct     6240

agcagaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca     6300

attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact     6360

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct     6420

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg     6480

gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat     6540

cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata     6600

gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt     6660

ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc     6720

atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa     6780

agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg     6840

gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt     6900

aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg     6960

gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc     7020

gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag     7080

tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt     7140

tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt     7200

gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga     7260

acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat     7320

tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga     7380

gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag     7440

tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg     7500

accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg     7560

ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt     7620

agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg     7680

gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc     7740

ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg     7800

tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac     7860

ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact     7920

gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa     7980

acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa     8040

aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg     8100

atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc     8160

gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac     8220

tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca     8280

ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt     8340

ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc     8400

ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg     8460

aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc     8520

cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac     8580

gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct     8640

ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc     8700

cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt     8760

tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac     8820

cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg     8880

cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga     8940

caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac     9000

tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt     9060

gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat     9120

taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc tcgaggtcga     9180

gatccggtcg accagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     9240

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     9300

ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     9360

taggcttttg caaaaagctt cgacggtatc gattggctca tgtccaacat taccgccatg     9420

ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag     9480

cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc     9540

caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg     9600

gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca     9660

tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc     9720

ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt     9780

attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata     9840

gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt     9900

ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca     9960

aatgggcggt aggcgtgtac ggaattcgga gtggcgagcc ctcagatcct gcatataagc    10020

agctgctttt tgcctgtact gggtctctct g                                   10051


<210>  81
<211>  822
<212>  DNA
<213>  Homo sapiens

<400>  81
accatgctgc tgctggtgac aagcctgctg ctgtgcgagc tgccccaccc cgcctttctg       60

ctgatccccc aggaacagct cgtcgaaagc ggcggcagac tggtgacacc tggcggcagc      120

ctgaccctga gctgcaaggc cagcggcttc gacttcagcg cctactacat gagctgggtc      180

cgccaggccc ctggcaaggg actggaatgg atcgccacca tctaccccag cagcggcaag      240

acctactacg ccacctgggt gaacggacgg ttcaccatct ccagcgacaa cgcccagaac      300

accgtggacc tgcagatgaa cagcctgaca gccgccgacc gggccaccta cttttgcgcc      360

agagacagct acgccgacga cggcgccctg ttcaacatct ggggccctgg caccctggtg      420

acaatctcta gcggcggagg cggatctggt ggcggaggaa gtggcggcgg aggatctgag      480

ctggtgctga cccagagccc ctctgtgtct gctgccctgg gaagccctgc caagatcacc      540

tgtaccctga gcagcgccca caagaccgac accatcgact ggtatcagca gctgcagggc      600

gaggccccca gatacctgat gcaggtgcag agcgacggca gctacaccaa gaggccaggc      660

gtgcccgacc ggttcagcgg atctagctct ggcgccgacc gctacctgat catccccagc      720

gtgcaggccg atgacgaggc cgattactac tgtggcgccg actacatcgg cggctacgtg      780

ttcggcggag gcacccagct gaccgtgacc ggcgagtcta ag                         822


<210>  82
<211>  248
<212>  PRT
<213>  Homo sapiens

<400>  82

Gln Glu Gln Leu Val Glu Ser Gly Gly Arg Leu Val Thr Pro Gly Gly 
1               5                   10                  15      


Ser Leu Thr Leu Ser Cys Lys Ala Ser Gly Phe Asp Phe Ser Ala Tyr 
            20                  25                  30          


Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Thr Ile Tyr Pro Ser Ser Gly Lys Thr Tyr Tyr Ala Thr Trp Val 
    50                  55                  60                  


Asn Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Gln Asn Thr Val Asp 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Thr Ala Ala Asp Arg Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Ser Tyr Ala Asp Asp Gly Ala Leu Phe Asn Ile Trp Gly 
            100                 105                 110         


Pro Gly Thr Leu Val Thr Ile Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Val Leu Thr Gln Ser Pro 
    130                 135                 140                 


Ser Val Ser Ala Ala Leu Gly Ser Pro Ala Lys Ile Thr Cys Thr Leu 
145                 150                 155                 160 


Ser Ser Ala His Lys Thr Asp Thr Ile Asp Trp Tyr Gln Gln Leu Gln 
                165                 170                 175     


Gly Glu Ala Pro Arg Tyr Leu Met Gln Val Gln Ser Asp Gly Ser Tyr 
            180                 185                 190         


Thr Lys Arg Pro Gly Val Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
        195                 200                 205             


Ala Asp Arg Tyr Leu Ile Ile Pro Ser Val Gln Ala Asp Asp Glu Ala 
    210                 215                 220                 


Asp Tyr Tyr Cys Gly Ala Asp Tyr Ile Gly Gly Tyr Val Phe Gly Gly 
225                 230                 235                 240 


Gly Thr Gln Leu Thr Val Thr Gly 
                245             


<210>  83
<211>  9384
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R12 short spacer CAR: PJ_R12-Hinge-41BB-Z-T2A-tEGFR

<400>  83
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagaccatgc tgctgctggt gacaagcctg     2100

ctgctgtgcg agctgcccca ccccgccttt ctgctgatcc cccaggaaca gctcgtcgaa     2160

agcggcggca gactggtgac acctggcggc agcctgaccc tgagctgcaa ggccagcggc     2220

ttcgacttca gcgcctacta catgagctgg gtccgccagg cccctggcaa gggactggaa     2280

tggatcgcca ccatctaccc cagcagcggc aagacctact acgccacctg ggtgaacgga     2340

cggttcacca tctccagcga caacgcccag aacaccgtgg acctgcagat gaacagcctg     2400

acagccgccg accgggccac ctacttttgc gccagagaca gctacgccga cgacggcgcc     2460

ctgttcaaca tctggggccc tggcaccctg gtgacaatct ctagcggcgg aggcggatct     2520

ggtggcggag gaagtggcgg cggaggatct gagctggtgc tgacccagag cccctctgtg     2580

tctgctgccc tgggaagccc tgccaagatc acctgtaccc tgagcagcgc ccacaagacc     2640

gacaccatcg actggtatca gcagctgcag ggcgaggccc ccagatacct gatgcaggtg     2700

cagagcgacg gcagctacac caagaggcca ggcgtgcccg accggttcag cggatctagc     2760

tctggcgccg accgctacct gatcatcccc agcgtgcagg ccgatgacga ggccgattac     2820

tactgtggcg ccgactacat cggcggctac gtgttcggcg gaggcaccca gctgaccgtg     2880

accggcgagt ctaagtacgg accgccctgc cccccttgcc ctatgttctg ggtgctggtg     2940

gtggtgggcg gggtgctggc ctgctacagc ctgctggtga cagtggcctt catcatcttt     3000

tgggtgaaac ggggcagaaa gaaactcctg tatatattca aacaaccatt tatgagacca     3060

gtacaaacta ctcaagagga agatggctgt agctgccgat ttccagaaga agaagaagga     3120

ggatgtgaac tgcgggtgaa gttcagcaga agcgccgacg cccctgccta ccagcagggc     3180

cagaatcagc tgtacaacga gctgaacctg ggcagaaggg aagagtacga cgtcctggat     3240

aagcggagag gccgggaccc tgagatgggc ggcaagcctc ggcggaagaa cccccaggaa     3300

ggcctgtata acgaactgca gaaagacaag atggccgagg cctacagcga gatcggcatg     3360

aagggcgagc ggaggcgggg caagggccac gacggcctgt atcagggcct gtccaccgcc     3420

accaaggata cctacgacgc cctgcacatg caggccctgc ccccaaggct cgagggcggc     3480

ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg     3540

atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg     3600

atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata     3660

aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc     3720

ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa     3780

ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct     3840

gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag     3900

caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc     3960

tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat     4020

gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata     4080

agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc     4140

cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga     4200

ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag     4260

aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc     4320

acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc     4380

gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca     4440

gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca     4500

ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg     4560

ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gtgagcggcc     4620

gctctagacc cgggctgcag gaattcgata tcaagcttat cgataatcaa cctctggatt     4680

acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg     4740

gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct     4800

cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc     4860

aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca     4920

ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac     4980

tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt     5040

ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct     5100

ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc     5160

cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga     5220

cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacta gccgtacctt     5280

taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg     5340

gactggaagg gctaattcac tcccaaagaa gacaagatct gctttttgcc tgtactgggt     5400

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     5460

ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg     5520

actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagaa     5580

ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc     5640

ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa     5700

ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa     5760

tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg     5820

gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca     5880

ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag     5940

atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc     6000

aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc     6060

taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc     6120

ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa     6180

gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc     6240

acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat     6300

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     6360

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa     6420

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     6480

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac     6540

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt     6600

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc     6660

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     6720

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc     6780

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     6840

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     6900

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     6960

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     7020

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     7080

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     7140

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     7200

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     7260

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     7320

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     7380

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     7440

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     7500

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     7560

agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc     7620

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     7680

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     7740

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     7800

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     7860

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     7920

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     7980

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     8040

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     8100

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     8160

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata     8220

cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt     8280

cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag     8340

gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga     8400

taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct     8460

cactaaaggg aacaaaagct ggagctccac cgcggtggcg gcctcgaggt cgagatccgg     8520

tcgaccagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag     8580

ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc     8640

cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt     8700

ttgcaaaaag cttcgacggt atcgattggc tcatgtccaa cattaccgcc atgttgacat     8760

tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat     8820

atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac     8880

ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc     8940

cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg     9000

tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat     9060

tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc     9120

atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt     9180

gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac     9240

caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc     9300

ggtaggcgtg tacggaattc ggagtggcga gccctcagat cctgcatata agcagctgct     9360

ttttgcctgt actgggtctc tctg                                            9384


<210>  84
<211>  937
<212>  PRT
<213>  Homo sapiens

<400>  84

Met His Arg Pro Arg Arg Arg Gly Thr Arg Pro Pro Leu Leu Ala Leu 
1               5                   10                  15      


Leu Ala Ala Leu Leu Leu Ala Ala Arg Gly Ala Ala Ala Gln Glu Thr 
            20                  25                  30          


Glu Leu Ser Val Ser Ala Glu Leu Val Pro Thr Ser Ser Trp Asn Ile 
        35                  40                  45              


Ser Ser Glu Leu Asn Lys Asp Ser Tyr Leu Thr Leu Asp Glu Pro Met 
    50                  55                  60                  


Asn Asn Ile Thr Thr Ser Leu Gly Gln Thr Ala Glu Leu His Cys Lys 
65                  70                  75                  80  


Val Ser Gly Asn Pro Pro Pro Thr Ile Arg Trp Phe Lys Asn Asp Ala 
                85                  90                  95      


Pro Val Val Gln Glu Pro Arg Arg Leu Ser Phe Arg Ser Thr Ile Tyr 
            100                 105                 110         


Gly Ser Arg Leu Arg Ile Arg Asn Leu Asp Thr Thr Asp Thr Gly Tyr 
        115                 120                 125             


Phe Gln Cys Val Ala Thr Asn Gly Lys Glu Val Val Ser Ser Thr Gly 
    130                 135                 140                 


Val Leu Phe Val Lys Phe Gly Pro Pro Pro Thr Ala Ser Pro Gly Tyr 
145                 150                 155                 160 


Ser Asp Glu Tyr Glu Glu Asp Gly Phe Cys Gln Pro Tyr Arg Gly Ile 
                165                 170                 175     


Ala Cys Ala Arg Phe Ile Gly Asn Arg Thr Val Tyr Met Glu Ser Leu 
            180                 185                 190         


His Met Gln Gly Glu Ile Glu Asn Gln Ile Thr Ala Ala Phe Thr Met 
        195                 200                 205             


Ile Gly Thr Ser Ser His Leu Ser Asp Lys Cys Ser Gln Phe Ala Ile 
    210                 215                 220                 


Pro Ser Leu Cys His Tyr Ala Phe Pro Tyr Cys Asp Glu Thr Ser Ser 
225                 230                 235                 240 


Val Pro Lys Pro Arg Asp Leu Cys Arg Asp Glu Cys Glu Ile Leu Glu 
                245                 250                 255     


Asn Val Leu Cys Gln Thr Glu Tyr Ile Phe Ala Arg Ser Asn Pro Met 
            260                 265                 270         


Ile Leu Met Arg Leu Lys Leu Pro Asn Cys Glu Asp Leu Pro Gln Pro 
        275                 280                 285             


Glu Ser Pro Glu Ala Ala Asn Cys Ile Arg Ile Gly Ile Pro Met Ala 
    290                 295                 300                 


Asp Pro Ile Asn Lys Asn His Lys Cys Tyr Asn Ser Thr Gly Val Asp 
305                 310                 315                 320 


Tyr Arg Gly Thr Val Ser Val Thr Lys Ser Gly Arg Gln Cys Gln Pro 
                325                 330                 335     


Trp Asn Ser Gln Tyr Pro His Thr His Thr Phe Thr Ala Leu Arg Phe 
            340                 345                 350         


Pro Glu Leu Asn Gly Gly His Ser Tyr Cys Arg Asn Pro Gly Asn Gln 
        355                 360                 365             


Lys Glu Ala Pro Trp Cys Phe Thr Leu Asp Glu Asn Phe Lys Ser Asp 
    370                 375                 380                 


Leu Cys Asp Ile Pro Ala Cys Asp Ser Lys Asp Ser Lys Glu Lys Asn 
385                 390                 395                 400 


Lys Met Glu Ile Leu Tyr Ile Leu Val Pro Ser Val Ala Ile Pro Leu 
                405                 410                 415     


Ala Ile Ala Leu Leu Phe Phe Phe Ile Cys Val Cys Arg Asn Asn Gln 
            420                 425                 430         


Lys Ser Ser Ser Ala Pro Val Gln Arg Gln Pro Lys His Val Arg Gly 
        435                 440                 445             


Gln Asn Val Glu Met Ser Met Leu Asn Ala Tyr Lys Pro Lys Ser Lys 
    450                 455                 460                 


Ala Lys Glu Leu Pro Leu Ser Ala Val Arg Phe Met Glu Glu Leu Gly 
465                 470                 475                 480 


Glu Cys Ala Phe Gly Lys Ile Tyr Lys Gly His Leu Tyr Leu Pro Gly 
                485                 490                 495     


Met Asp His Ala Gln Leu Val Ala Ile Lys Thr Leu Lys Asp Tyr Asn 
            500                 505                 510         


Asn Pro Gln Gln Trp Thr Glu Phe Gln Gln Glu Ala Ser Leu Met Ala 
        515                 520                 525             


Glu Leu His His Pro Asn Ile Val Cys Leu Leu Gly Ala Val Thr Gln 
    530                 535                 540                 


Glu Gln Pro Val Cys Met Leu Phe Glu Tyr Ile Asn Gln Gly Asp Leu 
545                 550                 555                 560 


His Glu Phe Leu Ile Met Arg Ser Pro His Ser Asp Val Gly Cys Ser 
                565                 570                 575     


Ser Asp Glu Asp Gly Thr Val Lys Ser Ser Leu Asp His Gly Asp Phe 
            580                 585                 590         


Leu His Ile Ala Ile Gln Ile Ala Ala Gly Met Glu Tyr Leu Ser Ser 
        595                 600                 605             


His Phe Phe Val His Lys Asp Leu Ala Ala Arg Asn Ile Leu Ile Gly 
    610                 615                 620                 


Glu Gln Leu His Val Lys Ile Ser Asp Leu Gly Leu Ser Arg Glu Ile 
625                 630                 635                 640 


Tyr Ser Ala Asp Tyr Tyr Arg Val Gln Ser Lys Ser Leu Leu Pro Ile 
                645                 650                 655     


Arg Trp Met Pro Pro Glu Ala Ile Met Tyr Gly Lys Phe Ser Ser Asp 
            660                 665                 670         


Ser Asp Ile Trp Ser Phe Gly Val Val Leu Trp Glu Ile Phe Ser Phe 
        675                 680                 685             


Gly Leu Gln Pro Tyr Tyr Gly Phe Ser Asn Gln Glu Val Ile Glu Met 
    690                 695                 700                 


Val Arg Lys Arg Gln Leu Leu Pro Cys Ser Glu Asp Cys Pro Pro Arg 
705                 710                 715                 720 


Met Tyr Ser Leu Met Thr Glu Cys Trp Asn Glu Ile Pro Ser Arg Arg 
                725                 730                 735     


Pro Arg Phe Lys Asp Ile His Val Arg Leu Arg Ser Trp Glu Gly Leu 
            740                 745                 750         


Ser Ser His Thr Ser Ser Thr Thr Pro Ser Gly Gly Asn Ala Thr Thr 
        755                 760                 765             


Gln Thr Thr Ser Leu Ser Ala Ser Pro Val Ser Asn Leu Ser Asn Pro 
    770                 775                 780                 


Arg Tyr Pro Asn Tyr Met Phe Pro Ser Gln Gly Ile Thr Pro Gln Gly 
785                 790                 795                 800 


Gln Ile Ala Gly Phe Ile Gly Pro Pro Ile Pro Gln Asn Gln Arg Phe 
                805                 810                 815     


Ile Pro Ile Asn Gly Tyr Pro Ile Pro Pro Gly Tyr Ala Ala Phe Pro 
            820                 825                 830         


Ala Ala His Tyr Gln Pro Thr Gly Pro Pro Arg Val Ile Gln His Cys 
        835                 840                 845             


Pro Pro Pro Lys Ser Arg Ser Pro Ser Ser Ala Ser Gly Ser Thr Ser 
    850                 855                 860                 


Thr Gly His Val Thr Ser Leu Pro Ser Ser Gly Ser Asn Gln Glu Ala 
865                 870                 875                 880 


Asn Ile Pro Leu Leu Pro His Met Ser Ile Pro Asn His Pro Gly Gly 
                885                 890                 895     


Met Gly Ile Thr Val Phe Gly Asn Lys Ser Gln Lys Pro Tyr Lys Ile 
            900                 905                 910         


Asp Ser Lys Gln Ala Ser Leu Leu Gly Asp Ala Asn Ile His Gly His 
        915                 920                 925             


Thr Glu Ser Met Ile Ser Ala Glu Leu 
    930                 935         


<210>  85
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RRE primer

<400>  85
attgtctggt atagtgcagc ag                                                22


<210>  86
<211>  801
<212>  DNA
<213>  Homo sapiens

<400>  86
gaattcgcca ccatgctgct gctggtgaca agcctgctgc tgtgcgagct gccccacccc       60

gcctttctgc tgatccccca gagcgtgaaa gagtccgagg gcgacctggt cacaccagcc      120

ggcaacctga ccctgacctg taccgccagc ggcagcgaca tcaacgacta ccccatctct      180

tgggtccgcc aggctcctgg caagggactg gaatggatcg gcttcatcaa cagcggcggc      240

agcacttggt acgccagctg ggtcaaaggc cggttcacca tcagccggac cagcaccacc      300

gtggacctga agatgacaag cctgaccacc gacgacaccg ccacctactt ttgcgccaga      360

ggctacagca cctactacgg cgacttcaac atctggggcc ctggcaccct ggtcacaatc      420

tctagcggcg gaggcggcag cggaggtgga ggaagtggcg gcggaggatc cgagctggtc      480

atgacccaga cccccagcag cacatctggc gccgtgggcg gcaccgtgac catcaattgc      540

caggccagcc agagcatcga cagcaacctg gcctggttcc agcagaagcc cggccagccc      600

cccaccctgc tgatctacag agcctccaac ctggccagcg gcgtgccaag cagattcagc      660

ggcagcagat ctggcaccga gtacaccctg accatctccg gcgtgcagag agaggacgcc      720

gctacctatt actgcctggg cggcgtgggc aacgtgtcct acagaaccag cttcggcgga      780

ggtactgagg tggtcgtcaa a                                                801


<210>  87
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 primer

<400>  87
cgaccagcaa ccatagtcc                                                    19


<210>  88
<211>  72
<212>  DNA
<213>  Homo sapiens

<400>  88
ctcgagggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat       60

cccggcccta gg                                                           72


<210>  89
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  89

Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      


Val Glu Glu Asn Pro Gly Pro Arg 
            20                  


<210>  90
<211>  5844
<212>  DNA
<213>  Homo sapiens

<400>  90
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata      120

aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc      180

ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa      240

ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct      300

gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag      360

caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc      420

tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat      480

gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata      540

agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc      600

cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga      660

ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag      720

aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc      780

acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc      840

gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca      900

gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca      960

ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg     1020

ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gtgagcggcc     1080

gctctagacc cgggctgcag gaattcgata tcaagcttat cgataatcaa cctctggatt     1140

acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg     1200

gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct     1260

cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc     1320

aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca     1380

ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac     1440

tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt     1500

ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct     1560

ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc     1620

cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga     1680

cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacta gccgtacctt     1740

taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg     1800

gactggaagg gctaattcac tcccaaagaa gacaagatct gctttttgcc tgtactgggt     1860

ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc     1920

ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg     1980

actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagaa     2040

ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc     2100

ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa     2160

ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa     2220

tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg     2280

gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca     2340

ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag     2400

atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc     2460

aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc     2520

taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc     2580

ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa     2640

gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc     2700

acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat     2760

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     2820

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa     2880

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac     2940

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac     3000

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt     3060

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc     3120

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca     3180

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc     3240

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     3300

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     3360

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     3420

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     3480

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     3540

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     3600

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     3660

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     3720

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     3780

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     3840

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     3900

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     3960

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     4020

agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc     4080

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     4140

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     4200

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     4260

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     4320

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     4380

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     4440

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     4500

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     4560

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     4620

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata     4680

cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt     4740

cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag     4800

gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga     4860

taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct     4920

cactaaaggg aacaaaagct ggagctccac cgcggtggcg gcctcgaggt cgagatccgg     4980

tcgaccagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag     5040

ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc     5100

cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt     5160

ttgcaaaaag cttcgacggt atcgattggc tcatgtccaa cattaccgcc atgttgacat     5220

tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat     5280

atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac     5340

ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc     5400

cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg     5460

tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat     5520

tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc     5580

atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt     5640

gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac     5700

caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc     5760

ggtaggcgtg tacggaattc ggagtggcga gccctcagat cctgcatata agcagctgct     5820

ttttgcctgt actgggtctc tctg                                            5844


<210>  91
<211>  356
<212>  PRT
<213>  Homo sapiens

<400>  91

Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu 
            20                  25                  30          


Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys 
        35                  40                  45              


Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe 
    50                  55                  60                  


Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu 
65                  70                  75                  80  


Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln 
                85                  90                  95      


Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu 
            100                 105                 110         


Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val 
        115                 120                 125             


Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile 
    130                 135                 140                 


Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala 
145                 150                 155                 160 


Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr 
                165                 170                 175     


Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln 
            180                 185                 190         


Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro 
        195                 200                 205             


Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val 
    210                 215                 220                 


Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn 
225                 230                 235                 240 


Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn 
                245                 250                 255     


Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His 
            260                 265                 270         


Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met 
        275                 280                 285             


Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val 
    290                 295                 300                 


Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly 
305                 310                 315                 320 


Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr 
                325                 330                 335     


Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile 
            340                 345                 350         


Gly Leu Phe Met 
        355     


<210>  92
<211>  327
<212>  PRT
<213>  Homo sapiens

<400>  92

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  93
<211>  220
<212>  PRT
<213>  Homo sapiens

<400>  93

Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr 
            20                  25                  30          


Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser 
        35                  40                  45              


Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu 
    50                  55                  60                  


Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser 
65                  70                  75                  80  


Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr 
                85                  90                  95      


Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys 
            100                 105                 110         


Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        115                 120                 125             


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    130                 135                 140                 


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
145                 150                 155                 160 


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                165                 170                 175     


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            180                 185                 190         


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        195                 200                 205             


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
    210                 215                 220 


<210>  94
<211>  164
<212>  PRT
<213>  Homo sapiens

<400>  94

Met Lys Trp Lys Ala Leu Phe Thr Ala Ala Ile Leu Gln Ala Gln Leu 
1               5                   10                  15      


Pro Ile Thr Glu Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys 
            20                  25                  30          


Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu Thr Ala 
        35                  40                  45              


Leu Phe Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    50                  55                  60                  


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
65                  70                  75                  80  


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                85                  90                  95      


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
            100                 105                 110         


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        115                 120                 125             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    130                 135                 140                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
145                 150                 155                 160 


Leu Pro Pro Arg 
                


<210>  95
<211>  240
<212>  PRT
<213>  Homo sapiens

<400>  95

Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu 
1               5                   10                  15      


Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro 
            20                  25                  30          


Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys 
        35                  40                  45              


Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile 
    50                  55                  60                  


Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser 
65                  70                  75                  80  


Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly 
                85                  90                  95      


Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu 
            100                 105                 110         


Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln 
        115                 120                 125             


Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys 
    130                 135                 140                 


Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro 
145                 150                 155                 160 


Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala 
                165                 170                 175     


Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu 
            180                 185                 190         


Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu 
        195                 200                 205             


Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
    210                 215                 220                 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
225                 230                 235                 240 


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE primer

<400>  96
actgtgtttg ctgacgcaac                                                   20


<210>  97
<211>  168
<212>  PRT
<213>  Homo sapiens

<400>  97

Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      


Ile Glu Gly Arg His Met Arg Arg Val Pro Gly Val Ala Pro Thr Leu 
            20                  25                  30          


Val Arg Ser Ala Ser Glu Thr Ser Glu Lys Arg Pro Phe Met Cys Ala 
        35                  40                  45              


Tyr Pro Gly Cys Asn Lys Arg Tyr Phe Lys Leu Ser His Leu Gln Met 
    50                  55                  60                  


His Ser Arg Lys His Thr Gly Glu Lys Pro Tyr Gln Cys Asp Phe Lys 
65                  70                  75                  80  


Asp Cys Glu Arg Arg Phe Phe Arg Ser Asp Gln Leu Lys Arg His Gln 
                85                  90                  95      


Arg Arg His Thr Gly Val Lys Pro Phe Gln Cys Lys Thr Cys Gln Arg 
            100                 105                 110         


Lys Phe Ser Arg Ser Asp His Leu Lys Thr His Thr Arg Thr His Thr 
        115                 120                 125             


Gly Glu Lys Pro Phe Ser Cys Arg Trp Pro Ser Cys Gln Lys Lys Phe 
    130                 135                 140                 


Ala Arg Ser Asp Glu Leu Val Arg His His Asn Met His Gln Arg Asn 
145                 150                 155                 160 


Met Thr Lys Leu Gln Leu Ala Leu 
                165             


<210>  98
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer Region


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa is cysteine, glycine, or arginine

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is cysteine or threonine

<400>  98

Xaa Pro Pro Xaa Pro 
1               5   


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zeta primer

<400>  99
cgggtgaagt tcagcagaag                                                   20


<210>  100
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  100

Ala Asp Arg Ala Thr Tyr Phe Cys Ala 
1               5                   


<210>  101
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  101

Ala Ser Gly Phe Asp Phe Ser Ala Tyr Tyr Met 
1               5                   10      


<210>  102
<211>  6
<212>  PRT
<213>  Homo sapiens

<400>  102

Asp Thr Ile Asp Trp Tyr 
1               5       


<210>  103
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  103

Asp Tyr Gly Val Ser 
1               5   


<210>  104
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  104

Gly Asn Thr Leu Pro Tyr Thr Phe Gly 
1               5                   


<210>  105
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  105

Ile Asn Ser Gly Gly Ser Thr 
1               5           


<210>  106
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  106

Asn Val Ser Tyr Arg Thr Ser Phe 
1               5               


<210>  107
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  107

Arg Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
1               5                   10                  15      


<210>  108
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  108

Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn 
1               5                   10      


<210>  109
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  109

Ser Gly Ser Asp Ile Asn Asp Tyr Pro Ile Ser 
1               5                   10      


<210>  110
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  110

Ser Asn Leu Ala Trp 
1               5   


<210>  111
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  111

Ser Arg Leu His Ser Gly Val 
1               5           


<210>  112
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  112

Thr Ile Tyr Pro Ser Ser Gly 
1               5           


<210>  113
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  113

Val Gln Ser Asp Gly Ser Tyr Thr Lys Arg Pro Gly Val Pro Asp Arg 
1               5                   10                  15      


<210>  114
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  114

Val Thr Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
1               5                   10                  15      


<210>  115
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  115

Tyr Ala Met Asp Tyr Trp Gly 
1               5           


<210>  116
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  116

Tyr Phe Cys Ala Arg Gly Tyr Ser 
1               5               


<210>  117
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  117

Tyr Ile Gly Gly Tyr Val Phe Gly 
1               5               


