                         SEQUENCE LISTING

<110>  INSERM
 
<120>  HUMAN NEUTRALIZING ANTI-KIT ANTIBODIES AND USES THEREOF

<130>  BIO13274POUL/AS

<160>  33    

<170>  PatentIn version 3.3

<210>  1
<211>  123
<212>  PRT
<213>  Artificial

<220>
<223>  2D1 VH chain

<400>  1

Met Ala Gln Val Lys Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp 
            20                  25                  30          


Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Tyr Ile Thr Ser Ser Ser Ser Thr Ile Tyr Tyr Val Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Leu Arg Asn Ser Glu Gly Tyr Trp Tyr Phe Asp Leu 
            100                 105                 110         


Trp Gly Arg Gly Thr Leu Val Thr Val Ser Ser 
        115                 120             


<210>  2
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  2D1 H-CDR1

<400>  2

Gly Phe Thr Phe Asp Ser Tyr Ala 
1               5               


<210>  3
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  2D1 H-CDR2

<400>  3

Ile Thr Ser Ser Ser Ser Thr Ile 
1               5               


<210>  4
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  2 D1 H-CDR3

<400>  4

Arg Leu Arg Asn Ser Glu Gly Tyr Trp Tyr Phe Asp Leu 
1               5                   10              


<210>  5
<211>  110
<212>  PRT
<213>  Artificial

<220>
<223>  2D1 VL chain

<400>  5

Ser Gln Ser Ala Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly 
1               5                   10                  15      


Gln Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Phe 
            20                  25                  30          


Ala Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Leu Leu Val Met 
        35                  40                  45              


Tyr Gly Gln Asn Ile Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Ser Ser Gly Asn Ser Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala 
65                  70                  75                  80  


Glu Asp Glu Ala Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Tyr Asn 
                85                  90                  95      


His Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 110 


<210>  6
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  2D1 L-CDR1

<400>  6

Ser Leu Arg Ser Tyr Phe 
1               5       


<210>  7
<211>  3
<212>  PRT
<213>  Artificial

<220>
<223>  2D1 L-CDR2

<400>  7

Gly Gln Asn 
1           


<210>  8
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  2D1 L-CDR3

<400>  8

Asn Ser Arg Asp Ser Ser Tyr Asn His Trp Val 
1               5                   10      


<210>  9
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 VH chain

<400>  9

Met Ala Gln Val Gln Leu Val Glu Ser Trp Gly Gly Val Ala Gln Pro 
1               5                   10                  15      


Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
            20                  25                  30          


Ser Phe Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ala Val Thr Ser Tyr Asp Gly Ser Asn Glu Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Lys Ala Met Val Arg Gly Val Thr Phe Gly Asp Leu Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  10
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 H-CDR1

<400>  10

Gly Phe Thr Phe Ser Ser Phe Ala 
1               5               


<210>  11
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 H-CDR2

<400>  11

Thr Ser Tyr Asp Gly Ser Asn Glu 
1               5               


<210>  12
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 H-CDR3

<400>  12

Ala Lys Ala Met Val Arg Gly Val Thr Phe Gly Asp Leu Asp Tyr 
1               5                   10                  15  


<210>  13
<211>  110
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 VL chain

<400>  13

Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala 
            20                  25                  30          


Ser Trp Tyr Gln Gln Lys Pro Glu Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Gly Glu Asn Ser Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser 
    50                  55                  60                  


Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Thr His 
                85                  90                  95      


Leu Arg Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 110 


<210>  14
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 L-CDR1

<400>  14

Ser Leu Arg Ser Tyr Tyr 
1               5       


<210>  15
<211>  3
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 L-CDR2

<400>  15

Gly Glu Asn 
1           


<210>  16
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 L-CDR3

<400>  16

Asn Ser Arg Asp Ser Ser Gly Thr His Leu Arg Val 
1               5                   10          


<210>  17
<211>  247
<212>  PRT
<213>  Artificial

<220>
<223>  2D1  VH-linker-VL

<400>  17

Met Ala Gln Val Lys Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp 
            20                  25                  30          


Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Tyr Ile Thr Ser Ser Ser Ser Thr Ile Tyr Tyr Val Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Leu Arg Asn Ser Glu Gly Tyr Trp Tyr Phe Asp Leu 
            100                 105                 110         


Trp Gly Arg Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Ala Leu Thr Gln 
    130                 135                 140                 


Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg Ile Thr Cys 
145                 150                 155                 160 


Gln Gly Asp Ser Leu Arg Ser Tyr Phe Ala Ser Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Gln Ala Pro Leu Leu Val Met Tyr Gly Gln Asn Ile Arg Pro 
            180                 185                 190         


Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Asn Ser Ala 
        195                 200                 205             


Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala Asp Tyr Tyr 
    210                 215                 220                 


Cys Asn Ser Arg Asp Ser Ser Tyr Asn His Trp Val Phe Gly Gly Gly 
225                 230                 235                 240 


Thr Lys Leu Thr Val Leu Gly 
                245         


<210>  18
<211>  248
<212>  PRT
<213>  Artificial

<220>
<223>  3G1 VH-linker-VL

<400>  18

Met Ala Gln Val Gln Leu Val Glu Ser Trp Gly Gly Val Ala Gln Pro 
1               5                   10                  15      


Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
            20                  25                  30          


Ser Phe Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ala Val Thr Ser Tyr Asp Gly Ser Asn Glu Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Lys Ala Met Val Arg Gly Val Thr Phe Gly Asp Leu Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu Leu Thr Gln 
    130                 135                 140                 


Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg Ile Thr Cys 
145                 150                 155                 160 


Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Glu Gln Ala Pro Val Leu Val Ile Tyr Gly Glu Asn Ser Arg Pro 
            180                 185                 190         


Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Asn Thr Ala 
        195                 200                 205             


Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala Asp Tyr Tyr 
    210                 215                 220                 


Cys Asn Ser Arg Asp Ser Ser Gly Thr His Leu Arg Val Phe Gly Gly 
225                 230                 235                 240 


Gly Thr Lys Leu Thr Val Leu Gly 
                245             


<210>  19
<211>  369
<212>  DNA
<213>  Artificial

<220>
<223>  2D1 VH chain

<400>  19
atggcccagg tcaagctgca ggagtctggg ggaggcttgg tacagcctgg ggggtccctg     60

agactctcct gtgcagcctc tggattcacc tttgacagct atgccatgag ctgggtccgc    120

caggctccag ggaaggggct ggagtgggtt tcatacatta ctagtagtag tagtaccata    180

tactacgtag actctgtgaa gggccgattc accatctcca gagacaatgc caagaactca    240

ctgtatctgc aaatgaacag cctgagagac gaggacacgg ctgtgtatta ctgtgcgaga    300

ctccgtaact ccgagggata ctggtacttc gatctctggg gccgtggcac cctggtcacc    360

gtctcctca                                                            369


<210>  20
<211>  330
<212>  DNA
<213>  Artificial

<220>
<223>  2D1 VL chain

<400>  20
tcgcagtctg ctctgactca ggaccctgct gtgtctgtgg ccttgggaca gacagtcagg     60

atcacatgcc aaggagacag cctcagaagc tattttgcaa gttggtacca gcagaagcca    120

ggacaggccc ctctccttgt catgtatggt caaaacatcc ggccctcagg gatcccagac    180

cgattctctg gctccagctc aggaaactca gcttccttga ccatcactgg ggctcaggcg    240

gaagatgagg ctgactatta ctgtaactcc cgggacagca gttataacca ttgggtgttc    300

ggcggaggga ccaagctgac cgtcctaggt                                     330


<210>  21
<211>  741
<212>  DNA
<213>  Artificial

<220>
<223>  2D1 VH-linker-VL

<400>  21
atggcccagg tcaagctgca ggagtctggg ggaggcttgg tacagcctgg ggggtccctg     60

agactctcct gtgcagcctc tggattcacc tttgacagct atgccatgag ctgggtccgc    120

caggctccag ggaaggggct ggagtgggtt tcatacatta ctagtagtag tagtaccata    180

tactacgtag actctgtgaa gggccgattc accatctcca gagacaatgc caagaactca    240

ctgtatctgc aaatgaacag cctgagagac gaggacacgg ctgtgtatta ctgtgcgaga    300

ctccgtaact ccgagggata ctggtacttc gatctctggg gccgtggcac cctggtcacc    360

gtctcctcag gtggaggcgg ttcaggcgga ggtggctctg gcggtggcgg atcgcagtct    420

gctctgactc aggaccctgc tgtgtctgtg gccttgggac agacagtcag gatcacatgc    480

caaggagaca gcctcagaag ctattttgca agttggtacc agcagaagcc aggacaggcc    540

cctctccttg tcatgtatgg tcaaaacatc cggccctcag ggatcccaga ccgattctct    600

ggctccagct caggaaactc agcttccttg accatcactg gggctcaggc ggaagatgag    660

gctgactatt actgtaactc ccgggacagc agttataacc attgggtgtt cggcggaggg    720

accaagctga ccgtcctagg t                                              741


<210>  22
<211>  372
<212>  DNA
<213>  Artificial

<220>
<223>  3G1 VH chain

<400>  22
atggcccagg tgcagctggt ggagtcttgg ggaggcgtgg cccagcctgg gaggtccctg     60

agactctcct gtgcagcctc tggattcacc ttcagtagtt ttgccatgca ctgggtccgc    120

caggctccag gcaaggggct ggagtgggtg gcagttacat catatgatgg aagtaatgaa    180

tactatgcag actccgtgaa gggccgattc accatctcca gagacaattc caagaacacg    240

ctgtatctgc aaatgaacag cctgagagct gaggacacgg ctgtgtatta ctgtgcgaaa    300

gctatggttc ggggagttac gtttggcgac cttgactact ggggccaggg aaccctggtc    360

accgtctcct ca                                                        372


<210>  23
<211>  330
<212>  DNA
<213>  Artificial

<220>
<223>  3G1 VL chain

<400>  23
tcgtctgagc tgactcagga ccctgctgtg tctgtggcct tgggacagac agtcagaatc     60

acatgccaag gagacagcct cagaagttat tatgcaagct ggtaccagca gaagccagaa    120

caggcccctg tacttgtcat ctatggtgaa aacagccggc cctcagggat cccagaccga    180

ttctctggct ccagctcagg aaacacagct tccttgacca tcactggggc tcaggcggaa    240

gatgaggctg actattactg caactctcgc gacagcagtg gtacccatct aagggtattc    300

ggcggaggga ccaagctgac cgtcctaggt                                     330


<210>  24
<211>  744
<212>  DNA
<213>  Artificial

<220>
<223>  3G1 VH-linker-VL

<400>  24
atggcccagg tgcagctggt ggagtcttgg ggaggcgtgg cccagcctgg gaggtccctg     60

agactctcct gtgcagcctc tggattcacc ttcagtagtt ttgccatgca ctgggtccgc    120

caggctccag gcaaggggct ggagtgggtg gcagttacat catatgatgg aagtaatgaa    180

tactatgcag actccgtgaa gggccgattc accatctcca gagacaattc caagaacacg    240

ctgtatctgc aaatgaacag cctgagagct gaggacacgg ctgtgtatta ctgtgcgaaa    300

gctatggttc ggggagttac gtttggcgac cttgactact ggggccaggg aaccctggtc    360

accgtctcct caggtggagg cggttcaggc ggaggtggct ctggcggtgg cggatcgtct    420

gagctgactc aggaccctgc tgtgtctgtg gccttgggac agacagtcag aatcacatgc    480

caaggagaca gcctcagaag ttattatgca agctggtacc agcagaagcc agaacaggcc    540

cctgtacttg tcatctatgg tgaaaacagc cggccctcag ggatcccaga ccgattctct    600

ggctccagct caggaaacac agcttccttg accatcactg gggctcaggc ggaagatgag    660

gctgactatt actgcaactc tcgcgacagc agtggtaccc atctaagggt attcggcgga    720

gggaccaagc tgaccgtcct aggt                                           744


<210>  25
<211>  106
<212>  PRT
<213>  Homo sapiens

<400>  25

Asn Lys Pro Glu Ile Leu Thr Tyr Asp Arg Leu Val Asn Gly Met Leu 
1               5                   10                  15      


Gln Cys Val Ala Ala Gly Phe Pro Glu Pro Thr Ile Asp Trp Tyr Phe 
            20                  25                  30          


Cys Pro Gly Thr Glu Gln Arg Cys Ser Ala Ser Val Leu Pro Val Asp 
        35                  40                  45              


Val Gln Thr Leu Asn Ser Ser Gly Pro Pro Phe Gly Lys Leu Val Val 
    50                  55                  60                  


Gln Ser Ser Ile Asp Ser Ser Ala Phe Lys His Asn Gly Thr Val Glu 
65                  70                  75                  80  


Cys Lys Ala Tyr Asn Asp Val Gly Lys Thr Ser Ala Tyr Phe Asn Phe 
                85                  90                  95      


Ala Phe Lys Glu Gln Ile His Pro His Thr 
            100                 105     


<210>  26
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  2D1-C7 VL chain

<400>  26

Ser Gln Ser Val Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly 
1               5                   10                  15      


Gln Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr 
            20                  25                  30          


Ala Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Leu Leu Val Met 
        35                  40                  45              


Tyr Gly Glu Asn Ile Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Thr Ser Gly Asn Ser Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala 
65                  70                  75                  80  


Glu Asp Glu Ala Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn 
                85                  90                  95      


His Leu Asn Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 110         


<210>  27
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  2D1-C7 L-CDR1

<400>  27

Ser Leu Arg Ser Tyr Tyr 
1               5       


<210>  28
<211>  3
<212>  PRT
<213>  Artificial

<220>
<223>  2D1-C7 L-CDR2

<400>  28

Gly Glu Asn 
1           


<210>  29
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  2D1-C7 L-CDR3

<400>  29

Asn Ser Arg Asp Ser Ser Gly Asn His Leu Asn Trp Val 
1               5                   10              


<210>  30
<211>  109
<212>  PRT
<213>  Artificial

<220>
<223>  3G1-A2 VL chain

<400>  30

Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Val Arg Lys Thr Cys Gln Gly Asp Ser Leu Lys Ser Tyr Tyr Ala 
            20                  25                  30          


Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Gly Glu Asn Ser Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser 
    50                  55                  60                  


Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Cys Ser Arg Ala Thr Gly Gly Tyr His 
                85                  90                  95      


Arg Ile Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 


<210>  31
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  3G1-A2 L-CDR1

<400>  31

Ser Leu Lys Ser Tyr Tyr 
1               5       


<210>  32
<211>  3
<212>  PRT
<213>  Artificial

<220>
<223>  3G1-A2 L-CDR2

<400>  32

Gly Glu Asn 
1           


<210>  33
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  3G1-A2 L-CDR3

<400>  33

Cys Ser Arg Ala Thr Gly Gly Tyr His Arg Ile 
1               5                   10      


