                         SEQUENCE LISTING

<110>  New York Blood Center, Inc.
       Ginzburg, Yelena Z.
 
<120>  METHODS AND COMPOSITIONS FOR INCREASING HEPCIDIN EXPRESSION USING
        MODIFIED IRON BINDING/RELEASING TRANSFERRIN

<130>  1958427-00202WO

<150>  US 60/900,222
<151>  2013-11-05

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  2808
<212>  DNA
<213>  Homo sapiens

<400>  1
tggcaccgag cgagccgcga tgacaatggc tgcattgtgc ttcatgtccc ttcccatcaa       60

catttctgtg ctggactcct tccactcgcg ggtcgtctcc agagctcaga aaatgaggtg      120

atcagtggga cgagtaagga aggggggttg ggagaggggc gattgggcaa cccggctgca      180

caaacacggg aggtcaaaga ttgcgcccag cccgcccagg ccgggaatgg aataaaggga      240

cgcggggcgc cggaggctgc acagaagcga gtccgactgt gctcgctgct cagcgccgca      300

cccggaagat gaggctcgcc gtgggagccc tgctggtctg cgccgtcctg gggctgtgtc      360

tggctgtccc tgataaaact gtgagatggt gtgcagtgtc ggagcatgag gccactaagt      420

gccagagttt ccgcgaccat atgaaaagcg tcattccatc cgatggtccc agtgttgctt      480

gtgtgaagaa agcctcctac cttgattgca tcagggccat tgcggcaaac gaagcggatg      540

ctgtgacact ggatgcaggt ttggtgtatg atgcttacct ggctcccaat aacctgaagc      600

ctgtggtggc agagttctat gggtcaaaag aggatccaca gactttctat tatgctgttg      660

ctgtggtgaa gaaggatagt ggcttccaga tgaaccagct tcgaggcaag aagtcctgcc      720

acacgggtct aggcaggtcc gctgggtgga acatccccat aggcttactt tactgtgact      780

tacctgagcc acgtaaacct cttgagaaag cagtggccaa tttcttctcg ggcagctgtg      840

ccccttgtgc ggatgggacg gacttccccc agctgtgtca actgtgtcca gggtgtggct      900

gctccaccct taaccaatac ttcggctact cgggagcctt caagtgtctg aaggatggtg      960

ctggggatgt ggcctttgtc aagcactcga ctatatttga gaacttggca aacaaggctg     1020

acagggacca gtatgagctg ctttgcctgg acaacacccg gaagccggta gatgaataca     1080

aggactgcca cttggcccag gtcccttctc ataccgtcgt ggcccgaagt atgggcggca     1140

aggaggactt gatctgggag cttctcaacc aggcccagga acattttggc aaagacaaat     1200

caaaagaatt ccaactattc agctctcctc atgggaagga cctgctgttt aaggactctg     1260

cccacgggtt tttaaaagtc ccccccagga tggatgccaa gatgtacctg ggctatgagt     1320

atgtcactgc catccggaat ctacgggaag gcacatgccc agaagcccca acagatgaat     1380

gcaagcctgt gaagtggtgt gcgctgagcc accacgagag gctcaagtgt gatgagtgga     1440

gtgttaacag tgtagggaaa atagagtgtg tatcagcaga gaccaccgaa gactgcatcg     1500

ccaagatcat gaatggagaa gctgatgcca tgagcttgga tggagggttt gtctacatag     1560

cgggcaagtg tggtctggtg cctgtcttgg cagaaaacta caataagagc gataattgtg     1620

aggatacacc agaggcaggg tattttgctg tagcagtggt gaagaaatca gcttctgacc     1680

tcacctggga caatctgaaa ggcaagaagt cctgccatac ggcagttggc agaaccgctg     1740

gctggaacat ccccatgggc ctgctctaca ataagatcaa ccactgcaga tttgatgaat     1800

ttttcagtga aggttgtgcc cctgggtcta agaaagactc cagtctctgt aagctgtgta     1860

tgggctcagg cctaaacctg tgtgaaccca acaacaaaga gggatactac ggctacacag     1920

gcgctttcag gtgtctggtt gagaagggag atgtggcctt tgtgaaacac cagactgtcc     1980

cacagaacac tgggggaaaa aaccctgatc catgggctaa gaatctgaat gaaaaagact     2040

atgagttgct gtgccttgat ggtaccagga aacctgtgga ggagtatgcg aactgccacc     2100

tggccagagc cccgaatcac gctgtggtca cacggaaaga taaggaagct tgcgtccaca     2160

agatattacg tcaacagcag cacctatttg gaagcaacgt aactgactgc tcgggcaact     2220

tttgtttgtt ccggtcggaa accaaggacc ttctgttcag agatgacaca gtatgtttgg     2280

ccaaacttca tgacagaaac acatatgaaa aatacttagg agaagaatat gtcaaggctg     2340

ttggtaacct gagaaaatgc tccacctcat cactcctgga agcctgcact ttccgtagac     2400

cttaaaatct cagaggtagg gctgccacca aggtgaagat gggaacgcag atgatccatg     2460

agtttgccct ggtttcactg gcccaagtgg tttgtgctaa ccacgtctgt cttcacagct     2520

ctgtgttgcc atgtgtgctg aacaaaaaat aaaaattatt attgatttta tatttcaaaa     2580

actccattct ttcctaaata ttttcaacaa aggatttctt tatgcattct gcctaaatac     2640

ctatgcaact gagcccttcc ttctcagctc aagattcgtc tggtctttcc ctacagcttt     2700

gtgtgtgcca tggccacatc tcctgggtac agttcaagga gacatctttt ctaaaagggt     2760

ctgcgtgatc attaaaatat aatcaaatgt aaaaaaaaaa aaaaaaaa                  2808


<210>  2
<211>  698
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Arg Leu Ala Val Gly Ala Leu Leu Val Cys Ala Val Leu Gly Leu 
1               5                   10                  15      


Cys Leu Ala Val Pro Asp Lys Thr Val Arg Trp Cys Ala Val Ser Glu 
            20                  25                  30          


His Glu Ala Thr Lys Cys Gln Ser Phe Arg Asp His Met Lys Ser Val 
        35                  40                  45              


Ile Pro Ser Asp Gly Pro Ser Val Ala Cys Val Lys Lys Ala Ser Tyr 
    50                  55                  60                  


Leu Asp Cys Ile Arg Ala Ile Ala Ala Asn Glu Ala Asp Ala Val Thr 
65                  70                  75                  80  


Leu Asp Ala Gly Leu Val Tyr Asp Ala Tyr Leu Ala Pro Asn Asn Leu 
                85                  90                  95      


Lys Pro Val Val Ala Glu Phe Tyr Gly Ser Lys Glu Asp Pro Gln Thr 
            100                 105                 110         


Phe Tyr Tyr Ala Val Ala Val Val Lys Lys Asp Ser Gly Phe Gln Met 
        115                 120                 125             


Asn Gln Leu Arg Gly Lys Lys Ser Cys His Thr Gly Leu Gly Arg Ser 
    130                 135                 140                 


Ala Gly Trp Asn Ile Pro Ile Gly Leu Leu Tyr Cys Asp Leu Pro Glu 
145                 150                 155                 160 


Pro Arg Lys Pro Leu Glu Lys Ala Val Ala Asn Phe Phe Ser Gly Ser 
                165                 170                 175     


Cys Ala Pro Cys Ala Asp Gly Thr Asp Phe Pro Gln Leu Cys Gln Leu 
            180                 185                 190         


Cys Pro Gly Cys Gly Cys Ser Thr Leu Asn Gln Tyr Phe Gly Tyr Ser 
        195                 200                 205             


Gly Ala Phe Lys Cys Leu Lys Asp Gly Ala Gly Asp Val Ala Phe Val 
    210                 215                 220                 


Lys His Ser Thr Ile Phe Glu Asn Leu Ala Asn Lys Ala Asp Arg Asp 
225                 230                 235                 240 


Gln Tyr Glu Leu Leu Cys Leu Asp Asn Thr Arg Lys Pro Val Asp Glu 
                245                 250                 255     


Tyr Lys Asp Cys His Leu Ala Gln Val Pro Ser His Thr Val Val Ala 
            260                 265                 270         


Arg Ser Met Gly Gly Lys Glu Asp Leu Ile Trp Glu Leu Leu Asn Gln 
        275                 280                 285             


Ala Gln Glu His Phe Gly Lys Asp Lys Ser Lys Glu Phe Gln Leu Phe 
    290                 295                 300                 


Ser Ser Pro His Gly Lys Asp Leu Leu Phe Lys Asp Ser Ala His Gly 
305                 310                 315                 320 


Phe Leu Lys Val Pro Pro Arg Met Asp Ala Lys Met Tyr Leu Gly Tyr 
                325                 330                 335     


Glu Tyr Val Thr Ala Ile Arg Asn Leu Arg Glu Gly Thr Cys Pro Glu 
            340                 345                 350         


Ala Pro Thr Asp Glu Cys Lys Pro Val Lys Trp Cys Ala Leu Ser His 
        355                 360                 365             


His Glu Arg Leu Lys Cys Asp Glu Trp Ser Val Asn Ser Val Gly Lys 
    370                 375                 380                 


Ile Glu Cys Val Ser Ala Glu Thr Thr Glu Asp Cys Ile Ala Lys Ile 
385                 390                 395                 400 


Met Asn Gly Glu Ala Asp Ala Met Ser Leu Asp Gly Gly Phe Val Tyr 
                405                 410                 415     


Ile Ala Gly Lys Cys Gly Leu Val Pro Val Leu Ala Glu Asn Tyr Asn 
            420                 425                 430         


Lys Ser Asp Asn Cys Glu Asp Thr Pro Glu Ala Gly Tyr Phe Ala Val 
        435                 440                 445             


Ala Val Val Lys Lys Ser Ala Ser Asp Leu Thr Trp Asp Asn Leu Lys 
    450                 455                 460                 


Gly Lys Lys Ser Cys His Thr Ala Val Gly Arg Thr Ala Gly Trp Asn 
465                 470                 475                 480 


Ile Pro Met Gly Leu Leu Tyr Asn Lys Ile Asn His Cys Arg Phe Asp 
                485                 490                 495     


Glu Phe Phe Ser Glu Gly Cys Ala Pro Gly Ser Lys Lys Asp Ser Ser 
            500                 505                 510         


Leu Cys Lys Leu Cys Met Gly Ser Gly Leu Asn Leu Cys Glu Pro Asn 
        515                 520                 525             


Asn Lys Glu Gly Tyr Tyr Gly Tyr Thr Gly Ala Phe Arg Cys Leu Val 
    530                 535                 540                 


Glu Lys Gly Asp Val Ala Phe Val Lys His Gln Thr Val Pro Gln Asn 
545                 550                 555                 560 


Thr Gly Gly Lys Asn Pro Asp Pro Trp Ala Lys Asn Leu Asn Glu Lys 
                565                 570                 575     


Asp Tyr Glu Leu Leu Cys Leu Asp Gly Thr Arg Lys Pro Val Glu Glu 
            580                 585                 590         


Tyr Ala Asn Cys His Leu Ala Arg Ala Pro Asn His Ala Val Val Thr 
        595                 600                 605             


Arg Lys Asp Lys Glu Ala Cys Val His Lys Ile Leu Arg Gln Gln Gln 
    610                 615                 620                 


His Leu Phe Gly Ser Asn Val Thr Asp Cys Ser Gly Asn Phe Cys Leu 
625                 630                 635                 640 


Phe Arg Ser Glu Thr Lys Asp Leu Leu Phe Arg Asp Asp Thr Val Cys 
                645                 650                 655     


Leu Ala Lys Leu His Asp Arg Asn Thr Tyr Glu Lys Tyr Leu Gly Glu 
            660                 665                 670         


Glu Tyr Val Lys Ala Val Gly Asn Leu Arg Lys Cys Ser Thr Ser Ser 
        675                 680                 685             


Leu Leu Glu Ala Cys Thr Phe Arg Arg Pro 
    690                 695             


<210>  3
<211>  679
<212>  PRT
<213>  Homo sapiens

<400>  3

Val Pro Asp Lys Thr Val Arg Trp Cys Ala Val Ser Glu His Glu Ala 
1               5                   10                  15      


Thr Lys Cys Gln Ser Phe Arg Asp His Met Lys Ser Val Ile Pro Ser 
            20                  25                  30          


Asp Gly Pro Ser Val Ala Cys Val Lys Lys Ala Ser Tyr Leu Asp Cys 
        35                  40                  45              


Ile Arg Ala Ile Ala Ala Asn Glu Ala Asp Ala Val Thr Leu Asp Ala 
    50                  55                  60                  


Gly Leu Val Tyr Asp Ala Tyr Leu Ala Pro Asn Asn Leu Lys Pro Val 
65                  70                  75                  80  


Val Ala Glu Phe Tyr Gly Ser Lys Glu Asp Pro Gln Thr Phe Tyr Tyr 
                85                  90                  95      


Ala Val Ala Val Val Lys Lys Asp Ser Gly Phe Gln Met Asn Gln Leu 
            100                 105                 110         


Arg Gly Lys Lys Ser Cys His Thr Gly Leu Gly Arg Ser Ala Gly Trp 
        115                 120                 125             


Asn Ile Pro Ile Gly Leu Leu Tyr Cys Asp Leu Pro Glu Pro Arg Lys 
    130                 135                 140                 


Pro Leu Glu Lys Ala Val Ala Asn Phe Phe Ser Gly Ser Cys Ala Pro 
145                 150                 155                 160 


Cys Ala Asp Gly Thr Asp Phe Pro Gln Leu Cys Gln Leu Cys Pro Gly 
                165                 170                 175     


Cys Gly Cys Ser Thr Leu Asn Gln Tyr Phe Gly Tyr Ser Gly Ala Phe 
            180                 185                 190         


Lys Cys Leu Lys Asp Gly Ala Gly Asp Val Ala Phe Val Lys His Ser 
        195                 200                 205             


Thr Ile Phe Glu Asn Leu Ala Asn Lys Ala Asp Arg Asp Gln Tyr Glu 
    210                 215                 220                 


Leu Leu Cys Leu Asp Asn Thr Arg Lys Pro Val Asp Glu Tyr Lys Asp 
225                 230                 235                 240 


Cys His Leu Ala Gln Val Pro Ser His Thr Val Val Ala Arg Ser Met 
                245                 250                 255     


Gly Gly Lys Glu Asp Leu Ile Trp Glu Leu Leu Asn Gln Ala Gln Glu 
            260                 265                 270         


His Phe Gly Lys Asp Lys Ser Lys Glu Phe Gln Leu Phe Ser Ser Pro 
        275                 280                 285             


His Gly Lys Asp Leu Leu Phe Lys Asp Ser Ala His Gly Phe Leu Lys 
    290                 295                 300                 


Val Pro Pro Arg Met Asp Ala Lys Met Tyr Leu Gly Tyr Glu Tyr Val 
305                 310                 315                 320 


Thr Ala Ile Arg Asn Leu Arg Glu Gly Thr Cys Pro Glu Ala Pro Thr 
                325                 330                 335     


Asp Glu Cys Lys Pro Val Lys Trp Cys Ala Leu Ser His His Glu Arg 
            340                 345                 350         


Leu Lys Cys Asp Glu Trp Ser Val Asn Ser Val Gly Lys Ile Glu Cys 
        355                 360                 365             


Val Ser Ala Glu Thr Thr Glu Asp Cys Ile Ala Lys Ile Met Asn Gly 
    370                 375                 380                 


Glu Ala Asp Ala Met Ser Leu Asp Gly Gly Phe Val Tyr Ile Ala Gly 
385                 390                 395                 400 


Lys Cys Gly Leu Val Pro Val Leu Ala Glu Asn Tyr Asn Lys Ser Asp 
                405                 410                 415     


Asn Cys Glu Asp Thr Pro Glu Ala Gly Tyr Phe Ala Val Ala Val Val 
            420                 425                 430         


Lys Lys Ser Ala Ser Asp Leu Thr Trp Asp Asn Leu Lys Gly Lys Lys 
        435                 440                 445             


Ser Cys His Thr Ala Val Gly Arg Thr Ala Gly Trp Asn Ile Pro Met 
    450                 455                 460                 


Gly Leu Leu Tyr Asn Lys Ile Asn His Cys Arg Phe Asp Glu Phe Phe 
465                 470                 475                 480 


Ser Glu Gly Cys Ala Pro Gly Ser Lys Lys Asp Ser Ser Leu Cys Lys 
                485                 490                 495     


Leu Cys Met Gly Ser Gly Leu Asn Leu Cys Glu Pro Asn Asn Lys Glu 
            500                 505                 510         


Gly Tyr Tyr Gly Tyr Thr Gly Ala Phe Arg Cys Leu Val Glu Lys Gly 
        515                 520                 525             


Asp Val Ala Phe Val Lys His Gln Thr Val Pro Gln Asn Thr Gly Gly 
    530                 535                 540                 


Lys Asn Pro Asp Pro Trp Ala Lys Asn Leu Asn Glu Lys Asp Tyr Glu 
545                 550                 555                 560 


Leu Leu Cys Leu Asp Gly Thr Arg Lys Pro Val Glu Glu Tyr Ala Asn 
                565                 570                 575     


Cys His Leu Ala Arg Ala Pro Asn His Ala Val Val Thr Arg Lys Asp 
            580                 585                 590         


Lys Glu Ala Cys Val His Lys Ile Leu Arg Gln Gln Gln His Leu Phe 
        595                 600                 605             


Gly Ser Asn Val Thr Asp Cys Ser Gly Asn Phe Cys Leu Phe Arg Ser 
    610                 615                 620                 


Glu Thr Lys Asp Leu Leu Phe Arg Asp Asp Thr Val Cys Leu Ala Lys 
625                 630                 635                 640 


Leu His Asp Arg Asn Thr Tyr Glu Lys Tyr Leu Gly Glu Glu Tyr Val 
                645                 650                 655     


Lys Ala Val Gly Asn Leu Arg Lys Cys Ser Thr Ser Ser Leu Leu Glu 
            660                 665                 670         


Ala Cys Thr Phe Arg Arg Pro 
        675                 


