                         SEQUENCE LISTING

<110>  UCL Business PLC
 
<120>  T Cell

<130>  P103294PCT

<150>  GB 1410934.2
<151>  2014-06-19

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  1129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric antigen receptor (CAR)

<400>  1

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        755                 760                 765             


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    770                 775                 780                 


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
785                 790                 795                 800 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                805                 810                 815     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            820                 825                 830         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        835                 840                 845             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    850                 855                 860                 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
865                 870                 875                 880 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
                885                 890                 895     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            900                 905                 910         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        915                 920                 925             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    930                 935                 940                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
945                 950                 955                 960 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                965                 970                 975     


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe Trp Val Leu 
            980                 985                 990         


Val Val Val Gly Gly Val Leu Ala  Cys Tyr Ser Leu Leu  Val Thr Val 
        995                 1000                 1005             


Ala Phe  Ile Ile Phe Trp Val  Arg Ser Arg Val Lys  Phe Ser Arg 
    1010                 1015                 1020             


Ser Ala  Asp Ala Pro Ala Tyr  Gln Gln Gly Gln Asn  Gln Leu Tyr 
    1025                 1030                 1035             


Asn Glu  Leu Asn Leu Gly Arg  Arg Glu Glu Tyr Asp  Val Leu Asp 
    1040                 1045                 1050             


Lys Arg  Arg Gly Arg Asp Pro  Glu Met Gly Gly Lys  Pro Arg Arg 
    1055                 1060                 1065             


Lys Asn  Pro Gln Glu Gly Leu  Tyr Asn Glu Leu Gln  Lys Asp Lys 
    1070                 1075                 1080             


Met Ala  Glu Ala Tyr Ser Glu  Ile Gly Met Lys Gly  Glu Arg Arg 
    1085                 1090                 1095             


Arg Gly  Lys Gly His Asp Gly  Leu Tyr Gln Gly Leu  Ser Thr Ala 
    1100                 1105                 1110             


Thr Lys  Asp Thr Tyr Asp Ala  Leu His Met Gln Ala  Leu Pro Pro 
    1115                 1120                 1125             


Arg 
    


<210>  2
<211>  1350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric antigen receptor (CAR)

<400>  2

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        755                 760                 765             


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    770                 775                 780                 


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
785                 790                 795                 800 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                805                 810                 815     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            820                 825                 830         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        835                 840                 845             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    850                 855                 860                 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
865                 870                 875                 880 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
                885                 890                 895     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            900                 905                 910         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        915                 920                 925             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    930                 935                 940                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
945                 950                 955                 960 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                965                 970                 975     


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Ala Val Phe Gly 
            980                 985                 990         


Cys Ile Phe Gly Ala Leu Val Ile  Val Thr Val Gly Gly  Phe Ile Phe 
        995                 1000                 1005             


Trp Arg  Lys Lys Arg Lys Asp  Ala Lys Asn Asn Glu  Val Ser Phe 
    1010                 1015                 1020             


Ser Gln  Ile Lys Pro Lys Lys  Ser Lys Leu Ile Arg  Val Glu Asn 
    1025                 1030                 1035             


Phe Glu  Ala Tyr Phe Lys Lys  Gln Gln Ala Asp Ser  Asn Cys Gly 
    1040                 1045                 1050             


Phe Ala  Glu Glu Tyr Glu Asp  Leu Lys Leu Val Gly  Ile Ser Gln 
    1055                 1060                 1065             


Pro Lys  Tyr Ala Ala Glu Leu  Ala Glu Asn Arg Gly  Lys Asn Arg 
    1070                 1075                 1080             


Tyr Asn  Asn Val Leu Pro Tyr  Asp Ile Ser Arg Val  Lys Leu Ser 
    1085                 1090                 1095             


Val Gln  Thr His Ser Thr Asp  Asp Tyr Ile Asn Ala  Asn Tyr Met 
    1100                 1105                 1110             


Pro Gly  Tyr His Ser Lys Lys  Asp Phe Ile Ala Thr  Gln Gly Pro 
    1115                 1120                 1125             


Leu Pro  Asn Thr Leu Lys Asp  Phe Trp Arg Met Val  Trp Glu Lys 
    1130                 1135                 1140             


Asn Val  Tyr Ala Ile Ile Met  Leu Thr Lys Cys Val  Glu Gln Gly 
    1145                 1150                 1155             


Arg Thr  Lys Cys Glu Glu Tyr  Trp Pro Ser Lys Gln  Ala Gln Asp 
    1160                 1165                 1170             


Tyr Gly  Asp Ile Thr Val Ala  Met Thr Ser Glu Ile  Val Leu Pro 
    1175                 1180                 1185             


Glu Trp  Thr Ile Arg Asp Phe  Thr Val Lys Asn Ile  Gln Thr Ser 
    1190                 1195                 1200             


Glu Ser  His Pro Leu Arg Gln  Phe His Phe Thr Ser  Trp Pro Asp 
    1205                 1210                 1215             


His Gly  Val Pro Asp Thr Thr  Asp Leu Leu Ile Asn  Phe Arg Tyr 
    1220                 1225                 1230             


Leu Val  Arg Asp Tyr Met Lys  Gln Ser Pro Pro Glu  Ser Pro Ile 
    1235                 1240                 1245             


Leu Val  His Cys Ser Ala Gly  Val Gly Arg Thr Gly  Thr Phe Ile 
    1250                 1255                 1260             


Ala Ile  Asp Arg Leu Ile Tyr  Gln Ile Glu Asn Glu  Asn Thr Val 
    1265                 1270                 1275             


Asp Val  Tyr Gly Ile Val Tyr  Asp Leu Arg Met His  Arg Pro Leu 
    1280                 1285                 1290             


Met Val  Gln Thr Glu Asp Gln  Tyr Val Phe Leu Asn  Gln Cys Val 
    1295                 1300                 1305             


Leu Asp  Ile Val Arg Ser Gln  Lys Asp Ser Lys Val  Asp Leu Ile 
    1310                 1315                 1320             


Tyr Gln  Asn Thr Thr Ala Met  Thr Ile Tyr Glu Asn  Leu Ala Pro 
    1325                 1330                 1335             


Val Thr  Thr Phe Gly Lys Thr  Asn Gly Tyr Ile Ala  
    1340                 1345                 1350 


<210>  3
<211>  1717
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric antigen receptor (CAR)

<400>  3

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        755                 760                 765             


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    770                 775                 780                 


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
785                 790                 795                 800 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                805                 810                 815     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            820                 825                 830         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        835                 840                 845             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    850                 855                 860                 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
865                 870                 875                 880 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
                885                 890                 895     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            900                 905                 910         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        915                 920                 925             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    930                 935                 940                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
945                 950                 955                 960 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                965                 970                 975     


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Ala Leu Ile Ala 
            980                 985                 990         


Phe Leu Ala Phe Leu Ile Ile Val  Thr Ser Ile Ala Leu  Leu Val Val 
        995                 1000                 1005             


Leu Tyr  Lys Ile Tyr Asp Leu  His Lys Lys Arg Ser  Cys Asn Leu 
    1010                 1015                 1020             


Asp Glu  Gln Gln Glu Leu Val  Glu Arg Asp Asp Glu  Lys Gln Leu 
    1025                 1030                 1035             


Met Asn  Val Glu Pro Ile His  Ala Asp Ile Leu Leu  Glu Thr Tyr 
    1040                 1045                 1050             


Lys Arg  Lys Ile Ala Asp Glu  Gly Arg Leu Phe Leu  Ala Glu Phe 
    1055                 1060                 1065             


Gln Ser  Ile Pro Arg Val Phe  Ser Lys Phe Pro Ile  Lys Glu Ala 
    1070                 1075                 1080             


Arg Lys  Pro Phe Asn Gln Asn  Lys Asn Arg Tyr Val  Asp Ile Leu 
    1085                 1090                 1095             


Pro Tyr  Asp Tyr Asn Arg Val  Glu Leu Ser Glu Ile  Asn Gly Asp 
    1100                 1105                 1110             


Ala Gly  Ser Asn Tyr Ile Asn  Ala Ser Tyr Ile Asp  Gly Phe Lys 
    1115                 1120                 1125             


Glu Pro  Arg Lys Tyr Ile Ala  Ala Gln Gly Pro Arg  Asp Glu Thr 
    1130                 1135                 1140             


Val Asp  Asp Phe Trp Arg Met  Ile Trp Glu Gln Lys  Ala Thr Val 
    1145                 1150                 1155             


Ile Val  Met Val Thr Arg Cys  Glu Glu Gly Asn Arg  Asn Lys Cys 
    1160                 1165                 1170             


Ala Glu  Tyr Trp Pro Ser Met  Glu Glu Gly Thr Arg  Ala Phe Gly 
    1175                 1180                 1185             


Asp Val  Val Val Lys Ile Asn  Gln His Lys Arg Cys  Pro Asp Tyr 
    1190                 1195                 1200             


Ile Ile  Gln Lys Leu Asn Ile  Val Asn Lys Lys Glu  Lys Ala Thr 
    1205                 1210                 1215             


Gly Arg  Glu Val Thr His Ile  Gln Phe Thr Ser Trp  Pro Asp His 
    1220                 1225                 1230             


Gly Val  Pro Glu Asp Pro His  Leu Leu Leu Lys Leu  Arg Arg Arg 
    1235                 1240                 1245             


Val Asn  Ala Phe Ser Asn Phe  Phe Ser Gly Pro Ile  Val Val His 
    1250                 1255                 1260             


Cys Ser  Ala Gly Val Gly Arg  Thr Gly Thr Tyr Ile  Gly Ile Asp 
    1265                 1270                 1275             


Ala Met  Leu Glu Gly Leu Glu  Ala Glu Asn Lys Val  Asp Val Tyr 
    1280                 1285                 1290             


Gly Tyr  Val Val Lys Leu Arg  Arg Gln Arg Cys Leu  Met Val Gln 
    1295                 1300                 1305             


Val Glu  Ala Gln Tyr Ile Leu  Ile His Gln Ala Leu  Val Glu Tyr 
    1310                 1315                 1320             


Asn Gln  Phe Gly Glu Thr Glu  Val Asn Leu Ser Glu  Leu His Pro 
    1325                 1330                 1335             


Tyr Leu  His Asn Met Lys Lys  Arg Asp Pro Pro Ser  Glu Pro Ser 
    1340                 1345                 1350             


Pro Leu  Glu Ala Glu Phe Gln  Arg Leu Pro Ser Tyr  Arg Ser Trp 
    1355                 1360                 1365             


Arg Thr  Gln His Ile Gly Asn  Gln Glu Glu Asn Lys  Ser Lys Asn 
    1370                 1375                 1380             


Arg Asn  Ser Asn Val Ile Pro  Tyr Asp Tyr Asn Arg  Val Pro Leu 
    1385                 1390                 1395             


Lys His  Glu Leu Glu Met Ser  Lys Glu Ser Glu His  Asp Ser Asp 
    1400                 1405                 1410             


Glu Ser  Ser Asp Asp Asp Ser  Asp Ser Glu Glu Pro  Ser Lys Tyr 
    1415                 1420                 1425             


Ile Asn  Ala Ser Phe Ile Met  Ser Tyr Trp Lys Pro  Glu Val Met 
    1430                 1435                 1440             


Ile Ala  Ala Gln Gly Pro Leu  Lys Glu Thr Ile Gly  Asp Phe Trp 
    1445                 1450                 1455             


Gln Met  Ile Phe Gln Arg Lys  Val Lys Val Ile Val  Met Leu Thr 
    1460                 1465                 1470             


Glu Leu  Lys His Gly Asp Gln  Glu Ile Cys Ala Gln  Tyr Trp Gly 
    1475                 1480                 1485             


Glu Gly  Lys Gln Thr Tyr Gly  Asp Ile Glu Val Asp  Leu Lys Asp 
    1490                 1495                 1500             


Thr Asp  Lys Ser Ser Thr Tyr  Thr Leu Arg Val Phe  Glu Leu Arg 
    1505                 1510                 1515             


His Ser  Lys Arg Lys Asp Ser  Arg Thr Val Tyr Gln  Tyr Gln Tyr 
    1520                 1525                 1530             


Thr Asn  Trp Ser Val Glu Gln  Leu Pro Ala Glu Pro  Lys Glu Leu 
    1535                 1540                 1545             


Ile Ser  Met Ile Gln Val Val  Lys Gln Lys Leu Pro  Gln Lys Asn 
    1550                 1555                 1560             


Ser Ser  Glu Gly Asn Lys His  His Lys Ser Thr Pro  Leu Leu Ile 
    1565                 1570                 1575             


His Cys  Arg Asp Gly Ser Gln  Gln Thr Gly Ile Phe  Cys Ala Leu 
    1580                 1585                 1590             


Leu Asn  Leu Leu Glu Ser Ala  Glu Thr Glu Glu Val  Val Asp Ile 
    1595                 1600                 1605             


Phe Gln  Val Val Lys Ala Leu  Arg Lys Ala Arg Pro  Gly Met Val 
    1610                 1615                 1620             


Ser Thr  Phe Glu Gln Tyr Gln  Phe Leu Tyr Asp Val  Ile Ala Ser 
    1625                 1630                 1635             


Thr Tyr  Pro Ala Gln Asn Gly  Gln Val Lys Lys Asn  Asn His Gln 
    1640                 1645                 1650             


Glu Asp  Lys Ile Glu Phe Asp  Asn Glu Val Asp Lys  Val Lys Gln 
    1655                 1660                 1665             


Asp Ala  Asn Cys Val Asn Pro  Leu Gly Ala Pro Glu  Lys Leu Pro 
    1670                 1675                 1680             


Glu Ala  Lys Glu Gln Ala Glu  Gly Ser Glu Pro Thr  Ser Gly Thr 
    1685                 1690                 1695             


Glu Gly  Pro Glu His Ser Val  Asn Gly Pro Ala Ser  Pro Ala Leu 
    1700                 1705                 1710             


Asn Gln  Gly Ser 
    1715         


<210>  4
<211>  1114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric antigen receptor (CAR)

<400>  4

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro 
        755                 760                 765             


Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly 
    770                 775                 780                 


Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Tyr Trp Ala 
785                 790                 795                 800 


Pro Leu Ala Gly Ile Cys Val Ala Leu Leu Leu Ser Leu Ile Ile Thr 
                805                 810                 815     


Leu Ile Cys Tyr His Arg Ser Arg Lys Arg Val Cys Lys Ser Gly Gly 
            820                 825                 830         


Gly Ser Phe Trp Glu Glu Phe Glu Ser Leu Gln Lys Gln Glu Val Lys 
        835                 840                 845             


Asn Leu His Gln Arg Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly Lys 
    850                 855                 860                 


Asn Arg Tyr Lys Asn Ile Leu Pro Phe Asp His Ser Arg Val Ile Leu 
865                 870                 875                 880 


Gln Gly Arg Asp Ser Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala Asn 
                885                 890                 895     


Tyr Ile Lys Asn Gln Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr Tyr 
            900                 905                 910         


Ile Ala Ser Gln Gly Cys Leu Glu Ala Thr Val Asn Asp Phe Trp Gln 
        915                 920                 925             


Met Ala Trp Gln Glu Asn Ser Arg Val Ile Val Met Thr Thr Arg Glu 
    930                 935                 940                 


Val Glu Lys Gly Arg Asn Lys Cys Val Pro Tyr Trp Pro Glu Val Gly 
945                 950                 955                 960 


Met Gln Arg Ala Tyr Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu His 
                965                 970                 975     


Asp Thr Thr Glu Tyr Lys Leu Arg Thr Leu Gln Val Ser Pro Leu Asp 
            980                 985                 990         


Asn Gly Asp Leu Ile Arg Glu Ile  Trp His Tyr Gln Tyr  Leu Ser Trp 
        995                 1000                 1005             


Pro Asp  His Gly Val Pro Ser  Glu Pro Gly Gly Val  Leu Ser Phe 
    1010                 1015                 1020             


Leu Asp  Gln Ile Asn Gln Arg  Gln Glu Ser Leu Pro  His Ala Gly 
    1025                 1030                 1035             


Pro Ile  Ile Val His Cys Ser  Ala Gly Ile Gly Arg  Thr Gly Thr 
    1040                 1045                 1050             


Ile Ile  Val Ile Asp Met Leu  Met Glu Asn Ile Ser  Thr Lys Gly 
    1055                 1060                 1065             


Leu Asp  Cys Asp Ile Asp Ile  Gln Lys Thr Ile Gln  Met Val Arg 
    1070                 1075                 1080             


Ala Gln  Arg Ser Gly Met Val  Gln Thr Glu Ala Gln  Tyr Lys Phe 
    1085                 1090                 1095             


Ile Tyr  Val Ala Ile Ala Gln  Phe Ile Glu Thr Thr  Lys Lys Lys 
    1100                 1105                 1110             


Leu 
    


<210>  5
<211>  918
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric antigen receptor (CAR)

<400>  5

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro 
        755                 760                 765             


Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly 
    770                 775                 780                 


Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Leu Ile Gly 
785                 790                 795                 800 


Val Ser Val Val Phe Leu Phe Cys Leu Leu Leu Leu Val Leu Phe Cys 
                805                 810                 815     


Leu His Arg Gln Asn Gln Ile Lys Gln Gly Pro Pro Arg Ser Lys Asp 
            820                 825                 830         


Glu Glu Gln Lys Pro Gln Gln Arg Pro Asp Leu Ala Val Asp Val Leu 
        835                 840                 845             


Glu Arg Thr Ala Asp Lys Ala Thr Val Asn Gly Leu Pro Glu Lys Asp 
    850                 855                 860                 


Arg Glu Thr Asp Thr Ser Ala Leu Ala Ala Gly Ser Ser Gln Glu Val 
865                 870                 875                 880 


Thr Tyr Ala Gln Leu Asp His Trp Ala Leu Thr Gln Arg Thr Ala Arg 
                885                 890                 895     


Ala Val Ser Pro Gln Ser Thr Lys Pro Met Ala Glu Ser Ile Thr Tyr 
            900                 905                 910         


Ala Ala Val Ala Arg His 
        915             


<210>  6
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric antigen receptor (CAR)

<400>  6

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro 
        755                 760                 765             


Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly 
    770                 775                 780                 


Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Leu Ile Gly 
785                 790                 795                 800 


Val Ser Val Val Phe Leu Phe Cys Leu Leu Leu Leu Val Leu Phe Cys 
                805                 810                 815     


Leu His Arg Gln Asn Gln Ile Lys Gln Gly Pro Pro Arg Ser Lys Asp 
            820                 825                 830         


Glu Glu Gln Lys Pro Gln Gln Arg Pro Asp Leu Ala Val Asp Val Leu 
        835                 840                 845             


Glu Arg Thr Ala Asp Lys Ala Thr Val Asn Gly Leu Pro Glu Lys Asp 
    850                 855                 860                 


Arg Glu Thr Asp Thr Ser Ala Leu Ala Ala Gly Ser Ser Gln Glu Val 
865                 870                 875                 880 


Thr Tyr Ala Gln Leu Asp His Trp Ala Leu Thr Gln Arg Thr Ala Arg 
                885                 890                 895     


Ala Val Ser Pro Gln Ser Thr Lys Pro Met Ala Glu Ser Ile Thr Tyr 
            900                 905                 910         


Ala Ala Val Ala Arg His Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr 
        915                 920                 925             


Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Trp Tyr His Gly His Met 
    930                 935                 940                 


Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly Glu Pro Trp 
945                 950                 955                 960 


Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp Phe Val Leu 
                965                 970                 975     


Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser Pro Leu Arg 
            980                 985                 990         


Val Thr His Ile Lys Val Met Cys  Glu Gly Gly Arg Tyr  Thr Val Gly 
        995                 1000                 1005             


Gly Leu  Glu Thr Phe Asp Ser  Leu Thr Asp Leu Val  Glu His Phe 
    1010                 1015                 1020             


Lys Lys  Thr Gly Ile Glu Glu  Ala Ser Gly Ala Phe  Val Tyr Leu 
    1025                 1030                 1035             


Arg Gln  Pro Tyr Ser Gly Gly  Gly Gly Ser Phe Glu  Ala Tyr Phe 
    1040                 1045                 1050             


Lys Lys  Gln Gln Ala Asp Ser  Asn Cys Gly Phe Ala  Glu Glu Tyr 
    1055                 1060                 1065             


Glu Asp  Leu Lys Leu Val Gly  Ile Ser Gln Pro Lys  Tyr Ala Ala 
    1070                 1075                 1080             


Glu Leu  Ala Glu Asn Arg Gly  Lys Asn Arg Tyr Asn  Asn Val Leu 
    1085                 1090                 1095             


Pro Tyr  Asp Ile Ser Arg Val  Lys Leu Ser Val Gln  Thr His Ser 
    1100                 1105                 1110             


Thr Asp  Asp Tyr Ile Asn Ala  Asn Tyr Met Pro Gly  Tyr His Ser 
    1115                 1120                 1125             


Lys Lys  Asp Phe Ile Ala Thr  Gln Gly Pro Leu Pro  Asn Thr Leu 
    1130                 1135                 1140             


Lys Asp  Phe Trp Arg Met Val  Trp Glu Lys Asn Val  Tyr Ala Ile 
    1145                 1150                 1155             


Ile Met  Leu Thr Lys Cys Val  Glu Gln Gly Arg Thr  Lys Cys Glu 
    1160                 1165                 1170             


Glu Tyr  Trp Pro Ser Lys Gln  Ala Gln Asp Tyr Gly  Asp Ile Thr 
    1175                 1180                 1185             


Val Ala  Met Thr Ser Glu Ile  Val Leu Pro Glu Trp  Thr Ile Arg 
    1190                 1195                 1200             


Asp Phe  Thr Val Lys Asn Ile  Gln Thr Ser Glu Ser  His Pro Leu 
    1205                 1210                 1215             


Arg Gln  Phe His Phe Thr Ser  Trp Pro Asp His Gly  Val Pro Asp 
    1220                 1225                 1230             


Thr Thr  Asp Leu Leu Ile Asn  Phe Arg Tyr Leu Val  Arg Asp Tyr 
    1235                 1240                 1245             


Met Lys  Gln Ser Pro Pro Glu  Ser Pro Ile Leu Val  His Cys Ser 
    1250                 1255                 1260             


Ala Gly  Val Gly Arg Thr Gly  Thr Phe Ile Ala Ile  Asp Arg Leu 
    1265                 1270                 1275             


Ile Tyr  Gln Ile Glu Asn Glu  Asn Thr Val Asp Val  Tyr Gly Ile 
    1280                 1285                 1290             


Val Tyr  Asp Leu Arg Met His  Arg Pro Leu Met Val  Gln Thr Glu 
    1295                 1300                 1305             


Asp Gln  Tyr Val Phe Leu Asn  Gln Cys Val Leu Asp  Ile Val Arg 
    1310                 1315                 1320             


Ser Gln  Lys Asp Ser Lys Val  Asp Leu Ile Tyr Gln  Asn Thr Thr 
    1325                 1330                 1335             


Ala Met  Thr Ile Tyr Glu Asn  Leu Ala Pro Val Thr  Thr Phe Gly 
    1340                 1345                 1350             


Lys Thr  Asn Gly Tyr Ile Ala  Ser Gly Ser 
    1355                 1360             


<210>  7
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  7

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Gly 
            20      


<210>  8
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  8

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  9
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  9

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys 
            20  


<210>  10
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer (hinge-CH2CH3 of human IgG1)

<400>  10

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  11
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer (human CD8 stalk)

<400>  11

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  12
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer (human IgG1 hinge)

<400>  12

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  13
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer (CD2 ectodomain)

<400>  13

Lys Glu Ile Thr Asn Ala Leu Glu Thr Trp Gly Ala Leu Gly Gln Asp 
1               5                   10                  15      


Ile Asn Leu Asp Ile Pro Ser Phe Gln Met Ser Asp Asp Ile Asp Asp 
            20                  25                  30          


Ile Lys Trp Glu Lys Thr Ser Asp Lys Lys Lys Ile Ala Gln Phe Arg 
        35                  40                  45              


Lys Glu Lys Glu Thr Phe Lys Glu Lys Asp Thr Tyr Lys Leu Phe Lys 
    50                  55                  60                  


Asn Gly Thr Leu Lys Ile Lys His Leu Lys Thr Asp Asp Gln Asp Ile 
65                  70                  75                  80  


Tyr Lys Val Ser Ile Tyr Asp Thr Lys Gly Lys Asn Val Leu Glu Lys 
                85                  90                  95      


Ile Phe Asp Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser 
            100                 105                 110         


Trp Thr Cys Ile Asn Thr Thr Leu Thr Cys Glu Val Met Asn Gly Thr 
        115                 120                 125             


Asp Pro Glu Leu Asn Leu Tyr Gln Asp Gly Lys His Leu Lys Leu Ser 
    130                 135                 140                 


Gln Arg Val Ile Thr His Lys Trp Thr Thr Ser Leu Ser Ala Lys Phe 
145                 150                 155                 160 


Lys Cys Thr Ala Gly Asn Lys Val Ser Lys Glu Ser Ser Val Glu Pro 
                165                 170                 175     


Val Ser Cys Pro Glu Lys Gly Leu Asp 
            180                 185 


<210>  14
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer (CD34 ectodomain)

<400>  14

Ser Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly 
1               5                   10                  15      


Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr 
            20                  25                  30          


Pro Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly 
        35                  40                  45              


Asn Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser 
    50                  55                  60                  


Thr Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln 
65                  70                  75                  80  


Ser Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val 
                85                  90                  95      


Ser Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val 
            100                 105                 110         


Ser Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys 
        115                 120                 125             


Pro Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile 
    130                 135                 140                 


Lys Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu 
145                 150                 155                 160 


Glu Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly 
                165                 170                 175     


Glu Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp 
            180                 185                 190         


Ala Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg 
        195                 200                 205             


Pro Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser 
    210                 215                 220                 


Lys Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly 
225                 230                 235                 240 


Ile Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser 
                245                 250                 255     


Gln Lys Thr 
            


<210>  15
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD3 Z endodomains

<400>  15

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe 
            20                  25                  30          


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
        35                  40                  45              


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
    50                  55                  60                  


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
65                  70                  75                  80  


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                85                  90                  95      


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            100                 105                 110         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        115                 120                 125             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    130                 135                 140 


<210>  16
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD28 and CD3 Zeta endodomains

<400>  16

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
65                  70                  75                  80  


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                85                  90                  95      


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            100                 105                 110         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        115                 120                 125             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    130                 135                 140                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
145                 150                 155                 160 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                165                 170                 175     


Leu Pro Pro Arg 
            180 


<210>  17
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD28, OX40 and CD3 Zeta endodomains

<400>  17

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro 
65                  70                  75                  80  


Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp 
                85                  90                  95      


Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala 
            100                 105                 110         


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
        115                 120                 125             


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
    130                 135                 140                 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
145                 150                 155                 160 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                165                 170                 175     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            180                 185                 190         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        195                 200                 205             


His Met Gln Ala Leu Pro Pro Arg 
    210                 215     


<210>  18
<211>  729
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD45 transmembrane and endodomain

<400>  18

Ala Leu Ile Ala Phe Leu Ala Phe Leu Ile Ile Val Thr Ser Ile Ala 
1               5                   10                  15      


Leu Leu Val Val Leu Tyr Lys Ile Tyr Asp Leu His Lys Lys Arg Ser 
            20                  25                  30          


Cys Asn Leu Asp Glu Gln Gln Glu Leu Val Glu Arg Asp Asp Glu Lys 
        35                  40                  45              


Gln Leu Met Asn Val Glu Pro Ile His Ala Asp Ile Leu Leu Glu Thr 
    50                  55                  60                  


Tyr Lys Arg Lys Ile Ala Asp Glu Gly Arg Leu Phe Leu Ala Glu Phe 
65                  70                  75                  80  


Gln Ser Ile Pro Arg Val Phe Ser Lys Phe Pro Ile Lys Glu Ala Arg 
                85                  90                  95      


Lys Pro Phe Asn Gln Asn Lys Asn Arg Tyr Val Asp Ile Leu Pro Tyr 
            100                 105                 110         


Asp Tyr Asn Arg Val Glu Leu Ser Glu Ile Asn Gly Asp Ala Gly Ser 
        115                 120                 125             


Asn Tyr Ile Asn Ala Ser Tyr Ile Asp Gly Phe Lys Glu Pro Arg Lys 
    130                 135                 140                 


Tyr Ile Ala Ala Gln Gly Pro Arg Asp Glu Thr Val Asp Asp Phe Trp 
145                 150                 155                 160 


Arg Met Ile Trp Glu Gln Lys Ala Thr Val Ile Val Met Val Thr Arg 
                165                 170                 175     


Cys Glu Glu Gly Asn Arg Asn Lys Cys Ala Glu Tyr Trp Pro Ser Met 
            180                 185                 190         


Glu Glu Gly Thr Arg Ala Phe Gly Asp Val Val Val Lys Ile Asn Gln 
        195                 200                 205             


His Lys Arg Cys Pro Asp Tyr Ile Ile Gln Lys Leu Asn Ile Val Asn 
    210                 215                 220                 


Lys Lys Glu Lys Ala Thr Gly Arg Glu Val Thr His Ile Gln Phe Thr 
225                 230                 235                 240 


Ser Trp Pro Asp His Gly Val Pro Glu Asp Pro His Leu Leu Leu Lys 
                245                 250                 255     


Leu Arg Arg Arg Val Asn Ala Phe Ser Asn Phe Phe Ser Gly Pro Ile 
            260                 265                 270         


Val Val His Cys Ser Ala Gly Val Gly Arg Thr Gly Thr Tyr Ile Gly 
        275                 280                 285             


Ile Asp Ala Met Leu Glu Gly Leu Glu Ala Glu Asn Lys Val Asp Val 
    290                 295                 300                 


Tyr Gly Tyr Val Val Lys Leu Arg Arg Gln Arg Cys Leu Met Val Gln 
305                 310                 315                 320 


Val Glu Ala Gln Tyr Ile Leu Ile His Gln Ala Leu Val Glu Tyr Asn 
                325                 330                 335     


Gln Phe Gly Glu Thr Glu Val Asn Leu Ser Glu Leu His Pro Tyr Leu 
            340                 345                 350         


His Asn Met Lys Lys Arg Asp Pro Pro Ser Glu Pro Ser Pro Leu Glu 
        355                 360                 365             


Ala Glu Phe Gln Arg Leu Pro Ser Tyr Arg Ser Trp Arg Thr Gln His 
    370                 375                 380                 


Ile Gly Asn Gln Glu Glu Asn Lys Ser Lys Asn Arg Asn Ser Asn Val 
385                 390                 395                 400 


Ile Pro Tyr Asp Tyr Asn Arg Val Pro Leu Lys His Glu Leu Glu Met 
                405                 410                 415     


Ser Lys Glu Ser Glu His Asp Ser Asp Glu Ser Ser Asp Asp Asp Ser 
            420                 425                 430         


Asp Ser Glu Glu Pro Ser Lys Tyr Ile Asn Ala Ser Phe Ile Met Ser 
        435                 440                 445             


Tyr Trp Lys Pro Glu Val Met Ile Ala Ala Gln Gly Pro Leu Lys Glu 
    450                 455                 460                 


Thr Ile Gly Asp Phe Trp Gln Met Ile Phe Gln Arg Lys Val Lys Val 
465                 470                 475                 480 


Ile Val Met Leu Thr Glu Leu Lys His Gly Asp Gln Glu Ile Cys Ala 
                485                 490                 495     


Gln Tyr Trp Gly Glu Gly Lys Gln Thr Tyr Gly Asp Ile Glu Val Asp 
            500                 505                 510         


Leu Lys Asp Thr Asp Lys Ser Ser Thr Tyr Thr Leu Arg Val Phe Glu 
        515                 520                 525             


Leu Arg His Ser Lys Arg Lys Asp Ser Arg Thr Val Tyr Gln Tyr Gln 
    530                 535                 540                 


Tyr Thr Asn Trp Ser Val Glu Gln Leu Pro Ala Glu Pro Lys Glu Leu 
545                 550                 555                 560 


Ile Ser Met Ile Gln Val Val Lys Gln Lys Leu Pro Gln Lys Asn Ser 
                565                 570                 575     


Ser Glu Gly Asn Lys His His Lys Ser Thr Pro Leu Leu Ile His Cys 
            580                 585                 590         


Arg Asp Gly Ser Gln Gln Thr Gly Ile Phe Cys Ala Leu Leu Asn Leu 
        595                 600                 605             


Leu Glu Ser Ala Glu Thr Glu Glu Val Val Asp Ile Phe Gln Val Val 
    610                 615                 620                 


Lys Ala Leu Arg Lys Ala Arg Pro Gly Met Val Ser Thr Phe Glu Gln 
625                 630                 635                 640 


Tyr Gln Phe Leu Tyr Asp Val Ile Ala Ser Thr Tyr Pro Ala Gln Asn 
                645                 650                 655     


Gly Gln Val Lys Lys Asn Asn His Gln Glu Asp Lys Ile Glu Phe Asp 
            660                 665                 670         


Asn Glu Val Asp Lys Val Lys Gln Asp Ala Asn Cys Val Asn Pro Leu 
        675                 680                 685             


Gly Ala Pro Glu Lys Leu Pro Glu Ala Lys Glu Gln Ala Glu Gly Ser 
    690                 695                 700                 


Glu Pro Thr Ser Gly Thr Glu Gly Pro Glu His Ser Val Asn Gly Pro 
705                 710                 715                 720 


Ala Ser Pro Ala Leu Asn Gln Gly Ser 
                725                 


<210>  19
<211>  362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD148 transmembrane and endodomain

<400>  19

Ala Val Phe Gly Cys Ile Phe Gly Ala Leu Val Ile Val Thr Val Gly 
1               5                   10                  15      


Gly Phe Ile Phe Trp Arg Lys Lys Arg Lys Asp Ala Lys Asn Asn Glu 
            20                  25                  30          


Val Ser Phe Ser Gln Ile Lys Pro Lys Lys Ser Lys Leu Ile Arg Val 
        35                  40                  45              


Glu Asn Phe Glu Ala Tyr Phe Lys Lys Gln Gln Ala Asp Ser Asn Cys 
    50                  55                  60                  


Gly Phe Ala Glu Glu Tyr Glu Asp Leu Lys Leu Val Gly Ile Ser Gln 
65                  70                  75                  80  


Pro Lys Tyr Ala Ala Glu Leu Ala Glu Asn Arg Gly Lys Asn Arg Tyr 
                85                  90                  95      


Asn Asn Val Leu Pro Tyr Asp Ile Ser Arg Val Lys Leu Ser Val Gln 
            100                 105                 110         


Thr His Ser Thr Asp Asp Tyr Ile Asn Ala Asn Tyr Met Pro Gly Tyr 
        115                 120                 125             


His Ser Lys Lys Asp Phe Ile Ala Thr Gln Gly Pro Leu Pro Asn Thr 
    130                 135                 140                 


Leu Lys Asp Phe Trp Arg Met Val Trp Glu Lys Asn Val Tyr Ala Ile 
145                 150                 155                 160 


Ile Met Leu Thr Lys Cys Val Glu Gln Gly Arg Thr Lys Cys Glu Glu 
                165                 170                 175     


Tyr Trp Pro Ser Lys Gln Ala Gln Asp Tyr Gly Asp Ile Thr Val Ala 
            180                 185                 190         


Met Thr Ser Glu Ile Val Leu Pro Glu Trp Thr Ile Arg Asp Phe Thr 
        195                 200                 205             


Val Lys Asn Ile Gln Thr Ser Glu Ser His Pro Leu Arg Gln Phe His 
    210                 215                 220                 


Phe Thr Ser Trp Pro Asp His Gly Val Pro Asp Thr Thr Asp Leu Leu 
225                 230                 235                 240 


Ile Asn Phe Arg Tyr Leu Val Arg Asp Tyr Met Lys Gln Ser Pro Pro 
                245                 250                 255     


Glu Ser Pro Ile Leu Val His Cys Ser Ala Gly Val Gly Arg Thr Gly 
            260                 265                 270         


Thr Phe Ile Ala Ile Asp Arg Leu Ile Tyr Gln Ile Glu Asn Glu Asn 
        275                 280                 285             


Thr Val Asp Val Tyr Gly Ile Val Tyr Asp Leu Arg Met His Arg Pro 
    290                 295                 300                 


Leu Met Val Gln Thr Glu Asp Gln Tyr Val Phe Leu Asn Gln Cys Val 
305                 310                 315                 320 


Leu Asp Ile Val Arg Ser Gln Lys Asp Ser Lys Val Asp Leu Ile Tyr 
                325                 330                 335     


Gln Asn Thr Thr Ala Met Thr Ile Tyr Glu Asn Leu Ala Pro Val Thr 
            340                 345                 350         


Thr Phe Gly Lys Thr Asn Gly Tyr Ile Ala 
        355                 360         


<210>  20
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of PTPN6

<400>  20

Met Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr 
1               5                   10                  15      


Leu Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser 
            20                  25                  30          


Arg Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln 
        35                  40                  45              


Val Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr 
    50                  55                  60                  


Gly Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr 
65                  70                  75                  80  


Gln Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu 
                85                  90                  95      


Lys Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His 
            100                 105                 110         


Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly 
        115                 120                 125             


Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp 
    130                 135                 140                 


Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser 
145                 150                 155                 160 


Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr 
                165                 170                 175     


Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu 
            180                 185                 190         


His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr 
        195                 200                 205             


Leu Arg Gln Pro Tyr Tyr Ala Thr Arg Val Asn Ala Ala Asp Ile Glu 
    210                 215                 220                 


Asn Arg Val Leu Glu Leu Asn Lys Lys Gln Glu Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Lys Ala Gly Phe Trp Glu Glu Phe Glu Ser Leu Gln Lys Gln Glu Val 
                245                 250                 255     


Lys Asn Leu His Gln Arg Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly 
            260                 265                 270         


Lys Asn Arg Tyr Lys Asn Ile Leu Pro Phe Asp His Ser Arg Val Ile 
        275                 280                 285             


Leu Gln Gly Arg Asp Ser Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala 
    290                 295                 300                 


Asn Tyr Ile Lys Asn Gln Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr 
305                 310                 315                 320 


Tyr Ile Ala Ser Gln Gly Cys Leu Glu Ala Thr Val Asn Asp Phe Trp 
                325                 330                 335     


Gln Met Ala Trp Gln Glu Asn Ser Arg Val Ile Val Met Thr Thr Arg 
            340                 345                 350         


Glu Val Glu Lys Gly Arg Asn Lys Cys Val Pro Tyr Trp Pro Glu Val 
        355                 360                 365             


Gly Met Gln Arg Ala Tyr Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu 
    370                 375                 380                 


His Asp Thr Thr Glu Tyr Lys Leu Arg Thr Leu Gln Val Ser Pro Leu 
385                 390                 395                 400 


Asp Asn Gly Asp Leu Ile Arg Glu Ile Trp His Tyr Gln Tyr Leu Ser 
                405                 410                 415     


Trp Pro Asp His Gly Val Pro Ser Glu Pro Gly Gly Val Leu Ser Phe 
            420                 425                 430         


Leu Asp Gln Ile Asn Gln Arg Gln Glu Ser Leu Pro His Ala Gly Pro 
        435                 440                 445             


Ile Ile Val His Cys Ser Ala Gly Ile Gly Arg Thr Gly Thr Ile Ile 
    450                 455                 460                 


Val Ile Asp Met Leu Met Glu Asn Ile Ser Thr Lys Gly Leu Asp Cys 
465                 470                 475                 480 


Asp Ile Asp Ile Gln Lys Thr Ile Gln Met Val Arg Ala Gln Arg Ser 
                485                 490                 495     


Gly Met Val Gln Thr Glu Ala Gln Tyr Lys Phe Ile Tyr Val Ala Ile 
            500                 505                 510         


Ala Gln Phe Ile Glu Thr Thr Lys Lys Lys Leu Glu Val Leu Gln Ser 
        515                 520                 525             


Gln Lys Gly Gln Glu Ser Glu Tyr Gly Asn Ile Thr Tyr Pro Pro Ala 
    530                 535                 540                 


Met Lys Asn Ala His Ala Lys Ala Ser Arg Thr Ser Ser Lys His Lys 
545                 550                 555                 560 


Glu Asp Val Tyr Glu Asn Leu His Thr Lys Asn Lys Arg Glu Glu Lys 
                565                 570                 575     


Val Lys Lys Gln Arg Ser Ala Asp Lys Glu Lys Ser Lys Gly Ser Leu 
            580                 585                 590         


Lys Arg Lys 
        595 


<210>  21
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of phosphatase domain of PTPN6

<400>  21

Phe Trp Glu Glu Phe Glu Ser Leu Gln Lys Gln Glu Val Lys Asn Leu 
1               5                   10                  15      


His Gln Arg Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly Lys Asn Arg 
            20                  25                  30          


Tyr Lys Asn Ile Leu Pro Phe Asp His Ser Arg Val Ile Leu Gln Gly 
        35                  40                  45              


Arg Asp Ser Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala Asn Tyr Ile 
    50                  55                  60                  


Lys Asn Gln Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr Tyr Ile Ala 
65                  70                  75                  80  


Ser Gln Gly Cys Leu Glu Ala Thr Val Asn Asp Phe Trp Gln Met Ala 
                85                  90                  95      


Trp Gln Glu Asn Ser Arg Val Ile Val Met Thr Thr Arg Glu Val Glu 
            100                 105                 110         


Lys Gly Arg Asn Lys Cys Val Pro Tyr Trp Pro Glu Val Gly Met Gln 
        115                 120                 125             


Arg Ala Tyr Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu His Asp Thr 
    130                 135                 140                 


Thr Glu Tyr Lys Leu Arg Thr Leu Gln Val Ser Pro Leu Asp Asn Gly 
145                 150                 155                 160 


Asp Leu Ile Arg Glu Ile Trp His Tyr Gln Tyr Leu Ser Trp Pro Asp 
                165                 170                 175     


His Gly Val Pro Ser Glu Pro Gly Gly Val Leu Ser Phe Leu Asp Gln 
            180                 185                 190         


Ile Asn Gln Arg Gln Glu Ser Leu Pro His Ala Gly Pro Ile Ile Val 
        195                 200                 205             


His Cys Ser Ala Gly Ile Gly Arg Thr Gly Thr Ile Ile Val Ile Asp 
    210                 215                 220                 


Met Leu Met Glu Asn Ile Ser Thr Lys Gly Leu Asp Cys Asp Ile Asp 
225                 230                 235                 240 


Ile Gln Lys Thr Ile Gln Met Val Arg Ala Gln Arg Ser Gly Met Val 
                245                 250                 255     


Gln Thr Glu Ala Gln Tyr Lys Phe Ile Tyr Val Ala Ile Ala Gln Phe 
            260                 265                 270         


<210>  22
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDCD1 endodomain

<400>  22

Cys Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln 
1               5                   10                  15      


Pro Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr 
            20                  25                  30          


Gly Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val 
        35                  40                  45              


Pro Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser 
    50                  55                  60                  


Gly Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro 
65                  70                  75                  80  


Arg Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro 
                85                  90                  95      


Leu 
    


<210>  23
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BTLA4 endodomain

<400>  23

Lys Leu Gln Arg Arg Trp Lys Arg Thr Gln Ser Gln Gln Gly Leu Gln 
1               5                   10                  15      


Glu Asn Ser Ser Gly Gln Ser Phe Phe Val Arg Asn Lys Lys Val Arg 
            20                  25                  30          


Arg Ala Pro Leu Ser Glu Gly Pro His Ser Leu Gly Cys Tyr Asn Pro 
        35                  40                  45              


Met Met Glu Asp Gly Ile Ser Tyr Thr Thr Leu Arg Phe Pro Glu Met 
    50                  55                  60                  


Asn Ile Pro Arg Thr Gly Asp Ala Glu Ser Ser Glu Met Gln Arg Pro 
65                  70                  75                  80  


Pro Pro Asp Cys Asp Asp Thr Val Thr Tyr Ser Ala Leu His Lys Arg 
                85                  90                  95      


Gln Val Gly Asp Tyr Glu Asn Val Ile Pro Asp Phe Pro Glu Asp Glu 
            100                 105                 110         


Gly Ile His Tyr Ser Glu Leu Ile Gln Phe Gly Val Gly Glu Arg Pro 
        115                 120                 125             


Gln Ala Gln Glu Asn Val Asp Tyr Val Ile Leu Lys His 
    130                 135                 140     


<210>  24
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LILRB1 endodomain

<400>  24

Leu Arg His Arg Arg Gln Gly Lys His Trp Thr Ser Thr Gln Arg Lys 
1               5                   10                  15      


Ala Asp Phe Gln His Pro Ala Gly Ala Val Gly Pro Glu Pro Thr Asp 
            20                  25                  30          


Arg Gly Leu Gln Trp Arg Ser Ser Pro Ala Ala Asp Ala Gln Glu Glu 
        35                  40                  45              


Asn Leu Tyr Ala Ala Val Lys His Thr Gln Pro Glu Asp Gly Val Glu 
    50                  55                  60                  


Met Asp Thr Arg Ser Pro His Asp Glu Asp Pro Gln Ala Val Thr Tyr 
65                  70                  75                  80  


Ala Glu Val Lys His Ser Arg Pro Arg Arg Glu Met Ala Ser Pro Pro 
                85                  90                  95      


Ser Pro Leu Ser Gly Glu Phe Leu Asp Thr Lys Asp Arg Gln Ala Glu 
            100                 105                 110         


Glu Asp Arg Gln Met Asp Thr Glu Ala Ala Ala Ser Glu Ala Pro Gln 
        115                 120                 125             


Asp Val Thr Tyr Ala Gln Leu His Ser Leu Thr Leu Arg Arg Glu Ala 
    130                 135                 140                 


Thr Glu Pro Pro Pro Ser Gln Glu Gly Pro Ser Pro Ala Val Pro Ser 
145                 150                 155                 160 


Ile Tyr Ala Thr Leu Ala Ile His 
                165             


<210>  25
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LAIR1 endodomain

<400>  25

His Arg Gln Asn Gln Ile Lys Gln Gly Pro Pro Arg Ser Lys Asp Glu 
1               5                   10                  15      


Glu Gln Lys Pro Gln Gln Arg Pro Asp Leu Ala Val Asp Val Leu Glu 
            20                  25                  30          


Arg Thr Ala Asp Lys Ala Thr Val Asn Gly Leu Pro Glu Lys Asp Arg 
        35                  40                  45              


Glu Thr Asp Thr Ser Ala Leu Ala Ala Gly Ser Ser Gln Glu Val Thr 
    50                  55                  60                  


Tyr Ala Gln Leu Asp His Trp Ala Leu Thr Gln Arg Thr Ala Arg Ala 
65                  70                  75                  80  


Val Ser Pro Gln Ser Thr Lys Pro Met Ala Glu Ser Ile Thr Tyr Ala 
                85                  90                  95      


Ala Val Ala Arg His 
            100     


<210>  26
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTLA4 endodomain

<400>  26

Phe Leu Leu Trp Ile Leu Ala Ala Val Ser Ser Gly Leu Phe Phe Tyr 
1               5                   10                  15      


Ser Phe Leu Leu Thr Ala Val Ser Leu Ser Lys Met Leu Lys Lys Arg 
            20                  25                  30          


Ser Pro Leu Thr Thr Gly Val Tyr Val Lys Met Pro Pro Thr Glu Pro 
        35                  40                  45              


Glu Cys Glu Lys Gln Phe Gln Pro Tyr Phe Ile Pro Ile Asn 
    50                  55                  60          


<210>  27
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR2DL1 endodomain

<400>  27

Gly Asn Ser Arg His Leu His Val Leu Ile Gly Thr Ser Val Val Ile 
1               5                   10                  15      


Ile Pro Phe Ala Ile Leu Leu Phe Phe Leu Leu His Arg Trp Cys Ala 
            20                  25                  30          


Asn Lys Lys Asn Ala Val Val Met Asp Gln Glu Pro Ala Gly Asn Arg 
        35                  40                  45              


Thr Val Asn Arg Glu Asp Ser Asp Glu Gln Asp Pro Gln Glu Val Thr 
    50                  55                  60                  


Tyr Thr Gln Leu Asn His Cys Val Phe Thr Gln Arg Lys Ile Thr Arg 
65                  70                  75                  80  


Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Ile Ile Val Tyr Thr 
                85                  90                  95      


Glu Leu Pro Asn Ala Glu Ser Arg Ser Lys Val Val Ser Cys Pro 
            100                 105                 110     


<210>  28
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR2DL4 endodomain

<400>  28

Gly Ile Ala Arg His Leu His Ala Val Ile Arg Tyr Ser Val Ala Ile 
1               5                   10                  15      


Ile Leu Phe Thr Ile Leu Pro Phe Phe Leu Leu His Arg Trp Cys Ser 
            20                  25                  30          


Lys Lys Lys Glu Asn Ala Ala Val Met Asn Gln Glu Pro Ala Gly His 
        35                  40                  45              


Arg Thr Val Asn Arg Glu Asp Ser Asp Glu Gln Asp Pro Gln Glu Val 
    50                  55                  60                  


Thr Tyr Ala Gln Leu Asp His Cys Ile Phe Thr Gln Arg Lys Ile Thr 
65                  70                  75                  80  


Gly Pro Ser Gln Arg Ser Lys Arg Pro Ser Thr Asp Thr Ser Val Cys 
                85                  90                  95      


Ile Glu Leu Pro Asn Ala Glu Pro Arg Ala Leu Ser Pro Ala His Glu 
            100                 105                 110         


His His Ser Gln Ala Leu Met Gly Ser Ser Arg Glu Thr Thr Ala Leu 
        115                 120                 125             


Ser Gln Thr Gln Leu Ala Ser Ser Asn Val Pro Ala Ala Gly Ile 
    130                 135                 140             


<210>  29
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR2DL5 endodomain

<400>  29

Thr Gly Ile Arg Arg His Leu His Ile Leu Ile Gly Thr Ser Val Ala 
1               5                   10                  15      


Ile Ile Leu Phe Ile Ile Leu Phe Phe Phe Leu Leu His Cys Cys Cys 
            20                  25                  30          


Ser Asn Lys Lys Asn Ala Ala Val Met Asp Gln Glu Pro Ala Gly Asp 
        35                  40                  45              


Arg Thr Val Asn Arg Glu Asp Ser Asp Asp Gln Asp Pro Gln Glu Val 
    50                  55                  60                  


Thr Tyr Ala Gln Leu Asp His Cys Val Phe Thr Gln Thr Lys Ile Thr 
65                  70                  75                  80  


Ser Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Thr Thr Met Tyr 
                85                  90                  95      


Met Glu Leu Pro Asn Ala Lys Pro Arg Ser Leu Ser Pro Ala His Lys 
            100                 105                 110         


His His Ser Gln Ala Leu Arg Gly Ser Ser Arg Glu Thr Thr Ala Leu 
        115                 120                 125             


Ser Gln Asn Arg Val Ala Ser Ser His Val Pro Ala Ala Gly Ile 
    130                 135                 140             


<210>  30
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR3DL1 endodomain

<400>  30

Lys Asp Pro Arg His Leu His Ile Leu Ile Gly Thr Ser Val Val Ile 
1               5                   10                  15      


Ile Leu Phe Ile Leu Leu Leu Phe Phe Leu Leu His Leu Trp Cys Ser 
            20                  25                  30          


Asn Lys Lys Asn Ala Ala Val Met Asp Gln Glu Pro Ala Gly Asn Arg 
        35                  40                  45              


Thr Ala Asn Ser Glu Asp Ser Asp Glu Gln Asp Pro Glu Glu Val Thr 
    50                  55                  60                  


Tyr Ala Gln Leu Asp His Cys Val Phe Thr Gln Arg Lys Ile Thr Arg 
65                  70                  75                  80  


Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Thr Ile Leu Tyr Thr 
                85                  90                  95      


Glu Leu Pro Asn Ala Lys Pro Arg Ser Lys Val Val Ser Cys Pro 
            100                 105                 110     


<210>  31
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR3DL3 endodomain

<400>  31

Lys Asp Pro Gly Asn Ser Arg His Leu His Val Leu Ile Gly Thr Ser 
1               5                   10                  15      


Val Val Ile Ile Pro Phe Ala Ile Leu Leu Phe Phe Leu Leu His Arg 
            20                  25                  30          


Trp Cys Ala Asn Lys Lys Asn Ala Val Val Met Asp Gln Glu Pro Ala 
        35                  40                  45              


Gly Asn Arg Thr Val Asn Arg Glu Asp Ser Asp Glu Gln Asp Pro Gln 
    50                  55                  60                  


Glu Val Thr Tyr Ala Gln Leu Asn His Cys Val Phe Thr Gln Arg Lys 
65                  70                  75                  80  


Ile Thr Arg Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Thr Ser 
                85                  90                  95      


Val 
    


<210>  32
<211>  807
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6-CD45 fusion protein

<400>  32

Trp Tyr His Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln 
1               5                   10                  15      


Ala Lys Gly Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln 
            20                  25                  30          


Pro Gly Asp Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly 
        35                  40                  45              


Pro Gly Ser Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly 
    50                  55                  60                  


Gly Arg Tyr Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp 
65                  70                  75                  80  


Leu Val Glu His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala 
                85                  90                  95      


Phe Val Tyr Leu Arg Gln Pro Tyr Lys Ile Tyr Asp Leu His Lys Lys 
            100                 105                 110         


Arg Ser Cys Asn Leu Asp Glu Gln Gln Glu Leu Val Glu Arg Asp Asp 
        115                 120                 125             


Glu Lys Gln Leu Met Asn Val Glu Pro Ile His Ala Asp Ile Leu Leu 
    130                 135                 140                 


Glu Thr Tyr Lys Arg Lys Ile Ala Asp Glu Gly Arg Leu Phe Leu Ala 
145                 150                 155                 160 


Glu Phe Gln Ser Ile Pro Arg Val Phe Ser Lys Phe Pro Ile Lys Glu 
                165                 170                 175     


Ala Arg Lys Pro Phe Asn Gln Asn Lys Asn Arg Tyr Val Asp Ile Leu 
            180                 185                 190         


Pro Tyr Asp Tyr Asn Arg Val Glu Leu Ser Glu Ile Asn Gly Asp Ala 
        195                 200                 205             


Gly Ser Asn Tyr Ile Asn Ala Ser Tyr Ile Asp Gly Phe Lys Glu Pro 
    210                 215                 220                 


Arg Lys Tyr Ile Ala Ala Gln Gly Pro Arg Asp Glu Thr Val Asp Asp 
225                 230                 235                 240 


Phe Trp Arg Met Ile Trp Glu Gln Lys Ala Thr Val Ile Val Met Val 
                245                 250                 255     


Thr Arg Cys Glu Glu Gly Asn Arg Asn Lys Cys Ala Glu Tyr Trp Pro 
            260                 265                 270         


Ser Met Glu Glu Gly Thr Arg Ala Phe Gly Asp Val Val Val Lys Ile 
        275                 280                 285             


Asn Gln His Lys Arg Cys Pro Asp Tyr Ile Ile Gln Lys Leu Asn Ile 
    290                 295                 300                 


Val Asn Lys Lys Glu Lys Ala Thr Gly Arg Glu Val Thr His Ile Gln 
305                 310                 315                 320 


Phe Thr Ser Trp Pro Asp His Gly Val Pro Glu Asp Pro His Leu Leu 
                325                 330                 335     


Leu Lys Leu Arg Arg Arg Val Asn Ala Phe Ser Asn Phe Phe Ser Gly 
            340                 345                 350         


Pro Ile Val Val His Cys Ser Ala Gly Val Gly Arg Thr Gly Thr Tyr 
        355                 360                 365             


Ile Gly Ile Asp Ala Met Leu Glu Gly Leu Glu Ala Glu Asn Lys Val 
    370                 375                 380                 


Asp Val Tyr Gly Tyr Val Val Lys Leu Arg Arg Gln Arg Cys Leu Met 
385                 390                 395                 400 


Val Gln Val Glu Ala Gln Tyr Ile Leu Ile His Gln Ala Leu Val Glu 
                405                 410                 415     


Tyr Asn Gln Phe Gly Glu Thr Glu Val Asn Leu Ser Glu Leu His Pro 
            420                 425                 430         


Tyr Leu His Asn Met Lys Lys Arg Asp Pro Pro Ser Glu Pro Ser Pro 
        435                 440                 445             


Leu Glu Ala Glu Phe Gln Arg Leu Pro Ser Tyr Arg Ser Trp Arg Thr 
    450                 455                 460                 


Gln His Ile Gly Asn Gln Glu Glu Asn Lys Ser Lys Asn Arg Asn Ser 
465                 470                 475                 480 


Asn Val Ile Pro Tyr Asp Tyr Asn Arg Val Leu Lys His Glu Leu Glu 
                485                 490                 495     


Met Ser Lys Glu Ser Glu His Asp Ser Asp Glu Ser Ser Asp Asp Asp 
            500                 505                 510         


Ser Asp Ser Glu Glu Pro Ser Lys Tyr Ile Asn Ala Ser Phe Ile Met 
        515                 520                 525             


Ser Tyr Trp Lys Pro Glu Val Met Ile Ala Ala Gln Gly Pro Leu Lys 
    530                 535                 540                 


Glu Thr Ile Gly Asp Phe Met Ile Gln Arg Lys Val Lys Val Ile Val 
545                 550                 555                 560 


Met Leu Thr Glu Leu Lys His Gly Asp Gln Glu Ile Cys Ala Gln Tyr 
                565                 570                 575     


Trp Gly Glu Gly Lys Gln Thr Tyr Gly Asp Ile Glu Val Asp Leu Lys 
            580                 585                 590         


Asp Thr Asp Lys Ser Ser Thr Tyr Thr Leu Arg Val Phe Glu Leu Arg 
        595                 600                 605             


His Ser Lys Arg Lys Asp Ser Arg Thr Val Tyr Gln Tyr Gln Tyr Thr 
    610                 615                 620                 


Asn Trp Ser Val Glu Gln Leu Pro Ala Glu Pro Lys Glu Leu Ile Ser 
625                 630                 635                 640 


Met Ile Gln Val Val Lys Gln Lys Leu Pro Gln Lys Asn Ser Ser Glu 
                645                 650                 655     


Gly Asn Lys His His Lys Ser Thr Pro Leu Leu Ile His Cys Arg Asp 
            660                 665                 670         


Gly Ser Gln Gln Thr Gly Ile Phe Cys Ala Leu Leu Asn Leu Leu Glu 
        675                 680                 685             


Ser Ala Glu Thr Glu Glu Val Val Asp Ile Phe Gln Val Val Lys Ala 
    690                 695                 700                 


Leu Arg Lys Ala Arg Pro Gly Met Val Ser Thr Phe Glu Gln Tyr Gln 
705                 710                 715                 720 


Phe Leu Tyr Asp Val Ile Ala Ser Thr Tyr Pro Ala Gln Asn Gly Gln 
                725                 730                 735     


Val Lys Lys Asn Asn His Gln Glu Asp Lys Ile Glu Phe Asp Asn Glu 
            740                 745                 750         


Val Asp Lys Val Lys Gln Asp Ala Asn Cys Val Asn Pro Leu Gly Ala 
        755                 760                 765             


Pro Glu Lys Leu Pro Glu Ala Lys Glu Gln Ala Glu Gly Ser Glu Pro 
    770                 775                 780                 


Thr Ser Gly Thr Glu Gly Pro Glu His Ser Val Asn Gly Pro Ala Ser 
785                 790                 795                 800 


Pro Ala Leu Asn Gln Gly Ser 
                805         


<210>  33
<211>  434
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6-CD148 fusion protein

<400>  33

Glu Thr Leu Leu Gln Ala Lys Gly Glu Pro Trp Thr Phe Leu Val Arg 
1               5                   10                  15      


Glu Ser Leu Ser Gln Pro Gly Asp Phe Val Leu Ser Val Leu Ser Asp 
            20                  25                  30          


Gln Pro Lys Ala Gly Pro Gly Ser Pro Leu Arg Val Thr His Ile Lys 
        35                  40                  45              


Val Met Cys Glu Gly Gly Arg Tyr Thr Val Gly Gly Leu Glu Thr Phe 
    50                  55                  60                  


Asp Ser Leu Thr Asp Leu Val Glu His Phe Lys Lys Thr Gly Ile Glu 
65                  70                  75                  80  


Glu Ala Ser Gly Ala Phe Val Tyr Leu Arg Gln Pro Tyr Arg Lys Lys 
                85                  90                  95      


Arg Lys Asp Ala Lys Asn Asn Glu Val Ser Phe Ser Gln Ile Lys Pro 
            100                 105                 110         


Lys Lys Ser Lys Leu Ile Arg Val Glu Asn Phe Glu Ala Tyr Phe Lys 
        115                 120                 125             


Lys Gln Gln Ala Asp Ser Asn Cys Gly Phe Ala Glu Glu Tyr Glu Asp 
    130                 135                 140                 


Leu Lys Leu Val Gly Ile Ser Gln Pro Lys Tyr Ala Ala Glu Leu Ala 
145                 150                 155                 160 


Glu Asn Arg Gly Lys Asn Arg Tyr Asn Asn Val Leu Pro Tyr Asp Ile 
                165                 170                 175     


Ser Arg Val Lys Leu Ser Val Gln Thr His Ser Thr Asp Asp Tyr Ile 
            180                 185                 190         


Asn Ala Asn Tyr Met Pro Gly Tyr His Ser Lys Lys Asp Phe Ile Ala 
        195                 200                 205             


Thr Gln Gly Pro Leu Pro Asn Thr Leu Lys Asp Phe Trp Arg Met Val 
    210                 215                 220                 


Trp Glu Lys Asn Val Tyr Ala Ile Ile Met Leu Thr Lys Cys Val Glu 
225                 230                 235                 240 


Gln Gly Arg Thr Lys Cys Glu Glu Tyr Trp Pro Ser Lys Gln Ala Gln 
                245                 250                 255     


Asp Tyr Gly Asp Ile Thr Val Ala Met Thr Ser Glu Ile Val Leu Pro 
            260                 265                 270         


Glu Trp Thr Ile Arg Asp Phe Thr Val Lys Asn Ile Gln Thr Ser Glu 
        275                 280                 285             


Ser His Pro Leu Arg Gln Phe His Phe Thr Ser Trp Pro Asp His Gly 
    290                 295                 300                 


Val Pro Asp Thr Thr Asp Leu Leu Ile Asn Phe Arg Tyr Leu Val Arg 
305                 310                 315                 320 


Asp Tyr Met Lys Gln Ser Pro Pro Glu Ser Pro Ile Leu Val His Cys 
                325                 330                 335     


Ser Ala Gly Val Gly Arg Thr Gly Thr Phe Ile Ala Ile Asp Arg Leu 
            340                 345                 350         


Ile Tyr Gln Ile Glu Asn Glu Asn Thr Val Asp Val Tyr Gly Ile Val 
        355                 360                 365             


Tyr Asp Leu Arg Met His Arg Pro Leu Met Val Gln Thr Glu Asp Gln 
    370                 375                 380                 


Tyr Val Phe Leu Asn Gln Cys Val Leu Asp Ile Val Arg Ser Gln Lys 
385                 390                 395                 400 


Asp Ser Lys Val Asp Leu Ile Tyr Gln Asn Thr Thr Ala Met Thr Ile 
                405                 410                 415     


Tyr Glu Asn Leu Ala Pro Val Thr Thr Phe Gly Lys Thr Asn Gly Tyr 
            420                 425                 430         


Ile Ala 
        


<210>  34
<211>  20
<212>  PRT
<213>  Foot-and-mouth disease virus

<400>  34

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  35
<211>  3390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequences coding for CARs 
       (MP13974.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-HCH2CH3pvaa-CD
       28tmZw)

<400>  35
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggcc     1440

gtgcccactc aggtcctggg gttgttgcta ctgtggctta cagatgccag atgtgacatc     1500

cagatgacac agtctccatc ttccctgtct gcatctgtcg gagatcgcgt caccatcacc     1560

tgtcgagcaa gtgaggacat ttattttaat ttagtgtggt atcagcagaa accaggaaag     1620

gcccctaagc tcctgatcta tgatacaaat cgcttggcag atggggtccc atcacggttc     1680

agtggctctg gatctggcac acagtatact ctaaccataa gtagcctgca acccgaagat     1740

ttcgcaacct attattgtca acactataag aattatccgc tcacgttcgg tcaggggacc     1800

aagctggaaa tcaaaagatc tggtggcgga gggtcaggag gcggaggcag cggaggcggt     1860

ggctcgggag gcggaggctc gagatctgag gtgcagttgg tggagtctgg gggcggcttg     1920

gtgcagcctg gagggtccct gaggctctcc tgtgcagcct caggattcac tctcagtaat     1980

tatggcatgc actggatcag gcaggctcca gggaagggtc tggagtgggt ctcgtctatt     2040

agtcttaatg gtggtagcac ttactatcga gactccgtga agggccgatt cactatctcc     2100

agggacaatg caaaaagcac cctctacctt caaatgaata gtctgagggc cgaggacacg     2160

gccgtctatt actgtgcagc acaggacgct tatacgggag gttactttga ttactggggc     2220

caaggaacgc tggtcacagt ctcgtctatg gatcccgccg agcccaaatc tcctgacaaa     2280

actcacacat gcccaccgtg cccagcacct cccgtggccg gcccgtcagt cttcctcttc     2340

cccccaaaac ccaaggacac cctcatgatc gcccggaccc ctgaggtcac atgcgtggtg     2400

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag     2460

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc     2520

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc     2580

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc     2640

cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc     2700

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     2760

aatgggcaac cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     2820

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     2880

tcatgctccg tgatgcatga ggccctgcac aatcactata cccagaaatc tctgagtctg     2940

agcccaggca agaaggaccc caagttctgg gtcctggtgg tggtgggagg cgtgctggcc     3000

tgttactctc tcctggtgac cgtggccttc atcatctttt gggtgcgctc ccgggtgaag     3060

ttttctcgct ctgccgatgc cccagcctat cagcagggcc agaatcagct gtacaatgaa     3120

ctgaacctgg gcaggcggga ggagtacgac gtgctggata agcggagagg cagagacccc     3180

gagatgggcg gcaaaccacg gcgcaaaaat ccccaggagg gactctataa cgagctgcag     3240

aaggacaaaa tggccgaggc ctattccgag atcggcatga agggagagag aagacgcgga     3300

aagggccacg acggcctgta tcagggattg tccaccgcta caaaagatac atatgatgcc     3360

ctgcacatgc aggccctgcc acccagatga                                      3390


<210>  36
<211>  5154
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequences coding for CARs 
       (MP14802.SFG.aCD19fmc63_clean-CD8STK-CD28tmZ-2A-aCD33glx-HCH2CH3p
       vaa-dCD45)

<400>  36
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggcc     1440

gtgcccactc aggtcctggg gttgttgcta ctgtggctta cagatgccag atgtgacatc     1500

cagatgacac agtctccatc ttccctgtct gcatctgtcg gagatcgcgt caccatcacc     1560

tgtcgagcaa gtgaggacat ttattttaat ttagtgtggt atcagcagaa accaggaaag     1620

gcccctaagc tcctgatcta tgatacaaat cgcttggcag atggggtccc atcacggttc     1680

agtggctctg gatctggcac acagtatact ctaaccataa gtagcctgca acccgaagat     1740

ttcgcaacct attattgtca acactataag aattatccgc tcacgttcgg tcaggggacc     1800

aagctggaaa tcaaaagatc tggtggcgga gggtcaggag gcggaggcag cggaggcggt     1860

ggctcgggag gcggaggctc gagatctgag gtgcagttgg tggagtctgg gggcggcttg     1920

gtgcagcctg gagggtccct gaggctctcc tgtgcagcct caggattcac tctcagtaat     1980

tatggcatgc actggatcag gcaggctcca gggaagggtc tggagtgggt ctcgtctatt     2040

agtcttaatg gtggtagcac ttactatcga gactccgtga agggccgatt cactatctcc     2100

agggacaatg caaaaagcac cctctacctt caaatgaata gtctgagggc cgaggacacg     2160

gccgtctatt actgtgcagc acaggacgct tatacgggag gttactttga ttactggggc     2220

caaggaacgc tggtcacagt ctcgtctatg gatcccgccg agcccaaatc tcctgacaaa     2280

actcacacat gcccaccgtg cccagcacct cccgtggccg gcccgtcagt cttcctcttc     2340

cccccaaaac ccaaggacac cctcatgatc gcccggaccc ctgaggtcac atgcgtggtg     2400

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag     2460

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc     2520

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc     2580

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc     2640

cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc     2700

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     2760

aatgggcaac cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     2820

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     2880

tcatgctccg tgatgcatga ggccctgcac aatcactata cccagaaatc tctgagtctg     2940

agcccaggca agaaggaccc caaggcactg atagcatttc tggcatttct gattattgtg     3000

acatcaatag ccctgcttgt tgttctctac aaaatctatg atctacataa gaaaagatcc     3060

tgcaatttag atgaacagca ggagcttgtt gaaagggatg atgaaaaaca actgatgaat     3120

gtggagccaa tccatgcaga tattttgttg gaaacttata agaggaagat tgctgatgaa     3180

ggaagacttt ttctggctga atttcagagc atcccgcggg tgttcagcaa gtttcctata     3240

aaggaagctc gaaagccctt taaccagaat aaaaaccgtt atgttgacat tcttccttat     3300

gattataacc gtgttgaact ctctgagata aacggagatg cagggtcaaa ctacataaat     3360

gccagctata ttgatggttt caaagaaccc aggaaataca ttgctgcaca aggtcccagg     3420

gatgaaactg ttgatgattt ctggaggatg atttgggaac agaaagccac agttattgtc     3480

atggtcactc gatgtgaaga aggaaacagg aacaagtgtg cagaatactg gccgtcaatg     3540

gaagagggca ctcgggcttt tggagatgtt gttgtaaaga tcaaccagca caaaagatgt     3600

ccagattaca tcattcagaa attgaacatt gtaaataaaa aagaaaaagc aactggaaga     3660

gaggtgactc acattcagtt caccagctgg ccagaccacg gggtgcctga ggatcctcac     3720

ttgctcctca aactgagaag gagagtgaat gccttcagca atttcttcag tggtcccatt     3780

gtggtgcact gcagtgctgg tgttgggcgc acaggaacct atatcggaat tgatgccatg     3840

ctagaaggcc tggaagccga gaacaaagtg gatgtttatg gttatgttgt caagctaagg     3900

cgacagagat gcctgatggt tcaagtagag gcccagtaca tcttgatcca tcaggctttg     3960

gtggaataca atcagtttgg agaaacagaa gtgaatttgt ctgaattaca tccatatcta     4020

cataacatga agaaaaggga tccacccagt gagccgtctc cactagaggc tgaattccag     4080

agacttcctt catataggag ctggaggaca cagcacattg gaaatcaaga agaaaataaa     4140

agtaaaaaca ggaattctaa tgtcatccca tatgactata acagagtgcc acttaaacat     4200

gagctggaaa tgagtaaaga gagtgagcat gattcagatg aatcctctga tgatgacagt     4260

gattcagagg aaccaagcaa atacatcaat gcatctttta taatgagcta ctggaaacct     4320

gaagtgatga ttgctgctca gggaccactg aaggagacca ttggtgactt ttggcagatg     4380

atcttccaaa gaaaagtcaa agttattgtt atgctgacag aactgaaaca tggagaccag     4440

gaaatctgtg ctcagtactg gggagaagga aagcaaacat atggagatat tgaagttgac     4500

ctgaaagaca cagacaaatc ttcaacttat acccttcgtg tctttgaact gagacattcc     4560

aagaggaaag actctcgaac tgtgtaccag taccaatata caaactggag tgtggagcag     4620

cttcctgcag aacccaagga attaatctct atgattcagg tcgtcaaaca aaaacttccc     4680

cagaagaatt cctctgaagg gaacaagcat cacaagagta cacctctact cattcactgc     4740

agggatggat ctcagcaaac gggaatattt tgtgctttgt taaatctctt agaaagtgcg     4800

gaaacagaag aggtagtgga tatttttcaa gtggtaaaag ctctacgcaa agctaggcca     4860

ggcatggttt ccacattcga gcaatatcaa ttcctatatg acgtcattgc cagcacctac     4920

cctgctcaga atggacaagt aaagaaaaac aaccatcaag aagataaaat tgaatttgat     4980

aatgaagtgg acaaagtaaa gcaggatgct aattgtgtta atccacttgg tgccccagaa     5040

aagctccctg aagcaaagga acaggctgaa ggttctgaac ccacgagtgg cactgagggg     5100

ccagaacatt ctgtcaatgg tcctgcaagt ccagctttaa atcaaggttc atag           5154


<210>  37
<211>  4053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequences coding for CARs 
       (MP14801.SFG.aCD19fmc63_clean-CD8STK-CD28tmZ-2A-aCD33glx-HCH2CH3p
       vaa-dCD148)

<400>  37
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggcc     1440

gtgcccactc aggtcctggg gttgttgcta ctgtggctta cagatgccag atgtgacatc     1500

cagatgacac agtctccatc ttccctgtct gcatctgtcg gagatcgcgt caccatcacc     1560

tgtcgagcaa gtgaggacat ttattttaat ttagtgtggt atcagcagaa accaggaaag     1620

gcccctaagc tcctgatcta tgatacaaat cgcttggcag atggggtccc atcacggttc     1680

agtggctctg gatctggcac acagtatact ctaaccataa gtagcctgca acccgaagat     1740

ttcgcaacct attattgtca acactataag aattatccgc tcacgttcgg tcaggggacc     1800

aagctggaaa tcaaaagatc tggtggcgga gggtcaggag gcggaggcag cggaggcggt     1860

ggctcgggag gcggaggctc gagatctgag gtgcagttgg tggagtctgg gggcggcttg     1920

gtgcagcctg gagggtccct gaggctctcc tgtgcagcct caggattcac tctcagtaat     1980

tatggcatgc actggatcag gcaggctcca gggaagggtc tggagtgggt ctcgtctatt     2040

agtcttaatg gtggtagcac ttactatcga gactccgtga agggccgatt cactatctcc     2100

agggacaatg caaaaagcac cctctacctt caaatgaata gtctgagggc cgaggacacg     2160

gccgtctatt actgtgcagc acaggacgct tatacgggag gttactttga ttactggggc     2220

caaggaacgc tggtcacagt ctcgtctatg gatcccgccg agcccaaatc tcctgacaaa     2280

actcacacat gcccaccgtg cccagcacct cccgtggccg gcccgtcagt cttcctcttc     2340

cccccaaaac ccaaggacac cctcatgatc gcccggaccc ctgaggtcac atgcgtggtg     2400

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag     2460

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc     2520

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc     2580

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc     2640

cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc     2700

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     2760

aatgggcaac cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     2820

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     2880

tcatgctccg tgatgcatga ggccctgcac aatcactata cccagaaatc tctgagtctg     2940

agcccaggca agaaggaccc caaggcggtt tttggctgta tctttggtgc cctggttatt     3000

gtgactgtgg gaggcttcat cttctggaga aagaagagga aagatgcaaa gaataatgaa     3060

gtgtcctttt ctcaaattaa acctaaaaaa tctaagttaa tcagagtgga gaattttgag     3120

gcctacttca agaagcagca agctgactcc aactgtgggt tcgcagagga atacgaagat     3180

ctgaagcttg ttggaattag tcaacctaaa tatgcagcag aactggctga gaatagagga     3240

aagaatcgct ataataatgt tctgccctat gatatttccc gtgtcaaact ttcggtccag     3300

acccattcaa cggatgacta catcaatgcc aactacatgc ctggctacca ctccaagaaa     3360

gattttattg ccacacaagg acctttaccg aacactttga aagatttttg gcgtatggtt     3420

tgggagaaaa atgtatatgc catcattatg ttgactaaat gtgttgaaca gggaagaacc     3480

aaatgtgagg agtattggcc ctccaagcag gctcaggact atggagacat aactgtggca     3540

atgacatcag aaattgttct tccggaatgg accatcagag atttcacagt gaaaaatatc     3600

cagacaagtg agagtcaccc tctgagacag ttccatttca cctcctggcc agaccacggt     3660

gttcccgaca ccactgacct gctcatcaac ttccggtacc tcgttcgtga ctacatgaag     3720

cagagtcctc ccgaatcgcc gattctggtg cattgcagtg ctggggtcgg aaggacgggc     3780

actttcattg ccattgatcg tctcatctac cagatagaga atgagaacac cgtggatgtg     3840

tatgggattg tgtatgacct tcgaatgcat aggcctttaa tggtgcagac agaggaccag     3900

tatgttttcc tcaatcagtg tgttttggat attgtcagat cccagaaaga ctcaaaagta     3960

gatcttatct accagaacac aactgcaatg acaatctatg aaaaccttgc gcccgtgacc     4020

acatttggaa agaccaatgg ttacatcgcc taa                                  4053


<210>  38
<211>  3345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequences coding for CARs 
       (16076.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-muCD8STK-tm-dPTP
       N6)

<400>  38
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggcc     1440

gtgcccactc aggtcctggg gttgttgcta ctgtggctta cagatgccag atgtgacatc     1500

cagatgacac agtctccatc ttccctgtct gcatctgtcg gagatcgcgt caccatcacc     1560

tgtcgagcaa gtgaggacat ttattttaat ttagtgtggt atcagcagaa accaggaaag     1620

gcccctaagc tcctgatcta tgatacaaat cgcttggcag atggggtccc atcacggttc     1680

agtggctctg gatctggcac acagtatact ctaaccataa gtagcctgca acccgaagat     1740

ttcgcaacct attattgtca acactataag aattatccgc tcacgttcgg tcaggggacc     1800

aagctggaaa tcaaaagatc tggtggcgga gggtcaggag gcggaggcag cggaggcggt     1860

ggctcgggag gcggaggctc gagatctgag gtgcagttgg tggagtctgg gggcggcttg     1920

gtgcagcctg gagggtccct gaggctctcc tgtgcagcct caggattcac tctcagtaat     1980

tatggcatgc actggatcag gcaggctcca gggaagggtc tggagtgggt ctcgtctatt     2040

agtcttaatg gtggtagcac ttactatcga gactccgtga agggccgatt cactatctcc     2100

agggacaatg caaaaagcac cctctacctt caaatgaata gtctgagggc cgaggacacg     2160

gccgtctatt actgtgcagc acaggacgct tatacgggag gttactttga ttactggggc     2220

caaggaacgc tggtcacagt ctcgtctatg gatcccgcca ccacaaccaa gcccgtgctg     2280

cggaccccaa gccctgtgca ccctaccggc accagccagc ctcagagacc cgaggactgc     2340

cggcctcggg gcagcgtgaa gggcaccggc ctggacttcg cctgcgacat ctactgggca     2400

cctctggccg gaatatgcgt ggcactgctg ctgagcctca tcatcaccct gatctgttat     2460

caccgaagcc gcaagcgggt gtgtaaaagt ggaggcggaa gcttctggga ggagtttgag     2520

agtttgcaga agcaggaggt gaagaacttg caccagcgtc tggaagggca gcggccagag     2580

aacaagggca agaaccgcta caagaacatt ctcccctttg accacagccg agtgatcctg     2640

cagggacggg acagtaacat ccccgggtcc gactacatca atgccaacta catcaagaac     2700

cagctgctag gccctgatga gaacgctaag acctacatcg ccagccaggg ctgtctggag     2760

gccacggtca atgacttctg gcagatggcg tggcaggaga acagccgtgt catcgtcatg     2820

accacccgag aggtggagaa aggccggaac aaatgcgtcc catactggcc cgaggtgggc     2880

atgcagcgtg cttatgggcc ctactctgtg accaactgcg gggagcatga cacaaccgaa     2940

tacaaactcc gtaccttaca ggtctccccg ctggacaatg gagacctgat tcgggagatc     3000

tggcattacc agtacctgag ctggcccgac cacggggtcc ccagtgagcc tgggggtgtc     3060

ctcagcttcc tggaccagat caaccagcgg caggaaagtc tgcctcacgc agggcccatc     3120

atcgtgcact gcagcgccgg catcggccgc acaggcacca tcattgtcat cgacatgctc     3180

atggagaaca tctccaccaa gggcctggac tgtgacattg acatccagaa gaccatccag     3240

atggtgcggg cgcagcgctc gggcatggtg cagacggagg cgcagtacaa gttcatctac     3300

gtggccatcg cccagttcat tgaaaccact aagaagaagc tgtga                     3345


<210>  39
<211>  2757
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequences coding for CARs 
       (MP16091.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-muCD8STK-LAIR1
       tm-endo)

<400>  39
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggcc     1440

gtgcccactc aggtcctggg gttgttgcta ctgtggctta cagatgccag atgtgacatc     1500

cagatgacac agtctccatc ttccctgtct gcatctgtcg gagatcgcgt caccatcacc     1560

tgtcgagcaa gtgaggacat ttattttaat ttagtgtggt atcagcagaa accaggaaag     1620

gcccctaagc tcctgatcta tgatacaaat cgcttggcag atggggtccc atcacggttc     1680

agtggctctg gatctggcac acagtatact ctaaccataa gtagcctgca acccgaagat     1740

ttcgcaacct attattgtca acactataag aattatccgc tcacgttcgg tcaggggacc     1800

aagctggaaa tcaaaagatc tggtggcgga gggtcaggag gcggaggcag cggaggcggt     1860

ggctcgggag gcggaggctc gagatctgag gtgcagttgg tggagtctgg gggcggcttg     1920

gtgcagcctg gagggtccct gaggctctcc tgtgcagcct caggattcac tctcagtaat     1980

tatggcatgc actggatcag gcaggctcca gggaagggtc tggagtgggt ctcgtctatt     2040

agtcttaatg gtggtagcac ttactatcga gactccgtga agggccgatt cactatctcc     2100

agggacaatg caaaaagcac cctctacctt caaatgaata gtctgagggc cgaggacacg     2160

gccgtctatt actgtgcagc acaggacgct tatacgggag gttactttga ttactggggc     2220

caaggaacgc tggtcacagt ctcgtctatg gatcccgcca ccacaaccaa gcccgtgctg     2280

cggaccccaa gccctgtgca ccctaccggc accagccagc ctcagagacc cgaggactgc     2340

cggcctcggg gcagcgtgaa gggcaccggc ctggacttcg cctgcgacat tctcatcggg     2400

gtctcagtgg tcttcctctt ctgtctcctc ctcctggtcc tcttctgcct ccatcgccag     2460

aatcagataa agcaggggcc ccccagaagc aaggacgagg agcagaagcc acagcagagg     2520

cctgacctgg ctgttgatgt tctagagagg acagcagaca aggccacagt caatggactt     2580

cctgagaagg accgggagac cgacaccagc gccctggctg cagggagttc ccaggaggtg     2640

acgtatgctc agctggacca ctgggccctc acacagagga cagcccgggc tgtgtcccca     2700

cagtccacaa agcccatggc cgagtccatc acgtatgcag ccgttgccag acactga        2757


<210>  40
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequences coding for CARs 
       (MP16092.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-muCD8STK-LAIR1
       tm-endo-2A-PTPN6_SH2-dCD148)

<400>  40
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggcc     1440

gtgcccactc aggtcctggg gttgttgcta ctgtggctta cagatgccag atgtgacatc     1500

cagatgacac agtctccatc ttccctgtct gcatctgtcg gagatcgcgt caccatcacc     1560

tgtcgagcaa gtgaggacat ttattttaat ttagtgtggt atcagcagaa accaggaaag     1620

gcccctaagc tcctgatcta tgatacaaat cgcttggcag atggggtccc atcacggttc     1680

agtggctctg gatctggcac acagtatact ctaaccataa gtagcctgca acccgaagat     1740

ttcgcaacct attattgtca acactataag aattatccgc tcacgttcgg tcaggggacc     1800

aagctggaaa tcaaaagatc tggtggcgga gggtcaggag gcggaggcag cggaggcggt     1860

ggctcgggag gcggaggctc gagatctgag gtgcagttgg tggagtctgg gggcggcttg     1920

gtgcagcctg gagggtccct gaggctctcc tgtgcagcct caggattcac tctcagtaat     1980

tatggcatgc actggatcag gcaggctcca gggaagggtc tggagtgggt ctcgtctatt     2040

agtcttaatg gtggtagcac ttactatcga gactccgtga agggccgatt cactatctcc     2100

agggacaatg caaaaagcac cctctacctt caaatgaata gtctgagggc cgaggacacg     2160

gccgtctatt actgtgcagc acaggacgct tatacgggag gttactttga ttactggggc     2220

caaggaacgc tggtcacagt ctcgtctatg gatcccgcca ccacaaccaa gcccgtgctg     2280

cggaccccaa gccctgtgca ccctaccggc accagccagc ctcagagacc cgaggactgc     2340

cggcctcggg gcagcgtgaa gggcaccggc ctggacttcg cctgcgacat tctcatcggg     2400

gtctcagtgg tcttcctctt ctgtctcctc ctcctggtcc tcttctgcct ccatcgccag     2460

aatcagataa agcaggggcc ccccagaagc aaggacgagg agcagaagcc acagcagagg     2520

cctgacctgg ctgttgatgt tctagagagg acagcagaca aggccacagt caatggactt     2580

cctgagaagg accgggagac cgacaccagc gccctggctg cagggagttc ccaggaggtg     2640

acgtatgctc agctggacca ctgggccctc acacagagga cagcccgggc tgtgtcccca     2700

cagtccacaa agcccatggc cgagtccatc acgtatgcag ccgttgccag acacagggca     2760

gaaggaagag gtagcctgct gacttgcggg gacgtggaag agaacccagg gccatggtat     2820

catggccaca tgtctggcgg gcaggcagag acgctgctgc aggccaaggg cgagccctgg     2880

acgtttcttg tgcgtgagag cctcagccag cctggagact tcgtgctttc tgtgctcagt     2940

gaccagccca aggctggccc aggctccccg ctcagggtca cccacatcaa ggtcatgtgc     3000

gagggtggac gctacacagt gggtggtttg gagaccttcg acagcctcac ggacctggtg     3060

gagcatttca agaagacggg gattgaggag gcctcaggcg cctttgtcta cctgcggcag     3120

ccgtacagcg gtggcggtgg cagctttgag gcctacttca agaagcagca agctgactcc     3180

aactgtgggt tcgcagagga atacgaagat ctgaagcttg ttggaattag tcaacctaaa     3240

tatgcagcag aactggctga gaatagagga aagaatcgct ataataatgt tctgccctat     3300

gatatttccc gtgtcaaact ttcggtccag acccattcaa cggatgacta catcaatgcc     3360

aactacatgc ctggctacca ctccaagaaa gattttattg ccacacaagg acctttaccg     3420

aacactttga aagatttttg gcgtatggtt tgggagaaaa atgtatatgc catcattatg     3480

ttgactaaat gtgttgaaca gggaagaacc aaatgtgagg agtattggcc ctccaagcag     3540

gctcaggact atggagacat aactgtggca atgacatcag aaattgttct tccggaatgg     3600

accatcagag atttcacagt gaaaaatatc cagacaagtg agagtcaccc tctgagacag     3660

ttccatttca cctcctggcc agaccacggt gttcccgaca ccactgacct gctcatcaac     3720

ttccggtacc tcgttcgtga ctacatgaag cagagtcctc ccgaatcgcc gattctggtg     3780

cattgcagtg ctggggtcgg aaggacgggc actttcattg ccattgatcg tctcatctac     3840

cagatagaga atgagaacac cgtggatgtg tatgggattg tgtatgacct tcgaatgcat     3900

aggcctttaa tggtgcagac agaggaccag tatgttttcc tcaatcagtg tgttttggat     3960

attgtcagat cccagaaaga ctcaaaagta gatcttatct accagaacac aactgcaatg     4020

acaatctatg aaaaccttgc gcccgtgacc acatttggaa agaccaatgg ttacatcgcc     4080

agcggtagct aa                                                         4092


<210>  41
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single-chain variable fragment (scFv)  
       SFG.aCD19-CD8STK-CD28tmZ-2A-aGD2-HCH2CH3pvaa-dCD148

<400>  41

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Glu 
465                 470                 475                 480 


Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro Gly Ser 
                485                 490                 495     


Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro 
            500                 505                 510         


Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Ala 
        515                 520                 525             


Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 
    530                 535                 540                 


Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala 
545                 550                 555                 560 


Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn Gln Val 
                565                 570                 575     


Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val Tyr Tyr 
            580                 585                 590         


Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp Gly Gln 
        595                 600                 605             


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    610                 615                 620                 


Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser Pro Ser 
625                 630                 635                 640 


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys Arg Ala 
                645                 650                 655     


Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln Lys Ser 
            660                 665                 670         


Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu Ala Ser 
        675                 680                 685             


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr 
    690                 695                 700                 


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
705                 710                 715                 720 


Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr Lys Val 
                725                 730                 735     


Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr 
            740                 745                 750         


His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val 
        755                 760                 765             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr 
    770                 775                 780                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
785                 790                 795                 800 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                805                 810                 815     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            820                 825                 830         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        835                 840                 845             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    850                 855                 860                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
865                 870                 875                 880 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                885                 890                 895     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            900                 905                 910         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        915                 920                 925             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    930                 935                 940                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
945                 950                 955                 960 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys 
                965                 970                 975     


Asp Pro Lys Ala Val Phe Gly Cys Ile Phe Gly Ala Leu Val Ile Val 
            980                 985                 990         


Thr Val Gly Gly Phe Ile Phe Trp  Arg Lys Lys Arg Lys  Asp Ala Lys 
        995                 1000                 1005             


Asn Asn  Glu Val Ser Phe Ser  Gln Ile Lys Pro Lys  Lys Ser Lys 
    1010                 1015                 1020             


Leu Ile  Arg Val Glu Asn Phe  Glu Ala Tyr Phe Lys  Lys Gln Gln 
    1025                 1030                 1035             


Ala Asp  Ser Asn Cys Gly Phe  Ala Glu Glu Tyr Glu  Asp Leu Lys 
    1040                 1045                 1050             


Leu Val  Gly Ile Ser Gln Pro  Lys Tyr Ala Ala Glu  Leu Ala Glu 
    1055                 1060                 1065             


Asn Arg  Gly Lys Asn Arg Tyr  Asn Asn Val Leu Pro  Tyr Asp Ile 
    1070                 1075                 1080             


Ser Arg  Val Lys Leu Ser Val  Gln Thr His Ser Thr  Asp Asp Tyr 
    1085                 1090                 1095             


Ile Asn  Ala Asn Tyr Met Pro  Gly Tyr His Ser Lys  Lys Asp Phe 
    1100                 1105                 1110             


Ile Ala  Thr Gln Gly Pro Leu  Pro Asn Thr Leu Lys  Asp Phe Trp 
    1115                 1120                 1125             


Arg Met  Val Trp Glu Lys Asn  Val Tyr Ala Ile Ile  Met Leu Thr 
    1130                 1135                 1140             


Lys Cys  Val Glu Gln Gly Arg  Thr Lys Cys Glu Glu  Tyr Trp Pro 
    1145                 1150                 1155             


Ser Lys  Gln Ala Gln Asp Tyr  Gly Asp Ile Thr Val  Ala Met Thr 
    1160                 1165                 1170             


Ser Glu  Ile Val Leu Pro Glu  Trp Thr Ile Arg Asp  Phe Thr Val 
    1175                 1180                 1185             


Lys Asn  Ile Gln Thr Ser Glu  Ser His Pro Leu Arg  Gln Phe His 
    1190                 1195                 1200             


Phe Thr  Ser Trp Pro Asp His  Gly Val Pro Asp Thr  Thr Asp Leu 
    1205                 1210                 1215             


Leu Ile  Asn Phe Arg Tyr Leu  Val Arg Asp Tyr Met  Lys Gln Ser 
    1220                 1225                 1230             


Pro Pro  Glu Ser Pro Ile Leu  Val His Cys Ser Ala  Gly Val Gly 
    1235                 1240                 1245             


Arg Thr  Gly Thr Phe Ile Ala  Ile Asp Arg Leu Ile  Tyr Gln Ile 
    1250                 1255                 1260             


Glu Asn  Glu Asn Thr Val Asp  Val Tyr Gly Ile Val  Tyr Asp Leu 
    1265                 1270                 1275             


Arg Met  His Arg Pro Leu Met  Val Gln Thr Glu Asp  Gln Tyr Val 
    1280                 1285                 1290             


Phe Leu  Asn Gln Cys Val Leu  Asp Ile Val Arg Ser  Gln Lys Asp 
    1295                 1300                 1305             


Ser Lys  Val Asp Leu Ile Tyr  Gln Asn Thr Thr Ala  Met Thr Ile 
    1310                 1315                 1320             


Tyr Glu  Asn Leu Ala Pro Val  Thr Thr Phe Gly Lys  Thr Asn Gly 
    1325                 1330                 1335             


Tyr Ile  Ala 
    1340     


<210>  42
<211>  4026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single-chain variable fragment (scFv) 
       SFG.aCD19-CD8STK-CD28tmZ-2A-aGD2-HCH2CH3pvaa-dCD148

<400>  42
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggag     1440

accgacaccc tgctgctgtg ggtgctgctg ctgtgggtgc caggcagcac cggccaggtg     1500

cagctgcagg agtctggccc aggcctggtg aagcccagcc agaccctgag catcacctgc     1560

accgtgagcg gcttcagcct ggccagctac aacatccact gggtgcggca gcccccaggc     1620

aagggcctgg agtggctggg cgtgatctgg gctggcggca gcaccaacta caacagcgcc     1680

ctgatgagcc ggctgaccat cagcaaggac aacagcaaga accaggtgtt cctgaagatg     1740

agcagcctga cagccgccga caccgccgtg tactactgcg ccaagcggag cgacgactac     1800

agctggttcg cctactgggg ccagggcacc ctggtgaccg tgagctctgg cggaggcggc     1860

tctggcggag gcggctctgg cggaggcggc agcgagaacc agatgaccca gagccccagc     1920

agcttgagcg ccagcgtggg cgaccgggtg accatgacct gcagagccag cagcagcgtg     1980

agcagcagct acctgcactg gtaccagcag aagagcggca aggccccaaa ggtgtggatc     2040

tacagcacca gcaacctggc cagcggcgtg cccagccggt tcagcggcag cggcagcggc     2100

accgactaca ccctgaccat cagcagcctg cagcccgagg acttcgccac ctactactgc     2160

cagcagtaca gcggctaccc catcaccttc ggccagggca ccaaggtgga gatcaagcgg     2220

tcggatcccg ccgagcccaa atctcctgac aaaactcaca catgcccacc gtgcccagca     2280

cctcccgtgg ccggcccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg     2340

atcgcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     2400

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     2460

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     2520

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc     2580

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc     2640

ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt caaaggcttc     2700

tatcccagcg acatcgccgt ggagtgggag agcaatgggc aaccggagaa caactacaag     2760

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg     2820

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggccctg     2880

cacaatcact atacccagaa atctctgagt ctgagcccag gcaagaagga ccccaaggcg     2940

gtttttggct gtatctttgg tgccctggtt attgtgactg tgggaggctt catcttctgg     3000

agaaagaaga ggaaagatgc aaagaataat gaagtgtcct tttctcaaat taaacctaaa     3060

aaatctaagt taatcagagt ggagaatttt gaggcctact tcaagaagca gcaagctgac     3120

tccaactgtg ggttcgcaga ggaatacgaa gatctgaagc ttgttggaat tagtcaacct     3180

aaatatgcag cagaactggc tgagaataga ggaaagaatc gctataataa tgttctgccc     3240

tatgatattt cccgtgtcaa actttcggtc cagacccatt caacggatga ctacatcaat     3300

gccaactaca tgcctggcta ccactccaag aaagatttta ttgccacaca aggaccttta     3360

ccgaacactt tgaaagattt ttggcgtatg gtttgggaga aaaatgtata tgccatcatt     3420

atgttgacta aatgtgttga acagggaaga accaaatgtg aggagtattg gccctccaag     3480

caggctcagg actatggaga cataactgtg gcaatgacat cagaaattgt tcttccggaa     3540

tggaccatca gagatttcac agtgaaaaat atccagacaa gtgagagtca ccctctgaga     3600

cagttccatt tcacctcctg gccagaccac ggtgttcccg acaccactga cctgctcatc     3660

aacttccggt acctcgttcg tgactacatg aagcagagtc ctcccgaatc gccgattctg     3720

gtgcattgca gtgctggggt cggaaggacg ggcactttca ttgccattga tcgtctcatc     3780

taccagatag agaatgagaa caccgtggat gtgtatggga ttgtgtatga ccttcgaatg     3840

cataggcctt taatggtgca gacagaggac cagtatgttt tcctcaatca gtgtgttttg     3900

gatattgtca gatcccagaa agactcaaaa gtagatctta tctaccagaa cacaactgca     3960

atgacaatct atgaaaacct tgcgcccgtg accacatttg gaaagaccaa tggttacatc     4020

gcctaa                                                                4026


<210>  43
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single-chain variable fragment (scFv) 
       SFG.aCD19-CD8STK-CD28tmZ-2A-aCD5-HCH2CH3pvaa-dCD148

<400>  43

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Glu 
465                 470                 475                 480 


Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro Gly Ser 
                485                 490                 495     


Thr Gly Gln Val Thr Leu Lys Glu Ser Gly Pro Gly Ile Leu Lys Pro 
            500                 505                 510         


Ser Gln Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu Ser 
        515                 520                 525             


Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Ser Gly Lys Gly 
    530                 535                 540                 


Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Val Tyr Tyr Asn 
545                 550                 555                 560 


Pro Ser Leu Lys Asn Gln Leu Thr Ile Ser Lys Asp Ala Ser Arg Asp 
                565                 570                 575     


Gln Val Phe Leu Lys Ile Thr Asn Leu Asp Thr Ala Asp Thr Ala Thr 
            580                 585                 590         


Tyr Tyr Cys Val Arg Arg Arg Ala Thr Gly Thr Gly Phe Asp Tyr Trp 
        595                 600                 605             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    610                 615                 620                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn Ile Val Met Thr Gln Ser 
625                 630                 635                 640 


His Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val Ser Ile Ala Cys 
                645                 650                 655     


Lys Ala Ser Gln Asp Val Gly Thr Ala Val Ala Trp Tyr Gln Gln Lys 
            660                 665                 670         


Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Thr Ser Thr Arg His 
        675                 680                 685             


Thr Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
    690                 695                 700                 


Thr Leu Thr Ile Thr Asn Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe 
705                 710                 715                 720 


Cys His Gln Tyr Asn Ser Tyr Asn Thr Phe Gly Ser Gly Thr Arg Leu 
                725                 730                 735     


Glu Leu Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr 
            740                 745                 750         


His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val 
        755                 760                 765             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr 
    770                 775                 780                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
785                 790                 795                 800 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                805                 810                 815     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            820                 825                 830         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        835                 840                 845             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    850                 855                 860                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
865                 870                 875                 880 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                885                 890                 895     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            900                 905                 910         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        915                 920                 925             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    930                 935                 940                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
945                 950                 955                 960 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys 
                965                 970                 975     


Asp Pro Lys Ala Val Phe Gly Cys Ile Phe Gly Ala Leu Val Ile Val 
            980                 985                 990         


Thr Val Gly Gly Phe Ile Phe Trp  Arg Lys Lys Arg Lys  Asp Ala Lys 
        995                 1000                 1005             


Asn Asn  Glu Val Ser Phe Ser  Gln Ile Lys Pro Lys  Lys Ser Lys 
    1010                 1015                 1020             


Leu Ile  Arg Val Glu Asn Phe  Glu Ala Tyr Phe Lys  Lys Gln Gln 
    1025                 1030                 1035             


Ala Asp  Ser Asn Cys Gly Phe  Ala Glu Glu Tyr Glu  Asp Leu Lys 
    1040                 1045                 1050             


Leu Val  Gly Ile Ser Gln Pro  Lys Tyr Ala Ala Glu  Leu Ala Glu 
    1055                 1060                 1065             


Asn Arg  Gly Lys Asn Arg Tyr  Asn Asn Val Leu Pro  Tyr Asp Ile 
    1070                 1075                 1080             


Ser Arg  Val Lys Leu Ser Val  Gln Thr His Ser Thr  Asp Asp Tyr 
    1085                 1090                 1095             


Ile Asn  Ala Asn Tyr Met Pro  Gly Tyr His Ser Lys  Lys Asp Phe 
    1100                 1105                 1110             


Ile Ala  Thr Gln Gly Pro Leu  Pro Asn Thr Leu Lys  Asp Phe Trp 
    1115                 1120                 1125             


Arg Met  Val Trp Glu Lys Asn  Val Tyr Ala Ile Ile  Met Leu Thr 
    1130                 1135                 1140             


Lys Cys  Val Glu Gln Gly Arg  Thr Lys Cys Glu Glu  Tyr Trp Pro 
    1145                 1150                 1155             


Ser Lys  Gln Ala Gln Asp Tyr  Gly Asp Ile Thr Val  Ala Met Thr 
    1160                 1165                 1170             


Ser Glu  Ile Val Leu Pro Glu  Trp Thr Ile Arg Asp  Phe Thr Val 
    1175                 1180                 1185             


Lys Asn  Ile Gln Thr Ser Glu  Ser His Pro Leu Arg  Gln Phe His 
    1190                 1195                 1200             


Phe Thr  Ser Trp Pro Asp His  Gly Val Pro Asp Thr  Thr Asp Leu 
    1205                 1210                 1215             


Leu Ile  Asn Phe Arg Tyr Leu  Val Arg Asp Tyr Met  Lys Gln Ser 
    1220                 1225                 1230             


Pro Pro  Glu Ser Pro Ile Leu  Val His Cys Ser Ala  Gly Val Gly 
    1235                 1240                 1245             


Arg Thr  Gly Thr Phe Ile Ala  Ile Asp Arg Leu Ile  Tyr Gln Ile 
    1250                 1255                 1260             


Glu Asn  Glu Asn Thr Val Asp  Val Tyr Gly Ile Val  Tyr Asp Leu 
    1265                 1270                 1275             


Arg Met  His Arg Pro Leu Met  Val Gln Thr Glu Asp  Gln Tyr Val 
    1280                 1285                 1290             


Phe Leu  Asn Gln Cys Val Leu  Asp Ile Val Arg Ser  Gln Lys Asp 
    1295                 1300                 1305             


Ser Lys  Val Asp Leu Ile Tyr  Gln Asn Thr Thr Ala  Met Thr Ile 
    1310                 1315                 1320             


Tyr Glu  Asn Leu Ala Pro Val  Thr Thr Phe Gly Lys  Thr Asn Gly 
    1325                 1330                 1335             


Tyr Ile  Ala 
    1340     


<210>  44
<211>  4026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single-chain variable fragment (scFv) 
       SFG.aCD19-CD8STK-CD28tmZ-2A-aCD5-HCH2CH3pvaa-dCD148

<400>  44
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggag     1440

accgacaccc tgctgctgtg ggtgctgctg ctgtgggtgc ccggcagcac cggccaggtg     1500

accctgaagg agagcggtcc cggcatcctg aagcccagcc agaccctgag cctgacctgc     1560

agcttcagcg gcttcagcct gagcaccagc ggcatgggcg tgggctggat tcggcagccc     1620

agcggcaagg gcctggagtg gctggcccac atctggtggg acgacgacgt gtactacaac     1680

cccagcctga agaaccagct gaccatcagc aaggacgcca gccgggacca ggtgttcctg     1740

aagatcacca acctggacac cgccgacacc gccacctact actgcgtgcg gcgccgggcc     1800

accggcaccg gcttcgacta ctggggccag ggcaccaccc tgaccgtgag cagcggtggc     1860

ggtggcagcg gcggcggcgg aagcggaggt ggtggcagca acatcgtgat gacccagagc     1920

cacaagttca tgagcaccag cgtgggcgac cgggtgagca tcgcctgcaa ggccagccag     1980

gacgtgggca ccgccgtggc ctggtaccag cagaagcctg gccagagccc caagctgctg     2040

atctactgga ccagcacccg gcacaccggc gtgcccgacc ggttcaccgg cagcggcagc     2100

ggcaccgact tcaccctgac catcaccaac gtgcagagcg aggacctggc cgactacttc     2160

tgccaccagt acaacagcta caacaccttc ggcagcggca cccggctgga gctgaagcgg     2220

tcggatcccg ccgagcccaa atctcctgac aaaactcaca catgcccacc gtgcccagca     2280

cctcccgtgg ccggcccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg     2340

atcgcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     2400

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     2460

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     2520

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc     2580

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc     2640

ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt caaaggcttc     2700

tatcccagcg acatcgccgt ggagtgggag agcaatgggc aaccggagaa caactacaag     2760

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg     2820

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggccctg     2880

cacaatcact atacccagaa atctctgagt ctgagcccag gcaagaagga ccccaaggcg     2940

gtttttggct gtatctttgg tgccctggtt attgtgactg tgggaggctt catcttctgg     3000

agaaagaaga ggaaagatgc aaagaataat gaagtgtcct tttctcaaat taaacctaaa     3060

aaatctaagt taatcagagt ggagaatttt gaggcctact tcaagaagca gcaagctgac     3120

tccaactgtg ggttcgcaga ggaatacgaa gatctgaagc ttgttggaat tagtcaacct     3180

aaatatgcag cagaactggc tgagaataga ggaaagaatc gctataataa tgttctgccc     3240

tatgatattt cccgtgtcaa actttcggtc cagacccatt caacggatga ctacatcaat     3300

gccaactaca tgcctggcta ccactccaag aaagatttta ttgccacaca aggaccttta     3360

ccgaacactt tgaaagattt ttggcgtatg gtttgggaga aaaatgtata tgccatcatt     3420

atgttgacta aatgtgttga acagggaaga accaaatgtg aggagtattg gccctccaag     3480

caggctcagg actatggaga cataactgtg gcaatgacat cagaaattgt tcttccggaa     3540

tggaccatca gagatttcac agtgaaaaat atccagacaa gtgagagtca ccctctgaga     3600

cagttccatt tcacctcctg gccagaccac ggtgttcccg acaccactga cctgctcatc     3660

aacttccggt acctcgttcg tgactacatg aagcagagtc ctcccgaatc gccgattctg     3720

gtgcattgca gtgctggggt cggaaggacg ggcactttca ttgccattga tcgtctcatc     3780

taccagatag agaatgagaa caccgtggat gtgtatggga ttgtgtatga ccttcgaatg     3840

cataggcctt taatggtgca gacagaggac cagtatgttt tcctcaatca gtgtgttttg     3900

gatattgtca gatcccagaa agactcaaaa gtagatctta tctaccagaa cacaactgca     3960

atgacaatct atgaaaacct tgcgcccgtg accacatttg gaaagaccaa tggttacatc     4020

gcctaa                                                                4026


<210>  45
<211>  1342
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single-chain variable fragment (scFv) 
       SFG.aCD19-CD8STK-CD28tmZ-2A-aEGFRvIII-HCH2CH3pvaa-dCD148

<400>  45

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Glu 
465                 470                 475                 480 


Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro Gly Ser 
                485                 490                 495     


Thr Gly Gln Val Lys Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro 
            500                 505                 510         


Gly Ala Ser Leu Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Arg 
        515                 520                 525             


Lys Phe Gly Met Ser Trp Val Arg Gln Thr Ser Asp Lys Arg Leu Glu 
    530                 535                 540                 


Trp Val Ala Ser Ile Ser Thr Gly Gly Tyr Asn Thr Tyr Tyr Ser Asp 
545                 550                 555                 560 


Asn Val Lys Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Lys Asn Thr 
                565                 570                 575     


Leu Tyr Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Leu Tyr 
            580                 585                 590         


Tyr Cys Thr Arg Gly Tyr Ser Ser Thr Ser Tyr Ala Met Asp Tyr Trp 
        595                 600                 605             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    610                 615                 620                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Glu Leu Thr Gln Ser 
625                 630                 635                 640 


Pro Ala Ser Leu Ser Val Ala Thr Gly Glu Lys Val Thr Ile Arg Cys 
                645                 650                 655     


Met Thr Ser Thr Asp Ile Asp Asp Asp Met Asn Trp Tyr Gln Gln Lys 
            660                 665                 670         


Pro Gly Glu Pro Pro Lys Phe Leu Ile Ser Glu Gly Asn Thr Leu Arg 
        675                 680                 685             


Pro Gly Val Pro Ser Arg Phe Ser Ser Ser Gly Thr Gly Thr Asp Phe 
    690                 695                 700                 


Val Phe Thr Ile Glu Asn Thr Leu Ser Glu Asp Val Gly Asp Tyr Tyr 
705                 710                 715                 720 


Cys Leu Gln Ser Phe Asn Val Pro Leu Thr Phe Gly Asp Gly Thr Lys 
                725                 730                 735     


Leu Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys 
            740                 745                 750         


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser 
        755                 760                 765             


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg 
    770                 775                 780                 


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
785                 790                 795                 800 


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
                805                 810                 815     


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
            820                 825                 830         


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
        835                 840                 845             


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
    850                 855                 860                 


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
865                 870                 875                 880 


Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 
                885                 890                 895     


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
            900                 905                 910         


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
        915                 920                 925             


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
    930                 935                 940                 


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
945                 950                 955                 960 


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                965                 970                 975     


Lys Asp Pro Lys Ala Val Phe Gly Cys Ile Phe Gly Ala Leu Val Ile 
            980                 985                 990         


Val Thr Val Gly Gly Phe Ile Phe  Trp Arg Lys Lys Arg  Lys Asp Ala 
        995                 1000                 1005             


Lys Asn  Asn Glu Val Ser Phe  Ser Gln Ile Lys Pro  Lys Lys Ser 
    1010                 1015                 1020             


Lys Leu  Ile Arg Val Glu Asn  Phe Glu Ala Tyr Phe  Lys Lys Gln 
    1025                 1030                 1035             


Gln Ala  Asp Ser Asn Cys Gly  Phe Ala Glu Glu Tyr  Glu Asp Leu 
    1040                 1045                 1050             


Lys Leu  Val Gly Ile Ser Gln  Pro Lys Tyr Ala Ala  Glu Leu Ala 
    1055                 1060                 1065             


Glu Asn  Arg Gly Lys Asn Arg  Tyr Asn Asn Val Leu  Pro Tyr Asp 
    1070                 1075                 1080             


Ile Ser  Arg Val Lys Leu Ser  Val Gln Thr His Ser  Thr Asp Asp 
    1085                 1090                 1095             


Tyr Ile  Asn Ala Asn Tyr Met  Pro Gly Tyr His Ser  Lys Lys Asp 
    1100                 1105                 1110             


Phe Ile  Ala Thr Gln Gly Pro  Leu Pro Asn Thr Leu  Lys Asp Phe 
    1115                 1120                 1125             


Trp Arg  Met Val Trp Glu Lys  Asn Val Tyr Ala Ile  Ile Met Leu 
    1130                 1135                 1140             


Thr Lys  Cys Val Glu Gln Gly  Arg Thr Lys Cys Glu  Glu Tyr Trp 
    1145                 1150                 1155             


Pro Ser  Lys Gln Ala Gln Asp  Tyr Gly Asp Ile Thr  Val Ala Met 
    1160                 1165                 1170             


Thr Ser  Glu Ile Val Leu Pro  Glu Trp Thr Ile Arg  Asp Phe Thr 
    1175                 1180                 1185             


Val Lys  Asn Ile Gln Thr Ser  Glu Ser His Pro Leu  Arg Gln Phe 
    1190                 1195                 1200             


His Phe  Thr Ser Trp Pro Asp  His Gly Val Pro Asp  Thr Thr Asp 
    1205                 1210                 1215             


Leu Leu  Ile Asn Phe Arg Tyr  Leu Val Arg Asp Tyr  Met Lys Gln 
    1220                 1225                 1230             


Ser Pro  Pro Glu Ser Pro Ile  Leu Val His Cys Ser  Ala Gly Val 
    1235                 1240                 1245             


Gly Arg  Thr Gly Thr Phe Ile  Ala Ile Asp Arg Leu  Ile Tyr Gln 
    1250                 1255                 1260             


Ile Glu  Asn Glu Asn Thr Val  Asp Val Tyr Gly Ile  Val Tyr Asp 
    1265                 1270                 1275             


Leu Arg  Met His Arg Pro Leu  Met Val Gln Thr Glu  Asp Gln Tyr 
    1280                 1285                 1290             


Val Phe  Leu Asn Gln Cys Val  Leu Asp Ile Val Arg  Ser Gln Lys 
    1295                 1300                 1305             


Asp Ser  Lys Val Asp Leu Ile  Tyr Gln Asn Thr Thr  Ala Met Thr 
    1310                 1315                 1320             


Ile Tyr  Glu Asn Leu Ala Pro  Val Thr Thr Phe Gly  Lys Thr Asn 
    1325                 1330                 1335             


Gly Tyr  Ile Ala 
    1340         


<210>  46
<211>  4029
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single-chain variable fragment (scFv) 
       SFG.aCD19-CD8STK-CD28tmZ-2A-aEGFRvIII-HCH2CH3pvaa-dCD148

<400>  46
atgagcctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgccaga       60

ccagacatcc agatgaccca gaccaccagc agcctgagcg ccagcctggg cgaccgggtg      120

accatcagct gcagagccag ccaggacatc agcaagtacc tgaactggta ccagcagaag      180

cccgacggca ccgtgaagct gctgatctac cacaccagcc ggctgcacag cggcgtgccc      240

agccggttca gcggcagcgg cagcggcacc gactacagcc tgaccatcag caacctggag      300

caggaggaca tcgccaccta cttctgccag cagggcaaca ccctgcccta caccttcgga      360

ggcggcacca agctggagat caccaaggcc ggaggcggag gctctggcgg aggcggctct      420

ggcggaggcg gctctggcgg aggcggcagc gaggtgaagc tgcaggagtc tggcccaggc      480

ctggtggccc caagccagag cctgagcgtg acctgcaccg tgagcggcgt gagcctgccc      540

gactacggcg tgagctggat caggcagccc ccacggaagg gcctggagtg gctgggcgtg      600

atctggggca gcgagaccac ctactacaac agcgccctga agagccggct gaccatcatc      660

aaggacaaca gcaagagcca ggtgttcctg aagatgaaca gcctgcagac cgacgacacc      720

gccatctact actgcgccaa gcactactac tatggcggca gctacgctat ggactactgg      780

ggccagggca ccagcgtgac cgtgagctca gatcccacca cgacgccagc gccgcgacca      840

ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg      900

ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga tatcttttgg      960

gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt     1020

attattttct gggtgaggag agtgaagttc agcaggagcg cagacgcccc cgcgtaccag     1080

cagggccaga accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt     1140

ttggacaaga gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct     1200

caggaaggcc tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt     1260

gggatgaaag gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt     1320

acagccacca aggacaccta cgacgccctt cacatgcagg ccctgcctcc tcgcagagcc     1380

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg gcccatggag     1440

accgacaccc tgctgctgtg ggtgctgctg ctgtgggtgc ccggcagcac cggccaggtg     1500

aagctgcagc agagcggcgg aggcctggtg aagcccggcg ccagcctgaa gctgagctgc     1560

gtgaccagcg gcttcacctt ccggaagttc ggcatgagct gggtgcggca gaccagcgac     1620

aagcggctgg agtgggtggc cagcatcagc accggcggct acaacaccta ctacagcgac     1680

aacgtgaagg gccggttcac catcagccgg gagaacgcca agaacaccct gtacctgcag     1740

atgagcagcc tgaagagcga ggacaccgcc ctgtactact gcacccgggg ctacagcagc     1800

accagctacg ctatggacta ctggggccag ggcaccaccg tgacagtgag cagcggcgga     1860

ggaggcagtg gtgggggtgg atctggcgga ggtggcagcg acatcgagct gacccagagc     1920

cccgccagcc tgagcgtggc caccggcgag aaggtgacca tccggtgcat gaccagcacc     1980

gacatcgacg acgacatgaa ctggtaccag cagaagcccg gcgagccccc aaagttcctg     2040

atcagcgagg gcaacaccct gcggcccggc gtgcccagcc ggttcagcag cagcggcacc     2100

ggcaccgact tcgtgttcac catcgagaac accctgagcg aggacgtggg cgactactac     2160

tgcctgcaga gcttcaacgt gcccctgacc ttcggcgacg gcaccaagct ggagatcaag     2220

cggtcggatc ccgccgagcc caaatctcct gacaaaactc acacatgccc accgtgccca     2280

gcacctcccg tggccggccc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc     2340

atgatcgccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct     2400

gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg     2460

cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag     2520

gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc     2580

atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg     2640

cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc     2700

ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcaaccgga gaacaactac     2760

aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc     2820

gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggcc     2880

ctgcacaatc actataccca gaaatctctg agtctgagcc caggcaagaa ggaccccaag     2940

gcggtttttg gctgtatctt tggtgccctg gttattgtga ctgtgggagg cttcatcttc     3000

tggagaaaga agaggaaaga tgcaaagaat aatgaagtgt ccttttctca aattaaacct     3060

aaaaaatcta agttaatcag agtggagaat tttgaggcct acttcaagaa gcagcaagct     3120

gactccaact gtgggttcgc agaggaatac gaagatctga agcttgttgg aattagtcaa     3180

cctaaatatg cagcagaact ggctgagaat agaggaaaga atcgctataa taatgttctg     3240

ccctatgata tttcccgtgt caaactttcg gtccagaccc attcaacgga tgactacatc     3300

aatgccaact acatgcctgg ctaccactcc aagaaagatt ttattgccac acaaggacct     3360

ttaccgaaca ctttgaaaga tttttggcgt atggtttggg agaaaaatgt atatgccatc     3420

attatgttga ctaaatgtgt tgaacaggga agaaccaaat gtgaggagta ttggccctcc     3480

aagcaggctc aggactatgg agacataact gtggcaatga catcagaaat tgttcttccg     3540

gaatggacca tcagagattt cacagtgaaa aatatccaga caagtgagag tcaccctctg     3600

agacagttcc atttcacctc ctggccagac cacggtgttc ccgacaccac tgacctgctc     3660

atcaacttcc ggtacctcgt tcgtgactac atgaagcaga gtcctcccga atcgccgatt     3720

ctggtgcatt gcagtgctgg ggtcggaagg acgggcactt tcattgccat tgatcgtctc     3780

atctaccaga tagagaatga gaacaccgtg gatgtgtatg ggattgtgta tgaccttcga     3840

atgcataggc ctttaatggt gcagacagag gaccagtatg ttttcctcaa tcagtgtgtt     3900

ttggatattg tcagatccca gaaagactca aaagtagatc ttatctacca gaacacaact     3960

gcaatgacaa tctatgaaaa ccttgcgccc gtgaccacat ttggaaagac caatggttac     4020

atcgcctaa                                                             4029


<210>  47
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Immunoreceptor tyrosine-based inhibition motif (ITIM)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Ser, Ile, Val or Leu

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa may be Ile, Val or Leu

<400>  47

Xaa Xaa Tyr Xaa Xaa Xaa 
1               5       


<210>  48
<211>  1114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a AND NOT gate 
       (16076.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-muCD8STK-tm-dPTP
       N6)

<400>  48

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro 
        755                 760                 765             


Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly 
    770                 775                 780                 


Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Tyr Trp Ala 
785                 790                 795                 800 


Pro Leu Ala Gly Ile Cys Val Ala Leu Leu Leu Ser Leu Ile Ile Thr 
                805                 810                 815     


Leu Ile Cys Tyr His Arg Ser Arg Lys Arg Val Cys Lys Ser Gly Gly 
            820                 825                 830         


Gly Ser Phe Trp Glu Glu Phe Glu Ser Leu Gln Lys Gln Glu Val Lys 
        835                 840                 845             


Asn Leu His Gln Arg Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly Lys 
    850                 855                 860                 


Asn Arg Tyr Lys Asn Ile Leu Pro Phe Asp His Ser Arg Val Ile Leu 
865                 870                 875                 880 


Gln Gly Arg Asp Ser Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala Asn 
                885                 890                 895     


Tyr Ile Lys Asn Gln Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr Tyr 
            900                 905                 910         


Ile Ala Ser Gln Gly Cys Leu Glu Ala Thr Val Asn Asp Phe Trp Gln 
        915                 920                 925             


Met Ala Trp Gln Glu Asn Ser Arg Val Ile Val Met Thr Thr Arg Glu 
    930                 935                 940                 


Val Glu Lys Gly Arg Asn Lys Cys Val Pro Tyr Trp Pro Glu Val Gly 
945                 950                 955                 960 


Met Gln Arg Ala Tyr Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu His 
                965                 970                 975     


Asp Thr Thr Glu Tyr Lys Leu Arg Thr Leu Gln Val Ser Pro Leu Asp 
            980                 985                 990         


Asn Gly Asp Leu Ile Arg Glu Ile  Trp His Tyr Gln Tyr  Leu Ser Trp 
        995                 1000                 1005             


Pro Asp  His Gly Val Pro Ser  Glu Pro Gly Gly Val  Leu Ser Phe 
    1010                 1015                 1020             


Leu Asp  Gln Ile Asn Gln Arg  Gln Glu Ser Leu Pro  His Ala Gly 
    1025                 1030                 1035             


Pro Ile  Ile Val His Cys Ser  Ala Gly Ile Gly Arg  Thr Gly Thr 
    1040                 1045                 1050             


Ile Ile  Val Ile Asp Met Leu  Met Glu Asn Ile Ser  Thr Lys Gly 
    1055                 1060                 1065             


Leu Asp  Cys Asp Ile Asp Ile  Gln Lys Thr Ile Gln  Met Val Arg 
    1070                 1075                 1080             


Ala Gln  Arg Ser Gly Met Val  Gln Thr Glu Ala Gln  Tyr Lys Phe 
    1085                 1090                 1095             


Ile Tyr  Val Ala Ile Ala Gln  Phe Ile Glu Thr Thr  Lys Lys Lys 
    1100                 1105                 1110             


Leu 
    


<210>  49
<211>  918
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a AND NOT gate 
       (MP16091.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-muCD8STK-LAIR1
       tm-endo)

<400>  49

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Ala Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro 
        755                 760                 765             


Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly 
    770                 775                 780                 


Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Leu Ile Gly 
785                 790                 795                 800 


Val Ser Val Val Phe Leu Phe Cys Leu Leu Leu Leu Val Leu Phe Cys 
                805                 810                 815     


Leu His Arg Gln Asn Gln Ile Lys Gln Gly Pro Pro Arg Ser Lys Asp 
            820                 825                 830         


Glu Glu Gln Lys Pro Gln Gln Arg Pro Asp Leu Ala Val Asp Val Leu 
        835                 840                 845             


Glu Arg Thr Ala Asp Lys Ala Thr Val Asn Gly Leu Pro Glu Lys Asp 
    850                 855                 860                 


Arg Glu Thr Asp Thr Ser Ala Leu Ala Ala Gly Ser Ser Gln Glu Val 
865                 870                 875                 880 


Thr Tyr Ala Gln Leu Asp His Trp Ala Leu Thr Gln Arg Thr Ala Arg 
                885                 890                 895     


Ala Val Ser Pro Gln Ser Thr Lys Pro Met Ala Glu Ser Ile Thr Tyr 
            900                 905                 910         


Ala Ala Val Ala Arg His 
        915             


<210>  50
<211>  1362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a AND NOT gate 
       (MP16092.SFG.aCD19fmc63-CD8STK-CD28tmZ-2A-aCD33glx-muCD8STK-LAIR1
       tm-endo-2A-PTPN6_SH2-dCD148)

<400>  50

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
    450                 455                 460                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
465                 470                 475                 480 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
                485                 490                 495     


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            500                 505                 510         


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
        515                 520                 525             


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    530                 535                 540                 


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
545                 550                 555                 560 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
                565                 570                 575     


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
            580                 585                 590         


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    610                 615                 620                 


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
625                 630                 635                 640 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                645                 650                 655     


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
            660                 665                 670         


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
        675                 680                 685             


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    690                 695                 700                 


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
705                 710                 715                 720 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
                725                 730                 735     


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
            740                 745                 750         


Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro Thr 
        755                 760                 765             


Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser 
    770                 775                 780                 


Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Leu Ile Gly Val 
785                 790                 795                 800 


Ser Val Val Phe Leu Phe Cys Leu Leu Leu Leu Val Leu Phe Cys Leu 
                805                 810                 815     


His Arg Gln Asn Gln Ile Lys Gln Gly Pro Pro Arg Ser Lys Asp Glu 
            820                 825                 830         


Glu Gln Lys Pro Gln Gln Arg Pro Asp Leu Ala Val Asp Val Leu Glu 
        835                 840                 845             


Arg Thr Ala Asp Lys Ala Thr Val Asn Gly Leu Pro Glu Lys Asp Arg 
    850                 855                 860                 


Glu Thr Asp Thr Ser Ala Leu Ala Ala Gly Ser Ser Gln Glu Val Thr 
865                 870                 875                 880 


Tyr Ala Gln Leu Asp His Trp Ala Leu Thr Gln Arg Thr Ala Arg Ala 
                885                 890                 895     


Val Ser Pro Gln Ser Thr Lys Pro Met Ala Glu Ser Ile Thr Tyr Ala 
            900                 905                 910         


Ala Val Ala Arg His Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys 
        915                 920                 925             


Gly Asp Val Glu Glu Asn Pro Gly Pro Trp Tyr His Gly His Met Ser 
    930                 935                 940                 


Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly Glu Pro Trp Thr 
945                 950                 955                 960 


Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp Phe Val Leu Ser 
                965                 970                 975     


Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser Pro Leu Arg Val 
            980                 985                 990         


Thr His Ile Lys Val Met Cys Glu  Gly Gly Arg Tyr Thr  Val Gly Gly 
        995                 1000                 1005             


Leu Glu  Thr Phe Asp Ser Leu  Thr Asp Leu Val Glu  His Phe Lys 
    1010                 1015                 1020             


Lys Thr  Gly Ile Glu Glu Ala  Ser Gly Ala Phe Val  Tyr Leu Arg 
    1025                 1030                 1035             


Gln Pro  Tyr Ser Gly Gly Gly  Gly Ser Phe Glu Ala  Tyr Phe Lys 
    1040                 1045                 1050             


Lys Gln  Gln Ala Asp Ser Asn  Cys Gly Phe Ala Glu  Glu Tyr Glu 
    1055                 1060                 1065             


Asp Leu  Lys Leu Val Gly Ile  Ser Gln Pro Lys Tyr  Ala Ala Glu 
    1070                 1075                 1080             


Leu Ala  Glu Asn Arg Gly Lys  Asn Arg Tyr Asn Asn  Val Leu Pro 
    1085                 1090                 1095             


Tyr Asp  Ile Ser Arg Val Lys  Leu Ser Val Gln Thr  His Ser Thr 
    1100                 1105                 1110             


Asp Asp  Tyr Ile Asn Ala Asn  Tyr Met Pro Gly Tyr  His Ser Lys 
    1115                 1120                 1125             


Lys Asp  Phe Ile Ala Thr Gln  Gly Pro Leu Pro Asn  Thr Leu Lys 
    1130                 1135                 1140             


Asp Phe  Trp Arg Met Val Trp  Glu Lys Asn Val Tyr  Ala Ile Ile 
    1145                 1150                 1155             


Met Leu  Thr Lys Cys Val Glu  Gln Gly Arg Thr Lys  Cys Glu Glu 
    1160                 1165                 1170             


Tyr Trp  Pro Ser Lys Gln Ala  Gln Asp Tyr Gly Asp  Ile Thr Val 
    1175                 1180                 1185             


Ala Met  Thr Ser Glu Ile Val  Leu Pro Glu Trp Thr  Ile Arg Asp 
    1190                 1195                 1200             


Phe Thr  Val Lys Asn Ile Gln  Thr Ser Glu Ser His  Pro Leu Arg 
    1205                 1210                 1215             


Gln Phe  His Phe Thr Ser Trp  Pro Asp His Gly Val  Pro Asp Thr 
    1220                 1225                 1230             


Thr Asp  Leu Leu Ile Asn Phe  Arg Tyr Leu Val Arg  Asp Tyr Met 
    1235                 1240                 1245             


Lys Gln  Ser Pro Pro Glu Ser  Pro Ile Leu Val His  Cys Ser Ala 
    1250                 1255                 1260             


Gly Val  Gly Arg Thr Gly Thr  Phe Ile Ala Ile Asp  Arg Leu Ile 
    1265                 1270                 1275             


Tyr Gln  Ile Glu Asn Glu Asn  Thr Val Asp Val Tyr  Gly Ile Val 
    1280                 1285                 1290             


Tyr Asp  Leu Arg Met His Arg  Pro Leu Met Val Gln  Thr Glu Asp 
    1295                 1300                 1305             


Gln Tyr  Val Phe Leu Asn Gln  Cys Val Leu Asp Ile  Val Arg Ser 
    1310                 1315                 1320             


Gln Lys  Asp Ser Lys Val Asp  Leu Ile Tyr Gln Asn  Thr Thr Ala 
    1325                 1330                 1335             


Met Thr  Ile Tyr Glu Asn Leu  Ala Pro Val Thr Thr  Phe Gly Lys 
    1340                 1345                 1350             


Thr Asn  Gly Tyr Ile Ala Ser  Gly Ser 
    1355                 1360         


<210>  51
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APRIL-based (A proliferation-inducing ligand-based) CAR, CD8 
       stalk APRIL CAR

<400>  51

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Val Leu His Leu Val Pro Ile Asn Ala Thr Ser 
            20                  25                  30          


Lys Asp Asp Ser Asp Val Thr Glu Val Met Trp Gln Pro Ala Leu Arg 
        35                  40                  45              


Arg Gly Arg Gly Leu Gln Ala Gln Gly Tyr Gly Val Arg Ile Gln Asp 
    50                  55                  60                  


Ala Gly Val Tyr Leu Leu Tyr Ser Gln Val Leu Phe Gln Asp Val Thr 
65                  70                  75                  80  


Phe Thr Met Gly Gln Val Val Ser Arg Glu Gly Gln Gly Arg Gln Glu 
                85                  90                  95      


Thr Leu Phe Arg Cys Ile Arg Ser Met Pro Ser His Pro Asp Arg Ala 
            100                 105                 110         


Tyr Asn Ser Cys Tyr Ser Ala Gly Val Phe His Leu His Gln Gly Asp 
        115                 120                 125             


Ile Leu Ser Val Ile Ile Pro Arg Ala Arg Ala Lys Leu Asn Leu Ser 
    130                 135                 140                 


Pro His Gly Thr Phe Leu Gly Phe Val Lys Leu Ser Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
                165                 170                 175     


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
            180                 185                 190         


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        195                 200                 205             


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
    210                 215                 220                 


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
225                 230                 235                 240 


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
                245                 250                 255     


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
            260                 265                 270         


Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro 
        275                 280                 285             


Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp 
    290                 295                 300                 


Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala 
305                 310                 315                 320 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
                325                 330                 335     


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
            340                 345                 350         


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
        355                 360                 365             


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
    370                 375                 380                 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
385                 390                 395                 400 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
                405                 410                 415     


His Met Gln Ala Leu Pro Pro Arg 
            420                 


<210>  52
<211>  398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APRIL-based (A proliferation-inducing ligand-based) CAR, APRIL 
       IgG1 hinge based CAR

<400>  52

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Val Leu His Leu Val Pro Ile Asn Ala Thr Ser 
            20                  25                  30          


Lys Asp Asp Ser Asp Val Thr Glu Val Met Trp Gln Pro Ala Leu Arg 
        35                  40                  45              


Arg Gly Arg Gly Leu Gln Ala Gln Gly Tyr Gly Val Arg Ile Gln Asp 
    50                  55                  60                  


Ala Gly Val Tyr Leu Leu Tyr Ser Gln Val Leu Phe Gln Asp Val Thr 
65                  70                  75                  80  


Phe Thr Met Gly Gln Val Val Ser Arg Glu Gly Gln Gly Arg Gln Glu 
                85                  90                  95      


Thr Leu Phe Arg Cys Ile Arg Ser Met Pro Ser His Pro Asp Arg Ala 
            100                 105                 110         


Tyr Asn Ser Cys Tyr Ser Ala Gly Val Phe His Leu His Gln Gly Asp 
        115                 120                 125             


Ile Leu Ser Val Ile Ile Pro Arg Ala Arg Ala Lys Leu Asn Leu Ser 
    130                 135                 140                 


Pro His Gly Thr Phe Leu Gly Phe Val Lys Leu Ser Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro 
                165                 170                 175     


Cys Pro Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val 
            180                 185                 190         


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
        195                 200                 205             


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
    210                 215                 220                 


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
225                 230                 235                 240 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro 
                245                 250                 255     


Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile 
            260                 265                 270         


Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val 
        275                 280                 285             


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
    290                 295                 300                 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
305                 310                 315                 320 


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
                325                 330                 335     


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
            340                 345                 350         


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
        355                 360                 365             


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
    370                 375                 380                 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
385                 390                 395             


<210>  53
<211>  614
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APRIL-based (A proliferation-inducing ligand-based) CAR, APRIL 
       Fc-pvaa based CAR

<400>  53

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Val Leu His Leu Val Pro Ile Asn Ala Thr Ser 
            20                  25                  30          


Lys Asp Asp Ser Asp Val Thr Glu Val Met Trp Gln Pro Ala Leu Arg 
        35                  40                  45              


Arg Gly Arg Gly Leu Gln Ala Gln Gly Tyr Gly Val Arg Ile Gln Asp 
    50                  55                  60                  


Ala Gly Val Tyr Leu Leu Tyr Ser Gln Val Leu Phe Gln Asp Val Thr 
65                  70                  75                  80  


Phe Thr Met Gly Gln Val Val Ser Arg Glu Gly Gln Gly Arg Gln Glu 
                85                  90                  95      


Thr Leu Phe Arg Cys Ile Arg Ser Met Pro Ser His Pro Asp Arg Ala 
            100                 105                 110         


Tyr Asn Ser Cys Tyr Ser Ala Gly Val Phe His Leu His Gln Gly Asp 
        115                 120                 125             


Ile Leu Ser Val Ile Ile Pro Arg Ala Arg Ala Lys Leu Asn Leu Ser 
    130                 135                 140                 


Pro His Gly Thr Phe Leu Gly Phe Val Lys Leu Ser Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro 
                165                 170                 175     


Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro 
            180                 185                 190         


Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys 
        195                 200                 205             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    210                 215                 220                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
225                 230                 235                 240 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                245                 250                 255     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            260                 265                 270         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        275                 280                 285             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    290                 295                 300                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
305                 310                 315                 320 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                325                 330                 335     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            340                 345                 350         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        355                 360                 365             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    370                 375                 380                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe Trp 
385                 390                 395                 400 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                405                 410                 415     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
            420                 425                 430         


Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
        435                 440                 445             


Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
    450                 455                 460                 


Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly 
465                 470                 475                 480 


Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His 
                485                 490                 495     


Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
            500                 505                 510         


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
        515                 520                 525             


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
    530                 535                 540                 


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
545                 550                 555                 560 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
                565                 570                 575     


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
            580                 585                 590         


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
        595                 600                 605             


Gln Ala Leu Pro Pro Arg 
    610                 


<210>  54
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AntiCD19: CD28TM-TCRz

<400>  54
ttttgggtgc tggtggtggt tggtggagtc ctggcttgct atagcttgct agtaacagtg       60

gcctttatta ttttctgggt gaggagagtg aagttcagca ggagcgcaga cgcccccgcg      120

taccagcagg gccagaacca gctctataac gagctcaatc taggacgaag agaggagtac      180

gatgttttgg acaagagacg tggccgggac cctgagatgg ggggaaagcc gagaaggaag      240

aaccctcagg aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt      300

gagattggga tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt      360

ctcagtacag ccaccaagga cacctacgac gcccttcaca tgcaggccct gcctcctcgc      420


<210>  55
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AntiCD33: CD28TM-TCRz

<400>  55
ttctgggtcc tggtggtggt gggaggcgtg ctggcctgtt actctctcct ggtgaccgtg       60

gccttcatca tcttttgggt gcgccgggtg aagttttctc gctctgccga tgccccagcc      120

tatcagcagg gccagaatca gctgtacaat gaactgaacc tgggcaggcg ggaggagtac      180

gacgtgctgg ataagcggag aggcagagac cccgagatgg gcggcaaacc acggcgcaaa      240

aatccccagg agggactcta taacgagctg cagaaggaca aaatggccga ggcctattcc      300

gagatcggca tgaagggaga gagaagacgc ggaaagggcc acgacggcct gtatcaggga      360

ttgtccaccg ctacaaaaga tacatatgat gccctgcaca tgcaggccct gccacccaga      420


