
                                SEQUENCE LISTING

<110> Frendewey, David
      Auerbach, Wojtek
      Lai, Ka-Man Venus
      Mujica, Alexander O.
      Lee, Jeffrey D.
      Droguett, Gustavo
      Trzaska, Sean
      Hunt, Charleen
      Gagliardi, Anthony
      Kuno, Junko
      Valenzuela, David M.
      Yancopoulos, George D.

<120> Methods and Compositions for the
  Targeted Modification of a Genome
  

<130> 057766/453460

<150> US 61/914,768       
<151> 2013-12-11  

<150> US 62/017,416       
<151> 2014-06-26  

<150> US 62/029,261       
<151> 2014-07-25  

<150> US 62/052,906       
<151> 2014-09-19  

<150> US 62/059,527       
<151> 2014-10-03  

<150> US 62/064,384 
<151> 2014-10-15  

<160> 94

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> a genomic target sequence that is linked to a
      guide RNA (gRNA)

<220> 
<221> misc_feature   
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,
19, 20, 21
<223> n = A,T,C or G

<400> 1
gnnnnnnnnn nnnnnnnnnn ngg                                         23

<210> 2
<211> 80
<212> RNA
<213> Artificial Sequence

<220> 
<223> a guide RNA (gRNA)

<400> 2
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu 60
ggcaccgagu cggugcuuuu                                             80

<210> 3
<211> 42
<212> RNA
<213> Artificial Sequence

<220> 
<223> a guide RNA (gRNA)

<400> 3
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cg                    42

<210> 4
<211> 30
<212> RNA
<213> Artificial Sequence

<220> 
<223> a crRNA

<400> 4
guuuuagagc uagaaauagc aaguuaaaau                                  30

<210> 5
<211> 33
<212> RNA
<213> Artificial Sequence

<220> 
<223> a crRNA

<400> 5
guuuuagagc uagaaauagc aaguuaaaau aag                              33

<210> 6
<211> 26
<212> RNA
<213> Artificial Sequence

<220> 
<223> a crRNA

<400> 6
gaguccgagc agaagaagaa guuuua                                      26

<210> 7
<211> 12
<212> RNA
<213> Artificial Sequence

<220> 
<223> a tracrRNA

<400> 7
aaggcuaguc cg                                                     12

<210> 8
<211> 50
<212> RNA
<213> Artificial Sequence

<220> 
<223> a tracrRNA

<400> 8
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu            50

<210> 9
<211> 203
<212> PRT
<213> Mus Musculus

<400> 9
Met Lys Val Leu Ala Ala Gly Ile Val Pro Leu Leu Leu Leu Val Leu
 1               5                  10                  15      
His Trp Lys His Gly Ala Gly Ser Pro Leu Pro Ile Thr Pro Val Asn
            20                  25                  30          
Ala Thr Cys Ala Ile Arg His Pro Cys His Gly Asn Leu Met Asn Gln
        35                  40                  45              
Ile Lys Asn Gln Leu Ala Gln Leu Asn Gly Ser Ala Asn Ala Leu Phe
    50                  55                  60                  
Ile Ser Tyr Tyr Thr Ala Gln Gly Glu Pro Phe Pro Asn Asn Val Glu
65                  70                  75                  80  
Lys Leu Cys Ala Pro Asn Met Thr Asp Phe Pro Ser Phe His Gly Asn
                85                  90                  95      
Gly Thr Glu Lys Thr Lys Leu Val Glu Leu Tyr Arg Met Val Ala Tyr
            100                 105                 110         
Leu Ser Ala Ser Leu Thr Asn Ile Thr Arg Asp Gln Lys Val Leu Asn
        115                 120                 125             
Pro Thr Ala Val Ser Leu Gln Val Lys Leu Asn Ala Thr Ile Asp Val
    130                 135                 140                 
Met Arg Gly Leu Leu Ser Asn Val Leu Cys Arg Leu Cys Asn Lys Tyr
145                 150                 155                 160 
Arg Val Gly His Val Asp Val Pro Pro Val Pro Asp His Ser Asp Lys
                165                 170                 175     
Glu Ala Phe Gln Arg Lys Lys Leu Gly Cys Gln Leu Leu Gly Thr Tyr
            180                 185                 190         
Lys Gln Val Ile Ser Val Val Val Gln Ala Phe
        195                 200             


<210> 10
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZFN1a binding site

<400> 10
caggccctga accgc                                                  15

<210> 11
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZFN1 cutting site

<400> 11
ttctgg                                                            6

<210> 12
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZFN1b binding  site

<400> 12
gattacctgc gctggg                                                 16

<210> 13
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZF21a binding  site

<400> 13
ttcaccctcc gcacc                                                  15

<210> 14
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZFN2 cutting  site

<400> 14
tgctgag                                                           7

<210> 15
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZF21b binding site

<400> 15
tatccagatc caggggtt                                               18

<210> 16
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> conserved domain of a family of homing nucleases

<400> 16
Leu Ala Gly Leu Ile Asp Ala Asp Gly
 1               5                  


<210> 17
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZFN1a binding site

<400> 17
caggccctga accgc                                                  15

<210> 18
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZFN1 cutting site

<400> 18
ttctgg                                                            6

<210> 19
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> ZFN1b binding site

<400> 19
gattacctgc gctggg                                                 16

<210> 20
<211> 369
<212> PRT
<213> Homo sapiens

<400> 20
Met Leu Lys Pro Ser Leu Pro Phe Thr Ser Leu Leu Phe Leu Gln Leu
 1               5                  10                  15      
Pro Leu Leu Gly Val Gly Leu Asn Thr Thr Ile Leu Thr Pro Asn Gly
            20                  25                  30          
Asn Glu Asp Thr Thr Ala Asp Phe Phe Leu Thr Thr Met Pro Thr Asp
        35                  40                  45              
Ser Leu Ser Val Ser Thr Leu Pro Leu Pro Glu Val Gln Cys Phe Val
    50                  55                  60                  
Phe Asn Val Glu Tyr Met Asn Cys Thr Trp Asn Ser Ser Ser Glu Pro
65                  70                  75                  80  
Gln Pro Thr Asn Leu Thr Leu His Tyr Trp Tyr Lys Asn Ser Asp Asn
                85                  90                  95      
Asp Lys Val Gln Lys Cys Ser His Tyr Leu Phe Ser Glu Glu Ile Thr
            100                 105                 110         
Ser Gly Cys Gln Leu Gln Lys Lys Glu Ile His Leu Tyr Gln Thr Phe
        115                 120                 125             
Val Val Gln Leu Gln Asp Pro Arg Glu Pro Arg Arg Gln Ala Thr Gln
    130                 135                 140                 
Met Leu Lys Leu Gln Asn Leu Val Ile Pro Trp Ala Pro Glu Asn Leu
145                 150                 155                 160 
Thr Leu His Lys Leu Ser Glu Ser Gln Leu Glu Leu Asn Trp Asn Asn
                165                 170                 175     
Arg Phe Leu Asn His Cys Leu Glu His Leu Val Gln Tyr Arg Thr Asp
            180                 185                 190         
Trp Asp His Ser Trp Thr Glu Gln Ser Val Asp Tyr Arg His Lys Phe
        195                 200                 205             
Ser Leu Pro Ser Val Asp Gly Gln Lys Arg Tyr Thr Phe Arg Val Arg
    210                 215                 220                 
Ser Arg Phe Asn Pro Leu Cys Gly Ser Ala Gln His Trp Ser Glu Trp
225                 230                 235                 240 
Ser His Pro Ile His Trp Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe
                245                 250                 255     
Leu Phe Ala Leu Glu Ala Val Val Ile Ser Val Gly Ser Met Gly Leu
            260                 265                 270         
Ile Ile Ser Leu Leu Cys Val Tyr Phe Trp Leu Glu Arg Thr Met Pro
        275                 280                 285             
Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His
    290                 295                 300                 
Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys Gly Leu Ala Glu Ser
305                 310                 315                 320 
Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu Val Ser Glu Ile Pro
                325                 330                 335     
Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn
            340                 345                 350         
Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu
        355                 360                 365             
Thr
    


<210> 21
<211> 368
<212> PRT
<213> Rattus norvegicus

<400> 21
Met Leu Lys Pro Leu Leu Pro Ser Arg Ser Phe Leu Leu Leu Gln Leu
 1               5                  10                  15      
Leu Leu Leu Arg Val Gly Trp Ser Ser Lys Val Leu Met Ser Ser Gly
            20                  25                  30          
Asn Glu Asp Thr Lys Ser Asp Leu Leu Leu Thr Ser Met Asp Leu Lys
        35                  40                  45              
His Leu Ser Val Pro Thr Leu Pro Leu Pro Glu Val Gln Cys Phe Val
    50                  55                  60                  
Phe Asn Val Glu Tyr Met Asn Cys Thr Trp Asn Ser Ser Ser Glu Pro
65                  70                  75                  80  
Gln Pro Thr Asn Leu Thr Met His Tyr Arg Tyr Lys Gly Ser Asp Asn
                85                  90                  95      
Asn Thr Phe Gln Glu Cys Ser His Tyr Leu Phe Ser Lys Glu Ile Thr
            100                 105                 110         
Ser Gly Cys Gln Ile Gln Lys Glu Asp Ile Gln Leu Tyr Gln Thr Phe
        115                 120                 125             
Val Val Gln Leu Gln Asp Pro Gln Lys Pro Gln Arg Arg Ala Glu Gln
    130                 135                 140                 
Lys Leu Asn Leu Gln Asn Leu Val Ile Pro Trp Ala Pro Glu Asn Leu
145                 150                 155                 160 
Thr Leu Tyr Asn Leu Ser Glu Ser Gln Val Glu Leu Arg Trp Lys Ser
                165                 170                 175     
Arg Tyr Ile Glu Arg Cys Leu Gln Tyr Leu Val Gln Tyr Arg Ser Asn
            180                 185                 190         
Arg Asp Arg Ser Trp Thr Glu Gln Ile Val Asp His Glu Pro Arg Phe
        195                 200                 205             
Ser Leu Pro Ser Val Asp Glu Gln Lys Leu Tyr Thr Phe Arg Val Arg
    210                 215                 220                 
Ser Arg Phe Asn Pro Ile Cys Gly Ser Thr Gln Gln Trp Ser Lys Trp
225                 230                 235                 240 
Ser Gln Pro Ile His Trp Gly Ser His Thr Ala Glu Glu Asn Pro Ser
                245                 250                 255     
Leu Phe Ala Leu Glu Ala Val Leu Ile Pro Val Gly Thr Met Gly Leu
            260                 265                 270         
Ile Ile Thr Leu Ile Phe Val Tyr Cys Trp Leu Glu Arg Met Pro Arg
        275                 280                 285             
Ile Pro Ala Ile Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly
    290                 295                 300                 
Asn Phe Ser Ala Trp Ser Gly Val Ser Lys Gly Leu Thr Glu Ser Leu
305                 310                 315                 320 
Gln Pro Asp Tyr Ser Glu Arg Phe Cys His Val Ser Glu Ile Pro Pro
                325                 330                 335     
Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly Gly Ser Pro Cys Ser Leu
            340                 345                 350         
His Ser Pro Tyr Trp Pro Pro Pro Cys Tyr Ser Leu Lys Pro Glu Ala
        355                 360                 365             


<210> 22
<211> 368
<212> PRT
<213> Artificial Sequence

<220> 
<223> chimeric IL-2 receptor gamma comprising the rat
      IL-2 receptor gamma protein having the ecto domain
      of IL-2 gamma receptor from human

<400> 22
Met Leu Lys Pro Ser Leu Pro Phe Thr Ser Leu Leu Phe Leu Gln Leu
 1               5                  10                  15      
Pro Leu Leu Gly Val Gly Leu Asn Thr Thr Ile Leu Thr Pro Asn Gly
            20                  25                  30          
Asn Glu Asp Thr Thr Ala Asp Phe Phe Leu Thr Thr Met Pro Thr Asp
        35                  40                  45              
Ser Leu Ser Val Ser Thr Leu Pro Leu Pro Glu Val Gln Cys Phe Val
    50                  55                  60                  
Phe Asn Val Glu Tyr Met Asn Cys Thr Trp Asn Ser Ser Ser Glu Pro
65                  70                  75                  80  
Gln Pro Thr Asn Leu Thr Leu His Tyr Trp Tyr Lys Asn Ser Asp Asn
                85                  90                  95      
Asp Lys Val Gln Lys Cys Ser His Tyr Leu Phe Ser Glu Glu Ile Thr
            100                 105                 110         
Ser Gly Cys Gln Leu Gln Lys Lys Glu Ile His Leu Tyr Gln Thr Phe
        115                 120                 125             
Val Val Gln Leu Gln Asp Pro Arg Glu Pro Arg Arg Gln Ala Thr Gln
    130                 135                 140                 
Met Leu Lys Leu Gln Asn Leu Val Ile Pro Trp Ala Pro Glu Asn Leu
145                 150                 155                 160 
Thr Leu His Lys Leu Ser Glu Ser Gln Leu Glu Leu Asn Trp Asn Asn
                165                 170                 175     
Arg Phe Leu Asn His Cys Leu Glu His Leu Val Gln Tyr Arg Thr Asp
            180                 185                 190         
Trp Asp His Ser Trp Thr Glu Gln Ser Val Asp Tyr Arg His Lys Phe
        195                 200                 205             
Ser Leu Pro Ser Val Asp Gly Gln Lys Arg Tyr Thr Phe Arg Val Arg
    210                 215                 220                 
Ser Arg Phe Asn Pro Leu Cys Gly Ser Ala Gln His Trp Ser Glu Trp
225                 230                 235                 240 
Ser His Pro Ile His Trp Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe
                245                 250                 255     
Leu Phe Ala Leu Glu Ala Val Leu Ile Pro Val Gly Thr Met Gly Leu
            260                 265                 270         
Ile Ile Thr Leu Ile Phe Val Tyr Cys Trp Leu Glu Arg Met Pro Arg
        275                 280                 285             
Ile Pro Ala Ile Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly
    290                 295                 300                 
Asn Phe Ser Ala Trp Ser Gly Val Ser Lys Gly Leu Thr Glu Ser Leu
305                 310                 315                 320 
Gln Pro Asp Tyr Ser Glu Arg Phe Cys His Val Ser Glu Ile Pro Pro
                325                 330                 335     
Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly Gly Ser Pro Cys Ser Leu
            340                 345                 350         
His Ser Pro Tyr Trp Pro Pro Pro Cys Tyr Ser Leu Lys Pro Glu Ala
        355                 360                 365             


<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> a genomic target sequence that is linked to a
      guide RNA (gRNA)

<220> 
<221> misc_feature   
<222> (2)...(21)
<223> n= A, T, C or G

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> n can be from 1-20 nucleotides

<400> 23
gnnnnnnnnn nnnnnnnnnn ngg                                         23

<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 24
gggaacccac agcatactcc                                             20

<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 25
gaatcatgca cggctacccc                                             20

<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 26
tgctcctatg gggaggcgcg                                             20

<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 27
cttggataac attgataccc                                             20

<210> 28
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 28
ggggcagagc ccttatatca                                             20

<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 29
tcgctcacat taatccctag                                             20

<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 30
gtactgggga atcggtggtc                                             20

<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 31
cacgcactcc aaatttatcc                                             20

<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 32
ctaagtgtgt atcagtacat                                             20

<210> 33
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 33
tgccctgcac aataagcgca                                             20

<210> 34
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 34
actcattgaa acgttatggc                                             20

<210> 35
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 35
agtaagggtg gattaaattc                                             20

<210> 36
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 36
gccatctaga ttcatgtaac                                             20

<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 37
gactagaaat gttctgcacc                                             20

<210> 38
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 38
tgaaccaatt gtgtagcctt                                             20

<210> 39
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 39
aatagtggta aagcaccatg                                             20

<210> 40
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 40
gtgtgctaag gatcgaagtc                                             20

<210> 41
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 41
caccgagatg cttgggtatt                                             20

<210> 42
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 42
tgtaaccgcc ctgaatgacc                                             20

<210> 43
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 43
aaaagggcat cataaatccc                                             20

<210> 44
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 44
tcaaaaatag tcatacacct                                             20

<210> 45
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 45
ggtctctagt acattgtaga                                             20

<210> 46
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 46
atcacaaacc agttaaccgg                                             20

<210> 47
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 47
tttcagacga gccgacccgg                                             20

<210> 48
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 48
ctgtcaacag tgccgcgttt                                             20

<210> 49
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 49
tgtgtgtcat agcgatgtcg                                             20

<210> 50
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 50
aacaggtacc ctatcctcac                                             20

<210> 51
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 51
tcgtggttgc atgcgcactg                                             20

<210> 52
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 52
ggcccggacc tagtctctct                                             20

<210> 53
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 53
agtctgtaaa gttagcagtc                                             20

<210> 54
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 54
ggtggtggtg ctgacggaca                                             20

<210> 55
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 55
tatgagatca acactcgcta                                             20

<210> 56
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 56
ccaaggactt ccccacgtta                                             20

<210> 57
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 57
tgcttccctt atgcaagatt                                             20

<210> 58
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 58
ttaggtaccc tatttgaata                                             20

<210> 59
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 59
tgcagtgggt gacaggtcca                                             20

<210> 60
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 60
agggttatac tgacgttgtg                                             20

<210> 61
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 61
tgtctttcaa ggagggctac                                             20

<210> 62
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 62
tgatgtgcag tcagacaaag                                             20

<210> 63
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 63
tgcactatgg ttgactatga                                             20

<210> 64
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 64
ggaatattct aataggaagt                                             20

<210> 65
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 65
aagtgctgta ccattctagc                                             20

<210> 66
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 66
taatcaatag acaacctcgt                                             20

<210> 67
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 67
tcattcctaa tggtattata                                             20

<210> 68
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 68
agggtacata gatggcatcg                                             20

<210> 69
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 69
ctctttaaca attaccactt                                             20

<210> 70
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 70
tgtgggcctt tgctgatcac                                             20

<210> 71
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 71
aatctatgat cctatggcct                                             20

<210> 72
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 72
tgccaatagc agtgacttga                                             20

<210> 73
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 73
gggaagaatg ggctattgtc                                             20

<210> 74
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 74
ggttgtttgt gctgatgacg                                             20

<210> 75
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 75
ccgtcctagg ccttctacgt                                             20

<210> 76
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 76
actagtagac ctgaggggtt                                             20

<210> 77
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 77
gctccagtgt ttaggccttg                                             20

<210> 78
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 78
ggcaagctga aaacgcatgc                                             20

<210> 79
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 79
gtagatcgct ttccactacc                                             20

<210> 80
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 80
gaactccact gctcgtgagc                                             20

<210> 81
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 81
ataggtgggc actattgaag                                             20

<210> 82
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 82
atgggaaggt ttataccagc                                             20

<210> 83
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 83
cggtgtaaaa acaacgggaa                                             20

<210> 84
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 84
gacccgcagu cccagcgucg                                             20

<210> 85
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 85
actgagatca atgaccccga                                             20

<210> 86
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 86
gggtcgcccg gaacctctac                                             20

<210> 87
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 87
gcaggccctg aaccgcttct tgg                                         23

<210> 88
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 88
cctgcgctgg gtgcagacgc ttt                                         23

<210> 89
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 89
ccagctactt gctcgtacaa                                             20

<210> 90
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 90
cccctcagat tcacgtgcgt                                             20

<210> 91
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 91
gaagctcttt ctatacaatc tgg                                         23

<210> 92
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> a guide sequence

<400> 92
cccccgaaag gaggagccct agg                                         23

<210> 93
<211> 56
<212> DNA
<213> Rattus norvegicus

<400> 93
ttctgagcct cagccgacca acctcactat gcactatagg tatgagaagg gggagg     56

<210> 94
<211> 20
<212> DNA
<213> aArtificial Sequence

<220> 
<223> a guide sequence

<400> 94
gtatagccct gttacacatt                                             20
