                         SEQUENCE LISTING

<110>  DOW Global Technologies Inc.
       Sanghani, Paresh
       Stowers, Christopher
       Rodriguez, Brandon
       Venkateswaran, Amudhan
 
<120>  PROCESSES TO PREPARE ELONGATED 2-KETOACIDS AND C6-C10 COMPOUNDS 
       THEREFROM VIA GENETIC MODIFICATIONS TO MICROBIAL METABOLIC PATHWAYS 
       

<130>  75413-WO-PCT

<150>  US 61/915,040
<151>  2013-12-12

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  1089
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  Wild type E.coli LeuB

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<400>  1
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg ctg        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Leu           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac gtg ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  2
<211>  363
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Leu 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  3
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:2 L96A

<220>
<221>  CDS
<222>  (1)..(1089)

<400>  3
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg gcc        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Ala           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac gtg ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  4
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  4

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Ala 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  5
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:3 L96V

<400>  5
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg gtg        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Val           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac gtg ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  6
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  6

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Val 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  7
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:4 L96G

<400>  7
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg ggt        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Gly           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac gtg ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  8
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  8

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Gly 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Val Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  9
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:5 V198A

<400>  9
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg ctg        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Leu           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac gcg ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Ala Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  10
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  10

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Leu 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Ala Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  11
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:6 V198G

<400>  11
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg ctg        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Leu           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac ggt ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Gly Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  12
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  12

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Leu 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Gly Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  13
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:7 L96A/V198A

<400>  13
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg gcc        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Ala           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac gcc ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Ala Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  14
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  14

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Ala 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Ala Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  15
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:8 L96G/V198A

<400>  15
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg ggt        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Gly           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac gcg ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Ala Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  16
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  16

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Gly 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Ala Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  17
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:9 L96G/V198G

<400>  17
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg ggt        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Gly           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac ggt ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Gly Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  18
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  18

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Gly 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Gly Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


<210>  19
<211>  1089
<212>  DNA
<213>  ENGINEERED PROTEIN


<220>
<221>  CDS
<222>  (1)..(1089)

<220>
<221>  mat_peptide
<222>  (1)..(1089)

<220>
<221>  gene
<222>  (1)..(1089)
<223>  SEQ ID NO:10 L96V/V198G

<400>  19
atg tcg aag aat tac cat att gcc gta ttg ccg ggg gac ggt att ggt         48
Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly           
1               5                   10                  15                

ccg gaa gtg atg acc cag gcg ctg aaa gtg ctg gat gcc gtg cgc aac         96
Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn           
            20                  25                  30                    

cgc ttt gcg atg cgc atc acc acc agc cat tac gat gta ggc ggc gca        144
Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala           
        35                  40                  45                        

gcc att gat aac cac ggg caa cca ctg ccg cct gcg acg gtt gaa ggt        192
Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly           
    50                  55                  60                            

tgt gag caa gcc gat gcc gtg ctg ttt ggc tcg gta ggc ggc ccg aag        240
Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys           
65                  70                  75                  80            

tgg gaa cat tta cca cca gac cag caa cca gaa cgc ggc gcg ctg gtg        288
Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Val           
                85                  90                  95                

cct ctg cgt aag cac ttc aaa tta ttc agc aac ctg cgc ccg gca aaa        336
Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys           
            100                 105                 110                   

ctg tat cag ggg ctg gaa gca ttc tgt ccg ctg cgt gca gac att gcc        384
Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala           
        115                 120                 125                       

gca aac ggc ttc gac atc ctg tgt gtg cgc gaa ctg acc ggc ggc atc        432
Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile           
    130                 135                 140                           

tat ttc ggt cag cca aaa ggc cgc gaa ggt agc gga caa tat gaa aaa        480
Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys           
145                 150                 155                 160           

gcc ttt gat acc gag gtg tat cac cgt ttt gag atc gaa cgt atc gcc        528
Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala           
                165                 170                 175               

cgc atc gcg ttt gaa tct gct cgc aag cgt cgc cac aaa gtg acg tcg        576
Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser           
            180                 185                 190                   

atc gat aaa gcc aac ggt ctg caa tcc tct att tta tgg cgg gag atc        624
Ile Asp Lys Ala Asn Gly Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile           
        195                 200                 205                       

gtt aac gag atc gcc acg gaa tac ccg gat gtc gaa ctg gcg cac atg        672
Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met           
    210                 215                 220                           

tac atc gac aac gcc acc atg cag ctg att aaa gat cca tca cag ttt        720
Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe           
225                 230                 235                 240           

gac gtt ctg ctg tgc tcc aac ctg ttt ggc gac att ctg tct gac gag        768
Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu           
                245                 250                 255               

tgc gca atg atc act ggc tcg atg ggg atg ttg cct tcc gcc agc ctg        816
Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu           
            260                 265                 270                   

aac gag caa ggt ttt gga ctg tat gaa ccg gcg ggc ggc tcg gca cca        864
Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro           
        275                 280                 285                       

gat atc gca ggc aaa aac atc gcc aac ccg att gca caa atc ctt tcg        912
Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser           
    290                 295                 300                           

ctg gca ctg ctg ctg cgt tac agc ctg gat gcc gat gat gcg gct tgc        960
Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys           
305                 310                 315                 320           

gcc att gaa cgc gcc att aac cgc gca tta gaa gaa ggc att cgc acc       1008
Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr           
                325                 330                 335               

ggg gat tta gcc cgt ggc gct gcc gcc gtt agt acc gat gaa atg ggc       1056
Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly           
            340                 345                 350                   

gat atc att gcc cgc tat gta gca gaa ggg gtg                           1089
Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val                               
        355                 360                                           


<210>  20
<211>  363
<212>  PRT
<213>  ENGINEERED PROTEIN

<400>  20

Met Ser Lys Asn Tyr His Ile Ala Val Leu Pro Gly Asp Gly Ile Gly 
1               5                   10                  15      


Pro Glu Val Met Thr Gln Ala Leu Lys Val Leu Asp Ala Val Arg Asn 
            20                  25                  30          


Arg Phe Ala Met Arg Ile Thr Thr Ser His Tyr Asp Val Gly Gly Ala 
        35                  40                  45              


Ala Ile Asp Asn His Gly Gln Pro Leu Pro Pro Ala Thr Val Glu Gly 
    50                  55                  60                  


Cys Glu Gln Ala Asp Ala Val Leu Phe Gly Ser Val Gly Gly Pro Lys 
65                  70                  75                  80  


Trp Glu His Leu Pro Pro Asp Gln Gln Pro Glu Arg Gly Ala Leu Val 
                85                  90                  95      


Pro Leu Arg Lys His Phe Lys Leu Phe Ser Asn Leu Arg Pro Ala Lys 
            100                 105                 110         


Leu Tyr Gln Gly Leu Glu Ala Phe Cys Pro Leu Arg Ala Asp Ile Ala 
        115                 120                 125             


Ala Asn Gly Phe Asp Ile Leu Cys Val Arg Glu Leu Thr Gly Gly Ile 
    130                 135                 140                 


Tyr Phe Gly Gln Pro Lys Gly Arg Glu Gly Ser Gly Gln Tyr Glu Lys 
145                 150                 155                 160 


Ala Phe Asp Thr Glu Val Tyr His Arg Phe Glu Ile Glu Arg Ile Ala 
                165                 170                 175     


Arg Ile Ala Phe Glu Ser Ala Arg Lys Arg Arg His Lys Val Thr Ser 
            180                 185                 190         


Ile Asp Lys Ala Asn Gly Leu Gln Ser Ser Ile Leu Trp Arg Glu Ile 
        195                 200                 205             


Val Asn Glu Ile Ala Thr Glu Tyr Pro Asp Val Glu Leu Ala His Met 
    210                 215                 220                 


Tyr Ile Asp Asn Ala Thr Met Gln Leu Ile Lys Asp Pro Ser Gln Phe 
225                 230                 235                 240 


Asp Val Leu Leu Cys Ser Asn Leu Phe Gly Asp Ile Leu Ser Asp Glu 
                245                 250                 255     


Cys Ala Met Ile Thr Gly Ser Met Gly Met Leu Pro Ser Ala Ser Leu 
            260                 265                 270         


Asn Glu Gln Gly Phe Gly Leu Tyr Glu Pro Ala Gly Gly Ser Ala Pro 
        275                 280                 285             


Asp Ile Ala Gly Lys Asn Ile Ala Asn Pro Ile Ala Gln Ile Leu Ser 
    290                 295                 300                 


Leu Ala Leu Leu Leu Arg Tyr Ser Leu Asp Ala Asp Asp Ala Ala Cys 
305                 310                 315                 320 


Ala Ile Glu Arg Ala Ile Asn Arg Ala Leu Glu Glu Gly Ile Arg Thr 
                325                 330                 335     


Gly Asp Leu Ala Arg Gly Ala Ala Ala Val Ser Thr Asp Glu Met Gly 
            340                 345                 350         


Asp Ile Ile Ala Arg Tyr Val Ala Glu Gly Val 
        355                 360             


