                         SEQUENCE LISTING

<110>  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
       WEINER, David
       MUTHUMANI, Karupiah
       WALTERS, Jewell
       SARDESAI, Niranjan
 
<120>  DNA ANTIBODY CONSTRUCTS AND METHOD OF USING SAME

<130>  UPVG0053 WO2

<150>  PCT/US13/075137
<151>  2013-12-13

<160>  78    

<170>  PatentIn version 3.5

<210>  1
<211>  738
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of HIV-1 Env-4E10 Ig

<400>  1

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Arg 
            20                  25                  30          


Pro Gly Ser Ser Val Thr Val Ser Cys Lys Ala Ser Gly Gly Ser Phe 
        35                  40                  45              


Ser Thr Tyr Ala Leu Ser Trp Val Arg Gln Ala Pro Gly Arg Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Gly Val Ile Pro Leu Leu Thr Ile Thr Asn Tyr Ala 
65                  70                  75                  80  


Pro Arg Phe Gln Gly Arg Ile Thr Ile Thr Ala Asp Arg Ser Thr Ser 
                85                  90                  95      


Thr Ala Tyr Leu Glu Leu Asn Ser Leu Arg Pro Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Glu Gly Thr Thr Gly Trp Gly Trp Leu Gly Lys 
        115                 120                 125             


Pro Ile Gly Ala Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val 
    130                 135                 140                 


Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
145                 150                 155                 160 


Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys 
                165                 170                 175     


Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
            180                 185                 190         


Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
        195                 200                 205             


Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
    210                 215                 220                 


Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
225                 230                 235                 240 


Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
                245                 250                 255     


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
            260                 265                 270         


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
        275                 280                 285             


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
    290                 295                 300                 


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
305                 310                 315                 320 


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
                325                 330                 335     


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
            340                 345                 350         


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
        355                 360                 365             


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
    370                 375                 380                 


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
385                 390                 395                 400 


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
                405                 410                 415     


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
            420                 425                 430         


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
        435                 440                 445             


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
    450                 455                 460                 


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys 
465                 470                 475                 480 


Arg Arg Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala 
                485                 490                 495     


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Leu Gln Thr Gln Val 
            500                 505                 510         


Phe Ile Ser Leu Leu Leu Trp Ile Ser Gly Ala Tyr Gly Glu Ile Val 
        515                 520                 525             


Leu Thr Gln Ser Pro Gly Thr Gln Ser Leu Ser Pro Gly Glu Arg Ala 
    530                 535                 540                 


Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Asn Asn Lys Leu Ala 
545                 550                 555                 560 


Trp Tyr Gln Gln Arg Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Gly 
                565                 570                 575     


Ala Ser Ser Arg Pro Ser Gly Val Ala Asp Arg Phe Ser Gly Ser Gly 
            580                 585                 590         


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp 
        595                 600                 605             


Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Gln Ser Leu Ser Thr Phe 
    610                 615                 620                 


Gly Gln Gly Thr Lys Val Glu Lys Arg Thr Val Ala Ala Pro Ser Val 
625                 630                 635                 640 


Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser 
                645                 650                 655     


Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln 
            660                 665                 670         


Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val 
        675                 680                 685             


Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu 
    690                 695                 700                 


Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu 
705                 710                 715                 720 


Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg 
                725                 730                 735     


Gly Glu 
        


<210>  2
<211>  768
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of HIV-1 Env-PG9 Ig

<400>  2

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Phe Leu 
            20                  25                  30          


Arg Gly Val Gln Cys Gln Arg Leu Val Glu Ser Gly Gly Gly Val Val 
        35                  40                  45              


Gln Pro Gly Ser Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp 
    50                  55                  60                  


Phe Ser Arg Gln Gly Met His Trp Val Arg Gln Ala Pro Gly Gln Gly 
65                  70                  75                  80  


Leu Glu Trp Val Ala Phe Ile Lys Tyr Asp Gly Ser Glu Lys Tyr His 
                85                  90                  95      


Ala Asp Ser Val Trp Gly Arg Leu Ser Ile Ser Arg Asp Asn Ser Lys 
            100                 105                 110         


Asp Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala 
        115                 120                 125             


Thr Tyr Phe Cys Val Arg Glu Ala Gly Gly Pro Asp Tyr Arg Asn Gly 
    130                 135                 140                 


Tyr Asn Tyr Tyr Asp Phe Tyr Asp Gly Tyr Tyr Asn Tyr His Tyr Met 
145                 150                 155                 160 


Asp Val Trp Gly Lys Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr 
                165                 170                 175     


Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
            180                 185                 190         


Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
        195                 200                 205             


Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
    210                 215                 220                 


Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
225                 230                 235                 240 


Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
                245                 250                 255     


Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu 
            260                 265                 270         


Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
        275                 280                 285             


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
    290                 295                 300                 


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
305                 310                 315                 320 


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
                325                 330                 335     


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
            340                 345                 350         


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
        355                 360                 365             


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
    370                 375                 380                 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
385                 390                 395                 400 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
                405                 410                 415     


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
            420                 425                 430         


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
        435                 440                 445             


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
    450                 455                 460                 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
465                 470                 475                 480 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
                485                 490                 495     


Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser Gly Ser 
            500                 505                 510         


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
        515                 520                 525             


Asn Pro Gly Pro Met Ala Trp Thr Pro Leu Phe Leu Phe Leu Leu Thr 
    530                 535                 540                 


Cys Cys Pro Gly Gly Ser Asn Ser Gln Ser Ala Leu Thr Gln Pro Ala 
545                 550                 555                 560 


Ser Val Ser Gly Ser Pro Gly Gln Ser Ile Thr Ile Ser Cys Asn Gly 
                565                 570                 575     


Thr Ser Asn Asp Val Gly Gly Tyr Glu Ser Val Ser Trp Tyr Gln Gln 
            580                 585                 590         


His Pro Gly Lys Ala Pro Lys Val Val Ile Tyr Asp Val Ser Lys Arg 
        595                 600                 605             


Pro Ser Gly Val Ser Asn Arg Phe Ser Gly Ser Lys Ser Gly Asn Thr 
    610                 615                 620                 


Ala Ser Leu Thr Ile Ser Gly Leu Gln Ala Glu Asp Glu Gly Asp Tyr 
625                 630                 635                 640 


Tyr Cys Lys Ser Leu Thr Ser Thr Arg Arg Arg Val Phe Gly Thr Gly 
                645                 650                 655     


Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro Ser Val Thr 
            660                 665                 670         


Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu 
        675                 680                 685             


Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr Val Ala Trp 
    690                 695                 700                 


Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr Thr Thr Pro 
705                 710                 715                 720 


Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu 
                725                 730                 735     


Thr Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys Gln Val Thr 
            740                 745                 750         


His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
        755                 760                 765             


<210>  3
<211>  792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding the Heavy Chain (VH-CH1) of HIV-1 
       Env Fab

<400>  3
aagcttgccg ccaccatgga gactgataca ctgctgctgt gggtgctgct gctgtgggtg       60

ccagggtcaa ccggagatgg ggctcaggtc cagctggtcc agagcggcgg acagatgaag      120

aaacccggcg agagcatgag gatctcctgc agagcatctg gatacgagtt catcgactgt      180

accctgaact ggattaggct ggctcctgga aagagaccag agtggatggg gtggctgaaa      240

ccacgagggg gagcagtgaa ttacgcccgg cccctgcagg gacgagtgac catgaccagg      300

gacgtgtaca gcgataccgc cttcctggag ctgcggtccc tgacagtgga cgatactgct      360

gtctacttct gcacacgcgg aaagaactgt gactataatt gggattttga acactggggc      420

cggggaacac ccgtgatcgt cagctccccc agtactaagg gaccttcagt gtttccactg      480

gccccctcta gtaaatccac ctctggaggg acagccgctc tgggatgcct ggtgaaagat      540

tatttccccg aacctgtgac cgtcagttgg aactcagggg ctctgacttc tggcgtgcac      600

acctttcctg cagtcctgca gtcaagcggg ctgtacagtc tgtcctctgt ggtcactgtg      660

cctagttcaa gcctgggcac tcagacctat atttgtaacg tgaatcataa gccatccaat      720

acaaaagtgg acaaaaaagc cgaacccaaa tcctgttacc cttatgatgt gcccgactac      780

gcctgactcg ag                                                          792


<210>  4
<211>  756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Light Chain (VL-CL) of HIV-1 Env Fab

<400>  4
aagcttgccg ccaccatgga aaccgataca ctgctgctgt gggtgctgct gctgtgggtg       60

ccaggaagta ccggggatgg ggctcaggtc cagattgtgc tgactcagtc ccctgggacc      120

ctgtctctga gtccaggcga gacagctatc atttcatgcc gaactagcca gtacggcagc      180

ctggcttggt atcagcagcg accaggacag gcaccacgac tggtcatcta ctcaggcagc      240

acaagggccg ctggcatccc cgacaggttc tccggcagca ggtgggggcc tgattacaac      300

ctgactatct ctaatctgga gagtggggac tttggcgtgt actattgcca gcagtatgag      360

ttcttcggcc agggaactaa ggtgcaggtg gacatcaaaa gaaccgtggc agccccatcc      420

gtcttcattt ttcccccttc tgatgagcag ctgaagtcag gcaccgccag cgtggtctgt      480

ctgctgaaca atttctaccc ccgggaagcc aaggtgcagt ggaaagtgga caacgctctg      540

cagagtggaa attcacagga gagcgtgacc gaacaggact ccaaggattc tacatatagt      600

ctgagcagca ccctgaccct gagtaaagca gattacgaga agcacaaagt gtatgcctgt      660

gaagtcacac atcagggcct gaggagcccc gtgactaaaa gtttcaaccg aggagagtgc      720

tacccttatg atgtgcccga ctacgcctaa ctcgag                                756


<210>  5
<211>  731
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VRC01 IgG

<400>  5

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Gln Ser Gly Gly Gln Met Lys Lys Pro 
            20                  25                  30          


Gly Glu Ser Met Arg Ile Ser Cys Arg Ala Ser Gly Tyr Glu Phe Ile 
        35                  40                  45              


Asp Cys Thr Leu Asn Trp Ile Arg Leu Ala Pro Gly Lys Arg Pro Glu 
    50                  55                  60                  


Trp Met Gly Trp Leu Lys Pro Arg Gly Gly Ala Val Asn Tyr Ala Arg 
65                  70                  75                  80  


Pro Leu Gln Gly Arg Val Thr Met Thr Arg Asp Val Tyr Ser Asp Thr 
                85                  90                  95      


Ala Phe Leu Glu Leu Arg Ser Leu Thr Val Asp Asp Thr Ala Val Tyr 
            100                 105                 110         


Phe Cys Thr Arg Gly Lys Asn Cys Asp Tyr Asn Trp Asp Phe Glu His 
        115                 120                 125             


Trp Gly Arg Gly Thr Pro Val Ile Val Ser Ser Pro Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Ala Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            260                 265                 270         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        275                 280                 285             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    290                 295                 300                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
305                 310                 315                 320 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                325                 330                 335     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            340                 345                 350         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        355                 360                 365             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    370                 375                 380                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
385                 390                 395                 400 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                405                 410                 415     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            420                 425                 430         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        435                 440                 445             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    450                 455                 460                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg 
465                 470                 475                 480 


Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
                485                 490                 495     


Val Glu Glu Asn Pro Gly Pro Met Asp Trp Thr Trp Ile Leu Phe Leu 
            500                 505                 510         


Val Ala Ala Ala Thr Arg Val His Ser Glu Ile Val Leu Thr Gln Ser 
        515                 520                 525             


Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Thr Ala Ile Ile Ser Cys 
    530                 535                 540                 


Arg Thr Ser Gln Tyr Gly Ser Leu Ala Trp Tyr Gln Gln Arg Pro Gly 
545                 550                 555                 560 


Gln Ala Pro Arg Leu Val Ile Tyr Ser Gly Ser Thr Arg Ala Ala Gly 
                565                 570                 575     


Ile Pro Asp Arg Phe Ser Gly Ser Arg Trp Gly Pro Asp Tyr Asn Leu 
            580                 585                 590         


Thr Ile Ser Asn Leu Glu Ser Gly Asp Phe Gly Val Tyr Tyr Cys Gln 
        595                 600                 605             


Gln Tyr Glu Phe Phe Gly Gln Gly Thr Lys Val Gln Val Asp Ile Lys 
    610                 615                 620                 


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
625                 630                 635                 640 


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
                645                 650                 655     


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
            660                 665                 670         


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
        675                 680                 685             


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
    690                 695                 700                 


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Arg Ser 
705                 710                 715                 720 


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
                725                 730     


<210>  6
<211>  819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized Nucleic Acid Sequence Encoding IgG Heavy Chain

<400>  6
ggatccgcca ccatggaaac cgacactctg ctgctgtggg tgctgctgct gtgggtgccc       60

ggctcaacag gcgacggcgc tcaggtccag ctggtccagt ctggagctgt gatcaagacc      120

cctggcagct ccgtcaaaat ttcttgcaga gcaagtggct acaacttccg ggactatagc      180

atccactggg tgcggctgat tcctgataag ggatttgagt ggatcggctg gatcaagcca      240

ctgtggggcg ctgtgtccta cgcaaggcag ctgcaggggc gcgtctccat gacacgacag      300

ctgtctcagg acccagacga tcccgattgg ggggtggcct acatggagtt cagtggactg      360

actcccgcag acaccgccga atatttttgc gtgcggagag gctcctgcga ctactgtggg      420

gatttcccat ggcagtattg gtgtcaggga actgtggtcg tggtctctag tgcatcaacc      480

aagggcccca gcgtgtttcc tctggcccca tcaagcaaaa gtacatcagg aggaactgca      540

gctctgggat gtctggtgaa ggattacttc cccgagcctg tgaccgtcag ctggaactcc      600

ggagcactga cctccggagt gcacacattt cccgctgtcc tgcagtcctc tgggctgtac      660

tctctgagtt cagtggtcac agtgcctagc tcctctctgg gcacccagac atatatctgc      720

aacgtcaatc ataagccaag taatactaaa gtggacaaga aagtcgaacc caaatcatgt      780

tacccctatg acgtgcctga ttatgcttga taactcgag                             819


<210>  7
<211>  753
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized Nucleic Acid Sequence Encoding IgG Light Chain

<400>  7
ggatccgcca ccatggagac tgatacactg ctgctgtggg tgctgctgct gtgggtgcct       60

ggctcaaccg gcgacggggc tcaggtccag attgtgctga cccagagccc tggcatcctg      120

tcactgagcc caggagagac cgcaacactg ttctgcaagg cctcccaggg cgggaacgct      180

atgacatggt accagaaacg gagaggacag gtgccccgac tgctgatcta tgacacttca      240

aggcgagcaa gcggagtgcc tgatcgattt gtcggcagcg gctctgggac agacttcttt      300

ctgactatta ataagctgga cagagaggat ttcgctgtgt actattgcca gcagtttgaa      360

ttctttggac tgggcagcga gctggaagtg cacaggaccg tcgccgctcc aagtgtgttc      420

atttttcccc ctagcgatga gcagctgaaa tccgggacag cctctgtggt ctgtctgctg      480

aacaatttct acccccgcga agcaaaggtg cagtggaaag tcgacaacgc cctgcagagt      540

ggcaattcac aggagagcgt gaccgaacag gactccaagg attctacata tagtctgagc      600

tccactctga ccctgtctaa agctgattac gagaagcaca aagtgtatgc atgcgaagtc      660

actcatcagg gcctgtctag tcctgtgacc aagagcttta accgagggga gtgttaccca      720

tatgacgtcc ccgattacgc ctgataactc gag                                   753


<210>  8
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgE1 Signal Peptide of VRC-1 IgG

<400>  8

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


<210>  9
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable Heavy Region of VRC01 IgG

<400>  9

Gln Val Gln Leu Val Gln Ser Gly Gly Gln Met Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Met Arg Ile Ser Cys Arg Ala Ser Gly Tyr Glu Phe Ile Asp Cys 
            20                  25                  30          


Thr Leu Asn Trp Ile Arg Leu Ala Pro Gly Lys Arg Pro Glu Trp Met 
        35                  40                  45              


Gly Trp Leu Lys Pro Arg Gly Gly Ala Val Asn Tyr Ala Arg Pro Leu 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Val Tyr Ser Asp Thr Ala Phe 
65                  70                  75                  80  


Leu Glu Leu Arg Ser Leu Thr Val Asp Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Gly Lys Asn Cys Asp Tyr Asn Trp Asp Phe Glu His Trp Gly 
            100                 105                 110         


Arg Gly Thr Pro Val Ile Val Ser Ser Pro Ser Thr Lys Gly 
        115                 120                 125     


<210>  10
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy region 1 (CH1) of VRC01 IgG

<400>  10

Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
1               5                   10                  15      


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
            20                  25                  30          


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
        35                  40                  45              


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
    50                  55                  60                  


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
65                  70                  75                  80  


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Ala Glu Pro Lys 
                85                  90                  95      


Ser Cys 
        


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Region of VRC01 IgG

<400>  11

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  12
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy Region 2 (CH2) of VRC01 IgG

<400>  12

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  13
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy Region 3 (CH3) of VRC01 IgG

<400>  13

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
1               5                   10                  15      


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            100                 105         


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin Cleavage Site of VRC01 IgG

<400>  14

Arg Gly Arg Lys Arg Arg Ser 
1               5           


<210>  15
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSG Linker and P2A Peptide of VRC01 IgG

<400>  15

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  16
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable Light Region (VL) of VRC01 IgG

<400>  16

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Thr Ala Ile Ile Ser Cys Arg Thr Ser Gln Tyr Gly Ser Leu Ala 
            20                  25                  30          


Trp Tyr Gln Gln Arg Pro Gly Gln Ala Pro Arg Leu Val Ile Tyr Ser 
        35                  40                  45              


Gly Ser Thr Arg Ala Ala Gly Ile Pro Asp Arg Phe Ser Gly Ser Arg 
    50                  55                  60                  


Trp Gly Pro Asp Tyr Asn Leu Thr Ile Ser Asn Leu Glu Ser Gly Asp 
65                  70                  75                  80  


Phe Gly Val Tyr Tyr Cys Gln Gln Tyr Glu Phe Phe Gly Gln Gly Thr 
                85                  90                  95      


Lys Val Gln Val Asp Ile Lys Arg 
            100                 


<210>  17
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Light Region (CL, kappa) of VRC01 IgG

<400>  17

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Arg Ser Pro 
                85                  90                  95      


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105     


<210>  18
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG Heavy Chain Signal Peptide of HIV-1 Env-PG9 Ig

<400>  18

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala 
            


<210>  19
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable Heavy Region of HIV-1 Env-PG9 Ig

<400>  19

Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Phe Leu Arg Gly Val 
1               5                   10                  15      


Gln Cys Gln Arg Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly 
            20                  25                  30          


Ser Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg 
        35                  40                  45              


Gln Gly Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp 
    50                  55                  60                  


Val Ala Phe Ile Lys Tyr Asp Gly Ser Glu Lys Tyr His Ala Asp Ser 
65                  70                  75                  80  


Val Trp Gly Arg Leu Ser Ile Ser Arg Asp Asn Ser Lys Asp Thr Leu 
                85                  90                  95      


Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Thr Tyr Phe 
            100                 105                 110         


Cys Val Arg Glu Ala Gly Gly Pro Asp Tyr Arg Asn Gly Tyr Asn Tyr 
        115                 120                 125             


Tyr Asp Phe Tyr Asp Gly Tyr Tyr Asn Tyr His Tyr Met Asp Val Trp 
    130                 135                 140                 


Gly Lys Gly Thr Thr Val Thr Val Ser Ser 
145                 150                 


<210>  20
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy region 1 (CH1) of HIV-1 Env-PG9 Ig

<400>  20

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val 
        


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Region of HIV-1 Env-PG9 Ig

<400>  21

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  22
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy Region 2 (CH2) of HIV-1 Env-PG9 Ig

<400>  22

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  23
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy Region 3 (CH3) of HIV-1 Env-PG9 Ig

<400>  23

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
1               5                   10                  15      


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            100                 105         


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin Cleavage Site of HIV-1 Env-PG9 Ig

<400>  24

Arg Gly Arg Lys Arg Arg Ser 
1               5           


<210>  25
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSG Linker and P2A Peptide of HIV-1 Env-PG9 Ig

<400>  25

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Lamba Light Chain Signal Peptide of HIV-1 Env-PG9 Ig

<400>  26

Met Ala Trp Thr Pro Leu Phe Leu Phe Leu Leu Thr Cys Cys Pro Gly 
1               5                   10                  15      


Gly Ser Asn Ser 
            20  


<210>  27
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable Light Region (VL) of HIV-1 Env-PG9 Ig

<400>  27

Gln Ser Ala Leu Thr Gln Pro Ala Ser Val Ser Gly Ser Pro Gly Gln 
1               5                   10                  15      


Ser Ile Thr Ile Ser Cys Asn Gly Thr Ser Asn Asp Val Gly Gly Tyr 
            20                  25                  30          


Glu Ser Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Val 
        35                  40                  45              


Val Ile Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Ser Asn Arg Phe 
    50                  55                  60                  


Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Gly Asp Tyr Tyr Cys Lys Ser Leu Thr Ser Thr 
                85                  90                  95      


Arg Arg Arg Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110 


<210>  28
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Light Region (CL, lamba) of HIV-1 Env-PG9 Ig

<400>  28

Gly Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser 
1               5                   10                  15      


Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp 
            20                  25                  30          


Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro 
        35                  40                  45              


Val Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn 
    50                  55                  60                  


Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys 
65                  70                  75                  80  


Ser His Lys Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val 
                85                  90                  95      


Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
            100                 105     


<210>  29
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG Heavy Chain Signal Peptide of HIV-1 Env-4E10 Ig

<400>  29

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala 
            


<210>  30
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable Heavy Region of HIV-1 Env-4E10 Ig

<400>  30

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Thr Val Ser Cys Lys Ala Ser Gly Gly Ser Phe Ser Thr Tyr 
            20                  25                  30          


Ala Leu Ser Trp Val Arg Gln Ala Pro Gly Arg Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Val Ile Pro Leu Leu Thr Ile Thr Asn Tyr Ala Pro Arg Phe 
    50                  55                  60                  


Gln Gly Arg Ile Thr Ile Thr Ala Asp Arg Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Gly Thr Thr Gly Trp Gly Trp Leu Gly Lys Pro Ile Gly 
            100                 105                 110         


Ala Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125         


<210>  31
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy region 1 (CH1) of HIV-1 Env-4E10 Ig

<400>  31

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val 
        


<210>  32
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Region of HIV-1 Env-4E10 Ig

<400>  32

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  33
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy Region 2 (CH2) of HIV-1 Env-4E10 Ig

<400>  33

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  34
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Heavy Region 3 (CH3) of HIV-1 Env-4E10 Ig

<400>  34

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
1               5                   10                  15      


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            100                 105         


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin Cleavage Site of HIV-1 Env-4E10 Ig

<400>  35

Arg Gly Arg Lys Arg Arg Ser 
1               5           


<210>  36
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSG Linker and P2A Peptide of HIV-1 Env-4E10 Ig

<400>  36

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  37
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Kappa Light Chain Signal Peptide of HIV-1 Env-4E10 Ig

<400>  37

Met Val Leu Gln Thr Gln Val Phe Ile Ser Leu Leu Leu Trp Ile Ser 
1               5                   10                  15      


Gly Ala Tyr Gly 
            20  


<210>  38
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable Light Region (VL) of HIV-1 Env-4E10 Ig

<400>  38

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Gln Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Asn Asn 
            20                  25                  30          


Lys Leu Ala Trp Tyr Gln Gln Arg Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Pro Ser Gly Val Ala Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Gln Ser Leu 
                85                  90                  95      


Ser Thr Phe Gly Gln Gly Thr Lys Val Glu 
            100                 105     


<210>  39
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant Light Region (CL, kappa) of HIV-1 Env-4E10 Ig

<400>  39

Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
1               5                   10                  15      


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
            20                  25                  30          


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
        35                  40                  45              


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
    50                  55                  60                  


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
65                  70                  75                  80  


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
                85                  90                  95      


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu 
            100                 105         


<210>  40
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding the VH-CH1 of anti-Her-2 Fab

<400>  40
ggatccgcca ccatggactg gacatggatt ctgtttctgg tcgccgccgc tacaagagtg       60

cattccgaag tgcagctggt cgagagtgga gggggactgg tgcagcccgg cggatctctg      120

cgactgagtt gcgccgcttc aggcttcacc tttacagact acaccatgga ttgggtgaga      180

caggcacctg gcaagggact ggagtgggtg gctgatgtca acccaaatag tgggggctca      240

atctacaacc agaggttcaa gggcaggttc accctgagcg tggacaggtc caaaaacact      300

ctgtatctgc agatgaattc tctgcgggct gaagataccg cagtctacta ttgcgcccgc      360

aatctgggcc caagcttcta ctttgactat tgggggcagg gcacactggt gactgtcagc      420

tccgcttcta caaagggacc aagcgtgttc ccactggcac cctctagtaa atccacctct      480

ggagggacag cagccctggg ctgtctggtg aaagactatt tccccgagcc tgtgactgtc      540

agctggaact ccggagcact gactagcgga gtgcacacct ttccagccgt cctgcagtca      600

agcggcctgt actccctgtc ctctgtggtc acagtgccta gttcaagcct gggaactcag      660

acctatattt gtaatgtgaa ccataaacca agcaatacaa aggtggacaa gaaggtggaa      720

ccaaaatcct gctgataact cgag                                             744


<210>  41
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the VH-CH1 of anti-Her-2 Fab

<400>  41

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
            20                  25                  30          


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr 
        35                  40                  45              


Asp Tyr Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Val Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln 
65                  70                  75                  80  


Arg Phe Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


<210>  42
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding the VL-CL of anti-Her-2 Fab

<400>  42
ggatccgcca ccatggattg gacttggatt ctgttcctgg tcgccgccgc tacccgcgtg       60

cattccgata ttcagatgac tcagagcccc tcctcactgt cagccagcgt gggcgaccga      120

gtcaccatca catgcaaagc ttctcaggat gtgagtattg gggtcgcatg gtaccagcag      180

aagccaggca aagcacccaa gctgctgatc tattccgcct cttacaggta tacaggagtg      240

cccagcagat tcagtggctc aggaagcggg actgacttta ctctgaccat cagctccctg      300

cagcctgagg atttcgctac ctactattgc cagcagtact atatctaccc atataccttt      360

ggccagggaa caaaagtgga gatcaagcgg accgtggccg ctccctccgt cttcattttt      420

cccccttctg acgaacagct gaagagcgga acagcaagcg tggtctgtct gctgaacaat      480

ttctaccctc gcgaggccaa agtgcagtgg aaggtcgata acgctctgca gtccgggaat      540

tctcaggaga gtgtgactga acaggactca aaagatagca cctattccct gtctagtaca      600

ctgactctga gcaaggcaga ctacgaaaag cacaaagtgt atgcctgtga ggtcacccac      660

caggggctgt caagtcccgt caccaagtcc ttcaatagag gcgaatgctg ataactcgag      720


<210>  43
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the VL-CL of anti-Her-2 Fab

<400>  43

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
            20                  25                  30          


Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser 
        35                  40                  45              


Ile Gly Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
    50                  55                  60                  


Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
                85                  90                  95      


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr 
            100                 105                 110         


Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val 
        115                 120                 125             


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
    130                 135                 140                 


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
145                 150                 155                 160 


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
                165                 170                 175     


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
            180                 185                 190         


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
        195                 200                 205             


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
    210                 215                 220                 


Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230         


<210>  44
<211>  2241
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding anti-DENV Human IgG

<400>  44
ggatccgcca ccatggactg gacttggagg attctgtttc tggtcgccgc cgctactggg       60

actcacgctc aggcacatct ggtcgaatct ggaggaggag tggtccagcc tggccgatcc      120

ctgcgactgt cttgcgcagc tagcgccttc aacttcagca caaacgcaat gcactgggtg      180

cgacaggcac caggcaaggg actggagtgg gtcgctgtga tctcatacga cggaagccat      240

aagtactatg cagattctgt gaaaggccgg ttcaccattt ccagggacaa ttctaagaac      300

accctgtatc tgcagatgaa tagcctgcgc gcagccgata ccgcagtgta ctattgcgca      360

actgtcggcg tgctgacctg gccagtgaac gccgaatact ttcaccattg gggacagggc      420

agtctggtct cagtgagctc cgcaagtact aagggaccat cagtgttccc actggcaccc      480

tctagtaaat ctactagtgg cgggaccgct gcactgggat gtctggtgaa ggactatttc      540

cccgagcctg tcaccgtgag ctggaattcc ggagccctga caagcggcgt ccacactttt      600

cccgctgtgc tgcagtcaag cggactgtac tccctgtcct ctgtggtcac tgtgcctagt      660

tcaagcctgg gcactcagac ctatatctgc aatgtgaacc acaagccctc taacaccaaa      720

gtcgacaaga aagtggaacc taagagctgt gataaaacac atacttgccc accttgtcca      780

gcaccagagc tgctgggagg accaagcgtg ttcctgtttc cacccaagcc taaagacaca      840

ctgatgatta gccggacacc tgaagtcact tgcgtggtcg tggacgtgtc ccacgaggac      900

cccgaagtca agtttaattg gtacgtggat ggcgtcgagg tgcataacgc caagaccaaa      960

ccccgggagg aacagtacaa tagcacatat agagtcgtgt ccgtcctgac tgtgctgcat     1020

caggattggc tgaatgggaa ggagtataag tgcaaagtgt ctaacaaggc tctgcctgca     1080

ccaatcgaga aaaccattag caaggctaaa ggccagccta gggaaccaca ggtgtacaca     1140

ctgcctccaa gtcgcgacga gctgaccaag aatcaggtct ccctgacatg tctggtgaaa     1200

ggcttctatc catcagatat cgccgtggag tgggaaagca acgggcagcc cgaaaacaat     1260

tacaagacca caccccctgt gctggactct gatggcagtt tctttctgta ttctaagctg     1320

accgtggaca aaagtagatg gcagcagggg aatgtctttt catgtagcgt gatgcacgag     1380

gccctgcaca accattacac acagaagtcc ctgtctctga gtcccggaaa gaggggccgc     1440

aaacggagat cagggagcgg agctactaat ttcagcctgc tgaaacaggc aggggatgtg     1500

gaggaaaacc ccggacctat ggcttggacc ccactgttcc tgtttctgct gacatgctgt     1560

cccgggggca gcaattctca gagtgtcctg acacagccac catcagtgag cggagcacca     1620

ggacagaggg tgaccatctc ctgcacaggc agcagcagca acattggcgc cgggtacgac     1680

gtgcattggt atcagcagct gcccggcacc gctcctaagc tgctgatctg tggcaacaat     1740

aaccgcccat ctggggtgcc cgatcgattc tccggctcta aaagtgggac ttcagccagc     1800

ctggctatta ccggcctgca ggccgaggac gaagctgatt actattgcca gagctacgac     1860

tcaagcctga ccggagtcgt gttcggagga ggaaccaagc tgacagtcct gggacagcct     1920

aaagccgctc caagcgtgac actgtttcct ccatcctctg aggaactgca ggcaaacaag     1980

gccaccctgg tgtgcctgat ttccgacttc taccccgggg cagtcactgt ggcttggaag     2040

gcagatagtt cacctgtcaa agccggagtg gagactacca caccatcaaa gcagagcaat     2100

aacaaatacg cagccagctc ctatctgtcc ctgacccctg agcagtggaa gtctcacaaa     2160

tcctattctt gccaggtcac tcacgaagga agcactgtgg agaaaactgt cgcaccaacc     2220

gaatgtagtt gataactcga g                                               2241


<210>  45
<211>  739
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of anti-DENV Human IgG

<400>  45

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Gln Ala His Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Ala Phe Asn Phe 
        35                  40                  45              


Ser Thr Asn Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser His Lys Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Val Gly Val Leu Thr Trp Pro Val Asn Ala Glu 
        115                 120                 125             


Tyr Phe His His Trp Gly Gln Gly Ser Leu Val Ser Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser 
465                 470                 475                 480 


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
                485                 490                 495     


Glu Glu Asn Pro Gly Pro Met Ala Trp Thr Pro Leu Phe Leu Phe Leu 
            500                 505                 510         


Leu Thr Cys Cys Pro Gly Gly Ser Asn Ser Gln Ser Val Leu Thr Gln 
        515                 520                 525             


Pro Pro Ser Val Ser Gly Ala Pro Gly Gln Arg Val Thr Ile Ser Cys 
    530                 535                 540                 


Thr Gly Ser Ser Ser Asn Ile Gly Ala Gly Tyr Asp Val His Trp Tyr 
545                 550                 555                 560 


Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Cys Gly Asn Asn 
                565                 570                 575     


Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly 
            580                 585                 590         


Thr Ser Ala Ser Leu Ala Ile Thr Gly Leu Gln Ala Glu Asp Glu Ala 
        595                 600                 605             


Asp Tyr Tyr Cys Gln Ser Tyr Asp Ser Ser Leu Thr Gly Val Val Phe 
    610                 615                 620                 


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro 
625                 630                 635                 640 


Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys 
                645                 650                 655     


Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr 
            660                 665                 670         


Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr 
        675                 680                 685             


Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr 
    690                 695                 700                 


Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys 
705                 710                 715                 720 


Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr 
                725                 730                 735     


Glu Cys Ser 
            


<210>  46
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG Heavy Chain

<400>  46

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Gly Ala Gln Val Gln Leu Val Gln Ser Gly Ala 
            20                  25                  30          


Val Ile Lys Thr Pro Gly Ser Ser Val Lys Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gly Tyr Asn Phe Arg Asp Tyr Ser Ile His Trp Val Arg Leu Ile Pro 
    50                  55                  60                  


Asp Lys Gly Phe Glu Trp Ile Gly Trp Ile Lys Pro Leu Trp Gly Ala 
65                  70                  75                  80  


Val Ser Tyr Ala Arg Gln Leu Gln Gly Arg Val Ser Met Thr Arg Gln 
                85                  90                  95      


Leu Ser Gln Asp Pro Asp Asp Pro Asp Trp Gly Val Ala Tyr Met Glu 
            100                 105                 110         


Phe Ser Gly Leu Thr Pro Ala Asp Thr Ala Glu Tyr Phe Cys Val Arg 
        115                 120                 125             


Arg Gly Ser Cys Asp Tyr Cys Gly Asp Phe Pro Trp Gln Tyr Trp Cys 
    130                 135                 140                 


Gln Gly Thr Val Val Val Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
145                 150                 155                 160 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
                165                 170                 175     


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
            180                 185                 190         


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
        195                 200                 205             


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
    210                 215                 220                 


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
225                 230                 235                 240 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
                245                 250                 255     


Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
            260                 265 


<210>  47
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG Light Chain

<400>  47

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Gly Ala Gln Val Gln Ile Val Leu Thr Gln Ser 
            20                  25                  30          


Pro Gly Ile Leu Ser Leu Ser Pro Gly Glu Thr Ala Thr Leu Phe Cys 
        35                  40                  45              


Lys Ala Ser Gln Gly Gly Asn Ala Met Thr Trp Tyr Gln Lys Arg Arg 
    50                  55                  60                  


Gly Gln Val Pro Arg Leu Leu Ile Tyr Asp Thr Ser Arg Arg Ala Ser 
65                  70                  75                  80  


Gly Val Pro Asp Arg Phe Val Gly Ser Gly Ser Gly Thr Asp Phe Phe 
                85                  90                  95      


Leu Thr Ile Asn Lys Leu Asp Arg Glu Asp Phe Ala Val Tyr Tyr Cys 
            100                 105                 110         


Gln Gln Phe Glu Phe Phe Gly Leu Gly Ser Glu Leu Glu Val His Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Tyr Pro Tyr Asp Val Pro 
225                 230                 235                 240 


Asp Tyr Ala 
            


<210>  48
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the Heavy Chain (VH-CH1) of HIV-1 Env Fab

<400>  48

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Gly Ala Gln Val Gln Leu Val Gln Ser Gly Gly 
            20                  25                  30          


Gln Met Lys Lys Pro Gly Glu Ser Met Arg Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gly Tyr Glu Phe Ile Asp Cys Thr Leu Asn Trp Ile Arg Leu Ala Pro 
    50                  55                  60                  


Gly Lys Arg Pro Glu Trp Met Gly Trp Leu Lys Pro Arg Gly Gly Ala 
65                  70                  75                  80  


Val Asn Tyr Ala Arg Pro Leu Gln Gly Arg Val Thr Met Thr Arg Asp 
                85                  90                  95      


Val Tyr Ser Asp Thr Ala Phe Leu Glu Leu Arg Ser Leu Thr Val Asp 
            100                 105                 110         


Asp Thr Ala Val Tyr Phe Cys Thr Arg Gly Lys Asn Cys Asp Tyr Asn 
        115                 120                 125             


Trp Asp Phe Glu His Trp Gly Arg Gly Thr Pro Val Ile Val Ser Ser 
    130                 135                 140                 


Pro Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
145                 150                 155                 160 


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
                165                 170                 175     


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
            180                 185                 190         


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
        195                 200                 205             


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
    210                 215                 220                 


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
225                 230                 235                 240 


Lys Ala Glu Pro Lys Ser Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
                245                 250                 255     


<210>  49
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the Light Chain (VL-CL) of HIV-1 Env Fab

<400>  49

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Gly Ala Gln Val Gln Ile Val Leu Thr Gln Ser 
            20                  25                  30          


Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Thr Ala Ile Ile Ser Cys 
        35                  40                  45              


Arg Thr Ser Gln Tyr Gly Ser Leu Ala Trp Tyr Gln Gln Arg Pro Gly 
    50                  55                  60                  


Gln Ala Pro Arg Leu Val Ile Tyr Ser Gly Ser Thr Arg Ala Ala Gly 
65                  70                  75                  80  


Ile Pro Asp Arg Phe Ser Gly Ser Arg Trp Gly Pro Asp Tyr Asn Leu 
                85                  90                  95      


Thr Ile Ser Asn Leu Glu Ser Gly Asp Phe Gly Val Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Tyr Glu Phe Phe Gly Gln Gly Thr Lys Val Gln Val Asp Ile Lys 
        115                 120                 125             


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
    130                 135                 140                 


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
145                 150                 155                 160 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
                165                 170                 175     


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
            180                 185                 190         


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
        195                 200                 205             


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Arg Ser 
    210                 215                 220                 


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Tyr Pro Tyr Asp Val 
225                 230                 235                 240 


Pro Asp Tyr Ala 
                


<210>  50
<211>  1536
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding HIV-1 PG9 Fab

<400>  50
ggatccgcca ccatggcaag acccctgtgc accctgctgc tgctgatggc aaccctggcc       60

ggagccctgg cacagagcgc cctgacccag cccgcaagcg tctccggctc accaggccag      120

agcatcacta ttagttgcaa cgggactagc aacgacgtgg gaggctatga gagtgtcagc      180

tggtaccagc agcatcccgg aaaagcacca aaagtggtca tctacgatgt cagtaaaagg      240

ccaagtgggg tctcaaatag gttctcaggg agtaaatctg ggaatacagc atctctgacc      300

atctccggac tgggcgcaga agatgaaggc gactactatt gcaaaagcct gacctcaacc      360

agacggcgag tctttgggac aggcaccaag ctgacagtcc tgacagtcgc tgccccctcc      420

gtcttcattt ttccaccttc agatgagcag ctgaaatctg gcactgcatc tgtggtctgc      480

ctgctgaaca acttctatcc acgagaggcc aaggtgcagt ggaaagtgga taacgcactg      540

cagtccggca atagtcagga aagcgtgact gagcaggatt ccaaggacag tacctatagc      600

ctgtccagta cactgaccct gtccaaggct gactacgaaa aacataaggt gtatgcatgt      660

gaagtgactc accagggact gaggtcacca gtcactaagt cttttaacag gggagagtgc      720

ggcgggggag gatctggagg cggcggctct ggagggggag gctcaggggg cggaggaagc      780

ggcggaggag ggtccggagg aggaggcagt cagagactgg tcgaaagcgg gggaggagtg      840

gtgcagcctg ggtcctcact gagactgtca tgcgctgcca gtggctttga tttttcacga      900

cagggaatgc attgggtcag gcaggcaccc ggacagggcc tggaatgggt cgccttcatt      960

aagtacgacg gaagcgagaa gtaccatgcc gactcagtgt ggggaaggct gagcatctca     1020

agggacaact caaaggacac cctgtacctg cagatgaata gcctgagagt ggaagatacc     1080

gctacttatt tctgcgtgcg agaggccgga gggccagatt accggaacgg gtacaattac     1140

tatgatttct acgacggcta ctacaattac cattatatgg atgtctgggg caaaggaact     1200

acagtcaccg tgagctccgc aagtactaag ggaccttccg tgtttcctct ggctcccagt     1260

tccaaaagta catccggagg aacagccgct ctgggatgtc tggtcaagga ctattttccc     1320

gagcccgtga ctgtctcctg gaacagcggg gctctgacaa gcggggtgca cacctttcct     1380

gccgtgctgc agtccagtgg gctgtacagt ctgtctagtg tcgtcactgt gccaagctca     1440

agtctgggga cccagacata catttgtaat gtgaaccata aaccctcaaa caccaaagtg     1500

gacaagaaag tggaacctaa aagctgataa ctcgag                               1536


<210>  51
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of HIV-1 PG9 Fab

<400>  51

Met Ala Arg Pro Leu Cys Thr Leu Leu Leu Leu Met Ala Thr Leu Ala 
1               5                   10                  15      


Gly Ala Leu Ala Gln Ser Ala Leu Thr Gln Pro Ala Ser Val Ser Gly 
            20                  25                  30          


Ser Pro Gly Gln Ser Ile Thr Ile Ser Cys Asn Gly Thr Ser Asn Asp 
        35                  40                  45              


Val Gly Gly Tyr Glu Ser Val Ser Trp Tyr Gln Gln His Pro Gly Lys 
    50                  55                  60                  


Ala Pro Lys Val Val Ile Tyr Asp Val Ser Lys Arg Pro Ser Gly Val 
65                  70                  75                  80  


Ser Asn Arg Phe Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr 
                85                  90                  95      


Ile Ser Gly Leu Gly Ala Glu Asp Glu Gly Asp Tyr Tyr Cys Lys Ser 
            100                 105                 110         


Leu Thr Ser Thr Arg Arg Arg Val Phe Gly Thr Gly Thr Lys Leu Thr 
        115                 120                 125             


Val Leu Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
    130                 135                 140                 


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
145                 150                 155                 160 


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
                165                 170                 175     


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
            180                 185                 190         


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
        195                 200                 205             


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Arg 
    210                 215                 220                 


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Gly Gly 
225                 230                 235                 240 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                245                 250                 255     


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Arg Leu Val Glu Ser 
            260                 265                 270         


Gly Gly Gly Val Val Gln Pro Gly Ser Ser Leu Arg Leu Ser Cys Ala 
        275                 280                 285             


Ala Ser Gly Phe Asp Phe Ser Arg Gln Gly Met His Trp Val Arg Gln 
    290                 295                 300                 


Ala Pro Gly Gln Gly Leu Glu Trp Val Ala Phe Ile Lys Tyr Asp Gly 
305                 310                 315                 320 


Ser Glu Lys Tyr His Ala Asp Ser Val Trp Gly Arg Leu Ser Ile Ser 
                325                 330                 335     


Arg Asp Asn Ser Lys Asp Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg 
            340                 345                 350         


Val Glu Asp Thr Ala Thr Tyr Phe Cys Val Arg Glu Ala Gly Gly Pro 
        355                 360                 365             


Asp Tyr Arg Asn Gly Tyr Asn Tyr Tyr Asp Phe Tyr Asp Gly Tyr Tyr 
    370                 375                 380                 


Asn Tyr His Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Thr Val 
385                 390                 395                 400 


Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
                405                 410                 415     


Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys 
            420                 425                 430         


Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
        435                 440                 445             


Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
    450                 455                 460                 


Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
465                 470                 475                 480 


Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
                485                 490                 495     


Asp Lys Lys Val Glu Pro Lys Ser 
            500                 


<210>  52
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding HIV-1 4E10 Fab

<400>  52
ggatccgcca ccatggcaag acctctgtgc actctgctgc tgctgatggc tactctggcc       60

ggggctctgg ctgagattgt cctgacccag tcccctggca ctcagtcact gtcccccggc      120

gagcgcgcaa ctctgtcctg cagagcaagc cagtccgtcg ggaacaacaa gctggcatgg      180

taccagcagc gcccaggaca ggcacccagg ctgctgatct acggagcaag ctcccggcct      240

agcggagtcg ctgatagatt ctccggaagc ggctccggga ccgatttcac tctgaccatc      300

tccaggctgg aacctgagga ttttgccgtg tattactgtc agcagtacgg gcagagcctg      360

tcaactttcg gccagggaac taaagtcgaa aagagaaccg tggccgcacc aagcgtcttt      420

atttttcccc ctagcgatga acagctgaaa tccgggactg cttccgtggt ctgcctgctg      480

aataacttct atccaagaga ggcaaaggtg cagtggaaag tggacaacgc cctgcagagc      540

ggaaactcac aggaatctgt gacagagcag gactccaagg atagcacata cagtctgtcc      600

tcaactctga ccctgtccaa agctgactat gagaagcata aagtctacgc atgtgaggtg      660

acccaccagg gactgaggtc ccccgtcact aagtccttca atagaggcga gtgcgggggc      720

gggggcagtg gcggaggggg aagtgggggc ggagggagtg gcggcggcgg gagtggcggc      780

ggcggctcag ggggcggcgg ctcccaggtc cagctggtcc agagcggagc cgaggtcaag      840

agaccaggct cttcagtcac cgtgagctgc aaagccagcg gaggctcctt tagcacttac      900

gccctgtcat gggtgcggca ggccccaggc cgaggcctgg agtggatggg cggcgtgatc      960

cccctgctga ccattactaa ctatgcccct agatttggag gccggatcac catcacagct     1020

gacagatcca catccacagc ttacctggag ctgaacagtc tgaggcccga ggacactgca     1080

gtctactact gtgcacgaga aggcaccact ggatgggggt ggctggggaa gcccatcggg     1140

gcttttgcac attggggcgg agggacactg gtgactgtga gctctgccag cactaaaggg     1200

cccagtgtct tccctctggc cccaagttcc aagagtacat cagggggcac cgccgcactg     1260

gggtgtctgg tgaaggatta cttcccagag cccgtgacag tcagttggaa cagcggcgct     1320

ctgaccagtg gggtgcacac tttcccagcc gtgctgcaga gttcagggct gtactccctg     1380

tcctcagtgg tgactgtgcc ctcaagcagt ctggggactc agacttacat ttgtaatgtg     1440

aaccataaac cctcaaatac taaagtggac aaaaaagtgg aaccaaagag ctgataactc     1500

gag                                                                   1503


<210>  53
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of HIV-1 4E10 Fab

<400>  53

Met Ala Arg Pro Leu Cys Thr Leu Leu Leu Leu Met Ala Thr Leu Ala 
1               5                   10                  15      


Gly Ala Leu Ala Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Gln Ser 
            20                  25                  30          


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser 
        35                  40                  45              


Val Gly Asn Asn Lys Leu Ala Trp Tyr Gln Gln Arg Pro Gly Gln Ala 
    50                  55                  60                  


Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Pro Ser Gly Val Ala 
65                  70                  75                  80  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                85                  90                  95      


Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Gly Gln Ser Leu Ser Thr Phe Gly Gln Gly Thr Lys Val Glu Lys Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Arg Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Gly Gly Ser Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly 
            260                 265                 270         


Ala Glu Val Lys Arg Pro Gly Ser Ser Val Thr Val Ser Cys Lys Ala 
        275                 280                 285             


Ser Gly Gly Ser Phe Ser Thr Tyr Ala Leu Ser Trp Val Arg Gln Ala 
    290                 295                 300                 


Pro Gly Arg Gly Leu Glu Trp Met Gly Gly Val Ile Pro Leu Leu Thr 
305                 310                 315                 320 


Ile Thr Asn Tyr Ala Pro Arg Phe Gly Gly Arg Ile Thr Ile Thr Ala 
                325                 330                 335     


Asp Arg Ser Thr Ser Thr Ala Tyr Leu Glu Leu Asn Ser Leu Arg Pro 
            340                 345                 350         


Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Gly Thr Thr Gly Trp 
        355                 360                 365             


Gly Trp Leu Gly Lys Pro Ile Gly Ala Phe Ala His Trp Gly Gly Gly 
    370                 375                 380                 


Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
385                 390                 395                 400 


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
                405                 410                 415     


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
            420                 425                 430         


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
        435                 440                 445             


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
    450                 455                 460                 


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
465                 470                 475                 480 


Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser 
                485                 490             


<210>  54
<211>  1446
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding the HIV-1 VRC01 IgG1 Heavy Chain 
       (VH/CH1/Hinge/CH2/CH3)

<400>  54
ggatccgcca ccatggattg gacatggatt ctgttcctgg tcgccgccgc aactagagtg       60

cattcacagg tgcagctggt gcagtcaggc gggcagatga agaaacccgg cgagagtatg      120

cgaatctcat gccgggctag cgggtacgaa ttcatcgact gtaccctgaa ctggattaga      180

ctggcacctg ggaagaggcc agagtggatg ggatggctga aacctagagg cggggcagtg      240

aattacgcca gaccactgca gggcagggtc actatgaccc gcgacgtgta ttctgatacc      300

gcattcctgg agctgcgaag tctgacagtc gacgatactg ccgtgtactt ctgcacacgg      360

ggcaagaact gtgactataa ttgggatttt gaacactggg gcagggggac acctgtcatt      420

gtgagctccc caagtactaa gggaccctca gtgtttcccc tggccccttc tagtaaaagt      480

acctcaggag gcacagccgc tctgggatgc ctggtgaagg attacttccc tgagccagtc      540

accgtgagtt ggaactcagg cgccctgaca agcggggtcc atacttttcc agctgtgctg      600

cagtcaagcg ggctgtactc cctgtcctct gtggtcacag tgcccagttc aagcctggga      660

acacagactt atatctgtaa cgtcaatcac aagcctagca atactaaagt ggacaagaaa      720

gccgagccta agagctgcga accaaagtcc tgtgataaaa cccatacatg ccctccctgt      780

ccagctcctg aactgctggg cggcccatcc gtgttcctgt ttccacccaa gcccaaagac      840

accctgatga ttagcaggac tcctgaggtc acctgcgtgg tcgtggacgt gtcccacgag      900

gaccccgaag tcaagtttaa ctggtacgtg gatggcgtcg aagtgcataa tgccaagaca      960

aaaccccggg aggaacagta caactctacc tatagagtcg tgagtgtcct gacagtgctg     1020

caccaggact ggctgaacgg gaaggagtat aagtgcaaag tgtctaataa ggccctgcca     1080

gctcccatcg agaaaacaat ttccaaggca aaaggccagc caagggaacc ccaggtgtac     1140

actctgcctc catcccgcga cgagctgact aagaaccagg tctctctgac ctgtctggtg     1200

aaaggattct atccaagcga tatcgccgtg gagtgggaat ccaatggcca gcccgagaac     1260

aattacaaga ccacaccccc tgtgctggac agcgatggct ccttctttct gtattcaaag     1320

ctgaccgtgg ataaaagccg ctggcagcag gggaacgtct ttagctgctc cgtgatgcac     1380

gaagctctgc acaatcatta cacccagaag tctctgagtc tgtcacctgg caagtgataa     1440

ctcgag                                                                1446


<210>  55
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the HIV-1 VRC01 IgG1 Heavy Chain 
       (VH/CH1/CH2/CH3)

<400>  55

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Val Gln Ser Gly Gly Gln Met Lys Lys Pro 
            20                  25                  30          


Gly Glu Ser Met Arg Ile Ser Cys Arg Ala Ser Gly Tyr Glu Phe Ile 
        35                  40                  45              


Asp Cys Thr Leu Asn Trp Ile Arg Leu Ala Pro Gly Lys Arg Pro Glu 
    50                  55                  60                  


Trp Met Gly Trp Leu Lys Pro Arg Gly Gly Ala Val Asn Tyr Ala Arg 
65                  70                  75                  80  


Pro Leu Gln Gly Arg Val Thr Met Thr Arg Asp Val Tyr Ser Asp Thr 
                85                  90                  95      


Ala Phe Leu Glu Leu Arg Ser Leu Thr Val Asp Asp Thr Ala Val Tyr 
            100                 105                 110         


Phe Cys Thr Arg Gly Lys Asn Cys Asp Tyr Asn Trp Asp Phe Glu His 
        115                 120                 125             


Trp Gly Arg Gly Thr Pro Val Ile Val Ser Ser Pro Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Ala Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            260                 265                 270         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        275                 280                 285             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    290                 295                 300                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
305                 310                 315                 320 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                325                 330                 335     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            340                 345                 350         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        355                 360                 365             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    370                 375                 380                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
385                 390                 395                 400 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                405                 410                 415     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            420                 425                 430         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        435                 440                 445             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    450                 455                 460                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470                 


<210>  56
<211>  708
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding the HIV-1 VRC01 IgG Light Chain 
       (VL/CL)

<400>  56
ggatccgcca ccatggattg gacttggatt ctgttcctgg tggcagccgc taccagagtc       60

cattccgaaa ttgtgctgac ccagtctccc ggaacactgt ctctgagtcc tggcgagaca      120

gccatcattt cctgtaggac ttctcagtac gggagtctgg catggtatca gcagcgacca      180

ggacaggctc ctcgactggt catctactca ggaagcactc gggcagccgg cattcccgac      240

cgattctccg ggtctcggtg gggacctgat tacaacctga ccatctcaaa tctggaaagc      300

ggagactttg gcgtgtacta ttgccagcag tatgagttct ttgggcaggg aaccaaggtc      360

caggtggaca tcaaacgcac agtcgctgca ccaagcgtgt tcatctttcc accctcagat      420

gaacagctga agtccggcac cgcctctgtg gtgtgcctgc tgaacaattt ctacccccgg      480

gaggcaaagg tccagtggaa agtggacaac gccctgcagt ctggcaatag tcaggagtca      540

gtgactgaac aggacagcaa ggattccacc tattctctgt cctctactct gaccctgagc      600

aaagctgatt acgagaagca caaagtgtat gcatgtgagg tcacccacca gggactgcgg      660

tcacccgtca ccaagagctt caatcgcgga gagtgttgat aactcgag                   708


<210>  57
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the HIV-1 VRC01 IgG Light Chain (VL/CL)

<400>  57

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser 
            20                  25                  30          


Pro Gly Glu Thr Ala Ile Ile Ser Cys Arg Thr Ser Gln Tyr Gly Ser 
        35                  40                  45              


Leu Ala Trp Tyr Gln Gln Arg Pro Gly Gln Ala Pro Arg Leu Val Ile 
    50                  55                  60                  


Tyr Ser Gly Ser Thr Arg Ala Ala Gly Ile Pro Asp Arg Phe Ser Gly 
65                  70                  75                  80  


Ser Arg Trp Gly Pro Asp Tyr Asn Leu Thr Ile Ser Asn Leu Glu Ser 
                85                  90                  95      


Gly Asp Phe Gly Val Tyr Tyr Cys Gln Gln Tyr Glu Phe Phe Gly Gln 
            100                 105                 110         


Gly Thr Lys Val Gln Val Asp Ile Lys Arg Thr Val Ala Ala Pro Ser 
        115                 120                 125             


Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala 
    130                 135                 140                 


Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val 
145                 150                 155                 160 


Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser 
                165                 170                 175     


Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr 
            180                 185                 190         


Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys 
        195                 200                 205             


Glu Val Thr His Gln Gly Leu Arg Ser Pro Val Thr Lys Ser Phe Asn 
    210                 215                 220                 


Arg Gly Glu Cys 
225             


<210>  58
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding the Heavy Chain (VH-CH1) of the 
       CHIKV-Env-Fab

<400>  58
ggatccgcca ccatggattg gacatggagg attctgtttc tggtcgccgc cgctactgga       60

actcacgctc aggtgcagct ggtgcagtca gggtccgaac tgaagaaacc aggggcatct      120

gtgaaggtca gttgcaaagc ctcaggctac accctgacac ggtatgccat gacttgggtg      180

cgccaggctc ctggacaggg actggagtgg atgggctgga tcaacactta caccggaaat      240

ccaacttatg tgcaggggtt caccggccga ttcgtgtttt ctctggacac ttccgtctct      300

accgcctttc tgcacattac aagtctgaag gcagaggaca ctgccgtgta cttctgcgct      360

agggaaggcg gagcaagagg ctttgattat tggggccagg gaaccctggt gacagtcagc      420

tccgccagca caaagggacc ctccgtgttc ccactggctc cctctagtaa aagtacatca      480

gggggcactg ccgctctggg atgtctggtc aaagattact tccccgaacc tgtgaccgtc      540

agctggaact ccggagctct gaccagcggg gtgcatacat ttcccgcagt cctgcagtca      600

agcggactgt actccctgtc ctctgtggtc acagtgccta gttcaagcct ggggacacag      660

acttatatct gtaatgtgaa ccataagcca agcaacacca aagtggacaa aaaagtggaa      720

cctaagagct gctgataact cgag                                             744


<210>  59
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the Heavy Chain (VH-CH1) of the 
       CHIKV-Env-Fab

<400>  59

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Gln Val Gln Leu Val Gln Ser Gly Ser Glu Leu Lys Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Leu 
        35                  40                  45              


Thr Arg Tyr Ala Met Thr Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Asn Pro Thr Tyr Val 
65                  70                  75                  80  


Gln Gly Phe Thr Gly Arg Phe Val Phe Ser Leu Asp Thr Ser Val Ser 
                85                  90                  95      


Thr Ala Phe Leu His Ile Thr Ser Leu Lys Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Glu Gly Gly Ala Arg Gly Phe Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


<210>  60
<211>  738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding the Light Chain (VL-CL) of the 
       CHIKV-Env-Fab

<400>  60
ggatccgcca ccatggcatg gaccccactg ttcctgttcc tgctgacttg ttgtcctggc       60

gggagcaatt cacagagcgt cctgacccag cccccttctg tgtccggagc accaggacag      120

cgagtcacaa tctcttgcac tggaagctcc tctaacattg gggccagcca cgacgtgcat      180

tggtaccagc agctgccagg gaccgctccc acactgctga tctatgtgaa ctctaatagg      240

cctagtggcg tcccagatag attttcaggg agcaagtccg gcacctctgc tagtctggca      300

attacaggac tgcaggctga ggacgaagca gattactatt gccagagtta cgactcaaac      360

ctgtcaggca gcgcagtgtt cggaggagga actaagctga ccgtcctggg acagcccaaa      420

gccgctcctt ctgtgaccct gtttccccct agttcagagg aactgcaggc caacaaggct      480

actctggtgt gtctgatctc cgacttctac cctggagcag tgaccgtcgc atggaaggcc      540

gatagctccc cagtgaaagc tggggtcgag accacaactc ccagcaagca gtccaacaac      600

aagtacgcag cctctagtta tctgtcactg acacctgaac agtggaagag ccacaaatcc      660

tattcttgcc aagtgactca tgagggcagt accgtggaaa agacagtcgc cccaactgag      720

tgttcctgat aactcgag                                                    738


<210>  61
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence of the Light Chain (VL-CL) of the 
       CHIKV-Env-Fab

<400>  61

Met Ala Trp Thr Pro Leu Phe Leu Phe Leu Leu Thr Cys Cys Pro Gly 
1               5                   10                  15      


Gly Ser Asn Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Gly 
            20                  25                  30          


Ala Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn 
        35                  40                  45              


Ile Gly Ala Ser His Asp Val His Trp Tyr Gln Gln Leu Pro Gly Thr 
    50                  55                  60                  


Ala Pro Thr Leu Leu Ile Tyr Val Asn Ser Asn Arg Pro Ser Gly Val 
65                  70                  75                  80  


Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala 
                85                  90                  95      


Ile Thr Gly Leu Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Ser 
            100                 105                 110         


Tyr Asp Ser Asn Leu Ser Gly Ser Ala Val Phe Gly Gly Gly Thr Lys 
        115                 120                 125             


Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe 
    130                 135                 140                 


Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys 
145                 150                 155                 160 


Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala 
                165                 170                 175     


Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys 
            180                 185                 190         


Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro 
        195                 200                 205             


Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys Gln Val Thr His Glu 
    210                 215                 220                 


Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
225                 230                 235             


<210>  62
<211>  2238
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding HIV-1 Env-4E10 Ig

<400>  62
ggatccgcca ccatggattg gacatggagg attctgtttc tggtcgccgc cgctacagga       60

actcacgccc aggtgcagct ggtgcagtca ggagccgaag tgaagcgacc aggcagctcc      120

gtcactgtgt cctgcaaagc atctggcgga tcattcagca cctacgccct gagctgggtg      180

agacaggctc ctggacgagg actggaatgg atgggaggcg tcatcccact gctgacaatt      240

actaactacg ccccccgatt tcagggcagg atcaccatta cagcagaccg ctccacttct      300

accgcctatc tggagctgaa tagcctgaga ccagaagata ccgcagtgta ctattgcgcc      360

cgggagggaa ccacaggatg gggatggctg ggaaagccca tcggggcttt cgcacactgg      420

ggccagggaa ccctggtcac agtgtctagt gccagcacaa agggcccctc cgtgtttccc      480

ctggctcctt caagcaaaag tacttcagga gggaccgccg ctctgggatg tctggtgaag      540

gactacttcc ctgagccagt caccgtgtcc tggaactctg gcgctctgac ctccggagtg      600

catacatttc ccgcagtcct gcagtcctct gggctgtact ctctgagttc agtggtcact      660

gtgcctagct cctctctggg cacacagact tatatctgca acgtgaatca caagccctcc      720

aataccaaag tcgacaagaa agtggaacct aagtcttgtg ataaaaccca tacatgccca      780

ccttgtccag cacctgagct gctgggcgga ccttccgtgt tcctgtttcc acccaagcca      840

aaagacacac tgatgattag ccggacacct gaagtgactt gtgtggtcgt ggacgtcagc      900

cacgaggacc ccgaagtgaa gttcaactgg tacgtggatg gcgtcgaggt gcataatgcc      960

aagaccaaac ccagggagga acagtacaac tctacttata gggtcgtgag tgtcctgacc     1020

gtgctgcacc aggactggct gaacgggaag gagtataagt gcaaagtgtc caataaggcc     1080

ctgccagctc ccatcgagaa aacaatttct aaggctaaag gccagccacg cgaaccccag     1140

gtgtacactc tgcctcccag cagggacgag ctgaccaaga accaggtgag tctgacatgt     1200

ctggtcaaag gcttctatcc aagcgatatc gccgtggagt gggaatccaa tggacagccc     1260

gaaaacaatt acaagactac cccccctgtg ctggacagtg atggatcatt ctttctgtat     1320

tccaagctga ccgtggacaa atctcgctgg cagcagggga acgtctttag ctgctccgtg     1380

atgcacgagg ccctgcacaa tcattacaca cagaagtctc tgagtctgtc accaggcaag     1440

cggggacgca aaaggagaag cgggtccggc gctactaact tcagcctgct gaaacaggca     1500

ggggatgtgg aggaaaatcc tggcccaatg gtcctgcaga cccaggtgtt tatctcactg     1560

ctgctgtgga ttagcggggc ttatggcgaa atcgtgctga ctcagagccc cggaacccag     1620

tctctgagtc ctggggagcg cgctacactg agctgtcgag catcacagag cgtggggaac     1680

aataagctgg catggtacca gcagaggcct ggccaggctc caagactgct gatctatggc     1740

gcaagttcac ggcctagcgg agtggcagac cgcttctccg gatctgggag tggcaccgat     1800

tttactctga ccattagcag gctggagcca gaagacttcg ctgtgtacta ttgccagcag     1860

tacggccagt cactgagcac atttggacag gggactaagg tcgaaaaaag aaccgtggca     1920

gccccaagtg tcttcatttt tccaccctca gacgagcagc tgaagagtgg aacagcctca     1980

gtcgtgtgtc tgctgaacaa tttctacccc agggaggcca aggtccagtg gaaagtggat     2040

aacgctctgc agagcggcaa ttcccaggag tctgtgacag aacaggacag taaggattca     2100

acttatagcc tgagctccac actgactctg tccaaagcag attacgagaa gcacaaagtg     2160

tatgcctgcg aagtcaccca tcagggactg tctagtcctg tgacaaagtc ttttaacaga     2220

ggggagtgat aactcgag                                                   2238


<210>  63
<211>  2328
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding HIV-1 Env-PG9 Ig

<400>  63
ggatccgcca ccatggactg gacttggagg attctgtttc tggtcgccgc cgcaactgga       60

actcacgctg aatttggact gtcatgggtc tttctggtgg cctttctgcg aggggtccag      120

tgccagaggc tggtggagtc cggaggagga gtggtccagc caggcagctc cctgcgactg      180

agttgtgccg cttcagggtt cgacttttct agacagggca tgcactgggt gcggcaggca      240

ccaggacagg gactggagtg ggtggctttc atcaagtacg acggaagtga aaaatatcat      300

gccgattcag tgtgggggcg gctgtcaatt agccgcgaca actccaagga taccctgtac      360

ctgcagatga attctctgag ggtcgaggac acagctactt atttctgcgt gagggaagca      420

ggcggacctg attacagaaa cgggtataat tactatgact tttacgatgg ctactataac      480

taccactata tggacgtgtg gggcaaggga accacagtca cagtgtctag tgcatcaact      540

aaaggcccaa gcgtgtttcc cctggcccct tcaagcaagt ccacttctgg aggaaccgca      600

gcactgggat gtctggtgaa ggattacttc cctgagccag tcaccgtgag ttggaactca      660

ggcgccctga ctagcggagt ccataccttt cctgctgtgc tgcagtcctc tgggctgtac      720

agcctgagtt cagtggtcac agtgccaagc tcctctctgg gcacccagac atatatctgc      780

aacgtgaatc acaagcctag caatactaag gtcgacaaaa gagtggaacc aaagagctgt      840

gataaaactc atacctgccc accttgtcca gcacctgagc tgctgggagg gccttccgtg      900

ttcctgtttc cacccaagcc aaaagacacc ctgatgatta gccggacacc agaagtcact      960

tgcgtggtcg tggacgtgag ccacgaggac cccgaagtca agtttaactg gtacgtggat     1020

ggcgtcgagg tgcataatgc taagacaaaa ccacgggagg aacagtacaa ctccacatat     1080

cgcgtcgtgt ctgtcctgac tgtgctgcac caggactggc tgaacggcaa ggagtataag     1140

tgcaaagtgt ccaataaggc actgccagcc cccatcgaga aaaccatttc taaggccaaa     1200

ggccagccac gagaacccca ggtgtacaca ctgcctccaa gtagggacga gctgactaag     1260

aaccaggtct ctctgacctg tctggtgaaa ggcttctatc cctctgatat cgctgtggag     1320

tgggaaagta atggacagcc tgaaaacaat tacaagacta ccccccctgt gctggacagc     1380

gatggcagct tcttcctgta tagcaagctg accgtggaca aatccagatg gcagcagggg     1440

aacgtcttta gttgctcagt gatgcacgag gcactgcaca atcattacac ccagaaaagc     1500

ctgtccctgt ctcctggcaa gaggggaaga aaaaggagaa gtgggtcagg cgcaacaaac     1560

ttcagcctgc tgaagcaggc cggagatgtg gaggaaaatc ctgggccaat ggcttggacc     1620

cccctgttcc tgtttctgct gacatgctgt cctggcggaa gcaactccca gtctgcactg     1680

acacagccag caagtgtgtc agggagccca ggacagagca tcaccatttc ctgtaacggc     1740

acaagcaatg acgtcggggg ctacgagtcc gtgtcttggt atcagcagca tcctggaaag     1800

gccccaaaag tcgtgatcta cgatgtcagc aaacgcccct ctggggtgag taaccgattc     1860

agtggatcaa agagcgggaa taccgcttct ctgacaatta gtggcctgca ggcagaggac     1920

gaaggagatt actattgcaa atcactgaca agcactcggc gccgagtctt cggaaccggg     1980

acaaagctga ctgtgctggg ccagcccaaa gctgcaccta gcgtgaccct gtttccaccc     2040

agttcagagg aactgcaggc taataaggca acactggtgt gtctgatctc cgacttctac     2100

cctggcgctg tcactgtggc ctggaaggct gatagctccc cagtcaaagc aggagtggaa     2160

acaactaccc cctccaagca gtctaacaac aagtacgccg cttctagtta tctgtcactg     2220

actcccgagc agtggaagag ccacaaatcc tattcttgcc aggtgaccca tgagggctcc     2280

actgtcgaaa agaccgtggc ccctacagag tgttcttgat aactcgag                  2328


<210>  64
<211>  2217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid Sequence Encoding VRC01 IgG

<400>  64
ggatccgcca ccatggattg gacatggatt ctgttcctgg tcgccgccgc aactagagtg       60

cattcacagg tgcagctggt gcagtcaggc gggcagatga agaaacccgg cgagagtatg      120

cgaatctcat gccgggctag cgggtacgaa ttcatcgact gtaccctgaa ctggattaga      180

ctggcacctg ggaagaggcc agagtggatg ggatggctga aacctagagg cggggcagtg      240

aattacgcca gaccactgca gggcagggtc actatgaccc gcgacgtgta ttctgatacc      300

gcattcctgg agctgcgaag tctgacagtc gacgatactg ccgtgtactt ctgcacacgg      360

ggcaagaact gtgactataa ttgggatttt gaacactggg gcagggggac acctgtcatt      420

gtgagctccc caagtactaa gggaccctca gtgtttcccc tggccccttc tagtaaaagt      480

acctcaggag gcacagccgc tctgggatgc ctggtgaagg attacttccc tgagccagtc      540

accgtgagtt ggaactcagg cgccctgaca agcggggtcc atacttttcc agctgtgctg      600

cagtcaagcg ggctgtactc cctgtcctct gtggtcacag tgcccagttc aagcctggga      660

acacagactt atatctgtaa cgtcaatcac aagcctagca atactaaagt ggacaagaaa      720

gccgagccta agagctgcga accaaagtcc tgtgataaaa cccatacatg ccctccctgt      780

ccagctcctg aactgctggg cggcccatcc gtgttcctgt ttccacccaa gcccaaagac      840

accctgatga ttagcaggac tcctgaggtc acctgcgtgg tcgtggacgt gtcccacgag      900

gaccccgaag tcaagtttaa ctggtacgtg gatggcgtcg aagtgcataa tgccaagaca      960

aaaccccggg aggaacagta caactctacc tatagagtcg tgagtgtcct gacagtgctg     1020

caccaggact ggctgaacgg gaaggagtat aagtgcaaag tgtctaataa ggccctgcca     1080

gctcccatcg agaaaacaat ttccaaggca aaaggccagc caagggaacc ccaggtgtac     1140

actctgcctc catcccgcga cgagctgact aagaaccagg tctctctgac ctgtctggtg     1200

aaaggattct atccaagcga tatcgccgtg gagtgggaat ccaatggcca gcccgagaac     1260

aattacaaga ccacaccccc tgtgctggac agcgatggct ccttctttct gtattcaaag     1320

ctgaccgtgg ataaaagccg ctggcagcag gggaacgtct ttagctgctc cgtgatgcac     1380

gaagctctgc acaatcatta cacccagaag tctctgagtc tgtcacctgg caagagggga     1440

cgaaaacgga gaagcggcag cggagctaca aacttcagcc tgctgaaaca ggcaggcgac     1500

gtggaggaaa atcctgggcc aatggattgg acttggattc tgttcctggt ggcagccgct     1560

accagagtcc attccgaaat tgtgctgacc cagtctcccg gaacactgtc tctgagtcct     1620

ggcgagacag ccatcatttc ctgtaggact tctcagtacg ggagtctggc atggtatcag     1680

cagcgaccag gacaggctcc tcgactggtc atctactcag gaagcactcg ggcagccggc     1740

attcccgacc gattctccgg gtctcggtgg ggacctgatt acaacctgac catctcaaat     1800

ctggaaagcg gagactttgg cgtgtactat tgccagcagt atgagttctt tgggcaggga     1860

accaaggtcc aggtggacat caaacgcaca gtcgctgcac caagcgtgtt catctttcca     1920

ccctcagatg aacagctgaa gtccggcacc gcctctgtgg tgtgcctgct gaacaatttc     1980

tacccccggg aggcaaaggt ccagtggaaa gtggacaacg ccctgcagtc tggcaatagt     2040

caggagtcag tgactgaaca ggacagcaag gattccacct attctctgtc ctctactctg     2100

accctgagca aagctgatta cgagaagcac aaagtgtatg catgtgaggt cacccaccag     2160

ggactgcggt cacccgtcac caagagcttc aatcgcggag agtgttgata actcgag        2217


<210>  65
<211>  2223
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CHIKV snapi nucleic acid sequence

<400>  65
ggatccgcca ccatggactg gacttggatt ctgtttctgg tcgccgccgc tacccgagtg       60

cattcacagg tgcagctgca gcagcctggg gccgctctgg tgaagccagg agctagcgca      120

atgatgtcct gcaaagcctc tggctacact ttcacctcct attggatcac ctgggtgaag      180

cagcgacctg gacagggact ggagtggatc ggcgacatct acccaggcac cgggagaaca      240

atctacaagg aaaaattcaa gacaaaagcc acactgactg tggacaccag ctcctctaca      300

gcttttatgc agctgaacag cctgacttcc gaggatagcg ccgtgtacta ttgcgcaaga      360

ggatacggct ctccttacta tgccctggac tattgggggc agggaactag cgtcaccgtg      420

agttcagcat ctaccaaggg accaagcgtg ttcccactgg cacctagctc caaatccact      480

tctggcggga ccgccgctct gggatgtctg gtgaaggatt acttccctga gccagtcaca      540

gtgagttgga actcaggggc tctgaccagc ggagtccaca catttcctgc agtgctgcag      600

tctagtggac tgtactccct gtcaagcgtg gtcactgtcc catcctctag tctgggcacc      660

cagacatata tctgcaacgt gaatcacaag ccatccaata ccaaagtcga taagaaagtg      720

gagcccaagt cttgtgacaa aactcatacc tgccctccct gtccagcacc tgaactgctg      780

ggaggcccaa gcgtgttcct gtttccaccc aagcctaaag acaccctgat gattagcagg      840

acaccagagg tcacttgcgt ggtcgtggac gtgagccacg aagaccccga ggtcaagttc      900

aactggtacg tggatggcgt cgaagtgcat aatgccaaga caaaaccccg ggaggaacag      960

tacaactcaa cctatcgggt cgtgagcgtc ctgacagtgc tgcaccagga ctggctgaac     1020

ggaaaggagt acaagtgcaa agtgtctaat aaggccctgc cagctcccat cgaaaaaacc     1080

attagcaagg ctaaaggcca gccaagagag ccccaggtgt acacactgcc tccatcaagg     1140

gacgaactga caaagaacca ggtcagcctg acttgtctgg tgaaaggctt ctatcccagc     1200

gatatcgcag tggaatggga gtccaatggg cagcctgaga acaattacaa gaccacaccc     1260

cctgtgctgg acagcgatgg gtccttcttt ctgtattcca agctgacagt ggataaatct     1320

cggtggcagc agggaaacgt ctttagttgc tcagtgatgc acgaagccct gcacaatcat     1380

tacactcaga agagcctgtc cctgtctccc ggaaagaggg gccgcaaacg gagaagtggc     1440

tcaggggcaa ccaacttctc tctgctgaaa caggccggcg atgtggagga aaatcctggg     1500

ccaatggact ggacatggat tctgttcctg gtggcagccg ctacaagggt ccattccgac     1560

attgtgctga ctcagtctcc tgcaagtctg gccgtgtctc agggacagcg agcaaccatc     1620

agttgtaagg ctagccagtc cgtcgactac gatggggaca gttacgtgaa ctggtatcag     1680

cagaagcctg gacagtcccc aaaactgctg atctatgatg ctagtaatct ggagtcaggc     1740

attcccgcac gattctctgg aagtggctca gggacagact tcaccctgaa cattcaccct     1800

gtcgaggaag aggacgtggc tacctactat tgccaggaaa gcaatgagga cccccgcact     1860

ttcgggggag gcaccaagct ggagatcaaa cgaactgtcg cagcccccag cgtgttcatc     1920

tttccaccct cagacgaaca gctgaagagc ggaaccgcat ccgtggtgtg cctgctgaac     1980

aacttctacc cccgcgaggc caaggtccag tggaaagtgg ataacgctct gcagtcaggc     2040

aatagccagg aatccgtgac tgagcaggat tctaaggaca gtacctattc actgtcaagc     2100

acactgactc tgagcaaagc agactacgaa aagcataaag tgtatgcctg cgaagtcacc     2160

caccaggggc tgaggtctcc agtcactaag tctttcaaca gaggggaatg ctgataactc     2220

gag                                                                   2223


<210>  66
<211>  733
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CHIKV-snapi amino acid sequence

<400>  66

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Ala Leu Val Lys Pro 
            20                  25                  30          


Gly Ala Ser Ala Met Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
        35                  40                  45              


Ser Tyr Trp Ile Thr Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
    50                  55                  60                  


Trp Ile Gly Asp Ile Tyr Pro Gly Thr Gly Arg Thr Ile Tyr Lys Glu 
65                  70                  75                  80  


Lys Phe Lys Thr Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr 
                85                  90                  95      


Ala Phe Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Gly Tyr Gly Ser Pro Tyr Tyr Ala Leu Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 
    130                 135                 140                 


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
145                 150                 155                 160 


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
                165                 170                 175     


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
            180                 185                 190         


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
        195                 200                 205             


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
    210                 215                 220                 


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser 
225                 230                 235                 240 


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
                245                 250                 255     


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
305                 310                 315                 320 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
        355                 360                 365             


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
    370                 375                 380                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
385                 390                 395                 400 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                405                 410                 415     


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            420                 425                 430         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        435                 440                 445             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    450                 455                 460                 


Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser Gly Ser Gly Ala Thr 
465                 470                 475                 480 


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
                485                 490                 495     


Pro Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg 
            500                 505                 510         


Val His Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
        515                 520                 525             


Ser Gln Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
    530                 535                 540                 


Asp Tyr Asp Gly Asp Ser Tyr Val Asn Trp Tyr Gln Gln Lys Pro Gly 
545                 550                 555                 560 


Gln Ser Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Glu Ser Gly 
                565                 570                 575     


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
            580                 585                 590         


Asn Ile His Pro Val Glu Glu Glu Asp Val Ala Thr Tyr Tyr Cys Gln 
        595                 600                 605             


Glu Ser Asn Glu Asp Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu 
    610                 615                 620                 


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
625                 630                 635                 640 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
                645                 650                 655     


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
            660                 665                 670         


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
        675                 680                 685             


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
    690                 695                 700                 


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
705                 710                 715                 720 


Arg Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
                725                 730             


<210>  67
<211>  2241
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DVSF-1 WT nucleic acid sequence

<400>  67
ggatccgcca ccatggactg gacttggagg attctgtttc tggtcgccgc cgctactggg       60

actcacgctc aggcacatct ggtcgaatct ggaggaggag tggtccagcc tggccgatcc      120

ctgcgactgt cttgcgcagc tagcgccttc aacttcagca caaacgcaat gcactgggtg      180

cgacaggcac caggcaaggg actggagtgg gtcgctgtga tctcatacga cggaagccat      240

aagtactatg cagattctgt gaaaggccgg ttcaccattt ccagggacaa ttctaagaac      300

accctgtatc tgcagatgaa tagcctgcgc gcagccgata ccgcagtgta ctattgcgca      360

actgtcggcg tgctgacctg gccagtgaac gccgaatact ttcaccattg gggacagggc      420

agtctggtct cagtgagctc cgcaagtact aagggaccat cagtgttccc actggcaccc      480

tctagtaaat ctactagtgg cgggaccgct gcactgggat gtctggtgaa ggactatttc      540

cccgagcctg tcaccgtgag ctggaattcc ggagccctga caagcggcgt ccacactttt      600

cccgctgtgc tgcagtcaag cggactgtac tccctgtcct ctgtggtcac tgtgcctagt      660

tcaagcctgg gcactcagac ctatatctgc aatgtgaacc acaagccctc taacaccaaa      720

gtcgacaaga aagtggaacc taagagctgt gataaaacac atacttgccc accttgtcca      780

gcaccagagc tgctgggagg accaagcgtg ttcctgtttc cacccaagcc taaagacaca      840

ctgatgatta gccggacacc tgaagtcact tgcgtggtcg tggacgtgtc ccacgaggac      900

cccgaagtca agtttaattg gtacgtggat ggcgtcgagg tgcataacgc caagaccaaa      960

ccccgggagg aacagtacaa tagcacatat agagtcgtgt ccgtcctgac tgtgctgcat     1020

caggattggc tgaatgggaa ggagtataag tgcaaagtgt ctaacaaggc tctgcctgca     1080

ccaatcgaga aaaccattag caaggctaaa ggccagccta gggaaccaca ggtgtacaca     1140

ctgcctccaa gtcgcgacga gctgaccaag aatcaggtct ccctgacatg tctggtgaaa     1200

ggcttctatc catcagatat cgccgtggag tgggaaagca acgggcagcc cgaaaacaat     1260

tacaagacca caccccctgt gctggactct gatggcagtt tctttctgta ttctaagctg     1320

accgtggaca aaagtagatg gcagcagggg aatgtctttt catgtagcgt gatgcacgag     1380

gccctgcaca accattacac acagaagtcc ctgtctctga gtcccggaaa gaggggccgc     1440

aaacggagat cagggagcgg agctactaat ttcagcctgc tgaaacaggc aggggatgtg     1500

gaggaaaacc ccggacctat ggcttggacc ccactgttcc tgtttctgct gacatgctgt     1560

cccgggggca gcaattctca gagtgtcctg acacagccac catcagtgag cggagcacca     1620

ggacagaggg tgaccatctc ctgcacaggc agcagcagca acattggcgc cgggtacgac     1680

gtgcattggt atcagcagct gcccggcacc gctcctaagc tgctgatctg tggcaacaat     1740

aaccgcccat ctggggtgcc cgatcgattc tccggctcta aaagtgggac ttcagccagc     1800

ctggctatta ccggcctgca ggccgaggac gaagctgatt actattgcca gagctacgac     1860

tcaagcctga ccggagtcgt gttcggagga ggaaccaagc tgacagtcct gggacagcct     1920

aaagccgctc caagcgtgac actgtttcct ccatcctctg aggaactgca ggcaaacaag     1980

gccaccctgg tgtgcctgat ttccgacttc taccccgggg cagtcactgt ggcttggaag     2040

gcagatagtt cacctgtcaa agccggagtg gagactacca caccatcaaa gcagagcaat     2100

aacaaatacg cagccagctc ctatctgtcc ctgacccctg agcagtggaa gtctcacaaa     2160

tcctattctt gccaggtcac tcacgaagga agcactgtgg agaaaactgt cgcaccaacc     2220

gaatgtagtt gataactcga g                                               2241


<210>  68
<211>  739
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DVSF-1 WT amino acid sequence

<400>  68

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Gln Ala His Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Ala Phe Asn Phe 
        35                  40                  45              


Ser Thr Asn Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser His Lys Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Val Gly Val Leu Thr Trp Pro Val Asn Ala Glu 
        115                 120                 125             


Tyr Phe His His Trp Gly Gln Gly Ser Leu Val Ser Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser 
465                 470                 475                 480 


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
                485                 490                 495     


Glu Glu Asn Pro Gly Pro Met Ala Trp Thr Pro Leu Phe Leu Phe Leu 
            500                 505                 510         


Leu Thr Cys Cys Pro Gly Gly Ser Asn Ser Gln Ser Val Leu Thr Gln 
        515                 520                 525             


Pro Pro Ser Val Ser Gly Ala Pro Gly Gln Arg Val Thr Ile Ser Cys 
    530                 535                 540                 


Thr Gly Ser Ser Ser Asn Ile Gly Ala Gly Tyr Asp Val His Trp Tyr 
545                 550                 555                 560 


Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Cys Gly Asn Asn 
                565                 570                 575     


Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly 
            580                 585                 590         


Thr Ser Ala Ser Leu Ala Ile Thr Gly Leu Gln Ala Glu Asp Glu Ala 
        595                 600                 605             


Asp Tyr Tyr Cys Gln Ser Tyr Asp Ser Ser Leu Thr Gly Val Val Phe 
    610                 615                 620                 


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro 
625                 630                 635                 640 


Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys 
                645                 650                 655     


Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr 
            660                 665                 670         


Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr 
        675                 680                 685             


Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr 
    690                 695                 700                 


Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys 
705                 710                 715                 720 


Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr 
                725                 730                 735     


Glu Cys Ser 
            


<210>  69
<211>  2241
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DVSF-1 LALA nucleic acid sequence

<400>  69
ggatccgcca ccatggactg gacttggagg attctgtttc tggtcgccgc cgctactggg       60

actcacgctc aggcacatct ggtcgaatct ggaggaggag tggtccagcc tggccgatcc      120

ctgcgactgt cttgcgcagc tagcgccttc aacttcagca caaacgcaat gcactgggtg      180

cgacaggcac caggcaaggg actggagtgg gtcgctgtga tctcatacga cggaagccat      240

aagtactatg cagattctgt gaaaggccgg ttcaccattt ccagggacaa ttctaagaac      300

accctgtatc tgcagatgaa tagcctgcgc gcagccgata ccgcagtgta ctattgcgca      360

actgtcggcg tgctgacctg gccagtgaac gccgaatact ttcaccattg gggacagggc      420

agtctggtct cagtgagctc cgcaagtact aagggaccat cagtgttccc actggcaccc      480

tctagtaaat ctactagtgg cgggaccgct gcactgggat gtctggtgaa ggactatttc      540

cccgagcctg tcaccgtgag ctggaattcc ggagccctga caagcggcgt ccacactttt      600

cccgctgtgc tgcagtcaag cggactgtac tccctgtcct ctgtggtcac tgtgcctagt      660

tcaagcctgg gcactcagac ctatatctgc aatgtgaacc acaagccctc taacaccaaa      720

gtcgacaaga aagtggaacc taagagctgt gataaaacac atacttgccc accttgtcca      780

gcaccagagg cagctggagg accaagcgtg ttcctgtttc cacccaagcc taaagacaca      840

ctgatgatta gccggacacc tgaagtcact tgcgtggtcg tggacgtgtc ccacgaggac      900

cccgaagtca agtttaattg gtacgtggat ggcgtcgagg tgcataacgc caagaccaaa      960

ccccgggagg aacagtacaa tagcacatat agagtcgtgt ccgtcctgac tgtgctgcat     1020

caggattggc tgaatgggaa ggagtataag tgcaaagtgt ctaacaaggc tctgcctgca     1080

ccaatcgaga aaaccattag caaggctaaa ggccagccta gggaaccaca ggtgtacaca     1140

ctgcctccaa gtcgcgacga gctgaccaag aatcaggtct ccctgacatg tctggtgaaa     1200

ggcttctatc catcagatat cgccgtggag tgggaaagca acgggcagcc cgaaaacaat     1260

tacaagacca caccccctgt gctggactct gatggcagtt tctttctgta ttctaagctg     1320

accgtggaca aaagtagatg gcagcagggg aatgtctttt catgtagcgt gatgcacgag     1380

gccctgcaca accattacac acagaagtcc ctgtctctga gtcccggaaa gaggggccgc     1440

aaacggagat cagggagcgg agctactaat ttcagcctgc tgaaacaggc aggggatgtg     1500

gaggaaaacc ccggacctat ggcttggacc ccactgttcc tgtttctgct gacatgctgt     1560

cccgggggca gcaattctca gagtgtcctg acacagccac catcagtgag cggagcacca     1620

ggacagaggg tgaccatctc ctgcacaggc agcagcagca acattggcgc cgggtacgac     1680

gtgcattggt atcagcagct gcccggcacc gctcctaagc tgctgatctg tggcaacaat     1740

aaccgcccat ctggggtgcc cgatcgattc tccggctcta aaagtgggac ttcagccagc     1800

ctggctatta ccggcctgca ggccgaggac gaagctgatt actattgcca gagctacgac     1860

tcaagcctga ccggagtcgt gttcggagga ggaaccaagc tgacagtcct gggacagcct     1920

aaagccgctc caagcgtgac actgtttcct ccatcctctg aggaactgca ggcaaacaag     1980

gccaccctgg tgtgcctgat ttccgacttc taccccgggg cagtcactgt ggcttggaag     2040

gcagatagtt cacctgtcaa agccggagtg gagactacca caccatcaaa gcagagcaat     2100

aacaaatacg cagccagctc ctatctgtcc ctgacccctg agcagtggaa gtctcacaaa     2160

tcctattctt gccaggtcac tcacgaagga agcactgtgg agaaaactgt cgcaccaacc     2220

gaatgtagtt gataactcga g                                               2241


<210>  70
<211>  739
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DVSF-1 LALA amino acid sequence

<400>  70

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Gln Ala His Leu Val Glu Ser Gly Gly Gly Val Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Ala Phe Asn Phe 
        35                  40                  45              


Ser Thr Asn Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser His Lys Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Val Gly Val Leu Thr Trp Pro Val Asn Ala Glu 
        115                 120                 125             


Tyr Phe His His Trp Gly Gln Gly Ser Leu Val Ser Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser 
465                 470                 475                 480 


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
                485                 490                 495     


Glu Glu Asn Pro Gly Pro Met Ala Trp Thr Pro Leu Phe Leu Phe Leu 
            500                 505                 510         


Leu Thr Cys Cys Pro Gly Gly Ser Asn Ser Gln Ser Val Leu Thr Gln 
        515                 520                 525             


Pro Pro Ser Val Ser Gly Ala Pro Gly Gln Arg Val Thr Ile Ser Cys 
    530                 535                 540                 


Thr Gly Ser Ser Ser Asn Ile Gly Ala Gly Tyr Asp Val His Trp Tyr 
545                 550                 555                 560 


Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Cys Gly Asn Asn 
                565                 570                 575     


Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly 
            580                 585                 590         


Thr Ser Ala Ser Leu Ala Ile Thr Gly Leu Gln Ala Glu Asp Glu Ala 
        595                 600                 605             


Asp Tyr Tyr Cys Gln Ser Tyr Asp Ser Ser Leu Thr Gly Val Val Phe 
    610                 615                 620                 


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro 
625                 630                 635                 640 


Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys 
                645                 650                 655     


Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr 
            660                 665                 670         


Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr 
        675                 680                 685             


Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr 
    690                 695                 700                 


Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys 
705                 710                 715                 720 


Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr 
                725                 730                 735     


Glu Cys Ser 
            


<210>  71
<211>  2241
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DVSF-2 WT nucleic acid sequence

<400>  71
ggatccgcca ccatggactg gacatggaga atcctgttcc tggtcgccgc cgcaaccggg       60

acacacgccg aagtgcagct ggtggaatct ggagggggat gggtgcagcc aggagggtcc      120

ctgcgactgt cttgcgccgc tagtggcttc actttttcca gatacgacat gcactgggtc      180

aggcaggtga ccggaaaggg cctggaatgg gtgagcgcaa tcaccacagc cggagacaca      240

tactatcccg attctgtgaa gggccggttc accattagtc gggagaacgc caaaagctcc      300

ctgtatctgc agatgaacaa tctgagagct ggcgacaccg cactgtacta ttgcgctagg      360

ggccccccta cagattgctc tagtggacga tgtctgggag tcggagtggg actggaccca      420

tgggggcagg gaacactggt cactgtgtca agcgcctcca caaagggacc ctctgtgttc      480

cctctggctc catcctctaa aagtacttca ggaggaaccg cagcactggg atgtctggtg      540

aaggattact tcccagagcc cgtcaccgtg agctggaact ccggagctct gactagcggc      600

gtccatacct ttcctgcagt gctgcagagt tcaggcctgt acagcctgag ctccgtggtc      660

accgtgccat ctagttcact ggggacccag acatatatct gcaacgtgaa tcacaagcca      720

tctaatacaa aagtcgacaa gaaagtggaa cccaagagtt gtgataaaac tcatacctgc      780

ccaccatgtc ctgcaccaga gctgctggga ggaccatccg tgttcctgtt tcctccaaag      840

cccaaagaca cactgatgat tagcaggaca cccgaagtca cttgcgtggt cgtggacgtg      900

agccacgagg accccgaagt caagtttaac tggtacgtgg atggcgtcga ggtgcataat      960

gccaagacca aaccccggga ggaacagtac aacagtacct atagagtcgt gtcagtcctg     1020

acagtgctgc accaggactg gctgaacggg aaagagtata agtgcaaagt gtccaataag     1080

gcactgcccg cccctatcga gaaaaccatt tctaaggcca aaggacagcc ccgagaacct     1140

caggtgtaca cactgccccc tagccgcgac gagctgacaa agaaccaggt ctccctgact     1200

tgtctggtga aagggttcta tccttcagat atcgccgtgg agtgggaaag caatggacag     1260

ccagaaaaca attacaagac taccccaccc gtgctggact ctgatggcag tttctttctg     1320

tatagcaagc tgaccgtgga caaatcccgc tggcagcagg ggaacgtctt tagctgctcc     1380

gtgatgcatg aggccctgca caatcattac actcagaagt ctctgagtct gtcacctgga     1440

aagaggggac gaaaacgaag aagcggctcc ggagcaacca acttcagcct gctgaaacag     1500

gccggggatg tggaggaaaa tccaggaccc atggcatgga ctcctctgtt cctgtttctg     1560

ctgacctgct gtccaggcgg gagcaacagc tcctacgagg tgacccagcc tccatctgtc     1620

agtgtgtcac ccggccagac cgcttcaatc acatgtagcg gggacaagct gggaaagaaa     1680

tacacaagtt ggtatcagca gaaaccagga cagtcacccc tgctggtcat ctaccaggat     1740

actaagcgcc ctagcggcat tccagaacgg ttcagcggct ccaactctgg gaatacagct     1800

actctgacca tctccggcac ccaggccatg gacgaggctg attactattg ccaggcatgg     1860

gattctacaa ctcacgtcat tttcggaggc gggaccaagc tgacagtgct ggggcagccc     1920

aaagctgcac ctagcgtcac cctgtttccc ccttctagtg aggaactgca ggctaataag     1980

gcaacactgg tgtgtctgat ttccgacttc tacccaggag cagtcactgt ggcatggaag     2040

gctgattcaa gccccgtcaa agccggagtg gaaaccacaa ctccttcaaa gcagagcaac     2100

aacaagtacg ccgcttcctc ttatctgtcc ctgactcccg agcagtggaa gtctcacaaa     2160

agttattcat gccaggtgac ccatgagggc tccactgtcg aaaagaccgt ggcccctaca     2220

gagtgttctt gataactcga g                                               2241


<210>  72
<211>  739
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DVSF-2 WT amino acid sequence

<400>  72

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Trp Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Arg Tyr Asp Met His Trp Val Arg Gln Val Thr Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Ala Ile Thr Thr Ala Gly Asp Thr Tyr Tyr Pro Asp 
65                  70                  75                  80  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Lys Ser Ser 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Asn Leu Arg Ala Gly Asp Thr Ala Leu Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Gly Pro Pro Thr Asp Cys Ser Ser Gly Arg Cys Leu 
        115                 120                 125             


Gly Val Gly Val Gly Leu Asp Pro Trp Gly Gln Gly Thr Leu Val Thr 
    130                 135                 140                 


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
145                 150                 155                 160 


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
                165                 170                 175     


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
            180                 185                 190         


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
        195                 200                 205             


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
    210                 215                 220                 


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
225                 230                 235                 240 


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
            260                 265                 270         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
        275                 280                 285             


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
    290                 295                 300                 


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
305                 310                 315                 320 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
                325                 330                 335     


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
            340                 345                 350         


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
        355                 360                 365             


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
    370                 375                 380                 


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
385                 390                 395                 400 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
                405                 410                 415     


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
            420                 425                 430         


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
        435                 440                 445             


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
    450                 455                 460                 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg 
465                 470                 475                 480 


Lys Arg Arg Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln 
                485                 490                 495     


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala Trp Thr Pro Leu 
            500                 505                 510         


Phe Leu Phe Leu Leu Thr Cys Cys Pro Gly Gly Ser Asn Ser Ser Tyr 
        515                 520                 525             


Glu Val Thr Gln Pro Pro Ser Val Ser Val Ser Pro Gly Gln Thr Ala 
    530                 535                 540                 


Ser Ile Thr Cys Ser Gly Asp Lys Leu Gly Lys Lys Tyr Thr Ser Trp 
545                 550                 555                 560 


Tyr Gln Gln Lys Pro Gly Gln Ser Pro Leu Leu Val Ile Tyr Gln Asp 
                565                 570                 575     


Thr Lys Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser Asn Ser 
            580                 585                 590         


Gly Asn Thr Ala Thr Leu Thr Ile Ser Gly Thr Gln Ala Met Asp Glu 
        595                 600                 605             


Ala Asp Tyr Tyr Cys Gln Ala Trp Asp Ser Thr Thr His Val Ile Phe 
    610                 615                 620                 


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro 
625                 630                 635                 640 


Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys 
                645                 650                 655     


Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr 
            660                 665                 670         


Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr 
        675                 680                 685             


Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr 
    690                 695                 700                 


Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys 
705                 710                 715                 720 


Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr 
                725                 730                 735     


Glu Cys Ser 
            


<210>  73
<211>  2241
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DVSF-2 LALA nucleic acid sequence

<400>  73
ggatccgcca ccatggactg gacatggaga atcctgttcc tggtcgccgc cgctactggg       60

actcacgccg aagtgcagct ggtcgagagt ggagggggat gggtgcagcc cggcggcagc      120

ctgaggctgt cttgcgccgc tagtggcttc actttttcta gatacgacat gcactgggtc      180

cggcaggtga ccgggaaggg actggaatgg gtgagcgcca tcaccacagc aggggacaca      240

tactatcccg attctgtgaa gggcaggttc accattagta gggagaacgc aaaaagctcc      300

ctgtatctgc agatgaacaa tctgagagcc ggcgacaccg ctctgtacta ttgcgccagg      360

ggccctccca cagattgctc tagtggacgc tgtctgggag tcggagtggg actggaccca      420

tggggacagg ggacactggt caccgtgagc agcgcctcca ctaagggacc aagcgtgttc      480

cctctggcac catcctctaa aagtacttca gggggcaccg cagccctggg atgtctggtg      540

aaggattact tcccagagcc cgtcacagtg agctggaact ccggggccct gacttccgga      600

gtccacacct ttcctgctgt gctgcagagt tcaggcctgt actctctgag ctccgtggtc      660

acagtgccat ctagttcact gggaacccag acatatatct gcaacgtgaa tcacaagcca      720

agtaatacta aagtcgacaa gaaagtggaa cccaagtctt gtgataaaac tcatacctgc      780

ccaccctgtc ctgcaccaga ggctgcagga gggccatccg tgttcctgtt tcctccaaag      840

cccaaagaca ccctgatgat tagccggaca cccgaagtca cttgcgtggt cgtggacgtg      900

tcccacgagg accccgaagt caagtttaac tggtacgtgg atggcgtcga ggtgcataat      960

gccaagacaa aacccaggga ggaacagtac aacagtacct atagagtcgt gtcagtcctg     1020

acagtgctgc accaggactg gctgaacgga aaggagtata agtgcaaagt gtctaataag     1080

gctctgcccg cacctatcga gaaaaccatt agcaaggcca aagggcagcc ccgagaacct     1140

caggtgtaca cactgccccc ttcccgcgac gagctgacaa agaaccaggt ctctctgact     1200

tgtctggtga aaggattcta tccttcagat atcgccgtgg agtgggaaag caatgggcag     1260

ccagaaaaca attacaagac taccccaccc gtgctggact ctgatggcag tttctttctg     1320

tatagcaagc tgaccgtgga caaatcccgc tggcagcagg gaaacgtctt tagctgctcc     1380

gtgatgcatg aggccctgca caatcattac acccagaagt ctctgagtct gtcacctggg     1440

aagcgaggac gaaaaaggag aagcggctcc ggagctacaa acttctccct gctgaaacag     1500

gcaggagatg tggaggaaaa tccagggccc atggcctgga ctcctctgtt cctgtttctg     1560

ctgacctgct gtccaggcgg aagcaacagc tcctacgagg tgacccagcc tccaagcgtg     1620

agcgtgagcc caggccagac cgcttcaatc acatgtagcg gagacaagct ggggaagaaa     1680

tacactagtt ggtatcagca gaaaccaggg cagtcacccc tgctggtcat ctaccaggat     1740

accaagcgcc ctagcggcat tccagaacga ttcagcggct ccaactctgg aaatacagcc     1800

actctgacca tcagcggcac ccaggcaatg gacgaggccg attactattg ccaggcttgg     1860

gattccacaa ctcacgtcat tttcgggggc ggaaccaagc tgacagtgct gggacagccc     1920

aaagccgctc cttccgtcac cctgtttccc ccttctagtg aggaactgca ggccaataag     1980

gccaccctgg tgtgcctgat tagcgacttc taccccggag ctgtcactgt ggcatggaag     2040

gccgattcaa gccccgtcaa agcaggggtg gaaaccacaa ctccttcaaa gcagagcaac     2100

aacaagtacg cagcctcctc ttatctgtcc ctgacccctg agcagtggaa gtctcataaa     2160

agttattcat gtcaggtcac ccatgagggc agcacagtgg aaaaaaccgt ggcaccaaca     2220

gaatgtagct gataactcga g                                               2241


<210>  74
<211>  739
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DVSF-2 LALA amino acid sequence

<400>  74

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Trp Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Arg Tyr Asp Met His Trp Val Arg Gln Val Thr Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Ala Ile Thr Thr Ala Gly Asp Thr Tyr Tyr Pro Asp 
65                  70                  75                  80  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Lys Ser Ser 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Asn Leu Arg Ala Gly Asp Thr Ala Leu Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Gly Pro Pro Thr Asp Cys Ser Ser Gly Arg Cys Leu 
        115                 120                 125             


Gly Val Gly Val Gly Leu Asp Pro Trp Gly Gln Gly Thr Leu Val Thr 
    130                 135                 140                 


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
145                 150                 155                 160 


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
                165                 170                 175     


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
            180                 185                 190         


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
        195                 200                 205             


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
    210                 215                 220                 


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
225                 230                 235                 240 


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu 
            260                 265                 270         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
        275                 280                 285             


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
    290                 295                 300                 


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
305                 310                 315                 320 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
                325                 330                 335     


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
            340                 345                 350         


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
        355                 360                 365             


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
    370                 375                 380                 


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
385                 390                 395                 400 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
                405                 410                 415     


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
            420                 425                 430         


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
        435                 440                 445             


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
    450                 455                 460                 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg 
465                 470                 475                 480 


Lys Arg Arg Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln 
                485                 490                 495     


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala Trp Thr Pro Leu 
            500                 505                 510         


Phe Leu Phe Leu Leu Thr Cys Cys Pro Gly Gly Ser Asn Ser Ser Tyr 
        515                 520                 525             


Glu Val Thr Gln Pro Pro Ser Val Ser Val Ser Pro Gly Gln Thr Ala 
    530                 535                 540                 


Ser Ile Thr Cys Ser Gly Asp Lys Leu Gly Lys Lys Tyr Thr Ser Trp 
545                 550                 555                 560 


Tyr Gln Gln Lys Pro Gly Gln Ser Pro Leu Leu Val Ile Tyr Gln Asp 
                565                 570                 575     


Thr Lys Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser Asn Ser 
            580                 585                 590         


Gly Asn Thr Ala Thr Leu Thr Ile Ser Gly Thr Gln Ala Met Asp Glu 
        595                 600                 605             


Ala Asp Tyr Tyr Cys Gln Ala Trp Asp Ser Thr Thr His Val Ile Phe 
    610                 615                 620                 


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro 
625                 630                 635                 640 


Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn Lys 
                645                 650                 655     


Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val Thr 
            660                 665                 670         


Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu Thr 
        675                 680                 685             


Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr 
    690                 695                 700                 


Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Lys Ser Tyr Ser Cys 
705                 710                 715                 720 


Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro Thr 
                725                 730                 735     


Glu Cys Ser 
            


<210>  75
<211>  2229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DVSF-3 WT nucleic acid sequence

<400>  75
ggatccgcca ccatggactg gacatggaga atcctgttcc tggtcgccgc cgcaaccggg       60

acacacgccg aagtgcagct ggtggaatca gggggagggc tggtgcagcc tggaagaagt      120

ctgaggctgt catgcgccgc tagcggcttc acctttgacg attacgccat gttctgggtg      180

aggcaggctc caggcaaggg actggaatgg atcagcggca tttcctggaa ctctgcaact      240

atcgggtatg ccgactccgt gaaaggacgg tttaccattt caagagacaa cgccaagaaa      300

agcctggatc tgcagatgaa ttccctgcgg cccgacgata ccgctctgta ctattgcgca      360

aagggaggac ctagaggcct gcagctgctg agctcctggg tggactactg gggacagggc      420

actctggtca ccgtgtctag tgcttccaca aagggacctt ctgtgttccc actggcaccc      480

tcaagcaaat caacaagcgg aggaactgca gcactgggat gtctggtgaa ggattatttc      540

cccgagcctg tcaccgtgag ttggaactca ggagcactga cttccggagt ccacaccttt      600

ccagcagtgc tgcagtcctc tggactgtac agcctgagtt cagtggtcac agtgcctagc      660

tcctctctgg gcacacagac ttatatctgc aacgtgaatc acaagcctag caatactaaa      720

gtcgacaaga aagtggaacc aaagtcctgt gataaaaccc atacatgccc accttgtcca      780

gcaccagagc tgctgggggg accaagcgtg ttcctgtttc cacccaagcc caaagacaca      840

ctgatgattt ctcggacccc tgaagtcaca tgtgtggtcg tggacgtgag ccacgaggac      900

cccgaagtca agttcaactg gtacgtggat ggcgtcgagg tgcataatgc taagaccaaa      960

ccccgagagg aacagtacaa cagcacttat cgggtcgtgt ccgtcctgac cgtgctgcac     1020

caggactggc tgaacgggaa ggagtataag tgcaaagtgt ccaataaggc cctgcctgct     1080

ccaatcgaga aaacaatttc taaggcaaaa ggacagcctc gcgaaccaca ggtgtacact     1140

ctgcctccat cccgagacga gctgaccaag aaccaggtct ctctgacatg tctggtgaaa     1200

ggcttctatc caagtgatat cgctgtggag tgggaaagca atgggcagcc cgaaaacaat     1260

tacaagacca caccccctgt gctggacagc gatggctcct tctttctgta ttctaagctg     1320

accgtggata aaagtagatg gcagcagggg aacgtctttt cctgctctgt gatgcatgag     1380

gccctgcaca atcattacac acagaagagt ctgtcactga gcccagggaa gcgaggacgg     1440

aaacggagat ccgggtctgg agcaaccaac ttctccctgc tgaaacaggc aggcgacgtg     1500

gaggaaaatc caggacctat ggtcctgcag acccaggtgt ttatctctct gctgctgtgg     1560

attagtggcg cctacgggga tatccagatg acacagtccc ccagttcact gagtgcctca     1620

gtcggcgaca gggtgactat cacctgtcgc gctagccagg atattaggcg ctacctgaac     1680

tggtatcagc agcgaccagg acgagtgcct cagctgctga tctacactac ctccaccctg     1740

cagtctggag tcccaagtag gttcagcggc tccgggtctg tgacagactt tacactgact     1800

attagctccc tgcagcccga agatttcggc acttactatt gccagcagag ttattcacca     1860

ccccacacat ttggacaggg cactaagctg gaaatcaaaa ctgtcgctgc accctcagtg     1920

ttcatttttc ctccatctga cgagcagctg aagtcaggca ccgccagcgt cgtgtgtctg     1980

ctgaacaatt tctaccctcg cgaggctaag gtccagtgga aagtggataa cgcactgcag     2040

tctgggaata gtcaggagtc agtgacagaa caggacagca aggattccac ttattctctg     2100

tctagtaccc tgacactgag caaagccgac tacgagaagc acaaagtcta tgcttgcgaa     2160

gtgacccatc aggggctgag aagtcccgtg acaaagagct tcaacagggg agagtgttga     2220

taactcgag                                                             2229


<210>  76
<211>  735
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DVSF-3 WT amino acid sequence

<400>  76

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Asp Asp Tyr Ala Met Phe Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Ile Ser Gly Ile Ser Trp Asn Ser Ala Thr Ile Gly Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys 
                85                  90                  95      


Ser Leu Asp Leu Gln Met Asn Ser Leu Arg Pro Asp Asp Thr Ala Leu 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Gly Gly Pro Arg Gly Leu Gln Leu Leu Ser Ser 
        115                 120                 125             


Trp Val Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser 
465                 470                 475                 480 


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
                485                 490                 495     


Glu Glu Asn Pro Gly Pro Met Val Leu Gln Thr Gln Val Phe Ile Ser 
            500                 505                 510         


Leu Leu Leu Trp Ile Ser Gly Ala Tyr Gly Asp Ile Gln Met Thr Gln 
        515                 520                 525             


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
    530                 535                 540                 


Cys Arg Ala Ser Gln Asp Ile Arg Arg Tyr Leu Asn Trp Tyr Gln Gln 
545                 550                 555                 560 


Arg Pro Gly Arg Val Pro Gln Leu Leu Ile Tyr Thr Thr Ser Thr Leu 
                565                 570                 575     


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Val Thr Asp 
            580                 585                 590         


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Gly Thr Tyr 
        595                 600                 605             


Tyr Cys Gln Gln Ser Tyr Ser Pro Pro His Thr Phe Gly Gln Gly Thr 
    610                 615                 620                 


Lys Leu Glu Ile Lys Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro 
625                 630                 635                 640 


Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu 
                645                 650                 655     


Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp 
            660                 665                 670         


Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp 
        675                 680                 685             


Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys 
    690                 695                 700                 


Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln 
705                 710                 715                 720 


Gly Leu Arg Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
                725                 730                 735 


<210>  77
<211>  2229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DVSF-3 LALA nucleic acid sequence

<400>  77
ggatccgcca ccatggactg gacttggaga atcctgtttc tggtcgccgc cgcaactgga       60

acccacgccg aggtgcagct ggtcgaatca gggggaggcc tggtgcagcc tgggagaagt      120

ctgcggctgt catgcgccgc tagcggcttc acctttgacg attacgcaat gttctgggtg      180

aggcaggcac caggcaaggg actggaatgg atcagcggca tttcctggaa ctctgctacc      240

atcggatatg cagacagcgt gaaagggagg tttacaattt ctagagacaa cgccaagaaa      300

agtctggatc tgcagatgaa ttcactgcgc cccgacgata ccgccctgta ctattgcgct      360

aagggcggac ccaggggcct gcagctgctg agctcctggg tggactactg ggggcagggc      420

actctggtca ccgtgtctag tgcctccaca aagggcccta gcgtgttccc actggctccc      480

tcaagcaaat caacaagcgg gggcactgca gccctgggat gtctggtgaa ggattatttc      540

cccgagcctg tcaccgtgag ttggaactca ggggctctga ctagcggcgt ccacaccttt      600

cccgcagtgc tgcagtcctc tggcctgtac agcctgagtt cagtggtcac tgtccctagc      660

tcctctctgg gaacacagac ttatatctgc aacgtgaatc acaagccttc caataccaaa      720

gtcgacaaga aagtggaacc aaagtcttgt gataaaaccc atacatgccc tccctgtcca      780

gcaccagagg ctgcaggagg gccaagcgtg ttcctgtttc cacccaagcc caaagacaca      840

ctgatgatta gccggacccc tgaagtcaca tgcgtggtcg tggacgtgag ccacgaggac      900

cccgaagtca agtttaactg gtacgtggat ggcgtcgagg tgcataatgc taagaccaaa      960

ccccgagagg aacagtacaa cagtacttat agggtcgtgt cagtcctgac cgtgctgcac     1020

caggactggc tgaacgggaa ggagtataag tgcaaagtgt ccaataaggc actgcctgcc     1080

ccaatcgaga aaactatttc taaggctaaa ggccagccta gagaaccaca ggtgtacacc     1140

ctgcctccaa gccgggacga gctgaccaag aaccaggtca gcctgacatg tctggtgaaa     1200

ggattctatc catccgatat cgcagtggag tgggaatcta atgggcagcc cgaaaacaat     1260

tacaagacca caccccctgt gctggacagc gatggcagct tcttcctgta tagcaagctg     1320

accgtggata aatcccgctg gcagcagggg aacgtctttt cctgctctgt gatgcatgag     1380

gccctgcaca atcattacac acagaagagt ctgtcactga gcccaggaaa gcgagggagg     1440

aaaaggagat ccggatctgg ggctactaac ttctccctgc tgaagcaggc aggcgacgtg     1500

gaggaaaatc ccggacctat ggtcctgcag acacaggtgt ttatcagcct gctgctgtgg     1560

atttccggcg cttacggaga tatccagatg actcagtccc ccagttcact gagtgcatca     1620

gtcggcgacc gggtgactat cacctgtcgc gcctctcagg atattcggcg ctacctgaat     1680

tggtatcagc agcgaccagg acgagtgcct cagctgctga tctacactac ctccacactg     1740

cagtctggcg tcccaagtag gttcagcggc tccggatctg tgactgactt tacactgact     1800

attagctccc tgcagcccga ggatttcggc acctactatt gccagcagag ttattcacca     1860

ccccacacat ttgggcaggg cactaagctg gaaatcaaaa ccgtcgccgc tcccagcgtg     1920

ttcatctttc ctccaagtga cgagcagctg aagtcaggaa cagccagcgt ggtgtgcctg     1980

ctgaacaatt tctaccctag agaagccaag gtccagtgga aagtggataa cgctctgcag     2040

tctgggaata gtcaggagtc agtgacagaa caggacagca aggattccac ttattctctg     2100

tctagtaccc tgacactgag caaagcagac tacgagaagc ataaagtgta tgcctgcgaa     2160

gtcacccacc aggggctgcg gtcaccagtc acaaaatcct ttaacagagg cgaatgctga     2220

taactcgag                                                             2229


<210>  78
<211>  735
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DSVF-3 LALA amino acid sequence

<400>  78

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Asp Asp Tyr Ala Met Phe Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Ile Ser Gly Ile Ser Trp Asn Ser Ala Thr Ile Gly Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys 
                85                  90                  95      


Ser Leu Asp Leu Gln Met Asn Ser Leu Arg Pro Asp Asp Thr Ala Leu 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Gly Gly Pro Arg Gly Leu Gln Leu Leu Ser Ser 
        115                 120                 125             


Trp Val Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Arg Gly Arg Lys Arg Arg Ser 
465                 470                 475                 480 


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
                485                 490                 495     


Glu Glu Asn Pro Gly Pro Met Val Leu Gln Thr Gln Val Phe Ile Ser 
            500                 505                 510         


Leu Leu Leu Trp Ile Ser Gly Ala Tyr Gly Asp Ile Gln Met Thr Gln 
        515                 520                 525             


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
    530                 535                 540                 


Cys Arg Ala Ser Gln Asp Ile Arg Arg Tyr Leu Asn Trp Tyr Gln Gln 
545                 550                 555                 560 


Arg Pro Gly Arg Val Pro Gln Leu Leu Ile Tyr Thr Thr Ser Thr Leu 
                565                 570                 575     


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Val Thr Asp 
            580                 585                 590         


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Gly Thr Tyr 
        595                 600                 605             


Tyr Cys Gln Gln Ser Tyr Ser Pro Pro His Thr Phe Gly Gln Gly Thr 
    610                 615                 620                 


Lys Leu Glu Ile Lys Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro 
625                 630                 635                 640 


Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu 
                645                 650                 655     


Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp 
            660                 665                 670         


Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp 
        675                 680                 685             


Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys 
    690                 695                 700                 


Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln 
705                 710                 715                 720 


Gly Leu Arg Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
                725                 730                 735 


