                         SEQUENCE LISTING

<110>  NETRIS PHARMA
 
<120>  Novel anti-netrin-1 antibody

<130>  BET 14L3009

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  604
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SIGNAL
<222>  (1)..(24)

<220>
<221>  MISC_FEATURE
<222>  (339)..(381)
<223>  linear epitope

<400>  1

Met Met Arg Ala Val Trp Glu Ala Leu Ala Ala Leu Ala Ala Val Ala 
1               5                   10                  15      


Cys Leu Val Gly Ala Val Arg Gly Gly Pro Gly Leu Ser Met Phe Ala 
            20                  25                  30          


Gly Gln Ala Ala Gln Pro Asp Pro Cys Ser Asp Glu Asn Gly His Pro 
        35                  40                  45              


Arg Arg Cys Ile Pro Asp Phe Val Asn Ala Ala Phe Gly Lys Asp Val 
    50                  55                  60                  


Arg Val Ser Ser Thr Cys Gly Arg Pro Pro Ala Arg Tyr Cys Val Val 
65                  70                  75                  80  


Ser Glu Arg Gly Glu Glu Arg Leu Arg Ser Cys His Leu Cys Asn Ala 
                85                  90                  95      


Ser Asp Pro Lys Lys Ala His Pro Pro Ala Phe Leu Thr Asp Leu Asn 
            100                 105                 110         


Asn Pro His Asn Leu Thr Cys Trp Gln Ser Glu Asn Tyr Leu Gln Phe 
        115                 120                 125             


Pro His Asn Val Thr Leu Thr Leu Ser Leu Gly Lys Lys Phe Glu Val 
    130                 135                 140                 


Thr Tyr Val Ser Leu Gln Phe Cys Ser Pro Arg Pro Glu Ser Met Ala 
145                 150                 155                 160 


Ile Tyr Lys Ser Met Asp Tyr Gly Arg Thr Trp Val Pro Phe Gln Phe 
                165                 170                 175     


Tyr Ser Thr Gln Cys Arg Lys Met Tyr Asn Arg Pro His Arg Ala Pro 
            180                 185                 190         


Ile Thr Lys Gln Asn Glu Gln Glu Ala Val Cys Thr Asp Ser His Thr 
        195                 200                 205             


Asp Met Arg Pro Leu Ser Gly Gly Leu Ile Ala Phe Ser Thr Leu Asp 
    210                 215                 220                 


Gly Arg Pro Ser Ala His Asp Phe Asp Asn Ser Pro Val Leu Gln Asp 
225                 230                 235                 240 


Trp Val Thr Ala Thr Asp Ile Arg Val Ala Phe Ser Arg Leu His Thr 
                245                 250                 255     


Phe Gly Asp Glu Asn Glu Asp Asp Ser Glu Leu Ala Arg Asp Ser Tyr 
            260                 265                 270         


Phe Tyr Ala Val Ser Asp Leu Gln Val Gly Gly Arg Cys Lys Cys Asn 
        275                 280                 285             


Gly His Ala Ala Arg Cys Val Arg Asp Arg Asp Asp Ser Leu Val Cys 
    290                 295                 300                 


Asp Cys Arg His Asn Thr Ala Gly Pro Glu Cys Asp Arg Cys Lys Pro 
305                 310                 315                 320 


Phe His Tyr Asp Arg Pro Trp Gln Arg Ala Thr Ala Arg Glu Ala Asn 
                325                 330                 335     


Glu Cys Val Ala Cys Asn Cys Asn Leu His Ala Arg Arg Cys Arg Phe 
            340                 345                 350         


Asn Met Glu Leu Tyr Lys Leu Ser Gly Arg Lys Ser Gly Gly Val Cys 
        355                 360                 365             


Leu Asn Cys Arg His Asn Thr Ala Gly Arg His Cys His Tyr Cys Lys 
    370                 375                 380                 


Glu Gly Tyr Tyr Arg Asp Met Gly Lys Pro Ile Thr His Arg Lys Ala 
385                 390                 395                 400 


Cys Lys Ala Cys Asp Cys His Pro Val Gly Ala Ala Gly Lys Thr Cys 
                405                 410                 415     


Asn Gln Thr Thr Gly Gln Cys Pro Cys Lys Asp Gly Val Thr Gly Ile 
            420                 425                 430         


Thr Cys Asn Arg Cys Ala Lys Gly Tyr Gln Gln Ser Arg Ser Pro Ile 
        435                 440                 445             


Ala Pro Cys Ile Lys Ile Pro Val Ala Pro Pro Thr Thr Ala Ala Ser 
    450                 455                 460                 


Ser Val Glu Glu Pro Glu Asp Cys Asp Ser Tyr Cys Lys Ala Ser Lys 
465                 470                 475                 480 


Gly Lys Leu Lys Ile Asn Met Lys Lys Tyr Cys Lys Lys Asp Tyr Ala 
                485                 490                 495     


Val Gln Ile His Ile Leu Lys Ala Asp Lys Ala Gly Asp Trp Trp Lys 
            500                 505                 510         


Phe Thr Val Asn Ile Ile Ser Val Tyr Lys Gln Gly Thr Ser Arg Ile 
        515                 520                 525             


Arg Arg Gly Asp Gln Ser Leu Trp Ile Arg Ser Arg Asp Ile Ala Cys 
    530                 535                 540                 


Lys Cys Pro Lys Ile Lys Pro Leu Lys Lys Tyr Leu Leu Leu Gly Asn 
545                 550                 555                 560 


Ala Glu Asp Ser Pro Asp Gln Ser Gly Ile Val Ala Asp Lys Ser Ser 
                565                 570                 575     


Leu Val Ile Gln Trp Arg Asp Thr Trp Ala Arg Arg Leu Arg Lys Phe 
            580                 585                 590         


Gln Gln Arg Glu Lys Lys Gly Lys Cys Lys Lys Ala 
        595                 600                 


<210>  2
<211>  1817
<212>  DNA
<213>  Homo sapiens

<400>  2
atgatgcgcg cagtgtggga ggcgctggcg gcgctggcgg cggtggcgtg cctggtgggc       60

gcggtgcgcg gcgggcccgg gctcagcatg ttcgcgggcc aggcggcgca gcccgatccc      120

tgctcggacg agaacggcca cccgcgccgc tgcatcccgg actttgtcaa tgcggccttc      180

ggcaaggacg tgcgcgtgtc cagcacctgc ggccggcccc cggcgcgcta ctgcgtggtg      240

agcgagcgcg gcgaggagcg gctgcgctcg tgccacctct gcaacgcgtc cgaccccaag      300

aaggcgcacc cgcccgcctt cctcaccgac ctcaacaacc cgcacaacct gacgtgctgg      360

cagtccgaga actacctgca gttcccgcac aacgtcacgc tcacactgtc cctcggcaag      420

aagttcgaag tgacctacgt gagcctgcag ttctgctcgc cgcggcccga gtccatggcc      480

atctacaagt ccatggacta cgggcgcacg tgggtgccct tccagttcta ctccacgcag      540

tgccgcaaga tgtacaaccg gccgcaccgc gcgcccatca ccaagcagaa cgagcaggag      600

gccgtgtgca ccgactcgca caccgacatg cgcccgctct cgggcggcct catcgccttc      660

agcacgctgg acgggcggcc ctcggcgcac gacttcgaca actcgcccgt gctgcaggac      720

tgggtcacgg ccacagacat ccgcgtggcc ttcagccgcc tgcacacgtt cggcgacgag      780

aacgaggacg actcggagct ggcgcgcgac tcgtacttct acgcggtgtc cgacctgcag      840

gtgggcggcc ggtgcaagtg caacggccac gcggcccgct gcgtgcgcga ccgcgacgac      900

agcctggtgt gcgactgcag gcacaacacg gccggcccgg agtgcgaccg ctgcaagccc      960

ttccactacg accggccctg gcagcgcgcc acagcccgcg aagccaacga gtgcgtggcc     1020

tgtaactgca acctgcatgc ccggcgctgc cgcttcaaca tggagctcta caagctttcg     1080

gggcgcaaga gcggaggtgt ctgcctcaac tgtcgccaca acaccgccgg ccgccactgc     1140

cattactgca aggagggcta ctaccgcgac atgggcaagc ccatcaccca ccggaaggcc     1200

tgcaaagcct gtgattgcca ccctgtgggt gctgctggca aaacctgcaa ccaaaccacc     1260

ggccagtgtc cctgcaagga cggcgtgacg ggtatcacct gcaaccgctg cgccaaaggc     1320

taccagcaga gccgctctcc catcgccccc tgcataaaga tccctgtagc gccgccgacg     1380

actgcagcca gcagcgtgga ggagcctgaa gactgcgatt cctactgcaa ggcctccaag     1440

gggaagctga agattaacat gaaaaagtac tgcaagaagg actatgccgt ccagatccac     1500

atcctgaagg cggacaaggc gggggactgg tggaagttca cggtgaacat catctccgtg     1560

tataagcagg gcacgagccg catccgccgc ggtgaccaga gcctgtggat ccgctcgcgg     1620

gacatcgcct gcaagtgtcc caaaatcaag cccctcaaga agtacctgct gctgggcaac     1680

gcggaggact ctccggacca gagcggcatc gtggccgata aaagcagcct ggtgatccag     1740

tggcgggaca cgtgggcgcg gcggctgcgc aagttccagc agcgtgagaa gaagggcaag     1800

tgcaagaagg cctagcg                                                    1817


<210>  3
<211>  43
<212>  PRT
<213>  Homo sapiens

<400>  3

Val Ala Cys Asn Cys Asn Leu His Ala Arg Arg Cys Arg Phe Asn Met 
1               5                   10                  15      


Glu Leu Tyr Lys Leu Ser Gly Arg Lys Ser Gly Gly Val Cys Leu Asn 
            20                  25                  30          


Cys Arg His Asn Thr Ala Gly Arg His Cys His 
        35                  40              


<210>  4
<211>  129
<212>  DNA
<213>  Homo sapiens

<400>  4
gtggcctgta actgcaacct gcatgcccgg cgctgccgct tcaacatgga gctctacaag       60

ctttcggggc gcaagagcgg aggtgtctgc ctcaactgtc gccacaacac cgccggccgc      120

cactgccat                                                              129


<210>  5
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  5

Gly Tyr Thr Phe Thr Ser Tyr Asn 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  6

Ile Tyr Pro Gly Asn Gly Asp Thr 
1               5               


<210>  7
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  7

Ala Arg Gly Gly Thr Gly Phe Ala Tyr 
1               5                   


<210>  8
<211>  6
<212>  PRT
<213>  Mus musculus

<400>  8

Gln Ser Val Ser Asn Asp 
1               5       


<210>  9
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  9

Gln Gln Asp Tyr Ser Ser Pro Trp Thr 
1               5                   


<210>  10
<211>  116
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  10

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ala 
        115     


<210>  11
<211>  107
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  11

Ser Ile Val Met Thr Gln Thr Pro Lys Phe Leu Leu Val Ser Ala Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  12
<211>  440
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  12

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ala Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala 
        115                 120                 125             


Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly 
145                 150                 155                 160 


Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Glu Ser Asp 
                165                 170                 175     


Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg Pro 
            180                 185                 190         


Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile 
    210                 215                 220                 


Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro 
225                 230                 235                 240 


Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val 
                245                 250                 255     


Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val 
            260                 265                 270         


Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln 
        275                 280                 285             


Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln 
    290                 295                 300                 


Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala 
305                 310                 315                 320 


Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro 
                325                 330                 335     


Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala 
            340                 345                 350         


Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu 
        355                 360                 365             


Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr 
    370                 375                 380                 


Lys Asn Thr Gln Pro Ile Met Asn Thr Asn Gly Ser Tyr Phe Val Tyr 
385                 390                 395                 400 


Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe 
                405                 410                 415     


Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys 
            420                 425                 430         


Ser Leu Ser His Ser Pro Gly Lys 
        435                 440 


<210>  13
<211>  214
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  13

Ser Ile Val Met Thr Gln Thr Pro Lys Phe Leu Leu Val Ser Ala Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly 
        115                 120                 125             


Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile 
    130                 135                 140                 


Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu 
145                 150                 155                 160 


Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr 
            180                 185                 190         


Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser 
        195                 200                 205             


Phe Asn Arg Asn Glu Cys 
    210                 


<210>  14
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  14

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Ile Pro Pro Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Leu Thr Ile Asn Asn Ile Glu Ser 
65                  70                  75                  80  


Glu Asp Ala Ala Tyr Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly 
            100     


<210>  15
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  15

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Phe Gln Gln Arg Pro Gly Gln Ser Pro Arg Arg Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly 
            100     


<210>  16
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  16

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Ile Pro Pro Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Leu Thr Ile Asn Asn Ile Glu Ser 
65                  70                  75                  80  


Glu Asp Ala Ala Tyr Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly 
            100     


<210>  17
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  17

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly 
            100     


<210>  18
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  18

Asp Ile Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Lys Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Ile Pro Pro Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Leu Thr Ile Asn Asn Ile Glu Ser 
65                  70                  75                  80  


Glu Asp Ala Ala Tyr Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly 
            100     


<210>  19
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (27)..(32)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (50)..(52)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (89)..(97)
<223>  CDR according to IGMT

<400>  19

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Asn Asp 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Asp Tyr Ser Ser Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly 
            100     


<210>  20
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  20

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Thr Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 


<210>  21
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  21

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 


<210>  22
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  22

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Thr Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val 
65                  70                  75                  80  


Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 


<210>  23
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  23

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Thr Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 


<210>  24
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  24

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 


<210>  25
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  25

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Thr Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 


<210>  26
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  26

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Thr Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 


<210>  27
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized variant


<220>
<221>  MISC_FEATURE
<222>  (26)..(33)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (51)..(58)
<223>  CDR according to IGMT

<220>
<221>  MISC_FEATURE
<222>  (97)..(105)
<223>  CDR according to IGMT

<400>  27

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Thr Gly Phe Ala Tyr Trp Gly Gln 
            100                 105             


<210>  28
<211>  5
<212>  PRT
<213>  Mus musculus

<400>  28

Ser Tyr Asn Met His 
1               5   


<210>  29
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  29

Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  30
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  30

Gly Gly Thr Gly Phe Ala Tyr 
1               5           


<210>  31
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  31

Lys Ala Ser Gln Ser Val Ser Asn Asp Val Ala 
1               5                   10      


<210>  32
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  32

Tyr Ala Ser Asn Arg Tyr Thr 
1               5           


<210>  33
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for PCR of human netrin-1 gene

<400>  33
aaaagtactg caagaaggac tatgc                                             25


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for PCR of human netrin-1 gene

<400>  34
ccctgcttat acacggagat g                                                 21


<210>  35
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  35

Ala Arg Arg Cys Arg Phe Asn Met Glu Leu Tyr Lys Leu Ser Gly Arg 
1               5                   10                  15      


Lys Ser Gly Gly Val Cys 
            20          


<210>  36
<211>  66
<212>  DNA
<213>  cDNA

<400>  36
gcccggcgct gccgcttcaa catggagctc tacaagcttt cggggcgcaa gagcggaggt       60

gtctgc                                                                  66


