﻿               SEQUENCE LISTING

<110> SIRION BIOTECH GmbH
      Helmholtz Zentrum München

<120> Pseudotyped lentiviral vectors

<130> W1041 PCT

<150> EP14150846.5
<151> 2014-01-10

<160> 14

<170> BiSSAP 1.2

<210> 1
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..48
<223> /mol_type="unassigned DNA"
      /note="Endoplasmic reticulum (ER) signal sequence of VSV-G"
      /organism="Artificial Sequence"

<400> 1
atgaagtgcc ttttgtactt agccttttta ttcattgggg tgaattgc                  48


<210> 2
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Endoplasmic reticulum (ER) signal sequence of VSV-G

<400> 2
Met Lys Cys Leu Leu Tyr Leu Ala Phe Leu Phe Ile Gly Val Asn Cys 
1               5                   10                  15      



<210> 3
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..39
<223> /mol_type="unassigned DNA"
      /note="Linker between scFv and VSV-G"
      /organism="Artificial Sequence"

<400> 3
ggaggcggtt caggaggtgg ctcgagcgga ggcggttca                            39


<210> 4
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker between scFv and VSV-G

<400> 4
Gly Gly Gly Ser Gly Gly Gly Ser Ser Gly Gly Gly Ser 
1               5                   10              

<210> 5
<211> 1488
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1488
<223> /mol_type="unassigned DNA"
      /note="VSV-G without signal sequence"
      /organism="Artificial Sequence"

<400> 5
aagttcacca tagtttttcc acacaaccaa aaaggaaact ggaaaaatgt tccttctaat      60

taccattatt gcccgtcaag ctcagattta aattggcata atgacttaat aggcacagcc     120

atacaagtca aaatgcccaa gagtcacaag gctattcaag cagacggttg gatgtgtcat     180

gcttccaaat gggtcactac ttgtgatttc cgctggtatg gaccgaagta tataacacag     240

tccatccgat ccttcactcc atctgtagaa caatgcaagg aaagcattga acaaacgaaa     300

caaggaactt ggctgaatcc aggcttccct cctcaaagtt gtggatatgc aactgtgacg     360

gatgccgaag cagtgattgt ccaggtgact cctcaccatg tgctggttga tgaatacaca     420

ggagaatggg ttgattcaca gttcatcaac ggaaaatgca gcaattacat atgccccact     480

gtccataact ctacaacctg gcattctgac tataaggtca aagggctatg tgattctaac     540

ctcatttcca tggacatcac cttcttctca gaggacggag agctatcatc cctgggaaag     600

gagggcacag ggttcagaag taactacttt gcttatgaaa ctggaggcaa ggcctgcaaa     660

atgcaatact gcaagcattg gggagtcaga ctcccatcag gtgtctggtt cgagatggct     720

gataaggatc tctttgctgc agccagattc cctgaatgcc cagaagggtc aagtatctct     780

gctccatctc agacctcagt ggatgtaagt ctaattcagg acgttgagag gatcttggat     840

tattccctct gccaagaaac ctggagcaaa atcagagcgg gtcttccaat ctctccagtg     900

gatctcagct atcttgctcc taaaaaccca ggaaccggtc ctgctttcac cataatcaat     960

ggtaccctaa aatactttga gaccagatac atcagagtcg atattgctgc tccaatcctc    1020

tcaagaatgg tcggaatgat cagtggaact accacagaaa gggaactgtg ggatgactgg    1080

gcaccatatg aagacgtgga aattggaccc aatggagttc tgaggaccag ttcaggatat    1140

aagtttcctt tatacatgat tggacatggt atgttggact ccgatcttca tcttagctca    1200

aaggctcagg tgttcgaaca tcctcacatt caagacgctg cttcgcaact tcctgatgat    1260

gagagtttat tttttggtga tactgggcta tccaaaaatc caatcgagct tgtagaaggt    1320

tggttcagta gttggaaaag ctctattgcc tcttttttct ttatcatagg gttaatcatt    1380

ggactattct tggttctccg agttggtatc catctttgca ttaaattaaa gcacaccaag    1440

aaaagacaga tttatacaga catagagatg aaccgacttg gaaagtaa                 1488


<210> 6
<211> 495
<212> PRT
<213> Artificial Sequence

<220> 
<223> VSV-G without signal sequence

<400> 6
Lys Phe Thr Ile Val Phe Pro His Asn Gln Lys Gly Asn Trp Lys Asn 
1               5                   10                  15      
Val Pro Ser Asn Tyr His Tyr Cys Pro Ser Ser Ser Asp Leu Asn Trp 
            20                  25                  30          
His Asn Asp Leu Ile Gly Thr Ala Ile Gln Val Lys Met Pro Lys Ser 
        35                  40                  45              
His Lys Ala Ile Gln Ala Asp Gly Trp Met Cys His Ala Ser Lys Trp 
    50                  55                  60                  
Val Thr Thr Cys Asp Phe Arg Trp Tyr Gly Pro Lys Tyr Ile Thr Gln 
65                  70                  75                  80  
Ser Ile Arg Ser Phe Thr Pro Ser Val Glu Gln Cys Lys Glu Ser Ile 
                85                  90                  95      
Glu Gln Thr Lys Gln Gly Thr Trp Leu Asn Pro Gly Phe Pro Pro Gln 
            100                 105                 110         
Ser Cys Gly Tyr Ala Thr Val Thr Asp Ala Glu Ala Val Ile Val Gln 
        115                 120                 125             
Val Thr Pro His His Val Leu Val Asp Glu Tyr Thr Gly Glu Trp Val 
    130                 135                 140                 
Asp Ser Gln Phe Ile Asn Gly Lys Cys Ser Asn Tyr Ile Cys Pro Thr 
145                 150                 155                 160 
Val His Asn Ser Thr Thr Trp His Ser Asp Tyr Lys Val Lys Gly Leu 
                165                 170                 175     
Cys Asp Ser Asn Leu Ile Ser Met Asp Ile Thr Phe Phe Ser Glu Asp 
            180                 185                 190         
Gly Glu Leu Ser Ser Leu Gly Lys Glu Gly Thr Gly Phe Arg Ser Asn 
        195                 200                 205             
Tyr Phe Ala Tyr Glu Thr Gly Gly Lys Ala Cys Lys Met Gln Tyr Cys 
    210                 215                 220                 
Lys His Trp Gly Val Arg Leu Pro Ser Gly Val Trp Phe Glu Met Ala 
225                 230                 235                 240 
Asp Lys Asp Leu Phe Ala Ala Ala Arg Phe Pro Glu Cys Pro Glu Gly 
                245                 250                 255     
Ser Ser Ile Ser Ala Pro Ser Gln Thr Ser Val Asp Val Ser Leu Ile 
            260                 265                 270         
Gln Asp Val Glu Arg Ile Leu Asp Tyr Ser Leu Cys Gln Glu Thr Trp 
        275                 280                 285             
Ser Lys Ile Arg Ala Gly Leu Pro Ile Ser Pro Val Asp Leu Ser Tyr 
    290                 295                 300                 
Leu Ala Pro Lys Asn Pro Gly Thr Gly Pro Ala Phe Thr Ile Ile Asn 
305                 310                 315                 320 
Gly Thr Leu Lys Tyr Phe Glu Thr Arg Tyr Ile Arg Val Asp Ile Ala 
                325                 330                 335     
Ala Pro Ile Leu Ser Arg Met Val Gly Met Ile Ser Gly Thr Thr Thr 
            340                 345                 350         
Glu Arg Glu Leu Trp Asp Asp Trp Ala Pro Tyr Glu Asp Val Glu Ile 
        355                 360                 365             
Gly Pro Asn Gly Val Leu Arg Thr Ser Ser Gly Tyr Lys Phe Pro Leu 
    370                 375                 380                 
Tyr Met Ile Gly His Gly Met Leu Asp Ser Asp Leu His Leu Ser Ser 
385                 390                 395                 400 
Lys Ala Gln Val Phe Glu His Pro His Ile Gln Asp Ala Ala Ser Gln 
                405                 410                 415     
Leu Pro Asp Asp Glu Ser Leu Phe Phe Gly Asp Thr Gly Leu Ser Lys 
            420                 425                 430         
Asn Pro Ile Glu Leu Val Glu Gly Trp Phe Ser Ser Trp Lys Ser Ser 
        435                 440                 445             
Ile Ala Ser Phe Phe Phe Ile Ile Gly Leu Ile Ile Gly Leu Phe Leu 
    450                 455                 460                 
Val Leu Arg Val Gly Ile His Leu Cys Ile Lys Leu Lys His Thr Lys 
465                 470                 475                 480 
Lys Arg Gln Ile Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
                485                 490                 495 

<210> 7
<211> 2325
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2325
<223> /mol_type="unassigned DNA"
      /note="scFvCD30-VSV-G (Signal sequence of VSV-G - His-Tag -
      scFvCD30 - Linker - VSV-G)"
      /organism="Artificial Sequence"

<400> 7
atgaagtgcc ttttgtactt agccttttta ttcattgggg tcaattgcca tcatcatcat      60

catcatgccc aggtcaagct gcaggagtca gggactgaac tggcaaagcc tggggccgca     120

gtgaagatgt cctgcaaggc ttctggctac acctttactg actactggat gcactgggtt     180

aaacagaggc ctggacaggg tctggaatgg attggataca ttaatcctaa cactgcttat     240

actgactaca atcagaaatt caaggacaag gccacattga ctgcagacaa atcctccagc     300

acagcctaca tgcaactgcg cagcctgacc tctgaggatt ctgcagtcta ttactgtgca     360

aaaaagacaa ctcagactac gtgggggttt cctttttggg gccaagggac cacggtcacc     420

gtctcctcag gtggaggcgg ttcaggcgga ggtggctctg gcggtggcgg atcggacatt     480

gtgctgaccc agtctccaaa atccatggcc atgtcagtcg gagagagggt caccttgagc     540

tgcaaggcca gtgagaatgt ggattctttt gtttcctggt atcaacagaa accaggccag     600

tctcctaaac tgctgatata cggggcctcc aaccggtaca ctggggtccc cgatcgcttc     660

gcaggcagtg gatctggaag agatttcact ctgaccatca gcagtgtgca ggctgaagac     720

cttgcagatt atcactgtgg acagaattac aggtatccgc tcacgttcgg tgctggcacc     780

aagctggaaa tcaaacgggg aggcggttca ggaggtggct cgagcggagg cggttcaaag     840

ttcaccatag tttttccaca caaccaaaaa ggaaactgga aaaatgttcc ttctaattac     900

cattattgcc cgtcaagctc agatttaaat tggcataatg acttaatagg cacagccata     960

caagtcaaaa tgcccaagag tcacaaggct attcaagcag acggttggat gtgtcatgct    1020

tccaaatggg tcactacttg tgatttccgc tggtatggac cgaagtatat aacacagtcc    1080

atccgatcct tcactccatc tgtagaacaa tgcaaggaaa gcattgaaca aacgaaacaa    1140

ggaacttggc tgaatccagg cttccctcct caaagttgtg gatatgcaac tgtgacggat    1200

gccgaagcag tgattgtcca ggtgactcct caccatgtgc tggttgatga atacacagga    1260

gaatgggttg attcacagtt catcaacgga aaatgcagca attacatatg ccccactgtc    1320

cataactcta caacctggca ttctgactat aaggtcaaag ggctatgtga ttctaacctc    1380

atttccatgg acatcacctt cttctcagag gacggagagc tatcatccct gggaaaggag    1440

ggcacagggt tcagaagtaa ctactttgct tatgaaactg gaggcaaggc ctgcaaaatg    1500

caatactgca agcattgggg agtcagactc ccatcaggtg tctggttcga gatggctgat    1560

aaggatctct ttgctgcagc cagattccct gaatgcccag aagggtcaag tatctctgct    1620

ccatctcaga cctcagtgga tgtaagtcta attcaggacg ttgagaggat cttggattat    1680

tccctctgcc aagaaacctg gagcaaaatc agagcgggtc ttccaatctc tccagtggat    1740

ctcagctatc ttgctcctaa aaacccagga accggtcctg ctttcaccat aatcaatggt    1800

accctaaaat actttgagac cagatacatc agagtcgata ttgctgctcc aatcctctca    1860

agaatggtcg gaatgatcag tggaactacc acagaaaggg aactgtggga tgactgggca    1920

ccatatgaag acgtggaaat tggacccaat ggagttctga ggaccagttc aggatataag    1980

tttcctttat acatgattgg acatggtatg ttggactccg atcttcatct tagctcaaag    2040

gctcaggtgt tcgaacatcc tcacattcaa gacgctgctt cgcaacttcc tgatgatgag    2100

agtttatttt ttggtgatac tgggctatcc aaaaatccaa tcgagcttgt agaaggttgg    2160

ttcagtagtt ggaaaagctc tattgcctct tttttcttta tcatagggtt aatcattgga    2220

ctattcttgg ttctccgagt tggtatccat ctttgcatta aattaaagca caccaagaaa    2280

agacagattt atacagacat agagatgaac cgacttggaa agtaa                    2325


<210> 8
<211> 774
<212> PRT
<213> Artificial Sequence

<220> 
<223> scFvCD30-VSV-G (Signal sequence of VSV-G - His-Tag - scFvCD30 -
      Linker - VSV-G)

<400> 8
Met Lys Cys Leu Leu Tyr Leu Ala Phe Leu Phe Ile Gly Val Asn Cys 
1               5                   10                  15      
His His His His His His Ala Gln Val Lys Leu Gln Glu Ser Gly Thr 
            20                  25                  30          
Glu Leu Ala Lys Pro Gly Ala Ala Val Lys Met Ser Cys Lys Ala Ser 
        35                  40                  45              
Gly Tyr Thr Phe Thr Asp Tyr Trp Met His Trp Val Lys Gln Arg Pro 
    50                  55                  60                  
Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Asn Thr Ala Tyr 
65                  70                  75                  80  
Thr Asp Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp 
                85                  90                  95      
Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Arg Ser Leu Thr Ser Glu 
            100                 105                 110         
Asp Ser Ala Val Tyr Tyr Cys Ala Lys Lys Thr Thr Gln Thr Thr Trp 
        115                 120                 125             
Gly Phe Pro Phe Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 
Val Leu Thr Gln Ser Pro Lys Ser Met Ala Met Ser Val Gly Glu Arg 
                165                 170                 175     
Val Thr Leu Ser Cys Lys Ala Ser Glu Asn Val Asp Ser Phe Val Ser 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Gly 
        195                 200                 205             
Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Ala Gly Ser Gly 
    210                 215                 220                 
Ser Gly Arg Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp 
225                 230                 235                 240 
Leu Ala Asp Tyr His Cys Gly Gln Asn Tyr Arg Tyr Pro Leu Thr Phe 
                245                 250                 255     
Gly Ala Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Ser Gly Gly 
            260                 265                 270         
Gly Ser Ser Gly Gly Gly Ser Lys Phe Thr Ile Val Phe Pro His Asn 
        275                 280                 285             
Gln Lys Gly Asn Trp Lys Asn Val Pro Ser Asn Tyr His Tyr Cys Pro 
    290                 295                 300                 
Ser Ser Ser Asp Leu Asn Trp His Asn Asp Leu Ile Gly Thr Ala Ile 
305                 310                 315                 320 
Gln Val Lys Met Pro Lys Ser His Lys Ala Ile Gln Ala Asp Gly Trp 
                325                 330                 335     
Met Cys His Ala Ser Lys Trp Val Thr Thr Cys Asp Phe Arg Trp Tyr 
            340                 345                 350         
Gly Pro Lys Tyr Ile Thr Gln Ser Ile Arg Ser Phe Thr Pro Ser Val 
        355                 360                 365             
Glu Gln Cys Lys Glu Ser Ile Glu Gln Thr Lys Gln Gly Thr Trp Leu 
    370                 375                 380                 
Asn Pro Gly Phe Pro Pro Gln Ser Cys Gly Tyr Ala Thr Val Thr Asp 
385                 390                 395                 400 
Ala Glu Ala Val Ile Val Gln Val Thr Pro His His Val Leu Val Asp 
                405                 410                 415     
Glu Tyr Thr Gly Glu Trp Val Asp Ser Gln Phe Ile Asn Gly Lys Cys 
            420                 425                 430         
Ser Asn Tyr Ile Cys Pro Thr Val His Asn Ser Thr Thr Trp His Ser 
        435                 440                 445             
Asp Tyr Lys Val Lys Gly Leu Cys Asp Ser Asn Leu Ile Ser Met Asp 
    450                 455                 460                 
Ile Thr Phe Phe Ser Glu Asp Gly Glu Leu Ser Ser Leu Gly Lys Glu 
465                 470                 475                 480 
Gly Thr Gly Phe Arg Ser Asn Tyr Phe Ala Tyr Glu Thr Gly Gly Lys 
                485                 490                 495     
Ala Cys Lys Met Gln Tyr Cys Lys His Trp Gly Val Arg Leu Pro Ser 
            500                 505                 510         
Gly Val Trp Phe Glu Met Ala Asp Lys Asp Leu Phe Ala Ala Ala Arg 
        515                 520                 525             
Phe Pro Glu Cys Pro Glu Gly Ser Ser Ile Ser Ala Pro Ser Gln Thr 
    530                 535                 540                 
Ser Val Asp Val Ser Leu Ile Gln Asp Val Glu Arg Ile Leu Asp Tyr 
545                 550                 555                 560 
Ser Leu Cys Gln Glu Thr Trp Ser Lys Ile Arg Ala Gly Leu Pro Ile 
                565                 570                 575     
Ser Pro Val Asp Leu Ser Tyr Leu Ala Pro Lys Asn Pro Gly Thr Gly 
            580                 585                 590         
Pro Ala Phe Thr Ile Ile Asn Gly Thr Leu Lys Tyr Phe Glu Thr Arg 
        595                 600                 605             
Tyr Ile Arg Val Asp Ile Ala Ala Pro Ile Leu Ser Arg Met Val Gly 
    610                 615                 620                 
Met Ile Ser Gly Thr Thr Thr Glu Arg Glu Leu Trp Asp Asp Trp Ala 
625                 630                 635                 640 
Pro Tyr Glu Asp Val Glu Ile Gly Pro Asn Gly Val Leu Arg Thr Ser 
                645                 650                 655     
Ser Gly Tyr Lys Phe Pro Leu Tyr Met Ile Gly His Gly Met Leu Asp 
            660                 665                 670         
Ser Asp Leu His Leu Ser Ser Lys Ala Gln Val Phe Glu His Pro His 
        675                 680                 685             
Ile Gln Asp Ala Ala Ser Gln Leu Pro Asp Asp Glu Ser Leu Phe Phe 
    690                 695                 700                 
Gly Asp Thr Gly Leu Ser Lys Asn Pro Ile Glu Leu Val Glu Gly Trp 
705                 710                 715                 720 
Phe Ser Ser Trp Lys Ser Ser Ile Ala Ser Phe Phe Phe Ile Ile Gly 
                725                 730                 735     
Leu Ile Ile Gly Leu Phe Leu Val Leu Arg Val Gly Ile His Leu Cys 
            740                 745                 750         
Ile Lys Leu Lys His Thr Lys Lys Arg Gln Ile Tyr Thr Asp Ile Glu 
        755                 760                 765             
Met Asn Arg Leu Gly Lys 
    770                 

<210> 9
<211> 2344
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2344
<223> /mol_type="unassigned DNA"
      /note="scFvEGFR-VSV-G (Signal sequence of VSV-G - His-Tag -
      scFvEGFR - Linker - VSV-G)"
      /organism="Artificial Sequence"

<400> 9
atgaagtgcc ttttgtactt agccttttta ttcattgggg tcaattgcca tcatcatcat      60

catcatgccg aggtgcaact gcagcagtct ggggctgaac tggtgaagcc tggggcttca     120

gtgaagttgt cctgcaaggc ttccggctac accttcacca gccactggat gcactgggtg     180

aagcagaggg ctggacaagg ccttgagtgg atcggagagt ttaatcccag caacggccgt     240

actaactaca atgagaaatt caagagcaag gccacactga ctgtagacaa atcctccagc     300

acagcctaca tgcaactcag cagcctgaca tctgaggact ctgcggtcta ttactgtgcc     360

agtcgggact atgattacga cggacggtac tttgactact ggggccaagg gaccacggtc     420

accgtctcct caggtggcgg tggctcgggc ggtggtgggt cgggtggtgg cggatctgac     480

atcgagctca cccagtctcc agcaatcatg tctgcatctc caggggagaa ggtcactatg     540

acctgcagtg ccagctcaag tgtaacttac atgtattggt accagcagaa gccaggatcc     600

tcccccagac tcctgattta tgacacatcc aacctggctt ctggagtccc tgttcgtttc     660

agtggcagtg ggtctgggac ctcttactct ctcacaatca gccgaatgga ggctgaagat     720

gctgccactt attactgcca gcagtggagt agtcacatat tcacgttcgg ctcggggaca     780

gaactcgaga tcaaacgggt cgagcggagg cggttcagga ggcggttcag gaggtggctc     840

gagcggaggc ggttcaaagt tcaccatagt ttttccacac aaccaaaaag gaaactggaa     900

aaatgttcct tctaattacc attattgccc gtcaagctca gatttaaatt ggcataatga     960

cttaataggc acagccatac aagtcaaaat gcccaagagt cacaaggcta ttcaagcaga    1020

cggttggatg tgtcatgctt ccaaatgggt cactacttgt gatttccgct ggtatggacc    1080

gaagtatata acacagtcca tccgatcctt cactccatct gtagaacaat gcaaggaaag    1140

cattgaacaa acgaaacaag gaacttggct gaatccaggc ttccctcctc aaagttgtgg    1200

atatgcaact gtgacggatg ccgaagcagt gattgtccag gtgactcctc accatgtgct    1260

ggttgatgaa tacacaggag aatgggttga ttcacagttc atcaacggaa aatgcagcaa    1320

ttacatatgc cccactgtcc ataactctac aacctggcat tctgactata aggtcaaagg    1380

gctatgtgat tctaacctca tttccatgga catcaccttc ttctcagagg acggagagct    1440

atcatccctg ggaaaggagg gcacagggtt cagaagtaac tactttgctt atgaaactgg    1500

aggcaaggcc tgcaaaatgc aatactgcaa gcattgggga gtcagactcc catcaggtgt    1560

ctggttcgag atggctgata aggatctctt tgctgcagcc agattccctg aatgcccaga    1620

agggtcaagt atctctgctc catctcagac ctcagtggat gtaagtctaa ttcaggacgt    1680

tgagaggatc ttggattatt ccctctgcca agaaacctgg agcaaaatca gagcgggtct    1740

tccaatctct ccagtggatc tcagctatct tgctcctaaa aacccaggaa ccggtcctgc    1800

tttcaccata atcaatggta ccctaaaata ctttgagacc agatacatca gagtcgatat    1860

tgctgctcca atcctctcaa gaatggtcgg aatgatcagt ggaactacca cagaaaggga    1920

actgtgggat gactgggcac catatgaaga cgtggaaatt ggacccaatg gagttctgag    1980

gaccagttca ggatataagt ttcctttata catgattgga catggtatgt tggactccga    2040

tcttcatctt agctcaaagg ctcaggtgtt cgaacatcct cacattcaag acgctgcttc    2100

gcaacttcct gatgatgaga gtttattttt tggtgatact gggctatcca aaaatccaat    2160

cgagcttgta gaaggttggt tcagtagttg gaaaagctct attgcctctt ttttctttat    2220

catagggtta atcattggac tattcttggt tctccgagtt ggtatccatc tttgcattaa    2280

attaaagcac accaagaaaa gacagattta tacagacata gagatgaacc gacttggaaa    2340

gtaa                                                                 2344


<210> 10
<211> 780
<212> PRT
<213> Artificial Sequence

<220> 
<223> scFvEGFR-VSV-G (Signal sequence of VSV-G - His-Tag - scFvEGFR -
      Linker - VSV-G)

<400> 10
Met Lys Cys Leu Leu Tyr Leu Ala Phe Leu Phe Ile Gly Val Asn Cys 
1               5                   10                  15      
His His His His His His Ala Glu Val Gln Leu Gln Gln Ser Gly Ala 
            20                  25                  30          
Glu Leu Val Lys Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser 
        35                  40                  45              
Gly Tyr Thr Phe Thr Ser His Trp Met His Trp Val Lys Gln Arg Ala 
    50                  55                  60                  
Gly Gln Gly Leu Glu Trp Ile Gly Glu Phe Asn Pro Ser Asn Gly Arg 
65                  70                  75                  80  
Thr Asn Tyr Asn Glu Lys Phe Lys Ser Lys Ala Thr Leu Thr Val Asp 
                85                  90                  95      
Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu 
            100                 105                 110         
Asp Ser Ala Val Tyr Tyr Cys Ala Ser Arg Asp Tyr Asp Tyr Asp Gly 
        115                 120                 125             
Arg Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 
Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu 
                165                 170                 175     
Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Thr Tyr Met Tyr 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Arg Leu Leu Ile Tyr Asp 
        195                 200                 205             
Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 
Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu Asp 
225                 230                 235                 240 
Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser His Ile Phe Thr Phe 
                245                 250                 255     
Gly Ser Gly Thr Glu Leu Glu Ile Lys Arg Val Glu Arg Arg Arg Phe 
            260                 265                 270         
Gly Gly Gly Ser Gly Gly Gly Ser Ser Gly Gly Gly Ser Lys Phe Thr 
        275                 280                 285             
Ile Val Phe Pro His Asn Gln Lys Gly Asn Trp Lys Asn Val Pro Ser 
    290                 295                 300                 
Asn Tyr His Tyr Cys Pro Ser Ser Ser Asp Leu Asn Trp His Asn Asp 
305                 310                 315                 320 
Leu Ile Gly Thr Ala Ile Gln Val Lys Met Pro Lys Ser His Lys Ala 
                325                 330                 335     
Ile Gln Ala Asp Gly Trp Met Cys His Ala Ser Lys Trp Val Thr Thr 
            340                 345                 350         
Cys Asp Phe Arg Trp Tyr Gly Pro Lys Tyr Ile Thr Gln Ser Ile Arg 
        355                 360                 365             
Ser Phe Thr Pro Ser Val Glu Gln Cys Lys Glu Ser Ile Glu Gln Thr 
    370                 375                 380                 
Lys Gln Gly Thr Trp Leu Asn Pro Gly Phe Pro Pro Gln Ser Cys Gly 
385                 390                 395                 400 
Tyr Ala Thr Val Thr Asp Ala Glu Ala Val Ile Val Gln Val Thr Pro 
                405                 410                 415     
His His Val Leu Val Asp Glu Tyr Thr Gly Glu Trp Val Asp Ser Gln 
            420                 425                 430         
Phe Ile Asn Gly Lys Cys Ser Asn Tyr Ile Cys Pro Thr Val His Asn 
        435                 440                 445             
Ser Thr Thr Trp His Ser Asp Tyr Lys Val Lys Gly Leu Cys Asp Ser 
    450                 455                 460                 
Asn Leu Ile Ser Met Asp Ile Thr Phe Phe Ser Glu Asp Gly Glu Leu 
465                 470                 475                 480 
Ser Ser Leu Gly Lys Glu Gly Thr Gly Phe Arg Ser Asn Tyr Phe Ala 
                485                 490                 495     
Tyr Glu Thr Gly Gly Lys Ala Cys Lys Met Gln Tyr Cys Lys His Trp 
            500                 505                 510         
Gly Val Arg Leu Pro Ser Gly Val Trp Phe Glu Met Ala Asp Lys Asp 
        515                 520                 525             
Leu Phe Ala Ala Ala Arg Phe Pro Glu Cys Pro Glu Gly Ser Ser Ile 
    530                 535                 540                 
Ser Ala Pro Ser Gln Thr Ser Val Asp Val Ser Leu Ile Gln Asp Val 
545                 550                 555                 560 
Glu Arg Ile Leu Asp Tyr Ser Leu Cys Gln Glu Thr Trp Ser Lys Ile 
                565                 570                 575     
Arg Ala Gly Leu Pro Ile Ser Pro Val Asp Leu Ser Tyr Leu Ala Pro 
            580                 585                 590         
Lys Asn Pro Gly Thr Gly Pro Ala Phe Thr Ile Ile Asn Gly Thr Leu 
        595                 600                 605             
Lys Tyr Phe Glu Thr Arg Tyr Ile Arg Val Asp Ile Ala Ala Pro Ile 
    610                 615                 620                 
Leu Ser Arg Met Val Gly Met Ile Ser Gly Thr Thr Thr Glu Arg Glu 
625                 630                 635                 640 
Leu Trp Asp Asp Trp Ala Pro Tyr Glu Asp Val Glu Ile Gly Pro Asn 
                645                 650                 655     
Gly Val Leu Arg Thr Ser Ser Gly Tyr Lys Phe Pro Leu Tyr Met Ile 
            660                 665                 670         
Gly His Gly Met Leu Asp Ser Asp Leu His Leu Ser Ser Lys Ala Gln 
        675                 680                 685             
Val Phe Glu His Pro His Ile Gln Asp Ala Ala Ser Gln Leu Pro Asp 
    690                 695                 700                 
Asp Glu Ser Leu Phe Phe Gly Asp Thr Gly Leu Ser Lys Asn Pro Ile 
705                 710                 715                 720 
Glu Leu Val Glu Gly Trp Phe Ser Ser Trp Lys Ser Ser Ile Ala Ser 
                725                 730                 735     
Phe Phe Phe Ile Ile Gly Leu Ile Ile Gly Leu Phe Leu Val Leu Arg 
            740                 745                 750         
Val Gly Ile His Leu Cys Ile Lys Leu Lys His Thr Lys Lys Arg Gln 
        755                 760                 765             
Ile Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
    770                 775                 780 

<210> 11
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..69
<223> /mol_type="unassigned DNA"
      /note="MfeI-His-tag sense primer"
      /organism="Artificial Sequence"

<400> 11
gcgaccaatt gccatcatca tcatcatcat gcccaggtca agctgcagga gtggactgaa     60

ctggcaaag                                                             69


<210> 12
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..70
<223> /mol_type="unassigned DNA"
      /note="antisense primer, including half a linker sequence and
      harbouring an XhoI site "
      /organism="Artificial Sequence"

<400> 12
gtaatctcga gccacctcct gaaccgcctc cccgtttgat ttccagcttg gtgccacacc     60

gaacgtggcg                                                            70


<210> 13
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..62
<223> /mol_type="unassigned DNA"
      /note="sense primer, including half a linker sequence"
      /organism="Artificial Sequence"

<400> 13
gttatctcga gcggaggcgg ttcaaagttc accatagttt ttccacacaa caaaagaaac     60

tg                                                                    62


<210> 14
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..49
<223> /mol_type="unassigned DNA"
      /note="antisense primer"
      /organism="Artificial Sequence"

<400> 14
gtattaccgg ttcctgggtt tttaggagca agatagctga gatccactg                 49


