                         SEQUENCE LISTING

<110>  Whitehead Institute for Biomedical Research
       Massachusetts Institute of Technology
 
<120>  METABOLIC FLUX BIOSENSOR

<130>  W0571.70043WO00

<150>  US 61/925176
<151>  2014-01-08

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  619
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Val Lys Glu Ser Ile Ile Ala Leu Ala Glu His Ala Ala Ser Arg 
1               5                   10                  15      


Ala Ser Arg Val Ile Pro Pro Val Lys Leu Ala Tyr Lys Asn Met Leu 
            20                  25                  30          


Lys Asp Pro Ser Ser Lys Tyr Lys Pro Phe Asn Ala Pro Lys Leu Ser 
        35                  40                  45              


Asn Arg Lys Trp Pro Asp Asn Arg Ile Thr Arg Ala Pro Arg Trp Leu 
    50                  55                  60                  


Ser Thr Asp Leu Arg Asp Gly Asn Gln Ser Leu Pro Asp Pro Met Ser 
65                  70                  75                  80  


Val Glu Gln Lys Lys Glu Tyr Phe His Lys Leu Val Asn Ile Gly Phe 
                85                  90                  95      


Lys Glu Ile Glu Val Ser Phe Pro Ser Ala Ser Gln Thr Asp Phe Asp 
            100                 105                 110         


Phe Thr Arg Tyr Ala Val Glu Asn Ala Pro Asp Asp Val Ser Ile Gln 
        115                 120                 125             


Cys Leu Val Gln Ser Arg Glu His Leu Ile Lys Arg Thr Val Glu Ala 
    130                 135                 140                 


Leu Thr Gly Ala Lys Lys Ala Thr Ile His Thr Tyr Leu Ala Thr Ser 
145                 150                 155                 160 


Asp Met Phe Arg Glu Ile Val Phe Asn Met Ser Arg Glu Glu Ala Ile 
                165                 170                 175     


Ser Lys Ala Val Glu Ala Thr Lys Leu Val Arg Lys Leu Thr Lys Asp 
            180                 185                 190         


Asp Pro Ser Gln Gln Ala Thr Arg Trp Ser Tyr Glu Phe Ser Pro Glu 
        195                 200                 205             


Cys Phe Ser Asp Thr Pro Gly Glu Phe Ala Val Glu Ile Cys Glu Ala 
    210                 215                 220                 


Val Lys Lys Ala Trp Glu Pro Thr Glu Glu Asn Pro Ile Ile Phe Asn 
225                 230                 235                 240 


Leu Pro Ala Thr Val Glu Val Ala Ser Pro Asn Val Tyr Ala Asp Gln 
                245                 250                 255     


Ile Glu Tyr Phe Ala Thr His Ile Thr Glu Arg Glu Lys Val Cys Ile 
            260                 265                 270         


Ser Thr His Cys His Asn Asp Arg Gly Cys Gly Val Ala Ala Thr Glu 
        275                 280                 285             


Leu Gly Met Leu Ala Gly Ala Asp Arg Val Glu Gly Cys Leu Phe Gly 
    290                 295                 300                 


Asn Gly Glu Arg Thr Gly Asn Val Asp Leu Val Thr Val Ala Met Asn 
305                 310                 315                 320 


Met Tyr Thr Gln Gly Val Ser Pro Asn Leu Asp Phe Ser Asp Leu Thr 
                325                 330                 335     


Ser Val Leu Asp Val Val Glu Arg Cys Asn Lys Ile Pro Val Ser Gln 
            340                 345                 350         


Arg Ala Pro Tyr Gly Gly Asp Leu Val Val Cys Ala Phe Ser Gly Ser 
        355                 360                 365             


His Gln Asp Ala Ile Lys Lys Gly Phe Asn Leu Gln Asn Lys Lys Arg 
    370                 375                 380                 


Ala Gln Gly Glu Thr Gln Trp Arg Ile Pro Tyr Leu Pro Leu Asp Pro 
385                 390                 395                 400 


Lys Asp Ile Gly Arg Asp Tyr Glu Ala Val Ile Arg Val Asn Ser Gln 
                405                 410                 415     


Ser Gly Lys Gly Gly Ala Ala Trp Val Ile Leu Arg Ser Leu Gly Leu 
            420                 425                 430         


Asp Leu Pro Arg Asn Met Gln Ile Glu Phe Ser Ser Ala Val Gln Asp 
        435                 440                 445             


His Ala Asp Ser Leu Gly Arg Glu Leu Lys Ser Asp Glu Ile Ser Lys 
    450                 455                 460                 


Leu Phe Lys Glu Ala Tyr Asn Tyr Asn Asp Glu Gln Tyr Gln Ala Ile 
465                 470                 475                 480 


Ser Leu Val Asn Tyr Asn Val Glu Lys Phe Gly Thr Glu Arg Arg Val 
                485                 490                 495     


Phe Thr Gly Gln Val Lys Val Gly Asp Gln Ile Val Asp Ile Glu Gly 
            500                 505                 510         


Thr Gly Asn Gly Pro Ile Ser Ser Leu Val Asp Ala Leu Ser Asn Leu 
        515                 520                 525             


Leu Asn Val Arg Phe Ala Val Ala Asn Tyr Thr Glu His Ser Leu Gly 
    530                 535                 540                 


Ser Gly Ser Ser Thr Gln Ala Ala Ser Tyr Ile His Leu Ser Tyr Arg 
545                 550                 555                 560 


Arg Asn Ala Asp Asn Glu Lys Ala Tyr Lys Trp Gly Val Gly Val Ser 
                565                 570                 575     


Glu Asp Val Gly Asp Ser Ser Val Arg Ala Ile Phe Ala Thr Ile Asn 
            580                 585                 590         


Asn Ile Ile His Ser Gly Asp Val Ser Ile Pro Ser Leu Ala Glu Val 
        595                 600                 605             


Glu Gly Lys Asn Ala Ala Ala Ser Gly Ser Ala 
    610                 615                 


<210>  2
<211>  410
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  2

Met Val Lys Glu Ser Ile Ile Ala Leu Ala Glu His Ala Ala Ser Arg 
1               5                   10                  15      


Ala Ser Arg Val Ile Pro Pro Val Lys Leu Ala Tyr Lys Asn Met Leu 
            20                  25                  30          


Lys Asp Pro Ser Ser Lys Tyr Lys Pro Phe Asn Ala Pro Lys Leu Ser 
        35                  40                  45              


Asn Arg Lys Trp Pro Asp Asn Arg Ile Thr Arg Ala Pro Arg Trp Leu 
    50                  55                  60                  


Ser Thr Asp Leu Arg Asp Gly Asn Gln Ser Leu Pro Asp Pro Met Ser 
65                  70                  75                  80  


Val Glu Gln Lys Lys Glu Tyr Phe His Lys Leu Val Asn Ile Gly Phe 
                85                  90                  95      


Lys Glu Ile Glu Val Ser Phe Pro Ser Ala Ser Gln Thr Asp Phe Asp 
            100                 105                 110         


Phe Thr Arg Tyr Ala Val Glu Asn Ala Pro Asp Asp Val Ser Ile Gln 
        115                 120                 125             


Cys Leu Val Gln Ser Arg Glu His Leu Ile Lys Arg Thr Val Glu Ala 
    130                 135                 140                 


Leu Thr Gly Ala Lys Lys Ala Thr Ile His Thr Tyr Leu Ala Thr Ser 
145                 150                 155                 160 


Asp Met Phe Arg Glu Ile Val Phe Asn Met Ser Arg Glu Glu Ala Ile 
                165                 170                 175     


Ser Lys Ala Val Glu Ala Thr Lys Leu Val Arg Lys Leu Thr Lys Asp 
            180                 185                 190         


Asp Pro Ser Gln Gln Ala Thr Arg Trp Ser Tyr Glu Phe Ser Pro Glu 
        195                 200                 205             


Cys Phe Ser Asp Thr Pro Gly Glu Phe Ala Val Glu Ile Cys Glu Ala 
    210                 215                 220                 


Val Lys Lys Ala Trp Glu Pro Thr Glu Glu Asn Pro Ile Ile Phe Asn 
225                 230                 235                 240 


Leu Pro Ala Thr Val Glu Val Ala Ser Pro Asn Val Tyr Ala Asp Gln 
                245                 250                 255     


Ile Glu Tyr Phe Ala Thr His Ile Thr Glu Arg Glu Lys Val Cys Ile 
            260                 265                 270         


Ser Thr His Cys His Asn Asp Arg Gly Cys Gly Val Ala Ala Thr Glu 
        275                 280                 285             


Leu Gly Met Leu Ala Gly Ala Asp Arg Val Glu Gly Cys Leu Phe Gly 
    290                 295                 300                 


Asn Gly Glu Arg Thr Gly Asn Val Asp Leu Val Thr Val Ala Met Asn 
305                 310                 315                 320 


Met Tyr Thr Gln Gly Val Ser Pro Asn Leu Asp Phe Ser Asp Leu Thr 
                325                 330                 335     


Ser Val Leu Asp Val Val Glu Arg Cys Asn Lys Ile Pro Val Ser Gln 
            340                 345                 350         


Arg Ala Pro Tyr Gly Gly Asp Leu Val Val Cys Ala Phe Ser Gly Ser 
        355                 360                 365             


His Gln Asp Ala Ile Lys Lys Gly Phe Asn Leu Gln Asn Lys Lys Arg 
    370                 375                 380                 


Ala Gln Gly Glu Thr Gln Trp Arg Ile Pro Tyr Leu Pro Leu Asp Pro 
385                 390                 395                 400 


Lys Asp Ile Gly Arg Asp Tyr Glu Ala Val 
                405                 410 


<210>  3
<211>  423
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  3

Met Val Lys Glu Ser Ile Ile Ala Leu Ala Glu His Ala Ala Ser Arg 
1               5                   10                  15      


Ala Ser Arg Val Ile Pro Pro Val Lys Leu Ala Tyr Lys Asn Met Leu 
            20                  25                  30          


Lys Asp Pro Ser Ser Lys Tyr Lys Pro Phe Asn Ala Pro Lys Leu Ser 
        35                  40                  45              


Asn Arg Lys Trp Pro Asp Asn Arg Ile Thr Arg Ala Pro Arg Trp Leu 
    50                  55                  60                  


Ser Thr Asp Leu Arg Asp Gly Asn Gln Ser Leu Pro Asp Pro Met Ser 
65                  70                  75                  80  


Val Glu Gln Lys Lys Glu Tyr Phe His Lys Leu Val Asn Ile Gly Phe 
                85                  90                  95      


Lys Glu Ile Glu Val Ser Phe Pro Ser Ala Ser Gln Thr Asp Phe Asp 
            100                 105                 110         


Phe Thr Arg Tyr Ala Val Glu Asn Ala Pro Asp Asp Val Ser Ile Gln 
        115                 120                 125             


Cys Leu Val Gln Ser Arg Glu His Leu Ile Lys Arg Thr Val Glu Ala 
    130                 135                 140                 


Leu Thr Gly Ala Lys Lys Ala Thr Ile His Thr Tyr Leu Ala Thr Ser 
145                 150                 155                 160 


Asp Met Phe Arg Glu Ile Val Phe Asn Met Ser Arg Glu Glu Ala Ile 
                165                 170                 175     


Ser Lys Ala Val Glu Ala Thr Lys Leu Val Arg Lys Leu Thr Lys Asp 
            180                 185                 190         


Asp Pro Ser Gln Gln Ala Thr Arg Trp Ser Tyr Glu Phe Ser Pro Glu 
        195                 200                 205             


Cys Phe Ser Asp Thr Pro Gly Glu Phe Ala Val Glu Ile Cys Glu Ala 
    210                 215                 220                 


Val Lys Lys Ala Trp Glu Pro Thr Glu Glu Asn Pro Ile Ile Phe Asn 
225                 230                 235                 240 


Leu Pro Ala Thr Val Glu Val Ala Ser Pro Asn Val Tyr Ala Asp Gln 
                245                 250                 255     


Ile Glu Tyr Phe Ala Thr His Ile Thr Glu Arg Glu Lys Val Cys Ile 
            260                 265                 270         


Ser Thr His Cys His Asn Asp Arg Gly Cys Gly Val Ala Ala Thr Glu 
        275                 280                 285             


Leu Gly Met Leu Ala Gly Ala Asp Arg Val Glu Gly Cys Leu Phe Gly 
    290                 295                 300                 


Asn Gly Glu Arg Thr Gly Asn Val Asp Leu Val Thr Val Ala Met Asn 
305                 310                 315                 320 


Met Tyr Thr Gln Gly Val Ser Pro Asn Leu Asp Phe Ser Asp Leu Thr 
                325                 330                 335     


Ser Val Leu Asp Val Val Glu Arg Cys Asn Lys Ile Pro Val Ser Gln 
            340                 345                 350         


Arg Ala Pro Tyr Gly Gly Asp Leu Val Val Cys Ala Phe Ser Gly Ser 
        355                 360                 365             


His Gln Asp Ala Ile Lys Lys Gly Phe Asn Leu Gln Asn Lys Lys Arg 
    370                 375                 380                 


Ala Gln Gly Glu Thr Gln Trp Arg Ile Pro Tyr Leu Pro Leu Asp Pro 
385                 390                 395                 400 


Lys Asp Ile Gly Arg Asp Tyr Glu Ala Val Ile Arg Val Asn Ser Gln 
                405                 410                 415     


Ser Gly Lys Gly Gly Ala Ala 
            420             


