б
<110>  Ͼҩҵ޹˾
<120>  һǿͷȾؼ
<130>  P150002P
<150>  CN 201410017909.8
<151>  2014-1-15
<160>  4
<170>  PatentIn version 3.3
<210>  1

<211>  520
<212>  PRT
<213>  HPV18 E6/HPV18 E7/HPV16 E6/HPV16 E7ںϵװ
<400>  1
Met Ala Arg Phe Glu Asp Pro Thr Arg Arg Pro Tyr Lys Leu Pro Asp 
1               5                   10                  15      

Leu Cys Thr Glu Leu Asn Thr Ser Leu Gln Asp Ile Glu Ile Thr Cys 
            20                  25                  30          

Val Tyr Cys Lys Thr Val Leu Glu Leu Thr Glu Val Phe Glu Phe Ala 
        35                  40                  45              

Phe Lys Asp Leu Phe Val Val Tyr Arg Asp Ser Ile Pro His Ala Ala 
    50                  55                  60                  

Cys His Lys Cys Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg His 
65                  70                  75                  80  

Tyr Ser Asp Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn Thr 
                85                  90                  95      

Gly Leu Tyr Asn Leu Leu Ile Arg Cys Leu Arg Cys Gln Lys Pro Leu 
            100                 105                 110         

Asn Pro Ala Gly Gly Leu Arg His Leu Asn Glu Lys Arg Arg Phe His 
        115                 120                 125             

Asn Ile Ala Gly His Tyr Arg Gly Gln Cys His Ser Cys Cys Asn Arg 
    130                 135                 140                 

Ala Arg Gln Glu Arg Leu Gln Arg Arg Arg Glu Thr Gln Val Met His 
145                 150                 155                 160 

Gly Pro Lys Ala Thr Leu Gln Asp Ile Val Leu His Leu Glu Pro Gln 
                165                 170                 175     

Asn Glu Ile Pro Val Asp Leu Leu Gly His Gly Gln Leu Ser Asp Ser 
            180                 185                 190         

Glu Glu Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His Leu Pro 
        195                 200                 205             

Ala Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys Met Cys Cys 
    210                 215                 220                 

Lys Cys Glu Ala Arg Ile Glu Leu Val Val Glu Ser Ser Ala Asp Asp 
225                 230                 235                 240 

Leu Arg Ala Phe Gln Gln Leu Phe Leu Asn Thr Leu Ser Phe Val Cys 
                245                 250                 255     
Pro Trp Cys Ala Ser Gln Gln Ser Met His Gln Lys Arg Thr Ala Met 
            260                 265                 270         
Phe Gln Asp Pro Gln Glu Arg Pro Arg Lys Leu Pro Gln Leu Cys Thr 
        275                 280                 285             
Glu Leu Gln Thr Thr Ile His Asp Ile Ile Leu Glu Cys Val Tyr Cys 
    290                 295                 300                 
Lys Gln Gln Leu Leu Arg Arg Glu Val Tyr Asp Phe Ala Phe Arg Asp 
305                 310                 315                 320 
Leu Cys Ile Val Tyr Arg Asp Gly Asn Pro Tyr Ala Val Cys Asp Lys 
                325                 330                 335     
Cys Leu Lys Phe Tyr Ser Lys Ile Ser Glu Tyr Arg His Tyr Cys Tyr 
            340                 345                 350         
Ser Leu Tyr Gly Thr Thr Leu Glu Gln Gln Tyr Asn Lys Pro Leu Cys 
        355                 360                 365             
Asp Leu Leu Ile Arg Cys Ile Asn Cys Gln Lys Pro Leu Cys Pro Glu 
    370                 375                 380                 
Gly Gly Gln Arg His Leu Asp Lys Lys Gln Arg Phe His Asn Ile Arg 
385                 390                 395                 400 
Gly Arg Trp Thr Gly Arg Cys Met Ser Cys Cys Arg Ser Ser Arg Thr 
                405                 410                 415     
Arg Arg Glu Thr Gln Leu Met His Gly Asp Thr Pro Thr Leu His Glu 
            420                 425                 430         
Tyr Met Leu Asp Leu Gln Pro Glu Thr Thr Asp Leu Tyr Gly Tyr Gly 
        435                 440                 445             
Gln Leu Asn Asp Ser Ser Glu Glu Glu Asp Glu Ile Asp Gly Pro Ala 
    450                 455                 460                 
Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile Val Thr Phe Cys 
465                 470                 475                 480 
Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys Val Gln Ser Thr His Val 
                485                 490                 495     
Asp Ile Arg Thr Leu Glu Asp Leu Leu Met Gly Thr Leu Gly Ile Val 
            500                 505                 510         
Cys Pro Ile Cys Ser Gln Lys Pro 
        515                 520 

<210>  2
<211>  1145
<212>  PRT
<213>  HPV18 E6/HPV18 E7/HPV16 E6/HPV16 E7/HSP70ںϵװ

<400>  2
Met Ala Arg Phe Glu Asp Pro Thr Arg Arg Pro Tyr Lys Leu Pro Asp 
1               5                   10                  15      
Leu Cys Thr Glu Leu Asn Thr Ser Leu Gln Asp Ile Glu Ile Thr Cys 
            20                  25                  30          
Val Tyr Cys Lys Thr Val Leu Glu Leu Thr Glu Val Phe Glu Phe Ala 
        35                  40                  45              
Phe Lys Asp Leu Phe Val Val Tyr Arg Asp Ser Ile Pro His Ala Ala 
    50                  55                  60                  
Cys His Lys Cys Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg His 
65                  70                  75                  80  
Tyr Ser Asp Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn Thr 
                85                  90                  95      
Gly Leu Tyr Asn Leu Leu Ile Arg Cys Leu Arg Cys Gln Lys Pro Leu 
            100                 105                 110         
Asn Pro Ala Gly Gly Leu Arg His Leu Asn Glu Lys Arg Arg Phe His 
        115                 120                 125             
Asn Ile Ala Gly His Tyr Arg Gly Gln Cys His Ser Cys Cys Asn Arg 
    130                 135                 140                 
Ala Arg Gln Glu Arg Leu Gln Arg Arg Arg Glu Thr Gln Val Met His 
145                 150                 155                 160 
Gly Pro Lys Ala Thr Leu Gln Asp Ile Val Leu His Leu Glu Pro Gln 
                165                 170                 175     
Asn Glu Ile Pro Val Asp Leu Leu Gly His Gly Gln Leu Ser Asp Ser 
            180                 185                 190         
Glu Glu Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His Leu Pro 
        195                 200                 205             
Ala Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys Met Cys Cys 
    210                 215                 220                 
Lys Cys Glu Ala Arg Ile Glu Leu Val Val Glu Ser Ser Ala Asp Asp 
225                 230                 235                 240 
Leu Arg Ala Phe Gln Gln Leu Phe Leu Asn Thr Leu Ser Phe Val Cys 
                245                 250                 255     
Pro Trp Cys Ala Ser Gln Gln Ser Met His Gln Lys Arg Thr Ala Met 
            260                 265                 270         
Phe Gln Asp Pro Gln Glu Arg Pro Arg Lys Leu Pro Gln Leu Cys Thr 
        275                 280                 285             
Glu Leu Gln Thr Thr Ile His Asp Ile Ile Leu Glu Cys Val Tyr Cys 
    290                 295                 300                 
Lys Gln Gln Leu Leu Arg Arg Glu Val Tyr Asp Phe Ala Phe Arg Asp 
305                 310                 315                 320 
Leu Cys Ile Val Tyr Arg Asp Gly Asn Pro Tyr Ala Val Cys Asp Lys 
                325                 330                 335     
Cys Leu Lys Phe Tyr Ser Lys Ile Ser Glu Tyr Arg His Tyr Cys Tyr 
            340                 345                 350         
Ser Leu Tyr Gly Thr Thr Leu Glu Gln Gln Tyr Asn Lys Pro Leu Cys 
        355                 360                 365             
Asp Leu Leu Ile Arg Cys Ile Asn Cys Gln Lys Pro Leu Cys Pro Glu 
    370                 375                 380                 
Gly Gly Gln Arg His Leu Asp Lys Lys Gln Arg Phe His Asn Ile Arg 
385                 390                 395                 400 
Gly Arg Trp Thr Gly Arg Cys Met Ser Cys Cys Arg Ser Ser Arg Thr 
                405                 410                 415     
Arg Arg Glu Thr Gln Leu Met His Gly Asp Thr Pro Thr Leu His Glu 
            420                 425                 430         
Tyr Met Leu Asp Leu Gln Pro Glu Thr Thr Asp Leu Tyr Gly Tyr Gly 
        435                 440                 445             
Gln Leu Asn Asp Ser Ser Glu Glu Glu Asp Glu Ile Asp Gly Pro Ala 
    450                 455                 460                 
Gly Gln Ala Glu Pro Asp Arg Ala His Tyr Asn Ile Val Thr Phe Cys 
465                 470                 475                 480 
Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys Val Gln Ser Thr His Val 
                485                 490                 495     
Asp Ile Arg Thr Leu Glu Asp Leu Leu Met Gly Thr Leu Gly Ile Val 
            500                 505                 510         
Cys Pro Ile Cys Ser Gln Lys Pro Met Ala Arg Ala Val Gly Ile Asp 
        515                 520                 525             
Leu Gly Thr Thr Asn Ser Val Val Ser Val Leu Glu Gly Gly Asp Pro 
    530                 535                 540                 
Val Val Val Ala Asn Ser Glu Gly Ser Arg Thr Thr Pro Ser Ile Val 
545                 550                 555                 560 
Ala Phe Ala Arg Asn Gly Glu Val Leu Val Gly Gln Pro Ala Lys Asn 
                565                 570                 575     
Gln Ala Val Thr Asn Val Asp Arg Thr Val Arg Ser Val Lys Arg His 
            580                 585                 590         
Met Gly Ser Asp Trp Ser Ile Glu Ile Asp Gly Lys Lys Tyr Thr Ala 
        595                 600                 605             
Pro Glu Ile Ser Ala Arg Ile Leu Met Lys Leu Lys Arg Asp Ala Glu 
    610                 615                 620                 
Ala Tyr Leu Gly Glu Asp Ile Thr Asp Ala Val Ile Thr Thr Pro Ala 
625                 630                 635                 640 
Tyr Phe Asn Asp Ala Gln Arg Gln Ala Thr Lys Asp Ala Gly Gln Ile 
                645                 650                 655     
Ala Gly Leu Asn Val Leu Arg Ile Val Asn Glu Pro Thr Ala Ala Ala 
            660                 665                 670         
Leu Ala Tyr Gly Leu Asp Lys Gly Glu Lys Glu Gln Arg Ile Leu Val 
        675                 680                 685             
Phe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser Leu Leu Glu Ile Gly 
    690                 695                 700                 
Glu Gly Val Val Glu Val Arg Ala Thr Ser Gly Asp Asn His Leu Gly 
705                 710                 715                 720 
Gly Asp Asp Trp Asp Gln Arg Val Val Asp Trp Leu Val Asp Lys Phe 
                725                 730                 735     
Lys Gly Thr Ser Gly Ile Asp Leu Thr Lys Asp Lys Met Ala Met Gln 
            740                 745                 750         
Arg Leu Arg Glu Ala Ala Glu Lys Ala Lys Ile Glu Leu Ser Ser Ser 
        755                 760                 765             
Gln Ser Thr Ser Ile Asn Leu Pro Tyr Ile Thr Val Asp Ala Asp Lys 
    770                 775                 780                 
Asn Pro Leu Phe Leu Asp Glu Gln Leu Thr Arg Ala Glu Phe Gln Arg 
785                 790                 795                 800 
Ile Thr Gln Asp Leu Leu Asp Arg Thr Arg Lys Pro Phe Gln Ser Val 
                805                 810                 815     
Ile Ala Asp Thr Gly Ile Ser Val Ser Glu Ile Asp His Val Val Leu 
            820                 825                 830         
Val Gly Gly Ser Thr Arg Met Pro Ala Val Thr Asp Leu Val Lys Glu 
        835                 840                 845             
Leu Thr Gly Gly Lys Glu Pro Asn Lys Gly Val Asn Pro Asp Glu Val 
    850                 855                 860                 
Val Ala Val Gly Ala Ala Leu Gln Ala Gly Val Leu Lys Gly Glu Val 
865                 870                 875                 880 
Lys Asp Val Leu Leu Leu Asp Val Thr Pro Leu Ser Leu Gly Ile Glu 
                885                 890                 895     
Thr Lys Gly Gly Val Met Thr Arg Leu Ile Glu Arg Asn Thr Thr Ile 
            900                 905                 910         
Pro Thr Lys Arg Ser Glu Ser Phe Thr Thr Ala Asp Asp Asn Gln Pro 
        915                 920                 925             
Ser Val Gln Ile Gln Val Tyr Gln Gly Glu Arg Glu Ile Ala Ala His 
    930                 935                 940                 
Asn Lys Leu Leu Gly Ser Phe Glu Leu Thr Gly Ile Pro Pro Ala Pro 
945                 950                 955                 960 
Arg Gly Ile Pro Gln Ile Glu Val Thr Phe Asp Ile Asp Ala Asn Gly 
                965                 970                 975     
Ile Val His Val Thr Ala Lys Asp Lys Gly Thr Gly Lys Glu Asn Thr 
            980                 985                 990         
Ile Arg Ile Gln Glu Gly Ser Gly  Leu Ser Lys Glu Asp  Ile Asp Arg 
        995                 1000                 1005             
Met Ile  Lys Asp Ala Glu Ala  His Ala Glu Glu Asp  Arg Lys Arg 
    1010                 1015                 1020             
Arg Glu  Glu Ala Asp Val Arg  Asn Gln Ala Glu Thr  Leu Val Tyr 
    1025                 1030                 1035             
Gln Thr  Glu Lys Phe Val Lys  Glu Gln Arg Glu Ala  Glu Gly Gly 
    1040                 1045                 1050             
Ser Lys  Val Pro Glu Asp Thr  Leu Asn Lys Val Asp  Ala Ala Val 
    1055                 1060                 1065             
Ala Glu  Ala Lys Ala Ala Leu  Gly Gly Ser Asp Ile  Ser Ala Ile 
    1070                 1075                 1080             
Lys Ser  Ala Met Glu Lys Leu  Gly Gln Glu Ser Gln  Ala Leu Gly 
    1085                 1090                 1095             
Gln Ala  Ile Tyr Glu Ala Ala  Gln Ala Ala Ser Gln  Ala Thr Gly 
    1100                 1105                 1110             
Ala Ala  His Pro Gly Gly Glu  Pro Gly Gly Ala His  Pro Gly Ser 
    1115                 1120                 1125             
Ala Asp  Asp Val Val Asp Ala  Glu Val Val Asp Asp  Gly Arg Glu 
    1130                 1135                 1140             
Ala Lys  
    1145 

<210>  3
<211>  3438
<212>  DNA
<213>  HPV18&16/E6/E7HSP

<400>  3
atggccagat tcgaggaccc caccagacgg ccctacaagc tgcctgatct gtgcaccgag     60
ctgaacacca gcctgcagga catcgagatc acctgtgtgt actgcaagac cgtgctggaa    120
ctgaccgagg tgttcgagtt cgccttcaag gacctgttcg tggtgtaccg ggacagcatc    180
cctcacgccg cctgccacaa gtgcatcgac ttctacagcc ggatcagaga gctgcggcac    240
tacagcgaca gcgtgtacgg cgacaccctg gaaaagctga ccaacaccgg cctgtacaac    300
ctgctgatcc ggtgcctgag atgccagaag cccctgaatc ctgccggcgg actgagacac    360
ctgaacgaga agcggcggtt ccacaatatc gccggccact acagaggcca gtgccacagc    420
tgctgcaacc gggccagaca ggaacggctg cagcggagaa gagaaaccca agtgatgcac    480
ggccccaagg ccaccctgca ggatattgtg ctgcacctgg aaccccagaa cgagatcccc    540
gtggatctgc tgggacacgg ccagctgagc gactccgagg aagagaacga cgagatcgac    600
ggcgtgaacc accagcatct gcctgccaga agggccgagc ctcagagaca caccatgctg    660
tgcatgtgct gcaagtgcga ggcccggatc gagctggtgg tggaaagcag cgccgacgac    720
ctgagagcct tccagcagct gttcctgaac accctgagct tcgtgtgccc ttggtgcgcc    780
agccagcaga gcatgcacca gaaacggacc gccatgttcc aggaccccca ggaaagaccc    840
agaaagctgc cccagctgtg taccgaactg cagaccacca tccacgacat catcctggaa    900
tgcgtgtact gtaaacagca gctgctgagg cgcgaggtgt acgactttgc ctttcgggac    960
ctgtgcatcg tgtacaggga cggcaacccc tacgccgtgt gcgacaagtg cctgaagttc   1020
tacagcaaga tcagcgagta ccgccactac tgctactccc tgtacggcac cacactggaa   1080
cagcagtaca acaagcccct gtgcgatctg ctgatcagat gcatcaactg ccagaaacct   1140
ctgtgccccg agggcggcca gaggcacctg gataagaagc agagattcca caacatccgg   1200
ggcagatgga ccggcagatg catgtcctgc tgcagaagca gccggaccag acgggaaaca   1260
cagctgatgc atggcgatac ccctaccctg cacgagtaca tgctggacct gcagcccgag   1320
acaaccgatc tgtacggcta cggacagctg aacgacagct ctgaagaaga ggacgaaatt   1380
gacggccctg ccggccaggc cgaacctgat agagcccact acaatatcgt gaccttctgt   1440
tgcaagtgtg acagcaccct gcggctgtgc gtgcagagca cacacgtgga catccggacc   1500
ctggaagatc tgctgatggg caccctgggc atcgtgtgtc ccatctgcag ccagaaaccc   1560
atggccagag ccgtgggcat cgatctgggc accaccaaca gcgtggtgtc tgtgctggaa   1620
gggggcgacc ctgtggtggt ggccaatagc gagggcagca gaaccacccc tagcatcgtg   1680
gccttcgccc ggaatggcga agtgctcgtg ggacagcccg ccaagaatca ggccgtgacc   1740
aacgtggaca gaaccgtgcg gagcgtgaag cggcacatgg gcagcgattg gagcatcgag   1800
attgacggca agaagtacac cgcccctgag atcagcgccc ggatcctgat gaagctgaag   1860
agggacgccg aggcctacct gggcgaggat atcaccgatg ccgtgatcac cacccccgcc   1920
tacttcaacg acgcccagag acaggccacc aaggacgccg gacagatcgc cggactgaac   1980
gtgctgcgga tcgtgaacga gcctacagcc gccgctctgg cctacggact ggacaagggc   2040
gagaaagaac agcggatcct ggtgttcgac ctgggcggag gcaccttcga tgtgtccctg   2100
ctggaaatcg gcgagggcgt ggtggaagtg cgggccacat ctggcgataa ccacctggga   2160
ggcgacgact gggaccagag agtggtggac tggctggtgg acaagttcaa gggcaccagc   2220
ggcatcgacc tgaccaagga caagatggcc atgcagcggc tgagagaggc cgccgagaag   2280
gccaagattg agctgagcag cagccagagc acctccatca acctgcccta catcaccgtg   2340
gacgccgaca agaaccccct gttcctggac gagcagctga ccagagccga gttccagcgg   2400
atcacccagg acctgctgga caggaccaga aagcccttcc agagcgtgat cgccgacacc   2460
ggcatcagcg tgtccgagat cgatcacgtg gtgctcgtgg gcggcagcac cagaatgcct   2520
gccgtgaccg acctcgtgaa agagctgacc ggcggcaaag aacccaacaa gggcgtgaac   2580
cccgacgagg tggtggctgt gggagctgca ctgcaggcag gcgtgctgaa gggcgaagtg   2640
aaggacgtgc tgctgctgga cgtgacccct ctgagcctgg gaatcgagac aaagggcgga   2700
gtgatgaccc ggctgatcga gagaaacacc acaatcccca ccaagcggag cgagagcttc   2760
accaccgccg acgataacca gcccagcgtg cagatccagg tgtaccaggg cgagagagag   2820
atcgccgccc acaacaagct gctgggcagc ttcgagctga caggcatccc accagccccc   2880
agaggaatcc ctcagatcga agtgaccttc gacatcgacg ccaacggcat cgtgcacgtg   2940
accgccaagg ataagggcac cggcaaagag aacaccatcc ggatccagga aggcagcggc   3000
ctgagcaaag aggatatcga cagaatgatc aaggatgccg aggcccacgc cgaagaggac   3060
cggaaaagac gggaagaggc cgacgtgcgg aaccaggctg agacactggt gtatcagacc   3120
gagaagtttg tgaaagagca gcgcgaggcc gagggcggat ctaaggtgcc agaggacacc   3180
ctgaacaagg tggacgctgc cgtggccgaa gccaaggctg ctctgggagg cagcgatatc   3240
agcgccatca agagcgccat ggaaaaactg ggccaggaaa gccaggccct ggggcaggcc   3300
atctatgaag ctgctcaggc tgccagccag gctacaggcg ctgctcatcc tggcggagaa   3360
ccaggcggag cccatccagg atctgccgat gacgtggtgg atgccgaagt ggtggacgac   3420
ggccgggaag ccaaatga                                                 3438

<210>  4
<211>  435
<212>  DNA
<213>  GM-CSF

<400>  4
atgtggctgc agagcctgct gctcttgggc actgtggcct gcagcatctc tgcacccgcc     60
cgctcgccca gccccagcac gcagccctgg gagcatgtga atgccatcca ggaggcccgg    120
cgtctcctga acctgagtag agacactgct gctgagatga atgaaacagt agaagtcatc    180
tcagaaatgt ttgacctcca ggagccgacc tgcctacaga cccgcctgga gctgtacaag    240
cagggcctgc ggggcagcct caccaagctc aagggcccct tgaccatgat ggccagccac    300
tacaagcagc actgccctcc aaccccggaa acttcctgtg caacccagat tatcaccttt    360
gaaagtttca aagagaacct gaaggacttt ctgcttgtca tcccctttga ctgctgggag    420
ccagtccagg agtga                                                     435
