                         SEQUENCE LISTING

<110>  Orla Protein Technologies Limited
 
<120>  PROTEIN COATED POLYMERIC SUBSTRATE

<130>  PS/P213114WO

<140>  GB1400562.3
<141>  2014-01-14

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  594
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMP0 "wild-type" modified by removal of signal and C-terminal 
       domain and addition of N-terminal his tag and cysteine at 
       position 10.

<400>  1
atgcatcacc atcaccatca ctcgagctgt gctccgaaag ataacacctg gtacactggt       60

gctaaactgg gctggtccca gtaccatgac actggtttca tcaacaacaa tggcccgacc      120

catgaaaacc aactgggcgc tggtgctttt ggtggttacc aggttaaccc gtatgttggc      180

tttgaaatgg gttacgactg gttaggtcgt atgccgtaca aaggcagcgt tgaaaacggt      240

gcatacaaag ctcagggcgt tcaactgacc gctaaactgg gttacccaat cactgacgac      300

ctggacatct acactcgtct gggtggcatg gtatggcgtg cagacactaa atccaacgtt      360

tatggtaaaa accacgacac cggcgtttct ccggtcttcg ctggcggtgt tgagtacgcg      420

atcactcctg aaatcgctac ccgtctggaa taccagtgga ccaacaacat cggtgacgca      480

cacaccatcg gcactcgtcc ggacaacggc atgctgagcc tgggtgtttc ctaccgtttc      540

ggtcagggcg aagcagctcc agtagttgct ccggctccag ctccggcacc gtaa            594


<210>  2
<211>  197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP0 "wild-type" modified by removal of signal and C-terminal 
       domain and addition of N-terminal his tag and cysteine at 
       position 10.

<400>  2

Met His His His His His His Ser Ser Cys Ala Pro Lys Asp Asn Thr 
1               5                   10                  15      


Trp Tyr Thr Gly Ala Lys Leu Gly Trp Ser Gln Tyr His Asp Thr Gly 
            20                  25                  30          


Phe Ile Asn Asn Asn Gly Pro Thr His Glu Asn Gln Leu Gly Ala Gly 
        35                  40                  45              


Ala Phe Gly Gly Tyr Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly 
    50                  55                  60                  


Tyr Asp Trp Leu Gly Arg Met Pro Tyr Lys Gly Ser Val Glu Asn Gly 
65                  70                  75                  80  


Ala Tyr Lys Ala Gln Gly Val Gln Leu Thr Ala Lys Leu Gly Tyr Pro 
                85                  90                  95      


Ile Thr Asp Asp Leu Asp Ile Tyr Thr Arg Leu Gly Gly Met Val Trp 
            100                 105                 110         


Arg Ala Asp Thr Lys Ser Asn Val Tyr Gly Lys Asn His Asp Thr Gly 
        115                 120                 125             


Val Ser Pro Val Phe Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu 
    130                 135                 140                 


Ile Ala Thr Arg Leu Glu Tyr Gln Trp Thr Asn Asn Ile Gly Asp Ala 
145                 150                 155                 160 


His Thr Ile Gly Thr Arg Pro Asp Asn Gly Met Leu Ser Leu Gly Val 
                165                 170                 175     


Ser Tyr Arg Phe Gly Gln Gly Glu Ala Ala Pro Val Val Ala Pro Ala 
            180                 185                 190         


Pro Ala Pro Ala Pro 
        195         


<210>  3
<211>  558
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMP9 Loop 1 circular permutation

<400>  3
atgcatcacc atcaccatca ctcgagtgaa aaccaactgg gcgctggtgc ttttggtggt       60

taccaggtta acccgtatgt tggctttgaa atgggttacg actggttagg tcgtatgccg      120

tacaaaggca gcgttgaaaa cggtgcatac aaagctcagg gcgttcaact gaccgctaaa      180

ctgggttacc caatcactga cgacctggac atctacactc gtttgggtgg catggtatgg      240

cgtgcagaca ctaaatccaa cgtttatggt aaaaaccacg acaccggcgt ttctccggtc      300

ttcgctggcg gtgttgagta cgcgatcact cctgaaatcg ctacccgtct ggaataccag      360

tggaccaaca acatcggtga cgcacacacc atcggcactc gtccggacaa cggcatgctg      420

agcctgggtg tttcctaccg tttcggtccg tgtacaggtg atacctggta cactggtgct      480

aaactgggct ggtcccagta ccatgacact ggtttcatca acaacaatgg cccaacccat      540

acgcgtgagg aattttga                                                    558


<210>  4
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP9 Loop 1 circular permutation

<400>  4

Met His His His His His His Ser Ser Glu Asn Gln Leu Gly Ala Gly 
1               5                   10                  15      


Ala Phe Gly Gly Tyr Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly 
            20                  25                  30          


Tyr Asp Trp Leu Gly Arg Met Pro Tyr Lys Gly Ser Val Glu Asn Gly 
        35                  40                  45              


Ala Tyr Lys Ala Gln Gly Val Gln Leu Thr Ala Lys Leu Gly Tyr Pro 
    50                  55                  60                  


Ile Thr Asp Asp Leu Asp Ile Tyr Thr Arg Leu Gly Gly Met Val Trp 
65                  70                  75                  80  


Arg Ala Asp Thr Lys Ser Asn Val Tyr Gly Lys Asn His Asp Thr Gly 
                85                  90                  95      


Val Ser Pro Val Phe Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu 
            100                 105                 110         


Ile Ala Thr Arg Leu Glu Tyr Gln Trp Thr Asn Asn Ile Gly Asp Ala 
        115                 120                 125             


His Thr Ile Gly Thr Arg Pro Asp Asn Gly Met Leu Ser Leu Gly Val 
    130                 135                 140                 


Ser Tyr Arg Phe Gly Pro Cys Thr Gly Asp Thr Trp Tyr Thr Gly Ala 
145                 150                 155                 160 


Lys Leu Gly Trp Ser Gln Tyr His Asp Thr Gly Phe Ile Asn Asn Asn 
                165                 170                 175     


Gly Pro Thr His Thr Arg Glu Glu Phe 
            180                 185 


<210>  5
<211>  555
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   OMP13 Loop 3 circular permutation

<400>  5
atgcatcacc atcaccatca ctcgagttct ccggtcttcg ctggcggtgt tgagtacgcg       60

atcactcctg aaatcgctac ccgtctggaa taccagtgga ccaacaacat cggtgacgca      120

cacaccatcg gcactcgtcc ggacaacggc atgctgagcc tgggtgtttc ctaccgtttc      180

ggtccgtgta caggtgatac ctggtacact ggtgctaaac tgggctggtc ccagtaccat      240

gacactggtt tcatcaacaa caatggcccg acccatgaaa accaactggg cgctggtgct      300

tttggtggtt accaggttaa cccgtatgtt ggctttgaaa tgggttacga ctggttaggt      360

cgtatgccgt acaaaggcag cgttgaaaac ggtgcataca aagctcaggg cgttcaactg      420

accgctaaac tgggttaccc aatcactgac gacctggaca tctacactcg tctgggtggc      480

atggtatggc gtgcagacac taaatccaac gtttatggta aaaaccacga caccggcacg      540

cgtgaggaat tttga                                                       555


<210>  6
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP13 Loop 3 circular permutation

<400>  6

Met His His His His His His Ser Ser Ser Pro Val Phe Ala Gly Gly 
1               5                   10                  15      


Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu Glu Tyr Gln 
            20                  25                  30          


Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr Arg Pro Asp 
        35                  40                  45              


Asn Gly Met Leu Ser Leu Gly Val Ser Tyr Arg Phe Gly Pro Cys Thr 
    50                  55                  60                  


Gly Asp Thr Trp Tyr Thr Gly Ala Lys Leu Gly Trp Ser Gln Tyr His 
65                  70                  75                  80  


Asp Thr Gly Phe Ile Asn Asn Asn Gly Pro Thr His Glu Asn Gln Leu 
                85                  90                  95      


Gly Ala Gly Ala Phe Gly Gly Tyr Gln Val Asn Pro Tyr Val Gly Phe 
            100                 105                 110         


Glu Met Gly Tyr Asp Trp Leu Gly Arg Met Pro Tyr Lys Gly Ser Val 
        115                 120                 125             


Glu Asn Gly Ala Tyr Lys Ala Gln Gly Val Gln Leu Thr Ala Lys Leu 
    130                 135                 140                 


Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr Thr Arg Leu Gly Gly 
145                 150                 155                 160 


Met Val Trp Arg Ala Asp Thr Lys Ser Asn Val Tyr Gly Lys Asn His 
                165                 170                 175     


Asp Thr Gly Thr Arg Glu Glu Phe 
            180                 


<210>  7
<211>  555
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMP14 Loop 4 circular permutation

<400>  7
atgcatcacc atcaccatca ctcgagcggc atgctgagcc tgggtgtttc ctaccgtttc       60

ggtccgtgta caggtgatac ctggtacact ggtgctaaac tgggctggtc ccagtaccat      120

gacactggtt tcatcaacaa caatggcccg acccatgaaa accaactggg cgctggtgct      180

tttggtggtt accaggttaa cccgtatgtt ggctttgaaa tgggttacga ctggttaggt      240

cgtatgccgt acaaaggcag cgttgaaaac ggtgcataca aagctcaggg cgttcaactg      300

accgctaaac tgggttaccc aatcactgac gacctggaca tctacactcg tctgggtggc      360

atggtatggc gtgcagacac taaatccaac gtttatggta aaaaccacga caccggcgtt      420

tctccggtct tcgctggcgg tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg      480

gaataccagt ggaccaacaa catcggtgac gcacacacca tcggcactcg tccggacacg      540

cgtgaggaat tttga                                                       555


<210>  8
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP 14 Loop 4 circular permutation

<400>  8

Met His His His His His His Ser Ser Gly Met Leu Ser Leu Gly Val 
1               5                   10                  15      


Ser Tyr Arg Phe Gly Pro Cys Thr Gly Asp Thr Trp Tyr Thr Gly Ala 
            20                  25                  30          


Lys Leu Gly Trp Ser Gln Tyr His Asp Thr Gly Phe Ile Asn Asn Asn 
        35                  40                  45              


Gly Pro Thr His Glu Asn Gln Leu Gly Ala Gly Ala Phe Gly Gly Tyr 
    50                  55                  60                  


Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly 
65                  70                  75                  80  


Arg Met Pro Tyr Lys Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln 
                85                  90                  95      


Gly Val Gln Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu 
            100                 105                 110         


Asp Ile Tyr Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys 
        115                 120                 125             


Ser Asn Val Tyr Gly Lys Asn His Asp Thr Gly Val Ser Pro Val Phe 
    130                 135                 140                 


Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu 
145                 150                 155                 160 


Glu Tyr Gln Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr 
                165                 170                 175     


Arg Pro Asp Thr Arg Glu Glu Phe 
            180                 


<210>  9
<211>  612
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMP140	short loops, alpha helical spacer, c-terminal his tag, no 
       cysteine

<400>  9
atgtcctcga gcgtagacaa caaattcaac aaagaaaaaa aaaacgcatt ctatgagatc       60

ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagcg      120

gccccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatga tgctcaggcg      180

ccgaaagtag acgcgaattc gagcgaaaac caactgggcg ctggtgcttt tggtggttac      240

caggttaacc cgtatgttgg ctttgaaatg ggttacgact ggttaggtcg tatgccgtac      300

aaagctcagg gcgttcaact gaccgctaaa ctgggttacc caatcactga cgacctggac      360

atctacactc gtttgggtgg catggtatgg cgtgcagaca ctggcgtttc tccggtcttc      420

gctggcggtg ttgagtacgc gatcactcct gaaatcgcta cccgtctgga ataccagtgg      480

accaacaaca tcggtgacaa cggcatgctg agcctgggtg tttcctaccg tttcggtccg      540

atcacaggtg atacctggta cactggtgct aaactgggct ggtcccagta ccatcaccat      600

caccatcact ga                                                          612


<210>  10
<211>  203
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP140	short loops, alpha helical spacer, c-terminal his tag, no 
       cysteine

<400>  10

Met Ser Ser Ser Val Asp Asn Lys Phe Asn Lys Glu Lys Lys Asn Ala 
1               5                   10                  15      


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn 
            20                  25                  30          


Ala Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn Leu 
        35                  40                  45              


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp 
    50                  55                  60                  


Ala Asn Ser Ser Glu Asn Gln Leu Gly Ala Gly Ala Phe Gly Gly Tyr 
65                  70                  75                  80  


Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly 
                85                  90                  95      


Arg Met Pro Tyr Lys Ala Gln Gly Val Gln Leu Thr Ala Lys Leu Gly 
            100                 105                 110         


Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr Thr Arg Leu Gly Gly Met 
        115                 120                 125             


Val Trp Arg Ala Asp Thr Gly Val Ser Pro Val Phe Ala Gly Gly Val 
    130                 135                 140                 


Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu Glu Tyr Gln Trp 
145                 150                 155                 160 


Thr Asn Asn Ile Gly Asp Asn Gly Met Leu Ser Leu Gly Val Ser Tyr 
                165                 170                 175     


Arg Phe Gly Pro Ile Thr Gly Asp Thr Trp Tyr Thr Gly Ala Lys Leu 
            180                 185                 190         


Gly Trp Ser Gln Tyr His His His His His His 
        195                 200             


<210>  11
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMP154 OMP0 with short loops and truncated C-term,  but loop 1 
       elongated

<400>  11
atgcatcacc atcaccatca ctcgagcggt gctccgaaag ataacacctg gtacactggt       60

gctaaactgg gctggtccca gtaccatgac actggtttca tcaacaacac ccctacccca      120

acgccgacgc ctactccgca acagtcgacc aaccaacccg ggacgaatca acagccgacg      180

ccgacgccaa ccccaacccc aaatggcccg acccatgaaa accaactggg cgctggtgct      240

tttggtggtt accaggttaa cccgtatgtt ggctttgaaa tgggttacga ctggttaggt      300

cgtatgccgt acaaagctca gggcgttcaa ctgaccgcta aactgggtta cccaatcact      360

gacgacctgg acatctacac tcgtctgggt ggcatggtat ggcgtgcaga cactggcgtt      420

tctccggtct tcgctggcgg tgttgagtac gcgatcactc ctgaaatcgc tacccgtctg      480

gaataccagt ggaccaacaa catcaacggc atgctgagcc tgggtgtttc ctaccgtttc      540

ggtcagtaaa cgcgtgagga attttgaaga tccggctgct aacaaagccc gaaaggaagc      600


<210>  12
<211>  182
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP154 OMP0 with short loops and truncated C-term, but loop 1 
       elongated.

<400>  12

Met His His His His His His Ser Ser Gly Ala Pro Lys Asp Asn Thr 
1               5                   10                  15      


Trp Tyr Thr Gly Ala Lys Leu Gly Trp Ser Gln Tyr His Asp Thr Gly 
            20                  25                  30          


Phe Ile Asn Asn Thr Pro Thr Pro Thr Pro Thr Pro Thr Pro Gln Gln 
        35                  40                  45              


Ser Thr Asn Gln Pro Gly Thr Asn Gln Gln Pro Thr Pro Thr Pro Thr 
    50                  55                  60                  


Pro Thr Pro Asn Gly Pro Thr His Glu Asn Gln Leu Gly Ala Gly Ala 
65                  70                  75                  80  


Phe Gly Gly Tyr Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly Tyr 
                85                  90                  95      


Asp Trp Leu Gly Arg Met Pro Tyr Lys Ala Gln Gly Val Gln Leu Thr 
            100                 105                 110         


Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr Thr Arg 
        115                 120                 125             


Leu Gly Gly Met Val Trp Arg Ala Asp Thr Gly Val Ser Pro Val Phe 
    130                 135                 140                 


Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu 
145                 150                 155                 160 


Glu Tyr Gln Trp Thr Asn Asn Ile Asn Gly Met Leu Ser Leu Gly Val 
                165                 170                 175     


Ser Tyr Arg Phe Gly Gln 
            180         


<210>  13
<211>  426
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMP170 short loops, n-terminal his tag, cysteine at aa 122

<400>  13
atgcatcacc atcaccatca ctcgagtgaa aaccaactgg gcgctggtgc ttttggtggt       60

taccaggtta acccgtatgt tggctttgaa atgggttacg actggttagg tcgtatgccg      120

tacaaagctc agggcgttca actgaccgct aaactgggtt acccaatcac tgacgacctg      180

gacatctaca ctcgtttggg tggcatggta tggcgtgcag acactggcgt ttctccggtc      240

ttcgctggcg gtgttgagta cgcgatcact cctgaaatcg ctacccgtct ggaataccag      300

tggaccaaca acatcggtga caacggcatg ctgagcctgg gtgtttccta ccgtttcggt      360

ccgtgtacag gtgatacctg gtacactggt gctaaactgg gctggtccca gtacaaccag      420

tgataa                                                                 426


<210>  14
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP170 short loops, n-terminal his tag, cysteine at aa 122

<400>  14

Met His His His His His His Ser Ser Glu Asn Gln Leu Gly Ala Gly 
1               5                   10                  15      


Ala Phe Gly Gly Tyr Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly 
            20                  25                  30          


Tyr Asp Trp Leu Gly Arg Met Pro Tyr Lys Ala Gln Gly Val Gln Leu 
        35                  40                  45              


Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp Ile Tyr Thr 
    50                  55                  60                  


Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Gly Val Ser Pro Val 
65                  70                  75                  80  


Phe Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg 
                85                  90                  95      


Leu Glu Tyr Gln Trp Thr Asn Asn Ile Gly Asp Asn Gly Met Leu Ser 
            100                 105                 110         


Leu Gly Val Ser Tyr Arg Phe Gly Pro Cys Thr Gly Asp Thr Trp Tyr 
        115                 120                 125             


Thr Gly Ala Lys Leu Gly Trp Ser Gln Tyr Asn Gln 
    130                 135                 140 


<210>  15
<211>  627
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OMP171, as 170 but with alpha helical spacer after his tag.

<400>  15
atgcatcacc atcaccatca ctcgagcgta gacaacaaat tcaacaaaga aaaaaaaaac       60

gcattctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc      120

caaagtttaa aagcggcccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta      180

aatgatgctc aggcgccgaa agtagacgcg aattcgagca cgtcgagtga aaaccaactg      240

ggcgctggtg cttttggtgg ttaccaggtt aacccgtatg ttggctttga aatgggttac      300

gactggttag gtcgtatgcc gtacaaagct cagggcgttc aactgaccgc taaactgggt      360

tacccaatca ctgacgacct ggacatctac actcgtttgg gtggcatggt atggcgtgca      420

gacactggcg tttctccggt cttcgctggc ggtgttgagt acgcgatcac tcctgaaatc      480

gctacccgtc tggaatacca gtggaccaac aacatcggtg acaacggcat gctgagcctg      540

ggtgtttcct accgtttcgg tccgtgtaca ggtgatacct ggtacactgg tgctaaactg      600

ggctggtccc agtacaacca gtgataa                                          627


<210>  16
<211>  207
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OMP171, as 170 but with alpha helical spacer after his tag

<400>  16

Met His His His His His His Ser Ser Val Asp Asn Lys Phe Asn Lys 
1               5                   10                  15      


Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn 
            20                  25                  30          


Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser 
        35                  40                  45              


Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
    50                  55                  60                  


Ala Pro Lys Val Asp Ala Asn Ser Ser Thr Ser Ser Glu Asn Gln Leu 
65                  70                  75                  80  


Gly Ala Gly Ala Phe Gly Gly Tyr Gln Val Asn Pro Tyr Val Gly Phe 
                85                  90                  95      


Glu Met Gly Tyr Asp Trp Leu Gly Arg Met Pro Tyr Lys Ala Gln Gly 
            100                 105                 110         


Val Gln Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu Asp 
        115                 120                 125             


Ile Tyr Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Gly Val 
    130                 135                 140                 


Ser Pro Val Phe Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu Ile 
145                 150                 155                 160 


Ala Thr Arg Leu Glu Tyr Gln Trp Thr Asn Asn Ile Gly Asp Asn Gly 
                165                 170                 175     


Met Leu Ser Leu Gly Val Ser Tyr Arg Phe Gly Pro Cys Thr Gly Asp 
            180                 185                 190         


Thr Trp Tyr Thr Gly Ala Lys Leu Gly Trp Ser Gln Tyr Asn Gln 
        195                 200                 205         


