                         SEQUENCE LISTING

<110>  DSM IP ASSETS B.V.
       METZ, James G.
       KUNER, Jerry M.
       McCASKILL, David G.
       FOSTER, Mendy L.
 
<120>  FACTORS FOR THE PRODUCTION AND ACCUMULATION OF POLYUNSATURATED 
       FATTY ACIDS (PUFAs) DERIVED FROM PUFA SYNTHASES

<130>  29324-WO-PCT

<140>  TBD
<141>  2015-01-28

<150>  61/932,310
<151>  2014-01-28

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  137
<212>  PRT
<213>  Schizochytrium sp

<400>  1

Met Thr Ala Gln Gly Gly Tyr Arg Ser Glu Met Leu Met Tyr Tyr Glu 
1               5                   10                  15      


Asp Thr Asp Leu Thr Gly Ala Val Tyr Ala Gly Asn Tyr Phe Lys Tyr 
            20                  25                  30          


Phe Glu Arg Ala Arg Asp Glu Ala Val Gly Ile Asp Val Leu Lys Thr 
        35                  40                  45              


Leu Met Asp Lys Glu Gly Leu Ala Leu Tyr Val Arg Lys Met Gly Glu 
    50                  55                  60                  


Met Thr Phe Lys Gly Gly Ala Lys His Ala Asp Thr Leu Val Val Glu 
65                  70                  75                  80  


Ser Ser Val Glu Ala Pro Ser Asp Phe Arg Leu Val Phe Lys Gln Arg 
                85                  90                  95      


Ala Ser Val Lys Asp Arg Pro Glu Thr Ile Ile Val Glu Thr Asp Val 
            100                 105                 110         


Glu Val Val Cys Ile Asp Met Lys Thr Gln Arg Val Ala Lys Ile Pro 
        115                 120                 125             


Thr Gln Ile Arg Glu Ala Leu Arg Ile 
    130                 135         


<210>  2
<211>  321
<212>  PRT
<213>  Schizochytrium sp

<400>  2

Met Ala Ala Pro Ser Thr Ala Val Cys Gly Glu Leu Pro Lys Leu Asp 
1               5                   10                  15      


Glu Ala Pro Leu Lys Val Ser Arg Ala Arg Gly Tyr Asp Ala Leu Asp 
            20                  25                  30          


Val Lys Val Tyr Arg Glu Asp Thr Asp Thr Val Gly Ile Val Phe Tyr 
        35                  40                  45              


Arg Asn Phe Leu Thr Trp Phe Glu Arg Gly Arg Glu Asn Ala Ile Ser 
    50                  55                  60                  


Thr Asp Phe Leu Ala Gly Leu Phe Glu Tyr Ser Gly Asp Ser Phe Val 
65                  70                  75                  80  


Val Thr Arg Ser Glu Gln Ser Phe Arg Lys Pro Ala Phe Tyr Gly Asp 
                85                  90                  95      


Glu Leu Glu Val Arg Thr Ile Pro Phe Ala Asp Gly Pro Phe Arg Leu 
            100                 105                 110         


His Phe Asp Gln Ser Ile Trp Arg Lys Ser Asp Asn Thr Leu Leu Val 
        115                 120                 125             


Ala Gly Phe Val Glu Met Val Thr Val Ser Arg Thr Phe Gln Leu Thr 
    130                 135                 140                 


Lys Val Pro Gln Pro Val His Asp Leu Ile Tyr Tyr Phe Asp Asp Cys 
145                 150                 155                 160 


Lys Ser Asn Phe Thr Tyr Cys Glu Lys Pro Gly Ala Lys Lys Arg Pro 
                165                 170                 175     


Leu Arg Arg Lys Pro Gly Ala Pro Ser Ser Leu Gly Lys Thr Thr Glu 
            180                 185                 190         


Leu Asp Leu Val Ile His Leu Ala Asp Thr Asp Phe Thr Gly Ile Ala 
        195                 200                 205             


Phe His Pro Asn Tyr Tyr Cys Trp Phe Glu Arg Ala Arg Ser Asp Phe 
    210                 215                 220                 


Leu Ser Asn Glu Ile Leu Ala Arg Ala Lys Thr Glu Phe His Ala Val 
225                 230                 235                 240 


Pro Val Val Arg Ser Ala Lys Leu Ala Tyr Lys Asn Gly Ala Arg Pro 
                245                 250                 255     


Val Glu Pro Leu Arg Ile Thr Thr Thr Gln Asp Pro Lys Gly Glu His 
            260                 265                 270         


Ser Asp Phe Val Val Pro Ile Leu Gln Lys Leu Thr Arg Val Ser Asn 
        275                 280                 285             


Asp Gln Thr Leu Val Glu Ala Val Phe Glu Met Cys Phe Val His Asp 
    290                 295                 300                 


Lys Glu Arg His Leu Val Lys Val Pro Ser Ile Val Arg Asp Ala Ile 
305                 310                 315                 320 


Ala 
    


<210>  3
<211>  156
<212>  PRT
<213>  Schizochytrium sp

<400>  3

Met Val Met Val Ala Glu Glu Lys Arg Ala His Glu Val Ala Val Gln 
1               5                   10                  15      


Leu Tyr Tyr Glu Asp Thr Asp Phe Ser Gly Phe Val His His Ala Asn 
            20                  25                  30          


Phe Leu Arg Tyr Phe Glu Arg Gly Arg Asp Glu Met Ile Gly Leu Pro 
        35                  40                  45              


Val Leu Lys Cys Leu Ala Gln Asp Asp Ser Ser Ser Ser Ser Ser Ala 
    50                  55                  60                  


Thr Ser Ile Gly Gly Gly Glu Pro Pro Val Ser Leu Phe Val His Lys 
65                  70                  75                  80  


Val His Glu Leu Ser Phe Lys Gly Arg Ala Arg His Gly Glu Met Leu 
                85                  90                  95      


Val Val Arg Ser Arg Val Val Lys Glu Ser Asp Phe Arg Leu Arg Phe 
            100                 105                 110         


Ala His Glu Ala Trp Val Gly Asn Thr Leu Val Ala Ser Gly Ser Met 
        115                 120                 125             


Asp Val Val Phe Leu Cys Gly Ser Val Asp Ala Arg Leu Val Lys Ile 
    130                 135                 140                 


Pro Asn Ser Val Asp Val Ala Leu His Gly Tyr Tyr 
145                 150                 155     


<210>  4
<211>  321
<212>  PRT
<213>  Schizochytrium sp

<400>  4

Met Arg Ile Asp Glu Glu Ala Ile Arg Val Ala Ala Ala Arg Gly Tyr 
1               5                   10                  15      


Asp Ala Leu Pro Val Thr Val Tyr Arg Glu Phe Thr Asp Cys Leu Gly 
            20                  25                  30          


Ile Val Phe Tyr Arg His Tyr Leu Ala Trp Phe Glu Arg Gly Arg Glu 
        35                  40                  45              


Asn Val Ile Ser Val Gln Phe Leu Ala Asp Leu Phe Arg Glu Thr Gly 
    50                  55                  60                  


Glu Ser Phe Val Val Thr Arg Ser Glu Gln Val Phe Lys Arg Ser Ala 
65                  70                  75                  80  


Arg Tyr Gly Asp Gln Leu Glu Val Arg Thr Ile Pro Phe Leu Asp Gly 
                85                  90                  95      


Asp Tyr Arg Leu Gly Phe Asp Gln Ser Val Trp His Gly Asn Glu Met 
            100                 105                 110         


Leu Val His Gly Phe Val Glu Met Val Cys Val Ser Lys Ser Phe Gln 
        115                 120                 125             


Leu Ala Gln Gln Pro Ala Leu Val Arg Lys Leu Ile Gly Cys Phe Asp 
    130                 135                 140                 


Glu Cys Thr Arg Asn Phe Thr Tyr Val Gly Thr Lys Ala Arg Met Pro 
145                 150                 155                 160 


Gln Thr Ile Arg Arg Arg Arg Gly Thr Ala Ser Leu Pro Gln Ala Gln 
                165                 170                 175     


Lys Pro Leu Val Phe Asp Gly Leu His Leu His Gln Ala Asp Thr Asp 
            180                 185                 190         


Phe Thr Gly Ile Thr Phe His Pro Asn Tyr Tyr Cys Tyr Phe Glu Arg 
        195                 200                 205             


Ala Arg Ser Gln Ala Leu Thr Pro Ala Val Leu Ala Asn Val Ala Glu 
    210                 215                 220                 


Phe Ala Asn Ala Val Pro Val Ile Arg Gln Ala Arg Met Thr Phe Lys 
225                 230                 235                 240 


Gln Gly Ala Arg Ala Tyr Glu Thr Leu Arg Val Leu Thr Ser Ile Ala 
                245                 250                 255     


Leu Asp Asp Ser Gly Gly Ser Ser Ser Ser Ser Asn Lys Tyr Val Val 
            260                 265                 270         


Pro Phe Glu Gln Val Leu Val Arg Arg Glu Asp Asp Lys Val Leu Val 
        275                 280                 285             


Glu Ala Arg Ile Glu Ile Val Phe Val Asp Gln Thr Thr Lys Leu Pro 
    290                 295                 300                 


Cys Pro Ile Pro Asp Ala Val Ala Ala Lys Met Gln Glu Leu Phe Ala 
305                 310                 315                 320 


Val 
    


<210>  5
<211>  411
<212>  DNA
<213>  Schizochytrium sp

<400>  5
atgacggcgc agggcggcta cagatcggag atgctcatgt actatgagga cacggacctg       60

accggagccg tctatgcggg caactacttc aagtactttg agcgcgcgcg cgacgaggct      120

gtgggcatcg atgtcctcaa gacgctcatg gacaaggagg gcctggcttt gtacgtgcgc      180

aaaatgggcg agatgacctt taaaggaggc gccaagcacg ccgacacgct cgtcgtcgag      240

tcctctgtcg aggctccctc ggactttcgc cttgtgttca agcagcgggc atccgtcaag      300

gaccgtcccg agacgatcat tgtcgagacc gatgttgagg tcgtttgcat cgacatgaaa      360

acgcagcgtg tcgccaagat cccgacgcaa atccgggaag cacttcgtat c               411


<210>  6
<211>  963
<212>  DNA
<213>  Schizochytrium sp

<400>  6
atggctgcgc catcgactgc agtctgcggc gagctgccaa agctcgacga ggcgcctctc       60

aaggtgtctc gtgcacgtgg ctacgacgcg ctcgacgtca aggtgtacag agaggacaca      120

gacacagtag ggatcgtgtt ctatcgtaac tttttgacct ggtttgagcg tggccgggaa      180

aacgcgatct ccacagactt tctcgcagga ctgttcgagt acagtggtga ctccttcgtg      240

gtcacgcggt ccgagcagtc gtttcgcaag cctgcatttt acggcgatga actcgaagtc      300

cgaaccattc cttttgcaga tgggcccttt cgcctgcact ttgaccagag catctggcga      360

aagagcgaca acacattgct agtcgctggc tttgtagaga tggtcacggt gagcagaact      420

tttcagctca ccaaggtacc tcagccggtg cacgacctca tttattactt tgacgattgc      480

aagtcgaact tcacctactg cgaaaagccc ggcgccaaga aaaggccgct tcggcgtaag      540

cccggggcgc cctcttcact tggcaaaacc acagagcttg acctggtcat tcacttggcc      600

gacactgact ttactggaat cgcattccac cccaactact actgttggtt cgagcgtgcg      660

cgctcggatt ttctcagcaa tgagattctt gcacgcgcca agaccgagtt tcatgctgtt      720

cccgttgtgc gcagtgcaaa actcgcgtac aaaaacggcg cgaggcctgt tgagccgctc      780

cgcattacaa cgacgcaaga tccgaagggc gagcactcgg actttgtcgt accgattctt      840

caaaagctta cgcgtgtctc gaacgaccag acgctcgtcg aagccgtctt tgagatgtgc      900

tttgttcatg acaaggagcg ccacctcgtc aaggtcccgt cgatcgttcg cgatgctatt      960

gcg                                                                    963


<210>  7
<211>  468
<212>  DNA
<213>  Schizochytrium sp

<400>  7
atggtcatgg tcgcggagga gaagagggcg cacgaggtgg cagtacagtt gtactatgag       60

gacacggact tctccggctt tgtccatcat gccaacttcc tgcgctactt tgaacgcggc      120

cgggatgaga tgattggcct gcccgttctc aaatgcttgg cccaagacga tagctcttct      180

tcttcttctg caacttcaat tggtggtggc gagcctccag tatcattgtt cgtgcataag      240

gtgcacgagt tgtcgttcaa aggtcgcgct cggcacggtg agatgctcgt ggtgcggtca      300

cgagtggtca aggaatcgga cttccgactg cgctttgcac acgaagcgtg ggtggggaac      360

acgctcgtgg cctctggatc aatggacgtg gtgttcctgt gtggctcggt cgatgcgcga      420

ttagtgaaga tccctaactc ggtcgatgtg gccttgcacg gatactat                   468


<210>  8
<211>  963
<212>  DNA
<213>  Schizochytrium sp

<400>  8
atgagaatcg acgaggaggc gatacgcgtg gcagcggcgc gcgggtacga cgccttgccc       60

gtgacagtgt atcgagagtt taccgactgc ctgggcattg tgttctaccg gcactaccta      120

gcgtggtttg agcgcgggcg cgagaacgtc atctcggtgc agttcttggc ggatctgttt      180

cgcgaaacgg gggagtcgtt cgtggtgacg cgctccgagc aagtgtttaa gcgctcagcg      240

cgctatggcg accaactcga agtgcgcacc attcctttcc tggacggcga ctaccgcctc      300

ggcttcgacc agagcgtgtg gcacggcaat gagatgctcg tgcatggctt cgtggagatg      360

gtctgcgtaa gcaagagctt ccagctggcg caacaaccgg cgctcgtgcg caagctgatc      420

ggctgctttg acgagtgcac gcgcaacttc acctacgtcg gcaccaaggc ccgcatgccc      480

caaaccattc gacgacgcag aggcacggcc agtctaccac aagcacagaa gcctctagtg      540

tttgacgggc tgcacttgca ccaagcggac acagacttca caggtatcac ttttcacccc      600

aactactact gctactttga acgcgcgcgc tcgcaggcat tgactcccgc cgtattagcg      660

aacgtggctg agttcgccaa cgctgtgcca gtcatccgcc aagcccgcat gaccttcaag      720

caaggcgcga gagcgtacga gacactccgc gtgctcacat caattgctct ggatgatagc      780

ggcggcagca gcagcagcag caacaagtat gtcgtgccgt ttgagcaggt gctcgtgcga      840

agagaagacg acaaggtgct ggtggaggcg cgaatcgaga ttgtctttgt ggaccagact      900

acgaagttgc cctgcccgat tcctgacgca gtggcagcca agatgcagga gttgtttgcg      960

gta                                                                    963


