                               SEQUENCE LISTING

<110> CURIS, INC.
      GENENTECH, INC.
 
<120> G-PROTEIN COUPLED RECEPTORS IN HEDGEHOG SIGNALING

<130> CIBT-224-WO1

<140>
<141>

<150> 61/934,620
<151> 2014-01-31

<160> 48    

<170> PatentIn version 3.5

<210> 1
<211> 373
<212> PRT
<213> Homo sapiens

<220>
<221> source
<223> /note="Human TPRA40 amino acid sequence"

<300>
<308> GenBank Accession No. AAH50711.1

<400> 1
Met Asp Thr Leu Glu Glu Val Thr Trp Ala Asn Gly Ser Thr Ala Leu 
1               5                   10                  15      


Pro Pro Pro Leu Ala Pro Asn Ile Ser Val Pro His Arg Cys Leu Leu 
            20                  25                  30          


Leu Leu Tyr Glu Asp Ile Gly Thr Ser Arg Val Arg Tyr Trp Asp Leu 
        35                  40                  45              


Leu Leu Leu Ile Pro Asn Val Leu Phe Leu Ile Phe Leu Leu Trp Lys 
    50                  55                  60                  


Leu Pro Ser Ala Arg Ala Lys Ile Arg Ile Thr Ser Ser Pro Ile Phe 
65                  70                  75                  80  


Ile Thr Phe Tyr Ile Leu Val Phe Val Val Ala Leu Val Gly Ile Ala 
                85                  90                  95      


Arg Ala Val Val Ser Met Thr Val Ser Thr Ser Asn Ala Ala Thr Val 
            100                 105                 110         


Ala Asp Lys Ile Leu Trp Glu Ile Thr Arg Phe Phe Leu Leu Ala Ile 
        115                 120                 125             


Glu Leu Ser Val Ile Ile Leu Gly Leu Ala Phe Gly His Leu Glu Ser 
    130                 135                 140                 


Lys Ser Ser Ile Lys Arg Val Leu Ala Ile Thr Thr Val Leu Ser Leu 
145                 150                 155                 160 


Ala Tyr Ser Val Thr Gln Gly Thr Leu Glu Ile Leu Tyr Pro Asp Ala 
                165                 170                 175     


His Leu Ser Ala Glu Asp Phe Asn Ile Tyr Gly His Gly Gly Arg Gln 
            180                 185                 190         


Phe Trp Leu Val Ser Ser Cys Phe Phe Phe Leu Val Tyr Ser Leu Val 
        195                 200                 205             


Val Ile Leu Pro Lys Thr Pro Leu Lys Glu Arg Ile Ser Leu Pro Ser 
    210                 215                 220                 


Arg Arg Ser Phe Tyr Val Tyr Ala Gly Ile Leu Ala Leu Leu Asn Leu 
225                 230                 235                 240 


Leu Gln Gly Leu Gly Ser Val Leu Leu Cys Phe Asp Ile Ile Glu Gly 
                245                 250                 255     


Leu Cys Cys Val Asp Ala Thr Thr Phe Leu Tyr Phe Ser Phe Phe Ala 
            260                 265                 270         


Pro Leu Ile Tyr Val Ala Phe Leu Arg Gly Phe Phe Gly Ser Glu Pro 
        275                 280                 285             


Lys Ile Leu Phe Ser Tyr Lys Cys Gln Val Asp Glu Thr Glu Glu Pro 
    290                 295                 300                 


Asp Val His Leu Pro Gln Pro Tyr Ala Val Ala Arg Arg Glu Gly Leu 
305                 310                 315                 320 


Glu Ala Ala Gly Ala Ala Gly Ala Ser Ala Ala Ser Tyr Ser Ser Thr 
                325                 330                 335     


Gln Phe Asp Ser Ala Gly Gly Val Ala Tyr Leu Asp Asp Ile Ala Ser 
            340                 345                 350         


Met Pro Cys His Thr Gly Ser Ile Asn Ser Thr Asp Ser Glu Arg Trp 
        355                 360                 365             


Lys Ala Ile Asn Ala 
    370             


<210> 2
<211> 369
<212> PRT
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse TPRA40 amino acid sequence"

<300>
<308> GenBank Accession No. NP_036036.2

<400> 2
Met Ala Ser Leu Gln Glu Ala Asn Gly Ser Thr Ala Trp Pro Pro Pro 
1               5                   10                  15      


Thr Ala Ser Asn Ile Ser Glu Pro His Gln Cys Leu Leu Leu Leu Tyr 
            20                  25                  30          


Glu Asp Ile Gly Ser Ser Arg Val Arg Tyr Trp Asp Leu Leu Leu Leu 
        35                  40                  45              


Ile Pro Asn Val Leu Phe Phe Ile Phe Leu Leu Trp Lys Leu Pro Leu 
    50                  55                  60                  


Ala Arg Ala Lys Ile Arg Val Thr Ser Ser Pro Ile Phe Ile Thr Phe 
65                  70                  75                  80  


Tyr Ile Leu Val Phe Val Val Ala Leu Val Gly Ile Ala Arg Ala Val 
                85                  90                  95      


Val Ser Met Thr Val Ser Ala Ser Asp Ala Ala Thr Val Ala Asp Lys 
            100                 105                 110         


Ile Leu Trp Glu Ile Thr Arg Phe Phe Leu Leu Ala Ile Glu Leu Ser 
        115                 120                 125             


Val Ile Ile Leu Gly Leu Ala Phe Gly His Leu Glu Ser Lys Ser Ser 
    130                 135                 140                 


Ile Lys Arg Val Leu Ala Ile Thr Thr Val Leu Ser Leu Ala Tyr Ser 
145                 150                 155                 160 


Val Thr Gln Gly Thr Leu Glu Ile Leu Tyr Pro Asp Ser His Leu Ser 
                165                 170                 175     


Ala Glu Asp Phe Asn Ile Tyr Gly His Gly Gly Arg Gln Phe Trp Leu 
            180                 185                 190         


Val Ser Ser Cys Phe Phe Phe Leu Val Tyr Ser Leu Val Val Ile Leu 
        195                 200                 205             


Pro Lys Thr Pro Leu Lys Glu Arg Val Ser Leu Pro Ser Arg Arg Ser 
    210                 215                 220                 


Phe Tyr Val Tyr Ala Gly Ile Leu Ala Thr Leu Asn Leu Leu Gln Gly 
225                 230                 235                 240 


Leu Gly Ser Ala Leu Leu Cys Ala Asn Ile Ile Val Gly Leu Cys Cys 
                245                 250                 255     


Val Asp Ala Thr Thr Phe Leu Tyr Phe Ser Phe Phe Ala Pro Leu Ile 
            260                 265                 270         


Tyr Val Ala Phe Leu Arg Gly Phe Phe Gly Ser Glu Pro Lys Ile Leu 
        275                 280                 285             


Phe Ser Tyr Lys Cys Gln Val Asp Glu Ala Glu Glu Pro Asp Met His 
    290                 295                 300                 


Leu Pro Gln Pro Tyr Ala Val Ala Arg Arg Glu Gly Ile Glu Ser Ala 
305                 310                 315                 320 


Gly Pro Ala Cys Ala Ser Ala Ala Asn Tyr Ser Ser Thr Gln Phe Asp 
                325                 330                 335     


Ser Ala Gly Val Ala Tyr Leu Asp Asp Ile Ala Ser Met Pro Cys His 
            340                 345                 350         


Thr Gly Ser Ile Asn Ser Thr Asp Ser Glu Arg Trp Lys Ala Ile Asn 
        355                 360                 365             


Ala 
    


<210> 3
<211> 378
<212> PRT
<213> Danio rerio

<220>
<221> source
<223> /note="Zebrafish TPRA40 amino acid sequence"

<300>
<308> GenBank Accession No. NP_001025422.1

<400> 3
Met Leu Glu Thr Val Thr Asp Val Ala Ser Phe Val His Tyr Gly Asn 
1               5                   10                  15      


Thr Ser Val Phe Pro Thr Ala Asp Asn Ser Ser Glu Ile Ile Pro Asp 
            20                  25                  30          


Gly Glu Ser Asn Ile Ser Lys Pro His Arg Cys Leu Gln Val Leu Tyr 
        35                  40                  45              


Asp Asp Ile Gly Thr Ser Arg Val Arg Tyr Trp Asp Val Met Leu Leu 
    50                  55                  60                  


Ile Pro Asn Val Ala Phe Leu Val Phe Leu Met Trp Lys Leu Pro Ser 
65                  70                  75                  80  


Ala Arg Ala Lys Ile Arg Leu Thr Ser Ser Pro Ile Phe Val Ala Phe 
                85                  90                  95      


Tyr Ile Leu Val Phe Val Val Ala Ala Val Gly Ile Thr Arg Ala Ile 
            100                 105                 110         


Val Ser Met Thr Val Ser Thr Ser Ser Ala Ala Thr Leu Ile Asp Lys 
        115                 120                 125             


Val Leu Trp Glu Ile Thr Arg Phe Phe Leu Leu Ala Ile Glu Leu Ser 
    130                 135                 140                 


Val Ile Ile Leu Gly Leu Ala Phe Gly His Leu Glu Ser Lys Ser Ser 
145                 150                 155                 160 


Ile Lys Arg Val Leu Ala Ile Thr Ala Val Leu Ser Leu Ala Tyr Ser 
                165                 170                 175     


Ile Thr Gln Gly Thr Leu Glu Ile Arg Phe Pro Asp Lys His Leu Ser 
            180                 185                 190         


Ala Lys Asp Phe Asn Ile Tyr Gly His Gly Gly Arg His Phe Trp Leu 
        195                 200                 205             


Ala Ser Ser Cys Phe Phe Phe Leu Val Tyr Ser Leu Ile Val Ile Leu 
    210                 215                 220                 


Pro Lys Thr Pro Ile Arg Glu Arg Ile Ser Leu Pro Ser Lys Arg Ser 
225                 230                 235                 240 


Phe Tyr Val Tyr Ser Gly Ile Leu Ala Leu Leu Asn Leu Val Gln Gly 
                245                 250                 255     


Leu Gly Ser Ala Leu Leu Cys Ala Asp Ile Ile Glu Gly Leu Cys Cys 
            260                 265                 270         


Val Asp Val Thr Thr Phe Leu Tyr Phe Ser Val Phe Ala Pro Leu Ile 
        275                 280                 285             


Tyr Val Thr Phe Leu Lys Gly Phe Phe Gly Ser Glu Pro Lys Ile Leu 
    290                 295                 300                 


Phe Ser Tyr Lys Ser Gln Ile Asp Glu Pro Glu Asp Ser Asp Val His 
305                 310                 315                 320 


Leu Pro His Thr Ser Ser Ser Gly Leu Gly Arg Lys Asp Leu Asp Arg 
                325                 330                 335     


Gly Ser Tyr Ser Ser Thr Gln Ile Asp Gly Ser Gly Ala Tyr Leu Asp 
            340                 345                 350         


Asp Val Val Ser Gly Pro Phe Gly Gly Ala His Ser Ile Asn Ser Val 
        355                 360                 365             


Asp Ser Asp Arg Trp Arg Ser Ile Asn Thr 
    370                 375             


<210> 4
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary murine Gli1 5' primer"

<400> 4
gcagtgggta acatgagtgt ct                                                22


<210> 5
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary murine Gli1 3' primer"

<400> 5
aggcactaga gttgaggaat tgt                                               23


<210> 6
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary murine Gli1 probe"

<400> 6
ctctccaggc agagacccca gc                                                22


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary human Gli1 5' primer"

<400> 7
cgctgcgaaa acatgtcaag                                                   20


<210> 8
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary human Gli1 3' primer"

<400> 8
ccacggtgcc gtttggt                                                      17


<210> 9
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary human Gli1 probe"

<400> 9
cagtgcatgg tcctgacgcc ca                                                22


<210> 10
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary murine TPRA40 5' primer"

<400> 10
tgcaggaggc caatggaa                                                     18


<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary murine TPRA40 3' primer"

<400> 11
gggctcactg atattggatg ct                                                22


<210> 12
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary murine TPRA40 probe"

<400> 12
acagcgtggc caccgccc                                                     18


<210> 13
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary human TPRA40 5' primer"

<400> 13
cctggtctac tctctggtgg tcat                                              24


<210> 14
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary human TPRA40 3' primer"

<400> 14
ccgagaaggc agggagatg                                                    19


<210> 15
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      exemplary human TPRA40 probe"

<400> 15
cccaagaccc cgctgaagga gc                                                22


<210> 16
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse TPRA40 siRNA #9"

<400> 16
ggaggaguuu cuacgugua                                                    19


<210> 17
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse TPRA40 siRNA #10"

<400> 17
cggcaguucu ggcucguca                                                    19


<210> 18
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse TPRA40 siRNA #11"

<400> 18
cgacaguugc ugacaagau                                                    19


<210> 19
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse TPRA40 siRNA #12"

<400> 19
gccauugagc ugaguguga                                                    19


<210> 20
<211> 19
<212> RNA
<213> Homo sapiens

<220>
<221> source
<223> /note="TPRA40 siRNA #6"

<400> 20
ggucagcucc ugcuucuuc                                                    19


<210> 21
<211> 19
<212> RNA
<213> Homo sapiens

<220>
<221> source
<223> /note="TPRA40 siRNA #7"

<400> 21
gagaguaagu ccagcauca                                                    19


<210> 22
<211> 19
<212> RNA
<213> Homo sapiens

<220>
<221> source
<223> /note="TPRA40 siRNA #8"

<400> 22
ggaggagcuu cuacgugua                                                    19


<210> 23
<211> 19
<212> RNA
<213> Homo sapiens

<220>
<221> source
<223> /note="TPRA40 siRNA #9"

<400> 23
ccacaaccuu ccuguacuu                                                    19


<210> 24
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Galpha(i)1 siRNA #1"

<400> 24
gaauagcaca gccaaauua                                                    19


<210> 25
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Galpha(i)1 siRNA #2"

<400> 25
ggaugaugcu cgccaacuu                                                    19


<210> 26
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Galpha(i)1 siRNA #3"

<400> 26
uaacagacgu caucauaaa                                                    19


<210> 27
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Galpha(i)1 siRNA #4"

<400> 27
gaagaggagu guaagcagu                                                    19


<210> 28
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Smo siRNA #1"

<400> 28
caauuggccu ggugcuuau                                                    19


<210> 29
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Smo siRNA #2"

<400> 29
gagcguagcu uccgggacu                                                    19


<210> 30
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Smo siRNA #3"

<400> 30
ggaguagucu gguucgugg                                                    19


<210> 31
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Smo siRNA #4"

<400> 31
gcuacaagaa cuaucggua                                                    19


<210> 32
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ift88 siRNA#5"

<400> 32
guagcuagcu gcuuuagaa                                                    19


<210> 33
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ift88 siRNA#6"

<400> 33
cgucagcucu cacuaauaa                                                    19


<210> 34
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ift88 siRNA#7"

<400> 34
gcuuggagcu uauuacauu                                                    19


<210> 35
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ift88 siRNA#8"

<400> 35
cggagaaugu ugaauguuu                                                    19


<210> 36
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ssna1 siRNA #1"

<400> 36
gaaccugacu aaagccaca                                                    19


<210> 37
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ssna1 siRNA #2"

<400> 37
gcaacgaguu ugaccggac                                                    19


<210> 38
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ssna1 siRNA #3"

<400> 38
ggaguugugu cagaagcga                                                    19


<210> 39
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Ssna1 siRNA #4"

<400> 39
acgaguuggu caagugcau                                                    19


<210> 40
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      TPRA40 Sense Morpholino oligonucleotide"

<400> 40
acatcggtca ccgtctccag cattc                                             25


<210> 41
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      TPRA40 Antisense Morpholino oligonucleotide"

<400> 41
acaaccgtga ccctctccac cattc                                             25


<210> 42
<211> 787
<212> PRT
<213> Homo sapiens

<220>
<221> source
<223> /note="Human smoothened amino acid sequence"

<300>
<308> GenBank Accession No. NP_005622.1

<400> 42
Met Ala Ala Ala Arg Pro Ala Arg Gly Pro Glu Leu Pro Leu Leu Gly 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Leu Gly Asp Pro Gly Arg Gly Ala Ala Ser 
            20                  25                  30          


Ser Gly Asn Ala Thr Gly Pro Gly Pro Arg Ser Ala Gly Gly Ser Ala 
        35                  40                  45              


Arg Arg Ser Ala Ala Val Thr Gly Pro Pro Pro Pro Leu Ser His Cys 
    50                  55                  60                  


Gly Arg Ala Ala Pro Cys Glu Pro Leu Arg Tyr Asn Val Cys Leu Gly 
65                  70                  75                  80  


Ser Val Leu Pro Tyr Gly Ala Thr Ser Thr Leu Leu Ala Gly Asp Ser 
                85                  90                  95      


Asp Ser Gln Glu Glu Ala His Gly Lys Leu Val Leu Trp Ser Gly Leu 
            100                 105                 110         


Arg Asn Ala Pro Arg Cys Trp Ala Val Ile Gln Pro Leu Leu Cys Ala 
        115                 120                 125             


Val Tyr Met Pro Lys Cys Glu Asn Asp Arg Val Glu Leu Pro Ser Arg 
    130                 135                 140                 


Thr Leu Cys Gln Ala Thr Arg Gly Pro Cys Ala Ile Val Glu Arg Glu 
145                 150                 155                 160 


Arg Gly Trp Pro Asp Phe Leu Arg Cys Thr Pro Asp Arg Phe Pro Glu 
                165                 170                 175     


Gly Cys Thr Asn Glu Val Gln Asn Ile Lys Phe Asn Ser Ser Gly Gln 
            180                 185                 190         


Cys Glu Val Pro Leu Val Arg Thr Asp Asn Pro Lys Ser Trp Tyr Glu 
        195                 200                 205             


Asp Val Glu Gly Cys Gly Ile Gln Cys Gln Asn Pro Leu Phe Thr Glu 
    210                 215                 220                 


Ala Glu His Gln Asp Met His Ser Tyr Ile Ala Ala Phe Gly Ala Val 
225                 230                 235                 240 


Thr Gly Leu Cys Thr Leu Phe Thr Leu Ala Thr Phe Val Ala Asp Trp 
                245                 250                 255     


Arg Asn Ser Asn Arg Tyr Pro Ala Val Ile Leu Phe Tyr Val Asn Ala 
            260                 265                 270         


Cys Phe Phe Val Gly Ser Ile Gly Trp Leu Ala Gln Phe Met Asp Gly 
        275                 280                 285             


Ala Arg Arg Glu Ile Val Cys Arg Ala Asp Gly Thr Met Arg Leu Gly 
    290                 295                 300                 


Glu Pro Thr Ser Asn Glu Thr Leu Ser Cys Val Ile Ile Phe Val Ile 
305                 310                 315                 320 


Val Tyr Tyr Ala Leu Met Ala Gly Val Val Trp Phe Val Val Leu Thr 
                325                 330                 335     


Tyr Ala Trp His Thr Ser Phe Lys Ala Leu Gly Thr Thr Tyr Gln Pro 
            340                 345                 350         


Leu Ser Gly Lys Thr Ser Tyr Phe His Leu Leu Thr Trp Ser Leu Pro 
        355                 360                 365             


Phe Val Leu Thr Val Ala Ile Leu Ala Val Ala Gln Val Asp Gly Asp 
    370                 375                 380                 


Ser Val Ser Gly Ile Cys Phe Val Gly Tyr Lys Asn Tyr Arg Tyr Arg 
385                 390                 395                 400 


Ala Gly Phe Val Leu Ala Pro Ile Gly Leu Val Leu Ile Val Gly Gly 
                405                 410                 415     


Tyr Phe Leu Ile Arg Gly Val Met Thr Leu Phe Ser Ile Lys Ser Asn 
            420                 425                 430         


His Pro Gly Leu Leu Ser Glu Lys Ala Ala Ser Lys Ile Asn Glu Thr 
        435                 440                 445             


Met Leu Arg Leu Gly Ile Phe Gly Phe Leu Ala Phe Gly Phe Val Leu 
    450                 455                 460                 


Ile Thr Phe Ser Cys His Phe Tyr Asp Phe Phe Asn Gln Ala Glu Trp 
465                 470                 475                 480 


Glu Arg Ser Phe Arg Asp Tyr Val Leu Cys Gln Ala Asn Val Thr Ile 
                485                 490                 495     


Gly Leu Pro Thr Lys Gln Pro Ile Pro Asp Cys Glu Ile Lys Asn Arg 
            500                 505                 510         


Pro Ser Leu Leu Val Glu Lys Ile Asn Leu Phe Ala Met Phe Gly Thr 
        515                 520                 525             


Gly Ile Ala Met Ser Thr Trp Val Trp Thr Lys Ala Thr Leu Leu Ile 
    530                 535                 540                 


Trp Arg Arg Thr Trp Cys Arg Leu Thr Gly Gln Ser Asp Asp Glu Pro 
545                 550                 555                 560 


Lys Arg Ile Lys Lys Ser Lys Met Ile Ala Lys Ala Phe Ser Lys Arg 
                565                 570                 575     


His Glu Leu Leu Gln Asn Pro Gly Gln Glu Leu Ser Phe Ser Met His 
            580                 585                 590         


Thr Val Ser His Asp Gly Pro Val Ala Gly Leu Ala Phe Asp Leu Asn 
        595                 600                 605             


Glu Pro Ser Ala Asp Val Ser Ser Ala Trp Ala Gln His Val Thr Lys 
    610                 615                 620                 


Met Val Ala Arg Arg Gly Ala Ile Leu Pro Gln Asp Ile Ser Val Thr 
625                 630                 635                 640 


Pro Val Ala Thr Pro Val Pro Pro Glu Glu Gln Ala Asn Leu Trp Leu 
                645                 650                 655     


Val Glu Ala Glu Ile Ser Pro Glu Leu Gln Lys Arg Leu Gly Arg Lys 
            660                 665                 670         


Lys Lys Arg Arg Lys Arg Lys Lys Glu Val Cys Pro Leu Ala Pro Pro 
        675                 680                 685             


Pro Glu Leu His Pro Pro Ala Pro Ala Pro Ser Thr Ile Pro Arg Leu 
    690                 695                 700                 


Pro Gln Leu Pro Arg Gln Lys Cys Leu Val Ala Ala Gly Ala Trp Gly 
705                 710                 715                 720 


Ala Gly Asp Ser Cys Arg Gln Gly Ala Trp Thr Leu Val Ser Asn Pro 
                725                 730                 735     


Phe Cys Pro Glu Pro Ser Pro Pro Gln Asp Pro Phe Leu Pro Ser Ala 
            740                 745                 750         


Pro Ala Pro Val Ala Trp Ala His Gly Arg Arg Gln Gly Leu Gly Pro 
        755                 760                 765             


Ile His Ser Arg Thr Asn Leu Met Asp Thr Glu Leu Met Asp Ala Asp 
    770                 775                 780                 


Ser Asp Phe 
785         


<210> 43
<211> 484
<212> PRT
<213> Homo sapiens

<220>
<221> source
<223> /note="Human suppressor of Fused (SuFu) amino acid sequence"

<300> 
<308> GenBank Accession No. NM_016169.2

<400> 43
Met Ala Glu Leu Arg Pro Ser Gly Ala Pro Gly Pro Thr Ala Pro Pro 
1               5                   10                  15      


Ala Pro Gly Pro Thr Ala Pro Pro Ala Phe Ala Ser Leu Phe Pro Pro 
            20                  25                  30          


Gly Leu His Ala Ile Tyr Gly Glu Cys Arg Arg Leu Tyr Pro Asp Gln 
        35                  40                  45              


Pro Asn Pro Leu Gln Val Thr Ala Ile Val Lys Tyr Trp Leu Gly Gly 
    50                  55                  60                  


Pro Asp Pro Leu Asp Tyr Val Ser Met Tyr Arg Asn Val Gly Ser Pro 
65                  70                  75                  80  


Ser Ala Asn Ile Pro Glu His Trp His Tyr Ile Ser Phe Gly Leu Ser 
                85                  90                  95      


Asp Leu Tyr Gly Asp Asn Arg Val His Glu Phe Thr Gly Thr Asp Gly 
            100                 105                 110         


Pro Ser Gly Phe Gly Phe Glu Leu Thr Phe Arg Leu Lys Arg Glu Thr 
        115                 120                 125             


Gly Glu Ser Ala Pro Pro Thr Trp Pro Ala Glu Leu Met Gln Gly Leu 
    130                 135                 140                 


Ala Arg Tyr Val Phe Gln Ser Glu Asn Thr Phe Cys Ser Gly Asp His 
145                 150                 155                 160 


Val Ser Trp His Ser Pro Leu Asp Asn Ser Glu Ser Arg Ile Gln His 
                165                 170                 175     


Met Leu Leu Thr Glu Asp Pro Gln Met Gln Pro Val Gln Thr Pro Phe 
            180                 185                 190         


Gly Val Val Thr Phe Leu Gln Ile Val Gly Val Cys Thr Glu Glu Leu 
        195                 200                 205             


His Ser Ala Gln Gln Trp Asn Gly Gln Gly Ile Leu Glu Leu Leu Arg 
    210                 215                 220                 


Thr Val Pro Ile Ala Gly Gly Pro Trp Leu Ile Thr Asp Met Arg Arg 
225                 230                 235                 240 


Gly Glu Thr Ile Phe Glu Ile Asp Pro His Leu Gln Glu Arg Val Asp 
                245                 250                 255     


Lys Gly Ile Glu Thr Asp Gly Ser Asn Leu Ser Gly Val Ser Ala Lys 
            260                 265                 270         


Cys Ala Trp Asp Asp Leu Ser Arg Pro Pro Glu Asp Asp Glu Asp Ser 
        275                 280                 285             


Arg Ser Ile Cys Ile Gly Thr Gln Pro Arg Arg Leu Ser Gly Lys Asp 
    290                 295                 300                 


Thr Glu Gln Ile Arg Glu Thr Leu Arg Arg Gly Leu Glu Ile Asn Ser 
305                 310                 315                 320 


Lys Pro Val Leu Pro Pro Ile Asn Pro Gln Arg Gln Asn Gly Leu Ala 
                325                 330                 335     


His Asp Arg Ala Pro Ser Arg Lys Asp Ser Leu Glu Ser Asp Ser Ser 
            340                 345                 350         


Thr Ala Ile Ile Pro His Glu Leu Ile Arg Thr Arg Gln Leu Glu Ser 
        355                 360                 365             


Val His Leu Lys Phe Asn Gln Glu Ser Gly Ala Leu Ile Pro Leu Cys 
    370                 375                 380                 


Leu Arg Gly Arg Leu Leu His Gly Arg His Phe Thr Tyr Lys Ser Ile 
385                 390                 395                 400 


Thr Gly Asp Met Ala Ile Thr Phe Val Ser Thr Gly Val Glu Gly Ala 
                405                 410                 415     


Phe Ala Thr Glu Glu His Pro Tyr Ala Ala His Gly Pro Trp Leu Gln 
            420                 425                 430         


Ile Leu Leu Thr Glu Glu Phe Val Glu Lys Met Leu Glu Asp Leu Glu 
        435                 440                 445             


Asp Leu Thr Ser Pro Glu Glu Phe Lys Leu Pro Lys Glu Tyr Ser Trp 
    450                 455                 460                 


Pro Glu Lys Lys Leu Lys Val Ser Ile Leu Pro Asp Val Val Phe Asp 
465                 470                 475                 480 


Ser Pro Leu His 
                


<210> 44
<211> 4898
<212> DNA
<213> Homo sapiens

<220>
<221> source
<223> /note="Human suppressor of Fused (SuFu) cDNA sequence"

<300>
<308> GenBank Accession No. NM_016169.2

<400> 44
cgccgtgcgc aggcgcggag ctagacctcg ctgcagcccc catcgcctcg gggagtctca       60

cccaccgagt ccgcccgctg gcccgtcagt gctctccccg tcgtttgccc tctccagttc      120

ccccagtgcc tgccctacgc accccgatgg cggagctgcg gcctagcggc gcccccggcc      180

ccaccgcgcc cccggcccct ggcccgactg cccccccggc cttcgcttcg ctctttcccc      240

cgggactgca cgccatctac ggagagtgcc gccgccttta ccctgaccag ccgaacccgc      300

tccaggttac cgctatcgtc aagtactggt tgggtggccc agaccccttg gactatgtta      360

gcatgtacag gaatgtgggg agcccttctg ctaacatccc cgagcactgg cactacatca      420

gcttcggcct gagtgatctc tatggtgaca acagagtcca tgagtttaca ggaacagatg      480

gacctagtgg ttttggcttt gagttgacct ttcgtctgaa gagagaaact ggggagtctg      540

ccccaccaac atggcccgca gagttaatgc agggcttggc acgatacgtg ttccagtcag      600

agaacacctt ctgcagtggg gaccatgtgt cctggcacag ccctttggat aacagtgagt      660

caagaattca gcacatgctg ctgacagagg acccacagat gcagcccgtg cagacaccct      720

ttggggtagt taccttcctc cagatcgttg gtgtctgcac tgaagagcta cactcagccc      780

agcagtggaa cgggcagggc atcctggagc tgctgcggac agtgcctatt gctggcggcc      840

cctggctgat aactgacatg cggaggggag agaccatatt tgagatcgat ccacacctgc      900

aagagagagt tgacaaaggc atcgagacag atggctccaa cctgagtggt gtcagtgcca      960

agtgtgcctg ggatgacctg agccggcccc ccgaggatga cgaggacagc cggagcatct     1020

gcatcggcac acagccccgg cgactctctg gcaaagacac agagcagatc cgggagaccc     1080

tgaggagagg actcgagatc aacagcaaac ctgtccttcc accaatcaac cctcagcggc     1140

agaatggcct cgcccacgac cgggccccga gccgcaaaga cagcctggaa agtgacagct     1200

ccacggccat cattccccat gagctgattc gcacgcggca gcttgagagc gtacatctga     1260

aattcaacca ggagtccgga gccctcattc ctctctgcct aaggggcagg ctcctgcatg     1320

gacggcactt tacatataaa agtatcacag gtgacatggc catcacgttt gtctccacgg     1380

gagtggaagg cgcctttgcc actgaggagc atccttacgc ggctcatgga ccctggttac     1440

aaattctgtt gaccgaagag tttgtagaga aaatgttgga ggatttagaa gatttgactt     1500

ctccagagga attcaaactt cccaaagagt acagctggcc tgaaaagaag ctgaaggtct     1560

ccatcctgcc tgacgtggtg ttcgacagtc cgctacacta gcctgggctg ggccctgcag     1620

gggccagcag ggagcccagc tgctccccag tgacttccag tgtaacagtt gtgtcaacga     1680

gatctccaca aataaaagga caagtgtgag gaagactgcg cagtgccacc ccgcagccca     1740

gtggggtgcc atgcacaggc cacaggccct ccacctcacc tccagctcag gggccgcacc     1800

ccgccgctgg ctaagccttg tgacccatca ggccagtgag tgggcaaatg cggaccctcc     1860

ctgcctgcag cctgcacaga ttctggtttg aggtttgact ctggaccctg gctgtgcccc     1920

taggtggaga cagccctctt tctcacctac cccctgccgc acagcccagc aggagggagg     1980

cggacagcca gatgcagagc gagtggatgc acttcccagc tcatctctgg aagcctttgc     2040

tactcaagct cctctggccg cggaacaatt cctctgatca tgtttggttt tcttcttcct     2100

tattttattt tgtagaaacc gggtggtatt ttattgctct gcaaagatgt ccagaagcca     2160

tgtatataat gttttttaaa cagaacttca ttccccgttg aactttcgca ttctctgaca     2220

gaggcctagg gctgtatctc tccctgggct gccaccagag aaggtgcttg gtgttcgcct     2280

gccagcccag agccctggag gagccggctg cacagagagg cttttcttcc cagctgggcc     2340

tgatggagcc cggggcaggg ggagagtaga gacactccct tgtgcagctt tgagcctagt     2400

ttagctgggg ccagggaggg gtgctactgt tttccaagtg aatgggtctc aaagacttgg     2460

tgaccccagc ctcatcttct aggccttttc catccaacca ggcctacctg ggagagggtg     2520

aggttcagca catcacacac catccccact gtcattcagg gcctgggtct ccagctctgt     2580

aaccagtcct gtcccatttc ctcagtccct gggcctccca gccttcaggc tgtagggctg     2640

ccttactaaa attgaaaaat ccacctctta acatctcttt cactttggtt ttgctaacac     2700

tgctctctgc tgccctccca tcctccctgt atccattcat gccctatctt tcattctcca     2760

ctcctaatcc ctctcctttc tggcatcctg gcctctcgtg gtcctcagcc cctcaccccc     2820

agtactgcag atctcacagt ttgccttcca gaagccagcc tatctctagc ccatggtttt     2880

ggagttcctc tcgggttatc tcccacgcct gacctggaac cagcaagccc ctttcctgcc     2940

ttcttacccc caactctagg gatgggactg ttacaatact tcaagatcac tctttacacc     3000

tcttcaaagc aaagtcatga caatgcaggg ctcctcattg ctcccatctg cctctgctgc     3060

acacacaggc accagcaggg atgccacagg agtgcccaca gggtgcagga ctccactgat     3120

gagagatcca gccaaagagc tgcccccagg ggtatgaggg caccagctgg gttctccagg     3180

gagcaggagt tggacctcca tggagccact aggcctggcc tcctctacac atccccaggg     3240

ctatctggtt aattccatca agctcagagt taaaaggcat atcagcctgg agtatttggg     3300

agagactggc tgcagatccc cgccagccaa gatgcaagcc actcgggacc tgatgtcggc     3360

agctgtgcct ctactgccct gaggacttac cagagggagc cctactggcc ttcccccacc     3420

acagcagccc tgcctgtgaa gctcttgttt ctgacatttc acaggcagag aggtgccatc     3480

agttcgcctc cattccttgc caccatgacc agcctctccc tgaactctct cttgctcggg     3540

acctgcctga gggctccctg ctgcagttcg ccgtacttcc atctgctggg tgcctccatc     3600

gttggttggg tggggatggg gcattttctg agctaagctt tgtcattagt ttgtgaagca     3660

cctggtcagc aacctgcccc agacctggag ggtctttgtg gactgaaggt agacaccagc     3720

cagcatggtg gccctgttct gggggagcag ggtaaggcag gaggaagtgg gtgagctccg     3780

agatgatgag cacatgaagc ctgtggcccc ttcgtacctg caatatgtca ggagcctcac     3840

gctcacccaa gatcctgcag gggccaggct ccatctcact ggctctgagg gcaggacagg     3900

gtatcacaca tttctcacca ggcctccttt cctatgggca ttggtgcctc ccagaggttt     3960

cttgggctgc tggctggtga gagaggaccc ttaaagaaga tcaagccaag ctgaccttgg     4020

accctgtcca gcacagcttc tggcacagga tgcttggtga atgtaccctt tctttccctc     4080

cctgcagctc tgagggagcc cctgaccttg tagtgggtgg aggaggtaag gggcctccct     4140

ccctaaatct gcctcttctg caagctactt ggagacttgc ctagttgtac ccacccctcc     4200

aggtccctgg tgctagagct tctgagaagg gcctttccct ttcctctttg cctgctatat     4260

aaggcaggct cctgtggctc tgctggctca gtgtgggctg caggaggact gcagactcag     4320

ctgcaattct gaggggggtt tgggaggctt gtgcgaggtc tcaggcctgt gtggggagct     4380

ggtgcctctt cctgcccgta tctttctctt ccaagggcag tgctccaagg cagggactgg     4440

agaagccaag gggagagtct aaaagggcta gagcattttt aaaaatagac acagggtctt     4500

gggactgggg tttcggattg agttgcaagc agggagaaaa cctgaaggtc ggtgccccta     4560

tggggctgac cagtagagaa tttcctttac tgtatttttg tgtctggtct tccctttctg     4620

gcttctagga catccatgcc aggtgaggtg cctgggtccc tgttacaagt caggagccct     4680

gtagggagac ccctcctttt gtacaagtac ctgaatgctg cgacaagcag atttttgtaa     4740

aattttatat tagtttttaa tgtcagtggc gactcggttc ctggggctgc agccagcctg     4800

ggacttttgt aagaattttt gggtgactca cttagatgtc gtttccttct tgccccctct     4860

tcctctctgt aatctaagtg cattaaacat ctttgcag                             4898


<210> 45
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Gli3 siRNA #5"

<400> 45
gaacaacccu agucaagga                                                    19


<210> 46
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Gli3 siRNA #6"

<400> 46
gcucuaccgu gcagaauua                                                    19


<210> 47
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Gli3 siRNA #7"

<400> 47
gcuuagugcu gcaaaauua                                                    19


<210> 48
<211> 19
<212> RNA
<213> Mus musculus

<220>
<221> source
<223> /note="Mouse Gli3 siRNA #8"

<400> 48
ccucacauau caacaucua                                                    19


