               SEQUENCE LISTING

<110> Celltrend GmbH

<120> Diagnosis of cancer by detecting auto-antibodies against epidermal growth factor (EGF)

<130> U2886 PCT BLN

<150> EP14 15 3822.3
<151> 2014-02-04

<160> 2

<170> BiSSAP 1.3

<210> 1
<211> 1166
<212> PRT
<213> Homo sapiens

<220> 
<223> Amino acid sequence of the human EGF

<400> 1
Met Leu Leu Thr Leu Ile Ile Leu Leu Pro Val Val Ser Lys Phe Ser 
1               5                   10                  15      
Phe Val Ser Leu Ser Ala Pro Gln His Trp Ser Cys Pro Glu Gly Thr 
            20                  25                  30          
Leu Ala Gly Asn Gly Asn Ser Thr Cys Val Gly Pro Ala Pro Phe Leu 
        35                  40                  45              
Ile Phe Ser His Gly Asn Ser Ile Phe Arg Ile Asp Thr Glu Gly Thr 
    50                  55                  60                  
Asn Tyr Glu Gln Leu Val Val Asp Ala Gly Val Ser Val Ile Met Asp 
65                  70                  75                  80  
Phe His Tyr Asn Glu Lys Arg Ile Tyr Trp Val Asp Leu Glu Arg Gln 
                85                  90                  95      
Leu Leu Gln Arg Val Phe Leu Asn Gly Ser Arg Gln Glu Arg Val Cys 
            100                 105                 110         
Asn Ile Glu Lys Asn Val Ser Gly Met Ala Ile Asn Trp Ile Asn Glu 
        115                 120                 125             
Glu Val Ile Trp Ser Asn Gln Gln Glu Gly Ile Ile Thr Val Thr Asp 
    130                 135                 140                 
Met Lys Gly Asn Asn Ser His Ile Leu Leu Ser Ala Leu Lys Tyr Pro 
145                 150                 155                 160 
Ala Asn Val Ala Val Asp Pro Val Glu Arg Phe Ile Phe Trp Ser Ser 
                165                 170                 175     
Glu Val Ala Gly Ser Leu Tyr Arg Ala Asp Leu Asp Gly Val Gly Val 
            180                 185                 190         
Lys Ala Leu Leu Glu Thr Ser Glu Lys Ile Thr Ala Val Ser Leu Asp 
        195                 200                 205             
Val Leu Asp Lys Arg Leu Phe Trp Ile Gln Tyr Asn Arg Glu Gly Ser 
    210                 215                 220                 
Asn Ser Leu Ile Cys Ser Cys Asp Tyr Asp Gly Gly Ser Val His Ile 
225                 230                 235                 240 
Ser Lys His Pro Thr Gln His Asn Leu Phe Ala Met Ser Leu Phe Gly 
                245                 250                 255     
Asp Arg Ile Phe Tyr Ser Thr Trp Lys Met Lys Thr Ile Trp Ile Ala 
            260                 265                 270         
Asn Lys His Thr Gly Lys Asp Met Val Arg Ile Asn Leu His Ser Ser 
        275                 280                 285             
Phe Val Pro Leu Gly Glu Leu Lys Val Val His Pro Leu Ala Gln Pro 
    290                 295                 300                 
Lys Ala Glu Asp Asp Thr Trp Glu Pro Glu Gln Lys Leu Cys Lys Leu 
305                 310                 315                 320 
Arg Lys Gly Asn Cys Ser Ser Thr Val Cys Gly Gln Asp Leu Gln Ser 
                325                 330                 335     
His Leu Cys Met Cys Ala Glu Gly Tyr Ala Leu Ser Arg Asp Arg Lys 
            340                 345                 350         
Tyr Cys Glu Asp Val Asn Glu Cys Ala Phe Trp Asn His Gly Cys Thr 
        355                 360                 365             
Leu Gly Cys Lys Asn Thr Pro Gly Ser Tyr Tyr Cys Thr Cys Pro Val 
    370                 375                 380                 
Gly Phe Val Leu Leu Pro Asp Gly Lys Arg Cys His Gln Leu Val Ser 
385                 390                 395                 400 
Cys Pro Arg Asn Val Ser Glu Cys Ser His Asp Cys Val Leu Thr Ser 
                405                 410                 415     
Glu Gly Pro Leu Cys Phe Cys Pro Glu Gly Ser Val Leu Glu Arg Asp 
            420                 425                 430         
Gly Lys Thr Cys Ser Gly Cys Ser Ser Pro Asp Asn Gly Gly Cys Ser 
        435                 440                 445             
Gln Leu Cys Val Pro Leu Ser Pro Val Ser Trp Glu Cys Asp Cys Phe 
    450                 455                 460                 
Pro Gly Tyr Asp Leu Gln Leu Asp Glu Lys Ser Cys Ala Ala Ser Gly 
465                 470                 475                 480 
Pro Gln Pro Phe Leu Leu Phe Ala Asn Ser Gln Asp Ile Arg His Met 
                485                 490                 495     
His Phe Asp Gly Thr Asp Tyr Gly Thr Leu Leu Ser Gln Gln Met Gly 
            500                 505                 510         
Met Val Tyr Ala Leu Asp His Asp Pro Val Glu Asn Lys Ile Tyr Phe 
        515                 520                 525             
Ala His Thr Ala Leu Lys Trp Ile Glu Arg Ala Asn Met Asp Gly Ser 
    530                 535                 540                 
Gln Arg Glu Arg Leu Ile Glu Glu Gly Val Asp Val Pro Glu Gly Leu 
545                 550                 555                 560 
Ala Val Asp Trp Ile Gly Arg Arg Phe Tyr Trp Thr Asp Arg Gly Lys 
                565                 570                 575     
Ser Leu Ile Gly Arg Ser Asp Leu Asn Gly Lys Arg Ser Lys Ile Ile 
            580                 585                 590         
Thr Lys Glu Asn Ile Ser Gln Pro Arg Gly Ile Ala Val His Pro Met 
        595                 600                 605             
Ala Lys Arg Leu Phe Trp Thr Asp Thr Gly Ile Asn Pro Arg Ile Glu 
    610                 615                 620                 
Ser Ser Ser Leu Gln Gly Leu Gly Arg Leu Val Ile Ala Ser Ser Asp 
625                 630                 635                 640 
Leu Ile Trp Pro Ser Gly Ile Thr Ile Asp Phe Leu Thr Asp Lys Leu 
                645                 650                 655     
Tyr Trp Cys Asp Ala Lys Gln Ser Val Ile Glu Met Ala Asn Leu Asp 
            660                 665                 670         
Gly Ser Lys Arg Arg Arg Leu Thr Gln Asn Asp Val Gly His Pro Phe 
        675                 680                 685             
Ala Val Ala Val Phe Glu Asp Tyr Val Trp Phe Ser Asp Trp Ala Met 
    690                 695                 700                 
Pro Ser Val Met Arg Val Asn Lys Arg Thr Gly Lys Asp Arg Val Arg 
705                 710                 715                 720 
Leu Gln Gly Ser Met Leu Lys Pro Ser Ser Leu Val Val Val His Pro 
                725                 730                 735     
Leu Ala Lys Pro Gly Ala Asp Pro Cys Leu Tyr Gln Asn Gly Gly Cys 
            740                 745                 750         
Glu His Ile Cys Lys Lys Arg Leu Gly Thr Ala Trp Cys Ser Cys Arg 
        755                 760                 765             
Glu Gly Phe Met Lys Ala Ser Asp Gly Lys Thr Cys Leu Ala Leu Asp 
    770                 775                 780                 
Gly His Gln Leu Leu Ala Gly Gly Glu Val Asp Leu Lys Asn Gln Val 
785                 790                 795                 800 
Thr Pro Leu Asp Ile Leu Ser Lys Thr Arg Val Ser Glu Asp Asn Ile 
                805                 810                 815     
Thr Glu Ser Gln His Met Leu Val Ala Glu Ile Met Val Ser Asp Gln 
            820                 825                 830         
Asp Asp Cys Ala Pro Val Gly Cys Ser Met Tyr Ala Arg Cys Ile Ser 
        835                 840                 845             
Glu Gly Glu Asp Ala Thr Cys Gln Cys Leu Lys Gly Phe Ala Gly Asp 
    850                 855                 860                 
Gly Lys Leu Cys Ser Asp Ile Asp Glu Cys Glu Met Gly Val Pro Val 
865                 870                 875                 880 
Cys Pro Pro Ala Ser Ser Lys Cys Ile Asn Thr Glu Gly Gly Tyr Val 
                885                 890                 895     
Cys Arg Cys Ser Glu Gly Tyr Gln Gly Asp Gly Ile His Cys Leu Asp 
            900                 905                 910         
Ser Thr Pro Pro Pro His Leu Arg Glu Asp Asp His His Tyr Ser Val 
        915                 920                 925             
Arg Asn Ser Asp Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu 
    930                 935                 940                 
His Asp Gly Val Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys 
945                 950                 955                 960 
Asn Cys Val Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu 
                965                 970                 975     
Lys Trp Trp Glu Leu Arg His Ala Gly His Gly Gln Gln Gln Lys Val 
            980                 985                 990         
Ile Val Val Ala Val Cys Val Val Val Leu Val Met Leu Leu Leu Leu 
        995                 1000                1005            
Ser Leu Trp Gly Ala His Tyr Tyr Arg Thr Gln Lys Leu Leu Ser Lys 
    1010                1015                1020                
Asn Pro Lys Asn Pro Tyr Glu Glu Ser Ser Arg Asp Val Arg Ser Arg 
1025                1030                1035                1040
Arg Pro Ala Asp Thr Glu Asp Gly Met Ser Ser Cys Pro Gln Pro Trp 
                1045                1050                1055    
Phe Val Val Ile Lys Glu His Gln Asp Leu Lys Asn Gly Gly Gln Pro 
            1060                1065                1070        
Val Ala Gly Glu Asp Gly Gln Ala Ala Asp Gly Ser Met Gln Pro Thr 
        1075                1080                1085            
Ser Trp Arg Gln Glu Pro Gln Leu Cys Gly Met Gly Thr Glu Gln Gly 
    1090                1095                1100                
Cys Trp Ile Pro Val Ser Ser Asp Lys Gly Ser Cys Pro Gln Val Met 
1105                1110                1115                1120
Glu Arg Ser Phe His Met Pro Ser Tyr Gly Thr Gln Thr Leu Glu Gly 
                1125                1130                1135    
Gly Val Glu Lys Pro His Ser Leu Leu Ser Ala Asn Pro Leu Trp Gln 
            1140                1145                1150        
Gln Arg Ala Leu Asp Pro Pro His Gln Met Glu Leu Thr Gln 
        1155                1160                1165    

<210> 2
<211> 53
<212> PRT
<213> Homo sapiens

<220> 
<223> Amino acid sequence of the human mature EGF peptide [amino acids
      930 to 982 of SEQ ID NO:1]

<400> 2
Asn Ser Asp Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His 
1               5                   10                  15      
Asp Gly Val Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn 
            20                  25                  30          
Cys Val Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys 
        35                  40                  45              
Trp Trp Glu Leu Arg 
    50              

