                         SEQUENCE LISTING



<110>  PROTALIX LTD.

       SHAALTIEL, Yoseph

       ALMON, Einat

 

<120>  METHOD OF MAINTAINING DISEASE STABILITY IN A SUBJECT HAVING 

       GAUCHER'S DISEASE



<130>  61637



<150>  US 61/937,901

<151>  2014-02-10



<160>  15    



<170>  PatentIn version 3.5



<210>  1

<211>  22

<212>  PRT

<213>  Artificial sequence



<220>

<223>  ER signal peptide



<400>  1



Met Lys Thr Asn Leu Phe Leu Phe Leu Ile Phe Ser Leu Leu Leu Ser 

1               5                   10                  15      





Leu Ser Ser Ala Glu Phe 

            20          





<210>  2

<211>  7

<212>  PRT

<213>  Artificial sequence



<220>

<223>  Vacuolar targeting signal from Tobacco chitinase A



<400>  2



Asp Leu Leu Val Asp Thr Met 

1               5           





<210>  3

<211>  1491

<212>  DNA

<213>  Homo sapiens



<400>  3

gcccgcccct gcatccctaa aagcttcggc tacagctcgg tggtgtgtgt ctgcaatgcc       60



acatactgtg actcctttga ccccccgacc tttcctgccc ttggtacctt cagccgctat      120



gagagtacac gcagtgggcg acggatggag ctgagtatgg ggcccatcca ggctaatcac      180



acgggcacag gcctgctact gaccctgcag ccagaacaga agttccagaa agtgaaggga      240



tttggagggg ccatgacaga tgctgctgct ctcaacatcc ttgccctgtc accccctgcc      300



caaaatttgc tacttaaatc gtacttctct gaagaaggaa tcggatataa catcatccgg      360



gtacccatgg ccagctgtga cttctccatc cgcacctaca cctatgcaga cacccctgat      420



gatttccagt tgcacaactt cagcctccca gaggaagata ccaagctcaa gatacccctg      480



attcaccgag ccctgcagtt ggcccagcgt cccgtttcac tccttgccag cccctggaca      540



tcacccactt ggctcaagac caatggagcg gtgaatggga aggggtcact caagggacag      600



cccggagaca tctaccacca gacctgggcc agatactttg tgaagttcct ggatgcctat      660



gctgagcaca agttacagtt ctgggcagtg acagctgaaa atgagccttc tgctgggctg      720



ttgagtggat accccttcca gtgcctgggc ttcacccctg aacatcagcg agacttcatt      780



gcccgtgacc taggtcctac cctcgccaac agtactcacc acaatgtccg cctactcatg      840



ctggatgacc aacgcttgct gctgccccac tgggcaaagg tggtactgac agacccagaa      900



gcagctaaat atgttcatgg cattgctgta cattggtacc tggactttct ggctccagcc      960



aaagccaccc taggggagac acaccgcctg ttccccaaca ccatgctctt tgcctcagag     1020



gcctgtgtgg gctccaagtt ctgggagcag agtgtgcggc taggctcctg ggatcgaggg     1080



atgcagtaca gccacagcat catcacgaac ctcctgtacc atgtggtcgg ctggaccgac     1140



tggaaccttg ccctgaaccc cgaaggagga cccaattggg tgcgtaactt tgtcgacagt     1200



cccatcattg tagacatcac caaggacacg ttttacaaac agcccatgtt ctaccacctt     1260



ggccacttca gcaagttcat tcctgagggc tcccagagag tggggctggt tgccagtcag     1320



aagaacgacc tggacgcagt ggcactgatg catcccgatg gctctgctgt tgtggtcgtg     1380



ctaaaccgct cctctaagga tgtgcctctt accatcaagg atcctgctgt gggcttcctg     1440



gagacaatct cacctggcta ctccattcac acctacctgt ggcatcgcca g              1491





<210>  4

<211>  497

<212>  PRT

<213>  Artificial sequence



<220>

<223>  Human GCD H to R substitution at c' terminus



<400>  4



Ala Arg Pro Cys Ile Pro Lys Ser Phe Gly Tyr Ser Ser Val Val Cys 

1               5                   10                  15      





Val Cys Asn Ala Thr Tyr Cys Asp Ser Phe Asp Pro Pro Thr Phe Pro 

            20                  25                  30          





Ala Leu Gly Thr Phe Ser Arg Tyr Glu Ser Thr Arg Ser Gly Arg Arg 

        35                  40                  45              





Met Glu Leu Ser Met Gly Pro Ile Gln Ala Asn His Thr Gly Thr Gly 

    50                  55                  60                  





Leu Leu Leu Thr Leu Gln Pro Glu Gln Lys Phe Gln Lys Val Lys Gly 

65                  70                  75                  80  





Phe Gly Gly Ala Met Thr Asp Ala Ala Ala Leu Asn Ile Leu Ala Leu 

                85                  90                  95      





Ser Pro Pro Ala Gln Asn Leu Leu Leu Lys Ser Tyr Phe Ser Glu Glu 

            100                 105                 110         





Gly Ile Gly Tyr Asn Ile Ile Arg Val Pro Met Ala Ser Cys Asp Phe 

        115                 120                 125             





Ser Ile Arg Thr Tyr Thr Tyr Ala Asp Thr Pro Asp Asp Phe Gln Leu 

    130                 135                 140                 





His Asn Phe Ser Leu Pro Glu Glu Asp Thr Lys Leu Lys Ile Pro Leu 

145                 150                 155                 160 





Ile His Arg Ala Leu Gln Leu Ala Gln Arg Pro Val Ser Leu Leu Ala 

                165                 170                 175     





Ser Pro Trp Thr Ser Pro Thr Trp Leu Lys Thr Asn Gly Ala Val Asn 

            180                 185                 190         





Gly Lys Gly Ser Leu Lys Gly Gln Pro Gly Asp Ile Tyr His Gln Thr 

        195                 200                 205             





Trp Ala Arg Tyr Phe Val Lys Phe Leu Asp Ala Tyr Ala Glu His Lys 

    210                 215                 220                 





Leu Gln Phe Trp Ala Val Thr Ala Glu Asn Glu Pro Ser Ala Gly Leu 

225                 230                 235                 240 





Leu Ser Gly Tyr Pro Phe Gln Cys Leu Gly Phe Thr Pro Glu His Gln 

                245                 250                 255     





Arg Asp Phe Ile Ala Arg Asp Leu Gly Pro Thr Leu Ala Asn Ser Thr 

            260                 265                 270         





His His Asn Val Arg Leu Leu Met Leu Asp Asp Gln Arg Leu Leu Leu 

        275                 280                 285             





Pro His Trp Ala Lys Val Val Leu Thr Asp Pro Glu Ala Ala Lys Tyr 

    290                 295                 300                 





Val His Gly Ile Ala Val His Trp Tyr Leu Asp Phe Leu Ala Pro Ala 

305                 310                 315                 320 





Lys Ala Thr Leu Gly Glu Thr His Arg Leu Phe Pro Asn Thr Met Leu 

                325                 330                 335     





Phe Ala Ser Glu Ala Cys Val Gly Ser Lys Phe Trp Glu Gln Ser Val 

            340                 345                 350         





Arg Leu Gly Ser Trp Asp Arg Gly Met Gln Tyr Ser His Ser Ile Ile 

        355                 360                 365             





Thr Asn Leu Leu Tyr His Val Val Gly Trp Thr Asp Trp Asn Leu Ala 

    370                 375                 380                 





Leu Asn Pro Glu Gly Gly Pro Asn Trp Val Arg Asn Phe Val Asp Ser 

385                 390                 395                 400 





Pro Ile Ile Val Asp Ile Thr Lys Asp Thr Phe Tyr Lys Gln Pro Met 

                405                 410                 415     





Phe Tyr His Leu Gly His Phe Ser Lys Phe Ile Pro Glu Gly Ser Gln 

            420                 425                 430         





Arg Val Gly Leu Val Ala Ser Gln Lys Asn Asp Leu Asp Ala Val Ala 

        435                 440                 445             





Leu Met His Pro Asp Gly Ser Ala Val Val Val Val Leu Asn Arg Ser 

    450                 455                 460                 





Ser Lys Asp Val Pro Leu Thr Ile Lys Asp Pro Ala Val Gly Phe Leu 

465                 470                 475                 480 





Glu Thr Ile Ser Pro Gly Tyr Ser Ile His Thr Tyr Leu Trp His Arg 

                485                 490                 495     





Gln 

    





<210>  5

<211>  338

<212>  DNA

<213>  Cauliflower mosaic virus



<400>  5

ttttcacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac       60



ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa      120



ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc      180



acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga      240



tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct      300



tcctctatat aaggaagttc atttcatttg gagaggac                              338





<210>  6

<211>  66

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Nucleic acid sequence encoding the ER signal peptide



<400>  6

atgaagacta atctttttct ctttctcatc ttttcacttc tcctatcatt atcctcggcc       60



gaattc                                                                  66





<210>  7

<211>  21

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Nucleic acid sequence encoding the vacuolar targeting sequence



<400>  7

gatcttttag tcgatactat g                                                 21





<210>  8

<211>  167

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Nucleic acid sequence of the Agrobacterium tumefaciens terminator





<220>

<221>  misc_feature

<222>  (162)..(162)

<223>  n is a, c, g, or t



<400>  8

taatttcatg atctgttttg ttgtattccc ttgcaatgca gggcctaggg ctatgaataa       60



agttaatgtg tgaatgtgtg aatgtgtgat tgtgacctga agggatcacg actataatcg      120



tttataataa acaaagactt tgtcccaaaa accccccccc cngcaga                    167





<210>  9

<211>  2186

<212>  DNA

<213>  Artificial sequence



<220>

<223>  nucleic acid sequence encoding high mannose human 

       glucocerebrosidase (GCD)





<220>

<221>  misc_feature

<222>  (2181)..(2181)

<223>  n is a, c, g, or t



<400>  9

ttttcacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac       60



ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa      120



ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc      180



acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga      240



tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct      300



tcctctatat aaggaagttc atttcatttg gagaggacag gcttcttgag atccttcaac      360



aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa ttacagtcga      420



gggatccaag gagatataac aatgaagact aatctttttc tctttctcat cttttcactt      480



ctcctatcat tatcctcggc cgaattcgcc cgcccctgca tccctaaaag cttcggctac      540



agctcggtgg tgtgtgtctg caatgccaca tactgtgact cctttgaccc cccgaccttt      600



cctgcccttg gtaccttcag ccgctatgag agtacacgca gtgggcgacg gatggagctg      660



agtatggggc ccatccaggc taatcacacg ggcacaggcc tgctactgac cctgcagcca      720



gaacagaagt tccagaaagt gaagggattt ggaggggcca tgacagatgc tgctgctctc      780



aacatccttg ccctgtcacc ccctgcccaa aatttgctac ttaaatcgta cttctctgaa      840



gaaggaatcg gatataacat catccgggta cccatggcca gctgtgactt ctccatccgc      900



acctacacct atgcagacac ccctgatgat ttccagttgc acaacttcag cctcccagag      960



gaagatacca agctcaagat acccctgatt caccgagccc tgcagttggc ccagcgtccc     1020



gtttcactcc ttgccagccc ctggacatca cccacttggc tcaagaccaa tggagcggtg     1080



aatgggaagg ggtcactcaa gggacagccc ggagacatct accaccagac ctgggccaga     1140



tactttgtga agttcctgga tgcctatgct gagcacaagt tacagttctg ggcagtgaca     1200



gctgaaaatg agccttctgc tgggctgttg agtggatacc ccttccagtg cctgggcttc     1260



acccctgaac atcagcgaga cttcattgcc cgtgacctag gtcctaccct cgccaacagt     1320



actcaccaca atgtccgcct actcatgctg gatgaccaac gcttgctgct gccccactgg     1380



gcaaaggtgg tactgacaga cccagaagca gctaaatatg ttcatggcat tgctgtacat     1440



tggtacctgg actttctggc tccagccaaa gccaccctag gggagacaca ccgcctgttc     1500



cccaacacca tgctctttgc ctcagaggcc tgtgtgggct ccaagttctg ggagcagagt     1560



gtgcggctag gctcctggga tcgagggatg cagtacagcc acagcatcat cacgaacctc     1620



ctgtaccatg tggtcggctg gaccgactgg aaccttgccc tgaaccccga aggaggaccc     1680



aattgggtgc gtaactttgt cgacagtccc atcattgtag acatcaccaa ggacacgttt     1740



tacaaacagc ccatgttcta ccaccttggc cacttcagca agttcattcc tgagggctcc     1800



cagagagtgg ggctggttgc cagtcagaag aacgacctgg acgcagtggc actgatgcat     1860



cccgatggct ctgctgttgt ggtcgtgcta aaccgctcct ctaaggatgt gcctcttacc     1920



atcaaggatc ctgctgtggg cttcctggag acaatctcac ctggctactc cattcacacc     1980



tacctgtggc atcgccaaga tcttttagtc gatactatgt aatttcatga tctgttttgt     2040



tgtattccct tgcaatgcag ggcctagggc tatgaataaa gttaatgtgt gaatgtgtga     2100



atgtgtgatt gtgacctgaa gggatcacga ctataatcgt ttataataaa caaagacttt     2160



gtcccaaaaa cccccccccc ngcaga                                          2186





<210>  10

<211>  526

<212>  PRT

<213>  Artificial sequence



<220>

<223>  High mannose human glucocerebrosidase (GCD)



<400>  10



Met Lys Thr Asn Leu Phe Leu Phe Leu Ile Phe Ser Leu Leu Leu Ser 

1               5                   10                  15      





Leu Ser Ser Ala Glu Phe Ala Arg Pro Cys Ile Pro Lys Ser Phe Gly 

            20                  25                  30          





Tyr Ser Ser Val Val Cys Val Cys Asn Ala Thr Tyr Cys Asp Ser Phe 

        35                  40                  45              





Asp Pro Pro Thr Phe Pro Ala Leu Gly Thr Phe Ser Arg Tyr Glu Ser 

    50                  55                  60                  





Thr Arg Ser Gly Arg Arg Met Glu Leu Ser Met Gly Pro Ile Gln Ala 

65                  70                  75                  80  





Asn His Thr Gly Thr Gly Leu Leu Leu Thr Leu Gln Pro Glu Gln Lys 

                85                  90                  95      





Phe Gln Lys Val Lys Gly Phe Gly Gly Ala Met Thr Asp Ala Ala Ala 

            100                 105                 110         





Leu Asn Ile Leu Ala Leu Ser Pro Pro Ala Gln Asn Leu Leu Leu Lys 

        115                 120                 125             





Ser Tyr Phe Ser Glu Glu Gly Ile Gly Tyr Asn Ile Ile Arg Val Pro 

    130                 135                 140                 





Met Ala Ser Cys Asp Phe Ser Ile Arg Thr Tyr Thr Tyr Ala Asp Thr 

145                 150                 155                 160 





Pro Asp Asp Phe Gln Leu His Asn Phe Ser Leu Pro Glu Glu Asp Thr 

                165                 170                 175     





Lys Leu Lys Ile Pro Leu Ile His Arg Ala Leu Gln Leu Ala Gln Arg 

            180                 185                 190         





Pro Val Ser Leu Leu Ala Ser Pro Trp Thr Ser Pro Thr Trp Leu Lys 

        195                 200                 205             





Thr Asn Gly Ala Val Asn Gly Lys Gly Ser Leu Lys Gly Gln Pro Gly 

    210                 215                 220                 





Asp Ile Tyr His Gln Thr Trp Ala Arg Tyr Phe Val Lys Phe Leu Asp 

225                 230                 235                 240 





Ala Tyr Ala Glu His Lys Leu Gln Phe Trp Ala Val Thr Ala Glu Asn 

                245                 250                 255     





Glu Pro Ser Ala Gly Leu Leu Ser Gly Tyr Pro Phe Gln Cys Leu Gly 

            260                 265                 270         





Phe Thr Pro Glu His Gln Arg Asp Phe Ile Ala Arg Asp Leu Gly Pro 

        275                 280                 285             





Thr Leu Ala Asn Ser Thr His His Asn Val Arg Leu Leu Met Leu Asp 

    290                 295                 300                 





Asp Gln Arg Leu Leu Leu Pro His Trp Ala Lys Val Val Leu Thr Asp 

305                 310                 315                 320 





Pro Glu Ala Ala Lys Tyr Val His Gly Ile Ala Val His Trp Tyr Leu 

                325                 330                 335     





Asp Phe Leu Ala Pro Ala Lys Ala Thr Leu Gly Glu Thr His Arg Leu 

            340                 345                 350         





Phe Pro Asn Thr Met Leu Phe Ala Ser Glu Ala Cys Val Gly Ser Lys 

        355                 360                 365             





Phe Trp Glu Gln Ser Val Arg Leu Gly Ser Trp Asp Arg Gly Met Gln 

    370                 375                 380                 





Tyr Ser His Ser Ile Ile Thr Asn Leu Leu Tyr His Val Val Gly Trp 

385                 390                 395                 400 





Thr Asp Trp Asn Leu Ala Leu Asn Pro Glu Gly Gly Pro Asn Trp Val 

                405                 410                 415     





Arg Asn Phe Val Asp Ser Pro Ile Ile Val Asp Ile Thr Lys Asp Thr 

            420                 425                 430         





Phe Tyr Lys Gln Pro Met Phe Tyr His Leu Gly His Phe Ser Lys Phe 

        435                 440                 445             





Ile Pro Glu Gly Ser Gln Arg Val Gly Leu Val Ala Ser Gln Lys Asn 

    450                 455                 460                 





Asp Leu Asp Ala Val Ala Leu Met His Pro Asp Gly Ser Ala Val Val 

465                 470                 475                 480 





Val Val Leu Asn Arg Ser Ser Lys Asp Val Pro Leu Thr Ile Lys Asp 

                485                 490                 495     





Pro Ala Val Gly Phe Leu Glu Thr Ile Ser Pro Gly Tyr Ser Ile His 

            500                 505                 510         





Thr Tyr Leu Trp His Arg Gln Asp Leu Leu Val Asp Thr Met 

        515                 520                 525     





<210>  11

<211>  506

<212>  PRT

<213>  Artificial sequence



<220>

<223>  Processed plant produced human recombinant GCD protein



<400>  11



Glu Phe Ala Arg Pro Cys Ile Pro Lys Ser Phe Gly Tyr Ser Ser Val 

1               5                   10                  15      





Val Cys Val Cys Asn Ala Thr Tyr Cys Asp Ser Phe Asp Pro Pro Thr 

            20                  25                  30          





Phe Pro Ala Leu Gly Thr Phe Ser Arg Tyr Glu Ser Thr Arg Ser Gly 

        35                  40                  45              





Arg Arg Met Glu Leu Ser Met Gly Pro Ile Gln Ala Asn His Thr Gly 

    50                  55                  60                  





Thr Gly Leu Leu Leu Thr Leu Gln Pro Glu Gln Lys Phe Gln Lys Val 

65                  70                  75                  80  





Lys Gly Phe Gly Gly Ala Met Thr Asp Ala Ala Ala Leu Asn Ile Leu 

                85                  90                  95      





Ala Leu Ser Pro Pro Ala Gln Asn Leu Leu Leu Lys Ser Tyr Phe Ser 

            100                 105                 110         





Glu Glu Gly Ile Gly Tyr Asn Ile Ile Arg Val Pro Met Ala Ser Cys 

        115                 120                 125             





Asp Phe Ser Ile Arg Thr Tyr Thr Tyr Ala Asp Thr Pro Asp Asp Phe 

    130                 135                 140                 





Gln Leu His Asn Phe Ser Leu Pro Glu Glu Asp Thr Lys Leu Lys Ile 

145                 150                 155                 160 





Pro Leu Ile His Arg Ala Leu Gln Leu Ala Gln Arg Pro Val Ser Leu 

                165                 170                 175     





Leu Ala Ser Pro Trp Thr Ser Pro Thr Trp Leu Lys Thr Asn Gly Ala 

            180                 185                 190         





Val Asn Gly Lys Gly Ser Leu Lys Gly Gln Pro Gly Asp Ile Tyr His 

        195                 200                 205             





Gln Thr Trp Ala Arg Tyr Phe Val Lys Phe Leu Asp Ala Tyr Ala Glu 

    210                 215                 220                 





His Lys Leu Gln Phe Trp Ala Val Thr Ala Glu Asn Glu Pro Ser Ala 

225                 230                 235                 240 





Gly Leu Leu Ser Gly Tyr Pro Phe Gln Cys Leu Gly Phe Thr Pro Glu 

                245                 250                 255     





His Gln Arg Asp Phe Ile Ala Arg Asp Leu Gly Pro Thr Leu Ala Asn 

            260                 265                 270         





Ser Thr His His Asn Val Arg Leu Leu Met Leu Asp Asp Gln Arg Leu 

        275                 280                 285             





Leu Leu Pro His Trp Ala Lys Val Val Leu Thr Asp Pro Glu Ala Ala 

    290                 295                 300                 





Lys Tyr Val His Gly Ile Ala Val His Trp Tyr Leu Asp Phe Leu Ala 

305                 310                 315                 320 





Pro Ala Lys Ala Thr Leu Gly Glu Thr His Arg Leu Phe Pro Asn Thr 

                325                 330                 335     





Met Leu Phe Ala Ser Glu Ala Cys Val Gly Ser Lys Phe Trp Glu Gln 

            340                 345                 350         





Ser Val Arg Leu Gly Ser Trp Asp Arg Gly Met Gln Tyr Ser His Ser 

        355                 360                 365             





Ile Ile Thr Asn Leu Leu Tyr His Val Val Gly Trp Thr Asp Trp Asn 

    370                 375                 380                 





Leu Ala Leu Asn Pro Glu Gly Gly Pro Asn Trp Val Arg Asn Phe Val 

385                 390                 395                 400 





Asp Ser Pro Ile Ile Val Asp Ile Thr Lys Asp Thr Phe Tyr Lys Gln 

                405                 410                 415     





Pro Met Phe Tyr His Leu Gly His Phe Ser Lys Phe Ile Pro Glu Gly 

            420                 425                 430         





Ser Gln Arg Val Gly Leu Val Ala Ser Gln Lys Asn Asp Leu Asp Ala 

        435                 440                 445             





Val Ala Leu Met His Pro Asp Gly Ser Ala Val Val Val Val Leu Asn 

    450                 455                 460                 





Arg Ser Ser Lys Asp Val Pro Leu Thr Ile Lys Asp Pro Ala Val Gly 

465                 470                 475                 480 





Phe Leu Glu Thr Ile Ser Pro Gly Tyr Ser Ile His Thr Tyr Leu Trp 

                485                 490                 495     





His Arg Gln Asp Leu Leu Val Asp Thr Met 

            500                 505     





<210>  12

<211>  20

<212>  PRT

<213>  Artificial sequence



<220>

<223>  ER signal peptide



<400>  12



Met Lys Thr Asn Leu Phe Leu Phe Leu Ile Phe Ser Leu Leu Leu Ser 

1               5                   10                  15      





Leu Ser Ser Ala 

            20  





<210>  13

<211>  497

<212>  PRT

<213>  Homo sapiens



<400>  13



Ala Arg Pro Cys Ile Pro Lys Ser Phe Gly Tyr Ser Ser Val Val Cys 

1               5                   10                  15      





Val Cys Asn Ala Thr Tyr Cys Asp Ser Phe Asp Pro Pro Thr Phe Pro 

            20                  25                  30          





Ala Leu Gly Thr Phe Ser Arg Tyr Glu Ser Thr Arg Ser Gly Arg Arg 

        35                  40                  45              





Met Glu Leu Ser Met Gly Pro Ile Gln Ala Asn His Thr Gly Thr Gly 

    50                  55                  60                  





Leu Leu Leu Thr Leu Gln Pro Glu Gln Lys Phe Gln Lys Val Lys Gly 

65                  70                  75                  80  





Phe Gly Gly Ala Met Thr Asp Ala Ala Ala Leu Asn Ile Leu Ala Leu 

                85                  90                  95      





Ser Pro Pro Ala Gln Asn Leu Leu Leu Lys Ser Tyr Phe Ser Glu Glu 

            100                 105                 110         





Gly Ile Gly Tyr Asn Ile Ile Arg Val Pro Met Ala Ser Cys Asp Phe 

        115                 120                 125             





Ser Ile Arg Thr Tyr Thr Tyr Ala Asp Thr Pro Asp Asp Phe Gln Leu 

    130                 135                 140                 





His Asn Phe Ser Leu Pro Glu Glu Asp Thr Lys Leu Lys Ile Pro Leu 

145                 150                 155                 160 





Ile His Arg Ala Leu Gln Leu Ala Gln Arg Pro Val Ser Leu Leu Ala 

                165                 170                 175     





Ser Pro Trp Thr Ser Pro Thr Trp Leu Lys Thr Asn Gly Ala Val Asn 

            180                 185                 190         





Gly Lys Gly Ser Leu Lys Gly Gln Pro Gly Asp Ile Tyr His Gln Thr 

        195                 200                 205             





Trp Ala Arg Tyr Phe Val Lys Phe Leu Asp Ala Tyr Ala Glu His Lys 

    210                 215                 220                 





Leu Gln Phe Trp Ala Val Thr Ala Glu Asn Glu Pro Ser Ala Gly Leu 

225                 230                 235                 240 





Leu Ser Gly Tyr Pro Phe Gln Cys Leu Gly Phe Thr Pro Glu His Gln 

                245                 250                 255     





Arg Asp Phe Ile Ala Arg Asp Leu Gly Pro Thr Leu Ala Asn Ser Thr 

            260                 265                 270         





His His Asn Val Arg Leu Leu Met Leu Asp Asp Gln Arg Leu Leu Leu 

        275                 280                 285             





Pro His Trp Ala Lys Val Val Leu Thr Asp Pro Glu Ala Ala Lys Tyr 

    290                 295                 300                 





Val His Gly Ile Ala Val His Trp Tyr Leu Asp Phe Leu Ala Pro Ala 

305                 310                 315                 320 





Lys Ala Thr Leu Gly Glu Thr His Arg Leu Phe Pro Asn Thr Met Leu 

                325                 330                 335     





Phe Ala Ser Glu Ala Cys Val Gly Ser Lys Phe Trp Glu Gln Ser Val 

            340                 345                 350         





Arg Leu Gly Ser Trp Asp Arg Gly Met Gln Tyr Ser His Ser Ile Ile 

        355                 360                 365             





Thr Asn Leu Leu Tyr His Val Val Gly Trp Thr Asp Trp Asn Leu Ala 

    370                 375                 380                 





Leu Asn Pro Glu Gly Gly Pro Asn Trp Val Arg Asn Phe Val Asp Ser 

385                 390                 395                 400 





Pro Ile Ile Val Asp Ile Thr Lys Asp Thr Phe Tyr Lys Gln Pro Met 

                405                 410                 415     





Phe Tyr His Leu Gly His Phe Ser Lys Phe Ile Pro Glu Gly Ser Gln 

            420                 425                 430         





Arg Val Gly Leu Val Ala Ser Gln Lys Asn Asp Leu Asp Ala Val Ala 

        435                 440                 445             





Leu Met His Pro Asp Gly Ser Ala Val Val Val Val Leu Asn Arg Ser 

    450                 455                 460                 





Ser Lys Asp Val Pro Leu Thr Ile Lys Asp Pro Ala Val Gly Phe Leu 

465                 470                 475                 480 





Glu Thr Ile Ser Pro Gly Tyr Ser Ile His Thr Tyr Leu Trp Arg Arg 

                485                 490                 495     





Gln 

    





<210>  14

<211>  536

<212>  PRT

<213>  Artificial sequence



<220>

<223>  Human GCD fused to an artificial leader sequence



<400>  14



Met Glu Phe Ser Ser Pro Ser Arg Glu Glu Cys Pro Lys Pro Leu Ser 

1               5                   10                  15      





Arg Val Ser Ile Met Ala Gly Ser Leu Thr Gly Leu Leu Leu Leu Gln 

            20                  25                  30          





Ala Val Ser Trp Ala Ser Gly Ala Arg Pro Cys Ile Pro Lys Ser Phe 

        35                  40                  45              





Gly Tyr Ser Ser Val Val Cys Val Cys Asn Ala Thr Tyr Cys Asp Ser 

    50                  55                  60                  





Phe Asp Pro Pro Thr Phe Pro Ala Leu Gly Thr Phe Ser Arg Tyr Glu 

65                  70                  75                  80  





Ser Thr Arg Ser Gly Arg Arg Met Glu Leu Ser Met Gly Pro Ile Gln 

                85                  90                  95      





Ala Asn His Thr Gly Thr Gly Leu Leu Leu Thr Leu Gln Pro Glu Gln 

            100                 105                 110         





Lys Phe Gln Lys Val Lys Gly Phe Gly Gly Ala Met Thr Asp Ala Ala 

        115                 120                 125             





Ala Leu Asn Ile Leu Ala Leu Ser Pro Pro Ala Gln Asn Leu Leu Leu 

    130                 135                 140                 





Lys Ser Tyr Phe Ser Glu Glu Gly Ile Gly Tyr Asn Ile Ile Arg Val 

145                 150                 155                 160 





Pro Met Ala Ser Cys Asp Phe Ser Ile Arg Thr Tyr Thr Tyr Ala Asp 

                165                 170                 175     





Thr Pro Asp Asp Phe Gln Leu His Asn Phe Ser Leu Pro Glu Glu Asp 

            180                 185                 190         





Thr Lys Leu Lys Ile Pro Leu Ile His Arg Ala Leu Gln Leu Ala Gln 

        195                 200                 205             





Arg Pro Val Ser Leu Leu Ala Ser Pro Trp Thr Ser Pro Thr Trp Leu 

    210                 215                 220                 





Lys Thr Asn Gly Ala Val Asn Gly Lys Gly Ser Leu Lys Gly Gln Pro 

225                 230                 235                 240 





Gly Asp Ile Tyr His Gln Thr Trp Ala Arg Tyr Phe Val Lys Phe Leu 

                245                 250                 255     





Asp Ala Tyr Ala Glu His Lys Leu Gln Phe Trp Ala Val Thr Ala Glu 

            260                 265                 270         





Asn Glu Pro Ser Ala Gly Leu Leu Ser Gly Tyr Pro Phe Gln Cys Leu 

        275                 280                 285             





Gly Phe Thr Pro Glu His Gln Arg Asp Phe Ile Ala Arg Asp Leu Gly 

    290                 295                 300                 





Pro Thr Leu Ala Asn Ser Thr His His Asn Val Arg Leu Leu Met Leu 

305                 310                 315                 320 





Asp Asp Gln Arg Leu Leu Leu Pro His Trp Ala Lys Val Val Leu Thr 

                325                 330                 335     





Asp Pro Glu Ala Ala Lys Tyr Val His Gly Ile Ala Val His Trp Tyr 

            340                 345                 350         





Leu Asp Phe Leu Ala Pro Ala Lys Ala Thr Leu Gly Glu Thr His Arg 

        355                 360                 365             





Leu Phe Pro Asn Thr Met Leu Phe Ala Ser Glu Ala Cys Val Gly Ser 

    370                 375                 380                 





Lys Phe Trp Glu Gln Ser Val Arg Leu Gly Ser Trp Asp Arg Gly Met 

385                 390                 395                 400 





Gln Tyr Ser His Ser Ile Ile Thr Asn Leu Leu Tyr His Val Val Gly 

                405                 410                 415     





Trp Thr Asp Trp Asn Leu Ala Leu Asn Pro Glu Gly Gly Pro Asn Trp 

            420                 425                 430         





Val Arg Asn Phe Val Asp Ser Pro Ile Ile Val Asp Ile Thr Lys Asp 

        435                 440                 445             





Thr Phe Tyr Lys Gln Pro Met Phe Tyr His Leu Gly His Phe Ser Lys 

    450                 455                 460                 





Phe Ile Pro Glu Gly Ser Gln Arg Val Gly Leu Val Ala Ser Gln Lys 

465                 470                 475                 480 





Asn Asp Leu Asp Ala Val Ala Leu Met His Pro Asp Gly Ser Ala Val 

                485                 490                 495     





Val Val Val Leu Asn Arg Ser Ser Lys Asp Val Pro Leu Thr Ile Lys 

            500                 505                 510         





Asp Pro Ala Val Gly Phe Leu Glu Thr Ile Ser Pro Gly Tyr Ser Ile 

        515                 520                 525             





His Thr Tyr Leu Trp Arg Arg Gln 

    530                 535     





<210>  15

<211>  1491

<212>  DNA

<213>  Homo sapiens



<400>  15

gcccgcccct gcatccctaa aagcttcggc tacagctcgg tggtgtgtgt ctgcaatgcc       60



acatactgtg actcctttga ccccccgacc tttcctgccc ttggtacctt cagccgctat      120



gagagtacac gcagtgggcg acggatggag ctgagtatgg ggcccatcca ggctaatcac      180



acgggcacag gcctgctact gaccctgcag ccagaacaga agttccagaa agtgaaggga      240



tttggagggg ccatgacaga tgctgctgct ctcaacatcc ttgccctgtc accccctgcc      300



caaaatttgc tacttaaatc gtacttctct gaagaaggaa tcggatataa catcatccgg      360



gtacccatgg ccagctgtga cttctccatc cgcacctaca cctatgcaga cacccctgat      420



gatttccagt tgcacaactt cagcctccca gaggaagata ccaagctcaa gatacccctg      480



attcaccgag ccctgcagtt ggcccagcgt cccgtttcac tccttgccag cccctggaca      540



tcacccactt ggctcaagac caatggagcg gtgaatggga aggggtcact caagggacag      600



cccggagaca tctaccacca gacctgggcc agatactttg tgaagttcct ggatgcctat      660



gctgagcaca agttacagtt ctgggcagtg acagctgaaa atgagccttc tgctgggctg      720



ttgagtggat accccttcca gtgcctgggc ttcacccctg aacatcagcg agacttcatt      780



gcccgtgacc taggtcctac cctcgccaac agtactcacc acaatgtccg cctactcatg      840



ctggatgacc aacgcttgct gctgccccac tgggcaaagg tggtactgac agacccagaa      900



gcagctaaat atgttcatgg cattgctgta cattggtacc tggactttct ggctccagcc      960



aaagccaccc taggggagac acaccgcctg ttccccaaca ccatgctctt tgcctcagag     1020



gcctgtgtgg gctccaagtt ctgggagcag agtgtgcggc taggctcctg ggatcgaggg     1080



atgcagtaca gccacagcat catcacgaac ctcctgtacc atgtggtcgg ctggaccgac     1140



tggaaccttg ccctgaaccc cgaaggagga cccaattggg tgcgtaactt tgtcgacagt     1200



cccatcattg tagacatcac caaggacacg ttttacaaac agcccatgtt ctaccacctt     1260



ggccacttca gcaagttcat tcctgagggc tcccagagag tggggctggt tgccagtcag     1320



aagaacgacc tggacgcagt ggcactgatg catcccgatg gctctgctgt tgtggtcgtg     1380



ctaaaccgct cctctaagga tgtgcctctt accatcaagg atcctgctgt gggcttcctg     1440



gagacaatct cacctggcta ctccattcac acctacctgt ggcgtcgcca g              1491





