                         SEQUENCE LISTING

<110>  Cellectis
 
<120>  METHOD FOR IN SITU INHIBITION OF REGULATORY T CELLS 

<130>  P81400503PCT00

<150>  PA201470088
<151>  2014-02-21

<160>  17     

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide inhibitor of FoxP3

<400>  1

Arg Asp Phe Gln Ser Phe Arg Lys Met Trp Pro Phe Phe Ala Met 
1               5                   10                  15  


<210>  2
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  peptide inhibitor of FoxP3

<400>  2
cgcgactttc aaagtttccg taagatgtgg ccgttttttg caatg                       45


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide inhibitor of FoxP3

<400>  3

Met Arg Asp Phe Gln Ser Phe Arg Lys Met Trp Pro Phe Phe Ala Met 
1               5                   10                  15      


<210>  4
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  peptide inhibitor of FoxP3

<400>  4
atgcgcgact ttcaaagttt ccgtaagatg tggccgtttt ttgcaatg                    48



<210>  5

<211>  495
<212>  PRT
<213>  Artificial sequence

<220>
<223>  anti-CD19 CAR

<400>  5
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Ile 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr 
        35                  40                  45              


Phe Thr Ser Tyr Val Met His Trp Val Lys Gln Lys Pro Gly Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr 
65                  70                  75                  80  


Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser 
                85                  90                  95      


Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg Val Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met 
145                 150                 155                 160 


Thr Gln Ala Ala Pro Ser Ile Pro Val Thr Pro Gly Glu Ser Val Ser 
                165                 170                 175     


Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Asn Ser Asn Gly Asn Thr 
            180                 185                 190         


Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu Leu 
        195                 200                 205             


Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg Ile Ser Arg Val Glu 
225                 230                 235                 240 


Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr Pro 
                245                 250                 255     


Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
305                 310                 315                 320 


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
                325                 330                 335     


Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
            340                 345                 350         


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
        355                 360                 365             


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
    370                 375                 380                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
385                 390                 395                 400 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                405                 410                 415     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            420                 425                 430         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495 


<210>  6

<211>  848
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Multi-chain CAR

<400>  6
Met Ala Pro Ala Met Glu Ser Pro Thr Leu Leu Cys Val Ala Leu Leu 
1               5                   10                  15      


Phe Phe Ala Pro Asp Gly Val Leu Ala Glu Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Ile Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Val Met His Trp Val Lys Gln 
    50                  55                  60                  


Lys Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn 
65                  70                  75                  80  


Asp Gly Thr Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr 
                85                  90                  95      


Ser Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Gly Thr Tyr Tyr Tyr 
        115                 120                 125             


Gly Ser Arg Val Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asp Ile Val Met Thr Gln Ala Ala Pro Ser Ile Pro Val Thr Pro 
                165                 170                 175     


Gly Glu Ser Val Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Asn 
            180                 185                 190         


Ser Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln 
        195                 200                 205             


Ser Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val 
    210                 215                 220                 


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg 
225                 230                 235                 240 


Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln 
                245                 250                 255     


His Leu Glu Tyr Pro Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            260                 265                 270         


Lys Arg Ala Asp Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        275                 280                 285             


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    290                 295                 300                 


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
305                 310                 315                 320 


Asp Phe Phe Ile Pro Leu Leu Val Val Ile Leu Phe Ala Val Asp Thr 
                325                 330                 335     


Gly Leu Phe Ile Ser Thr Gln Gln Gln Val Thr Phe Leu Leu Lys Ile 
            340                 345                 350         


Lys Arg Thr Arg Lys Gly Phe Arg Leu Leu Asn Pro His Pro Lys Pro 
        355                 360                 365             


Asn Pro Lys Asn Asn Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys 
    370                 375                 380                 


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Asp Thr Glu Ser Asn Arg 
385                 390                 395                 400 


Arg Ala Asn Leu Ala Leu Pro Gln Glu Pro Ser Ser Val Pro Ala Phe 
                405                 410                 415     


Glu Val Leu Glu Ile Ser Pro Gln Glu Val Ser Ser Gly Arg Leu Leu 
            420                 425                 430         


Lys Ser Ala Ser Ser Pro Pro Leu His Thr Trp Leu Thr Val Leu Lys 
        435                 440                 445             


Lys Glu Gln Glu Phe Leu Gly Val Thr Gln Ile Leu Thr Ala Met Ile 
    450                 455                 460                 


Cys Leu Cys Phe Gly Thr Val Val Cys Ser Val Leu Asp Ile Ser His 
465                 470                 475                 480 


Ile Glu Gly Asp Ile Phe Ser Ser Phe Lys Ala Gly Tyr Pro Phe Trp 
                485                 490                 495     


Gly Ala Ile Phe Phe Ser Ile Ser Gly Met Leu Ser Ile Ile Ser Glu 
            500                 505                 510         


Arg Arg Asn Ala Thr Tyr Leu Val Arg Gly Ser Leu Gly Ala Asn Thr 
        515                 520                 525             


Ala Ser Ser Ile Ala Gly Gly Thr Gly Ile Thr Ile Leu Ile Ile Asn 
    530                 535                 540                 


Leu Lys Lys Ser Leu Ala Tyr Ile His Ile His Ser Cys Gln Lys Phe 
545                 550                 555                 560 


Phe Glu Thr Lys Cys Phe Met Ala Ser Phe Ser Thr Glu Ile Val Val 
                565                 570                 575     


Met Met Leu Phe Leu Thr Ile Leu Gly Leu Gly Ser Ala Val Ser Leu 
            580                 585                 590         


Thr Ile Cys Gly Ala Gly Glu Glu Leu Lys Gly Asn Lys Val Pro Glu 
        595                 600                 605             


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
    610                 615                 620                 


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
625                 630                 635                 640 


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Ser Gly Val Lys Gln 
                645                 650                 655     


Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
            660                 665                 670         


Pro Gly Pro Met Ile Pro Ala Val Val Leu Leu Leu Leu Leu Leu Val 
        675                 680                 685             


Glu Gln Ala Ala Ala Leu Gly Glu Pro Gln Leu Cys Tyr Ile Leu Asp 
    690                 695                 700                 


Ala Ile Leu Phe Leu Tyr Gly Ile Val Leu Thr Leu Leu Tyr Cys Arg 
705                 710                 715                 720 


Leu Lys Ile Gln Val Arg Lys Ala Ala Ile Thr Ser Tyr Glu Lys Ser 
                725                 730                 735     


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
            740                 745                 750         


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
        755                 760                 765             


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
    770                 775                 780                 


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
785                 790                 795                 800 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
                805                 810                 815     


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
            820                 825                 830         


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        835                 840                 845             


<210>  7

<211>  2547
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multi-chain CAR

<400>  7
atggctcctg ccatggaatc ccctactcta ctgtgtgtag ccttactgtt cttcgctcca     60
gatggcgtgt tagcagaggt gcagttgcag cagtcagggc cagagttgat taagcccgga    120
gcctccgtca agatgtcctg caaggccagc gggtacactt tcaccagcta cgtcatgcat    180
tgggtgaagc agaagccagg ccaggggctt gagtggattg ggtacatcaa cccctacaac    240
gacgggacca aatacaacga gaaattcaag ggcaaagcca cactcacctc cgataagtcc    300
tcctctaccg cctacatgga gctcagctcc ctgacctccg aggatagcgc tgtgtattac    360
tgcgcaaggg gcacatacta ctatggctct agggtgttcg actactgggg gcagggcact    420
actctcacag tgagctcagg cggaggaggc agtggcggag ggggaagtgg gggcggcggc    480
agcgatattg tcatgaccca ggcagcccct agtatccctg tgactccagg cgagagcgtg    540
agcatcagct gccggtccag caagagcctg ctgaacagta acggaaacac atacctctac    600
tggtttctgc agaggcccgg ccagagccct cagctgctga tttaccgcat gtcaaatctt    660
gcctctgggg tgcccgatag atttagtggg agcggatccg gcacagcttt tacattgcgg    720
atctccagag tcgaggccga agacgtgggg gtctattact gtatgcaaca cctggaatac    780
ccctttacct tcggagccgg cacaaagctg gagctgaagc gggctgacac cacaaccccc    840
gctccaaggc cccctacccc cgcaccaact attgcctccc agccactctc actgcggcct    900
gaggcctgtc ggcccgctgc tggaggcgca gtgcatacaa ggggcctcga tttcgcctgc    960
gattttttta tcccattgtt ggtggtgatt ctgtttgctg tggacacagg attatttatc   1020
tcaactcagc agcaggtcac atttctcttg aagattaaga gaaccaggaa aggcttcaga   1080
cttctgaacc cacatcctaa gccaaacccc aaaaacaaca gagccgaggg cagaggcagc   1140
ctgctgacct gcggcgacgt ggaggagaac ccaggcccca tggacacaga aagtaatagg   1200
agagcaaatc ttgctctccc acaggagcct tccagtgtgc ctgcatttga agtcttggaa   1260
atatctcccc aggaagtatc ttcaggcaga ctattgaagt cggcctcatc cccaccactg   1320
catacatggc tgacagtttt gaaaaaagag caggagttcc tgggggtaac acaaattctg   1380
actgctatga tatgcctttg ttttggaaca gttgtctgct ctgtacttga tatttcacac   1440
attgagggag acattttttc atcatttaaa gcaggttatc cattctgggg agccatattt   1500
ttttctattt ctggaatgtt gtcaattata tctgaaagga gaaatgcaac atatctggtg   1560
agaggaagcc tgggagcaaa cactgccagc agcatagctg ggggaacggg aattaccatc   1620
ctgatcatca acctgaagaa gagcttggcc tatatccaca tccacagttg ccagaaattt   1680
tttgagacca agtgctttat ggcttccttt tccactgaaa ttgtagtgat gatgctgttt   1740
ctcaccattc tgggacttgg tagtgctgtg tcactcacaa tctgtggagc tggggaagaa   1800
ctcaaaggaa acaaggttcc agagaaacgg ggccggaaga agctcctcta catttttaag   1860
cagcctttca tgcggccagt gcagacaacc caagaggagg atgggtgttc ctgcagattc   1920
cctgaggaag aggaaggcgg gtgcgagctg ggttctggcg tgaaacagac tttgaatttt   1980
gaccttctca agttggcggg agacgtggag tccaacccag ggcccatgat tccagcagtg   2040
gtcttgctct tactcctttt ggttgaacaa gcagcggccc tgggagagcc tcagctctgc   2100
tatatcctgg atgccatcct gtttctgtat ggaattgtcc tcaccctcct ctactgtcga   2160
ctgaagatcc aagtgcgaaa ggcagctata accagctatg agaaatcaag agtgaagttc   2220
tccaggagcg cagatgcccc cgcctatcaa cagggccaga accagctcta caacgagctt   2280
aacctcggga ggcgcgaaga atacgacgtg ttggataaga gaagggggcg ggaccccgag   2340
atgggaggaa agccccggag gaagaaccct caggagggcc tgtacaacga gctgcagaag   2400
gataagatgg ccgaggccta ctcagagatc gggatgaagg gggagcggcg ccgcgggaag   2460
gggcacgatg ggctctacca ggggctgagc acagccacaa aggacacata cgacgccttg   2520
cacatgcagg cccttccacc ccggtga                                       2547


<210>  8
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD19 CAR

<400>  8

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Ile Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Val Met His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg Val Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ala Ala 
    130                 135                 140                 


Pro Ser Ile Pro Val Thr Pro Gly Glu Ser Val Ser Ile Ser Cys Arg 
145                 150                 155                 160 


Ser Ser Lys Ser Leu Leu Asn Ser Asn Gly Asn Thr Tyr Leu Tyr Trp 
                165                 170                 175     


Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Arg Met 
            180                 185                 190         


Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Ala Phe Thr Leu Arg Ile Ser Arg Val Glu Ala Glu Asp Val 
    210                 215                 220                 


Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr Pro Phe Thr Phe Gly 
225                 230                 235                 240 


Ala Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp 
                245                 250     


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scrambled p60

<400>  9

Met Lys Met Phe Phe Asp Ala Phe Pro Gln Arg Arg Ser Trp Phe 
1               5                   10                  15  


<210>  10
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  10

Gly Ser Ser Ser Ser 
1               5   


<210>  11
<211>  18
<212>  PRT
<213>  Gallus gallus

<400>  11

Met Arg Ser Leu Leu Ile Leu Val Leu Cys Phe Leu Pro Leu Ala Ala 
1               5                   10                  15      


Leu Gly 
        


<210>  12
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3R CLSP

<400>  12

Met Arg Arg Arg Ser Leu Leu Ile Leu Val Leu Cys Phe Leu Pro Leu 
1               5                   10                  15      


Ala Ala Leu Gly 
            20  


<210>  13
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4R CLSP

<400>  13

Met Arg Arg Arg Arg Ser Leu Leu Ile Leu Val Leu Cys Phe Leu Pro 
1               5                   10                  15      


Leu Ala Ala Leu Gly 
            20      


<210>  14
<211>  18
<212>  PRT
<213>  Homo sapiens

<400>  14

Met Lys Ala Leu Ile Val Leu Gly Leu Val Leu Leu Ser Val Thr Val 
1               5                   10                  15      


Gln Gly 
        


<210>  15
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  15

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser 
            20  


<210>  16
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3K HLSP

<400>  16

Met Lys Lys Lys Ala Leu Ile Val Leu Gly Leu Val Leu Leu Ser Val 
1               5                   10                  15      


Thr Val Gln Gly 
            20  


<210>  17
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3R IL2SP

<400>  17

Met Tyr Arg Arg Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu 
1               5                   10                  15      


Ala Leu Val Thr Asn Ser 
            20          






