                         SEQUENCE LISTING

<110>  DUKE UNIVERSITY
       EMORY UNIVERSITY
       CULLEN, Bryan
       KENNEDY, E. Matt
       KORNEPATI, Anand
       BOGERD, Hal
       MEFFERD, Adam
       SCHINAZI, Raymond
 
<120>  COMPOSITIONS FOR THE INACTIVATION OF VIRUS REPLICATION AND 
       METHODS OF MAKING AND USING THE SAME

<130>  5667-00184PCT

<150>  US 61/940,883
<151>  2014-02-18

<160>  68    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Hepatitis B Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Reverse Transcriptase YMDD

<400>  1
gttcagttat atggatgatg                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Hepatitis B Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Surface Antigen

<400>  2
gcctgtcctc caacttgtcc                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Hepatitis B Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Core

<400>  3
gtaccgcctc agctctgtat                                                   20


<210>  4
<211>  24
<212>  DNA
<213>  Hepatitis B Virus


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Reverse Transcriptase sgRNA-1

<400>  4
gctgggcttt cggaaaattc ctat                                              24


<210>  5
<211>  24
<212>  DNA
<213>  Hepatitis B Virus


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Surface Antigen sgRNA

<400>  5
ggctgaacat ggagaacatc acat                                              24


<210>  6
<211>  24
<212>  DNA
<213>  Hepatitis B Virus


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Core

<400>  6
gttataaaga atttggagct actg                                              24


<210>  7
<211>  24
<212>  DNA
<213>  Hepatitis B Virus


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Reverse Transcriptase sgRNA-2

<400>  7
gtgagtgatt ggaggttggg gact                                              24


<210>  8
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  E6 sgRNA 1

<400>  8
ggcgctttga ggatccaaca                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  E6 sgRNA 2

<400>  9
gaagctacct gatctgtgca                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  E7 sgRNA 1

<400>  10
ggagcaatta agcgactcag                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  E7 sgRNA 2

<400>  11
gaagaaaacg atgaaataga                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  HPV-16 E6t1

<400>  12
gcaacagtta ctgcgacgtg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  HPV-16 E7t1

<400>  13
gccagctgga caagcagaac                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  E6

<400>  14
gagcttgtag ggtcgccgtg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Human Papilloma Virus-18


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  E7

<400>  15
gcgtgacata gaaggtcaac                                                   20


<210>  16
<211>  24
<212>  DNA
<213>  Herpes Simplex Virus-1


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  ICP0 sgRNA 1

<400>  16
ggtccgtgct gtccgcctcg gagg                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Herpes Simplex Virus-1


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  ICP0 sgRNA 2

<400>  17
gaattgcatc caggttttca tgca                                              24


<210>  18
<211>  24
<212>  DNA
<213>  Herpes Simplex Virus-1


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  ICP4 sgRNA 1

<400>  18
ggtgggggtg gtcggggtcg tggt                                              24


<210>  19
<211>  24
<212>  DNA
<213>  Herpes Simplex Virus-1


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  ICP4 sgRNA 2

<400>  19
cgtgggggtg gtcggggtcg tggt                                              24


<210>  20
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Family of Repeats (FR)

<400>  20
gggaggtggc ggcatatgca                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Direct Symmetry (DS)

<400>  21
ttggtgtaag agcttcagcc                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  EBNA1 sgRNA-1

<400>  22
gtggacctca aagaagaggg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  EBNA1 sgRNA-2

<400>  23
agatgagggt gaggaagggc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Qp sgRNA-1

<400>  24
agaaattggg tgaccactga                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Qp sgRNA-2

<400>  25
ttgtctggtc gctagatggc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Latent Membrane Protein-1 sgRNA-1

<400>  26
gcttagctga actgggccgt                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Epstein-Barr Virus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Latent Membrane Protein-1 sgRNA-2

<400>  27
cttctgaaga taaagatgat                                                   20


<210>  28
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Bacterial targets used in tRNA analysis, P9 (Nme)

<400>  28
gacatcctca gatttagtat tcaga                                             25


<210>  29
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Bacterial targets used in tRNA analysis, P25 (Nme)

<400>  29
gtcatgcgcg gcgcattacc tttac                                             25


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HIV target sequence, TAR sgRNA 1

<400>  30
ggttagacca gatctgagcc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HIV target sequence, TAR sgRNA 2

<400>  31
gtctgagcct gggagctctc                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HIV target sequence, TAR sgRNA 3

<400>  32
ggagctccca ggctcagatc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HIV target sequence, TAT sgRNAs 1

<400>  33
gtagagccct ggaagcatcc                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HIV target sequence, TAT sgRNA  2

<400>  34
gagatcctag actagagccc                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: HIV target sequence, TAT sgRNA  3

<400>  35
ggctctagtc taggatctac                                                   20


<210>  36
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Spy sgRNA

<400>  36
gggtcttcga gaagacctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg       60

ttatcaactt gaaaaagtgg caccgagtcg gtgcttttt                              99


<210>  37
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nme sgRNA

<400>  37
gggtcttcga ggtctcagtt gtagctccca aacgaaatga gaaccgttgc tacaataagg       60

ccgtctgaaa agatgtgccg caacgctctg ccccttaaag cttctgcttt aaggggcatc      120

gtttattttt                                                             130


<210>  38
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sau sgRNA

<400>  38
ggagacggac gtctccgttt tagtactctg gaaacagaat ctactaaaac aaggcaaaat       60

gccgtgttta tctcgtcaac ttgttggcga gattttt                                97


<210>  39
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Variable sgRNA-1

<400>  39
ggagacggac gtctccgttt tagtactctg gaaacagaat ctactaaaac aaggcaaaat       60

gccgtgttta tctcgtcaac ttgttggcga gatttttt                               98


<210>  40
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Variable sgRNA-2

<400>  40
gggtcttcga gaagacccgt tttagtactc tggaaacaga atctactaaa acaaggcaaa       60

atgccgtgtt tatctcgtca acttgttggc gagatttttt                            100


<210>  41
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-99 (Gln)

<400>  41
ggttccatgg tgtaatggtt agcactctgg actctgaatc cagcgatccg agttcaaatc       60

tcggtggaac ct                                                           72


<210>  42
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-128 (Gly)

<400>  42
gcattggtgg ttcagtggta gaattctcgc ctgccacgcg ggaggcccgg gttcgattcc       60

cggccaatgc a                                                            71


<210>  43
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-115 (Asn)

<400>  43
tgtctctgtg gcgcaatcgg ttagcgcgtt cggctgttaa ctgaaaggtt agtggttcga       60

gcccacccgg ggacg                                                        75


<210>  44
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-7 (His)

<400>  44
gccgtgatcg tatagtggtt agtactctgc gttgtggccg cagcaacctc ggttcgaatc       60

cgagtcacgg ca                                                           72


<210>  45
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-49 (Gln-2)

<400>  45
ggttccatgg tgtaatggtt agcactctgg actctgaatc cagcgatccg agttcaaatc       60

tcggtggaac ct                                                           72


<210>  46
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-87 (Glu)

<400>  46
tccctggtgg tctagtggtt aggattcggc gctctcaccg ccgcggcccg ggttcgattc       60

ccggtcaggg aa                                                           72


<210>  47
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-2 (Pro)

<400>  47
ggctcgttgg tctaggggta tgattctcgc ttagggtgcg agaggtcccg ggttcaaatc       60

ccggacgagc cc                                                           72


<210>  48
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-25 (Cys)

<400>  48
gggggtatag ctcaggggta gagcatttga ctgcagatca agaggtcccc agttcaaatc       60

tgggtgcccc ct                                                           72


<210>  49
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: tRNA-5 (Tyr)

<400>  49
gtcagtgttg cacaacggtt aagtgaagag gctgtaaacc cagactggat gggttcaatt       60

cccatctctg ccg                                                          73


<210>  50
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: MHV tRNA-5

<400>  50
gccagggtag ctcaattggt agagcatcag gctagtatcc tgtcggttcc ggttcaagtc       60

cgggccctgg tt                                                           72


<210>  51
<211>  255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: U6 Promoter

<400>  51
tctagagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt       60

agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga      120

cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac      180

tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg      240

aaaggacgaa acacc                                                       255


<210>  52
<211>  298
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: EFS Promoter

<400>  52
ctcgaggtcg gaggatccac aaaggctagc cggtctatgc attaggtctt gaaaggagtg       60

ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag      120

ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg      180

gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata      240

agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagg        298


<210>  53
<211>  185
<212>  DNA
<213>  Rattus sp.


<220>
<221>  misc_feature
<222>  (1)..(185)
<223>  Rat Preproinsulin II Intron

<400>  53
aagcttggta agtgaccagc tacagtcgga aaccatcagc aagcaggtat gtactctcca       60

gggtgggcct ggcttcccca gtcaagactc cagggatttg agggacgctg tgggctcttc      120

tcttacatgt accttttgct agcctcaacc ctgactatct tccaggtcat tgttccaacg      180

ccacc                                                                  185


<210>  54
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: NLS-1x flag

<400>  54
atggcacttg aagctcccaa gaagaagcgg aaagtgggaa gcgattacaa ggatgacgat       60

gacaag                                                                  66


<210>  55
<211>  3159
<212>  DNA
<213>  S. aureus


<220>
<221>  misc_feature
<222>  (1)..(3159)
<223>  S. aureus Cas9

<400>  55
aagaggaatt acatcctcgg actggacatc ggcattacca gcgtcggcta cggcatcatc       60

gattacgaga caagggacgt gattgacgct ggcgtcaggc tgttcaagga ggccaacgtg      120

gagaataatg agggcaggag aagcaagagg ggcgccagga ggctgaagag gaggagaagg      180

cacagaatcc agagggtcaa gaagctcctg ttcgactaca acctgctgac cgaccatagc      240

gagctgagcg gcattaaccc ctacgaggct agggtcaaag gcctctccca gaaactgtcc      300

gaggaggagt ttagcgctgc tctcctgcac ctggccaaga ggaggggagt gcacaatgtg      360

aacgaggtcg aagaggacac cggcaatgag ctcagcacca aggagcagat tagcagaaac      420

agcaaagccc tggaggaaaa atatgtcgcc gagctacagc tggagagact caagaaggat      480

ggcgaggtca gaggctccat taacaggttc aagacctccg actacgtgaa agaagccaag      540

cagctgctga aggtgcaaaa ggcctatcat cagctggacc agagcttcat tgatacctat      600

atcgacctgc tcgaaaccag gagaacctac tatgagggcc ctggagaagg aagccccttc      660

ggatggaaag acattaagga gtggtacgaa atgctgatgg gacactgcac ctacttcccc      720

gaggagctga ggagcgtgaa atatgcctac aacgccgatc tctacaacgc cctgaacgac      780

ctgaacaatc tcgtgattac aagggatgag aacgaaaagc tggaatatta cgaaaaattt      840

cagatcattg aaaacgtgtt taagcaaaag aagaagccca ccctgaagca gattgccaag      900

gagatcctgg tgaacgaaga ggacattaaa ggctacagag tgacctccac aggcaaaccc      960

gaatttacca atctgaaggt gtatcacgat atcaaagata tcacagctag aaaggagatc     1020

atcgagaatg ctgagctgct cgaccaaatc gccaagattc tcaccatcta ccagagcagc     1080

gaggacatcc aggaagaact gacaaacctg aactccgagc tcacccagga agaaatcgag     1140

cagatttcca acctgaaagg ctacaccggc acccacaacc tgtccctgaa ggccatcaac     1200

ctgatcctcg atgaactctg gcacaccaat gacaatcaaa tcgctatctt caacagactg     1260

aagctcgtgc ccaagaaagt ggacctgagc cagcagaagg aaatccctac aaccctcgtg     1320

gacgatttca tcctcagccc cgtcgtgaag aggagcttca tccagtccat caaggtgatt     1380

aacgctatca tcaagaagta tggcctcccc aacgacatca tcatcgagct cgctagggaa     1440

aaaaactcca aggatgctca gaaaatgatc aacgagatgc agaaaaggaa cagacaaacc     1500

aacgaaagga ttgaggagat tatcaggacc accggcaagg agaatgctaa gtatctgatt     1560

gaaaaaatta aactccacga tatgcaggag ggcaagtgtc tctatagcct ggaagccatc     1620

cctctggagg atctcctgaa caatcctttt aactacgaag tggaccacat tatccccagg     1680

agcgtgtcct tcgataactc ctttaataat aaggtcctgg tcaaacagga ggagaactcc     1740

aagaagggaa acaggacacc cttccaatat ctctccagca gcgactccaa aataagctac     1800

gaaaccttta aaaaacacat tctgaacctg gccaagggca aaggcaggat cagcaaaacc     1860

aaaaaggaat acctcctgga ggagagagac atcaacaggt tctccgtgca gaaagacttc     1920

atcaacagga acctggtgga caccaggtat gccaccaggg gcctgatgaa cctcctgagg     1980

tcctatttta gagtcaataa tctcgacgtg aaagtcaaat ccattaatgg cggctttacc     2040

agcttcctga ggagaaaatg gaagttcaag aaggagagaa acaaaggcta caaacaccac     2100

gccgaagatg ccctcatcat tgctaacgcc gacttcatct ttaaggagtg gaagaagctc     2160

gataaggcca agaaagtcat ggagaatcaa atgttcgagg agaaacaagc cgaatccatg     2220

cccgagattg aaacggagca agagtacaag gaaatattca tcacccctca ccagatcaaa     2280

cacatcaagg acttcaaaga ctacaaatat agccatagag tcgataagaa gcccaacagg     2340

gagctgatca acgacacact ctattccacc agaaaggatg ataagggcaa caccctgatt     2400

gtgaacaacc tgaacggcct ctacgataag gataacgaca agctgaagaa gctgattaac     2460

aagagccccg agaaactgct catgtaccac cacgaccccc aaacatacca gaaactgaag     2520

ctgatcatgg aacagtatgg cgacgagaaa aaccccctgt ataagtacta tgaagaaacc     2580

ggcaactacc tcaccaagta cagcaaaaag gataacggac ccgtgatcaa aaagattaag     2640

tattacggca ataagctgaa tgcccacctc gatatcaccg atgattaccc caactccaga     2700

aataaagtcg tgaaactgtc cctgaaacct tacaggtttg acgtgtacct ggacaatggc     2760

gtgtacaagt tcgtgaccgt gaagaacctc gacgtgatta agaaggagaa ttactatgag     2820

gtcaacagca aatgctatga ggaagccaag aaactgaaga agatttccaa tcaggccgag     2880

ttcatcgcca gcttttataa caacgacctg attaagatca atggcgaact gtatagggtg     2940

atcggcgtca acaacgacct cctgaacagg attgaggtca atatgatcga tattacctac     3000

agagagtacc tcgaaaacat gaatgacaag aggcccccca ggattatcaa aaccatcgcc     3060

agcaagaccc agagcattaa gaaatacagc accgacatcc tcggcaacct gtacgaggtc     3120

aagtccaaaa agcaccccca gattatcaaa aagggatga                            3159


<210>  56
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Synthetic Poly (A)

<400>  56
gtcgacaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgt           56


<210>  57
<211>  1074
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: NLS-Flag-Sau Cas9 Protein

<400>  57

Met Ala Leu Glu Ala Pro Lys Lys Lys Arg Lys Val Gly Ser Asp Tyr 
1               5                   10                  15      


Lys Asp Asp Asp Asp Lys Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile 
            20                  25                  30          


Gly Ile Thr Ser Val Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp 
        35                  40                  45              


Val Ile Asp Ala Gly Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn 
    50                  55                  60                  


Asn Glu Gly Arg Arg Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg 
65                  70                  75                  80  


Arg Arg His Arg Ile Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn 
                85                  90                  95      


Leu Leu Thr Asp His Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala 
            100                 105                 110         


Arg Val Lys Gly Leu Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala 
        115                 120                 125             


Ala Leu Leu His Leu Ala Lys Arg Arg Gly Val His Asn Val Asn Glu 
    130                 135                 140                 


Val Glu Glu Asp Thr Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser 
145                 150                 155                 160 


Arg Asn Ser Lys Ala Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu 
                165                 170                 175     


Glu Arg Leu Lys Lys Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe 
            180                 185                 190         


Lys Thr Ser Asp Tyr Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln 
        195                 200                 205             


Lys Ala Tyr His Gln Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp 
    210                 215                 220                 


Leu Leu Glu Thr Arg Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser 
225                 230                 235                 240 


Pro Phe Gly Trp Lys Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly 
                245                 250                 255     


His Cys Thr Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr 
            260                 265                 270         


Asn Ala Asp Leu Tyr Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile 
        275                 280                 285             


Thr Arg Asp Glu Asn Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile 
    290                 295                 300                 


Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile 
305                 310                 315                 320 


Ala Lys Glu Ile Leu Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val 
                325                 330                 335     


Thr Ser Thr Gly Lys Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp 
            340                 345                 350         


Ile Lys Asp Ile Thr Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu 
        355                 360                 365             


Leu Asp Gln Ile Ala Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp 
    370                 375                 380                 


Ile Gln Glu Glu Leu Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu 
385                 390                 395                 400 


Ile Glu Gln Ile Ser Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu 
                405                 410                 415     


Ser Leu Lys Ala Ile Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn 
            420                 425                 430         


Asp Asn Gln Ile Ala Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys 
        435                 440                 445             


Val Asp Leu Ser Gln Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp 
    450                 455                 460                 


Phe Ile Leu Ser Pro Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys 
465                 470                 475                 480 


Val Ile Asn Ala Ile Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile 
                485                 490                 495     


Ile Glu Leu Ala Arg Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile 
            500                 505                 510         


Asn Glu Met Gln Lys Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu 
        515                 520                 525             


Ile Ile Arg Thr Thr Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys 
    530                 535                 540                 


Ile Lys Leu His Asp Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu 
545                 550                 555                 560 


Ala Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val 
                565                 570                 575     


Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn 
            580                 585                 590         


Lys Val Leu Val Lys Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr 
        595                 600                 605             


Pro Phe Gln Tyr Leu Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr 
    610                 615                 620                 


Phe Lys Lys His Ile Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser 
625                 630                 635                 640 


Lys Thr Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe 
                645                 650                 655     


Ser Val Gln Lys Asp Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr 
            660                 665                 670         


Ala Thr Arg Gly Leu Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn 
        675                 680                 685             


Asn Leu Asp Val Lys Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe 
    690                 695                 700                 


Leu Arg Arg Lys Trp Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys 
705                 710                 715                 720 


His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe 
                725                 730                 735     


Lys Glu Trp Lys Lys Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln 
            740                 745                 750         


Met Phe Glu Glu Lys Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu 
        755                 760                 765             


Gln Glu Tyr Lys Glu Ile Phe Ile Thr Pro His Gln Ile Lys His Ile 
    770                 775                 780                 


Lys Asp Phe Lys Asp Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro 
785                 790                 795                 800 


Asn Arg Glu Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp 
                805                 810                 815     


Lys Gly Asn Thr Leu Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys 
            820                 825                 830         


Asp Asn Asp Lys Leu Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu 
        835                 840                 845             


Leu Met Tyr His His Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile 
    850                 855                 860                 


Met Glu Gln Tyr Gly Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu 
865                 870                 875                 880 


Glu Thr Gly Asn Tyr Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro 
                885                 890                 895     


Val Ile Lys Lys Ile Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu 
            900                 905                 910         


Asp Ile Thr Asp Asp Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu 
        915                 920                 925             


Ser Leu Lys Pro Tyr Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr 
    930                 935                 940                 


Lys Phe Val Thr Val Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr 
945                 950                 955                 960 


Tyr Glu Val Asn Ser Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys 
                965                 970                 975     


Ile Ser Asn Gln Ala Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu 
            980                 985                 990         


Ile Lys Ile Asn Gly Glu Leu Tyr  Arg Val Ile Gly Val  Asn Asn Asp 
        995                 1000                 1005             


Leu Leu  Asn Arg Ile Glu Val  Asn Met Ile Asp Ile  Thr Tyr Arg 
    1010                 1015                 1020             


Glu Tyr  Leu Glu Asn Met Asn  Asp Lys Arg Pro Pro  Arg Ile Ile 
    1025                 1030                 1035             


Lys Thr  Ile Ala Ser Lys Thr  Gln Ser Ile Lys Lys  Tyr Ser Thr 
    1040                 1045                 1050             


Asp Ile  Leu Gly Asn Leu Tyr  Glu Val Lys Ser Lys  Lys His Pro 
    1055                 1060                 1065             


Gln Ile  Ile Lys Lys Gly 
    1070                 


<210>  58
<211>  4289
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: AAV construct insert (2 sgRNAs; synthetic)

<400>  58
ggttccatgg tgtaatggtt agcactctgg actctgaatc cagcgatccg agttcaaatc       60

tcggtggaac ctggagacgg acgtctccgt tttagtactc tggaaacaga atctactaaa      120

acaaggcaaa atgccgtgtt tatctcgtca acttgttggc gagatttttt tctagagagg      180

gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa      240

ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt      300

aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc      360

ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa      420

acaccgggtc ttcgagaaga cccgttttag tactctggaa acagaatcta ctaaaacaag      480

gcaaaatgcc gtgtttatct cgtcaacttg ttggcgagat tttttctcga ggtcggagga      540

tccacaaagg ctagccggtc tatgcattag gtcttgaaag gagtgggaat tggctccggt      600

gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc      660

ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg      720

tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc      780

gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggaagcttg gtaagtgacc      840

agctacagtc ggaaaccatc agcaagcagg tatgtactct ccagggtggg cctggcttcc      900

ccagtcaaga ctccagggat ttgagggacg ctgtgggctc ttctcttaca tgtacctttt      960

gctagcctca accctgacta tcttccaggt cattgttcca acgccaccat ggcacttgaa     1020

gctcccaaga agaagcggaa agtgggaagc gattacaagg atgacgatga caagaagagg     1080

aattacatcc tcggactgga catcggcatt accagcgtcg gctacggcat catcgattac     1140

gagacaaggg acgtgattga cgctggcgtc aggctgttca aggaggccaa cgtggagaat     1200

aatgagggca ggagaagcaa gaggggcgcc aggaggctga agaggaggag aaggcacaga     1260

atccagaggg tcaagaagct cctgttcgac tacaacctgc tgaccgacca tagcgagctg     1320

agcggcatta acccctacga ggctagggtc aaaggcctct cccagaaact gtccgaggag     1380

gagtttagcg ctgctctcct gcacctggcc aagaggaggg gagtgcacaa tgtgaacgag     1440

gtcgaagagg acaccggcaa tgagctcagc accaaggagc agattagcag aaacagcaaa     1500

gccctggagg aaaaatatgt cgccgagcta cagctggaga gactcaagaa ggatggcgag     1560

gtcagaggct ccattaacag gttcaagacc tccgactacg tgaaagaagc caagcagctg     1620

ctgaaggtgc aaaaggccta tcatcagctg gaccagagct tcattgatac ctatatcgac     1680

ctgctcgaaa ccaggagaac ctactatgag ggccctggag aaggaagccc cttcggatgg     1740

aaagacatta aggagtggta cgaaatgctg atgggacact gcacctactt ccccgaggag     1800

ctgaggagcg tgaaatatgc ctacaacgcc gatctctaca acgccctgaa cgacctgaac     1860

aatctcgtga ttacaaggga tgagaacgaa aagctggaat attacgaaaa atttcagatc     1920

attgaaaacg tgtttaagca aaagaagaag cccaccctga agcagattgc caaggagatc     1980

ctggtgaacg aagaggacat taaaggctac agagtgacct ccacaggcaa acccgaattt     2040

accaatctga aggtgtatca cgatatcaaa gatatcacag ctagaaagga gatcatcgag     2100

aatgctgagc tgctcgacca aatcgccaag attctcacca tctaccagag cagcgaggac     2160

atccaggaag aactgacaaa cctgaactcc gagctcaccc aggaagaaat cgagcagatt     2220

tccaacctga aaggctacac cggcacccac aacctgtccc tgaaggccat caacctgatc     2280

ctcgatgaac tctggcacac caatgacaat caaatcgcta tcttcaacag actgaagctc     2340

gtgcccaaga aagtggacct gagccagcag aaggaaatcc ctacaaccct cgtggacgat     2400

ttcatcctca gccccgtcgt gaagaggagc ttcatccagt ccatcaaggt gattaacgct     2460

atcatcaaga agtatggcct ccccaacgac atcatcatcg agctcgctag ggaaaaaaac     2520

tccaaggatg ctcagaaaat gatcaacgag atgcagaaaa ggaacagaca aaccaacgaa     2580

aggattgagg agattatcag gaccaccggc aaggagaatg ctaagtatct gattgaaaaa     2640

attaaactcc acgatatgca ggagggcaag tgtctctata gcctggaagc catccctctg     2700

gaggatctcc tgaacaatcc ttttaactac gaagtggacc acattatccc caggagcgtg     2760

tccttcgata actcctttaa taataaggtc ctggtcaaac aggaggagaa ctccaagaag     2820

ggaaacagga cacccttcca atatctctcc agcagcgact ccaaaataag ctacgaaacc     2880

tttaaaaaac acattctgaa cctggccaag ggcaaaggca ggatcagcaa aaccaaaaag     2940

gaatacctcc tggaggagag agacatcaac aggttctccg tgcagaaaga cttcatcaac     3000

aggaacctgg tggacaccag gtatgccacc aggggcctga tgaacctcct gaggtcctat     3060

tttagagtca ataatctcga cgtgaaagtc aaatccatta atggcggctt taccagcttc     3120

ctgaggagaa aatggaagtt caagaaggag agaaacaaag gctacaaaca ccacgccgaa     3180

gatgccctca tcattgctaa cgccgacttc atctttaagg agtggaagaa gctcgataag     3240

gccaagaaag tcatggagaa tcaaatgttc gaggagaaac aagccgaatc catgcccgag     3300

attgaaacgg agcaagagta caaggaaata ttcatcaccc ctcaccagat caaacacatc     3360

aaggacttca aagactacaa atatagccat agagtcgata agaagcccaa cagggagctg     3420

atcaacgaca cactctattc caccagaaag gatgataagg gcaacaccct gattgtgaac     3480

aacctgaacg gcctctacga taaggataac gacaagctga agaagctgat taacaagagc     3540

cccgagaaac tgctcatgta ccaccacgac ccccaaacat accagaaact gaagctgatc     3600

atggaacagt atggcgacga gaaaaacccc ctgtataagt actatgaaga aaccggcaac     3660

tacctcacca agtacagcaa aaaggataac ggacccgtga tcaaaaagat taagtattac     3720

ggcaataagc tgaatgccca cctcgatatc accgatgatt accccaactc cagaaataaa     3780

gtcgtgaaac tgtccctgaa accttacagg tttgacgtgt acctggacaa tggcgtgtac     3840

aagttcgtga ccgtgaagaa cctcgacgtg attaagaagg agaattacta tgaggtcaac     3900

agcaaatgct atgaggaagc caagaaactg aagaagattt ccaatcaggc cgagttcatc     3960

gccagctttt ataacaacga cctgattaag atcaatggcg aactgtatag ggtgatcggc     4020

gtcaacaacg acctcctgaa caggattgag gtcaatatga tcgatattac ctacagagag     4080

tacctcgaaa acatgaatga caagaggccc cccaggatta tcaaaaccat cgccagcaag     4140

acccagagca ttaagaaata cagcaccgac atcctcggca acctgtacga ggtcaagtcc     4200

aaaaagcacc cccagattat caaaaaggga tgagtcgaca ataaaatatc tttattttca     4260

ttacatctgt gtgttggttt tttgtgtgt                                       4289


<210>  59
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nuclear localization signal

<400>  59

Leu Glu Ala Pro Lys Lys Lys Arg Lys Val 
1               5                   10  


<210>  60
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nuclear localization signal

<400>  60

Ala Ala Pro Ala Ala Lys Lys Lys Leu Asp 
1               5                   10  


<210>  61
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nuclear localization signal

<400>  61

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  62
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nuclear localization signal

<400>  62

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  63
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ICP4 (Nme)

<400>  63
gcggtggccg gccgcgacgc cacg                                              24


<210>  64
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GFP (Nme)

<400>  64
gtcacgaggg tgggccaggg cacg                                              24


<210>  65
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G6P (Nme)

<400>  65
gatctggttc catcttaaag agact                                             25


<210>  66
<211>  23
<212>  DNA
<213>  Human papilloma virus


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  Wild-type E6 partial sequence

<400>  66
cgcgctttga ggatccaaca cgg                                               23


<210>  67
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Mutant E6 partial sequence

<400>  67
cgcgctttga ggaccccacg cgg                                               23


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: oligonucleotide

<400>  68
gaaatcctgc agaaagacct                                                   20


