                         SEQUENCE LISTING

<110>  The Wistar Institute of Anatomy and Biology
       The Trustees of the University of Pennsylvania
 
<120>  SMALL MOLECULE HSP70 INHIBITORS

<130>  UPN-14-7001PCT

<150>  61/944,798
<151>  2014-02-26

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  1926
<212>  DNA
<213>  Homo sapiens

<400>  1
atggccaaag ccgcggcgat cggcatcgac ctgggcacca cctactcctg cgtgggggtg       60

ttccaacacg gcaaggtgga gatcatcgcc aacgaccagg gcaaccgcac cacccccagc      120

tacgtggcct tcacggacac cgagcggctc atcggggatg cggccaagaa ccaggtggcg      180

ctgaacccgc agaacaccgt gtttgacgcg aagcggctga tcggccgcaa gttcggcgac      240

ccggtggtgc agtcggacat gaagcactgg cctttccagg tgatcaacga cggagacaag      300

cccaaggtgc aggtgagcta caagggggac accaaggcat tctaccccga ggagatctcg      360

tccatggtgc tgaccaagat gaaggagatc gccgaggcgt acctgggcta cccggtgacc      420

aacgcggtga tcaccgtgcc ggcctacttc aacgactcgc agcgccaggc caccaaggat      480

gcgggtgtga tcgcggggct caacgtgctg cggatcatca acgagcccac ggccgccgcc      540

atcgcctacg gcctggacag aacgggcaag ggggagcgca acgtgctcat ctttgacctg      600

ggcgggggca ccttcgacgt gtccatcctg acgatcgacg acggcatctt cgaggtgaag      660

gccacggccg gggacaccca cctgggtggg gaggactttg acaacaggct ggtgaaccac      720

ttcgtggagg agttcaagag aaaacacaag aaggacatca gccagaacaa gcgagccgtg      780

aggcggctgc gcaccgcctg cgagagggcc aagaggaccc tgtcgtccag cacccaggcc      840

agcctggaga tcgactccct gtttgagggc atcgacttct acacgtccat caccagggcg      900

aggttcgagg agctgtgctc cgacctgttc cgaagcaccc tggagcccgt ggagaaggct      960

ctgcgcgacg ccaagctgga caaggcccag attcacgacc tggtcctggt cgggggctcc     1020

acccgcatcc ccaaggtgca gaagctgctg caggacttct tcaacgggcg cgacctgaac     1080

aagagcatca accccgacga ggctgtggcc tacggggcgg cggtgcaggc ggccatcctg     1140

atgggggaca agtccgagaa cgtgcaggac ctgctgctgc tggacgtggc tcccctgtcg     1200

ctggggctgg agacggccgg aggcgtgatg actgccctga tcaagcgcaa ctccaccatc     1260

cccaccaagc agacgcagat cttcaccacc tactccgaca accaacccgg ggtgctgatc     1320

caggtgtacg agggcgagag ggccatgacg aaagacaaca atctgttggg gcgcttcgag     1380

ctgagcggca tccctccggc ccccaggggc gtgccccaga tcgaggtgac cttcgacatc     1440

gatgccaacg gcatcctgaa cgtcacggcc acggacaaga gcaccggcaa ggccaacaag     1500

atcaccatca ccaacgacaa gggccgcctg agcaaggagg agatcgagcg catggtgcag     1560

gaggcggaga agtacaaagc ggaggacgag gtgcagcgcg agagggtgtc agccaagaac     1620

gccctggagt cctacgcctt caacatgaag agcgccgtgg aggatgaggg gctcaagggc     1680

aagatcagcg aggccgacaa gaagaaggtg ctggacaagt gtcaagaggt catctcgtgg     1740

ctggacgcca acaccttggc cgagaaggac gagtttgagc acaagaggaa ggagctggag     1800

caggtgtgta accccatcat cagcggactg taccagggtg ccggtggtcc cgggcctggg     1860

ggcttcgggg ctcagggtcc caagggaggg tctgggtcag gccccaccat tgaggaggta     1920

gattag                                                                1926


<210>  2
<211>  641
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Lys Ala Ala Ala Ile Gly Ile Asp Leu Gly Thr Thr Tyr Ser 
1               5                   10                  15      


Cys Val Gly Val Phe Gln His Gly Lys Val Glu Ile Ile Ala Asn Asp 
            20                  25                  30          


Gln Gly Asn Arg Thr Thr Pro Ser Tyr Val Ala Phe Thr Asp Thr Glu 
        35                  40                  45              


Arg Leu Ile Gly Asp Ala Ala Lys Asn Gln Val Ala Leu Asn Pro Gln 
    50                  55                  60                  


Asn Thr Val Phe Asp Ala Lys Arg Leu Ile Gly Arg Lys Phe Gly Asp 
65                  70                  75                  80  


Pro Val Val Gln Ser Asp Met Lys His Trp Pro Phe Gln Val Ile Asn 
                85                  90                  95      


Asp Gly Asp Lys Pro Lys Val Gln Val Ser Tyr Lys Gly Asp Thr Lys 
            100                 105                 110         


Ala Phe Tyr Pro Glu Glu Ile Ser Ser Met Val Leu Thr Lys Met Lys 
        115                 120                 125             


Glu Ile Ala Glu Ala Tyr Leu Gly Tyr Pro Val Thr Asn Ala Val Ile 
    130                 135                 140                 


Thr Val Pro Ala Tyr Phe Asn Asp Ser Gln Arg Gln Ala Thr Lys Asp 
145                 150                 155                 160 


Ala Gly Val Ile Ala Gly Leu Asn Val Leu Arg Ile Ile Asn Glu Pro 
                165                 170                 175     


Thr Ala Ala Ala Ile Ala Tyr Gly Leu Asp Arg Thr Gly Lys Gly Glu 
            180                 185                 190         


Arg Asn Val Leu Ile Phe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser 
        195                 200                 205             


Ile Leu Thr Ile Asp Asp Gly Ile Phe Glu Val Lys Ala Thr Ala Gly 
    210                 215                 220                 


Asp Thr His Leu Gly Gly Glu Asp Phe Asp Asn Arg Leu Val Asn His 
225                 230                 235                 240 


Phe Val Glu Glu Phe Lys Arg Lys His Lys Lys Asp Ile Ser Gln Asn 
                245                 250                 255     


Lys Arg Ala Val Arg Arg Leu Arg Thr Ala Cys Glu Arg Ala Lys Arg 
            260                 265                 270         


Thr Leu Ser Ser Ser Thr Gln Ala Ser Leu Glu Ile Asp Ser Leu Phe 
        275                 280                 285             


Glu Gly Ile Asp Phe Tyr Thr Ser Ile Thr Arg Ala Arg Phe Glu Glu 
    290                 295                 300                 


Leu Cys Ser Asp Leu Phe Arg Ser Thr Leu Glu Pro Val Glu Lys Ala 
305                 310                 315                 320 


Leu Arg Asp Ala Lys Leu Asp Lys Ala Gln Ile His Asp Leu Val Leu 
                325                 330                 335     


Val Gly Gly Ser Thr Arg Ile Pro Lys Val Gln Lys Leu Leu Gln Asp 
            340                 345                 350         


Phe Phe Asn Gly Arg Asp Leu Asn Lys Ser Ile Asn Pro Asp Glu Ala 
        355                 360                 365             


Val Ala Tyr Gly Ala Ala Val Gln Ala Ala Ile Leu Met Gly Asp Lys 
    370                 375                 380                 


Ser Glu Asn Val Gln Asp Leu Leu Leu Leu Asp Val Ala Pro Leu Ser 
385                 390                 395                 400 


Leu Gly Leu Glu Thr Ala Gly Gly Val Met Thr Ala Leu Ile Lys Arg 
                405                 410                 415     


Asn Ser Thr Ile Pro Thr Lys Gln Thr Gln Ile Phe Thr Thr Tyr Ser 
            420                 425                 430         


Asp Asn Gln Pro Gly Val Leu Ile Gln Val Tyr Glu Gly Glu Arg Ala 
        435                 440                 445             


Met Thr Lys Asp Asn Asn Leu Leu Gly Arg Phe Glu Leu Ser Gly Ile 
    450                 455                 460                 


Pro Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val Thr Phe Asp Ile 
465                 470                 475                 480 


Asp Ala Asn Gly Ile Leu Asn Val Thr Ala Thr Asp Lys Ser Thr Gly 
                485                 490                 495     


Lys Ala Asn Lys Ile Thr Ile Thr Asn Asp Lys Gly Arg Leu Ser Lys 
            500                 505                 510         


Glu Glu Ile Glu Arg Met Val Gln Glu Ala Glu Lys Tyr Lys Ala Glu 
        515                 520                 525             


Asp Glu Val Gln Arg Glu Arg Val Ser Ala Lys Asn Ala Leu Glu Ser 
    530                 535                 540                 


Tyr Ala Phe Asn Met Lys Ser Ala Val Glu Asp Glu Gly Leu Lys Gly 
545                 550                 555                 560 


Lys Ile Ser Glu Ala Asp Lys Lys Lys Val Leu Asp Lys Cys Gln Glu 
                565                 570                 575     


Val Ile Ser Trp Leu Asp Ala Asn Thr Leu Ala Glu Lys Asp Glu Phe 
            580                 585                 590         


Glu His Lys Arg Lys Glu Leu Glu Gln Val Cys Asn Pro Ile Ile Ser 
        595                 600                 605             


Gly Leu Tyr Gln Gly Ala Gly Gly Pro Gly Pro Gly Gly Phe Gly Ala 
    610                 615                 620                 


Gln Gly Pro Lys Gly Gly Ser Gly Ser Gly Pro Thr Ile Glu Glu Val 
625                 630                 635                 640 


Asp 
    


<210>  3
<211>  1917
<212>  DNA
<213>  Escherchia coli

<400>  3
atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat       60

ggcaccactc ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc ttctatcatt      120

gcctataccc aggatggtga aactctagtt ggtcagccgg ctaaacgtca ggcagtgacg      180

aacccgcaaa acactctgtt tgcgattaaa cgcctgattg gtcgccgctt ccaggacgaa      240

gaagtacagc gtgatgtttc catcatgccg ttcaaaatta ttgctgctga taacggcgac      300

gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatttctgc tgaagtgctg      360

aaaaaaatga agaaaaccgc tgaagattac ctgggtgaac cggtaactga agctgttatc      420

accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc      480

gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt      540

ctggacaaag gcactggcaa ccgtactatc gcggtttatg acctgggtgg tggtactttc      600

gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga agttctggca      660

accaacggtg atacccacct ggggggtgaa gacttcgaca gccgtctgat caactatctg      720

gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct ggcaatgcag      780

cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac      840

gttaacctgc catacatcac tgcagacgcg accggtccga aacacatgaa catcaaagtg      900

actcgtgcga aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg      960

aaagttgcac tgcaggacgc tggcctgtcc gtatctgata tcgacgacgt tatcctcgtt     1020

ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg ctgagttctt tggtaaagag     1080

ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt tcagggtggt     1140

gttctgactg gtgacgtaaa agacgtactg ctgctggacg ttaccccgct gtctctgggt     1200

atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac tatcccgacc     1260

aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg     1320

ctgcagggtg aacgtaaacg tgcggctgat aacaaatctc tgggtcagtt caacctagat     1380

ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag ttaccttcga tatcgatgct     1440

gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca gaagatcacc     1500

atcaaggctt cttctggtct gaacgaagat gaaatccaga aaatggtacg cgacgcagaa     1560

gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa ccagggcgac     1620

catctgctgc acagcacccg taagcaggtt gaagaagcag gcgacaaact gccggctgac     1680

gacaaaactg ctatcgagtc tgcgctgact gcactggaaa ctgctctgaa aggtgaagac     1740

aaagccgcta tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa actgatggaa     1800

atcgcccagc agcaacatgc ccagcagcag actgccggtg ctgatgcttc tgcaaacaac     1860

gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag tcaaagacaa aaaataa        1917


<210>  4
<211>  638
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Gly Lys Ile Ile Gly Ile Asp Leu Gly Thr Thr Asn Ser Cys Val 
1               5                   10                  15      


Ala Ile Met Asp Gly Thr Thr Pro Arg Val Leu Glu Asn Ala Glu Gly 
            20                  25                  30          


Asp Arg Thr Thr Pro Ser Ile Ile Ala Tyr Thr Gln Asp Gly Glu Thr 
        35                  40                  45              


Leu Val Gly Gln Pro Ala Lys Arg Gln Ala Val Thr Asn Pro Gln Asn 
    50                  55                  60                  


Thr Leu Phe Ala Ile Lys Arg Leu Ile Gly Arg Arg Phe Gln Asp Glu 
65                  70                  75                  80  


Glu Val Gln Arg Asp Val Ser Ile Met Pro Phe Lys Ile Ile Ala Ala 
                85                  90                  95      


Asp Asn Gly Asp Ala Trp Val Glu Val Lys Gly Gln Lys Met Ala Pro 
            100                 105                 110         


Pro Gln Ile Ser Ala Glu Val Leu Lys Lys Met Lys Lys Thr Ala Glu 
        115                 120                 125             


Asp Tyr Leu Gly Glu Pro Val Thr Glu Ala Val Ile Thr Val Pro Ala 
    130                 135                 140                 


Tyr Phe Asn Asp Ala Gln Arg Gln Ala Thr Lys Asp Ala Gly Arg Ile 
145                 150                 155                 160 


Ala Gly Leu Glu Val Lys Arg Ile Ile Asn Glu Pro Thr Ala Ala Ala 
                165                 170                 175     


Leu Ala Tyr Gly Leu Asp Lys Gly Thr Gly Asn Arg Thr Ile Ala Val 
            180                 185                 190         


Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ile Ser Ile Ile Glu Ile Asp 
        195                 200                 205             


Glu Val Asp Gly Glu Lys Thr Phe Glu Val Leu Ala Thr Asn Gly Asp 
    210                 215                 220                 


Thr His Leu Gly Gly Glu Asp Phe Asp Ser Arg Leu Ile Asn Tyr Leu 
225                 230                 235                 240 


Val Glu Glu Phe Lys Lys Asp Gln Gly Ile Asp Leu Arg Asn Asp Pro 
                245                 250                 255     


Leu Ala Met Gln Arg Leu Lys Glu Ala Ala Glu Lys Ala Lys Ile Glu 
            260                 265                 270         


Leu Ser Ser Ala Gln Gln Thr Asp Val Asn Leu Pro Tyr Ile Thr Ala 
        275                 280                 285             


Asp Ala Thr Gly Pro Lys His Met Asn Ile Lys Val Thr Arg Ala Lys 
    290                 295                 300                 


Leu Glu Ser Leu Val Glu Asp Leu Val Asn Arg Ser Ile Glu Pro Leu 
305                 310                 315                 320 


Lys Val Ala Leu Gln Asp Ala Gly Leu Ser Val Ser Asp Ile Asp Asp 
                325                 330                 335     


Val Ile Leu Val Gly Gly Gln Thr Arg Met Pro Met Val Gln Lys Lys 
            340                 345                 350         


Val Ala Glu Phe Phe Gly Lys Glu Pro Arg Lys Asp Val Asn Pro Asp 
        355                 360                 365             


Glu Ala Val Ala Ile Gly Ala Ala Val Gln Gly Gly Val Leu Thr Gly 
    370                 375                 380                 


Asp Val Lys Asp Val Leu Leu Leu Asp Val Thr Pro Leu Ser Leu Gly 
385                 390                 395                 400 


Ile Glu Thr Met Gly Gly Val Met Thr Thr Leu Ile Ala Lys Asn Thr 
                405                 410                 415     


Thr Ile Pro Thr Lys His Ser Gln Val Phe Ser Thr Ala Glu Asp Asn 
            420                 425                 430         


Gln Ser Ala Val Thr Ile His Val Leu Gln Gly Glu Arg Lys Arg Ala 
        435                 440                 445             


Ala Asp Asn Lys Ser Leu Gly Gln Phe Asn Leu Asp Gly Ile Asn Pro 
    450                 455                 460                 


Ala Pro Arg Gly Met Pro Gln Ile Glu Val Thr Phe Asp Ile Asp Ala 
465                 470                 475                 480 


Asp Gly Ile Leu His Val Ser Ala Lys Asp Lys Asn Ser Gly Lys Glu 
                485                 490                 495     


Gln Lys Ile Thr Ile Lys Ala Ser Ser Gly Leu Asn Glu Asp Glu Ile 
            500                 505                 510         


Gln Lys Met Val Arg Asp Ala Glu Ala Asn Ala Glu Ala Asp Arg Lys 
        515                 520                 525             


Phe Glu Glu Leu Val Gln Thr Arg Asn Gln Gly Asp His Leu Leu His 
    530                 535                 540                 


Ser Thr Arg Lys Gln Val Glu Glu Ala Gly Asp Lys Leu Pro Ala Asp 
545                 550                 555                 560 


Asp Lys Thr Ala Ile Glu Ser Ala Leu Thr Ala Leu Glu Thr Ala Leu 
                565                 570                 575     


Lys Gly Glu Asp Lys Ala Ala Ile Glu Ala Lys Met Gln Glu Leu Ala 
            580                 585                 590         


Gln Val Ser Gln Lys Leu Met Glu Ile Ala Gln Gln Gln His Ala Gln 
        595                 600                 605             


Gln Gln Thr Ala Gly Ala Asp Ala Ser Ala Asn Asn Ala Lys Asp Asp 
    610                 615                 620                 


Asp Val Val Asp Ala Glu Phe Glu Glu Val Lys Asp Lys Lys 
625                 630                 635             


<210>  5
<211>  230
<212>  PRT
<213>  Escherichia coli

<400>  5

Met His His His His His His Ile Glu Gly Arg Val Leu Leu Leu Asp 
1               5                   10                  15      


Val Thr Pro Leu Ser Leu Gly Ile Glu Thr Met Gly Gly Val Met Thr 
            20                  25                  30          


Thr Leu Ile Ala Lys Asn Thr Thr Ile Pro Thr Lys His Ser Gln Val 
        35                  40                  45              


Phe Ser Thr Ala Glu Asp Asn Gln Ser Ala Val Thr Ile His Val Leu 
    50                  55                  60                  


Gln Gly Glu Arg Lys Arg Ala Ala Asp Asn Lys Ser Leu Gly Gln Phe 
65                  70                  75                  80  


Asn Leu Asp Gly Ile Asn Pro Ala Pro Arg Gly Met Pro Gln Ile Glu 
                85                  90                  95      


Val Thr Phe Asp Ile Asp Ala Asp Gly Ile Leu His Val Ser Ala Lys 
            100                 105                 110         


Asp Lys Asn Ser Gly Lys Glu Gln Lys Ile Thr Ile Lys Ala Ser Ser 
        115                 120                 125             


Gly Leu Asn Glu Asp Glu Ile Gln Lys Met Val Arg Asp Ala Glu Ala 
    130                 135                 140                 


Asn Ala Glu Ala Asp Arg Lys Phe Glu Glu Leu Val Gln Thr Arg Asn 
145                 150                 155                 160 


Gln Gly Asp His Leu Leu His Ser Thr Arg Lys Gln Val Glu Glu Ala 
                165                 170                 175     


Gly Asp Lys Leu Pro Ala Asp Asp Lys Thr Ala Ile Glu Ser Ala Leu 
            180                 185                 190         


Thr Ala Leu Glu Thr Ala Leu Lys Gly Glu Asp Lys Ala Ala Ile Glu 
        195                 200                 205             


Ala Lys Met Gln Glu Leu Ala Gln Val Ser Gln Lys Leu Met Glu Ile 
    210                 215                 220                 


Ala Gln Gln Gln His Ala 
225                 230 


<210>  6
<211>  238
<212>  PRT
<213>  Homo sapiens

<400>  6

Met His His His His His His Glu Asn Val Gln Asp Leu Leu Leu Leu 
1               5                   10                  15      


Asp Val Ala Pro Leu Ser Leu Gly Leu Glu Thr Ala Gly Gly Val Met 
            20                  25                  30          


Thr Ala Leu Ile Lys Arg Asn Ser Thr Ile Pro Thr Lys Gln Thr Gln 
        35                  40                  45              


Ile Phe Thr Thr Tyr Ser Asp Asn Gln Pro Gly Val Leu Ile Gln Val 
    50                  55                  60                  


Tyr Glu Gly Glu Arg Ala Met Thr Lys Asp Asn Asn Leu Leu Gly Arg 
65                  70                  75                  80  


Phe Glu Leu Ser Gly Ile Pro Pro Ala Pro Arg Gly Val Pro Gln Ile 
                85                  90                  95      


Glu Val Thr Phe Asp Ile Asp Ala Asn Gly Ile Leu Asn Val Thr Ala 
            100                 105                 110         


Thr Asp Lys Ser Thr Gly Lys Ala Asn Lys Ile Thr Ile Thr Asn Asp 
        115                 120                 125             


Lys Gly Arg Leu Ser Lys Glu Glu Ile Glu Arg Met Val Gln Glu Ala 
    130                 135                 140                 


Glu Lys Tyr Lys Ala Glu Asp Glu Val Gln Arg Glu Arg Val Ser Ala 
145                 150                 155                 160 


Lys Asn Ala Leu Glu Ser Tyr Ala Phe Asn Met Lys Ser Ala Val Glu 
                165                 170                 175     


Asp Glu Gly Leu Lys Gly Lys Ile Ser Glu Ala Asp Lys Lys Lys Val 
            180                 185                 190         


Leu Asp Lys Cys Gln Glu Val Ile Ser Trp Leu Asp Ala Asn Thr Leu 
        195                 200                 205             


Ala Glu Lys Asp Glu Phe Glu His Lys Arg Lys Glu Leu Glu Gln Val 
    210                 215                 220                 


Cys Asn Pro Ile Ile Ser Gly Leu Tyr Gln Gly Ala Gly Gly 
225                 230                 235             


<210>  7
<211>  7
<212>  PRT
<213>  Escherichia coli

<400>  7

Asn Arg Leu Leu Leu Thr Gly 
1               5           


