               SEQUENCE LISTING

<110> Academia Sinica
      Kaohsiung Medical University
      Roffler, Steven R. 

<120> BI-SPECIFIC ANTIBODIES AND USES THEREOF

<130> P2862-PCT

<150> US61/946,997
<151> 2014-03-03
<150> US61/946,980
<151> 2014-03-03

<160> 215

<170> BiSSAP 1.3

<210> 1
<211> 219
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized E11 VL-C kappa

<400> 1
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Lys Ser Ile Val His Ser 
            20                  25                  30          
Asn Gly Asn Thr Tyr Leu Glu Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              
Pro Arg Arg Leu Ile Tyr Lys Val Ser Lys Arg Met Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Gly 
                85                  90                  95      
Ser His Val Pro Pro Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         
Lys His Lys Leu Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 

<210> 2
<211> 217
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized E11 VH-CH1

<400> 2
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          
Thr Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              
Gly Tyr Ile Ile Pro Ser Ser Gly Tyr Val Asp Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Val Arg Ser Leu Asp Gly Tyr Phe Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Arg Val 
    210                 215         

<210> 3
<211> 232
<212> PRT
<213> Artificial Sequence

<220> 
<223> Hinge CH2-CH3

<400> 3
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 
Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         

<210> 4
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> Peptide Linker

<400> 4
Val Asp Leu Val Thr Val Ser Ser Ala Ser Thr Gly Gly Gly Ser Gly 
1               5                   10                  15      
Gln Leu Gly Gly Gly Gly Ser 
            20              

<210> 5
<211> 243
<212> PRT
<213> Artificial Sequence

<220> 
<223> Hcc49 dsFv

<400> 5
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          
Ala Ile His Trp Val Arg Gln Ala Pro Gly Gln Cys Leu Glu Trp Met 
        35                  40                  45              
Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser Gln Lys Phe 
    50                  55                  60                  
Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Ser Trp Ile Met Gln Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             
Gly Ser Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser 
    130                 135                 140                 
Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu 
145                 150                 155                 160 
Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     
Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser 
            180                 185                 190         
Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             
Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys 
    210                 215                 220                 
Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Cys Gly Thr Lys Val 
225                 230                 235                 240 
Glu Ile Lys 
            

<210> 6
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> 6xHis Tag

<400> 6
Thr Arg His His His His His His 
1               5               

<210> 7
<211> 243
<212> PRT
<213> Artificial Sequence

<220> 
<223> 11F8 anti-EGFR dsFv

<400> 7
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Gly 
            20                  25                  30          
Asp Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Cys Leu Glu 
        35                  40                  45              
Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asp Tyr Asn Pro Ser 
    50                  55                  60                  
Leu Lys Ser Arg Val Thr Met Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Val Asn Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      
Cys Ala Arg Val Ser Ile Phe Gly Val Gly Thr Phe Asp Tyr Trp Gly 
            100                 105                 110         
Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             
Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Met Thr Gln Ser Pro 
    130                 135                 140                 
Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
145                 150                 155                 160 
Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     
Gly Gln Ala Pro Arg Leu Leu Ile Tyr Asp Ala Ser Asn Arg Ala Thr 
            180                 185                 190         
Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             
Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys 
    210                 215                 220                 
His Gln Tyr Gly Ser Thr Pro Leu Thr Phe Gly Cys Gly Thr Lys Ala 
225                 230                 235                 240 
Glu Ile Lys 
            

<210> 8
<211> 243
<212> PRT
<213> Artificial Sequence

<220> 
<223> C6ML3-9 anti-HER2 dsFv

<400> 8
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Gly 
            20                  25                  30          
Asp Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Cys Leu Glu 
        35                  40                  45              
Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asp Tyr Asn Pro Ser 
    50                  55                  60                  
Leu Lys Ser Arg Val Thr Met Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Val Asn Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      
Cys Ala Arg Val Ser Ile Phe Gly Val Gly Thr Phe Asp Tyr Trp Gly 
            100                 105                 110         
Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             
Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Met Thr Gln Ser Pro 
    130                 135                 140                 
Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
145                 150                 155                 160 
Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     
Gly Gln Ala Pro Arg Leu Leu Ile Tyr Asp Ala Ser Asn Arg Ala Thr 
            180                 185                 190         
Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             
Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys 
    210                 215                 220                 
His Gln Tyr Gly Ser Thr Pro Leu Thr Phe Gly Cys Gly Thr Lys Ala 
225                 230                 235                 240 
Glu Ile Lys 
            

<210> 9
<211> 155
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized 6.3 VL-Ckappa

<400> 9
Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          
Ser Asn Gln Met Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Leu Gln 
                85                  90                  95      
Tyr Leu Ser Ser Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
        115                 120                 125             
Lys His Lys Leu Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
    130                 135                 140                 
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
145                 150                 155 

<210> 10
<211> 219
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized 6.3 VH-CH1

<400> 10
Gln Val Gln Leu Val Gln Ser Gly Ser Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Lys Asn Tyr 
            20                  25                  30          
Gly Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              
Gly Trp Ile Asn Thr Tyr Thr Gly Gln Pro Ile Tyr Ala Asn Asp Phe 
    50                  55                  60                  
Lys Gly Arg Phe Val Phe Ser Leu Asp Thr Ser Val Ser Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Ile Ser Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Asp Trp Gly Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         
Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
        115                 120                 125             
Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys 
    130                 135                 140                 
Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
145                 150                 155                 160 
Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
                165                 170                 175     
Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
            180                 185                 190         
Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
        195                 200                 205             
Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 

<210> 11
<211> 242
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv

<400> 11
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Thr Ser 
            20                  25                  30          
Gly Met Gly Val Gly Trp Ile Arg Gln His Pro Gly Lys Cys Leu Glu 
        35                  40                  45              
Trp Ile Gly His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala 
    50                  55                  60                  
Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      
Cys Ala Arg Met Glu Leu Trp Ser Tyr Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala 
    130                 135                 140                 
Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Ser Ala 
145                 150                 155                 160 
Ser Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln 
                165                 170                 175     
Ala Pro Arg Leu Leu Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Ile 
            180                 185                 190         
Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        195                 200                 205             
Ile Ser Ser Leu Glu Pro Glu Asp Val Ala Val Tyr Tyr Cys Phe Gln 
    210                 215                 220                 
Gly Ser Val Tyr Pro Phe Thr Phe Gly Cys Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 
Lys Arg 
        

<210> 12
<211> 214
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized 15-2b VL-Ckappa

<400> 12
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Asn Thr Ser 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Ile Ala Thr Tyr Tyr Cys Leu Gln Tyr Ile Asn Tyr Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         
Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             
Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 
Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 
Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     
Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Leu Tyr 
            180                 185                 190         
Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             
Phe Asn Arg Gly Glu Cys 
    210                 

<210> 13
<211> 215
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized 15-2b VH-CH1

<400> 13
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          
Trp Met Asn Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Gly Glu Ile Arg Ser Lys Ser Asn Asn Tyr Ala Thr His Tyr Ala Glu 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  
Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ser Asn Arg Tyr Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         
Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys 
        115                 120                 125             
Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys 
    130                 135                 140                 
Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
145                 150                 155                 160 
Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
                165                 170                 175     
Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr 
            180                 185                 190         
Gln Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val 
        195                 200                 205             
Asp Lys Thr Val Glu Arg Lys 
    210                 215 

<210> 14
<211> 28
<212> PRT
<213> Artificial Sequence

<220> 
<223> G-MYC-(G4S)3 Linker

<400> 14
Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Gly Gly Gly Gly Ser 
1               5                   10                  15      
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Leu 
            20                  25              

<210> 15
<211> 255
<212> PRT
<213> Artificial Sequence

<220> 
<223> C6ML3-9 (Anti-HER2) scFv

<400> 15
Gln Val Gln Leu Leu Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
            20                  25                  30          
Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Tyr Met 
        35                  40                  45              
Gly Leu Ile Tyr Pro Gly Asp Ser Asp Thr Lys Tyr Ser Pro Ser Phe 
    50                  55                  60                  
Gln Gly Gln Val Thr Ile Ser Val Asp Lys Ser Val Ser Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Trp Ser Ser Leu Lys Pro Ser Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      
Ala Arg His Asp Val Gly Tyr Cys Ser Ser Ser Asn Cys Ala Lys Trp 
            100                 105                 110         
Pro Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 
Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
145                 150                 155                 160 
Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Asn Asn 
                165                 170                 175     
Tyr Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
            180                 185                 190         
Ile Tyr Asp His Thr Asn Arg Pro Ala Gly Val Pro Asp Arg Phe Ser 
        195                 200                 205             
Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Phe Arg 
    210                 215                 220                 
Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Trp Asp Tyr Thr Leu 
225                 230                 235                 240 
Ser Gly Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
                245                 250                 255 

<210> 16
<211> 247
<212> PRT
<213> Artificial Sequence

<220> 
<223> h528 (Anti-EGFR) scFv

<400> 16
Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      
Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Asn Ile Val His Asn 
            20                  25                  30          
Asn Gly Ile Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asp Arg Phe Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      
Ser His Ile Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         
Arg Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly 
    130                 135                 140                 
Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser 
145                 150                 155                 160 
Tyr Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp 
                165                 170                 175     
Met Gly Asn Ile Tyr Pro Gly Ser Gly Gly Thr Asn Tyr Ala Glu Lys 
            180                 185                 190         
Phe Lys Asn Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala 
        195                 200                 205             
Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr 
    210                 215                 220                 
Cys Ala Arg Ser Gly Gly Pro Tyr Phe Phe Asp Tyr Trp Gly Gln Gly 
225                 230                 235                 240 
Thr Leu Val Thr Val Ser Ser 
                245         

<210> 17
<211> 227
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized 15-2b scFv

<400> 17
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Asn Thr Ser 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Ile Ala Thr Tyr Tyr Cys Leu Gln Tyr Ile Asn Tyr Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly 
            100                 105                 110         
Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
        115                 120                 125             
Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn 
    130                 135                 140                 
Tyr Trp Met Asn Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp 
145                 150                 155                 160 
Val Gly Glu Ile Arg Ser Lys Ser Asn Asn Tyr Ala Thr His Tyr Ala 
                165                 170                 175     
Glu Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn 
            180                 185                 190         
Thr Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val 
        195                 200                 205             
Tyr Tyr Cys Thr Asn Arg Tyr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
    210                 215                 220                 
Val Ser Ser 
225         

<210> 18
<211> 219
<212> PRT
<213> Artificial Sequence

<220> 
<223> hHB12b (Anti-CD19) VH-CH1

<400> 18
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Ser 
            20                  25                  30          
Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Ser Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Ser Gly Phe Ile Thr Thr Val Leu Asp Phe Asp Tyr Trp Gly 
            100                 105                 110         
Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
        115                 120                 125             
Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
    130                 135                 140                 
Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 
Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     
Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
            180                 185                 190         
Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
        195                 200                 205             
Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val 
    210                 215                 

<210> 19
<211> 217
<212> PRT
<213> Artificial Sequence

<220> 
<223> hHB12b (Anti-CD19) VL-Ckappa

<400> 19
Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      
Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Glu Ser Val Asp Thr Phe 
            20                  25                  30          
Gly Ile Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Asp Gln Ser Pro 
        35                  40                  45              
Lys Leu Leu Ile His Ala Ala Ser Asn Gln Gly Ser Gly Val Pro Ser 
    50                  55                  60                  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn 
65                  70                  75                  80  
Ser Leu Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Lys 
                85                  90                  95      
Glu Val Pro Phe Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Thr 
            100                 105                 110         
Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
        115                 120                 125             
Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
    130                 135                 140                 
Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
145                 150                 155                 160 
Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
                165                 170                 175     
Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
            180                 185                 190         
Lys Leu Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
        195                 200                 205             
Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215         

<210> 20
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2F2 (Anti-CD20) VH-CH1

<400> 20
Met Glu Leu Gly Leu Ser Trp Ile Phe Leu Leu Ala Ile Leu Lys Gly 
1               5                   10                  15      
Val Gln Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          
Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              
Asn Asp Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  
Glu Trp Val Ser Thr Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala 
65                  70                  75                  80  
Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys 
                85                  90                  95      
Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu 
            100                 105                 110         
Tyr Tyr Cys Ala Lys Asp Ile Gln Tyr Gly Asn Tyr Tyr Tyr Gly Met 
        115                 120                 125             
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr 
    130                 135                 140                 
Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
145                 150                 155                 160 
Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
                165                 170                 175     
Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
            180                 185                 190         
Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
        195                 200                 205             
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
    210                 215                 220                 
Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val 
225                 230                 235                 

<210> 21
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2F2 (Anti-CD20) VL-Ckappa

<400> 21
Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      
Asp Thr Thr Gly Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
            20                  25                  30          
Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser 
        35                  40                  45              
Val Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
    50                  55                  60                  
Arg Leu Leu Ile Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala 
65                  70                  75                  80  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      
Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser 
            100                 105                 110         
Asn Trp Pro Ile Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Thr 
        115                 120                 125             
Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    130                 135                 140                 
Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
145                 150                 155                 160 
Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                165                 170                 175     
Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            180                 185                 190         
Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        195                 200                 205             
Lys Leu Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    210                 215                 220                 
Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230             

<210> 22
<211> 232
<212> PRT
<213> Artificial Sequence

<220> 
<223> Knob Hinge CH2-CH3

<400> 22
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr 
    130                 135                 140                 
Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 
Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         

<210> 23
<211> 207
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBU12 VL-crossover CH1

<400> 23
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      
Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          
His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              
Asp Thr Ser Lys Leu Ala Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  
Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu 
65                  70                  75                  80  
Asp Val Ala Val Tyr Tyr Cys Phe Gln Gly Ser Val Tyr Pro Phe Thr 
                85                  90                  95      
Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Ser Ala Ser Thr 
            100                 105                 110         
Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
        115                 120                 125             
Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
    130                 135                 140                 
Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
145                 150                 155                 160 
Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
                165                 170                 175     
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
            180                 185                 190         
Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val 
        195                 200                 205         

<210> 24
<211> 227
<212> PRT
<213> Artificial Sequence

<220> 
<223> hBU12 VH-crossover Ckappa

<400> 24
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Thr Ser 
            20                  25                  30          
Gly Met Gly Val Gly Trp Ile Arg Gln His Pro Gly Lys Gly Leu Glu 
        35                  40                  45              
Trp Ile Gly His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala 
    50                  55                  60                  
Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      
Cys Ala Arg Met Glu Leu Trp Ser Tyr Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Leu Val Thr Val Ser Ser Ala Ser Val Ala Ala Pro Ser Val 
        115                 120                 125             
Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser 
    130                 135                 140                 
Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln 
145                 150                 155                 160 
Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val 
                165                 170                 175     
Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu 
            180                 185                 190         
Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Leu Tyr Ala Cys Glu 
        195                 200                 205             
Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg 
    210                 215                 220                 
Gly Glu Cys 
225         

<210> 25
<211> 227
<212> PRT
<213> Artificial Sequence

<220> 
<223> hole hinge-CH2-CH3

<400> 25
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             
Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 
Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val 
            180                 185                 190         
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 
Pro Gly Lys 
225         

<210> 26
<211> 211
<212> PRT
<213> Artificial Sequence

<220> 
<223> C6ML3-9 VL-crossover CH1

<400> 26
Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      
Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Asn Asn 
            20                  25                  30          
Tyr Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              
Ile Tyr Asp His Thr Asn Arg Pro Ala Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  
Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Phe Arg 
65                  70                  75                  80  
Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Trp Asp Tyr Thr Leu 
                85                  90                  95      
Ser Gly Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ser 
            100                 105                 110         
Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             
Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 
Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 
Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     
Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         
Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             
Lys Lys Val 
    210     

<210> 27
<211> 236
<212> PRT
<213> Artificial Sequence

<220> 
<223> C6ML3-9 VH-crossover Ckappa

<400> 27
Gln Val Gln Leu Leu Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
            20                  25                  30          
Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Tyr Met 
        35                  40                  45              
Gly Leu Ile Tyr Pro Gly Asp Ser Asp Thr Lys Tyr Ser Pro Ser Phe 
    50                  55                  60                  
Gln Gly Gln Val Thr Ile Ser Val Asp Lys Ser Val Ser Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Trp Ser Ser Leu Lys Pro Ser Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      
Ala Arg His Asp Val Gly Tyr Cys Ser Ser Ser Asn Cys Ala Lys Trp 
            100                 105                 110         
Pro Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        115                 120                 125             
Ser Ala Ser Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
    130                 135                 140                 
Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
145                 150                 155                 160 
Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
                165                 170                 175     
Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
            180                 185                 190         
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
        195                 200                 205             
Glu Lys His Lys Leu Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
    210                 215                 220                 
Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235     

<210> 28
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> CH1 primer F

<400> 28
ctggtcaccg tctcctcagc ctccaccaag ggaccatcg                            39


<210> 29
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> CH1 primer R

<400> 29
gtcgactttg tcacaagatt tgggc                                          25


<210> 30
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ckappa primer F

<400> 30
accaaggtgg agatcaaacg gactgtggct gcaccatct                            39


<210> 31
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ckappa primer R

<400> 31
ctcgaggcac tctcccctgt tgaagc                                         26


<210> 32
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Fc primer F

<400> 32
ggtggacaag agagttgagc ccaaatcttg tgac                                 34


<210> 33
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Fc primer R

<400> 33
caattgtcca ctgccacccc cgcttga                                        27


<210> 34
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> hE11VL primer F

<400> 34
ggcccagccg gccgatgttg tgatgactca gtc                                  33


<210> 35
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> hE11VL- partial Ckappa primer R

<400> 35
gtgcagccac agtccgtttg atctccacct tggtc                                35


<210> 36
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3VL primer F

<400> 36
ggcccagccg gccgacatcg tgatgaccca g                                   31


<210> 37
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3VL-partial Ckappa primer R

<400> 37
gtgcagccac agtccgtttg atttccacct tggtc                                35


<210> 38
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2bVL primer F

<400> 38
ggcccagccg gccgacatcc agatgaccca g                                   31


<210> 39
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2bVL- partial Ckappa primer R

<400> 39
gtgcagccac agtccgtttg atctccagct tggtc                                35


<210> 40
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> hE11VH primer F

<400> 40
agatctcagg tgcagctggt gcag                                           24


<210> 41
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> hE11VH- partial CH1 primer R

<400> 41
tcccttggtg gaggctgagg agacggtgac caggg                                35


<210> 42
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3VLH primer F

<400> 42
agatctcagg tgcagctggt gcaatc                                         26


<210> 43
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3VH-partial CH1 primer R

<400> 43
gtcccttggt ggaggctgag gagacggtga ccag                                 34


<210> 44
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2bVH primer F

<400> 44
agatctgagg tgcagctggt ggag                                           24


<210> 45
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2bVH- partial CH1 primer R

<400> 45
gcccttggtg gaggctgagg agacggtgac caggg                                35


<210> 46
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P1

<400> 46
caattgcagg ttcagctgca agagtctggc cctgggttgg ttaagccc                  48


<210> 47
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P2

<400> 47
cagtacaagt cagactgagg gtctgggagg gcttaaccaa cccagggcc                 49


<210> 48
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P3

<400> 48
cagtctgact tgtactgtgt ctgggggttc aatcagcact tctggtatg                 49


<210> 49
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P4

<400> 49
ctgggtgctg cctaatccag cctacaccca taccagaagt gctgattg                  48


<210> 50
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P5

<400> 50
ggattaggca gcacccaggg aagtgtctgg agtggattgg acacatttgg                50


<210> 51
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P6

<400> 51
aacagtaata gacagcaaca tcctctggct ccaggctgct gattgtg                   47


<210> 52
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P7

<400> 52
caagagatat aacccagccc tgaagagcag agtgacaatc tctgtggata c              51


<210> 53
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P8

<400> 53
gacagcttga ggctaaactg gttcttggag gtatccacag agattgtcac                50


<210> 54
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P9

<400> 54
gtttagcctc aagctgtcca gtgtgacagc tgcagatact gctgtctac                 49


<210> 55
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P10

<400> 55
aaacagtaat agacagcaac atcctctggc tccaggctgc tgattgtg                  48


<210> 56
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P11

<400> 56
ggaactttgg tcctactatt ttgactactg gggccaaggc acccttg                   47


<210> 57
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P12

<400> 57
gccccctgac ccgccacctc ctgaggagac tgtgacaagg gtgccttggc ccc            53


<210> 58
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P13

<400> 58
ggtggatcgg ggggtggcgg atctgaaatt gttctcaccc agtctccagc aac            53


<210> 59
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P14

<400> 59
cagggtagcc ctttcccctg gagagagaga cagggttgct ggagactggg tg             52


<210> 60
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P15

<400> 60
ggggaaaggg ctaccctgag ctgcagtgcc agctcaagtg taagttacat gc             52


<210> 61
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P16

<400> 61
ctgggagcct gccctggctt ctgctggtac cagtgcatgt aacttacact tg             52


<210> 62
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P17

<400> 62
gccagggcag gctcccagac tcctgattta tgacacatcc aaactggctt c              51


<210> 63
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P18

<400> 63
ccagacccac tgccactgaa ccttgctgga ataccagaag ccagtttgga tg             52


<210> 64
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P19

<400> 64
cagtggcagt gggtctggaa cagattttac actcacaatc agcagcctgg                50


<210> 65
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P20

<400> 65
gaaaacagta atagacagca acatcctctg gctccaggct gctgattgtg                50


<210> 66
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P21

<400> 66
gctgtctatt actgttttca ggggagtgta tacccattca cttttggc                  48


<210> 67
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12 dsFv primer P22

<400> 67
acgcgttctt ttgatttcca actttgtccc gcagccaaaa gtgaatggg                 49


<210> 68
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P23

<400> 68
gtcgaccaat tgggaggtgg cggatcccag gtgcagctgc aggagtcggg                50


<210> 69
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P24

<400> 69
acagggtctg tgaaggcttc accagtcctg ggcccgactc ctgcagctgc                50


<210> 70
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P25

<400> 70
agccttcaca gaccctgtcc ctcacctgca ctgtctctgg tggctccatc ag             52


<210> 71
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P26

<400> 71
ggcggatcca actccagtag taatcaccac tgctgatgga gccaccagag ac             52


<210> 72
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P27

<400> 72
actggagttg gatccgccag cccccaggga agtgcctgga gtggattggg                50


<210> 73
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P28

<400> 73
ggttgtagtc ggtgctccca ctgtaataga tgtacccaat ccactccagg ca             52


<210> 74
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P29

<400> 74
tgggagcacc gactacaacc cgtccctcaa gagtcgagtc accatgtccg ta             52


<210> 75
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P30

<400> 75
accttcaggg aaaactgatt cttggacgtg tctacggaca tggtgactcg                50


<210> 76
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P31

<400> 76
tcagttttcc ctgaaggtca actctgtgac cgccgcagac acggctgtgt                50


<210> 77
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P32

<400> 77
ccccactcca aaaatcgaca ctctcgcaca gtaatacaca gccgtgtctg cgg            53


<210> 78
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P33

<400> 78
tcgatttttg gagtggggac atttgactac tggggccagg gcaccctggt                50


<210> 79
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P34

<400> 79
accgccccct gacccgccac ctccgcttga gacggtgacc agggtgccct ggcc           54


<210> 80
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P35

<400> 80
ggatcggggg gtggcggatc tgaaattgtg atgacacagt ctccagccac cctgtc         56


<210> 81
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P36

<400> 81
gcaggagagg gtggctcttt cccctggaga caaagacagg gtggctggag ac             52


<210> 82
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P37

<400> 82
agagccaccc tctcctgcag ggccagtcag agtgttagca gctactta                  48


<210> 83
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P38

<400> 83
agcctggcca ggtttctgtt ggtaccaggc taagtagctg ctaacact                  48


<210> 84
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P39

<400> 84
cagaaacctg gccaggctcc caggctcctc atctatgatg catccaacag                50


<210> 85
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P40

<400> 85
actgccactg aacctggctg ggatgccagt ggccctgttg gatgcatcat ag             52


<210> 86
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P41

<400> 86
gccaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag                50


<210> 87
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P42

<400> 87
aatacactgc aaaatcttca ggctctaggc tgctgatggt gagagtgaag                50


<210> 88
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P43

<400> 88
gaagattttg cagtgtatta ctgtcaccag tatggtagca cacctctcac tt             52


<210> 89
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> 11F8 dsFv primer P44

<400> 89
acgcgttttg atctccgcct tggtcccgca gccgaaagtg agaggtgtgc ta             52


<210> 90
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> linker-Mfel-hBU12 VH primer F

<400> 90
gtggtggttc aggacaattg ggaggtggcg gatcccaggt tcagctgcaa gag            53


<210> 91
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sall-linker-Mfel-hBU12 VH primer R

<400> 91
gtcgacctgg tcaccgtctc ctcagcctcc accggtggtg gttcaggaca at             52


<210> 92
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> hBU12VL-Mlu l-6xHis-Clal primer R

<400> 92
atcgatttaa tgatgatgat gatgatgacg cgttcttttg atttccaact ttg            53


<210> 93
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> MfeI-h11F8 VH primer F

<400> 93
caattgggag gtggcggatc ccaggtgcag ctgcaggag                            39


<210> 94
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> MluI-11F8VL primer R

<400> 94
acgcgttttg atctccgcct tggtc                                          25


<210> 95
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> MfeI-DNSVH primer F

<400> 95
caattgggag gtggcggatc cagtgaagtg aagcttgag                            39


<210> 96
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> MluI-DNSVL primer R

<400> 96
acgcgtccgt tttatttcca actt                                           24


<210> 97
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> MfeI-hCC49VH primer F

<400> 97
caattgggag gtggcggatc ccaggtgcag ctggtgcag                            39


<210> 98
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> MluI-hCC49VL primer R

<400> 98
acgcgttttg atctccacct tggtc                                          25


<210> 99
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> 15-2b- knob: VL-Ckappa primer F

<400> 99
agatctgaca tccagatgac ccag                                           24


<210> 100
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> 15-2b- knob: VL-Ckappa primer R

<400> 100
tatcgatgtt taaacctagc actctcccct gttgaa                               36


<210> 101
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> 15-2b- knob: VH-CH1 primer F

<400> 101
ggcccagccg gccgaggtgc agctggtgga g                                   31


<210> 102
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> 15-2b- knob: VH-CH1 primer R

<400> 102
aagttttttg tcgaccgtgg                                                20


<210> 103
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> 15-2b- knob: huIgG1-upper hinge primer F

<400> 103
gacaaaactc acacatgccc accgtgc                                        27


<210> 104
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> 15-2b- knob: CH1-partical hinge primer R

<400> 104
gcatgtgtga gttttgtcac aagatttggg ctcaac                               36


<210> 105
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> 15-2b- knob: CH3 primer R

<400> 105
ctcgagttta cccggagaca ggga                                           24


<210> 106
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3- knob:104 VL-Ckappa primer F

<400> 106
agatctgaca tcgtgatgac ccagtctc                                       28


<210> 107
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3- knob:104 VL-Ckappa primer R

<400> 107
tatcgatgtt taaacctagc actctcccct gttgaa                               36


<210> 108
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3- knob:104 VH-CH1 primer F

<400> 108
caggtgcagc tggtgcaat                                                 19


<210> 109
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> h6-3- knob:104 VH-CH1 primer R

<400> 109
aactctcttg tccaccttgg                                                20


<210> 110
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> BU12-hole: VH-Ckappa-partial hinge primer F

<400> 110
agccggccca ggttcagctg caagagtctg gc                                   32


<210> 111
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> BU12-hole: VH-Ckappa-partial hinge primer R

<400> 111
gcatgtgtga gttttgtcac actctcccct gttgaagct                            39


<210> 112
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> BU12-hole: VL-partial VH-CH1-upper Hinge primer F

<400> 112
gaaattgttc tcacccagtc tcc                                            23


<210> 113
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> BU12-hole: VL-partial VH-CH1-upper Hinge primer R

<400> 113
ttaacaagat ttgggctcaa c                                              21


<210> 114
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> BU12-hole: partial VL-hCH1 primer F

<400> 114
gttggaaatc aaaagatcct cagcctccac caagggccca tcg                       43


<210> 115
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: VH primer

<400> 115
ggcccagccg gcccaggtgc agctgttgca gtctggg                              37


<210> 116
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: VH-Ckappa-partial hinge primer F

<400> 116
aggtgcagct gttgcagtct ggg                                            23


<210> 117
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: VH-Ckappa-partial hinge primer R

<400> 117
gcatgtgtga gttttgtcac actctcccct gttgaagct                            39


<210> 118
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: VL-partial VH primer F

<400> 118
ctgaccgtcc taggttcctc agcctccacc aagggcccat cg                        42


<210> 119
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: VL-partial VH primer R

<400> 119
acctaggacg gtcagcttgg tcccgccgcc gaacacccag cccga                     45


<210> 120
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: VL primer F

<400> 120
ctgccagatc tcagtctgtg ttgacgcag                                      29


<210> 121
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: VL primer R

<400> 121
acctaggacg gtcagcttgg tcccgccgcc gaacacccag cccga                     45


<210> 122
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> alpha-Her2-hole: CH1-upper hinge primer R

<400> 122
ttaacaagat ttgggctcaa c                                              21


<210> 123
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b Bgl-VH-1 primer F

<400> 123
gaagatctga ggtgcagctg gtggagtctg ggggaggctt ggtccag                   47


<210> 124
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-2 primer R

<400> 124
agaggctgca caggagagtt tcagggaccc cccaggctgg accaagcctc c              51


<210> 125
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-3 primer F

<400> 125
tcctgtgcag cctctgggtt caccttcagt aactactgga tgaactgggt c              51


<210> 126
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-4 primer R

<400> 126
gccaacccac tccagccctt tcccggaagc ctggcggacc cagttcatcc a              51


<210> 127
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-5 primer F

<400> 127
ctggagtggg ttggcgaaat tagatcgaaa tctaataatt atgcgacaca t                50


<210> 128
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-6 primer R

<400> 128
ggagatggtg aacctccctt tcacagactc cgcataatgt gtcgcataat t              51


<210> 129
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-7 primer F

<400> 129
aggttcacca tctccagaga tgattcaaag aacacggcgt atctgcaaat g              51


<210> 130
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-8

<400> 130
gtaatacacg gccgtgtcct cggttttcag gctgttcatt tgcagatacg c              51


<210> 131
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-9 (T93S) primer F

<400> 131
acggccgtgt attactgttc caacagatac tactggggcc aaggaaccct g              51


<210> 132
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VH-10 primer R

<400> 132
acctttggtg gaggctgagg agacggtgac cagggttcct tggcc                     45


<210> 133
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> homo 3' IgG2 CH1-SalI primer R

<400> 133
acgcgtcgac tttgcgctca actgtctt                                       28


<210> 134
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b sfi-VL-1 primer F

<400> 134
tgctggggcc cagccggccg acatccagat gacccagtct cca                       43


<210> 135
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-2 primer R

<400> 135
ggtgactctg tctcctacag atgcagacag ggaggatgga gactgggtca t              51


<210> 136
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-3 primer F

<400> 136
ggagacagag tcaccatcac ttgcaaggcc agtcaggatg taaatacttc t              51


<210> 137
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-4 primer R

<400> 137
aggggctttc cctggtttct gctgatacca ggctacagaa gtatttacat c              51


<210> 138
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-5 primer F

<400> 138
ccagggaaag cccctaagct cctgatctac tgggcatcca cccggcacac t              51


<210> 139
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-6 primer R

<400> 139
cccagatcca cttccactga accttgatgg gaccccagtg tgccgggtgg a              51


<210> 140
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-7 primer F

<400> 140
ggaagtggat ctgggacaga ttttactttc accatcagca gcctgcagcc t              51


<210> 141
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-8 primer R

<400> 141
gatatattgc agacagtaat atgttgcaat atcttcaggc tgcaggctgc t              51


<210> 142
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-9 primer F

<400> 142
tgtctgcaat atatcaacta tccgtacacg tttggccagg ggaccaagct g              51


<210> 143
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2b VL-10 primer R

<400> 143
tggtgcagcc acagtccgtt tgatctccag cttggtcccc tg                        42


<210> 144
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> homo 3' Ckappa cys-XhoI primer R

<400> 144
ccgctcgagg cactctcccc tgttgaagct ctttgtgacg ggcgagctca ggccctg        57


<210> 145
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH01 primer F

<400> 145
caggtgcaac tggttcagag cggcgcggaa gtgaaaaagc cgggcgcgtc ggtt           54


<210> 146
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH02 primer R

<400> 146
aaaggtatag cctgaggctt tgcagctcac tttaaccgac gcgcccgg                  48


<210> 147
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH03 primer F

<400> 147
tcaggctata cctttacgag ctactggatg cattgggtgc gccaggcc                  48


<210> 148
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH04 primer R

<400> 148
aatgttaccc atccattcca ggccctgacc cggggcctgg cgcaccca                  48


<210> 149
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH05 primer F

<400> 149
tggatgggta acatttatcc gggcagcggt ggcaccaact atgcggaa                  48


<210> 150
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH06 primer R

<400> 150
atcacgcgtc atggtcacgc ggttcttaaa tttttccgca tagttggt                  48


<210> 151
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH07 primer F

<400> 151
accatgacgc gtgataccag catttcgacg gcctatatgg aactgagc                  48


<210> 152
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH08 primer R

<400> 152
gtaatacacg gcggtgtcat cgctacgcag gcggctcagt tccatata                  48


<210> 153
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH09 primer F

<400> 153
accgccgtgt attactgcgc gcgcagtggc ggtccgtatt ttttcgat                  48


<210> 154
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VH10 primer R

<400> 154
cgagctcacg gtaaccagcg taccctggcc ccagtaatcg aaaaaatacg g              51


<210> 155
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> (G4S)2-ahEGFR VH primer F

<400> 155
ggcggtggtg ggtcgggtgg cggcggatct caggtgcaac tggtt                     45


<210> 156
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> ahEGFR VH-stop-Cal primer R

<400> 156
ccatcgattt acgagctcac ggtaac                                         26


<210> 157
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL01 primer F

<400> 157
gatattgtga tgacccagag cccgctgagc ctgccggtga ccccaggc                  48


<210> 158
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL02 primer R

<400> 158
ctgcgagctg cggcagctaa tcgacgccgg ttcgcctggg gtcaccgg                  48


<210> 159
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL03 primer F

<400> 159
tgccgcagct cgcagaacat cgtgcataat aacggcatta cctatctg                  48


<210> 160
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL04 primer R

<400> 160
cgggctttgg cccggtttct gcagatacca ttccagatag gtaatgcc                  48


<210> 161
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL05 primer F

<400> 161
ccgggccaaa gcccgcagct gttaatttat aaagtgagcg atcgcttt                  48


<210> 162
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL06 primer R

<400> 162
accgctgccc gaaaagcgat ccggcacgcc gctaaagcga tcgctcac                  48


<210> 163
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL07 primer F

<400> 163
ttttcgggca gcggtagtgg caccgatttt acgctgaaaa ttagccgc                  48


<210> 164
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL08 primer R

<400> 164
gcagtaatac acgccaacat cctccgcttc cacgcggcta attttcag                  48


<210> 165
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL09 primer F

<400> 165
ggcgtgtatt actgctttca gggcagccat atcccgccaa cctttggc                  48


<210> 166
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> h528VL10 primer R

<400> 166
cgcgcgttta atttccactt tggtgccttg gccaaaggtt ggcgg                     45


<210> 167
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Mfe-ahEGFR VL primer F

<400> 167
caattggata ttgtgatgac ccag                                           24


<210> 168
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> ahEGFR VL-(G4S)2 primer R

<400> 168
cgacccacca ccgcccgagc caccgccacc cgcgcgttta atttc                     45


<210> 169
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sal-G-myc-G4S primer F

<400> 169
acgcgtcgac ggggaacaaa aactcatctc agaagaggat ctgggaggcg gtggcagt       58


<210> 170
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> G2S-G4SX2-MfeI primer F

<400> 170
ggtggcagtg gtggtggtgg atcaggaggt ggcggatccc aattgcaggt gcagctg        57


<210> 171
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Her2 scFv-stop-ClaI primer R

<400> 171
atcgattcaa cctaggacgg tcagctt                                        27


<210> 172
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> mfe1-h15-2bVL primer F

<400> 172
caattggaca tccagatgac ccagtctcca                                     30


<210> 173
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> h15-2bscFv-ClaI-Sbfl primer R

<400> 173
cccctgcagg catcgattta tgaggagacg gtgac                                35


<210> 174
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> NaeI-X+hHB12bVL-1 primer F

<400> 174
gccggccgag atcgtgctga cccagagccc cgacttccag agc                       43


<210> 175
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-2 primer R

<400> 175
ctctgcaggt gatggtcacc ttctccttgg gggtcacgct ctggaagtcg ggg            53


<210> 176
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-3 primer F

<400> 176
gtgaccatca cctgcagagc cagcgagagc gtggacacct tcggcatcag cttc           54


<210> 177
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-4 priemr R

<400> 177
gctctggtcg ggcttctgct ggaaccagtt catgaagctg atgccgaagg tg             52


<210> 178
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-5 primer F

<400> 178
gaagcccgac cagagcccca agctgctgat ccacgccgcc agcaaccagg g              51


<210> 179
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-6 primer R

<400> 179
cttccgctgc cgctgaatct gctgggcacg ccgctgccct ggttgctggc g              51


<210> 180
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-7 primer F

<400> 180
ttcagcggca gcggaagcgg caccgacttc accctgacca tcaacagcct gg             52


<210> 181
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-8 primer R

<400> 181
ctctgctggc agtagtaggt tgctgcgtcc tcggcctcca ggctgttgat ggtc           54


<210> 182
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVL-9 primer F

<400> 182
aacctactac tgccagcaga gcaaggaggt gcccttcacc ttcggcggcg gc             52


<210> 183
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> DraIII+hHB12bVL-10 primer R

<400> 183
gacactcggt gcagccacag tcttgatctc caccttggtg ccgccgccga ag             52


<210> 184
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> HpaI+hHB12bVH-1 primer F

<400> 184
gttaacgagg tgcagctggt ggagagcggc ggcggcctgg tgca                      44


<210> 185
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-2 primer R

<400> 185
cgctggcggc gcagctcagt ctcaggctgc cgccgggctg caccaggccg ccgc           54


<210> 186
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-3 primer F

<400> 186
gctgcgccgc cagcggcttc accttcagca gcagctggat gaactgggtg agac           54


<210> 187
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-4 primer R

<400> 187
gattctgccc acccactcca ggcccttgcc gggggcctgt ctcacccagt tcatcc         56


<210> 188
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-5 primrer F

<400> 188
gagtgggtgg gcagaatcta ccccggcgac ggcgacacca actacaacgg caagttc        57


<210> 189
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-6 primer R

<400> 189
tcttgctgtc gtctctgctg atggtgaatc tgcccttgaa cttgccgttg tagttg         56


<210> 190
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-7 primer F

<400> 190
ttcagcggca gcggaagcgg caccgacttc accctgacca tcaacagcct gg             52


<210> 191
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-8 primer R

<400> 191
atgaagccgc ttctggcgca gtagtacacg gcggtgtcct cggtcttcag gctgtt         56


<210> 192
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> hHB12bVH-9 primer F

<400> 192
cgccagaagc ggcttcatca ccaccgtgct ggacttcgac tactggggcc agggc          55


<210> 193
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> ApaI+hHB12bVH-10 primer R

<400> 193
gggccctttg gtggaggcgc tgctcacggt caccagggtg ccctggcccc agtag          55


<210> 194
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> NaeI-X-aCD20VL-1 primer F

<400> 194
gccggccatg gaagccccag ctcagcttct cttcctcctg ctactctggc                50


<210> 195
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-2 primer R

<400> 195
ctggagactg tgtcaacaca atttctccgg tggtatctgg gagccagagt agcaggagga     60

ag                                                                    62


<210> 196
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-3 primer F

<400> 196
aattgtgttg acacagtctc cagccaccct gtctttgtct ccaggggaaa gagccaccc      59


<210> 197
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-4 primer R

<400> 197
caggctaagt agctgctaac actctgactg gccctgcagg agagggtggc tctttcccc      59


<210> 198
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-5 primer F

<400> 198
tgttagcagc tacttagcct ggtaccaaca gaaacctggc caggctccca ggctcctc       58


<210> 199
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-6 primer R

<400> 199
ctggctggga tgccagtggc cctgttggat gcatcataga tgaggagcct gggagcc        57


<210> 200
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-7 primer F

<400> 200
actggcatcc cagccaggtt cagtggcagt gggtctggga cagacttcac tctcaccat      59


<210> 201
<211> 65
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-8 primer R

<400> 201
ctgacagtaa taaactgcaa aatcttcagg ctctaggctg ctgatggtga gagtgaagtc     60

tgtcc                                                                 65


<210> 202
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VL-9 primer F

<400> 202
gaagattttg cagtttatta ctgtcagcag cgtagcaact ggccgatcac cttcggccaa     60

gg                                                                    62


<210> 203
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> DraIII-aCD20VL-10 primer R

<400> 203
gacactcggt gcagccacag ttttaatctc cagtcgtgtc ccttggccga aggtgatc       58


<210> 204
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> HpaI+aCD20VH-1 primer F

<400> 204
gttaacatgg agttgggact gagctggatt ttccttttgg ctatttta                  48


<210> 205
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-2 primer R

<400> 205
ctccaccagc tgcacttcac actggacacc ttttaaaata gccaaaagga aaatccagc      59


<210> 206
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VhH-3 primer F

<400> 206
gaagtgcagc tggtggagtc tgggggaggc ttggtacagc ctggcaggtc cctg           54


<210> 207
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-4 primer R

<400> 207
cataatcatt aaaggtgaat ccagaggctg cacaggagag tctcagggac ctgccagg       58


<210> 208
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-5 primer F

<400> 208
gcctctggat tcacctttaa tgattatgcc atgcactggg tccggcaagc tccagggaag     60


<210> 209
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-6 primer R

<400> 209
ggaaccacta ttccaactaa tagttgagac ccactccagg cccttccctg gagcttgcc      59


<210> 210
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-7 primer F

<400> 210
tcaactatta gttggaatag tggttccata ggctatgcgg actctgtgaa gggccgattc     60


<210> 211
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-8 primer R

<400> 211
gatacaggga cttcttggcg ttgtctctgg agatggtgaa tcggcccttc acagag         56


<210> 212
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-9 primer F

<400> 212
cgccaagaag tccctgtatc tgcaaatgaa cagtctgaga gctgaggaca cggcc          55


<210> 213
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-10 primer R

<400> 213
gtagtagttg ccgtactgta tatcttttgc acagtaatac aaggccgtgt cctcagc        57


<210> 214
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> aCD20VH-11 primer F

<400> 214
agatatacag tacggcaact actactacgg tatggacgtc tggggccaag ggaccac        57


<210> 215
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> ApaI-aCD20VH-12 primer R

<400> 215
gggccctttg gtggaggctg aggagacggt gaccgtggtc ccttggccc                 49


