                               SEQUENCE LISTING

<110> GENENTECH, INC. ET AL.
 
<120> METHODS AND COMPOSITIONS FOR SECRETION OF HETEROLOGOUS 
      POLYPEPTIDES

<130> P5804R1-WO

<140>
<141>

<150> 62/108,476
<151> 2015-01-27

<150> 62/107,981
<151> 2015-01-26

<150> 61/953,629
<151> 2014-03-14

<160> 62    

<170> PatentIn version 3.5

<210> 1
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 1
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 2
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 2
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 3
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 3
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 4
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 4
Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      


Pro Val Thr Lys Ala 
            20      


<210> 5
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 5
Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      


Pro Val Thr Lys Ala 
            20      


<210> 6
<211> 26
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 6
Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210> 7
<211> 26
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 7
Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210> 8
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 8
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Leu Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 9
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 9
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Leu Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 10
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 10
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Leu Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 11
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 11
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ile Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 12
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 12
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Ser Ala 
            20              


<210> 13
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 13
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Ile Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 14
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 14
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Ser Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 15
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 15
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Tyr Ala 
            


<210> 16
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 16
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcat ctatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 17
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 17
acgcgtatta tgaagaaaaa catcgctttt cttcttgcat ctatgttcgt tttttctatt       60

gctacaaacg cttac                                                        75


<210> 18
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 18
gcgcgcatta tgaaaaaaat ttggctcgcc ctggctggtt tagttttagc gtttagcgca       60

tcggcg                                                                  66


<210> 19
<211> 72
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 19
gcgcgcatta tgaaacaatc cacgattgcc ctggcactct taccgttact gtttacccct       60

gtgacaaaag cc                                                           72


<210> 20
<211> 72
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 20
acgcgtatta tgaaacagtc tactatcgct ctggcactct taccgttact gtttacccct       60

gtgacaaaag cc                                                           72


<210> 21
<211> 87
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 21
gcgcgcatta tgaaaattaa gactggagca cgcatcctcg cattatccgc attaacgacg       60

atgatgtttt ccgcctcggc tctcgcc                                           87


<210> 22
<211> 87
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 22
acgcgtatta tgaagatcaa gacaggcgcg cgcatcctcg cattatccgc attaacgacg       60

atgatgtttt ccgcctcggc tctcgcc                                           87


<210> 23
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 23
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcat taatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 24
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 24
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcat tgatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 25
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 25
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcac tcatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 26
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 26
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcaa ttatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 27
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 27
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcat ctatgttcgt tttttctatt       60

gctacaaacg cttcggct                                                     78


<210> 28
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 28
gcgcgcatta tgaaaaaaat ttggctcgcc ctggctggta ttgttttagc gtttagcgca       60

tcggcg                                                                  66


<210> 29
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 29
gcgcgcatta tgaaaaaaat ttggctcgcc ctggctggtt ctgttttagc gtttagcgca       60

tcggcg                                                                  66


<210> 30
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 30
gcgcgcatta tgaaaaaaat ttggctcgcc ctggctggtt tagttttagc gtttagcgca       60

tacgcg                                                                  66


<210> 31
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 31
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ala Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 32
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 32
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Gln Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 33
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 33
Met Lys Lys Asn Ile Leu Phe Leu Leu Leu Leu Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 34
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 34
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Ala Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 35
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 35
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Gln Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 36
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 36
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcag caatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 37
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 37
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcac aaatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 38
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 38
gcgcgcatta tgaagaaaaa catcctcttt cttcttctac taatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 39
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 39
gcgcgcatta tgaaaaaaat ttggctcgcc ctggctggtg ctgttttagc gtttagcgca       60

tcggcg                                                                  66


<210> 40
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 40
gcgcgcatta tgaaaaaaat ttggctcgcc ctggctggtc aggttttagc gtttagcgca       60

tcggcg                                                                  66


<210> 41
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 41
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Tyr Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 42
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 42
Met Met Thr Lys Ile Lys Leu Leu Met Leu Ile Ile Phe Tyr Leu Ile 
1               5                   10                  15      


Ile Ser Ala Ser Ala His Ala 
            20              


<210> 43
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 43
Met Met Thr Lys Ile Lys Leu Leu Met Leu Ile Ile Phe Tyr Leu Ile 
1               5                   10                  15      


Ile Ser Ala Ser Ala His Ala 
            20              


<210> 44
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 44
gcgcgcatta tgaagaaaaa catcgctttt cttcttgcat acatgttcgt tttttctatt       60

gctacaaacg cttacgct                                                     78


<210> 45
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 45
tctagaatta tgatgactaa aatcaagctt ctaatgctca ttatatttta tttaatcatt       60

tcggccagcg cccatgct                                                     78


<210> 46
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 46
tctagaatta tgatgacgaa aatcaagcta ctgatgctca ttatatttta tttaatcatt       60

tcggccagcg cccatgct                                                     78


<210> 47
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 47
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 48
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 48
Asp Ile Cys Leu Pro Arg Trp Gly Cys Leu Trp 
1               5                   10      


<210> 49
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 49
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Ile Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 50
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 50
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Ser Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 51
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 51
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 52
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 52
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Leu Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 53
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 53
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ile Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 54
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 54
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 55
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 55
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Ala Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 56
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 56
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Gln Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 57
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 57
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 58
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 58
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ala Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 59
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 59
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Gln Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 60
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 60
Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Tyr Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 61
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 61
Met Lys Lys Asn Ile Leu Phe Leu Leu Leu Leu Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210> 62
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 62
Met Met Thr Lys Ile Lys Leu Leu Met Leu Ile Ile Phe Tyr Leu Ile 
1               5                   10                  15      


Ile Ser Ala Ser Ala His Ala 
            20              


