                         SEQUENCE LISTING

<110>  TIAN, Tian
 
<120>  NOVEL RECOMBINANT BI-FUNCTIONAL FUSION PROTEINS, PREPARATION AND 
       USE THEREOF

<130>  154040-00001

<150>  US61/969,815
<151>  2014-03-24

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 1

<400>  1
cggaattcga gcccaaatct tgtg                                              24


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2

<400>  2
catgctcgag tttacccgga gacagggag                                         29


<210>  3
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3

<400>  3
cccaagcttg gggccaccat ggagcccgcc ggcccggccc                             40


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 4

<400>  4
cggaattcgt gctgaggtgt ggccctcgcc                                        30


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5

<400>  5
catgctcgag aatagtgata caggtagacc tttc                                   34


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 6

<400>  6
gcatctagag ggttagattg tattggtttg tcgatg                                 36


<210>  7
<211>  1506
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SIRPaD1-Fc-VEGFR1D2

<400>  7
atggagcccg ccggcccggc ccccggccgc ctcgggccgc tgctctgcct gctgctcgcc       60

gcgtcctgcg cctggtcagg agtggcgggt gaggaggagc tgcaggtgat tcagcctgac      120

aagtccgtat cagttgcagc tggagagtcg gccattctgc actgcactgt gacctccctg      180

atccctgtgg ggcccatcca gtggttcaga ggagctggac cagcccggga attaatctac      240

aatcaaaaag aaggccactt cccccgggta acaactgttt cagagtccac aaagagagaa      300

aacatggact tttccatcag catcagtaac atcaccccag cagatgccgg cacctactac      360

tgtgtgaagt tccggaaagg gagccctgac acggagttta agtctggagc aggcactgag      420

ctgtctgtgc gtgccaaacc ctctgccccc gtggtatcgg gccctgcggc gagggccaca      480

cctcagcacg aattcgagcc caaatcttgt gacaaaactc acacatgccc accgtgccca      540

gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc      600

ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac      660

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag      720

ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac      780

caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc      840

cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc      900

ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa      960

ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac     1020

tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc     1080

accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag     1140

gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa actcgagatt     1200

agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat tatacacatg     1260

actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat cactgttact     1320

ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat ctgggacagt     1380

agaaagggct tcatcatatc aaatgcaacg tacaaagaaa tagggcttct gacctgtgaa     1440

gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca aaccaataca     1500

atctaa                                                                1506


<210>  8
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIRPaD1-Fc-VEGFR1D2

<400>  8

Met Glu Pro Ala Gly Pro Ala Pro Gly Arg Leu Gly Pro Leu Leu Cys 
1               5                   10                  15      


Leu Leu Leu Ala Ala Ser Cys Ala Trp Ser Gly Val Ala Gly Glu Glu 
            20                  25                  30          


Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Ser Val Ala Ala Gly 
        35                  40                  45              


Glu Ser Ala Ile Leu His Cys Thr Val Thr Ser Leu Ile Pro Val Gly 
    50                  55                  60                  


Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Ala Arg Glu Leu Ile Tyr 
65                  70                  75                  80  


Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser Glu Ser 
                85                  90                  95      


Thr Lys Arg Glu Asn Met Asp Phe Ser Ile Ser Ile Ser Asn Ile Thr 
            100                 105                 110         


Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys Gly Ser 
        115                 120                 125             


Pro Asp Thr Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu Ser Val Arg 
    130                 135                 140                 


Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala Arg Ala Thr 
145                 150                 155                 160 


Pro Gln His Glu Phe Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                165                 170                 175     


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
            180                 185                 190         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
        195                 200                 205             


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
    210                 215                 220                 


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
225                 230                 235                 240 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
                245                 250                 255     


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
            260                 265                 270         


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
        275                 280                 285             


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
    290                 295                 300                 


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
305                 310                 315                 320 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
                325                 330                 335     


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
            340                 345                 350         


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
        355                 360                 365             


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
    370                 375                 380                 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Leu Glu Ile 
385                 390                 395                 400 


Ser Asp Thr Gly Arg Pro Phe Val Glu Met Tyr Ser Glu Ile Pro Glu 
                405                 410                 415     


Ile Ile His Met Thr Glu Gly Arg Glu Leu Val Ile Pro Cys Arg Val 
            420                 425                 430         


Thr Ser Pro Asn Ile Thr Val Thr Leu Lys Lys Phe Pro Leu Asp Thr 
        435                 440                 445             


Leu Ile Pro Asp Gly Lys Arg Ile Ile Trp Asp Ser Arg Lys Gly Phe 
    450                 455                 460                 


Ile Ile Ser Asn Ala Thr Tyr Lys Glu Ile Gly Leu Leu Thr Cys Glu 
465                 470                 475                 480 


Ala Thr Val Asn Gly His Leu Tyr Lys Thr Asn Tyr Leu Thr His Arg 
                485                 490                 495     


Gln Thr Asn Thr Ile 
            500     


