                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals sa
 
<120>  NOVEL METHODS FOR INDUCING AN IMMUNE RESPONSE

<130>  VB65685 PCT

<150>  GB1405921.6
<151>  2014-04-02

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RTS,S

<400>  1

Met Met Ala Pro Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
1               5                   10                  15      


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
            20                  25                  30          


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        35                  40                  45              


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    50                  55                  60                  


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Lys 
65                  70                  75                  80  


Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp Pro Asn 
                85                  90                  95      


Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys Asn Asn 
            100                 105                 110         


Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu Asn Lys 
        115                 120                 125             


Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys 
    130                 135                 140                 


Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn Lys Pro 
145                 150                 155                 160 


Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile Cys Lys 
                165                 170                 175     


Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn Ser Ser Ile Gly 
            180                 185                 190         


Leu Gly Pro Val Thr Asn Met Glu Asn Ile Thr Ser Gly Phe Leu Gly 
        195                 200                 205             


Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu 
    210                 215                 220                 


Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu 
225                 230                 235                 240 


Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser 
                245                 250                 255     


Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro Gly Tyr Arg Trp 
            260                 265                 270         


Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys 
        275                 280                 285             


Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly Met Leu Pro Val 
    290                 295                 300                 


Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr Gly Pro Cys Lys 
305                 310                 315                 320 


Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe Pro Ser Cys Cys 
                325                 330                 335     


Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser 
            340                 345                 350         


Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala Ser Val Arg Phe 
        355                 360                 365             


Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp Phe Val Gly Leu 
    370                 375                 380                 


Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met Trp Tyr Trp Gly 
385                 390                 395                 400 


Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro Leu Leu Pro Ile 
                405                 410                 415     


Phe Phe Cys Leu Trp Val Tyr Ile 
            420                 


<210>  2
<211>  546
<212>  PRT
<213>  Varicella zoster

<400>  2

Met Gly Thr Val Asn Lys Pro Val Val Gly Val Leu Met Gly Phe Gly 
1               5                   10                  15      


Ile Ile Thr Gly Thr Leu Arg Ile Thr Asn Pro Val Arg Ala Ser Val 
            20                  25                  30          


Leu Arg Tyr Asp Asp Phe His Ile Asp Glu Asp Lys Leu Asp Thr Asn 
        35                  40                  45              


Ser Val Tyr Glu Pro Tyr Tyr His Ser Asp His Ala Glu Ser Ser Trp 
    50                  55                  60                  


Val Asn Arg Gly Glu Ser Ser Arg Lys Ala Tyr Asp His Asn Ser Pro 
65                  70                  75                  80  


Tyr Ile Trp Pro Arg Asn Asp Tyr Asp Gly Phe Leu Glu Asn Ala His 
                85                  90                  95      


Glu His His Gly Val Tyr Asn Gln Gly Arg Gly Ile Asp Ser Gly Glu 
            100                 105                 110         


Arg Leu Met Gln Pro Thr Gln Met Ser Ala Gln Glu Asp Leu Gly Asp 
        115                 120                 125             


Asp Thr Gly Ile His Val Ile Pro Thr Leu Asn Gly Asp Asp Arg His 
    130                 135                 140                 


Lys Ile Val Asn Val Asp Gln Arg Gln Tyr Gly Asp Val Phe Lys Gly 
145                 150                 155                 160 


Asp Leu Asn Pro Lys Pro Gln Gly Gln Arg Leu Ile Glu Val Ser Val 
                165                 170                 175     


Glu Glu Asn His Pro Phe Thr Leu Arg Ala Pro Ile Gln Arg Ile Tyr 
            180                 185                 190         


Gly Val Arg Tyr Thr Glu Thr Trp Ser Phe Leu Pro Ser Leu Thr Cys 
        195                 200                 205             


Thr Gly Asp Ala Ala Pro Ala Ile Gln His Ile Cys Leu Lys His Thr 
    210                 215                 220                 


Thr Cys Phe Gln Asp Val Val Val Asp Val Asp Cys Ala Glu Asn Thr 
225                 230                 235                 240 


Lys Glu Asp Gln Leu Ala Glu Ile Ser Tyr Arg Phe Gln Gly Lys Lys 
                245                 250                 255     


Glu Ala Asp Gln Pro Trp Ile Val Val Asn Thr Ser Thr Leu Phe Asp 
            260                 265                 270         


Glu Leu Glu Leu Asp Pro Pro Glu Ile Glu Pro Gly Val Leu Lys Val 
        275                 280                 285             


Leu Arg Thr Glu Lys Gln Tyr Leu Gly Val Tyr Ile Trp Asn Met Arg 
    290                 295                 300                 


Gly Ser Asp Gly Thr Ser Thr Tyr Ala Thr Phe Leu Val Thr Trp Lys 
305                 310                 315                 320 


Gly Asp Glu Lys Thr Arg Asn Pro Thr Pro Ala Val Thr Pro Gln Pro 
                325                 330                 335     


Arg Gly Ala Glu Phe His Met Trp Asn Tyr His Ser His Val Phe Ser 
            340                 345                 350         


Val Gly Asp Thr Phe Ser Leu Ala Met His Leu Gln Tyr Lys Ile His 
        355                 360                 365             


Glu Ala Pro Phe Asp Leu Leu Leu Glu Trp Leu Tyr Val Pro Ile Asp 
    370                 375                 380                 


Pro Thr Cys Gln Pro Met Arg Leu Tyr Ser Thr Cys Leu Tyr His Pro 
385                 390                 395                 400 


Asn Ala Pro Gln Cys Leu Ser His Met Asn Ser Gly Cys Thr Phe Thr 
                405                 410                 415     


Ser Pro His Leu Ala Gln Arg Val Ala Ser Thr Val Tyr Gln Asn Cys 
            420                 425                 430         


Glu His Ala Asp Asn Tyr Thr Ala Tyr Cys Leu Gly Ile Ser His Met 
        435                 440                 445             


Glu Pro Ser Phe Gly Leu Ile Leu His Asp Gly Gly Thr Thr Leu Lys 
    450                 455                 460                 


Phe Val Asp Thr Pro Glu Ser Leu Ser Gly Leu Tyr Val Phe Val Val 
465                 470                 475                 480 


Tyr Phe Asn Gly His Val Glu Ala Val Ala Tyr Thr Val Val Ser Thr 
                485                 490                 495     


Val Asp His Phe Val Asn Ala Ile Glu Glu Arg Gly Phe Pro Pro Thr 
            500                 505                 510         


Ala Gly Gln Pro Pro Ala Thr Thr Lys Pro Lys Glu Ile Thr Pro Val 
        515                 520                 525             


Asn Pro Gly Thr Ser Pro Leu Ile Arg Tyr Ala Ala Trp Thr Gly Gly 
    530                 535                 540                 


Leu Ala 
545     


<210>  3
<211>  723
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M72 fusion protein

<400>  3

Met Thr Ala Ala Ser Asp Asn Phe Gln Leu Ser Gln Gly Gly Gln Gly 
1               5                   10                  15      


Phe Ala Ile Pro Ile Gly Gln Ala Met Ala Ile Ala Gly Gln Ile Arg 
            20                  25                  30          


Ser Gly Gly Gly Ser Pro Thr Val His Ile Gly Pro Thr Ala Phe Leu 
        35                  40                  45              


Gly Leu Gly Val Val Asp Asn Asn Gly Asn Gly Ala Arg Val Gln Arg 
    50                  55                  60                  


Val Val Gly Ser Ala Pro Ala Ala Ser Leu Gly Ile Ser Thr Gly Asp 
65                  70                  75                  80  


Val Ile Thr Ala Val Asp Gly Ala Pro Ile Asn Ser Ala Thr Ala Met 
                85                  90                  95      


Ala Asp Ala Leu Asn Gly His His Pro Gly Asp Val Ile Ser Val Thr 
            100                 105                 110         


Trp Gln Thr Lys Ser Gly Gly Thr Arg Thr Gly Asn Val Thr Leu Ala 
        115                 120                 125             


Glu Gly Pro Pro Ala Glu Phe Met Val Asp Phe Gly Ala Leu Pro Pro 
    130                 135                 140                 


Glu Ile Asn Ser Ala Arg Met Tyr Ala Gly Pro Gly Ser Ala Ser Leu 
145                 150                 155                 160 


Val Ala Ala Ala Gln Met Trp Asp Ser Val Ala Ser Asp Leu Phe Ser 
                165                 170                 175     


Ala Ala Ser Ala Phe Gln Ser Val Val Trp Gly Leu Thr Val Gly Ser 
            180                 185                 190         


Trp Ile Gly Ser Ser Ala Gly Leu Met Val Ala Ala Ala Ser Pro Tyr 
        195                 200                 205             


Val Ala Trp Met Ser Val Thr Ala Gly Gln Ala Glu Leu Thr Ala Ala 
    210                 215                 220                 


Gln Val Arg Val Ala Ala Ala Ala Tyr Glu Thr Ala Tyr Gly Leu Thr 
225                 230                 235                 240 


Val Pro Pro Pro Val Ile Ala Glu Asn Arg Ala Glu Leu Met Ile Leu 
                245                 250                 255     


Ile Ala Thr Asn Leu Leu Gly Gln Asn Thr Pro Ala Ile Ala Val Asn 
            260                 265                 270         


Glu Ala Glu Tyr Gly Glu Met Trp Ala Gln Asp Ala Ala Ala Met Phe 
        275                 280                 285             


Gly Tyr Ala Ala Ala Thr Ala Thr Ala Thr Ala Thr Leu Leu Pro Phe 
    290                 295                 300                 


Glu Glu Ala Pro Glu Met Thr Ser Ala Gly Gly Leu Leu Glu Gln Ala 
305                 310                 315                 320 


Ala Ala Val Glu Glu Ala Ser Asp Thr Ala Ala Ala Asn Gln Leu Met 
                325                 330                 335     


Asn Asn Val Pro Gln Ala Leu Gln Gln Leu Ala Gln Pro Thr Gln Gly 
            340                 345                 350         


Thr Thr Pro Ser Ser Lys Leu Gly Gly Leu Trp Lys Thr Val Ser Pro 
        355                 360                 365             


His Arg Ser Pro Ile Ser Asn Met Val Ser Met Ala Asn Asn His Met 
    370                 375                 380                 


Ser Met Thr Asn Ser Gly Val Ser Met Thr Asn Thr Leu Ser Ser Met 
385                 390                 395                 400 


Leu Lys Gly Phe Ala Pro Ala Ala Ala Ala Gln Ala Val Gln Thr Ala 
                405                 410                 415     


Ala Gln Asn Gly Val Arg Ala Met Ser Ser Leu Gly Ser Ser Leu Gly 
            420                 425                 430         


Ser Ser Gly Leu Gly Gly Gly Val Ala Ala Asn Leu Gly Arg Ala Ala 
        435                 440                 445             


Ser Val Gly Ser Leu Ser Val Pro Gln Ala Trp Ala Ala Ala Asn Gln 
    450                 455                 460                 


Ala Val Thr Pro Ala Ala Arg Ala Leu Pro Leu Thr Ser Leu Thr Ser 
465                 470                 475                 480 


Ala Ala Glu Arg Gly Pro Gly Gln Met Leu Gly Gly Leu Pro Val Gly 
                485                 490                 495     


Gln Met Gly Ala Arg Ala Gly Gly Gly Leu Ser Gly Val Leu Arg Val 
            500                 505                 510         


Pro Pro Arg Pro Tyr Val Met Pro His Ser Pro Ala Ala Gly Asp Ile 
        515                 520                 525             


Ala Pro Pro Ala Leu Ser Gln Asp Arg Phe Ala Asp Phe Pro Ala Leu 
    530                 535                 540                 


Pro Leu Asp Pro Ser Ala Met Val Ala Gln Val Gly Pro Gln Val Val 
545                 550                 555                 560 


Asn Ile Asn Thr Lys Leu Gly Tyr Asn Asn Ala Val Gly Ala Gly Thr 
                565                 570                 575     


Gly Ile Val Ile Asp Pro Asn Gly Val Val Leu Thr Asn Asn His Val 
            580                 585                 590         


Ile Ala Gly Ala Thr Asp Ile Asn Ala Phe Ser Val Gly Ser Gly Gln 
        595                 600                 605             


Thr Tyr Gly Val Asp Val Val Gly Tyr Asp Arg Thr Gln Asp Val Ala 
    610                 615                 620                 


Val Leu Gln Leu Arg Gly Ala Gly Gly Leu Pro Ser Ala Ala Ile Gly 
625                 630                 635                 640 


Gly Gly Val Ala Val Gly Glu Pro Val Val Ala Met Gly Asn Ser Gly 
                645                 650                 655     


Gly Gln Gly Gly Thr Pro Arg Ala Val Pro Gly Arg Val Val Ala Leu 
            660                 665                 670         


Gly Gln Thr Val Gln Ala Ser Asp Ser Leu Thr Gly Ala Glu Glu Thr 
        675                 680                 685             


Leu Asn Gly Leu Ile Gln Phe Asp Ala Ala Ile Gln Pro Gly Asp Ala 
    690                 695                 700                 


Gly Gly Pro Val Val Asn Gly Leu Gly Gln Val Val Gly Met Asn Thr 
705                 710                 715                 720 


Ala Ala Ser 
            


<210>  4
<211>  725
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M72 fusion protein (2-his)

<400>  4

Met His His Thr Ala Ala Ser Asp Asn Phe Gln Leu Ser Gln Gly Gly 
1               5                   10                  15      


Gln Gly Phe Ala Ile Pro Ile Gly Gln Ala Met Ala Ile Ala Gly Gln 
            20                  25                  30          


Ile Arg Ser Gly Gly Gly Ser Pro Thr Val His Ile Gly Pro Thr Ala 
        35                  40                  45              


Phe Leu Gly Leu Gly Val Val Asp Asn Asn Gly Asn Gly Ala Arg Val 
    50                  55                  60                  


Gln Arg Val Val Gly Ser Ala Pro Ala Ala Ser Leu Gly Ile Ser Thr 
65                  70                  75                  80  


Gly Asp Val Ile Thr Ala Val Asp Gly Ala Pro Ile Asn Ser Ala Thr 
                85                  90                  95      


Ala Met Ala Asp Ala Leu Asn Gly His His Pro Gly Asp Val Ile Ser 
            100                 105                 110         


Val Thr Trp Gln Thr Lys Ser Gly Gly Thr Arg Thr Gly Asn Val Thr 
        115                 120                 125             


Leu Ala Glu Gly Pro Pro Ala Glu Phe Met Val Asp Phe Gly Ala Leu 
    130                 135                 140                 


Pro Pro Glu Ile Asn Ser Ala Arg Met Tyr Ala Gly Pro Gly Ser Ala 
145                 150                 155                 160 


Ser Leu Val Ala Ala Ala Gln Met Trp Asp Ser Val Ala Ser Asp Leu 
                165                 170                 175     


Phe Ser Ala Ala Ser Ala Phe Gln Ser Val Val Trp Gly Leu Thr Val 
            180                 185                 190         


Gly Ser Trp Ile Gly Ser Ser Ala Gly Leu Met Val Ala Ala Ala Ser 
        195                 200                 205             


Pro Tyr Val Ala Trp Met Ser Val Thr Ala Gly Gln Ala Glu Leu Thr 
    210                 215                 220                 


Ala Ala Gln Val Arg Val Ala Ala Ala Ala Tyr Glu Thr Ala Tyr Gly 
225                 230                 235                 240 


Leu Thr Val Pro Pro Pro Val Ile Ala Glu Asn Arg Ala Glu Leu Met 
                245                 250                 255     


Ile Leu Ile Ala Thr Asn Leu Leu Gly Gln Asn Thr Pro Ala Ile Ala 
            260                 265                 270         


Val Asn Glu Ala Glu Tyr Gly Glu Met Trp Ala Gln Asp Ala Ala Ala 
        275                 280                 285             


Met Phe Gly Tyr Ala Ala Ala Thr Ala Thr Ala Thr Ala Thr Leu Leu 
    290                 295                 300                 


Pro Phe Glu Glu Ala Pro Glu Met Thr Ser Ala Gly Gly Leu Leu Glu 
305                 310                 315                 320 


Gln Ala Ala Ala Val Glu Glu Ala Ser Asp Thr Ala Ala Ala Asn Gln 
                325                 330                 335     


Leu Met Asn Asn Val Pro Gln Ala Leu Gln Gln Leu Ala Gln Pro Thr 
            340                 345                 350         


Gln Gly Thr Thr Pro Ser Ser Lys Leu Gly Gly Leu Trp Lys Thr Val 
        355                 360                 365             


Ser Pro His Arg Ser Pro Ile Ser Asn Met Val Ser Met Ala Asn Asn 
    370                 375                 380                 


His Met Ser Met Thr Asn Ser Gly Val Ser Met Thr Asn Thr Leu Ser 
385                 390                 395                 400 


Ser Met Leu Lys Gly Phe Ala Pro Ala Ala Ala Ala Gln Ala Val Gln 
                405                 410                 415     


Thr Ala Ala Gln Asn Gly Val Arg Ala Met Ser Ser Leu Gly Ser Ser 
            420                 425                 430         


Leu Gly Ser Ser Gly Leu Gly Gly Gly Val Ala Ala Asn Leu Gly Arg 
        435                 440                 445             


Ala Ala Ser Val Gly Ser Leu Ser Val Pro Gln Ala Trp Ala Ala Ala 
    450                 455                 460                 


Asn Gln Ala Val Thr Pro Ala Ala Arg Ala Leu Pro Leu Thr Ser Leu 
465                 470                 475                 480 


Thr Ser Ala Ala Glu Arg Gly Pro Gly Gln Met Leu Gly Gly Leu Pro 
                485                 490                 495     


Val Gly Gln Met Gly Ala Arg Ala Gly Gly Gly Leu Ser Gly Val Leu 
            500                 505                 510         


Arg Val Pro Pro Arg Pro Tyr Val Met Pro His Ser Pro Ala Ala Gly 
        515                 520                 525             


Asp Ile Ala Pro Pro Ala Leu Ser Gln Asp Arg Phe Ala Asp Phe Pro 
    530                 535                 540                 


Ala Leu Pro Leu Asp Pro Ser Ala Met Val Ala Gln Val Gly Pro Gln 
545                 550                 555                 560 


Val Val Asn Ile Asn Thr Lys Leu Gly Tyr Asn Asn Ala Val Gly Ala 
                565                 570                 575     


Gly Thr Gly Ile Val Ile Asp Pro Asn Gly Val Val Leu Thr Asn Asn 
            580                 585                 590         


His Val Ile Ala Gly Ala Thr Asp Ile Asn Ala Phe Ser Val Gly Ser 
        595                 600                 605             


Gly Gln Thr Tyr Gly Val Asp Val Val Gly Tyr Asp Arg Thr Gln Asp 
    610                 615                 620                 


Val Ala Val Leu Gln Leu Arg Gly Ala Gly Gly Leu Pro Ser Ala Ala 
625                 630                 635                 640 


Ile Gly Gly Gly Val Ala Val Gly Glu Pro Val Val Ala Met Gly Asn 
                645                 650                 655     


Ser Gly Gly Gln Gly Gly Thr Pro Arg Ala Val Pro Gly Arg Val Val 
            660                 665                 670         


Ala Leu Gly Gln Thr Val Gln Ala Ser Asp Ser Leu Thr Gly Ala Glu 
        675                 680                 685             


Glu Thr Leu Asn Gly Leu Ile Gln Phe Asp Ala Ala Ile Gln Pro Gly 
    690                 695                 700                 


Asp Ala Gly Gly Pro Val Val Asn Gly Leu Gly Gln Val Val Gly Met 
705                 710                 715                 720 


Asn Thr Ala Ala Ser 
                725 


