                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Detergent composition

<130>  12785-WO-PCT

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  910
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  exon
<222>  (1)..(242)

<220>
<221>  Intron
<222>  (243)..(308)

<220>
<221>  exon
<222>  (309)..(494)

<220>
<221>  Intron
<222>  (495)..(555)

<220>
<221>  exon
<222>  (556)..(714)

<220>
<221>  Intron
<222>  (715)..(765)

<220>
<221>  exon
<222>  (766)..(907)

<220>
<221>  Intron
<222>  (908)..(910)

<400>  1
atg cag ctt act aag tcc ctc ctg gta ttc gcg ctt tac atg ttt ggc         48
Met Gln Leu Thr Lys Ser Leu Leu Val Phe Ala Leu Tyr Met Phe Gly           
1               5                   10                  15                

act cag cac gtt cta gct gtg cct gtc aat ccc gag cct gat gct acg         96
Thr Gln His Val Leu Ala Val Pro Val Asn Pro Glu Pro Asp Ala Thr           
            20                  25                  30                    

agc gtc gaa aat gtt gcc ctt aaa aca ggc agc ggt gat agc cag agc        144
Ser Val Glu Asn Val Ala Leu Lys Thr Gly Ser Gly Asp Ser Gln Ser           
        35                  40                  45                        

gat ccc atc aag gcg gac ttg gag gtc aaa ggc caa agt gct ttg cct        192
Asp Pro Ile Lys Ala Asp Leu Glu Val Lys Gly Gln Ser Ala Leu Pro           
    50                  55                  60                            

ttc gac gtc gac tgc tgg gct atc ctg tgc aag ggc gcc ccg aat gtc        240
Phe Asp Val Asp Cys Trp Ala Ile Leu Cys Lys Gly Ala Pro Asn Val           
65                  70                  75                  80            

ct  gtatgtcttc ctttattgaa gctcttgatg tggcttgtat gtttgactaa             292
Leu                                                                       
                                                                          

tatatcgcac ccttag g cag cgc gtg aat gaa aag acg aaa aat agt aat        342
                    Gln Arg Val Asn Glu Lys Thr Lys Asn Ser Asn           
                                85                  90                    

cgc gat cgg agc ggt gcg aac aaa ggg cct ttc aaa gat cct cag aaa        390
Arg Asp Arg Ser Gly Ala Asn Lys Gly Pro Phe Lys Asp Pro Gln Lys           
        95                  100                 105                       

tgg ggc atc aaa gcc ctt cca cct aag aat cca tcc tgg agc gca caa        438
Trp Gly Ile Lys Ala Leu Pro Pro Lys Asn Pro Ser Trp Ser Ala Gln           
    110                 115                 120                           

gac ttc aaa tca ccc gaa gaa tac gca ttt gcg tct tcc ctt caa ggc        486
Asp Phe Lys Ser Pro Glu Glu Tyr Ala Phe Ala Ser Ser Leu Gln Gly           
125                 130                 135                 140           

gga acc aa  gtatgctaag atcatcactg cttcaatcaa tgtgttgtta                534
Gly Thr Asn                                                               
                                                                          

gctgactccg atgtgaccaa g t gcc atc cta gcg ccc gtc aac ctc gct tct      586
                          Ala Ile Leu Ala Pro Val Asn Leu Ala Ser         
                              145                 150                     

cag aac tcc caa ggc ggc gtc ttg aac ggt ttc tac tcg gcg aac aaa        634
Gln Asn Ser Gln Gly Gly Val Leu Asn Gly Phe Tyr Ser Ala Asn Lys           
    155                 160                 165                           

gta gca caa ttt gat cct agc aag ccc caa cag aca aag gga aca tgg        682
Val Ala Gln Phe Asp Pro Ser Lys Pro Gln Gln Thr Lys Gly Thr Trp           
170                 175                 180                 185           

ttt cag atc act aag ttc aca ggt gca gct gg  gtaagaactt ccagtaccat      734
Phe Gln Ile Thr Lys Phe Thr Gly Ala Ala Gly                               
                190                 195                                   

ggtcatatgc aatttactaa gaaaatacta g t cct tac tgc aag gct ctg ggg       787
                                     Pro Tyr Cys Lys Ala Leu Gly          
                                                 200                      

agt aat gat aag agt gtg tgc gat aag aac aag aat att gca ggg gac        835
Ser Asn Asp Lys Ser Val Cys Asp Lys Asn Lys Asn Ile Ala Gly Asp           
    205                 210                 215                           

tgg ggc ttc gac ccg gcg aaa tgg gca tat cag tat gat gag aag aat        883
Trp Gly Phe Asp Pro Ala Lys Trp Ala Tyr Gln Tyr Asp Glu Lys Asn           
220                 225                 230                 235           

aac aag ttc aac tat gtt ggt aag taa                                    910
Asn Lys Phe Asn Tyr Val Gly Lys                                           
                240                                                       


<210>  2
<211>  243
<212>  PRT
<213>  Aspergillus oryzae


<220>
<221>  SIGNAL
<222>  (1)..(22)

<220>
<221>  PROPEP
<222>  (23)..(37)

<220>
<221>  CHAIN
<222>  (38)..(243)
<223>  mature polypeptide

<400>  2

Met Gln Leu Thr Lys Ser Leu Leu Val Phe Ala Leu Tyr Met Phe Gly 
1               5                   10                  15      


Thr Gln His Val Leu Ala Val Pro Val Asn Pro Glu Pro Asp Ala Thr 
            20                  25                  30          


Ser Val Glu Asn Val Ala Leu Lys Thr Gly Ser Gly Asp Ser Gln Ser 
        35                  40                  45              


Asp Pro Ile Lys Ala Asp Leu Glu Val Lys Gly Gln Ser Ala Leu Pro 
    50                  55                  60                  


Phe Asp Val Asp Cys Trp Ala Ile Leu Cys Lys Gly Ala Pro Asn Val 
65                  70                  75                  80  


Leu Gln Arg Val Asn Glu Lys Thr Lys Asn Ser Asn Arg Asp Arg Ser 
                85                  90                  95      


Gly Ala Asn Lys Gly Pro Phe Lys Asp Pro Gln Lys Trp Gly Ile Lys 
            100                 105                 110         


Ala Leu Pro Pro Lys Asn Pro Ser Trp Ser Ala Gln Asp Phe Lys Ser 
        115                 120                 125             


Pro Glu Glu Tyr Ala Phe Ala Ser Ser Leu Gln Gly Gly Thr Asn Ala 
    130                 135                 140                 


Ile Leu Ala Pro Val Asn Leu Ala Ser Gln Asn Ser Gln Gly Gly Val 
145                 150                 155                 160 


Leu Asn Gly Phe Tyr Ser Ala Asn Lys Val Ala Gln Phe Asp Pro Ser 
                165                 170                 175     


Lys Pro Gln Gln Thr Lys Gly Thr Trp Phe Gln Ile Thr Lys Phe Thr 
            180                 185                 190         


Gly Ala Ala Gly Pro Tyr Cys Lys Ala Leu Gly Ser Asn Asp Lys Ser 
        195                 200                 205             


Val Cys Asp Lys Asn Lys Asn Ile Ala Gly Asp Trp Gly Phe Asp Pro 
    210                 215                 220                 


Ala Lys Trp Ala Tyr Gln Tyr Asp Glu Lys Asn Asn Lys Phe Asn Tyr 
225                 230                 235                 240 


Val Gly Lys 
            


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer oJAL316

<400>  3
gacggatcca ccatgcagct tactaagtcc ct                                     32


<210>  4
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer oJAL317

<400>  4
gacctcgagt tacttaccaa catagttgaa c                                      31


<210>  5
<211>  931
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR fragment

<400>  5
gacggatcca ccatgcagct tactaagtcc ctcctggtat tcgcgcttta catgtttggc       60

actcagcacg ttctagctgt gcctgtcaat cccgagcctg atgctacgag cgtcgaaaat      120

gttgccctta aaacaggcag cggtgatagc cagagcgatc ccatcaaggc ggacttggag      180

gtcaaaggcc aaagtgcttt gcctttcgac gtcgactgct gggctatcct gtgcaagggc      240

gccccgaatg tcctgtatgt cttcctttat tgaagctctt gatgtggctt gtatgtttga      300

ctaatatatc gcacccttag gcagcgcgtg aatgaaaaga cgaaaaatag taatcgcgat      360

cggagcggtg cgaacaaagg gcctttcaaa gatcctcaga aatggggcat caaagccctt      420

ccacctaaga atccatcctg gagcgcacaa gacttcaaat cacccgaaga atacgcattt      480

gcgtcttccc ttcaaggcgg aaccaagtat gctaagatca tcactgcttc aatcaatgtg      540

ttgttagctg actccgatgt gaccaagtgc catcctagcg cccgtcaacc tcgcttctca      600

gaactcccaa ggcggcgtct tgaacggttt ctactcggcg aacaaagtag cacaatttga      660

tcctagcaag ccccaacaga caaagggaac atggtttcag atcactaagt tcacaggtgc      720

agctgggtaa gaacttccag taccatggtc atatgcaatt tactaagaaa atactagtcc      780

ttactgcaag gctctgggga gtaatgataa gagtgtgtgc gataagaaca agaatattgc      840

aggggactgg ggcttcgacc cggcgaaatg ggcatatcag tatgatgaga agaataacaa      900

gttcaactat gttggtaagt aactcgaggt c                                     931


<210>  6
<211>  7
<212>  PRT
<213>  Aspergillus oryzae

<400>  6

Ala Leu Lys Thr Gly Ser Gly 
1               5           


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids

<400>  7

Lys Thr Gly Ser Gly Asp Ser 
1               5           


<210>  8
<211>  206
<212>  PRT
<213>  Aspergillus oryzae


<220>
<221>  CHAIN
<222>  (1)..(206)
<223>  mature polypeptide

<400>  8

Ala Leu Lys Thr Gly Ser Gly Asp Ser Gln Ser Asp Pro Ile Lys Ala 
1               5                   10                  15      


Asp Leu Glu Val Lys Gly Gln Ser Ala Leu Pro Phe Asp Val Asp Cys 
            20                  25                  30          


Trp Ala Ile Leu Cys Lys Gly Ala Pro Asn Val Leu Gln Arg Val Asn 
        35                  40                  45              


Glu Lys Thr Lys Asn Ser Asn Arg Asp Arg Ser Gly Ala Asn Lys Gly 
    50                  55                  60                  


Pro Phe Lys Asp Pro Gln Lys Trp Gly Ile Lys Ala Leu Pro Pro Lys 
65                  70                  75                  80  


Asn Pro Ser Trp Ser Ala Gln Asp Phe Lys Ser Pro Glu Glu Tyr Ala 
                85                  90                  95      


Phe Ala Ser Ser Leu Gln Gly Gly Thr Asn Ala Ile Leu Ala Pro Val 
            100                 105                 110         


Asn Leu Ala Ser Gln Asn Ser Gln Gly Gly Val Leu Asn Gly Phe Tyr 
        115                 120                 125             


Ser Ala Asn Lys Val Ala Gln Phe Asp Pro Ser Lys Pro Gln Gln Thr 
    130                 135                 140                 


Lys Gly Thr Trp Phe Gln Ile Thr Lys Phe Thr Gly Ala Ala Gly Pro 
145                 150                 155                 160 


Tyr Cys Lys Ala Leu Gly Ser Asn Asp Lys Ser Val Cys Asp Lys Asn 
                165                 170                 175     


Lys Asn Ile Ala Gly Asp Trp Gly Phe Asp Pro Ala Lys Trp Ala Tyr 
            180                 185                 190         


Gln Tyr Asp Glu Lys Asn Asn Lys Phe Asn Tyr Val Gly Lys 
        195                 200                 205     


<210>  9
<211>  204
<212>  PRT
<213>  Aspergillus oryzae


<220>
<221>  CHAIN
<222>  (1)..(204)
<223>  mature polypeptide

<400>  9

Lys Thr Gly Ser Gly Asp Ser Gln Ser Asp Pro Ile Lys Ala Asp Leu 
1               5                   10                  15      


Glu Val Lys Gly Gln Ser Ala Leu Pro Phe Asp Val Asp Cys Trp Ala 
            20                  25                  30          


Ile Leu Cys Lys Gly Ala Pro Asn Val Leu Gln Arg Val Asn Glu Lys 
        35                  40                  45              


Thr Lys Asn Ser Asn Arg Asp Arg Ser Gly Ala Asn Lys Gly Pro Phe 
    50                  55                  60                  


Lys Asp Pro Gln Lys Trp Gly Ile Lys Ala Leu Pro Pro Lys Asn Pro 
65                  70                  75                  80  


Ser Trp Ser Ala Gln Asp Phe Lys Ser Pro Glu Glu Tyr Ala Phe Ala 
                85                  90                  95      


Ser Ser Leu Gln Gly Gly Thr Asn Ala Ile Leu Ala Pro Val Asn Leu 
            100                 105                 110         


Ala Ser Gln Asn Ser Gln Gly Gly Val Leu Asn Gly Phe Tyr Ser Ala 
        115                 120                 125             


Asn Lys Val Ala Gln Phe Asp Pro Ser Lys Pro Gln Gln Thr Lys Gly 
    130                 135                 140                 


Thr Trp Phe Gln Ile Thr Lys Phe Thr Gly Ala Ala Gly Pro Tyr Cys 
145                 150                 155                 160 


Lys Ala Leu Gly Ser Asn Asp Lys Ser Val Cys Asp Lys Asn Lys Asn 
                165                 170                 175     


Ile Ala Gly Asp Trp Gly Phe Asp Pro Ala Lys Trp Ala Tyr Gln Tyr 
            180                 185                 190         


Asp Glu Lys Asn Asn Lys Phe Asn Tyr Val Gly Lys 
        195                 200                 


<210>  10
<211>  269
<212>  PRT
<213>  Bacillus lentus

<400>  10

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  11
<211>  142
<212>  PRT
<213>  Bacillus licheniformis


<220>
<221>  SIGNAL
<222>  (1)..(33)

<220>
<221>  CHAIN
<222>  (34)..(142)
<223>  mature polypeptide

<400>  11

Met Ile Lys Lys Trp Ala Val His Leu Leu Phe Ser Ala Leu Val Leu 
1               5                   10                  15      


Leu Gly Leu Ser Gly Gly Ala Ala Tyr Ser Pro Gln His Ala Glu Gly 
            20                  25                  30          


Ala Ala Arg Tyr Asp Asp Ile Leu Tyr Phe Pro Ala Ser Arg Tyr Pro 
        35                  40                  45              


Glu Thr Gly Ala His Ile Ser Asp Ala Ile Lys Ala Gly His Ser Asp 
    50                  55                  60                  


Val Cys Thr Ile Glu Arg Ser Gly Ala Asp Lys Arg Arg Gln Glu Ser 
65                  70                  75                  80  


Leu Lys Gly Ile Pro Thr Lys Pro Gly Phe Asp Arg Asp Glu Trp Pro 
                85                  90                  95      


Met Ala Met Cys Glu Glu Gly Gly Lys Gly Ala Ser Val Arg Tyr Val 
            100                 105                 110         


Ser Ser Ser Asp Asn Arg Gly Ala Gly Ser Trp Val Gly Asn Arg Leu 
        115                 120                 125             


Ser Gly Phe Ala Asp Gly Thr Arg Ile Leu Phe Ile Val Gln 
    130                 135                 140         


<210>  12
<211>  732
<212>  DNA
<213>  Aspergillus oryzae

<400>  12
atgcagctta ctaagtccct cctggtattc gcgctttaca tgtttggcac tcagcacgtt       60

ctagctgtgc ctgtcaatcc cgagcctgat gctacgagcg tcgaaaatgt tgcccttaaa      120

acaggcagcg gtgatagcca gagcgatccc atcaaggcgg acttggaggt caaaggccaa      180

agtgctttgc ctttcgacgt cgactgctgg gctatcctgt gcaagggcgc cccgaatgtc      240

ctgcagcgcg tgaatgaaaa gacgaaaaat agtaatcgcg atcggagcgg tgcgaacaaa      300

gggcctttca aagatcctca gaaatggggc atcaaagccc ttccacctaa gaatccatcc      360

tggagcgcac aagacttcaa atcacccgaa gaatacgcat ttgcgtcttc ccttcaaggc      420

ggaaccaatg ccatcctagc gcccgtcaac ctcgcttctc agaactccca aggcggcgtc      480

ttgaacggtt tctactcggc gaacaaagta gcacaatttg atcctagcaa gccccaacag      540

acaaagggaa catggtttca gatcactaag ttcacaggtg cagctggtcc ttactgcaag      600

gctctgggga gtaatgataa gagtgtgtgc gataagaaca agaatattgc aggggactgg      660

ggcttcgacc cggcgaaatg ggcatatcag tatgatgaga agaataacaa gttcaactat      720

gttggtaagt aa                                                          732


