
                                SEQUENCE LISTING

<110> Jensen, Michael C.
      PUn, Suzie
      Kacherovsky, Nataly

<120> PRODUCTION OF ENGINEERED T-CELLS BY
  SLEEPING BEAUTY TRANSPOSON COUPLED WITH METHOTREXATE
  SELECTION

<130> SCRI.077PR

<140> 62/090,845          
<141> 2014-12-11  

<160> 7

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 352
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic DNA transposon of copied inverted repeat
      in description from Tanichthys albonubes

<400> 1
cagttgaagt cggaagttta catacactta agttggagtc attaaaactc gtttttcaac 60
tactccacaa atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact 120
ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata 180
attcactgta tcacaattcc agtgggtcag aagtttacat acactaagtt gactgtgcct 240
ttaaacagct tggaaaattc cagaaaatga tgtcatggct ttagaagctt ctgatagact 300
aattgacatc atttgagtca attggaggtg tacctgtgga tgtatttcaa gg         352

<210> 2
<211> 564
<212> DNA
<213> Artificial Sequence

<220> 
<223> Human protein with dihydrofolate reductase double
      mutation

<400> 2
atggttggtt cgctaaactg catcgtcgct gtgtcccaga acatgggcat cggcaagaac 60
ggggacttcc cctggccacc gctcaggaat gaatccagat atttccagag aatgaccaca 120
acctcttcag tagaaggtaa acagaatctg gtgattatgg gtaagaagac ctggttctcc 180
attcctgaga agaatcgacc tttaaagggt agaattaatt tagttctcag cagagaactc 240
aaggaacctc cacaaggagc tcattttctt tccagaagtc tagatgatgc cttaaaactt 300
actgaacaac cagaattagc aaataaagta gacatggtct ggatagttgg tggcagttct 360
gtttataagg aagccatgaa tcacccaggc catcttaaac tatttgtgac aaggatcatg 420
caagactttg aaagtgacac gttttttcca gaaattgatt tggagaaata taaacttctg 480
ccagaatacc caggtgttct ctctgatgtc caggaggaga aaggcattaa gtacaaattt 540
gaagtatatg agaagaatga ttaa                                        564

<210> 3
<211> 187
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human protein with dihydrofolate reductase double
      mutation

<400> 3
Met Val Gly Ser Leu Asn Cys Ile Val Ala Val Ser Gln Asn Met Gly
 1               5                  10                  15      
Ile Gly Lys Asn Gly Asp Phe Pro Trp Pro Pro Leu Arg Asn Glu Ser
            20                  25                  30          
Arg Tyr Phe Gln Arg Met Thr Thr Thr Ser Ser Val Glu Gly Lys Gln
        35                  40                  45              
Asn Leu Val Ile Met Gly Lys Lys Thr Trp Phe Ser Ile Pro Glu Lys
    50                  55                  60                  
Asn Arg Pro Leu Lys Gly Arg Ile Asn Leu Val Leu Ser Arg Glu Leu
65                  70                  75                  80  
Lys Glu Pro Pro Gln Gly Ala His Phe Leu Ser Arg Ser Leu Asp Asp
                85                  90                  95      
Ala Leu Lys Leu Thr Glu Gln Pro Glu Leu Ala Asn Lys Val Asp Met
            100                 105                 110         
Val Trp Ile Val Gly Gly Ser Ser Val Tyr Lys Glu Ala Met Asn His
        115                 120                 125             
Pro Gly His Leu Lys Leu Phe Val Thr Arg Ile Met Gln Asp Phe Glu
    130                 135                 140                 
Ser Asp Thr Phe Phe Pro Glu Ile Asp Leu Glu Lys Tyr Lys Leu Leu
145                 150                 155                 160 
Pro Glu Tyr Pro Gly Val Leu Ser Asp Val Gln Glu Glu Lys Gly Ile
                165                 170                 175     
Lys Tyr Lys Phe Glu Val Tyr Glu Lys Asn Asp
            180                 185         


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 4
acaagatcat ccgcagcaac                                             20

<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 5
ttgaagtgca tgtggctgtc                                             20

<210> 6
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 6
acaactttgg tatcgtggaa gg                                          22

<210> 7
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 7
gccatcacgc cacagtttc                                              19
