
                                SEQUENCE LISTING

<110> Seattle Children's Hospital
      dba Seattle Children's Research Institute
      Jensen, Michael C.

<120> DRUG REGULATED TRANSGENE EXPRESSION
  

<130> SCRI.053WO

<150> 62/058,973          
<151> 2014-10-02  

<150> 61/977,751          
<151> 2014-04-10  

<150> 61/986,479          
<151> 2014-04-30  

<150> 62/089,730          
<151> 2014-12-09  

<150> 62/090,845          
<151> 2014-12-11  

<150> 62/088,363          
<151> 2014-12-05  

<160> 94

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2528
<212> DNA
<213> Artificial Sequence

<220> 
<223> GMCSFss-Her2scFv-IgG4hinge-CD28tm-41BB-Zeta-T2A-EG
      FRt

<400> 1
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg 60
atccccgata tccagatgac ccagtccccg agctccctgt ccgcctctgt gggcgatagg 120
gtcaccatca cctgccgtgc cagtcaggat gtgaatactg ctgtagcctg gtatcaacag 180
aaaccaggaa aagctccgaa actactgatt tactcggcat ccttcctcta ctctggagtc 240
ccttctcgct tctctggttc cagatctggg acggatttca ctctgaccat cagcagtctg 300
cagccggaag acttcgcaac ttattactgt cagcaacatt atactactcc tcccacgttc 360
ggacagggta ccaaggtgga gatcaaaggc agtactagcg gcggtggctc cgggggcgga 420
tccggtgggg gcggcagcag cgaggttcag ctggtggagt ctggcggtgg cctggtgcag 480
ccagggggct cactccgttt gtcctgtgca gcttctggct tcaacattaa agacacctat 540
atacactggg tgcgtcaggc cccgggtaag ggcctggaat gggttgcaag gatttatcct 600
acgaatggtt atactagata tgccgatagc gtcaagggcc gtttcactat aagcgcagac 660
acatccaaaa acacagccta cctgcagatg aacagcctgc gtgctgagga cactgccgtc 720
tattattgtt ctagatgggg aggggacggc ttctatgcta tggactactg gggtcaagga 780
accctggtca ccgtctcgag gaatctaagt acggaccgcc ctgcccccct tgccctatgt 840
tctgggtgct ggtggtggtc ggaggcgtgc tggcctgcta cagcctgctg gtcaccgtgg 900
ccttcatcat cttttgggtg aaacggggca gaaagaaact cctgtatata ttcaaacaac 960
catttatgag accagtacaa actactcaag aggaagatgg ctgtagctgc cgatttccag 1020
aagaagaaga aggaggatgt gaactgcggg tgaagttcag cagaagcgcc gacgcccctg 1080
cctaccagca gggccagaat cagctgtaca acgagctgaa cctgggcaga agggaagagt 1140
acgacgtcct ggataagcgg agaggccggg accctgagat gggcggcaag cctcggcgga 1200
agaaccccca ggaaggcctg tataacgaac tgcagaaaga caagatggcc gaggcctaca 1260
gcgagatcgg catgaagggc gagcggaggc ggggcaaggg ccacgacggc ctgtatcagg 1320
gcctgtccac cgccaccaag gatacctacg acgccctgca catgcaggcc ctgcccccaa 1380
ggctcgaggg cggcggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga 1440
atcccggccc taggatgctt ctcctggtga caagccttct gctctgtgag ttaccacacc 1500
cagcattcct cctgatccca cgcaaagtgt gtaacggaat aggtattggt gaatttaaag 1560
actcactctc cataaatgct acgaatatta aacacttcaa aaactgcacc tccatcagtg 1620
gcgatctcca catcctgccg gtggcattta ggggtgactc cttcacacat actcctcctc 1680
tggatccaca ggaactggat attctgaaaa ccgtaaagga aatcacaggg tttttgctga 1740
ttcaggcttg gcctgaaaac aggacggacc tccatgcctt tgagaaccta gaaatcatac 1800
gcggcaggac caagcaacat ggtcagtttt ctcttgcagt cgtcagcctg aacataacat 1860
ccttgggatt acgctccctc aaggagataa gtgatggaga tgtgataatt tcaggaaaca 1920
aaaatttgtg ctatgcaaat acaataaact ggaaaaaact gtttgggacc tccggtcaga 1980
aaaccaaaat tataagcaac agaggtgaaa acagctgcaa ggccacaggc caggtctgcc 2040
atgccttgtg ctcccccgag ggctgctggg gcccggagcc cagggactgc gtctcttgcc 2100
ggaatgtcag ccgaggcagg gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa 2160
gggagtttgt ggagaactct gagtgcatac agtgccaccc agagtgcctg cctcaggcca 2220
tgaacatcac ctgcacagga cggggaccag acaactgtat ccagtgtgcc cactacattg 2280
acggccccca ctgcgtcaag acctgcccgg caggagtcat gggagaaaac aacaccctgg 2340
tctggaagta cgcagacgcc ggccatgtgt gccacctgtg ccatccaaac tgcacctacg 2400
gatgcactgg gccaggtctt gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg 2460
ccactgggat ggtgggggcc ctcctcttgc tgctggtggt ggccctgggg atcggcctct 2520
tcatgtga                                                          2528

<210> 2
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> GMCSFRss

<400> 2
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg 60
atcccc                                                            66

<210> 3
<211> 735
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD19scFv DNA

<400> 3
gacatccaga tgacccagac cacctccagc ctgagcgcca gcctgggcga ccgggtgacc 60
atcagctgcc gggccagcca ggacatcagc aagtacctga actggtatca gcagaagccc 120
gacggcaccg tcaagctgct gatctaccac accagccggc tgcacagcgg cgtgcccagc 180
cggtttagcg gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag 240
gaagatatcg ccacctactt ttgccagcag ggcaacacac tgccctacac ctttggcggc 300
ggaacaaagc tggaaatcac cggcagcacc tccggcagcg gcaagcctgg cagcggcgag 360
ggcagcacca agggcgaggt gaagctgcag gaaagcggcc ctggcctggt ggcccccagc 420
cagagcctga gcgtgacctg caccgtgagc ggcgtgagcc tgcccgacta cggcgtgagc 480
tggatccggc agccccccag gaagggcctg gaatggctgg gcgtgatctg gggcagcgag 540
accacctact acaacagcgc cctgaagagc cggctgacca tcatcaagga caacagcaag 600
agccaggtgt tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc 660
gccaagcact actactacgg cggcagctac gccatggact actggggcca gggcaccagc 720
gtgaccgtga gcagc                                                  735

<210> 4
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG4hinge

<400> 4
gaatctaagt acggaccgcc ctgcccccct tgccct                           36

<210> 5
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD28 transmembrane domain

<400> 5
atgttctggg tgctggtggt ggtcggaggc gtgctggcct gctacagcct gctggtcacc 60
gtggccttca tcatcttttg ggtg                                        84

<210> 6
<211> 126
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA encoding  41BB domain

<400> 6
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa 60
actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt 120
gaactg                                                            126

<210> 7
<211> 336
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA encoding CD3Zeta domain

<400> 7
cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca gaatcagctg 60
tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa gcggagaggc 120
cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg cctgtataac 180
gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg 240
aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac caaggatacc 300
tacgacgccc tgcacatgca ggccctgccc ccaagg                           336

<210> 8
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> Self cleaving T2A

<400> 8
ctcgagggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 60
cccggcccta gg                                                     72

<210> 9
<211> 1074
<212> DNA
<213> Artificial Sequence

<220> 
<223> EGFRt

<400> 9
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata 120
aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc 180
ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa 240
ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 300
gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag 360
caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc 420
tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat 480
gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata 540
agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 600
cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga 660
ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag 720
aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc 780
acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc 840
gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 900
gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca 960
ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg 1020
ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gtga       1074

<210> 10
<211> 2529
<212> DNA
<213> Artificial Sequence

<220> 
<223> Protein that has domains linked as such GMCSFRss -
      CD19scFv  - IgG4hinge - CD28tm  - 41BB - CD3Zeta -
      T2A - EGFRt

<400> 10
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg 60
atccccgaca tccagatgac ccagaccacc tccagcctga gcgccagcct gggcgaccgg 120
gtgaccatca gctgccgggc cagccaggac atcagcaagt acctgaactg gtatcagcag 180
aagcccgacg gcaccgtcaa gctgctgatc taccacacca gccggctgca cagcggcgtg 240
cccagccggt ttagcggcag cggctccggc accgactaca gcctgaccat ctccaacctg 300
gaacaggaag atatcgccac ctacttttgc cagcagggca acacactgcc ctacaccttt 360
ggcggcggaa caaagctgga aatcaccggc agcacctccg gcagcggcaa gcctggcagc 420
ggcgagggca gcaccaaggg cgaggtgaag ctgcaggaaa gcggccctgg cctggtggcc 480
cccagccaga gcctgagcgt gacctgcacc gtgagcggcg tgagcctgcc cgactacggc 540
gtgagctgga tccggcagcc ccccaggaag ggcctggaat ggctgggcgt gatctggggc 600
agcgagacca cctactacaa cagcgccctg aagagccggc tgaccatcat caaggacaac 660
agcaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac 720
tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc 780
accagcgtga ccgtgagcag cgagagcaag tacggaccgc cctgcccccc ttgccctatg 840
ttctgggtgc tggtggtggt cggaggcgtg ctggcctgct acagcctgct ggtcaccgtg 900
gccttcatca tcttttgggt gaaacggggc agaaagaaac tcctgtatat attcaaacaa 960
ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca 1020
gaagaagaag aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct 1080
gcctaccagc agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag 1140
tacgacgtcc tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg 1200
aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac 1260
agcgagatcg gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag 1320
ggcctgtcca ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca 1380
aggctcgagg gcggcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag 1440
aatcccggcc ctaggatgct tctcctggtg acaagccttc tgctctgtga gttaccacac 1500
ccagcattcc tcctgatccc acgcaaagtg tgtaacggaa taggtattgg tgaatttaaa 1560
gactcactct ccataaatgc tacgaatatt aaacacttca aaaactgcac ctccatcagt 1620
ggcgatctcc acatcctgcc ggtggcattt aggggtgact ccttcacaca tactcctcct 1680
ctggatccac aggaactgga tattctgaaa accgtaaagg aaatcacagg gtttttgctg 1740
attcaggctt ggcctgaaaa caggacggac ctccatgcct ttgagaacct agaaatcata 1800
cgcggcagga ccaagcaaca tggtcagttt tctcttgcag tcgtcagcct gaacataaca 1860
tccttgggat tacgctccct caaggagata agtgatggag atgtgataat ttcaggaaac 1920
aaaaatttgt gctatgcaaa tacaataaac tggaaaaaac tgtttgggac ctccggtcag 1980
aaaaccaaaa ttataagcaa cagaggtgaa aacagctgca aggccacagg ccaggtctgc 2040
catgccttgt gctcccccga gggctgctgg ggcccggagc ccagggactg cgtctcttgc 2100
cggaatgtca gccgaggcag ggaatgcgtg gacaagtgca accttctgga gggtgagcca 2160
agggagtttg tggagaactc tgagtgcata cagtgccacc cagagtgcct gcctcaggcc 2220
atgaacatca cctgcacagg acggggacca gacaactgta tccagtgtgc ccactacatt 2280
gacggccccc actgcgtcaa gacctgcccg gcaggagtca tgggagaaaa caacaccctg 2340
gtctggaagt acgcagacgc cggccatgtg tgccacctgt gccatccaaa ctgcacctac 2400
ggatgcactg ggccaggtct tgaaggctgt ccaacgaatg ggcctaagat cccgtccatc 2460
gccactggga tggtgggggc cctcctcttg ctgctggtgg tggccctggg gatcggcctc 2520
ttcatgtga                                                         2529

<210> 11
<211> 842
<212> PRT
<213> Artificial Sequence

<220> 
<223> GMCSFRss - CD19scFv  - IgG4hinge - CD28tm  - 41BB
      - CD3Zeta - T2A - EGFRt

<400> 11
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser
            20                  25                  30          
Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser
        35                  40                  45              
Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly
    50                  55                  60                  
Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val
65                  70                  75                  80  
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr
                85                  90                  95      
Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln
            100                 105                 110         
Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile
        115                 120                 125             
Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser
    130                 135                 140                 
Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala
145                 150                 155                 160 
Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu
                165                 170                 175     
Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu
            180                 185                 190         
Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser
        195                 200                 205             
Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln
    210                 215                 220                 
Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr
225                 230                 235                 240 
Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr
                245                 250                 255     
Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly
            260                 265                 270         
Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly
        275                 280                 285             
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile
    290                 295                 300                 
Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln
305                 310                 315                 320 
Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser
                325                 330                 335     
Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys
            340                 345                 350         
Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln
        355                 360                 365             
Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu
    370                 375                 380                 
Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg
385                 390                 395                 400 
Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met
                405                 410                 415     
Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly
            420                 425                 430         
Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp
        435                 440                 445             
Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly
    450                 455                 460                 
Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu
465                 470                 475                 480 
Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys
                485                 490                 495     
Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn
            500                 505                 510         
Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr
        515                 520                 525             
Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His
    530                 535                 540                 
Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro
545                 550                 555                 560 
Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr
                565                 570                 575     
Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His
            580                 585                 590         
Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly
        595                 600                 605             
Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu
    610                 615                 620                 
Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn
625                 630                 635                 640 
Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly
                645                 650                 655     
Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser
            660                 665                 670         
Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly
        675                 680                 685             
Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser
    690                 695                 700                 
Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro
705                 710                 715                 720 
Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys
                725                 730                 735     
Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn
            740                 745                 750         
Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr
        755                 760                 765             
Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr
    770                 775                 780                 
Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr
785                 790                 795                 800 
Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys
                805                 810                 815     
Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu
            820                 825                 830         
Val Val Ala Leu Gly Ile Gly Leu Phe Met
        835                 840         


<210> 12
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> (G4S)3 linker

<400> 12
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
 1               5                  10                  15  


<210> 13
<211> 327
<212> PRT
<213> Artificial Sequence

<220> 
<223> Uniprot P0861 IgG4-Fc

<400> 13
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg
 1               5                  10                  15      
Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr
65                  70                  75                  80  
Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys
                85                  90                  95      
Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro
            100                 105                 110         
Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
        115                 120                 125             
Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
    130                 135                 140                 
Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp
145                 150                 155                 160 
Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe
                165                 170                 175     
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
            180                 185                 190         
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu
        195                 200                 205             
Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
    210                 215                 220                 
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
225                 230                 235                 240 
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
                245                 250                 255     
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
            260                 265                 270         
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
        275                 280                 285             
Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
    290                 295                 300                 
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
305                 310                 315                 320 
Leu Ser Leu Ser Leu Gly Lys
                325         


<210> 14
<211> 220
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28 transmembrane domain

<400> 14
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val
 1               5                  10                  15      
Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr
            20                  25                  30          
Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser
        35                  40                  45              
Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu
    50                  55                  60                  
Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser
65                  70                  75                  80  
Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr
                85                  90                  95      
Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys
            100                 105                 110         
Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser
        115                 120                 125             
Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro
    130                 135                 140                 
Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly
145                 150                 155                 160 
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile
                165                 170                 175     
Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met
            180                 185                 190         
Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro
        195                 200                 205             
Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser
    210                 215                 220 


<210> 15
<211> 240
<212> PRT
<213> Artificial Sequence

<220> 
<223> 4-1BB domain

<400> 15
Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu
 1               5                  10                  15      
Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro
            20                  25                  30          
Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys
        35                  40                  45              
Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile
    50                  55                  60                  
Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser
65                  70                  75                  80  
Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly
                85                  90                  95      
Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu
            100                 105                 110         
Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln
        115                 120                 125             
Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys
    130                 135                 140                 
Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro
145                 150                 155                 160 
Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala
                165                 170                 175     
Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu
            180                 185                 190         
Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu
        195                 200                 205             
Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe
    210                 215                 220                 
Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly
225                 230                 235                 240 


<210> 16
<211> 164
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human CD3 Zeta isoform 3

<400> 16
Met Lys Trp Lys Ala Leu Phe Thr Ala Ala Ile Leu Gln Ala Gln Leu
 1               5                  10                  15      
Pro Ile Thr Glu Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys
            20                  25                  30          
Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu Thr Ala
        35                  40                  45              
Leu Phe Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr
    50                  55                  60                  
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg
65                  70                  75                  80  
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met
                85                  90                  95      
Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn
            100                 105                 110         
Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met
        115                 120                 125             
Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly
    130                 135                 140                 
Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala
145                 150                 155                 160 
Leu Pro Pro Arg
                


<210> 17
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG1

<400> 17
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro
 1               5                  10                  15  


<210> 18
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG2

<400> 18
Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro
 1               5                  10          


<210> 19
<211> 61
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG3

<400> 19
Glu Leu Lys Thr Pro Leu Gly Asp Thr His Thr Cys Pro Arg Cys Pro
 1               5                  10                  15      
Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu
            20                  25                  30          
Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu Pro
        35                  40                  45              
Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro
    50                  55                  60      


<210> 20
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG4

<400> 20
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro
 1               5                  10          


<210> 21
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified human IgG4

<400> 21
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10          


<210> 22
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02649

<400> 22
atcaaaagaa tagaccgaga tagggt                                      26

<210> 23
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02648

<400> 23
ccgtaccttt aagaccaatg acttac                                      26

<210> 24
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02650

<400> 24
ttgagagttt tcgccccg                                               18

<210> 25
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02651

<400> 25
aatagacaga tcgctgagat aggt                                        24

<210> 26
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02652

<400> 26
caggtatccg gtaagcgg                                               18

<210> 27
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02653

<400> 27
cgaccagcaa ccatagtcc                                              19

<210> 28
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02654

<400> 28
tagcggtttg actcacgg                                               18

<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02655

<400> 29
gcagggagct agaacgattc                                             20

<210> 30
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02656

<400> 30
attgtctggt atagtgcagc ag                                          22

<210> 31
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02657

<400> 31
tcgcaacggg tttgcc                                                 16

<210> 32
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02658

<400> 32
aggaagatat cgccacctac t                                           21

<210> 33
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02601

<400> 33
cgggtgaagt tcagcagaag                                             20

<210> 34
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02735

<400> 34
actgtgtttg ctgacgcaac                                             20

<210> 35
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> oJ02715

<400> 35
atgcttctcc tggtgacaag                                             20

<210> 36
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified Human IgG4 linker

<400> 36
Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  


<210> 37
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified Human IgG4

<400> 37
Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10  


<210> 38
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified Human IgG4

<400> 38
Glu Val Val Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10              


<210> 39
<211> 2586
<212> DNA
<213> Artificial Sequence

<220> 
<223> TamR-tf(HEA3)

<400> 39
atggtgtcca agctgtccca gctgcagaca gaactgctgg cagcactgct ggaaagcggc 60
ctgagcaaag aggccctgat tcaggcactc ggcgaacctg gaccttatct gctcgctggc 120
gaaggccctc tggataaggg cgagagctgt ggcggaggaa gaggagagct ggccgagctg 180
cctaacggcc tgggcgagac aagaggcagc gaggacgaga cagacgacga cggcgaggac 240
ttcacccccc ccatcctgaa agagctggaa aacctgagcc ccgaggaagc cgcccaccag 300
aaagccgtgg tggagacact gctgcaggaa gatccctggc gggtcgccaa gatggtcaag 360
agctacctgc agcagcacaa catcccccag cgggaggtgg tggacaccac cggcctgaac 420
cagagccacc tgagccagca cctgaacaag ggcaccccca tgaaaaccca gaagagagcc 480
gccctgtaca cttggtacgt gcggaagcag agagaggtgg cccagcagtt tacacacgcc 540
ggccagggcg gcctgatcga ggaacctacc ggcgacgagc tgcccaccaa gaagggcaga 600
cggacccggt ttaagtgggg ccctgcatct cagcagatcc tgttccaggc ctacgagcgg 660
cagaagaacc ccagcaaaga ggaacgggag acactggtgg aagagtgcaa ccgggccgag 720
tgcatccaga gaggcgtgag cccttctcag gctcagggcc tcggcagcaa tctggtcacc 780
gaagtgcggg tgtacaattg gttcgccaac cggcggaaag aggaagcctt ccggcacaag 840
ctgtctgctg gcgatatgag agccgccaac ctgtggccca gccccctgat gatcaagcgg 900
agcaagaaga acagcctggc cctgagcctg accgccgatc agatggtgtc cgctctgctg 960
gacgccgagc cccctatcct gtacagcgag tacgacccca ccagaccctt cagcgaggcc 1020
agcatgatgg gcctgctgac caacctggcc gaccgggagc tggtgcacat gatcaactgg 1080
gccaagcggg tgcccggctt cgtggacctg accctgcacg accaggtcca cctgctggaa 1140
tgtgcctggc tggaaatcct gatgatcggc ctcgtgtgga gaagcatgga acaccccggc 1200
aagctgctgt tcgcccccaa cctgctcctg gaccggaacc agggaaagtg cgtggagggc 1260
atggtggaga tcttcgacat gctgctggcc acctccagcc ggttccggat gatgaacctg 1320
cagggcgagg aattcgtgtg cctgaagtcc atcatcctgc tgaacagcgg cgtgtacacc 1380
ttcctgtcat ccaccctgaa gtccctggaa gagaaggacc acatccaccg ggtgctggac 1440
aagatcaccg acaccctgat ccacctgatg gccaaggctg gcctgacact ccagcagcag 1500
caccagagac tggcccagct gctgctgatc ctgagccaca tccggcacat gagcaacaag 1560
cggatggaac acctgtacag catgaagtgc aagaacgtgg tgcccctgta cgacctgctg 1620
ctcgagatgc tggatgccca cagactgcac gcccctacaa gcagaggcgg agccagcgtg 1680
gaggaaaccg accagtctca cctggccacc gccggcagca caagcagcca cagcctgcag 1740
aagtactaca tcaccggcga ggccgaggga ttccctgcca ccgtggagtt ccagtacctg 1800
cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta cgagacattc 1860
aagagcatca tgaagaagtc ccccttcagc ggccccaccg atcccagacc cccccctaga 1920
agaatcgccg tgcccagcag atctagcgcc agcgtgccca agcctgcccc ccagccctac 1980
cctttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat ggtgttcccc 2040
agcggccaga tctctcaggc ctctgctctg gcacctgctc cacctcaggt gctgcctcag 2100
gcccctgctc cagccccagc ccctgccatg gtgtctgcac tggcccaggc tccagctcct 2160
gtgcctgtgc tggcccctgg acctcctcag gctgtggccc ctcctgcccc taaacctacc 2220
caggccgggg agggaacact gtctgaggcc ctgctgcagc tccagttcga cgacgaggat 2280
ctgggagcac tgctgggcaa tagcaccgac cccgccgtgt ttaccgacct ggcctccgtg 2340
gacaacagcg agttccagca gctcctcaac cagggcatcc ctgtcgcccc acacaccacc 2400
gagcccatgc tgatggaata ccccgaggcc atcaccagac tggtcacagg cgcccagagg 2460
cctccagatc cagcaccagc tccactggga gcccctggcc tgcctaatgg gctgctgtct 2520
ggcgacgagg acttctccag cattgccgac atggacttca gcgccctgct gtcccagatc 2580
agcagc                                                            2586

<210> 40
<211> 828
<212> PRT
<213> Artificial Sequence

<220> 
<223> TamR-tf(HEA3)

<400> 40
Met Val Ser Lys Leu Ser Gln Leu Gln Thr Glu Leu Leu Ala Ala Leu
 1               5                  10                  15      
Leu Glu Ser Gly Leu Ser Lys Glu Ala Leu Ile Gln Ala Leu Gly Glu
            20                  25                  30          
Pro Gly Pro Tyr Leu Leu Ala Gly Glu Gly Pro Leu Asp Lys Gly Glu
        35                  40                  45              
Ser Cys Gly Gly Gly Arg Gly Glu Leu Ala Glu Leu Pro Asn Gly Leu
    50                  55                  60                  
Gly Glu Thr Arg Gly Ser Glu Asp Glu Thr Asp Asp Asp Gly Glu Asp
65                  70                  75                  80  
Phe Thr Pro Pro Ile Leu Lys Glu Leu Glu Asn Leu Ser Pro Glu Glu
                85                  90                  95      
Ala Ala His Gln Lys Ala Val Val Glu Thr Leu Leu Gln Glu Asp Pro
            100                 105                 110         
Trp Arg Val Ala Lys Met Val Lys Ser Tyr Leu Gln Gln His Asn Ile
        115                 120                 125             
Pro Gln Arg Glu Val Val Asp Thr Thr Gly Leu Asn Gln Ser His Leu
    130                 135                 140                 
Ser Gln His Leu Asn Lys Gly Thr Pro Met Lys Thr Gln Lys Arg Ala
145                 150                 155                 160 
Ala Leu Tyr Thr Trp Tyr Val Arg Lys Gln Arg Glu Val Ala Gln Gln
                165                 170                 175     
Phe Thr His Ala Gly Gln Gly Gly Leu Ile Glu Glu Pro Thr Gly Asp
            180                 185                 190         
Glu Leu Pro Thr Lys Lys Gly Arg Arg Asn Arg Phe Lys Trp Gly Pro
        195                 200                 205             
Ala Ser Gln Gln Ile Leu Phe Gln Ala Tyr Glu Arg Gln Lys Asn Pro
    210                 215                 220                 
Ser Lys Glu Glu Arg Glu Thr Leu Val Glu Glu Cys Asn Arg Ala Glu
225                 230                 235                 240 
Cys Ile Gln Arg Gly Val Ser Pro Ser Gln Ala Gln Gly Leu Gly Ser
                245                 250                 255     
Asn Leu Val Thr Glu Val Arg Val Tyr Asn Trp Phe Ala Asn Arg Arg
            260                 265                 270         
Lys Glu Glu Ala Phe Arg His Lys Leu Ser Ala Gly Asp Met Arg Ala
        275                 280                 285             
Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn
    290                 295                 300                 
Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu
305                 310                 315                 320 
Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro
                325                 330                 335     
Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg
            340                 345                 350         
Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val
        355                 360                 365             
Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu
    370                 375                 380                 
Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly
385                 390                 395                 400 
Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys
                405                 410                 415     
Cys Val Glu Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser
            420                 425                 430         
Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu
        435                 440                 445             
Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser
    450                 455                 460                 
Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp
465                 470                 475                 480 
Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr
                485                 490                 495     
Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser
            500                 505                 510         
His Ile Arg His Met Ser Asn Lys Arg Met Glu His Leu Tyr Ser Met
        515                 520                 525             
Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu
    530                 535                 540                 
Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val
545                 550                 555                 560 
Glu Glu Phe Gln Tyr Leu Pro Asp Thr Asp Asp Arg His Arg Ile Glu
                565                 570                 575     
Glu Lys Arg Lys Arg Thr Tyr Glu Thr Phe Lys Ser Ile Met Lys Lys
            580                 585                 590         
Ser Pro Phe Ser Gly Pro Thr Asp Pro Arg Pro Pro Pro Arg Arg Ile
        595                 600                 605             
Ala Val Pro Ser Arg Ser Ser Ala Ser Val Pro Lys Pro Ala Pro Gln
    610                 615                 620                 
Pro Tyr Pro Phe Thr Ser Ser Leu Ser Thr Ile Asn Tyr Asp Glu Phe
625                 630                 635                 640 
Pro Thr Met Val Phe Pro Ser Gly Gln Ile Ser Gln Ala Ser Ala Leu
                645                 650                 655     
Ala Pro Ala Pro Pro Gln Val Leu Pro Gln Ala Pro Ala Pro Ala Pro
            660                 665                 670         
Ala Pro Ala Met Val Ser Ala Leu Ala Gln Ala Pro Ala Pro Val Pro
        675                 680                 685             
Val Leu Ala Pro Gly Pro Pro Gln Ala Val Ala Pro Pro Ala Pro Lys
    690                 695                 700                 
Pro Thr Gln Ala Gly Glu Gly Thr Leu Ser Glu Ala Leu Leu Gln Leu
705                 710                 715                 720 
Gln Phe Asp Asp Glu Asp Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp
                725                 730                 735     
Pro Ala Val Phe Thr Asp Leu Ala Ser Val Asp Asn Ser Glu Phe Gln
            740                 745                 750         
Gln Leu Leu Asn Gln Gly Ile Pro Val Ala Pro His Thr Thr Glu Pro
        755                 760                 765             
Met Leu Met Glu Tyr Pro Glu Ala Ile Thr Arg Leu Val Thr Gly Ala
    770                 775                 780                 
Gln Arg Pro Pro Asp Pro Ala Pro Ala Pro Leu Gly Ala Pro Gly Leu
785                 790                 795                 800 
Pro Asn Gly Leu Leu Ser Gly Asp Glu Asp Phe Ser Ser Ile Ala Asp
                805                 810                 815     
Met Asp Phe Ser Ala Leu Leu Ser Gln Ile Ser Ser
            820                 825             


<210> 41
<211> 119
<212> DNA
<213> Artificial Sequence

<220> 
<223> 7xHBD/mEF1-alpha

<400> 41
tagttaataa tctacaatag ttaataatct acaatagtta ataatctaca atagttaata 60
atctacaata gttaataatc tacaatagtt aataatctac aatagttaat aatctacaa  119

<210> 42
<211> 2586
<212> DNA
<213> Artificial Sequence

<220> 
<223> TamR-tf (HEA4)

<400> 42
atggtgtcca agctgtccca gctgcagaca gaactgctgg cagcactgct ggaaagcggc 60
ctgagcaaag aggccctgat tcaggcactc ggcgaacctg gaccttatct gctcgctggc 120
gaaggccctc tggataaggg cgagagctgt ggcggaggaa gaggagagct ggccgagctg 180
cctaacggcc tgggcgagac aagaggcagc gaggacgaga cagacgacga cggcgaggac 240
ttcacccccc ccatcctgaa agagctggaa aacctgagcc ccgaggaagc cgcccaccag 300
aaagccgtgg tggagacact gctgcaggaa gatccctggc gggtcgccaa gatggtcaag 360
agctacctgc agcagcacaa catcccccag cgggaggtgg tggacaccac cggcctgaac 420
cagagccacc tgagccagca cctgaacaag ggcaccccca tgaaaaccca gaagagagcc 480
gccctgtaca cttggtacgt gcggaagcag agagaggtgg cccagcagtt tacacacgcc 540
ggccagggcg gcctgatcga ggaacctacc ggcgacgagc tgcccaccaa gaagggcaga 600
cggaaccggt ttaagtgggg ccctgcatct cagcagatcc tgttccaggc ctacgagcgg 660
cagaagaacc ccagcaaaga ggaacgggag acactggtgg aagagtgcaa ccgggccgag 720
tgcatccaga gaggcgtgag cccttctcag gctcagggcc tcggcagcaa tctggtcacc 780
gaagtgcggg tgtacaattg gttcgccaac cggcggaaag aggaagcctt ccggcacaag 840
ctgtctgctg gcgatatgag agccgccaac ctgtggccca gccccctgat gatcaagcgg 900
agcaagaaga acagcctggc cctgagcctg accgccgatc agatggtgtc cgctctgctg 960
gacgccgagc cccctatcct gtacagcgag tacgacccca ccagaccctt cagcgaggcc 1020
agcatgatgg gcctgctgac caacctggcc gaccgggagc tggtgcacat gatcaactgg 1080
gccaagcggg tgcccggctt cgtggacctg accctgcacg accaggtcca cctgctggaa 1140
tgtgcctggc tggaaatcct gatgatcggc ctcgtgtgga gaagcatgga acaccccgtg 1200
aagctgctgt tcgcccccaa cctgctcctg gaccggaacc agggaaagtg cgtggagggc 1260
atggtggaga tcttcgacat gctgctggcc acctccagcc ggttccggat gatgaacctg 1320
cagggcgagg aattcgtgtg cctgaagtcc atcatcctgc tgaacagcgg cgtgtacacc 1380
ttcctgtcat ccaccctgaa gtccctggaa gagaaggacc acatccaccg ggtgctggac 1440
aagatcaccg acaccctgat ccacctgatg gccaaggctg gcctgacact ccagcagcag 1500
caccagagac tggcccagct gctgctgatc ctgagccaca tccggcacat gagcaacaag 1560
ggaatggaac acctgtacag catgaagtgc acgaacgtgg tgcccctgta cgacctgctg 1620
ctcgaggctg ccgatgccca cagactgcac gcccctacaa gcagaggcgg agccagcgtg 1680
gaggaaaccg accagtctca cctggccacc gccggcagca caagcagcca cagcctgcag 1740
aagtactaca tcaccggcga ggccgaggga ttccctgcca ccgtggagtt ccagtacctg 1800
cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta cgagacattc 1860
aagagcatca tgaagaagtc ccccttcagc ggccccaccg atcccagacc cccccctaga 1920
agaatcgccg tgcccagcag atctagcgcc agcgtgccca agcctgcccc ccagccctac 1980
cctttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat ggtgttcccc 2040
agcggccaga tctctcaggc ctctgctctg gcacctgctc cacctcaggt gctgcctcag 2100
gcccctgctc cagccccagc ccctgccatg gtgtctgcac tggcccaggc tccagctcct 2160
gtgcctgtgc tggcccctgg acctcctcag gctgtggccc ctcctgcccc taaacctacc 2220
caggccgggg agggaacact gtctgaggcc ctgctgcagc tccagttcga cgacgaggat 2280
ctgggagcac tgctgggcaa tagcaccgac cccgccgtgt ttaccgacct ggcctccgtg 2340
gacaacagcg agttccagca gctcctcaac cagggcatcc ctgtcgcccc acacaccacc 2400
gagcccatgc tgatggaata ccccgaggcc atcaccagac tggtcacagg cgcccagagg 2460
cctccagatc cagcaccagc tccactggga gcccctggcc tgcctaatgg gctgctgtct 2520
ggcgacgagg acttctccag cattgccgac atggacttca gcgccctgct gtcccagatc 2580
agcagc                                                            2586

<210> 43
<211> 862
<212> PRT
<213> Artificial Sequence

<220> 
<223> TamR-tf (HEA4)

<400> 43
Met Val Ser Lys Leu Ser Gln Leu Gln Thr Glu Leu Leu Ala Ala Leu
 1               5                  10                  15      
Leu Glu Ser Gly Leu Ser Lys Glu Ala Leu Ile Gln Ala Leu Gly Glu
            20                  25                  30          
Pro Gly Pro Tyr Leu Leu Ala Gly Glu Gly Pro Leu Asp Lys Gly Glu
        35                  40                  45              
Ser Cys Gly Gly Gly Arg Gly Glu Leu Ala Glu Leu Pro Asn Gly Leu
    50                  55                  60                  
Gly Glu Thr Arg Gly Ser Glu Asp Glu Thr Asp Asp Asp Gly Glu Asp
65                  70                  75                  80  
Phe Thr Pro Pro Ile Leu Lys Glu Leu Glu Asn Leu Ser Pro Glu Glu
                85                  90                  95      
Ala Ala His Gln Lys Ala Val Val Glu Thr Leu Leu Gln Glu Asp Pro
            100                 105                 110         
Trp Arg Val Ala Lys Met Val Lys Ser Tyr Leu Gln Gln His Asn Ile
        115                 120                 125             
Pro Gln Arg Glu Val Val Asp Thr Thr Gly Leu Asn Gln Ser His Leu
    130                 135                 140                 
Ser Gln His Leu Asn Lys Gly Thr Pro Met Lys Thr Gln Lys Arg Ala
145                 150                 155                 160 
Ala Leu Tyr Thr Trp Tyr Val Arg Lys Gln Arg Glu Val Ala Gln Gln
                165                 170                 175     
Phe Thr His Ala Gly Gln Gly Gly Leu Ile Glu Glu Pro Thr Gly Asp
            180                 185                 190         
Glu Leu Pro Thr Lys Lys Gly Arg Arg Asn Arg Phe Lys Trp Gly Pro
        195                 200                 205             
Ala Ser Gln Gln Ile Leu Phe Gln Ala Tyr Glu Arg Gln Lys Asn Pro
    210                 215                 220                 
Ser Lys Glu Glu Arg Glu Thr Leu Val Glu Glu Cys Asn Arg Ala Glu
225                 230                 235                 240 
Cys Ile Gln Arg Gly Val Ser Pro Ser Gln Ala Gln Gly Leu Gly Ser
                245                 250                 255     
Asn Leu Val Thr Glu Val Arg Val Tyr Asn Trp Phe Ala Asn Arg Arg
            260                 265                 270         
Lys Glu Glu Ala Phe Arg His Lys Leu Ser Ala Gly Asp Met Arg Ala
        275                 280                 285             
Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn
    290                 295                 300                 
Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu
305                 310                 315                 320 
Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro
                325                 330                 335     
Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg
            340                 345                 350         
Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val
        355                 360                 365             
Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu
    370                 375                 380                 
Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Val
385                 390                 395                 400 
Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys
                405                 410                 415     
Cys Val Glu Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser
            420                 425                 430         
Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu
        435                 440                 445             
Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser
    450                 455                 460                 
Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp
465                 470                 475                 480 
Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr
                485                 490                 495     
Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser
            500                 505                 510         
His Ile Arg His Met Ser Asn Lys Gly Met Glu His Leu Tyr Ser Met
        515                 520                 525             
Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Ala Ala
    530                 535                 540                 
Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val
545                 550                 555                 560 
Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser Ser
                565                 570                 575     
His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe Pro
            580                 585                 590         
Ala Thr Val Glu Phe Gln Tyr Leu Pro Asp Thr Asp Asp Arg His Arg
        595                 600                 605             
Ile Glu Glu Lys Arg Lys Arg Thr Tyr Glu Thr Phe Lys Ser Ile Met
    610                 615                 620                 
Lys Lys Ser Pro Phe Ser Gly Pro Thr Asp Pro Arg Pro Pro Pro Arg
625                 630                 635                 640 
Arg Ile Ala Val Pro Ser Arg Ser Ser Ala Ser Val Pro Lys Pro Ala
                645                 650                 655     
Pro Gln Pro Tyr Pro Phe Thr Ser Ser Leu Ser Thr Ile Asn Tyr Asp
            660                 665                 670         
Glu Phe Pro Thr Met Val Phe Pro Ser Gly Gln Ile Ser Gln Ala Ser
        675                 680                 685             
Ala Leu Ala Pro Ala Pro Pro Gln Val Leu Pro Gln Ala Pro Ala Pro
    690                 695                 700                 
Ala Pro Ala Pro Ala Met Val Ser Ala Leu Ala Gln Ala Pro Ala Pro
705                 710                 715                 720 
Val Pro Val Leu Ala Pro Gly Pro Pro Gln Ala Val Ala Pro Pro Ala
                725                 730                 735     
Pro Lys Pro Thr Gln Ala Gly Glu Gly Thr Leu Ser Glu Ala Leu Leu
            740                 745                 750         
Gln Leu Gln Phe Asp Asp Glu Asp Leu Gly Ala Leu Leu Gly Asn Ser
        755                 760                 765             
Thr Asp Pro Ala Val Phe Thr Asp Leu Ala Ser Val Asp Asn Ser Glu
    770                 775                 780                 
Phe Gln Gln Leu Leu Asn Gln Gly Ile Pro Val Ala Pro His Thr Thr
785                 790                 795                 800 
Glu Pro Met Leu Met Glu Tyr Pro Glu Ala Ile Thr Arg Leu Val Thr
                805                 810                 815     
Gly Ala Gln Arg Pro Pro Asp Pro Ala Pro Ala Pro Leu Gly Ala Pro
            820                 825                 830         
Gly Leu Pro Asn Gly Leu Leu Ser Gly Asp Glu Asp Phe Ser Ser Ile
        835                 840                 845             
Ala Asp Met Asp Phe Ser Ala Leu Leu Ser Gln Ile Ser Ser
    850                 855                 860         


<210> 44
<211> 408
<212> DNA
<213> Artificial Sequence

<220> 
<223> Her2t

<400> 44
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccatgcc accctgagtg tcagccccag aatggctcag tgacctgttt tggaccggag 120
gctgaccagt gtgtggcctg tgcccactat aaggaccctc ccttctgcgt ggcccgctgc 180
cccagcggtg tgaaacctga cctctcctac atgcccatct ggaagtttcc agatgaggag 240
ggcgcatgcc agccttgccc catcaactgc acccactcct gtgtggacct ggatgacaag 300
ggctgccccg ccgagcagag agccagccct ctgacgtcca tcatctctgc ggtggttggc 360
attctgctgg tcgtggtctt gggggtggtc tttgggatcc tcatctga              408

<210> 45
<211> 135
<212> PRT
<213> Artificial Sequence

<220> 
<223> Her2t

<400> 45
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Cys His Pro Glu Cys Gln Pro Gln Asn Gly
            20                  25                  30          
Ser Val Thr Cys Phe Gly Pro Glu Ala Asp Gln Cys Val Ala Cys Ala
        35                  40                  45              
His Tyr Lys Asp Pro Pro Phe Cys Val Ala Arg Cys Pro Ser Gly Val
    50                  55                  60                  
Lys Pro Asp Leu Ser Tyr Met Pro Ile Trp Lys Phe Pro Asp Glu Glu
65                  70                  75                  80  
Gly Ala Cys Gln Pro Cys Pro Ile Asn Cys Thr His Ser Cys Val Asp
                85                  90                  95      
Leu Asp Asp Lys Gly Cys Pro Ala Glu Gln Arg Ala Ser Pro Leu Thr
            100                 105                 110         
Ser Ile Ile Ser Ala Val Val Gly Ile Leu Leu Val Val Val Leu Gly
        115                 120                 125             
Val Val Phe Gly Ile Leu Ile
    130                 135 


<210> 46
<211> 561
<212> DNA
<213> Artificial Sequence

<220> 
<223> DHFRdm

<400> 46
atggttggtt cgctaaactg catcgtcgct gtgtcccaga acatgggcat cggcaagaac 60
ggggacttcc cctggccacc gctcaggaat gaatccagat atttccagag aatgaccaca 120
acctcttcag tagaaggtaa acagaatctg gtgattatgg gtaagaagac ctggttctcc 180
attcctgaga agaatcgacc tttaaagggt agaattaatt tagttctcag cagagaactc 240
aaggaacctc cacaaggagc tcattttctt tccagaagtc tagatgatgc cttaaaactt 300
actgaacaac cagaattagc aaataaagta gacatggtct ggatagttgg tggcagttct 360
gtttataagg aagccatgaa tcacccaggc catcttaaac tatttgtgac aaggatcatg 420
caagactttg aaagtgacac gttttttcca gaaattgatt tggagaaata taaacttctg 480
ccagaatacc caggtgttct ctctgatgtc caggaggaga aaggcattaa gtacaaattt 540
gaagtatatg agaagaatga t                                           561

<210> 47
<211> 187
<212> PRT
<213> Artificial Sequence

<220> 
<223> DHFRdm

<400> 47
Met Val Gly Ser Leu Asn Cys Ile Val Ala Val Ser Gln Asn Met Gly
 1               5                  10                  15      
Ile Gly Lys Asn Gly Asp Phe Pro Trp Pro Pro Leu Arg Asn Glu Ser
            20                  25                  30          
Arg Tyr Phe Gln Arg Met Thr Thr Thr Ser Ser Val Glu Gly Lys Gln
        35                  40                  45              
Asn Leu Val Ile Met Gly Lys Lys Thr Trp Phe Ser Ile Pro Glu Lys
    50                  55                  60                  
Asn Arg Pro Leu Lys Gly Arg Ile Asn Leu Val Leu Ser Arg Glu Leu
65                  70                  75                  80  
Lys Glu Pro Pro Gln Gly Ala His Phe Leu Ser Arg Ser Leu Asp Asp
                85                  90                  95      
Ala Leu Lys Leu Thr Glu Gln Pro Glu Leu Ala Asn Lys Val Asp Met
            100                 105                 110         
Val Trp Ile Val Gly Gly Ser Ser Val Tyr Lys Glu Ala Met Asn His
        115                 120                 125             
Pro Gly His Leu Lys Leu Phe Val Thr Arg Ile Met Gln Asp Phe Glu
    130                 135                 140                 
Ser Asp Thr Phe Phe Pro Glu Ile Asp Leu Glu Lys Tyr Lys Leu Leu
145                 150                 155                 160 
Pro Glu Tyr Pro Gly Val Leu Ser Asp Val Gln Glu Glu Lys Gly Ile
                165                 170                 175     
Lys Tyr Lys Phe Glu Val Tyr Glu Lys Asn Asp
            180                 185         


<210> 48

<400> 48
 000

<210> 49

<400> 49
 000

<210> 50

<400> 50
 000

<210> 51

<400> 51
 000

<210> 52

<400> 52
 000

<210> 53

<400> 53
 000

<210> 54

<400> 54
 000

<210> 55

<400> 55
 000

<210> 56

<400> 56
 000

<210> 57

<400> 57
 000

<210> 58

<400> 58
 000

<210> 59
<211> 98
<212> PRT
<213> Artificial Sequence

<220> 
<223> CH3 domain

<400> 59
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg
 1               5                  10                  15      
Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr
65                  70                  75                  80  
Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys
                85                  90                  95      
Arg Val
        


<210> 60
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Hinge domain

<400> 60
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro
 1               5                  10          


<210> 61
<211> 110
<212> PRT
<213> Artificial Sequence

<220> 
<223> CH2 domain

<400> 61
Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
 1               5                  10                  15      
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
            20                  25                  30          
Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr
        35                  40                  45              
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
    50                  55                  60                  
Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
65                  70                  75                  80  
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
                85                  90                  95      
Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
            100                 105                 110 


<210> 62
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> CH3 protein domain

<400> 62
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
 1               5                  10                  15      
Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
            20                  25                  30          
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
        35                  40                  45              
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
    50                  55                  60                  
Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
65                  70                  75                  80  
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
                85                  90                  95      
Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
            100                 105         


<210> 63

<400> 63
 000

<210> 64
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Signal peptide

<400> 64
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro
 1               5                  10          


<210> 65
<211> 134
<212> PRT
<213> Artificial Sequence

<220> 
<223> Extracellular domain

<400> 65
Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn
 1               5                  10                  15      
Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu
            20                  25                  30          
Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys
        35                  40                  45              
Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr
    50                  55                  60                  
Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr
65                  70                  75                  80  
Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile
                85                  90                  95      
Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly
            100                 105                 110         
Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe
        115                 120                 125             
Pro Gly Pro Ser Lys Pro
    130                 


<210> 66
<211> 27
<212> PRT
<213> Artificial Sequence

<220> 
<223> Transmembrane domain

<400> 66
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
            20                  25          


<210> 67
<211> 50
<212> PRT
<213> Artificial Sequence

<220> 
<223> Intracellular domain

<400> 67
Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu
 1               5                  10                  15      
Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr
            20                  25                  30          
Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr
        35                  40                  45              
Arg Ser
    50  


<210> 68

<220> 
<223> Signal peptide

<400> 68
 000

<210> 69
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> Signal peptide

<400> 69
Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu
 1               5                  10                  15      
Asn Phe Glu Arg Thr Arg Ser
            20              


<210> 70
<211> 166
<212> PRT
<213> Artificial Sequence

<220> 
<223> Extracellular domain

<400> 70
Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro Ala Gly Thr Phe
 1               5                  10                  15      
Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys Pro Pro Asn Ser
            20                  25                  30          
Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile Cys Arg Gln Cys
        35                  40                  45              
Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser Thr Ser Asn Ala
    50                  55                  60                  
Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly Ala Gly Cys Ser
65                  70                  75                  80  
Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu Thr Lys Lys Gly
                85                  90                  95      
Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln Lys Arg Gly Ile
            100                 105                 110         
Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys Ser Val Leu Val
        115                 120                 125             
Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro Ser Pro Ala Asp
    130                 135                 140                 
Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala Pro Ala Arg Glu
145                 150                 155                 160 
Pro Gly His Ser Pro Gln
                165     


<210> 71
<211> 27
<212> PRT
<213> Artificial Sequence

<220> 
<223> Transmembrane domain

<400> 71
Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu
 1               5                  10                  15      
Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val
            20                  25          


<210> 72
<211> 42
<212> PRT
<213> Artificial Sequence

<220> 
<223> Intracellular domain

<400> 72
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
 1               5                  10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
        35                  40          


<210> 73
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> Signal peptide

<400> 73
Met Lys Trp Lys Ala Leu Phe Thr Ala Ala Ile Leu Gln Ala Gln Leu
 1               5                  10                  15      
Pro Ile Thr Glu Ala
            20      


<210> 74
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Extracellular domain

<400> 74
Gln Ser Phe Gly Leu Leu Asp Pro Lys
 1               5                  


<210> 75
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> Transmembrane domain

<400> 75
Leu Cys Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu
 1               5                  10                  15      
Thr Ala Leu Phe Leu
            20      


<210> 76
<211> 113
<212> PRT
<213> Artificial Sequence

<220> 
<223> Intracellular domain

<400> 76
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln
    50                  55                  60                  
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu
65                  70                  75                  80  
Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr
                85                  90                  95      
Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro
            100                 105                 110         
Arg
    


<210> 77
<211> 29
<212> PRT
<213> Artificial Sequence

<220> 
<223> ITAM I

<400> 77
Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn
 1               5                  10                  15      
Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg
            20                  25                  


<210> 78
<211> 29
<212> PRT
<213> Artificial Sequence

<220> 
<223> ITAM 2

<400> 78
Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln
 1               5                  10                  15      
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met
            20                  25                  


<210> 79
<211> 29
<212> PRT
<213> Artificial Sequence

<220> 
<223> ITAM 3

<400> 79
Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser
 1               5                  10                  15      
Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln
            20                  25                  


<210> 80
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> Leader

<400> 80
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atccca                                                            66

<210> 81
<211> 734
<212> DNA
<213> Artificial Sequence

<220> 
<223> Her2scFV

<400> 81
gatatccaga tgacccagtc cccgagctcc ctgtccgcct ctgtgggcga tagggtcacc 60
atcacctgcc gtgccagtca ggatgtgaat actgctgtag cctggtatca acagaaacca 120
ggaaaagctc cgaaactact gatttactcg gcatccttcc tctactctgg agtcccttct 180
cgcttctctg gttccagatc tgggacggat ttcactctga ccatcagcag tctgcagccg 240
gaagacttcg caacttatta ctgtcagcaa cattatacta ctcctcccac gttcggacag 300
ggtaccaagg tggagatcaa aggcagtact agcggcggtg gctccggggg cggatccggt 360
gggggcggca gcagcgaggt tcagctggtg gagtctggcg gtggcctggt gcagccaggg 420
ggctcactcc gtttgtcctg tgcagcttct ggcttcaaca ttaaagacac ctatatacac 480
tgggtgcgtc aggccccggg taagggcctg gaatgggttg caaggattta tcctacgaat 540
ggttatacta gatatgccga tagcgtcaag ggccgtttca ctataagcgc agacacatcc 600
aaaaacacag cctacctgca gatgaacagc ctgcgtgctg aggacactgc cgtctattat 660
tgttctagat ggggagggga cggcttctat gctatggact actggggtca aggaaccctg 720
gtcaccgtct cgag                                                   734

<210> 82
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Hinge spacer

<400> 82
gagagcaagt acggaccgcc ctgcccccct tgccct                           36

<210> 83
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD28tm

<400> 83
atgttctggg tgctggtggt ggtcggaggc gtgctggcct gctacagcct gctggtcacc 60
gtggccttca tcatcttttg ggtg                                        84

<210> 84
<211> 126
<212> DNA
<213> Artificial Sequence

<220> 
<223> 4-1BB

<400> 84
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa 60
actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt 120
gaactg                                                            126

<210> 85
<211> 336
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD3 zeta

<400> 85
cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca gaatcagctg 60
tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa gcggagaggc 120
cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg cctgtataac 180
gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg 240
aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac caaggatacc 300
tacgacgccc tgcacatgca ggccctgccc ccaagg                           336

<210> 86
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A

<400> 86
ctcgagggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 60
cccggcccta gg                                                     72

<210> 87
<211> 1074
<212> DNA
<213> Artificial Sequence

<220> 
<223> tEGFR

<400> 87
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata 120
aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc 180
ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa 240
ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 300
gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag 360
caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc 420
tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat 480
gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata 540
agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 600
cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga 660
ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag 720
aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc 780
acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc 840
gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 900
gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca 960
ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg 1020
ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gtga       1074

<210> 88
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized ScFv comprising a variable light chain
      comprising a CDRL1 sequence

<400> 88
Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn
 1               5                  10      


<210> 89
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDRL2

<400> 89
Ser Arg Leu His Ser Gly Val
 1               5          


<210> 90
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDRL3

<400> 90
Gly Asn Thr Leu Pro Tyr Thr Phe Gly
 1               5                  


<210> 91
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDRH1

<400> 91
Asp Tyr Gly Val Ser
 1               5  


<210> 92
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDRH2

<400> 92
Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser
 1               5                  10                  15      


<210> 93
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDRH3

<400> 93
Tyr Ala Met Asp Tyr Trp Gly
 1               5          


<210> 94
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> Flexible linker between the VH and VL chains in
      the scFv

<400> 94
Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr
 1               5                  10                  15      
Lys Gly
        

