                         SEQUENCE LISTING

<110>  Moralco B.V.
 
<120>  Use of acetaldehyde in the fermentative production of ethanol

<130>  P6050975PCT

<150>  EP14165291.7
<151>  2014-04-18

<150>  EP14171443.6
<151>  2014-06-06

<150>  EP14194989.1
<151>  2014-11-26

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  993
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  1
gatttttata atgacgaaaa aaaaaaaatt ggaaagaaaa agcttaatgc ggtagtttat     60

cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg    120

tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc    180

cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc    240

tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc    300

gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga    360

tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca    420

ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc    480

gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg    540

tggccggggg actgttgggc gccatctcct tgcatgcaac ttcttttctt tttttttctt    600

ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca    660

ctaaaggaaa aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat    720

gaggggtatc tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct    780

aatgagcaac ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt    840

gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat    900

tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag    960

catacaatca aggaattcga gctaagcggc cgc                                 993


<210>  2
<211>  816
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  2
ccatatgatc atgtgtcgtc gcacacatat atatatgcct gtatgtgtca gcactaaagt     60

tgcctggcca tccacgctat atatacacgc ctggcggatc tgctcgagga ttgcctacgc    120

gtgggcttga tccaccaacc aacgctcgcc aaatgaactg gcgctttggt cttctgccat    180

cgtccgtaaa ccccggccaa agagaccgga aagatcggtg aaaacatctt gatcttgctc    240

ccgggaattt tagattcagg taggaaattg attacatcaa tactgttacc ctgaatcata    300

ttcgacgatg tcgtctcaca cggaaatata attcatttct tggttttcca aaaaaatttt    360

catttttttt cacttttttg tttcgtcctc cttttttttt tttttttttt attttttttc    420

ctgtgttcac cttttttttt ttcagttgac atctttctgc attcttttct gtgttttttt    480

tttttttttt cgtttttcca ttgttcgttc gttgcctgtt ttttcgccct attgttctcg    540

agcctaaaaa ttttttcctt tcctgctttc ctttcttcgt tcaaagtttc ctattccatt    600

gttctctttg gtaaactcat tgttgtcgga actcagatat attcaggtca atttactgta    660

cttcaattga cttttttctt gaaatttcaa cttgcctttt caacttgttc ttctttttta    720

atcttattct acactttagt tcccttacct tgttcctaat tattgtctag caaaaagaaa    780

acatacacct atttcattca cacactgcag aaaatg                              816


<210>  3
<211>  500
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<223>  S. cerevisiae ALD6 (Accession NO. NP_015264)

<400>  3

Met Thr Lys Leu His Phe Asp Thr Ala Glu Pro Val Lys Ile Thr Leu 
1               5                   10                  15      


Pro Asn Gly Leu Thr Tyr Glu Gln Pro Thr Gly Leu Phe Ile Asn Asn 
            20                  25                  30          


Lys Phe Met Lys Ala Gln Asp Gly Lys Thr Tyr Pro Val Glu Asp Pro 
        35                  40                  45              


Ser Thr Glu Asn Thr Val Cys Glu Val Ser Ser Ala Thr Thr Glu Asp 
    50                  55                  60                  


Val Glu Tyr Ala Ile Glu Cys Ala Asp Arg Ala Phe His Asp Thr Glu 
65                  70                  75                  80  


Trp Ala Thr Gln Asp Pro Arg Glu Arg Gly Arg Leu Leu Ser Lys Leu 
                85                  90                  95      


Ala Asp Glu Leu Glu Ser Gln Ile Asp Leu Val Ser Ser Ile Glu Ala 
            100                 105                 110         


Leu Asp Asn Gly Lys Thr Leu Ala Leu Ala Arg Gly Asp Val Thr Ile 
        115                 120                 125             


Ala Ile Asn Cys Leu Arg Asp Ala Ala Ala Tyr Ala Asp Lys Val Asn 
    130                 135                 140                 


Gly Arg Thr Ile Asn Thr Gly Asp Gly Tyr Met Asn Phe Thr Thr Leu 
145                 150                 155                 160 


Glu Pro Ile Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro Ile 
                165                 170                 175     


Met Met Leu Ala Trp Lys Ile Ala Pro Ala Leu Ala Met Gly Asn Val 
            180                 185                 190         


Cys Ile Leu Lys Pro Ala Ala Val Thr Pro Leu Asn Ala Leu Tyr Phe 
        195                 200                 205             


Ala Ser Leu Cys Lys Lys Val Gly Ile Pro Ala Gly Val Val Asn Ile 
    210                 215                 220                 


Val Pro Gly Pro Gly Arg Thr Val Gly Ala Ala Leu Thr Asn Asp Pro 
225                 230                 235                 240 


Arg Ile Arg Lys Leu Ala Phe Thr Gly Ser Thr Glu Val Gly Lys Ser 
                245                 250                 255     


Val Ala Val Asp Ser Ser Glu Ser Asn Leu Lys Lys Ile Thr Leu Glu 
            260                 265                 270         


Leu Gly Gly Lys Ser Ala His Leu Val Phe Asp Asp Ala Asn Ile Lys 
        275                 280                 285             


Lys Thr Leu Pro Asn Leu Val Asn Gly Ile Phe Lys Asn Ala Gly Gln 
    290                 295                 300                 


Ile Cys Ser Ser Gly Ser Arg Ile Tyr Val Gln Glu Gly Ile Tyr Asp 
305                 310                 315                 320 


Glu Leu Leu Ala Ala Phe Lys Ala Tyr Leu Glu Thr Glu Ile Lys Val 
                325                 330                 335     


Gly Asn Pro Phe Asp Lys Ala Asn Phe Gln Gly Ala Ile Thr Asn Arg 
            340                 345                 350         


Gln Gln Phe Asp Thr Ile Met Asn Tyr Ile Asp Ile Gly Lys Lys Glu 
        355                 360                 365             


Gly Ala Lys Ile Leu Thr Gly Gly Glu Lys Val Gly Asp Lys Gly Tyr 
    370                 375                 380                 


Phe Ile Arg Pro Thr Val Phe Tyr Asp Val Asn Glu Asp Met Arg Ile 
385                 390                 395                 400 


Val Lys Glu Glu Ile Phe Gly Pro Val Val Thr Val Ala Lys Phe Lys 
                405                 410                 415     


Thr Leu Glu Glu Gly Val Glu Met Ala Asn Ser Ser Glu Phe Gly Leu 
            420                 425                 430         


Gly Ser Gly Ile Glu Thr Glu Ser Leu Ser Thr Gly Leu Lys Val Ala 
        435                 440                 445             


Lys Met Leu Lys Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asp Phe 
    450                 455                 460                 


Asp Ser Arg Val Pro Phe Gly Gly Val Lys Gln Ser Gly Tyr Gly Arg 
465                 470                 475                 480 


Glu Met Gly Glu Glu Val Tyr His Ala Tyr Thr Glu Val Lys Ala Val 
                485                 490                 495     


Arg Ile Lys Leu 
            500 


<210>  4
<211>  391
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  4

Met Ser Ala Ala Ala Asp Arg Leu Asn Leu Thr Ser Gly His Leu Asn 
1               5                   10                  15      


Ala Gly Arg Lys Arg Ser Ser Ser Ser Val Ser Leu Lys Ala Ala Glu 
            20                  25                  30          


Lys Pro Phe Lys Val Thr Val Ile Gly Ser Gly Asn Trp Gly Thr Thr 
        35                  40                  45              


Ile Ala Lys Val Val Ala Glu Asn Cys Lys Gly Tyr Pro Glu Val Phe 
    50                  55                  60                  


Ala Pro Ile Val Gln Met Trp Val Phe Glu Glu Glu Ile Asn Gly Glu 
65                  70                  75                  80  


Lys Leu Thr Glu Ile Ile Asn Thr Arg His Gln Asn Val Lys Tyr Leu 
                85                  90                  95      


Pro Gly Ile Thr Leu Pro Asp Asn Leu Val Ala Asn Pro Asp Leu Ile 
            100                 105                 110         


Asp Ser Val Lys Asp Val Asp Ile Ile Val Phe Asn Ile Pro His Gln 
        115                 120                 125             


Phe Leu Pro Arg Ile Cys Ser Gln Leu Lys Gly His Val Asp Ser His 
    130                 135                 140                 


Val Arg Ala Ile Ser Cys Leu Lys Gly Phe Glu Val Gly Ala Lys Gly 
145                 150                 155                 160 


Val Gln Leu Leu Ser Ser Tyr Ile Thr Glu Glu Leu Gly Ile Gln Cys 
                165                 170                 175     


Gly Ala Leu Ser Gly Ala Asn Ile Ala Thr Glu Val Ala Gln Glu His 
            180                 185                 190         


Trp Ser Glu Thr Thr Val Ala Tyr His Ile Pro Lys Asp Phe Arg Gly 
        195                 200                 205             


Glu Gly Lys Asp Val Asp His Lys Val Leu Lys Ala Leu Phe His Arg 
    210                 215                 220                 


Pro Tyr Phe His Val Ser Val Ile Glu Asp Val Ala Gly Ile Ser Ile 
225                 230                 235                 240 


Cys Gly Ala Leu Lys Asn Val Val Ala Leu Gly Cys Gly Phe Val Glu 
                245                 250                 255     


Gly Leu Gly Trp Gly Asn Asn Ala Ser Ala Ala Ile Gln Arg Val Gly 
            260                 265                 270         


Leu Gly Glu Ile Ile Arg Phe Gly Gln Met Phe Phe Pro Glu Ser Arg 
        275                 280                 285             


Glu Glu Thr Tyr Tyr Gln Glu Ser Ala Gly Val Ala Asp Leu Ile Thr 
    290                 295                 300                 


Thr Cys Ala Gly Gly Arg Asn Val Lys Val Ala Arg Leu Met Ala Thr 
305                 310                 315                 320 


Ser Gly Lys Asp Ala Trp Glu Cys Glu Lys Glu Leu Leu Asn Gly Gln 
                325                 330                 335     


Ser Ala Gln Gly Leu Ile Thr Cys Lys Glu Val His Glu Trp Leu Glu 
            340                 345                 350         


Thr Cys Gly Ser Val Glu Asp Phe Pro Leu Phe Glu Ala Val Tyr Gln 
        355                 360                 365             


Ile Val Tyr Asn Asn Tyr Pro Met Lys Asn Leu Pro Asp Met Ile Glu 
    370                 375                 380                 


Glu Leu Asp Leu His Glu Asp 
385                 390     


<210>  5
<211>  440
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  5

Met Leu Ala Val Arg Arg Leu Thr Arg Tyr Thr Phe Leu Lys Arg Thr 
1               5                   10                  15      


His Pro Val Leu Tyr Thr Arg Arg Ala Tyr Lys Ile Leu Pro Ser Arg 
            20                  25                  30          


Ser Thr Phe Leu Arg Arg Ser Leu Leu Gln Thr Gln Leu His Ser Lys 
        35                  40                  45              


Met Thr Ala His Thr Asn Ile Lys Gln His Lys His Cys His Glu Asp 
    50                  55                  60                  


His Pro Ile Arg Arg Ser Asp Ser Ala Val Ser Ile Val His Leu Lys 
65                  70                  75                  80  


Arg Ala Pro Phe Lys Val Thr Val Ile Gly Ser Gly Asn Trp Gly Thr 
                85                  90                  95      


Thr Ile Ala Lys Val Ile Ala Glu Asn Thr Glu Leu His Ser His Ile 
            100                 105                 110         


Phe Glu Pro Glu Val Arg Met Trp Val Phe Asp Glu Lys Ile Gly Asp 
        115                 120                 125             


Glu Asn Leu Thr Asp Ile Ile Asn Thr Arg His Gln Asn Val Lys Tyr 
    130                 135                 140                 


Leu Pro Asn Ile Asp Leu Pro His Asn Leu Val Ala Asp Pro Asp Leu 
145                 150                 155                 160 


Leu His Ser Ile Lys Gly Ala Asp Ile Leu Val Phe Asn Ile Pro His 
                165                 170                 175     


Gln Phe Leu Pro Asn Ile Val Lys Gln Leu Gln Gly His Val Ala Pro 
            180                 185                 190         


His Val Arg Ala Ile Ser Cys Leu Lys Gly Phe Glu Leu Gly Ser Lys 
        195                 200                 205             


Gly Val Gln Leu Leu Ser Ser Tyr Val Thr Asp Glu Leu Gly Ile Gln 
    210                 215                 220                 


Cys Gly Ala Leu Ser Gly Ala Asn Leu Ala Pro Glu Val Ala Lys Glu 
225                 230                 235                 240 


His Trp Ser Glu Thr Thr Val Ala Tyr Gln Leu Pro Lys Asp Tyr Gln 
                245                 250                 255     


Gly Asp Gly Lys Asp Val Asp His Lys Ile Leu Lys Leu Leu Phe His 
            260                 265                 270         


Arg Pro Tyr Phe His Val Asn Val Ile Asp Asp Val Ala Gly Ile Ser 
        275                 280                 285             


Ile Ala Gly Ala Leu Lys Asn Val Val Ala Leu Ala Cys Gly Phe Val 
    290                 295                 300                 


Glu Gly Met Gly Trp Gly Asn Asn Ala Ser Ala Ala Ile Gln Arg Leu 
305                 310                 315                 320 


Gly Leu Gly Glu Ile Ile Lys Phe Gly Arg Met Phe Phe Pro Glu Ser 
                325                 330                 335     


Lys Val Glu Thr Tyr Tyr Gln Glu Ser Ala Gly Val Ala Asp Leu Ile 
            340                 345                 350         


Thr Thr Cys Ser Gly Gly Arg Asn Val Lys Val Ala Thr Tyr Met Ala 
        355                 360                 365             


Lys Thr Gly Lys Ser Ala Leu Glu Ala Glu Lys Glu Leu Leu Asn Gly 
    370                 375                 380                 


Gln Ser Ala Gln Gly Ile Ile Thr Cys Arg Glu Val His Glu Trp Leu 
385                 390                 395                 400 


Gln Thr Cys Glu Leu Thr Gln Glu Phe Pro Leu Phe Glu Ala Val Tyr 
                405                 410                 415     


Gln Ile Val Tyr Asn Asn Val Arg Met Glu Asp Leu Pro Glu Met Ile 
            420                 425                 430         


Glu Glu Leu Asp Ile Asp Asp Glu 
        435                 440 


<210>  6
<211>  250
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  6

Met Gly Leu Thr Thr Lys Pro Leu Ser Leu Lys Val Asn Ala Ala Leu 
1               5                   10                  15      


Phe Asp Val Asp Gly Thr Ile Ile Ile Ser Gln Pro Ala Ile Ala Ala 
            20                  25                  30          


Phe Trp Arg Asp Phe Gly Lys Asp Lys Pro Tyr Phe Asp Ala Glu His 
        35                  40                  45              


Val Ile Gln Val Ser His Gly Trp Arg Thr Phe Asp Ala Ile Ala Lys 
    50                  55                  60                  


Phe Ala Pro Asp Phe Ala Asn Glu Glu Tyr Val Asn Lys Leu Glu Ala 
65                  70                  75                  80  


Glu Ile Pro Val Lys Tyr Gly Glu Lys Ser Ile Glu Val Pro Gly Ala 
                85                  90                  95      


Val Lys Leu Cys Asn Ala Leu Asn Ala Leu Pro Lys Glu Lys Trp Ala 
            100                 105                 110         


Val Ala Thr Ser Gly Thr Arg Asp Met Ala Gln Lys Trp Phe Glu His 
        115                 120                 125             


Leu Gly Ile Arg Arg Pro Lys Tyr Phe Ile Thr Ala Asn Asp Val Lys 
    130                 135                 140                 


Gln Gly Lys Pro His Pro Glu Pro Tyr Leu Lys Gly Arg Asn Gly Leu 
145                 150                 155                 160 


Gly Tyr Pro Ile Asn Glu Gln Asp Pro Ser Lys Ser Lys Val Val Val 
                165                 170                 175     


Phe Glu Asp Ala Pro Ala Gly Ile Ala Ala Gly Lys Ala Ala Gly Cys 
            180                 185                 190         


Lys Ile Ile Gly Ile Ala Thr Thr Phe Asp Leu Asp Phe Leu Lys Glu 
        195                 200                 205             


Lys Gly Cys Asp Ile Ile Val Lys Asn His Glu Ser Ile Arg Val Gly 
    210                 215                 220                 


Gly Tyr Asn Ala Glu Thr Asp Glu Val Glu Phe Ile Phe Asp Asp Tyr 
225                 230                 235                 240 


Leu Tyr Ala Lys Asp Asp Leu Leu Lys Trp 
                245                 250 


<210>  7
<211>  250
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  7

Met Pro Leu Thr Thr Lys Pro Leu Ser Leu Lys Ile Asn Ala Ala Leu 
1               5                   10                  15      


Phe Asp Val Asp Gly Thr Ile Ile Ile Ser Gln Pro Ala Ile Ala Ala 
            20                  25                  30          


Phe Trp Arg Asp Phe Gly Lys Asp Lys Pro Tyr Phe Asp Ala Glu His 
        35                  40                  45              


Val Ile His Ile Ser His Gly Trp Arg Thr Tyr Asp Ala Ile Ala Lys 
    50                  55                  60                  


Phe Ala Pro Asp Phe Ala Asp Glu Glu Tyr Val Asn Lys Leu Glu Gly 
65                  70                  75                  80  


Glu Ile Pro Glu Lys Tyr Gly Glu His Ser Ile Glu Val Pro Gly Ala 
                85                  90                  95      


Val Lys Leu Cys Asn Ala Leu Asn Ala Leu Pro Lys Glu Lys Trp Ala 
            100                 105                 110         


Val Ala Thr Ser Gly Thr Arg Asp Met Ala Lys Lys Trp Phe Asp Ile 
        115                 120                 125             


Leu Lys Ile Lys Arg Pro Glu Tyr Phe Ile Thr Ala Asn Asp Val Lys 
    130                 135                 140                 


Gln Gly Lys Pro His Pro Glu Pro Tyr Leu Lys Gly Arg Asn Gly Leu 
145                 150                 155                 160 


Gly Phe Pro Ile Asn Glu Gln Asp Pro Ser Lys Ser Lys Val Val Val 
                165                 170                 175     


Phe Glu Asp Ala Pro Ala Gly Ile Ala Ala Gly Lys Ala Ala Gly Cys 
            180                 185                 190         


Lys Ile Val Gly Ile Ala Thr Thr Phe Asp Leu Asp Phe Leu Lys Glu 
        195                 200                 205             


Lys Gly Cys Asp Ile Ile Val Lys Asn His Glu Ser Ile Arg Val Gly 
    210                 215                 220                 


Glu Tyr Asn Ala Glu Thr Asp Glu Val Glu Leu Ile Phe Asp Asp Tyr 
225                 230                 235                 240 


Leu Tyr Ala Lys Asp Asp Leu Leu Lys Trp 
                245                 250 


<210>  8
<211>  669
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<223>  S. cerevisiae FPS1 (Accession No. NP_013057)

<400>  8

Met Ser Asn Pro Gln Lys Ala Leu Asn Asp Phe Leu Ser Ser Glu Ser 
1               5                   10                  15      


Val His Thr His Asp Ser Ser Arg Lys Gln Ser Asn Lys Gln Ser Ser 
            20                  25                  30          


Asp Glu Gly Arg Ser Ser Ser Gln Pro Ser His His His Ser Gly Gly 
        35                  40                  45              


Thr Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Ser Asn Asn 
    50                  55                  60                  


Asn Asn Asn Gly Asn Asp Gly Gly Asn Asp Asp Asp Tyr Asp Tyr Glu 
65                  70                  75                  80  


Met Gln Asp Tyr Arg Pro Ser Pro Gln Ser Ala Arg Pro Thr Pro Thr 
                85                  90                  95      


Tyr Val Pro Gln Tyr Ser Val Glu Ser Gly Thr Ala Phe Pro Ile Gln 
            100                 105                 110         


Glu Val Ile Pro Ser Ala Tyr Ile Asn Thr Gln Asp Ile Asn His Lys 
        115                 120                 125             


Asp Asn Gly Pro Pro Ser Ala Ser Ser Asn Arg Ala Phe Arg Pro Arg 
    130                 135                 140                 


Gly Gln Thr Thr Val Ser Ala Asn Val Leu Asn Ile Glu Asp Phe Tyr 
145                 150                 155                 160 


Lys Asn Ala Asp Asp Ala His Thr Ile Pro Glu Ser His Leu Ser Arg 
                165                 170                 175     


Arg Arg Ser Arg Ser Arg Ala Thr Ser Asn Ala Gly His Ser Ala Asn 
            180                 185                 190         


Thr Gly Ala Thr Asn Gly Arg Thr Thr Gly Ala Gln Thr Asn Met Glu 
        195                 200                 205             


Ser Asn Glu Ser Pro Arg Asn Val Pro Ile Met Val Lys Pro Lys Thr 
    210                 215                 220                 


Leu Tyr Gln Asn Pro Gln Thr Pro Thr Val Leu Pro Ser Thr Tyr His 
225                 230                 235                 240 


Pro Ile Asn Lys Trp Ser Ser Val Lys Asn Thr Tyr Leu Lys Glu Phe 
                245                 250                 255     


Leu Ala Glu Phe Met Gly Thr Met Val Met Ile Ile Phe Gly Ser Ala 
            260                 265                 270         


Val Val Cys Gln Val Asn Val Ala Gly Lys Ile Gln Gln Asp Asn Phe 
        275                 280                 285             


Asn Val Ala Leu Asp Asn Leu Asn Val Thr Gly Ser Ser Ala Glu Thr 
    290                 295                 300                 


Ile Asp Ala Met Lys Ser Leu Thr Ser Leu Val Ser Ser Val Ala Gly 
305                 310                 315                 320 


Gly Thr Phe Asp Asp Val Ala Leu Gly Trp Ala Ala Ala Val Val Met 
                325                 330                 335     


Gly Tyr Phe Cys Ala Gly Gly Ser Ala Ile Ser Gly Ala His Leu Asn 
            340                 345                 350         


Pro Ser Ile Thr Leu Ala Asn Leu Val Tyr Arg Gly Phe Pro Leu Lys 
        355                 360                 365             


Lys Val Pro Tyr Tyr Phe Ala Gly Gln Leu Ile Gly Ala Phe Thr Gly 
    370                 375                 380                 


Ala Leu Ile Leu Phe Ile Trp Tyr Lys Arg Val Leu Gln Glu Ala Tyr 
385                 390                 395                 400 


Ser Asp Trp Trp Met Asn Glu Ser Val Ala Gly Met Phe Cys Val Phe 
                405                 410                 415     


Pro Lys Pro Tyr Leu Ser Ser Gly Arg Gln Phe Phe Ser Glu Phe Leu 
            420                 425                 430         


Cys Gly Ala Met Leu Gln Ala Gly Thr Phe Ala Leu Thr Asp Pro Tyr 
        435                 440                 445             


Thr Cys Leu Ser Ser Asp Val Phe Pro Leu Met Met Phe Ile Leu Ile 
    450                 455                 460                 


Phe Ile Ile Asn Ala Ser Met Ala Tyr Gln Thr Gly Thr Ala Met Asn 
465                 470                 475                 480 


Leu Ala Arg Asp Leu Gly Pro Arg Leu Ala Leu Tyr Ala Val Gly Phe 
                485                 490                 495     


Asp His Lys Met Leu Trp Val His His His His Phe Phe Trp Val Pro 
            500                 505                 510         


Met Val Gly Pro Phe Ile Gly Ala Leu Met Gly Gly Leu Val Tyr Asp 
        515                 520                 525             


Val Cys Ile Tyr Gln Gly His Glu Ser Pro Val Asn Trp Ser Leu Pro 
    530                 535                 540                 


Val Tyr Lys Glu Met Ile Met Arg Ala Trp Phe Arg Arg Pro Gly Trp 
545                 550                 555                 560 


Lys Lys Arg Asn Arg Ala Arg Arg Thr Ser Asp Leu Ser Asp Phe Ser 
                565                 570                 575     


Tyr Asn Asn Asp Asp Asp Glu Glu Phe Gly Glu Arg Met Ala Leu Gln 
            580                 585                 590         


Lys Thr Lys Thr Lys Ser Ser Ile Ser Asp Asn Glu Asn Glu Ala Gly 
        595                 600                 605             


Glu Lys Lys Val Gln Phe Lys Ser Val Gln Arg Gly Lys Arg Thr Phe 
    610                 615                 620                 


Gly Gly Ile Pro Thr Ile Leu Glu Glu Glu Asp Ser Ile Glu Thr Ala 
625                 630                 635                 640 


Ser Leu Gly Ala Thr Thr Thr Asp Ser Ile Gly Leu Ser Asp Thr Ser 
                645                 650                 655     


Ser Glu Asp Ser His Tyr Gly Asn Ala Lys Lys Val Thr 
            660                 665                 


<210>  9
<211>  348
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<223>  S. cerevisiae ADH1 (Accession No. NP_014555)

<400>  9

Met Ser Ile Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Ser His 
1               5                   10                  15      


Gly Lys Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Ala Asn 
            20                  25                  30          


Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu 
        35                  40                  45              


His Ala Trp His Gly Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val 
    50                  55                  60                  


Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val 
65                  70                  75                  80  


Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly 
                85                  90                  95      


Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys 
            100                 105                 110         


Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln 
        115                 120                 125             


Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr 
    130                 135                 140                 


Asp Leu Ala Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr 
145                 150                 155                 160 


Lys Ala Leu Lys Ser Ala Asn Leu Met Ala Gly His Trp Val Ala Ile 
                165                 170                 175     


Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys 
            180                 185                 190         


Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Glu Gly Lys Glu 
        195                 200                 205             


Glu Leu Phe Arg Ser Ile Gly Gly Glu Val Phe Ile Asp Phe Thr Lys 
    210                 215                 220                 


Glu Lys Asp Ile Val Gly Ala Val Leu Lys Ala Thr Asp Gly Gly Ala 
225                 230                 235                 240 


His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser 
                245                 250                 255     


Thr Arg Tyr Val Arg Ala Asn Gly Thr Thr Val Leu Val Gly Met Pro 
            260                 265                 270         


Ala Gly Ala Lys Cys Cys Ser Asp Val Phe Asn Gln Val Val Lys Ser 
        275                 280                 285             


Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu 
    290                 295                 300                 


Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val 
305                 310                 315                 320 


Val Gly Leu Ser Thr Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly 
                325                 330                 335     


Gln Ile Val Gly Arg Tyr Val Val Asp Thr Ser Lys 
            340                 345             


<210>  10
<211>  386
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  10

Met Ser Ala Ala Thr Val Gly Lys Pro Ile Lys Cys Ile Ala Ala Val 
1               5                   10                  15      


Ala Tyr Asp Ala Lys Lys Pro Leu Ser Val Glu Glu Ile Thr Val Asp 
            20                  25                  30          


Ala Pro Lys Ala His Glu Val Arg Ile Lys Ile Glu Tyr Thr Ala Val 
        35                  40                  45              


Cys His Thr Asp Ala Tyr Thr Leu Ser Gly Ser Asp Pro Glu Gly Leu 
    50                  55                  60                  


Phe Pro Cys Val Leu Gly His Glu Gly Ala Gly Ile Val Glu Ser Val 
65                  70                  75                  80  


Gly Asp Asp Val Ile Thr Val Lys Pro Gly Asp His Val Ile Ala Leu 
                85                  90                  95      


Tyr Thr Ala Glu Cys Gly Lys Cys Lys Phe Cys Thr Ser Gly Lys Thr 
            100                 105                 110         


Asn Leu Cys Gly Ala Val Arg Ala Thr Gln Gly Lys Gly Val Met Pro 
        115                 120                 125             


Asp Gly Thr Thr Arg Phe His Asn Ala Lys Gly Glu Asp Ile Tyr His 
    130                 135                 140                 


Phe Met Gly Cys Ser Thr Phe Ser Glu Tyr Thr Val Val Ala Asp Val 
145                 150                 155                 160 


Ser Val Val Ala Ile Asp Pro Lys Ala Pro Leu Asp Ala Ala Cys Leu 
                165                 170                 175     


Leu Gly Cys Gly Val Thr Thr Gly Phe Gly Ala Ala Leu Lys Thr Ala 
            180                 185                 190         


Asn Val Gln Lys Gly Asp Thr Val Ala Val Phe Gly Cys Gly Thr Val 
        195                 200                 205             


Gly Leu Ser Val Ile Gln Gly Ala Lys Leu Arg Gly Ala Ser Lys Ile 
    210                 215                 220                 


Ile Ala Ile Asp Ile Asn Asn Lys Lys Lys Gln Tyr Cys Ser Gln Phe 
225                 230                 235                 240 


Gly Ala Thr Asp Phe Val Asn Pro Lys Glu Asp Leu Ala Lys Asp Gln 
                245                 250                 255     


Thr Ile Val Glu Lys Leu Ile Glu Met Thr Asp Gly Gly Leu Asp Phe 
            260                 265                 270         


Thr Phe Asp Cys Thr Gly Asn Thr Lys Ile Met Arg Asp Ala Leu Glu 
        275                 280                 285             


Ala Cys His Lys Gly Trp Gly Gln Ser Ile Ile Ile Gly Val Ala Ala 
    290                 295                 300                 


Ala Gly Glu Glu Ile Ser Thr Arg Pro Phe Gln Leu Val Thr Gly Arg 
305                 310                 315                 320 


Val Trp Lys Gly Ser Ala Phe Gly Gly Ile Lys Gly Arg Ser Glu Met 
                325                 330                 335     


Gly Gly Leu Ile Lys Asp Tyr Gln Lys Gly Ala Leu Lys Val Glu Glu 
            340                 345                 350         


Phe Ile Thr His Arg Arg Pro Phe Lys Glu Ile Asn Gln Ala Phe Glu 
        355                 360                 365             


Asp Leu His Asn Gly Asp Cys Leu Arg Thr Val Leu Lys Ser Asp Glu 
    370                 375                 380                 


Ile Lys 
385     


<210>  11
<211>  678
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  11

Met Gly Leu Leu Ala Leu Gly Thr Pro Leu Gln Trp Phe Glu Ser Arg 
1               5                   10                  15      


Thr Tyr Asn Glu His Ile Arg Asp Glu Gly Ile Glu Gln Leu Leu Tyr 
            20                  25                  30          


Ile Phe Gln Ala Ala Gly Lys Arg Asp Asn Asp Pro Leu Phe Trp Gly 
        35                  40                  45              


Asp Glu Leu Glu Tyr Met Val Val Asp Phe Asp Asp Lys Glu Arg Asn 
    50                  55                  60                  


Ser Met Leu Asp Val Cys His Asp Lys Ile Leu Thr Glu Leu Asn Met 
65                  70                  75                  80  


Glu Asp Ser Ser Leu Cys Glu Ala Asn Asp Val Ser Phe His Pro Glu 
                85                  90                  95      


Tyr Gly Arg Tyr Met Leu Glu Ala Thr Pro Ala Ser Pro Tyr Leu Asn 
            100                 105                 110         


Tyr Val Gly Ser Tyr Val Glu Val Asn Met Gln Lys Arg Arg Ala Ile 
        115                 120                 125             


Ala Glu Tyr Lys Leu Ser Glu Tyr Ala Arg Gln Asp Ser Lys Asn Asn 
    130                 135                 140                 


Leu His Val Gly Ser Arg Ser Val Pro Leu Thr Leu Thr Val Phe Pro 
145                 150                 155                 160 


Arg Met Gly Cys Pro Asp Phe Ile Asn Ile Lys Asp Pro Trp Asn His 
                165                 170                 175     


Lys Asn Ala Ala Ser Arg Ser Leu Phe Leu Pro Asp Glu Val Ile Asn 
            180                 185                 190         


Arg His Val Arg Phe Pro Asn Leu Thr Ala Ser Ile Arg Thr Arg Arg 
        195                 200                 205             


Gly Glu Lys Val Cys Met Asn Val Pro Met Tyr Lys Asp Ile Ala Thr 
    210                 215                 220                 


Pro Glu Thr Asp Asp Ser Ile Tyr Asp Arg Asp Trp Phe Leu Pro Glu 
225                 230                 235                 240 


Asp Lys Glu Ala Lys Leu Ala Ser Lys Pro Gly Phe Ile Tyr Met Asp 
                245                 250                 255     


Ser Met Gly Phe Gly Met Gly Cys Ser Cys Leu Gln Val Thr Phe Gln 
            260                 265                 270         


Ala Pro Asn Ile Asn Lys Ala Arg Tyr Leu Tyr Asp Ala Leu Val Asn 
        275                 280                 285             


Phe Ala Pro Ile Met Leu Ala Phe Ser Ala Ala Ala Pro Ala Phe Lys 
    290                 295                 300                 


Gly Trp Leu Ala Asp Gln Asp Val Arg Trp Asn Val Ile Ser Gly Ala 
305                 310                 315                 320 


Val Asp Asp Arg Thr Pro Lys Glu Arg Gly Val Ala Pro Leu Leu Pro 
                325                 330                 335     


Lys Tyr Asn Lys Asn Gly Phe Gly Gly Ile Ala Lys Asp Val Gln Asp 
            340                 345                 350         


Lys Val Leu Glu Ile Pro Lys Ser Arg Tyr Ser Ser Val Asp Leu Phe 
        355                 360                 365             


Leu Gly Gly Ser Lys Phe Phe Asn Arg Thr Tyr Asn Asp Thr Asn Val 
    370                 375                 380                 


Pro Ile Asn Glu Lys Val Leu Gly Arg Leu Leu Glu Asn Asp Lys Ala 
385                 390                 395                 400 


Pro Leu Asp Tyr Asp Leu Ala Lys His Phe Ala His Leu Tyr Ile Arg 
                405                 410                 415     


Asp Pro Val Ser Thr Phe Glu Glu Leu Leu Asn Gln Asp Asn Lys Thr 
            420                 425                 430         


Ser Ser Asn His Phe Glu Asn Ile Gln Ser Thr Asn Trp Gln Thr Leu 
        435                 440                 445             


Arg Phe Lys Pro Pro Thr Gln Gln Ala Thr Pro Asp Lys Lys Asp Ser 
    450                 455                 460                 


Pro Gly Trp Arg Val Glu Phe Arg Pro Phe Glu Val Gln Leu Leu Asp 
465                 470                 475                 480 


Phe Glu Asn Ala Ala Tyr Ser Val Leu Ile Tyr Leu Ile Val Asp Ser 
                485                 490                 495     


Ile Leu Thr Phe Ser Asp Asn Ile Asn Ala Tyr Ile His Met Ser Lys 
            500                 505                 510         


Val Trp Glu Asn Met Lys Ile Ala His His Arg Asp Ala Ile Leu Phe 
        515                 520                 525             


Glu Lys Phe His Trp Lys Lys Ser Phe Arg Asn Asp Thr Asp Val Glu 
    530                 535                 540                 


Thr Glu Asp Tyr Ser Ile Ser Glu Ile Phe His Asn Pro Glu Asn Gly 
545                 550                 555                 560 


Ile Phe Pro Gln Phe Val Thr Pro Ile Leu Cys Gln Lys Gly Phe Val 
                565                 570                 575     


Thr Lys Asp Trp Lys Glu Leu Lys His Ser Ser Lys His Glu Arg Leu 
            580                 585                 590         


Tyr Tyr Tyr Leu Lys Leu Ile Ser Asp Arg Ala Ser Gly Glu Leu Pro 
        595                 600                 605             


Thr Thr Ala Lys Phe Phe Arg Asn Phe Val Leu Gln His Pro Asp Tyr 
    610                 615                 620                 


Lys His Asp Ser Lys Ile Ser Lys Ser Ile Asn Tyr Asp Leu Leu Ser 
625                 630                 635                 640 


Thr Cys Asp Arg Leu Thr His Leu Asp Asp Ser Lys Gly Glu Leu Thr 
                645                 650                 655     


Ser Phe Leu Gly Ala Glu Ile Ala Glu Tyr Val Lys Lys Asn Lys Pro 
            660                 665                 670         


Ser Ile Glu Ser Lys Cys 
        675             


<210>  12
<211>  428
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  12

Met Thr Ala Ala Lys Pro Asn Pro Tyr Ala Ala Lys Pro Gly Asp Tyr 
1               5                   10                  15      


Leu Ser Asn Val Asn Asn Phe Gln Leu Ile Asp Ser Thr Leu Arg Glu 
            20                  25                  30          


Gly Glu Gln Phe Ala Asn Ala Phe Phe Asp Thr Glu Lys Lys Ile Glu 
        35                  40                  45              


Ile Ala Arg Ala Leu Asp Asp Phe Gly Val Asp Tyr Ile Glu Leu Thr 
    50                  55                  60                  


Ser Pro Val Ala Ser Glu Gln Ser Arg Lys Asp Cys Glu Ala Ile Cys 
65                  70                  75                  80  


Lys Leu Gly Leu Lys Ala Lys Ile Leu Thr His Ile Arg Cys His Met 
                85                  90                  95      


Asp Asp Ala Lys Val Ala Val Glu Thr Gly Val Asp Gly Val Asp Val 
            100                 105                 110         


Val Ile Gly Thr Ser Lys Phe Leu Arg Gln Tyr Ser His Gly Lys Asp 
        115                 120                 125             


Met Asn Tyr Ile Ala Lys Ser Ala Val Glu Val Ile Glu Phe Val Lys 
    130                 135                 140                 


Ser Lys Gly Ile Glu Ile Arg Phe Ser Ser Glu Asp Ser Phe Arg Ser 
145                 150                 155                 160 


Asp Leu Val Asp Leu Leu Asn Ile Tyr Lys Thr Val Asp Lys Ile Gly 
                165                 170                 175     


Val Asn Arg Val Gly Ile Ala Asp Thr Val Gly Cys Ala Asn Pro Arg 
            180                 185                 190         


Gln Val Tyr Glu Leu Ile Arg Thr Leu Lys Ser Val Val Ser Cys Asp 
        195                 200                 205             


Ile Glu Cys His Phe His Asn Asp Thr Gly Cys Ala Ile Ala Asn Ala 
    210                 215                 220                 


Tyr Thr Ala Leu Glu Gly Gly Ala Arg Leu Ile Asp Val Ser Val Leu 
225                 230                 235                 240 


Gly Ile Gly Glu Arg Asn Gly Ile Thr Pro Leu Gly Gly Leu Met Ala 
                245                 250                 255     


Arg Met Ile Val Ala Ala Pro Asp Tyr Val Lys Ser Lys Tyr Lys Leu 
            260                 265                 270         


His Lys Ile Arg Asp Ile Glu Asn Leu Val Ala Asp Ala Val Glu Val 
        275                 280                 285             


Asn Ile Pro Phe Asn Asn Pro Ile Thr Gly Phe Cys Ala Phe Thr His 
    290                 295                 300                 


Lys Ala Gly Ile His Ala Lys Ala Ile Leu Ala Asn Pro Ser Thr Tyr 
305                 310                 315                 320 


Glu Ile Leu Asp Pro His Asp Phe Gly Met Lys Arg Tyr Ile His Phe 
                325                 330                 335     


Ala Asn Arg Leu Thr Gly Trp Asn Ala Ile Lys Ala Arg Val Asp Gln 
            340                 345                 350         


Leu Asn Leu Asn Leu Thr Asp Asp Gln Ile Lys Glu Val Thr Ala Lys 
        355                 360                 365             


Ile Lys Lys Leu Gly Asp Val Arg Ser Leu Asn Ile Asp Asp Val Asp 
    370                 375                 380                 


Ser Ile Ile Lys Asn Phe His Ala Glu Val Ser Thr Pro Gln Val Leu 
385                 390                 395                 400 


Ser Ala Lys Lys Asn Lys Lys Asn Asp Ser Asp Val Pro Glu Leu Ala 
                405                 410                 415     


Thr Ile Pro Ala Ala Lys Arg Thr Lys Pro Ser Ala 
            420                 425             


