                         SEQUENCE LISTING

<110>  UAB Research Foundation
       Niederweis, Michael
       Pavlenok, Mikhail
 
<120>  MSP NANOPORES AND USES THEREOF

<130>  35979-941614 (160WO1)

<150>  US 61/980,415
<151>  2014-04-16

<150>  US 61/980,393
<151>  2014-04-16

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  184
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  1

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala 
                85                  90                  95      


Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Asp Val Ser Gly Ala Glu Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  2
<211>  184
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  2

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala 
                85                  90                  95      


Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  3
<211>  184
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  3

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Gly 
                85                  90                  95      


Pro Pro Phe Gly Leu Glu Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  4
<211>  176
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  4

Val Asp Gly Gln Gly Arg Thr Leu Thr Val Gln Gln Ala Glu Thr Phe 
1               5                   10                  15      


Leu Asn Gly Val Phe Pro Leu Asp Arg Asn Arg Leu Thr Arg Glu Trp 
            20                  25                  30          


Phe His Ser Gly Arg Ala Thr Tyr His Val Ala Gly Pro Gly Ala Asp 
        35                  40                  45              


Glu Phe Glu Gly Thr Leu Glu Leu Gly Tyr Gln Val Gly Phe Pro Trp 
    50                  55                  60                  


Ser Leu Gly Val Gly Ile Asn Phe Ser Tyr Thr Thr Pro Asn Ile Leu 
65                  70                  75                  80  


Ile Asp Gly Gly Asp Ile Thr Gln Pro Pro Phe Gly Leu Asp Thr Ile 
                85                  90                  95      


Ile Thr Pro Asn Leu Phe Pro Gly Val Ser Ile Ser Ala Asp Leu Gly 
            100                 105                 110         


Asn Gly Pro Gly Ile Gln Glu Val Ala Thr Phe Ser Val Asp Val Lys 
        115                 120                 125             


Gly Ala Lys Gly Ala Val Ala Val Ser Asn Ala His Gly Thr Val Thr 
    130                 135                 140                 


Gly Ala Ala Gly Gly Val Leu Leu Arg Pro Phe Ala Arg Leu Ile Ala 
145                 150                 155                 160 


Ser Thr Gly Asp Ser Val Thr Thr Tyr Gly Glu Pro Trp Asn Met Asn 
                165                 170                 175     


<210>  5
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic construct

<400>  5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  6
<211>  211
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  6

Met Lys Ala Ile Ser Arg Val Leu Ile Ala Met Val Ala Ala Ile Ala 
1               5                   10                  15      


Ala Leu Phe Thr Ser Thr Gly Thr Ser His Ala Gly Leu Asp Asn Glu 
            20                  25                  30          


Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu Thr Val Gln Gln Trp 
        35                  40                  45              


Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg Asn Arg Leu Thr 
    50                  55                  60                  


Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr Ile Val Ala Gly Pro 
65                  70                  75                  80  


Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly Tyr Gln Ile Gly 
                85                  90                  95      


Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser Tyr Thr Thr Pro 
            100                 105                 110         


Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala Pro Pro Phe Gly Leu 
        115                 120                 125             


Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly Val Ser Ile Ser Ala 
    130                 135                 140                 


Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala Thr Phe Ser Val 
145                 150                 155                 160 


Asp Val Ser Gly Ala Glu Gly Gly Val Ala Val Ser Asn Ala His Gly 
                165                 170                 175     


Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg Pro Phe Ala Arg 
            180                 185                 190         


Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr Gly Glu Pro Trp 
        195                 200                 205             


Asn Met Asn 
    210     


<210>  7
<211>  215
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  7

Met Thr Ala Phe Lys Arg Val Leu Ile Ala Met Ile Ser Ala Leu Leu 
1               5                   10                  15      


Ala Gly Thr Thr Gly Met Phe Val Ser Ala Gly Ala Ala His Ala Gly 
            20                  25                  30          


Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu Thr 
        35                  40                  45              


Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg 
    50                  55                  60                  


Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr Ile 
65                  70                  75                  80  


Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly 
                85                  90                  95      


Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser 
            100                 105                 110         


Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala Pro 
        115                 120                 125             


Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly Val 
    130                 135                 140                 


Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala 
145                 150                 155                 160 


Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val Ser 
                165                 170                 175     


Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg 
            180                 185                 190         


Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr 
        195                 200                 205             


Gly Glu Pro Trp Asn Met Asn 
    210                 215 


<210>  8
<211>  215
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  8

Met Lys Ala Ile Ser Arg Val Leu Ile Ala Met Ile Ser Ala Leu Ala 
1               5                   10                  15      


Ala Ala Val Ala Gly Leu Phe Val Ser Ala Gly Thr Ser His Ala Gly 
            20                  25                  30          


Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu Thr 
        35                  40                  45              


Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg 
    50                  55                  60                  


Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr Ile 
65                  70                  75                  80  


Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly 
                85                  90                  95      


Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser 
            100                 105                 110         


Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Gly Pro 
        115                 120                 125             


Pro Phe Gly Leu Glu Ser Val Ile Thr Pro Asn Leu Phe Pro Gly Val 
    130                 135                 140                 


Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala 
145                 150                 155                 160 


Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val Ser 
                165                 170                 175     


Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg 
            180                 185                 190         


Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr 
        195                 200                 205             


Gly Glu Pro Trp Asn Met Asn 
    210                 215 


<210>  9
<211>  207
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  9

Met Arg Tyr Leu Val Met Met Phe Ala Leu Leu Val Ser Val Thr Leu 
1               5                   10                  15      


Val Ser Pro Arg Pro Ala Asn Ala Val Asp Asn Gln Leu Ser Val Val 
            20                  25                  30          


Asp Gly Gln Gly Arg Thr Leu Thr Val Gln Gln Ala Glu Thr Phe Leu 
        35                  40                  45              


Asn Gly Val Phe Pro Leu Asp Arg Asn Arg Leu Thr Arg Glu Trp Phe 
    50                  55                  60                  


His Ser Gly Arg Ala Thr Tyr His Val Ala Gly Pro Gly Ala Asp Glu 
65                  70                  75                  80  


Phe Glu Gly Thr Leu Glu Leu Gly Tyr Gln Val Gly Phe Pro Trp Ser 
                85                  90                  95      


Leu Gly Val Gly Ile Asn Phe Ser Tyr Thr Thr Pro Asn Ile Leu Ile 
            100                 105                 110         


Asp Gly Gly Asp Ile Thr Gln Pro Pro Phe Gly Leu Asp Thr Ile Ile 
        115                 120                 125             


Thr Pro Asn Leu Phe Pro Gly Val Ser Ile Ser Ala Asp Leu Gly Asn 
    130                 135                 140                 


Gly Pro Gly Ile Gln Glu Val Ala Thr Phe Ser Val Asp Val Lys Gly 
145                 150                 155                 160 


Ala Lys Gly Ala Val Ala Val Ser Asn Ala His Gly Thr Val Thr Gly 
                165                 170                 175     


Ala Ala Gly Gly Val Leu Leu Arg Pro Phe Ala Arg Leu Ile Ala Ser 
            180                 185                 190         


Thr Gly Asp Ser Val Thr Thr Tyr Gly Glu Pro Trp Asn Met Asn 
        195                 200                 205         


<210>  10
<211>  549
<212>  DNA
<213>  Mycobacterium smegmatis

<400>  10
ggcctggaca acgagctgag cctcgttgat ggccaggacc gcaccctcac cgtgcagcag       60

tgggacacct tcctcaatgg tgtgttcccc ctggaccgca accgtcttac ccgtgagtgg      120

ttccactccg gtcgcgccaa gtacatcgtg gccggccccg gtgccgacga gttcgagggc      180

acgctggaac tcggctacca gatcggcttc ccgtggtcgc tgggtgtggg catcaacttc      240

agctacacca ccccgaacat cctgatcgac gacggtgaca tcaccgctcc gccgttcggc      300

ctgaactcgg tcatcacccc gaacctgttc cccggtgtgt cgatctcggc agatctgggc      360

aacggccccg gcatccagga agtcgcaacg ttctcggtcg acgtctccgg cgccgagggt      420

ggcgtggccg tgtcgaacgc ccacggcacc gtgaccggtg cggccggcgg tgtgctgctg      480

cgtccgttcg cccgcctgat cgcctcgacc ggtgactcgg tcaccaccta cggcgaaccc      540

tggaacatg                                                              549


