                         SEQUENCE LISTING

<110>  Conagen Inc.
       Han, Jixiang
       Chen, Hui
       Yu, Oliver
 
<120>  METHOD FOR PRODUCING ERGOTHIONEINE

<130>  32559-11

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1287
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1
atgatcgcac gcgagacact ggccgacgag ctggccctgg cccgcgaacg cacgttgcgg       60

ctcgtggagt tcgacgacgc ggaactgcat cgccagtaca acccgctgat gagcccgctc      120

gtgtgggacc tcgcgcacat cgggcagcag gaagaactgt ggctgctgcg cgacggcaac      180

cccgaccgcc ccggcatgct cgcacccgag gtggaccggc tttacgacgc gttcgagcac      240

tcacgcgcca gccgggtcaa cctcccgttg ctgccgcctt cggatgcgcg cgcctactgc      300

gcgacggtgc gggccaaggc gctcgacacc ctcgacacgc tgcccgagga cgatccgggc      360

ttccggttcg cgctggtgat cagccacgag aaccagcacg acgagaccat gctgcaggca      420

ctcaacctgc gcgagggccc acccctgctc gacaccggaa ttcccctgcc cgcgggcagg      480

ccaggcgtgg caggcacgtc ggtgctggtg ccgggcggcc cgttcgtgct cggggtcgac      540

gcgctgaccg aaccgcactc actggacaac gaacggcccg cccacgtcgt ggacatcccg      600

tcgttccgga tcggccgcgt gccggtcacc aacgccgaat ggcgcgagtt catcgacgac      660

ggtggctacg accaaccgcg ctggtggtcg ccacgcggct gggcgcaccg ccaggaggcg      720

ggcctggtgg ccccgcagtt ctggaacccc gacggcaccc gcacccggtt cgggcacatc      780

gaggagatcc cgggtgacga acccgtgcag cacgtgacgt tcttcgaagc cgaggcctac      840

gcggcgtggg ccggtgctcg gttgcccacc gagatcgaat gggagaaggc ctgcgcgtgg      900

gatccggtcg ccggtgctcg gcgccggttc ccctggggct cagcacaacc cagcgcggcg      960

ctggccaacc tcggcggtga cgcacgccgc ccggcgccgg tcggggccta cccggcgggg     1020

gcgtcggcct atggcgccga gcagatgctg ggcgacgtgt gggagtggac ctcctcgccg     1080

ctgcggccgt ggcccggttt cacgccgatg atctacgagc gctacagcac gccgttcttc     1140

gagggcacca catccggtga ctaccgcgtg ctgcgcggcg ggtcatgggc cgttgcaccg     1200

ggaatcctgc ggcccagctt ccgcaactgg gaccacccga tccggcggca gatattctcg     1260

ggtgtccgcc tggcctggga cgtctga                                         1287


<210>  2
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2

Met Ile Ala Arg Glu Thr Leu Ala Asp Glu Leu Ala Leu Ala Arg Glu 
1               5                   10                  15      


Arg Thr Leu Arg Leu Val Glu Phe Asp Asp Ala Glu Leu His Arg Gln 
            20                  25                  30          


Tyr Asn Pro Leu Met Ser Pro Leu Val Trp Asp Leu Ala His Ile Gly 
        35                  40                  45              


Gln Gln Glu Glu Leu Trp Leu Leu Arg Asp Gly Asn Pro Asp Arg Pro 
    50                  55                  60                  


Gly Met Leu Ala Pro Glu Val Asp Arg Leu Tyr Asp Ala Phe Glu His 
65                  70                  75                  80  


Ser Arg Ala Ser Arg Val Asn Leu Pro Leu Leu Pro Pro Ser Asp Ala 
                85                  90                  95      


Arg Ala Tyr Cys Ala Thr Val Arg Ala Lys Ala Leu Asp Thr Leu Asp 
            100                 105                 110         


Thr Leu Pro Glu Asp Asp Pro Gly Phe Arg Phe Ala Leu Val Ile Ser 
        115                 120                 125             


His Glu Asn Gln His Asp Glu Thr Met Leu Gln Ala Leu Asn Leu Arg 
    130                 135                 140                 


Glu Gly Pro Pro Leu Leu Asp Thr Gly Ile Pro Leu Pro Ala Gly Arg 
145                 150                 155                 160 


Pro Gly Val Ala Gly Thr Ser Val Leu Val Pro Gly Gly Pro Phe Val 
                165                 170                 175     


Leu Gly Val Asp Ala Leu Thr Glu Pro His Ser Leu Asp Asn Glu Arg 
            180                 185                 190         


Pro Ala His Val Val Asp Ile Pro Ser Phe Arg Ile Gly Arg Val Pro 
        195                 200                 205             


Val Thr Asn Ala Glu Trp Arg Glu Phe Ile Asp Asp Gly Gly Tyr Asp 
    210                 215                 220                 


Gln Pro Arg Trp Trp Ser Pro Arg Gly Trp Ala His Arg Gln Glu Ala 
225                 230                 235                 240 


Gly Leu Val Ala Pro Gln Phe Trp Asn Pro Asp Gly Thr Arg Thr Arg 
                245                 250                 255     


Phe Gly His Ile Glu Glu Ile Pro Gly Asp Glu Pro Val Gln His Val 
            260                 265                 270         


Thr Phe Phe Glu Ala Glu Ala Tyr Ala Ala Trp Ala Gly Ala Arg Leu 
        275                 280                 285             


Pro Thr Glu Ile Glu Trp Glu Lys Ala Cys Ala Trp Asp Pro Val Ala 
    290                 295                 300                 


Gly Ala Arg Arg Arg Phe Pro Trp Gly Ser Ala Gln Pro Ser Ala Ala 
305                 310                 315                 320 


Leu Ala Asn Leu Gly Gly Asp Ala Arg Arg Pro Ala Pro Val Gly Ala 
                325                 330                 335     


Tyr Pro Ala Gly Ala Ser Ala Tyr Gly Ala Glu Gln Met Leu Gly Asp 
            340                 345                 350         


Val Trp Glu Trp Thr Ser Ser Pro Leu Arg Pro Trp Pro Gly Phe Thr 
        355                 360                 365             


Pro Met Ile Tyr Glu Arg Tyr Ser Thr Pro Phe Phe Glu Gly Thr Thr 
    370                 375                 380                 


Ser Gly Asp Tyr Arg Val Leu Arg Gly Gly Ser Trp Ala Val Ala Pro 
385                 390                 395                 400 


Gly Ile Leu Arg Pro Ser Phe Arg Asn Trp Asp His Pro Ile Arg Arg 
                405                 410                 415     


Gln Ile Phe Ser Gly Val Arg Leu Ala Trp Asp Val 
            420                 425             


<210>  3
<211>  684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3
atgtgccggc atgtggcgtg gctgggcgcg ccgcggtcgt tggccgacct ggtgctcgac       60

ccgccgcagg gactgctggt gcagtcctac gcaccgcgac gacagaagca cggtctgatg      120

aacgccgacg gttggggcgc agggtttttc gacgacgagg gagtggcccg ccgctggcgc      180

agcgacaaac cgctgtgggg tgatgcgtcg ttcgcgtcgg tggcacccgc actacgcagt      240

cgttgcgtgc tggccgcggt gcgctcggcc accatcggca tgcccatcga accgtcggcg      300

tcggcgccgt tcagcgacgg gcagtggctg ctgtcgcaca acggcctggt cgaccgcggg      360

gtgctcccgt tgaccggtgc cgccgagtcc acggtggaca gcgcgatcgt cgcggcgctc      420

atcttctccc gtggcctcga cgcgctcggc gccaccatcg ccgaggtcgg cgaactcgac      480

ccgaacgcgc ggttgaacat cctggccgcc aacggttccc ggctgctcgc caccacctgg      540

ggggacacgc tgtcggtcct gcaccgcccc gacggcgtcg tcctcgcgag cgaaccctac      600

gacgacgatc ccggctggtc ggacatcccg gaccggcacc tcgtcgacgt ccgcgacgcc      660

cacgtcgtcg tgacacccct gtga                                             684


<210>  4
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4

Met Cys Arg His Val Ala Trp Leu Gly Ala Pro Arg Ser Leu Ala Asp 
1               5                   10                  15      


Leu Val Leu Asp Pro Pro Gln Gly Leu Leu Val Gln Ser Tyr Ala Pro 
            20                  25                  30          


Arg Arg Gln Lys His Gly Leu Met Asn Ala Asp Gly Trp Gly Ala Gly 
        35                  40                  45              


Phe Phe Asp Asp Glu Gly Val Ala Arg Arg Trp Arg Ser Asp Lys Pro 
    50                  55                  60                  


Leu Trp Gly Asp Ala Ser Phe Ala Ser Val Ala Pro Ala Leu Arg Ser 
65                  70                  75                  80  


Arg Cys Val Leu Ala Ala Val Arg Ser Ala Thr Ile Gly Met Pro Ile 
                85                  90                  95      


Glu Pro Ser Ala Ser Ala Pro Phe Ser Asp Gly Gln Trp Leu Leu Ser 
            100                 105                 110         


His Asn Gly Leu Val Asp Arg Gly Val Leu Pro Leu Thr Gly Ala Ala 
        115                 120                 125             


Glu Ser Thr Val Asp Ser Ala Ile Val Ala Ala Leu Ile Phe Ser Arg 
    130                 135                 140                 


Gly Leu Asp Ala Leu Gly Ala Thr Ile Ala Glu Val Gly Glu Leu Asp 
145                 150                 155                 160 


Pro Asn Ala Arg Leu Asn Ile Leu Ala Ala Asn Gly Ser Arg Leu Leu 
                165                 170                 175     


Ala Thr Thr Trp Gly Asp Thr Leu Ser Val Leu His Arg Pro Asp Gly 
            180                 185                 190         


Val Val Leu Ala Ser Glu Pro Tyr Asp Asp Asp Pro Gly Trp Ser Asp 
        195                 200                 205             


Ile Pro Asp Arg His Leu Val Asp Val Arg Asp Ala His Val Val Val 
    210                 215                 220                 


Thr Pro Leu 
225         


<210>  5
<211>  966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
atgacgctct cactggccaa ctacctggca gccgactcgg ccgccgaagc actgcgccgt       60

gacgtccgcg cgggcctcac cgcggcaccg aagagtctgc cgcccaagtg gttctacgac      120

gccgtcggca gtgatctgtt cgaccagatc acccggctcc ccgagtatta ccccacccgc      180

accgaggcgc agatcctgcg gacccggtcg gcggagatca tcgcggccgc gggtgccgac      240

accctggtgg aactgggcag tggtacgtcg gagaaaaccc gcatgctgct cgacgccatg      300

cgcgacgccg agttgctgcg ccgcttcatc ccgttcgacg tcgacgcggg cgtgctgcgc      360

tcggccgggg cggcaatcgg cgcggagtac cccggtatcg agatcgacgc ggtatgtggc      420

gatttcgagg aacatctggg caagatcccg catgtcggac ggcggctcgt ggtgttcctg      480

gggtcgacca tcggcaacct gacacccgcg ccccgcgcgg agttcctcag tactctcgcg      540

gacacgctgc agccgggcga cagcctgctg ctgggcaccg atctggtgaa ggacaccggc      600

cggttggtgc gcgcgtacga cgacgcggcc ggcgtcaccg cggcgttcaa ccgcaacgtg      660

ctggccgtgg tgaaccgcga actgtccgcc gatttcgacc tcgacgcgtt cgagcatgtc      720

gcgaagtgga actccgacga ggaacgcatc gagatgtggt tgcgtgcccg caccgcacag      780

catgtccgcg tcgcggcact ggacctggag gtcgacttcg ccgcgggtga ggagatgctc      840

accgaggtgt cctgcaagtt ccgtcccgag aacgtcgtcg ccgagctggc ggaagccggt      900

ctgcggcaga cgcattggtg gaccgatccg gccggggatt tcgggttgtc gctggcggtg      960

cggtga                                                                 966


<210>  6
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6

Met Thr Leu Ser Leu Ala Asn Tyr Leu Ala Ala Asp Ser Ala Ala Glu 
1               5                   10                  15      


Ala Leu Arg Arg Asp Val Arg Ala Gly Leu Thr Ala Ala Pro Lys Ser 
            20                  25                  30          


Leu Pro Pro Lys Trp Phe Tyr Asp Ala Val Gly Ser Asp Leu Phe Asp 
        35                  40                  45              


Gln Ile Thr Arg Leu Pro Glu Tyr Tyr Pro Thr Arg Thr Glu Ala Gln 
    50                  55                  60                  


Ile Leu Arg Thr Arg Ser Ala Glu Ile Ile Ala Ala Ala Gly Ala Asp 
65                  70                  75                  80  


Thr Leu Val Glu Leu Gly Ser Gly Thr Ser Glu Lys Thr Arg Met Leu 
                85                  90                  95      


Leu Asp Ala Met Arg Asp Ala Glu Leu Leu Arg Arg Phe Ile Pro Phe 
            100                 105                 110         


Asp Val Asp Ala Gly Val Leu Arg Ser Ala Gly Ala Ala Ile Gly Ala 
        115                 120                 125             


Glu Tyr Pro Gly Ile Glu Ile Asp Ala Val Cys Gly Asp Phe Glu Glu 
    130                 135                 140                 


His Leu Gly Lys Ile Pro His Val Gly Arg Arg Leu Val Val Phe Leu 
145                 150                 155                 160 


Gly Ser Thr Ile Gly Asn Leu Thr Pro Ala Pro Arg Ala Glu Phe Leu 
                165                 170                 175     


Ser Thr Leu Ala Asp Thr Leu Gln Pro Gly Asp Ser Leu Leu Leu Gly 
            180                 185                 190         


Thr Asp Leu Val Lys Asp Thr Gly Arg Leu Val Arg Ala Tyr Asp Asp 
        195                 200                 205             


Ala Ala Gly Val Thr Ala Ala Phe Asn Arg Asn Val Leu Ala Val Val 
    210                 215                 220                 


Asn Arg Glu Leu Ser Ala Asp Phe Asp Leu Asp Ala Phe Glu His Val 
225                 230                 235                 240 


Ala Lys Trp Asn Ser Asp Glu Glu Arg Ile Glu Met Trp Leu Arg Ala 
                245                 250                 255     


Arg Thr Ala Gln His Val Arg Val Ala Ala Leu Asp Leu Glu Val Asp 
            260                 265                 270         


Phe Ala Ala Gly Glu Glu Met Leu Thr Glu Val Ser Cys Lys Phe Arg 
        275                 280                 285             


Pro Glu Asn Val Val Ala Glu Leu Ala Glu Ala Gly Leu Arg Gln Thr 
    290                 295                 300                 


His Trp Trp Thr Asp Pro Ala Gly Asp Phe Gly Leu Ser Leu Ala Val 
305                 310                 315                 320 


Arg 
    


<210>  7
<211>  1113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
atgctcgcgc agcagtggcg tgacgcccgt cccaaggttg ccgggttgca cctggacagc       60

ggggcatgtt cgcggcagag cttcgcggtg atcgacgcga ccaccgcaca cgcacgccac      120

gaggccgagg tgggtggtta tgtggcggcc gaggctgcga cgccggcgct cgacgccggg      180

cgggccgcgg tcgcgtcgct catcggtttt gcggcgtcgg acgtggtgta caccagcgga      240

tccaaccacg ccatcgacct gttgctgtcg agctggccgg ggaagcgcac gctggcctgc      300

ctgcccggcg agtacgggcc gaatctgtct gccatggcgg ccaacggttt ccaggtgcgt      360

gcgctaccgg tcgacgacga cgggcgggtg ctggtcgacg aggcgtcgca cgaactgtcg      420

gcccatcccg tcgcgctcgt acacctcacc gcattggcaa gccatcgcgg gatcgcgcaa      480

cccgcggcag aactcgtcga ggcctgccac aatgcgggga tccccgtggt gatcgacgcc      540

gcgcaggcgc tggggcatct ggactgcaat gtcggggccg acgcggtgta ctcatcgtcg      600

cgcaagtggc tcgccggccc gcgtggtgtc ggggtgctcg cggtgcggcc cgaactcgcc      660

gagcgtctgc aaccgcggat ccccccgtcc gactggccaa ttccgatgag cgtcttggag      720

aagctcgaac taggtgagca caacgcggcg gcgcgtgtgg gattctccgt cgcggttggt      780

gagcatctcg cagcagggcc cacggcggtg cgcgaacgac tcgccgaggt ggggcgtctc      840

tctcggcagg tgctggcaga ggtcgacggg tggcgcgtcg tcgaacccgt cgaccaaccc      900

accgcgatca ccacccttga gtccaccgat ggtgccgatc ccgcgtcggt gcgctcgtgg      960

ctgatcgcgg agcgtggcat cgtgaccacc gcgtgtgaac tcgcgcgggc accgttcgag     1020

atgcgcacgc cggtgctgcg aatctcgccg cacgtcgacg tgacggtcga cgaactggag     1080

cagttcgccg cagcgttgcg tgaggcgccc tga                                  1113


<210>  8
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8

Met Leu Ala Gln Gln Trp Arg Asp Ala Arg Pro Lys Val Ala Gly Leu 
1               5                   10                  15      


His Leu Asp Ser Gly Ala Cys Ser Arg Gln Ser Phe Ala Val Ile Asp 
            20                  25                  30          


Ala Thr Thr Ala His Ala Arg His Glu Ala Glu Val Gly Gly Tyr Val 
        35                  40                  45              


Ala Ala Glu Ala Ala Thr Pro Ala Leu Asp Ala Gly Arg Ala Ala Val 
    50                  55                  60                  


Ala Ser Leu Ile Gly Phe Ala Ala Ser Asp Val Val Tyr Thr Ser Gly 
65                  70                  75                  80  


Ser Asn His Ala Ile Asp Leu Leu Leu Ser Ser Trp Pro Gly Lys Arg 
                85                  90                  95      


Thr Leu Ala Cys Leu Pro Gly Glu Tyr Gly Pro Asn Leu Ser Ala Met 
            100                 105                 110         


Ala Ala Asn Gly Phe Gln Val Arg Ala Leu Pro Val Asp Asp Asp Gly 
        115                 120                 125             


Arg Val Leu Val Asp Glu Ala Ser His Glu Leu Ser Ala His Pro Val 
    130                 135                 140                 


Ala Leu Val His Leu Thr Ala Leu Ala Ser His Arg Gly Ile Ala Gln 
145                 150                 155                 160 


Pro Ala Ala Glu Leu Val Glu Ala Cys His Asn Ala Gly Ile Pro Val 
                165                 170                 175     


Val Ile Asp Ala Ala Gln Ala Leu Gly His Leu Asp Cys Asn Val Gly 
            180                 185                 190         


Ala Asp Ala Val Tyr Ser Ser Ser Arg Lys Trp Leu Ala Gly Pro Arg 
        195                 200                 205             


Gly Val Gly Val Leu Ala Val Arg Pro Glu Leu Ala Glu Arg Leu Gln 
    210                 215                 220                 


Pro Arg Ile Pro Pro Ser Asp Trp Pro Ile Pro Met Ser Val Leu Glu 
225                 230                 235                 240 


Lys Leu Glu Leu Gly Glu His Asn Ala Ala Ala Arg Val Gly Phe Ser 
                245                 250                 255     


Val Ala Val Gly Glu His Leu Ala Ala Gly Pro Thr Ala Val Arg Glu 
            260                 265                 270         


Arg Leu Ala Glu Val Gly Arg Leu Ser Arg Gln Val Leu Ala Glu Val 
        275                 280                 285             


Asp Gly Trp Arg Val Val Glu Pro Val Asp Gln Pro Thr Ala Ile Thr 
    290                 295                 300                 


Thr Leu Glu Ser Thr Asp Gly Ala Asp Pro Ala Ser Val Arg Ser Trp 
305                 310                 315                 320 


Leu Ile Ala Glu Arg Gly Ile Val Thr Thr Ala Cys Glu Leu Ala Arg 
                325                 330                 335     


Ala Pro Phe Glu Met Arg Thr Pro Val Leu Arg Ile Ser Pro His Val 
            340                 345                 350         


Asp Val Thr Val Asp Glu Leu Glu Gln Phe Ala Ala Ala Leu Arg Glu 
        355                 360                 365             


Ala Pro 
    370 


<210>  9
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
agaattcaaa agatctaaag gaggccatcc atgatcgcac gcgagacac                   49


<210>  10
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10
actcgagttt ggatcctcag acgtcccagg ccaggcggac acccgagaat atc              53


<210>  11
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11
agaattcaaa agatctaaag gaggccatcc atgtgccggc atgtggcgtg                  50


<210>  12
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12
actcgagttt ggatcctcac aggggtgtca cgac                                   34


<210>  13
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13
agaattcaaa agatctaaag gaggccatcc atgacgctct cactggccaa c                51


<210>  14
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14
actcgagttt ggatcctcac cgcaccgcca gcgac                                  35


<210>  15
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15
agaattcaaa agatctaaag gaggccatcc atgctcgcgc agcagtg                     47


<210>  16
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16
actcgagttt ggatcctcag ggcgcctcac gcaac                                  35


