                               SEQUENCE LISTING

<110> BLOODWORKS
 
<120> STEAP3 AS A BIOCHEMICAL MARKER OF RED BLOOD CELL STORAGE AND 
      TOXICITY

<130> 29382/PCT

<140> PCT/US2015/029552
<141> 2015-05-06

<150> 61/989,440
<151> 2014-05-06

<160> 4     

<170> PatentIn version 3.5

<210> 1
<211> 488
<212> PRT
<213> Homo sapiens

<400> 1
Met Pro Glu Glu Met Asp Lys Pro Leu Ile Ser Leu His Leu Val Asp 
1               5                   10                  15      


Ser Asp Ser Ser Leu Ala Lys Val Pro Asp Glu Ala Pro Lys Val Gly 
            20                  25                  30          


Ile Leu Gly Ser Gly Asp Phe Ala Arg Ser Leu Ala Thr Arg Leu Val 
        35                  40                  45              


Gly Ser Gly Phe Lys Val Val Val Gly Ser Arg Asn Pro Lys Arg Thr 
    50                  55                  60                  


Ala Arg Leu Phe Pro Ser Ala Ala Gln Val Thr Phe Gln Glu Glu Ala 
65                  70                  75                  80  


Val Ser Ser Pro Glu Val Ile Phe Val Ala Val Phe Arg Glu His Tyr 
                85                  90                  95      


Ser Ser Leu Cys Ser Leu Ser Asp Gln Leu Ala Gly Lys Ile Leu Val 
            100                 105                 110         


Asp Val Ser Asn Pro Thr Glu Gln Glu His Leu Gln His Arg Glu Ser 
        115                 120                 125             


Asn Ala Glu Tyr Leu Ala Ser Leu Phe Pro Thr Cys Thr Val Val Lys 
    130                 135                 140                 


Ala Phe Asn Val Ile Ser Ala Trp Thr Leu Gln Ala Gly Pro Arg Asp 
145                 150                 155                 160 


Gly Asn Arg Gln Val Pro Ile Cys Gly Asp Gln Pro Glu Ala Lys Arg 
                165                 170                 175     


Ala Val Ser Glu Met Ala Leu Ala Met Gly Phe Met Pro Val Asp Met 
            180                 185                 190         


Gly Ser Leu Ala Ser Ala Trp Glu Val Glu Ala Met Pro Leu Arg Leu 
        195                 200                 205             


Leu Pro Ala Trp Lys Val Pro Thr Leu Leu Ala Leu Gly Leu Phe Val 
    210                 215                 220                 


Cys Phe Tyr Ala Tyr Asn Phe Val Arg Asp Val Leu Gln Pro Tyr Val 
225                 230                 235                 240 


Gln Glu Ser Gln Asn Lys Phe Phe Lys Leu Pro Val Ser Val Val Asn 
                245                 250                 255     


Thr Thr Leu Pro Cys Val Ala Tyr Val Leu Leu Ser Leu Val Tyr Leu 
            260                 265                 270         


Pro Gly Val Leu Ala Ala Ala Leu Gln Leu Arg Arg Gly Thr Lys Tyr 
        275                 280                 285             


Gln Arg Phe Pro Asp Trp Leu Asp His Trp Leu Gln His Arg Lys Gln 
    290                 295                 300                 


Ile Gly Leu Leu Ser Phe Phe Cys Ala Ala Leu His Ala Leu Tyr Ser 
305                 310                 315                 320 


Phe Cys Leu Pro Leu Arg Arg Ala His Arg Tyr Asp Leu Val Asn Leu 
                325                 330                 335     


Ala Val Lys Gln Val Leu Ala Asn Lys Ser His Leu Trp Val Glu Glu 
            340                 345                 350         


Glu Val Trp Arg Met Glu Ile Tyr Leu Ser Leu Gly Val Leu Ala Leu 
        355                 360                 365             


Gly Thr Leu Ser Leu Leu Ala Val Thr Ser Leu Pro Ser Ile Ala Asn 
    370                 375                 380                 


Ser Leu Asn Trp Arg Glu Phe Ser Phe Val Gln Ser Ser Leu Gly Phe 
385                 390                 395                 400 


Val Ala Leu Val Leu Ser Thr Leu His Thr Leu Thr Tyr Gly Trp Thr 
                405                 410                 415     


Arg Ala Phe Glu Glu Ser Arg Tyr Lys Phe Tyr Leu Pro Pro Thr Phe 
            420                 425                 430         


Thr Leu Thr Leu Leu Val Pro Cys Val Val Ile Leu Ala Lys Ala Leu 
        435                 440                 445             


Phe Leu Leu Pro Cys Ile Ser Arg Arg Leu Ala Arg Ile Arg Arg Gly 
    450                 455                 460                 


Trp Glu Arg Glu Ser Thr Ile Lys Phe Thr Leu Pro Thr Asp His Ala 
465                 470                 475                 480 


Leu Ala Glu Lys Thr Ser His Val 
                485             


<210> 2
<211> 3869
<212> DNA
<213> Homo sapiens

<400> 2
gaggaggagc ctcgggccga gccaccgcct tcgccgcgga ccttcagctg ccgcggtcgc       60

tccgagcggc gggccgcaga gccaccaaaa tgccagaaga gatggacaag ccactgatca      120

gcctccacct ggtggacagc gatagtagcc ttgccaaggt ccccgatgag gcccccaaag      180

tgggcatcct gggtagcggg gactttgccc gctccctggc cacacgcctg gtgggctctg      240

gcttcaaagt ggtggtgggg agccgcaacc ccaaacgcac agccaggctg tttccctcag      300

cggcccaagt gactttccaa gaggaggcag tgagctcccc ggaggtcatc tttgtggctg      360

tgttccggga gcactactct tcactgtgca gtctcagtga ccagctggcg ggcaagatcc      420

tggtggatgt gagcaaccct acagagcaag agcaccttca gcatcgtgag tccaatgctg      480

agtacctggc ctccctcttc cccacttgca cagtggtcaa ggccttcaat gtcatctctg      540

cctggaccct gcaggctggc ccaagggatg gtaacaggca ggtgcccatc tgcggtgacc      600

agccagaagc caagcgtgct gtctcggaga tggcgctcgc catgggcttc atgcccgtgg      660

acatgggatc cctggcgtca gcctgggagg tggaggccat gcccctgcgc ctcctcccgg      720

cctggaaggt gcccaccctg ctggccctgg ggctcttcgt ctgcttctat gcctacaact      780

tcgtccggga cgttctgcag ccctatgtgc aggaaagcca gaacaagttc ttcaagctgc      840

ccgtgtccgt ggtcaacacc acactgccgt gcgtggccta cgtgctgctg tcactcgtgt      900

acttgcccgg cgtgctggcg gctgccctgc agctgcggcg cggcaccaag taccagcgct      960

tccccgactg gctggaccac tggctacagc accgcaagca gatcgggctg ctcagcttct     1020

tctgcgccgc cctgcacgcc ctctacagct tctgcttgcc gctgcgccgc gcccaccgct     1080

acgacctggt caacctggca gtcaagcagg tcttggccaa caagagccac ctctgggtgg     1140

aggaggaggt ctggcggatg gagatctacc tctccctggg agtgctggcc ctcggcacgt     1200

tgtccctgct ggccgtgacc tcactgccgt ccattgcaaa ctcgctcaac tggagggagt     1260

tcagcttcgt tcagtcctca ctgggctttg tggccctcgt gctgagcaca ctgcacacgc     1320

tcacctacgg ctggacccgc gccttcgagg agagccgcta caagttctac ctgcctccca     1380

ccttcacgct cacgctgctg gtgccctgcg tcgtcatcct ggccaaagcc ctgtttctcc     1440

tgccctgcat cagccgcaga ctcgccagga tccggagagg ctgggagagg gagagcacca     1500

tcaagttcac gctgcccaca gaccacgccc tggccgagaa gacgagccac gtatgaggtg     1560

cctgccctgg gctctggacc ccgggcacac gagggacggt gccctgagcc cgttaggttt     1620

tcttttcttg gtggtgcaaa gtggtataac tgtgtgcaaa taggaggttt gaggtccaaa     1680

ttcctgggac tcaaatgtat gcagtactat tcagaatgat atacacacat atgtgtatat     1740

gtatttacat atattccaca tatataacag gatttgcaat tatacatagc tagctaaaaa     1800

gttgggtctc tgagatttca acttgtagat ttaaaaacaa gtgccgtacg ttaagagaag     1860

gcagatcatg ctattgtgac atttgcagag atatacacac actttttgta cagaagaggc     1920

ttgtgctgtg gtgggttcga tttatccctg cccaccccac ccccacaact tcccttttgc     1980

tacttcccca aggctcttgc agagctaggg ctctgaaggg gagggaaggc aacggctctg     2040

cccagagcca tccctggagc atgtgagcag cggctggtct cttccctcca cctggggcag     2100

cagcaggagg cctggggagg aggaaaatca ggcagtcggc ctggagtctg tgcctggtcc     2160

tttgcccggt ggtgggagga tggagggatt gggctgaagc tgctccacct catccttgct     2220

gagtggggga gacattttcc ctgaaagtca gaagtcacca tagagcctgc aaatggatcc     2280

tcctgtgaga gtgacgtcac ctcctttcca gagccattag tgagcctggc ttgggaacaa     2340

gtgtaatttc cttccctcct ttaacctggc gatgagcgtc ctttaaacca ctgtgccttc     2400

tcaccctttc catcttcagt ttgaatgact cccaggaagg cctagagcag accctttaga     2460

aatcagccca agggggagag caagagaaaa cactctaggg agtaaagctc cccgggcgtc     2520

agagttgagc cctgcctggg ctgaaggact gtcttcacga agtcagtcct gaggaaaaat     2580

attggggact ccaaatgtcc tctggcagag gacccagaaa accacactgg ctccaacttc     2640

ctcctcatgg ggcattacac ttcaaaacag tggggagcaa cttttccacc aaagctacaa     2700

acctaaaatg ctgctgcccc aaagcacaag agggaagagc accgccgggg ccacaggacg     2760

tctgtcctcc agtcacaggc catccttgct gctccctact gactctagct tacttcccct     2820

gtgaagaaac aggtgttctc ggctgagccc ccaaccctct gcagaaccag gttgatctgc     2880

cacagaaaaa gcatctttga agacaaagag ggtgaggtct tcatgagtct cctgggccca     2940

aagccatctt ctgatggaag gaagagagta gggccagtga aggctgccca gagagaatgt     3000

cacagatgag gctgcccctg cccccccccc gccagggagg tttcatgagc tcatgtctat     3060

gcagcacata agggttcttc agtgaaaagc aggagaagag cccactgcaa ggatagctca     3120

ttaggcacat gaccgatgca gggaaggcca tgccggggaa gctcttcctg caggtatttt     3180

ccatctgctg tgccaaggct gagcggcaga aacttgtctc ataaattggc actgatggag     3240

catcagctgt ggcccacaga gagccttgct gagaaggggg caggtaaagc agagatttta     3300

gcattgcctt ggcataacaa gggcccatcg attccctact aatgagaggc agggagagca     3360

tgggcaatgg agacccacca atgatcccca accccggtgg gtactggctg cctgccctgg     3420

gccagggaat ggctccttat accaaagatg ctggcacata gcagaaccca gtgcacgtcc     3480

tccccttccc acccacctct ggctgaaggt gctcaagagg gaagcaatta taaggtgggt     3540

ggcaggaggg aacaggtgcc acctgctgga caatcacacg aaaggcaggc gggctgtgta     3600

ctgggccctg actgtgcgtc cactgctgtc ttccctacct caccaggcta ctggcagcag     3660

catcccgaga gcacatcatc tccacagcct ggtaaattcc atgtgcctct gggtacaaaa     3720

gtgcctcaac gacatgctct ggaaatccca aatgccacag tctgaggttg atatctaaaa     3780

tctatgcctt caaaagagtc tctgtttttt ttttttaacc tggtagacag tataaaagca     3840

gtgcaaataa acacctaacc ttctgcaaa                                       3869


<210> 3
<211> 488
<212> PRT
<213> Mus musculus

<400> 3
Met Ser Gly Glu Met Asp Lys Pro Leu Ile Ser Arg Arg Leu Val Asp 
1               5                   10                  15      


Ser Asp Gly Ser Leu Ala Glu Val Pro Lys Glu Ala Pro Lys Val Gly 
            20                  25                  30          


Ile Leu Gly Ser Gly Asp Phe Ala Arg Ser Leu Ala Thr Arg Leu Val 
        35                  40                  45              


Gly Ser Gly Phe Ser Val Val Val Gly Ser Arg Asn Pro Lys Arg Thr 
    50                  55                  60                  


Ala Gly Leu Phe Pro Ser Leu Ala Gln Val Thr Phe Gln Glu Glu Ala 
65                  70                  75                  80  


Val Ser Ser Pro Glu Val Ile Phe Val Ala Val Phe Arg Glu His Tyr 
                85                  90                  95      


Ser Ser Leu Cys Ser Leu Ala Asp Gln Leu Ala Gly Lys Ile Leu Val 
            100                 105                 110         


Asp Val Ser Asn Pro Thr Glu Lys Glu His Leu Gln His Arg Gln Ser 
        115                 120                 125             


Asn Ala Glu Tyr Leu Ala Ser Leu Phe Pro Ala Cys Thr Val Val Lys 
    130                 135                 140                 


Ala Phe Asn Val Ile Ser Ala Trp Ala Leu Gln Ala Gly Pro Arg Asp 
145                 150                 155                 160 


Gly Asn Arg Gln Val Leu Ile Cys Ser Asp Gln Pro Glu Ala Lys Arg 
                165                 170                 175     


Thr Ile Ser Glu Met Ala Arg Ala Met Gly Phe Thr Pro Leu Asp Met 
            180                 185                 190         


Gly Ser Leu Ala Ser Ala Arg Glu Val Glu Ala Ile Pro Leu Arg Leu 
        195                 200                 205             


Leu Pro Ser Trp Lys Val Pro Thr Leu Leu Ala Leu Gly Leu Phe Val 
    210                 215                 220                 


Cys Phe Tyr Thr Tyr Asn Phe Ile Arg Asp Val Leu Gln Pro Tyr Ile 
225                 230                 235                 240 


Arg Lys Asp Glu Asn Lys Phe Tyr Lys Met Pro Leu Ser Val Val Asn 
                245                 250                 255     


Thr Thr Leu Pro Cys Val Ala Tyr Val Leu Leu Ser Leu Val Tyr Leu 
            260                 265                 270         


Pro Gly Val Leu Ala Ala Ala Leu Gln Leu Arg Arg Gly Thr Lys Tyr 
        275                 280                 285             


Gln Arg Phe Pro Asp Trp Leu Asp His Trp Leu Gln His Arg Lys Gln 
    290                 295                 300                 


Ile Gly Leu Leu Ser Phe Phe Phe Ala Met Leu His Ala Leu Tyr Ser 
305                 310                 315                 320 


Phe Cys Leu Pro Leu Arg Arg Ser His Arg Tyr Asp Leu Val Asn Leu 
                325                 330                 335     


Ala Val Lys Gln Val Leu Ala Asn Lys Ser Arg Leu Trp Ala Glu Glu 
            340                 345                 350         


Glu Val Trp Arg Met Glu Ile Tyr Leu Ser Leu Gly Val Leu Ala Leu 
        355                 360                 365             


Gly Met Leu Ser Leu Leu Ala Val Thr Ser Leu Pro Ser Ile Ala Asn 
    370                 375                 380                 


Ser Leu Asn Trp Lys Glu Phe Ser Phe Val Gln Ser Thr Leu Gly Phe 
385                 390                 395                 400 


Val Ala Leu Ile Leu Ser Thr Met His Thr Leu Thr Tyr Gly Trp Thr 
                405                 410                 415     


Arg Ala Phe Glu Glu Asn His Tyr Lys Phe Tyr Leu Pro Pro Thr Phe 
            420                 425                 430         


Thr Leu Thr Leu Leu Leu Pro Cys Val Ile Ile Leu Ala Lys Gly Leu 
        435                 440                 445             


Phe Leu Leu Pro Cys Leu Asn Arg Arg Leu Thr Lys Ile Arg Arg Gly 
    450                 455                 460                 


Trp Glu Lys Asp Gly Ala Val Lys Phe Met Leu Pro Gly Asp His Thr 
465                 470                 475                 480 


Gln Gly Glu Lys Thr Ser His Val 
                485             


<210> 4
<211> 3142
<212> DNA
<213> Mus musculus

<400> 4
tcacactcta tctcagacct tgagctaaag gactctccca aggcgagggg gccactgctt       60

actggggccc tccacctggg aaggagaggt taaaggtgac ccagggttaa ggagaacctg      120

gtatgccaca tctcaactta tgatgtgagc tccagccacc cagcttcaga agaaatggct      180

gcagaggccc acaggcagca gggctcttgc cccaccatcc cctcagaggg ctgtggaaag      240

tcaccagaga agaaaggcag tgcggccgac tctagacctg gtgagctgga gtctgtgggg      300

cagggaggaa gcagggcata gaagacacag gaagcaactc tgccctgatt ccagactcca      360

tctgcatgga ctgattccag gtgctagggt tcctttctca gaaaccccag aagtccacga      420

aggcactgct atgtcggggg agatggacaa gccgctgatc agccgccgcc tagtggacag      480

tgatggcagt ctggctgagg tccccaagga ggcccccaaa gtgggcatcc tgggcagtgg      540

ggattttgcc cgttccctgg ccacacgcct ggtgggctct ggcttcagtg tggtggtggg      600

gagccgtaac cccaaacgca cggctggcct cttcccctcc ttagctcaag tgactttcca      660

ggaggaagcc gtgagctctc cagaggtcat ctttgtggcc gtgttccggg agcactattc      720

ctcactgtgc agtctcgctg accagttggc tggcaagatc ctcgtggatg taagcaaccc      780

cacggagaag gagcatcttc agcaccgcca gtctaacgct gagtacctgg cctcactctt      840

tcctgcgtgc actgtggtga aggccttcaa cgtcatctct gcatgggccc tacaggctgg      900

cccaagggat gggaacaggc aggtgctcat ctgcagtgat cagccagaag ccaagcgcac      960

catctcagag atggcacgcg ccatgggttt cacacccctg gacatgggat ccctggcctc     1020

agcgagggag gtagaagcca tacccctgcg cctccttcca tcctggaagg tgcccaccct     1080

cctggcactg gggctctttg tgtgcttcta cacctacaac ttcatccgag acgttctaca     1140

gccatacatt cggaaagatg agaacaagtt ctacaagatg cccttgtctg tggtcaacac     1200

cacactaccc tgtgtggctt atgtgctgct gtccctagtg tacctgcccg gtgtgctggc     1260

agctgcgctt cagctgcgga gggggaccaa gtaccagcgc ttcccagact ggctggacca     1320

ctggctgcag catcgcaagc agatcgggct gctcagcttc ttcttcgcga tgctgcacgc     1380

tctctacagc ttctgcctgc cgctgcgccg ctcccaccgc tacgacctgg tcaatctggc     1440

tgtgaagcag gtcctggcca acaagagccg cctctgggct gaggaagaag tctggaggat     1500

ggagatatac ctgtccctgg gtgtgctggc cctgggcatg ttgtcgctgc tggctgtcac     1560

ctcgctcccg tccattgcta attccctcaa ctggaaggag ttcagcttcg tgcagtccac     1620

actgggcttc gtggccctga tactcagcac aatgcacaca ctcacctacg gctggacccg     1680

tgcctttgag gaaaaccact acaagttcta cctgccgccc acattcacac tcacgctgct     1740

cctgccctgt gtgatcatcc tggccaaggg cctcttcctc ctgccctgcc tcaaccgcag     1800

actcaccaag atacgcaggg gctgggagaa agatggggct gtcaagttca tgctgcccgg     1860

cgaccacaca cagggggaga aaacaagcca cgtgtgaggc cctggaagtg gagatggctt     1920

gtgggggccc tgagctgggt tcgggtctct tttctggatg ctgcacagcg aggtgatgat     1980

atatgcgtgg gtggctgaga tcctaattcc tgggatgcag gtgtaaactg acatactcag     2040

aatgacaccc catacatgtg atatgtactt acatatattt cacatataat aagatttgct     2100

attattctta cttagctaaa aaaaaaaagt gggtccctat atttcagcgt aagcatttca     2160

aagcaaatgc cacacattga acagcagatc ccacccttgt ggtatctaca gaggcagaca     2220

gacactctgg tataggagaa actgtctttc gttggattct ctcctttaat ctctatgctc     2280

cttattagct gaatcctaaa gttggtgcaa agctggggca agaaatgcct ctggtgccgc     2340

ctacccccat cccagggcta agaaagaagc ctcgagtgaa cagggaacca ggtctggact     2400

ctgctgcttc cctgggcgtg cgtggggagg ctcagcaaga ccccctggga tctatgcagg     2460

agctttttca ggtccgtcct ttcttcaggg aagggtctga agctgcccca tctgatccta     2520

gctgagctga gaagattctt ccccaccccc tgaaagtcca gagtcaccac cggagcctgc     2580

aaattgatcc ttctgcgaag gtgtgaagtc accgcctctc cagagccatt aatgaacctg     2640

gtcttcggga ggaggataat tgtttcctct ccattaagtt gctggtgacc ccccctttaa     2700

atcactgtgc cttctcgcct tttccatcat taatttggac atctccgtgg agtggacact     2760

tgtctgggca gtccggggtg ggggggagca ttagagattg cagagaataa ccatcgaatc     2820

ctcttcttgg ggcaaccctc cccttggatg tgccccaggc ctgccttcat taaattggtc     2880

cctgaggaga ataataggga cccttttcat ttaccctgtc gcctgtaggc agaaaaccta     2940

ccttctgagc acccagaaaa cacagtggcc ccatgctctt cttcaggggg ttccacagcc     3000

cccttccccg tgtttttgcc tccctccctc cttcctcccc tccctccctc cctcactgtt     3060

acgttcaacc acaaaagtct tcaaatattg tttttttgaa ttcttaaaga gacctcattt     3120

tattacaaaa aaaaaaaaaa aa                                              3142


