                         SEQUENCE LISTING

<110>  Wang, Li-Hsien
       Xue, Yingzi
       Murphy, Andrew J.
       Stevens, Sean
 
<120>  HUMANIZED IL-4 AND IL-4Ra ANIMALS

<130>  31260 (T0029)

<150>  61/989,757 
<151>  2014-05-07

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  1
catgcacgga gatggatgtg                                                   20


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  2
gacccctcag gtccacttac c                                                 21


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent prob

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Labled with BHQ: fluorescence quencher of the black hole quencher
       type

<400>  3
aacgtcctca cagcaacga                                                    19


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  4
gtgcccaggt gtgctcatg                                                    19


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  5
cgcctgcctc ctcactttat c                                                 21


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent probe

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  Labled with BHQ: fluorescence quencher of the black hole 
       quencher

       type

<400>  6
atctgcttca ccatccact                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  7
gcctggacca agactctgt                                                    19


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  8
accgtgggac ggcttcttac                                                   20


<210>  9
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent probe

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  Labled with BHQ: fluorescence quencher of the black hole quencher
       type

<400>  9
caccgagttg accgtaacag acatc                                             25


<210>  10
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  10
tgcggccgat cttagcc                                                      17


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  11
ttgaccgatt ccttgcgg                                                     18


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent probe

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Labled with BHQ: fluorescence quencher of the black hole quencher
       type

<400>  12
acgagcgggt tcggcccatt c                                                 21


<210>  13
<211>  458
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence containing the upstream junction of Murine 
       IL4 Locus and Human IL4 sequence

<400>  13
tgctgattgg cccagaataa ctgacaatct ggtgtaataa aattttccaa tgtaaactca       60

ttttcccttg gtttcagcaa ctttaactct atatatagag agacctctgc cagcattgca      120

ttgttagcat ctcttgataa acttaattgt ctctcgtcac tgacggcaca gagctattga      180

tgggtctcac ctcccaactg cttccccctc tgttcttcct gctagcatgt gccggcaact      240

ttgtccacgg acacaagtgc gatatcacct tacaggagat catcaaaact ttgaacagcc      300

tcacagagca gaaggtgagt acctatctgg caccatctct ccagatgttc tggtgatgct      360

ctcagtattt ctaggcatga aaacgttaac agctgctaga gaagttggaa ctggtggttg      420

gtggcagtcc agggcacaca gcgaggcttc tcccctgc                              458


<210>  14
<211>  565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence containing the downstream junction of Human
       IL4 sequence and floxed hygo selection cassett

<400>  14
tgtttatttt gcagaattcc tgtcctgtga aggaagccaa ccagagtacg ttggaaaact       60

tcttggaaag gctaaagacg atcatgagag agaaatattc aaagtgttcg agctgaatat      120

tttaatttat gagtttttga tagctttatt ttttaagtat ttatatattt ataactcatc      180

ataaaataaa gtatatatag aatctaacag caatggcatt taatgtattg gctatgttta      240

cttgacaaat gaaattatgg tttgcaactt ttagggaaat caatttagtt taccaagaga      300

ctataaatgc tatgggagca aaacaggaaa gaccacttcc ccctcgaggg gttccctctc      360

gagttaggga cataacacac aagataatta aagaacacaa ggccatacaa gatgcggccg      420

caccggtata acttcgtata aggtatccta tacgaagtta tatgcatggc ctccgcgccg      480

ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga      540

agggcgcagc gagcgtcctg atcct                                            565


<210>  15
<211>  438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence containing the downstream junction of 
       floxed hygo selection cassett and Murine IL-4 locus

<400>  15
tgccaagttc taattccatc agacctcgac ctgcagccgg cgcgccataa cttcgtataa       60

ggtatcctat acgaagttat ctcgagagga gttcccaccc ttctcaagag cataatgcgc      120

agatcattaa gggacagatg caggctgggg agacggttca gcagttagga gtacctgttg      180

ctcttccaga ggacccaggt tcaattcccg gcactcacat agcagcttaa aacaataact      240

caagttctgg gggagctgat gctctcctct ggcctcctgt ggaggtacac agaccacatg      300

cctgtaggca agacacccac acacataaaa acaaaataaa ataaggatag aaaggccagg      360

gggatgaatc cagaggtaga agaaaactta ttccctggaa ttgtcctctg actcccctcc      420

caaaacctct aacacgca                                                    438


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  16
ccgctggcat gtgtattgtg                                                   20


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  17
tgagtgtggg accctcaaga g                                                 21


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent probe

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Labled with BHQ: fluorescence quencher of the black hole quencher
       type

<400>  18
tgacccaagc cctacatggc cact                                              24


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  19
tgaggagagc tcacgggaat c                                                 21


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  20
acccatctcc tgcgtttctg                                                   20


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent probe

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Labled with BHQ: fluorescence quencher of the black hole quencher
       type

<400>  21
ttgacacgcc agctacactg ctcca                                             25


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  22
acctgcgtct ccgactacat g                                                 21


<210>  23
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  23
gagctcggtg ctgcaattg                                                    19


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent probe

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Labled with BHQ: fluorescence quencher of the black hole quencher
       type

<400>  24
tgggaccatt catcttccac tcgca                                             25


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  25
ggtggagagg ctattcggc                                                    19


<210>  26
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  26
gaacacggcg gcatcag                                                      17


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Labled with FAM: 5-carboxyfluorescein fluorescent probe

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  Labled with BHQ: fluorescence quencher of the black hole quencher
       type

<400>  27
tgggcacaac agacaatcgg ctg                                               23


<210>  28
<211>  243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of Murine IL-4Ralpha locus and sequence containing 
       IL-4Ralpha gene

<400>  28
tgggggaggg aggccatgac acaaatgaaa tggaccccgc tgacccagga tcagcatctg       60

cccactcttc tttctgcagg caccttttgt gtccccaatg gggtggcttt gctctgggct      120

cctgttccct gtgagctgcc tggtcctgct gcaggtggca agctctggta agtcaccact      180

tctcaatcat tcatttgttg gctattaatg gcgtgccagg gtcctgcagt atgtcacctg      240

gcc                                                                    243


<210>  29
<211>  243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence containing the downstream junction of Human
       IL-4Ralpha sequence and floxed neo selection cassett

<400>  29
gtcagatcgt ggagggtctc ggacgagggt cctgaccctg ggtctccagt cctgggaagt       60

ggagcccagg ctgtaccatg gctgacctca gctcatggct cccgggctcg ataactataa      120

cggtcctaag gtagcgactc gagataactt cgtataatgt atgctatacg aagttatatg      180

catggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg      240

ctg                                                                    243


<210>  30
<211>  390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of the sequence of the floxed neo selection cassett and 
       the Murine IL-4Ralpha locus

<400>  30
tattgttttg ccaagttcta attccatcag acctcgacct gcagccccta gataacttcg       60

tataatgtat gctatacgaa gttatcctag gttggagctc tctgtagcca ggtaaccaag      120

ggtcccaggg gaacccccag tgtggacgcg gactgcacat gacacagggc ggcctcccca      180

ttcatgactg tttttctcct tgcagacttc cagctgcccc tgatacagcg ccttccactg      240

ggggtcacca tctcctgcct ctgcatcccg ttgttttgcc tgttctgtta cttcagcatt      300

accaagtgag ttcctgcttt ggctggtgtc tctggctggc ccttcagcag tgctctcaga      360

ggtcacagtc attgtgctgg ctgagaaaag                                       390


<210>  31
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  31
ttggactagt aacaagaagg gtagca                                            26


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  32
cctttcccat caccctctaa ctt                                               23


<210>  33
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  33
agctctggtg gacaga                                                       16


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  34
tctctgccaa gctgcttatc c                                                 21


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  35
ggctgcatgg aagaggtgaa                                                   20


<210>  36
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  36
ctctccacaa atcg                                                         14


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  37
caggcaggaa tagctgagat aatct                                             25


<210>  38
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  38
tgtggagcaa aaagtggttg at                                                22


<210>  39
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  39
cctgtgaata gtgataaac                                                    19


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  40
cagttccaaa cgataggctc aa                                                22


<210>  41
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  41
ataattctgt gaagcatctg gtcttc                                            26


<210>  42
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  42
ctagagctgc tagtaaaa                                                     18


<210>  43
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  43
atagccaagg ttgcttctga tgacttcagg tccatatagt tggattaatg ttatatttca       60

atcccacaga aacctgaaaa atgaagccta aaatgaagta ttcaaccaac aaaatttcca      120

cagcaaagtg gaagaacaca gcaagcaaag ccttgtgttt                            160


<210>  44
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  44
tttatattat tgaataaagt atattttcca aatgtatgtg agactataat gattttatca       60

tatgatgact caatattctg ctcgagataa cttcgtataa tgtatgctat acgaagttat      120

atgcatggcc tccgcgccgg gttttggcgc ctcccgcggg                            160


<210>  45
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  45
agcccctaga taacttcgta taatgtatgc tatacgaagt tatgctagta actataacgg       60

tcctaaggta gcgagctagc cgcctgtgcg ttctgggttg aatgacttaa tgcttccaac      120

tgaagaaagg gtaacagaga gaaagaaagc cattcttggc                            160


<210>  46
<211>  237
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  46
tttatattat tgaataaagt atattttcca aatgtatgtg agactataat gattttatca       60

tatgatgact caatattctg ctcgagataa cttcgtataa tgtatgctat acgaagttat      120

gctagtaact ataacggtcc taaggtagcg agctagccgc ctgtgcgttc tgggttgaat      180

gacttaatgc ttccaactga agaaagggta acagagagaa agaaagccat tcttggc         237


