                         SEQUENCE LISTING


<110>  PhaseBio Pharmaceuticals, Inc.
             
       Arnold, Susan
 
       Ballance, David James


<120>  METHODS AND COMPOSITIONS FOR TREATING CYSTIC FIBROSIS

<130>  PHAS-030/01WO 309646- 

<140>  Submitted herewith
<141>  2015-05-08



<150>  US 61/990,425

<151>  2014-05-08




<160>  20    


<170>  PatentIn version 3.5


<210>  1
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  1

Val Pro Gly Gly 
1               


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  2

Ile Pro Gly Gly 
1               


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring or non-natural amino acid

<400>  3

Val Pro Gly Xaa Gly 
1               5   


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  4

Ala Val Gly Val Pro 
1               5   


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring or non-natural amino acid

<400>  5

Ile Pro Gly Xaa Gly 
1               5   


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  6

Ile Pro Gly Val Gly 
1               5   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring or non-natural amino acid

<400>  7

Leu Pro Gly Xaa Gly 
1               5   


<210>  8
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  8

Leu Pro Gly Val Gly 
1               5   


<210>  9
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  9

Val Ala Pro Gly Val Gly 
1               5       


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  10

Gly Val Gly Val Pro Gly Val Gly 
1               5               


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  11

Val Pro Gly Phe Gly Val Gly Ala Gly 
1               5                   


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence

<400>  12

Val Pro Gly Val Gly Val Pro Gly Gly 
1               5                   


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ELP component sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring or non-natural amino acid

<400>  13

Xaa Pro Gly Val Gly 
1               5   

<210>  14
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M-VIP

<400>  14

Met His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu Arg Lys 
1               5                   10                  15      


Gln Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn 
            20                  25                  


<210>  15
<211>  634
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M-VIP ELP1-120

<400>  15

Met His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu Arg Lys 
1               5                   10                  15      


Gln Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn Val Pro Gly 
            20                  25                  30          


Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala 
        35                  40                  45              


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly 
    50                  55                  60                  


Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val 
65                  70                  75                  80  


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro 
                85                  90                  95      


Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
            100                 105                 110         


Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly 
        115                 120                 125             


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly 
    130                 135                 140                 


Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
145                 150                 155                 160 


Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro 
                165                 170                 175     


Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
            180                 185                 190         


Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val 
        195                 200                 205             


Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly 
    210                 215                 220                 


Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
225                 230                 235                 240 


Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro 
                245                 250                 255     


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly 
            260                 265                 270         


Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val 
        275                 280                 285             


Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly 
    290                 295                 300                 


Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val 
305                 310                 315                 320 


Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro 
                325                 330                 335     


Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly 
            340                 345                 350         


Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly 
        355                 360                 365             


Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly 
    370                 375                 380                 


Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val 
385                 390                 395                 400 


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro 
                405                 410                 415     


Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly 
            420                 425                 430         


Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala 
        435                 440                 445             


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly 
    450                 455                 460                 


Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val 
465                 470                 475                 480 


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro 
                485                 490                 495     


Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
            500                 505                 510         


Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly 
        515                 520                 525             


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly 
    530                 535                 540                 


Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
545                 550                 555                 560 


Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro 
                565                 570                 575     


Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
            580                 585                 590         


Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val 
        595                 600                 605             


Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly 
    610                 615                 620                 


Val Pro Gly Gly Gly Val Pro Gly Trp Pro 
625                 630                 


<210>  16
<211>  636
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MAA-VIP ELP1-120

<400>  16

Met Ala Ala His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu 
1               5                   10                  15      


Arg Lys Gln Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn Val 
            20                  25                  30          


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro 
        35                  40                  45              


Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
    50                  55                  60                  


Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly 
65                  70                  75                  80  


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly 
                85                  90                  95      


Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
            100                 105                 110         


Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro 
        115                 120                 125             


Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
    130                 135                 140                 


Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val 
145                 150                 155                 160 


Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly 
                165                 170                 175     


Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
            180                 185                 190         


Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro 
        195                 200                 205             


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly 
    210                 215                 220                 


Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val 
225                 230                 235                 240 


Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly 
                245                 250                 255     


Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val 
            260                 265                 270         


Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro 
        275                 280                 285             


Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly 
    290                 295                 300                 


Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly 
305                 310                 315                 320 


Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly 
                325                 330                 335     


Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val 
            340                 345                 350         


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro 
        355                 360                 365             


Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly 
    370                 375                 380                 


Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala 
385                 390                 395                 400 


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly 
                405                 410                 415     


Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val 
            420                 425                 430         


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro 
        435                 440                 445             


Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
    450                 455                 460                 


Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly 
465                 470                 475                 480 


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly 
                485                 490                 495     


Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
            500                 505                 510         


Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro 
        515                 520                 525             


Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
    530                 535                 540                 


Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val 
545                 550                 555                 560 


Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly 
                565                 570                 575     


Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
            580                 585                 590         


Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro 
        595                 600                 605             


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly 
    610                 615                 620                 


Ala Gly Val Pro Gly Gly Gly Val Pro Gly Trp Pro 
625                 630                 635     




<210>  17
<211>  28
<212>  PRT
<213>  Homo sapiens

<400>  17

His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu Arg Lys Gln 
1               5                   10                  15      


Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn 
            20                  25              


<210>  18
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M-VIP-ELP1

<400>  18

Met His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu Arg Lys 
1               5                   10                  15      


Gln Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn Val Pro Gly 
            20                  25                  30          


Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala 
        35                  40                  45              


Gly Val 
    50  


<210>  19
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MAA-VIP

<400>  19

Met Ala Ala His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu 
1               5                   10                  15      


Arg Lys Gln Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn Val 
            20                  25                  30          


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro 
        35                  40                  45              


Gly Ala 
    50  



<210>  20
<211>  633
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VIP ELP1-120

<400>  20

His Ser Asp Ala Val Phe Thr Asp Asn Tyr Thr Arg Leu Arg Lys Gln 
1               5                   10                  15      


Met Ala Val Lys Lys Tyr Leu Asn Ser Ile Leu Asn Val Pro Gly Val 
            20                  25                  30          


Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly 
        35                  40                  45              


Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val 
    50                  55                  60                  


Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro 
65                  70                  75                  80  


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly 
                85                  90                  95      


Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val 
            100                 105                 110         


Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly 
        115                 120                 125             


Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val 
    130                 135                 140                 


Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro 
145                 150                 155                 160 


Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly 
                165                 170                 175     


Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly 
            180                 185                 190         


Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly  
        195                 200                 205             


Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val 
    210                 215                 220                 


Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro 
225                 230                 235                 240 


Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly
                245                 250                 255     


Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala 
            260                 265                 270         


Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly 
        275                 280                 285             


Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val 
    290                 295                 300                 


Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro 
305                 310                 315                 320 


Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly Val Pro Gly 
                325                 330                 335     


Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Val 
            340                 345                 350         


Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly 
        355                 360                 365             


Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val Gly Val 
    370                 375                 380                 


Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro 
385                 390                 395                 400 


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly 
                405                 410                 415     


Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro Gly Val
            420                 425                 430         


Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly 
        435                 440                 445             


Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val
    450                 455                 460                 


Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly Val Pro
465                 470                 475                 480 


Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly
                485                 490                 495     


Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Val 
            500                 505                 510         


Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly Gly Gly 
        515                 520                 525             


Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly Gly Val
    530                 535                 540                 


Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro 
545                 550                 555                 560 


Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val Pro Gly 
                565                 570                 575     


Gly Gly Val Pro Gly Val Gly Val Pro Gly Val Gly Val Pro Gly Gly
            580                 585                 590         


Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val Pro Gly Val Gly
        595                 600                 605             


Val Pro Gly Val Gly Val Pro Gly Gly Gly Val Pro Gly Ala Gly Val
    610                 615                 620                 


Pro Gly Gly Gly Val Pro Gly Trp Pro 
625                 630 



          

         



