                         SEQUENCE LISTING

<110>  Catalyst Therapeutics
 
<120>  Methods for inhibiting necroptosis

<130>  515172

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  471
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Glu Asn Leu Lys His Ile Ile Thr Leu Gly Gln Val Ile His Lys 
1               5                   10                  15      


Arg Cys Glu Glu Met Lys Tyr Cys Lys Lys Gln Cys Arg Arg Leu Gly 
            20                  25                  30          


His Arg Val Leu Gly Leu Ile Lys Pro Leu Glu Met Leu Gln Asp Gln 
        35                  40                  45              


Gly Lys Arg Ser Val Pro Ser Glu Lys Leu Thr Thr Ala Met Asn Arg 
    50                  55                  60                  


Phe Lys Ala Ala Leu Glu Glu Ala Asn Gly Glu Ile Glu Lys Phe Ser 
65                  70                  75                  80  


Asn Arg Ser Asn Ile Cys Arg Phe Leu Thr Ala Ser Gln Asp Lys Ile 
                85                  90                  95      


Leu Phe Lys Asp Val Asn Arg Lys Leu Ser Asp Val Trp Lys Glu Leu 
            100                 105                 110         


Ser Leu Leu Leu Gln Val Glu Gln Arg Met Pro Val Ser Pro Ile Ser 
        115                 120                 125             


Gln Gly Ala Ser Trp Ala Gln Glu Asp Gln Gln Asp Ala Asp Glu Asp 
    130                 135                 140                 


Arg Arg Ala Phe Gln Met Leu Arg Arg Asp Asn Glu Lys Ile Glu Ala 
145                 150                 155                 160 


Ser Leu Arg Arg Leu Glu Ile Asn Met Lys Glu Ile Lys Glu Thr Leu 
                165                 170                 175     


Arg Gln Tyr Leu Pro Pro Lys Cys Met Gln Glu Ile Pro Gln Glu Gln 
            180                 185                 190         


Ile Lys Glu Ile Lys Lys Glu Gln Leu Ser Gly Ser Pro Trp Ile Leu 
        195                 200                 205             


Leu Arg Glu Asn Glu Val Ser Thr Leu Tyr Lys Gly Glu Tyr His Arg 
    210                 215                 220                 


Ala Pro Val Ala Ile Lys Val Phe Lys Lys Leu Gln Ala Gly Ser Ile 
225                 230                 235                 240 


Ala Ile Val Arg Gln Thr Phe Asn Lys Glu Ile Lys Thr Met Lys Lys 
                245                 250                 255     


Phe Glu Ser Pro Asn Ile Leu Arg Ile Phe Gly Ile Cys Ile Asp Glu 
            260                 265                 270         


Thr Val Thr Pro Pro Gln Phe Ser Ile Val Met Glu Tyr Cys Glu Leu 
        275                 280                 285             


Gly Thr Leu Arg Glu Leu Leu Asp Arg Glu Lys Asp Leu Thr Leu Gly 
    290                 295                 300                 


Lys Arg Met Val Leu Val Leu Gly Ala Ala Arg Gly Leu Tyr Arg Leu 
305                 310                 315                 320 


His His Ser Glu Ala Pro Glu Leu His Gly Lys Ile Arg Ser Ser Asn 
                325                 330                 335     


Phe Leu Val Thr Gln Gly Tyr Gln Val Lys Leu Ala Gly Phe Glu Leu 
            340                 345                 350         


Arg Lys Thr Gln Thr Ser Met Ser Leu Gly Thr Thr Arg Glu Lys Thr 
        355                 360                 365             


Asp Arg Val Lys Ser Thr Ala Tyr Leu Ser Pro Gln Glu Leu Glu Asp 
    370                 375                 380                 


Val Phe Tyr Gln Tyr Asp Val Lys Ser Glu Ile Tyr Ser Phe Gly Ile 
385                 390                 395                 400 


Val Leu Trp Glu Ile Ala Thr Gly Asp Ile Pro Phe Gln Gly Cys Asn 
                405                 410                 415     


Ser Glu Lys Ile Arg Lys Leu Val Ala Val Lys Arg Gln Gln Glu Pro 
            420                 425                 430         


Leu Gly Glu Asp Cys Pro Ser Glu Leu Arg Glu Ile Ile Asp Glu Cys 
        435                 440                 445             


Arg Ala His Asp Pro Ser Val Arg Pro Ser Val Asp Glu Ile Leu Lys 
    450                 455                 460                 


Lys Leu Ser Thr Phe Ser Lys 
465                 470     


<210>  2
<211>  263
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Glu Asn Leu Lys His Ile Ile Thr Leu Gly Gln Val Ile His Lys 
1               5                   10                  15      


Arg Cys Glu Glu Met Lys Tyr Cys Lys Lys Gln Cys Arg Arg Leu Gly 
            20                  25                  30          


His Arg Val Leu Gly Leu Ile Lys Pro Leu Glu Met Leu Gln Asp Gln 
        35                  40                  45              


Gly Lys Arg Ser Val Pro Ser Glu Lys Leu Thr Thr Ala Met Asn Arg 
    50                  55                  60                  


Phe Lys Ala Ala Leu Glu Glu Ala Asn Gly Glu Ile Glu Lys Phe Ser 
65                  70                  75                  80  


Asn Arg Ser Asn Ile Cys Arg Phe Leu Thr Ala Ser Gln Asp Lys Ile 
                85                  90                  95      


Leu Phe Lys Asp Val Asn Arg Lys Leu Ser Asp Val Trp Lys Glu Leu 
            100                 105                 110         


Ser Leu Leu Leu Gln Val Glu Gln Arg Met Pro Val Ser Pro Ile Ser 
        115                 120                 125             


Gln Gly Ala Ser Trp Ala Gln Glu Asp Gln Gln Asp Ala Asp Glu Asp 
    130                 135                 140                 


Arg Arg Ala Phe Gln Met Leu Arg Arg Asp Asn Glu Lys Ile Glu Ala 
145                 150                 155                 160 


Ser Leu Arg Arg Leu Glu Ile Asn Met Lys Glu Ile Lys Glu Thr Leu 
                165                 170                 175     


Arg Gln Ser Leu Glu Ser Ser Ser Gly Lys Ser Pro Leu Glu Ile Ser 
            180                 185                 190         


Arg Phe Lys Val Lys Asn Val Lys Thr Gly Ser Ala Ser Gly Cys Asn 
        195                 200                 205             


Ser Glu Lys Ile Arg Lys Leu Val Ala Val Lys Arg Gln Gln Glu Pro 
    210                 215                 220                 


Leu Gly Glu Asp Cys Pro Ser Glu Leu Arg Glu Ile Ile Asp Glu Cys 
225                 230                 235                 240 


Arg Ala His Asp Pro Ser Val Arg Pro Ser Val Asp Glu Ile Leu Lys 
                245                 250                 255     


Lys Leu Ser Thr Phe Ser Lys 
            260             


<210>  3
<211>  472
<212>  PRT
<213>  Mus musculus

<400>  3

Met Asp Lys Leu Gly Gln Ile Ile Lys Leu Gly Gln Leu Ile Tyr Glu 
1               5                   10                  15      


Gln Cys Glu Lys Met Lys Tyr Cys Arg Lys Gln Cys Gln Arg Leu Gly 
            20                  25                  30          


Asn Arg Val His Gly Leu Leu Gln Pro Leu Gln Arg Leu Gln Ala Gln 
        35                  40                  45              


Gly Lys Lys Asn Leu Pro Asp Asp Ile Thr Ala Ala Leu Gly Arg Phe 
    50                  55                  60                  


Asp Glu Val Leu Lys Glu Ala Asn Gln Gln Ile Glu Lys Phe Ser Lys 
65                  70                  75                  80  


Lys Ser His Ile Trp Lys Phe Val Ser Val Gly Asn Asp Lys Ile Leu 
                85                  90                  95      


Phe His Glu Val Asn Glu Lys Leu Arg Asp Val Trp Glu Glu Leu Leu 
            100                 105                 110         


Leu Leu Leu Gln Val Tyr His Trp Asn Thr Val Ser Asp Val Ser Gln 
        115                 120                 125             


Pro Ala Ser Trp Gln Gln Glu Asp Arg Gln Asp Ala Glu Glu Asp Gly 
    130                 135                 140                 


Asn Glu Asn Met Lys Val Ile Leu Met Gln Leu Gln Ile Ser Val Glu 
145                 150                 155                 160 


Glu Ile Asn Lys Thr Leu Lys Gln Cys Ser Leu Lys Pro Thr Gln Glu 
                165                 170                 175     


Ile Pro Gln Asp Leu Gln Ile Lys Glu Ile Pro Lys Glu His Leu Gly 
            180                 185                 190         


Pro Pro Trp Thr Lys Leu Lys Thr Ser Lys Met Ser Thr Ile Tyr Arg 
        195                 200                 205             


Gly Glu Tyr His Arg Ser Pro Val Thr Ile Lys Val Phe Asn Asn Pro 
    210                 215                 220                 


Gln Ala Glu Ser Val Gly Ile Val Arg Phe Thr Phe Asn Asp Glu Ile 
225                 230                 235                 240 


Lys Thr Met Lys Lys Phe Asp Ser Pro Asn Ile Leu Arg Ile Phe Gly 
                245                 250                 255     


Ile Cys Ile Asp Gln Thr Val Lys Pro Pro Glu Phe Ser Ile Val Met 
            260                 265                 270         


Glu Tyr Cys Glu Leu Gly Thr Leu Arg Glu Leu Leu Asp Arg Glu Lys 
        275                 280                 285             


Asp Leu Thr Met Ser Val Arg Ser Leu Leu Val Leu Arg Ala Ala Arg 
    290                 295                 300                 


Gly Leu Tyr Arg Leu His His Ser Glu Thr Leu His Arg Asn Ile Ser 
305                 310                 315                 320 


Ser Ser Ser Phe Leu Val Ala Gly Gly Tyr Gln Val Lys Leu Ala Gly 
                325                 330                 335     


Phe Glu Leu Ser Lys Thr Gln Asn Ser Ile Ser Arg Thr Ala Lys Ser 
            340                 345                 350         


Thr Lys Ala Glu Arg Ser Ser Ser Thr Ile Tyr Val Ser Pro Glu Arg 
        355                 360                 365             


Leu Lys Asn Pro Phe Cys Leu Tyr Asp Ile Lys Ala Glu Ile Tyr Ser 
    370                 375                 380                 


Phe Gly Ile Val Leu Trp Glu Ile Ala Thr Gly Lys Ile Pro Phe Glu 
385                 390                 395                 400 


Gly Cys Asp Ser Lys Lys Ile Arg Glu Leu Val Ala Glu Asp Lys Lys 
                405                 410                 415     


Gln Glu Pro Val Gly Gln Asp Cys Pro Glu Leu Leu Arg Glu Ile Ile 
            420                 425                 430         


Asn Glu Cys Arg Ala His Glu Pro Ser Gln Arg Pro Ser Val Asp Gly 
        435                 440                 445             


Arg Ser Leu Ser Gly Arg Glu Arg Ile Leu Glu Arg Leu Ser Ala Val 
    450                 455                 460                 


Glu Glu Ser Thr Asp Lys Lys Val 
465                 470         


<210>  4
<211>  464
<212>  PRT
<213>  Mus musculus

<400>  4

Met Asp Lys Leu Gly Gln Ile Ile Lys Leu Gly Gln Leu Ile Tyr Glu 
1               5                   10                  15      


Gln Cys Glu Lys Met Lys Tyr Cys Arg Lys Gln Cys Gln Arg Leu Gly 
            20                  25                  30          


Asn Arg Val His Gly Leu Leu Gln Pro Leu Gln Arg Leu Gln Ala Gln 
        35                  40                  45              


Gly Lys Lys Asn Leu Pro Asp Asp Ile Thr Ala Ala Leu Gly Arg Phe 
    50                  55                  60                  


Asp Glu Val Leu Lys Glu Ala Asn Gln Gln Ile Glu Lys Phe Ser Lys 
65                  70                  75                  80  


Lys Ser His Ile Trp Lys Phe Val Ser Val Gly Asn Asp Lys Ile Leu 
                85                  90                  95      


Phe His Glu Val Asn Glu Lys Leu Arg Asp Val Trp Glu Glu Leu Leu 
            100                 105                 110         


Leu Leu Leu Gln Val Tyr His Trp Asn Thr Val Ser Asp Val Ser Gln 
        115                 120                 125             


Pro Ala Ser Trp Gln Gln Glu Asp Arg Gln Asp Ala Glu Glu Asp Gly 
    130                 135                 140                 


Asn Glu Asn Met Lys Val Ile Leu Met Gln Leu Gln Ile Ser Val Glu 
145                 150                 155                 160 


Glu Ile Asn Lys Thr Leu Lys Gln Cys Ser Leu Lys Pro Thr Gln Glu 
                165                 170                 175     


Ile Pro Gln Asp Leu Gln Ile Lys Glu Ile Pro Lys Glu His Leu Gly 
            180                 185                 190         


Pro Pro Trp Thr Lys Leu Lys Thr Ser Lys Met Ser Thr Ile Tyr Arg 
        195                 200                 205             


Gly Glu Tyr His Arg Ser Pro Val Thr Ile Lys Val Phe Asn Asn Pro 
    210                 215                 220                 


Gln Ala Glu Ser Val Gly Ile Val Arg Phe Thr Phe Asn Asp Glu Ile 
225                 230                 235                 240 


Lys Thr Met Lys Lys Phe Asp Ser Pro Asn Ile Leu Arg Ile Phe Gly 
                245                 250                 255     


Ile Cys Ile Asp Gln Thr Val Lys Pro Pro Glu Phe Ser Ile Val Met 
            260                 265                 270         


Glu Tyr Cys Glu Leu Gly Thr Leu Arg Glu Leu Leu Asp Arg Glu Lys 
        275                 280                 285             


Asp Leu Thr Met Ser Val Arg Ser Leu Leu Val Leu Arg Ala Ala Arg 
    290                 295                 300                 


Gly Leu Tyr Arg Leu His His Ser Glu Thr Leu His Arg Asn Ile Ser 
305                 310                 315                 320 


Ser Ser Ser Phe Leu Val Ala Gly Gly Tyr Gln Val Lys Leu Ala Gly 
                325                 330                 335     


Phe Glu Leu Ser Lys Thr Gln Asn Ser Ile Ser Arg Thr Ala Lys Ser 
            340                 345                 350         


Thr Lys Ala Glu Arg Ser Ser Ser Thr Ile Tyr Val Ser Pro Glu Arg 
        355                 360                 365             


Leu Lys Asn Pro Phe Cys Leu Tyr Asp Ile Lys Ala Glu Ile Tyr Ser 
    370                 375                 380                 


Phe Gly Ile Val Leu Trp Glu Ile Ala Thr Gly Lys Ile Pro Phe Glu 
385                 390                 395                 400 


Gly Cys Asp Ser Lys Lys Ile Arg Glu Leu Val Ala Glu Asp Lys Lys 
                405                 410                 415     


Gln Glu Pro Val Gly Gln Asp Cys Pro Glu Leu Leu Arg Glu Ile Ile 
            420                 425                 430         


Asn Glu Cys Arg Ala His Glu Pro Ser Gln Arg Pro Ser Val Asp Gly 
        435                 440                 445             


Ile Leu Glu Arg Leu Ser Ala Val Glu Glu Ser Thr Asp Lys Lys Val 
    450                 455                 460                 


