                         SEQUENCE LISTING

<110>  METABOLIC EXPLORER
 
<120>  New microorganism and method for the production of 
       1,2-propanediol based on NADPH dependent acetol reductase and 
       improved NADPH supply

<130>  B367604D33761

<150>  EP14305691.9
<151>  2014-05-12

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  101
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for frdABCD

<400>  1
cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc       60

tgcaattgct gccgcgcagg ccatatgaat atcctcctta g                          101


<210>  2
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for frdABCD

<400>  2
cgttagattg taacgacacc aatcagcgtg acaactgtca ggatagcagc cagaccgtag       60

aaaacccatt tgcccgcagg tgtaggctgg agctgcttcg                            100


<210>  3
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for ptsG

<400>  3
atgtttaaga atgcatttgc taacctgcaa aaggtcggta aatcgctgat gctgccggta       60

tccgtactgc ctatcgcagg tgtaggctgg agctgcttcg                            100


<210>  4
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for ptsG

<400>  4
ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa       60

aatcgcctga acaccagaac catatgaata tcctccttag                            100


<210>  5
<211>  101
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for gldA

<400>  5
atggaccgca ttattcaatc accgggtaaa tacatccagg gcgctgatgt gattaatcgt       60

ctgggcgaat acctgaagcc gtgtaggctg gagctgcttc g                          101


<210>  6
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for gldA

<400>  6
ttattcccac tcttgcagga aacgctgacc gtactggtcg gctaccagca gagcggcgta       60

aacctgatct ggcgtcgcgc catatgaata tcctccttag                            100


<210>  7
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Ribosome binding site RBS120

<400>  7
atccggtata ggaggtatag a                                                 21


<210>  8
<211>  261
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence for recombination into pntAB

<400>  8
gaccgtcgaa gacaattatc agtctttatc cggcgttcta aggtgtttat cccactatca       60

cggctgaatc gttaatattt tgcgagttca cgccgaaata ctgatttttg gcgctagatc      120

acaggcataa ttttcagtac gttatagggc gtttgttact aatttatttt aacggagtaa      180

catttagctc gtacatgagc agcttgtgtg gctcctgaca caggcaaacc atcatcaata      240

aaaccgatgg aagggaatat c                                                261


<210>  9
<211>  260
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence for recombination into pntAB

<400>  9
atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc agcaacgcca       60

aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag cggcgcgggt      120

caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg      180

aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt      240

gcgttactga atcctgggac                                                  260


<210>  10
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Ribosome binding site RBS121

<400>  10
agacaataat cgaacaacat attaaggaga gttt                                   34


<210>  11
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotides for Dgpi

<400>  11
ccaacgcaga ccgctgcctg gcaggcacta cagaaacact tcgatgaaat gaaagacgtt       60

acgatcgccg atctttttgc tgtaggctgg agctgcttcg                            100


<210>  12
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotides for Dgpi

<400>  12
gcgccacgct ttatagcggt taatcagacc attggtcgag ctatcgtggc tgctgatttc       60

tttatcatct ttcagctctg catatgaata tcctccttag                            100


<210>  13
<211>  101
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for pfkA

<400>  13
gttcctcggt tctgcgcgtt tcccggaatt ccgcgacgag aacatccgcg ccgtggctat       60

cgaaaacctg aaaaaacgtg gtgtaggctg gagctgcttc g                          101


<210>  14
<211>  102
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for pfkA

<400>  14
ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc agggtaaagg       60

aatctgcctt tttccgaaat cacatatgaa tatcctcctt ag                         102


<210>  15
<211>  81
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotides for recombination into Pfka

<400>  15
gttcctcggt tctgcgcgtt tcccggaatt ccgcgacgag aacatccgcg ccgtggctat       60

cgaaaacctg aaaaaacgtg g                                                 81


<210>  16
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotides for recombination into Pfka

<400>  16
ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc agggtaaagg       60

aatctgcctt tttccgaaat ca                                                82


<210>  17
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Ribosome binding site RBS113

<400>  17
aactcatttc gtttttaggg aggaataa                                          28


<210>  18
<211>  202
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence for recombination into ykiA

<400>  18
cttccccttg aacgggaggg catttttctg aaatatcctt tctttagccc ataataatat       60

ttcctttgct gcgatttttt caatttccga tatattcata atttatcaag gttgatataa      120

atatcagtga agatctccag atattgttgc ggaactggct acgataaaag ataaatcaga      180

tgatgaatgg tggcgtgcat tg                                               202


<210>  19
<211>  254
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence for recombination into ykiA

<400>  19
catcctgatg actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg       60

cgaagcacaa cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct      120

tatccggcgt acgaagccac accaggcata taattattcg ctacggcgag caataatttt      180

tagcgcagca atattatgcg ttttacgctg taacttgctc catggacgtt gtgtcattgt      240

ttttcctcaa gccg                                                        254


<210>  20
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for lpd

<400>  20
atgagtactg aaatcaaaac tcaggtcgtg gtacttgggg caggccccgc aggttactcc       60

gctgccttcc gttgcgctga catatgaata tcctccttag                            100


<210>  21
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for lpd

<400>  21
ttacttcttc ttcgctttcg ggttcggcag gtcggtaatg ctaccttcga acacttctgc       60

cgccaggccc acagactcgt tgtaggctgg agctgcttcg                            100


<210>  22
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Ribosome binding site RBS131

<400>  22
tggcaaggta agcaaactat aaggaggtca aat                                    33


<210>  23
<211>  1013
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic gene gld

<400>  23
aagctttgat ggcaagcgat accattcgta ttccgggtat tgatacaccg ctgagccgtg       60

ttgcactggg cacctgggca attggtggtt ggatgtgggg tggtccggat gatgataatg      120

gtgttcgtac cattcatgca gcactggatg aaggtattaa tctgattgat accgctccgg      180

tttatggttt tggtcatagc gaagaaattg ttggtcgtgc actggcagaa aaaccgaata      240

aagcacatgt tgcaaccaaa ctgggtctgc attgggttgg tgaagatgag aaaaacatga      300

aagtgtttcg tgatagccgt ccggcacgta ttcgtaaaga agttgaagat agcctgcgtc      360

gtctgcgtgt tgaaaccatt gatctggaac aaattcattg gcctgatgat aaaaccccga      420

ttgatgaaag cgcacgtgaa ctgcagaaac tgcatcagga tggtaaaatt cgtgccctgg      480

gtgttagcaa ttttagtccg gaacaaatgg atatctttcg tgaagttgca ccgctggcaa      540

ccattcagcc tccgctgaac ctgtttgaac gtaccattga aaaagatatt ctgccgtatg      600

ccgaaaaaca taatgcagtt gttctggcat atggtgcact gtgtcgtggt ctgctgaccg      660

gcaaaatgaa tcgtgatacc acctttccga aagatgatct gcgtagcaat gatccgaaat      720

ttcagaaacc gaacttcgag aaatatctgg ctgcaatgga tgagtttgaa aaactggccg      780

agaaacgtgg taaaagcgtt atggcatttg cagttcgttg ggttctggat cagggtccgg      840

ttattgcact gtggggtgca cgtaaaccgg gtcaggttag cggtgttaaa gatgtttttg      900

gttggagcct gaccgacgaa gaaaaaaaag cagttgatga tattctggca cgtcatgttc      960

cgaatccgat tgatccgacc tttatggcac cgcctgcacg tgattaagaa ttc            1013


<210>  24
<211>  971
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic gene gld2

<400>  24
aagctttgat ggcctccaag acgtacactc tgaacaccgg tgccaagata cccgcggtcg       60

ggttcggcac attcgccaat gagggtgcca agggcgagac atacgcagct gttacaaagg      120

cactggacgt tggataccgc caccttgatt gcgcgtggtt ttaccacaac gaagatgagg      180

ttggtgacgc ggtacgcgat tttctcgccc gccgacccga cgtgaaacgc gaggatctct      240

tcatttgcac caaagtttgg aaccacctgc atgagccaga ggacgtcaag tggagcgcca      300

agaactcgtg cgaaaacctc aaggtcgatt acattgacct gttcctcgtc cactggccaa      360

tcgcggccga gaagaacagc gacaggagcg tcaagctggg ccccgatggc aagtatgtca      420

tcaaccaagc cctgacggaa aacccagagc caacatggcg agccatggaa gagcttgttg      480

aaagcggcct cgtcaaggca attggagtat ccaactggac gattccgggg ttgaagaagc      540

tccttcagat cgccaagatc aagccggcag tgaaccagat tgagattcac ccattcctac      600

caaacgaaga gcttgtggcg ttctgctttg agaacgggat cctgcccgaa gcctactcgc      660

cgctgggctc gcagaaccag gtcccaagca ccggcgagcg agtgcgcgac aacccgacac      720

tcaaagcggt tgccgagcga agcggctaca gccttgccca gatcctattg gcatggggcc      780

tgaagcgagg atatgtggtc ctcccaaaga gctcaactcc aagccgtatt gaaagcaact      840

tcaacattcc ggagctgagt gatgaagact ttgaggcgat tcaacaggtt gctaagggga      900

gacatactag atttgtcaac atgaaggaca cgtttggata caacgtttgg ccagaggagg      960

aataagaatt c                                                           971


<210>  25
<211>  1103
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic gene adh1

<400>  25
aagctttgac tagtatgaaa ggacttgcta tgcttggaat tggaagaatt ggatggattg       60

aaaagaaaat cccagaatgt ggaccacttg atgcattagt tagaccatta gcacttgcac      120

catgtacatc agatacacat accgtttggg caggagctat tggagataga catgatatga      180

ttcttggaca tgaagcggtt ggacaaattg ttaaagttgg atcattagtt aagagattaa      240

aagttggaga taaagttatt gtaccagcta ttacaccaga ttggggagaa gaagaatcgc      300

aaagaggata tccaatgcat tcaggaggaa tgcttggagg atggaaattc tcaaatttca      360

aggatggagt tttttcagaa gttttccatg ttaatgaagc agatgccaat cttgcacttc      420

ttccaagaga tattaaacca gaagatgcag ttatgttatc agatatggta actactggat      480

tccatggagc agaattagct aatattaaac ttggagatac tgtttgtgtt attggtattg      540

gaccagttgg attaatgtca gttgcaggag caaaccatct tggagcagga agaatctttg      600

cagtaggatc aagaaaacat tgttgtgata ttgcattgga atatggagca acagatatta      660

ttaattataa aaatggagat attgtagaac aaattcttaa agctacagac ggcaaaggag      720

ttgataaagt cgttattgca ggaggtgatg ttcatacatt tgcacaagca gtcaaaatga      780

ttaaaccagg atcagatatt ggaaatgtta attatcttgg agaaggagat aatattgata      840

ttccaagaag tgaatgggga gttggaatgg gtcataaaca cattcatgga ggtttaaccc      900

caggtggaag agtcagaatg gaaaaattag catcacttat ttcaactggt aaattagata      960

cttctaaact tattacacat agatttgaag gattagaaaa agttgaagat gcattaatgt     1020

taatgaagaa taaaccagca gaccttatca aaccagttgt cagaattcat tatgatgatg     1080

aagatactct tcattaactc gag                                             1103


<210>  26
<211>  101
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for yqhDE

<400>  26
atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct       60

ggtttacgcg aacaaattcc gtgtaggctg gagctgcttc g                          101


<210>  27
<211>  99
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide for yqhDE

<400>  27
ttagccgccg aactggtcag gatcgggacc gagacgcttg ccctgatcga gttttgcaat       60

ttcgccgagt tcgtctttgc atatgaatat cctccttag                              99


