                         SEQUENCE LISTING

<110>  Vrije Universiteit Brussel
       Vandendriessche, Thierry
       Chuah, Marinee
 
<120>  Genetic correction of myotonic dystrophy type I

<130>  VUB2338PCT

<150>  EP14168688.1
<151>  2014-05-16

<150>  EP14168708.7
<151>  2014-05-16

<160>  126   

<170>  PatentIn version 3.5

<210>  1
<211>  2099
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left TALE 1755

<400>  1
atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat caagcctaag       60

gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt cactcatgcg      120

catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt caaataccaa      180

gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag      240

tggtcgggag cgcgagcact tgaggcgctg ctgactgtgg cgggtgagct tagggggcct      300

ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg agtaacagcg      360

gtagaggcag tgcacgcctg gcgcaatgcg ctcaccgggg cccccttgaa cctgacccca      420

gaccaggtag tcgcaatcgc gaacaataat gggggaaagc aagccctgga aaccgtgcaa      480

aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt      540

gcaaataata acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc      600

tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtcgaa cattggaggg      660

aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg      720

acgcctgcac aagtggtcgc catcgcctcc aatattggcg gtaagcaggc gctggaaaca      780

gtacagcgcc tgctgcctgt actgtgccag gatcatggac tgaccccaga ccaggtagtc      840

gcaatcgcga acaataatgg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg      900

gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aagcaacatc      960

ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac     1020

gggctgactc ccgatcaagt tgtagcgatt gcgtcgcatg acggagggaa acaagcattg     1080

gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa     1140

gtggtcgcca tcgcctcgaa tggcggcggt aagcaggcgc tggaaacagt acagcgcctg     1200

ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc aatcgcgaac     1260

aataatgggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa     1320

gaccacggcc ttacaccgga gcaagtcgtg gccattgcaa gcaacatcgg tggcaaacag     1380

gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc     1440

gatcaagttg tagcgattgc gaataacaat ggagggaaac aagcattgga gactgtccaa     1500

cggctccttc ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc     1560

gcctcgaatg gcggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg     1620

tgccaggatc atggactgac cccagaccag gtagtcgcaa tcgcgaacaa taatggggga     1680

aagcaagccc tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt     1740

acaccggagc aagtcgtggc cattgcatcc cacgacggtg gcaaacaggc tcttgagacg     1800

gttcagagac ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta     1860

gcgattgcgt cgcatgacgg agggaaacaa gcattggaga ctgtccaacg gctccttccc     1920

gtgttgtgtc aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc cagccatgat     1980

ggcggtaagc aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat     2040

ggactgacac ccgaacaggt ggtcgccatt gctaataata acggaggacg gccagcctt      2099


<210>  2
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Left TALE 1755

<400>  2

Met Val Asp Leu Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys 
1               5                   10                  15      


Ile Lys Pro Lys Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu 
            20                  25                  30          


Val Gly His Gly Phe Thr His Ala His Ile Val Ala Leu Ser Gln His 
        35                  40                  45              


Pro Ala Ala Leu Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala 
    50                  55                  60                  


Ala Leu Pro Glu Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln 
65                  70                  75                  80  


Trp Ser Gly Ala Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu 
                85                  90                  95      


Leu Arg Gly Pro Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile 
            100                 105                 110         


Ala Lys Arg Gly Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg 
        115                 120                 125             


Asn Ala Leu Thr Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val 
    130                 135                 140                 


Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
145                 150                 155                 160 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Glu Gln 
                165                 170                 175     


Val Val Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
            180                 185                 190         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        195                 200                 205             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    210                 215                 220                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
225                 230                 235                 240 


Thr Pro Ala Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
                245                 250                 255     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            260                 265                 270         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Asn Asn Asn Gly Gly 
        275                 280                 285             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    290                 295                 300                 


Asp His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile 
305                 310                 315                 320 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                325                 330                 335     


Cys Gln Ala His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            340                 345                 350         


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        355                 360                 365             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Ala Gln Val Val Ala Ile 
    370                 375                 380                 


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
385                 390                 395                 400 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                405                 410                 415     


Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            420                 425                 430         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Glu Gln 
        435                 440                 445             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
    450                 455                 460                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
465                 470                 475                 480 


Asp Gln Val Val Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu 
                485                 490                 495     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            500                 505                 510         


Thr Pro Ala Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
        515                 520                 525             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    530                 535                 540                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Asn Asn Asn Gly Gly 
545                 550                 555                 560 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                565                 570                 575     


Asp His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp 
            580                 585                 590         


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
        595                 600                 605             


Cys Gln Ala His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
    610                 615                 620                 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
625                 630                 635                 640 


Val Leu Cys Gln Ala His Gly Leu Thr Pro Ala Gln Val Val Ala Ile 
                645                 650                 655     


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
            660                 665                 670         


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Glu Gln Val Val 
        675                 680                 685             


Ala Ile Ala Asn Asn Asn Gly Gly Arg Pro Ala 
    690                 695                 


<210>  3
<211>  2099
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right TALE 1756

<400>  3
atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat caagcctaag       60

gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt cactcatgcg      120

catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt caaataccaa      180

gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag      240

tggtcgggag cgcgagcact tgaggcgctg ctgactgtgg cgggtgagct tagggggcct      300

ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg agtaacagcg      360

gtagaggcag tgcacgcctg gcgcaatgcg ctcaccgggg cccccttgaa cctgacccca      420

gaccaggtag tcgcaatcgc gaacaataat gggggaaagc aagccctgga aaccgtgcaa      480

aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt      540

gcaaataata acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc      600

tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtcgca tgacggaggg      660

aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg      720

acgcctgcac aagtggtcgc catcgcctcc aatattggcg gtaagcaggc gctggaaaca      780

gtacagcgcc tgctgcctgt actgtgccag gatcatggac tgaccccaga ccaggtagtc      840

gcaatcgcga acaataatgg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg      900

gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aaataataac      960

ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac     1020

gggctgactc ccgatcaagt tgtagcgatt gcgtcgcatg acggagggaa acaagcattg     1080

gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa     1140

gtggtcgcca tcgccaacaa caacggcggt aagcaggcgc tggaaacagt acagcgcctg     1200

ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc aatcgcgaac     1260

aataatgggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa     1320

gaccacggcc ttacaccgga gcaagtcgtg gccattgcaa gcaatggggg tggcaaacag     1380

gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc     1440

gatcaagttg tagcgattgc gaataacaat ggagggaaac aagcattgga gactgtccaa     1500

cggctccttc ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc     1560

gccaacaaca acggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg     1620

tgccaggatc atggactgac cccagaccag gtagtcgcaa tcgcgaacaa taatggggga     1680

aagcaagccc tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt     1740

acaccggagc aagtcgtggc cattgcatcc cacgacggtg gcaaacaggc tcttgagacg     1800

gttcagagac ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta     1860

gcgattgcga ataacaatgg agggaaacaa gcattggaga ctgtccaacg gctccttccc     1920

gtgttgtgtc aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc cagccatgat     1980

ggcggtaagc aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat     2040

ggactgacac ccgaacaggt ggtcgccatt gctaataata acggaggacg gccagcctt      2099


<210>  4
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Right TALE 1756

<400>  4

Met Val Asp Leu Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys 
1               5                   10                  15      


Ile Lys Pro Lys Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu 
            20                  25                  30          


Val Gly His Gly Phe Thr His Ala His Ile Val Ala Leu Ser Gln His 
        35                  40                  45              


Pro Ala Ala Leu Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala 
    50                  55                  60                  


Ala Leu Pro Glu Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln 
65                  70                  75                  80  


Trp Ser Gly Ala Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu 
                85                  90                  95      


Leu Arg Gly Pro Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile 
            100                 105                 110         


Ala Lys Arg Gly Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg 
        115                 120                 125             


Asn Ala Leu Thr Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val 
    130                 135                 140                 


Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
145                 150                 155                 160 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Glu Gln 
                165                 170                 175     


Val Val Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
            180                 185                 190         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        195                 200                 205             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    210                 215                 220                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
225                 230                 235                 240 


Thr Pro Ala Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
                245                 250                 255     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            260                 265                 270         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Asn Asn Asn Gly Gly 
        275                 280                 285             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    290                 295                 300                 


Asp His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Asn Asn Asn 
305                 310                 315                 320 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                325                 330                 335     


Cys Gln Ala His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            340                 345                 350         


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        355                 360                 365             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Ala Gln Val Val Ala Ile 
    370                 375                 380                 


Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
385                 390                 395                 400 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                405                 410                 415     


Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            420                 425                 430         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Glu Gln 
        435                 440                 445             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    450                 455                 460                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
465                 470                 475                 480 


Asp Gln Val Val Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln Ala Leu 
                485                 490                 495     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            500                 505                 510         


Thr Pro Ala Gln Val Val Ala Ile Ala Asn Asn Asn Gly Gly Lys Gln 
        515                 520                 525             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    530                 535                 540                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Asn Asn Asn Gly Gly 
545                 550                 555                 560 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                565                 570                 575     


Asp His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp 
            580                 585                 590         


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
        595                 600                 605             


Cys Gln Ala His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Asn 
    610                 615                 620                 


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
625                 630                 635                 640 


Val Leu Cys Gln Ala His Gly Leu Thr Pro Ala Gln Val Val Ala Ile 
                645                 650                 655     


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
            660                 665                 670         


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Glu Gln Val Val 
        675                 680                 685             


Ala Ile Ala Asn Asn Asn Gly Gly Arg Pro Ala 
    690                 695                 


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left Target

<400>  5
tggaagactg agtgcccg                                                     18


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right Target

<400>  6
tggcaggcgg tgggcgcg                                                     18


<210>  7
<211>  4420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Donor molecule

<400>  7
ccgccctcgg tgtccccaca ggatgaaaca gtaagttggt ggaggggagg gggtccgtca       60

gggacaattg ggagagaaaa ggtgagggct tcccgggtgg cgtgcactgt agagccctct      120

agggacttcc tgaacagaag cagacagaaa ccacggagag acgaggttac ttcagacatg      180

ggacggtctc tgtagttaca gtggggcatt aagtaagggt gtgtgtgttg ctggggatct      240

gagaagtcga tctttgagct gagcgctggt gaaggagaaa caagccatgg aaggaaaggt      300

gccaagtggt caggcgagag cctccagggc aaaggccttg ggcaggtggg aatcctgatt      360

tgttcctgaa aggtagtttg gctgaatcat tcctgagaag gctggagagg ccagcaggaa      420

acaaaaccca gcaaggcctt ttgtcgtgag ggcattaggg agctggaggg attttgagca      480

gcagagggac ataggttgtg ttagtgtttg agcaccagcc ctctggtccc tgtgtagatt      540

tagaggacca gactcaggga tggggctgag ggaggtaggg aagggagggg gcttggatca      600

ttgcaggagc tatggggatt ccagaaatgt tgaggggacg gaggagtagg ggataaacaa      660

ggattcctag cctggaacca gtgcccaagt cctgagtctt ccaggagcca caggcagcct      720

taagcctggt ccccatacac aggctgaagt ggcagttcca gcggctgtcc ctgcggcaga      780

ggctgaggcc gaggtgacgc tgcgggagct ccaggaagcc ctggaggagg aggtgctcac      840

ccggcagagc ctgagccggg agatggaggc catccgcacg gacaaccaga acttcgccag      900

gtcgggatcg gggccggggc cggggccggg atgcgggccg gtggcaaccc ttggcatccc      960

ctctcgtccg gcccggacgg actcaccgtc cttacctccc cacagtcaac tacgcgaggc     1020

agaggctcgg aaccgggacc tagaggcaca cgtccggcag ttgcaggagc ggatggagtt     1080

gctgcaggca gagggagcca caggtgagtc cctcatgtgt ccccttcccc ggaggaccgg     1140

gaggaggtgg gccgtctgct ccgcggggcg tgtatagaca cctggaggag ggaagggacc     1200

cacgctgggg cacgccgcgc caccgccctc cttcgcccct ccacgcgccc tatgcctctt     1260

tcttctcctt ccagctgtca cgggggtccc cagtccccgg gccacggatc caccttccca     1320

tgtaagaccc ctctctttcc cctgcctcag acctgctgcc cattctgcag atcccctccc     1380

tggctcctgg tctccccgtc cagatatagg gctcacccta cgtctttgcg actttagagg     1440

gcagaagccc tttattcagc cccagatctc cctccgttca ggcctcacca gattccctcc     1500

gggatctccc tagataacct ccccaacctc gattcccctc gctgtctctc gccccaccgc     1560

tgagggctgg gctgggctcc gatcgggtca cctgtccctt ctctctccag ctagatggcc     1620

ccccggccgt ggctgtgggc cagtgcccgc tggtggggcc aggccccatg caccgccgcc     1680

acctgctgct ccctgccagg gtacgtccgg ctgcccacgc ccccctccgc cgtcgcgccc     1740

cgcgctccac ccgccccttg ccacccgctt agctgcgcat ttgcggggct gggcccacgg     1800

caggagggcg gatcttcggg cagccaatca acacaggccg ctaggaagca gccaatgacg     1860

agttcggacg ggattcgagg cgtgcgagtg gactaacaac agctgtaggc tgttggggcg     1920

ggggcggggc gcagggaaga gtgcgggccc acctatgggc gtaggcgggg cgagtcccag     1980

gagccaatca gaggcccatg ccgggtgttg acctcgccct ctccccgcag gtccctaggc     2040

ctggcctatc ggaggcgctt tccctgctcc tgttcgccgt tgttctgtct cgtgccgccg     2100

ccctgggctg cattgggttg gtggcccacg ccggccaact caccgcagtc tggcgccgcc     2160

caggagccgc ccgcgctccc tgaaccctag aactgtcttc gactccgggg ccccgttgga     2220

agactgagtg cccggggcac ggcatggccc aacttgttta ttgcagctta taatggttac     2280

aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt     2340

tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctcctg cagataactt     2400

cgtatagcat acattatacg aagttatatt aagggttccg gatctcgacc agcttctgat     2460

ggaattagaa cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg     2520

atctcgttct tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa     2580

agtgggaatc aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga     2640

gtgggaggaa tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact     2700

atgaaacatt atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta     2760

atcccacccc acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg     2820

ttctcaccct tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat     2880

tgtttaatag atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc     2940

gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc     3000

gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc     3060

cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc     3120

cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct     3180

ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg     3240

gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca     3300

tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga     3360

ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg     3420

tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt     3480

ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt     3540

cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttcagctgct     3600

cgagatctag atggatgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg     3660

cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg     3720

ccccgcccct gagcccgccc ctgagcccgc ccccggaccc acccttccca gctgctgagc     3780

ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca     3840

ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca     3900

cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga     3960

aggtggcgcg aaggggccac caaagaacgg agccggttgg cgctaccggt ggatgtggaa     4020

tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc cagcggggct gctaaagcgc     4080

atgctccaga ctgccttggg aaaagcgcct cccctacccg gtagaatttc gaggtcgaga     4140

tcctaagctt ggctggacgt aaactcctct tcagacctaa taacttcgta tagcatacat     4200

tatacgaagt tatgtcgacg cacagaagcc gcgcccaccg cctgccagtt cacaaccgct     4260

ccgagcgtgg gtctccgccc agctccagtc ctgtgatccg ggcccgcccc ctagcggccg     4320

gggagggagg ggccgggtcc gcggccggcg aacggggctc gaagggtcct tgtagccggg     4380

aatgctgctg ctgctgctgc tgctgctgct gctgctgctg                           4420


<210>  8
<211>  2242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left homology

<400>  8
ccgccctcgg tgtccccaca ggatgaaaca gtaagttggt ggaggggagg gggtccgtca       60

gggacaattg ggagagaaaa ggtgagggct tcccgggtgg cgtgcactgt agagccctct      120

agggacttcc tgaacagaag cagacagaaa ccacggagag acgaggttac ttcagacatg      180

ggacggtctc tgtagttaca gtggggcatt aagtaagggt gtgtgtgttg ctggggatct      240

gagaagtcga tctttgagct gagcgctggt gaaggagaaa caagccatgg aaggaaaggt      300

gccaagtggt caggcgagag cctccagggc aaaggccttg ggcaggtggg aatcctgatt      360

tgttcctgaa aggtagtttg gctgaatcat tcctgagaag gctggagagg ccagcaggaa      420

acaaaaccca gcaaggcctt ttgtcgtgag ggcattaggg agctggaggg attttgagca      480

gcagagggac ataggttgtg ttagtgtttg agcaccagcc ctctggtccc tgtgtagatt      540

tagaggacca gactcaggga tggggctgag ggaggtaggg aagggagggg gcttggatca      600

ttgcaggagc tatggggatt ccagaaatgt tgaggggacg gaggagtagg ggataaacaa      660

ggattcctag cctggaacca gtgcccaagt cctgagtctt ccaggagcca caggcagcct      720

taagcctggt ccccatacac aggctgaagt ggcagttcca gcggctgtcc ctgcggcaga      780

ggctgaggcc gaggtgacgc tgcgggagct ccaggaagcc ctggaggagg aggtgctcac      840

ccggcagagc ctgagccggg agatggaggc catccgcacg gacaaccaga acttcgccag      900

gtcgggatcg gggccggggc cggggccggg atgcgggccg gtggcaaccc ttggcatccc      960

ctctcgtccg gcccggacgg actcaccgtc cttacctccc cacagtcaac tacgcgaggc     1020

agaggctcgg aaccgggacc tagaggcaca cgtccggcag ttgcaggagc ggatggagtt     1080

gctgcaggca gagggagcca caggtgagtc cctcatgtgt ccccttcccc ggaggaccgg     1140

gaggaggtgg gccgtctgct ccgcggggcg tgtatagaca cctggaggag ggaagggacc     1200

cacgctgggg cacgccgcgc caccgccctc cttcgcccct ccacgcgccc tatgcctctt     1260

tcttctcctt ccagctgtca cgggggtccc cagtccccgg gccacggatc caccttccca     1320

tgtaagaccc ctctctttcc cctgcctcag acctgctgcc cattctgcag atcccctccc     1380

tggctcctgg tctccccgtc cagatatagg gctcacccta cgtctttgcg actttagagg     1440

gcagaagccc tttattcagc cccagatctc cctccgttca ggcctcacca gattccctcc     1500

gggatctccc tagataacct ccccaacctc gattcccctc gctgtctctc gccccaccgc     1560

tgagggctgg gctgggctcc gatcgggtca cctgtccctt ctctctccag ctagatggcc     1620

ccccggccgt ggctgtgggc cagtgcccgc tggtggggcc aggccccatg caccgccgcc     1680

acctgctgct ccctgccagg gtacgtccgg ctgcccacgc ccccctccgc cgtcgcgccc     1740

cgcgctccac ccgccccttg ccacccgctt agctgcgcat ttgcggggct gggcccacgg     1800

caggagggcg gatcttcggg cagccaatca acacaggccg ctaggaagca gccaatgacg     1860

agttcggacg ggattcgagg cgtgcgagtg gactaacaac agctgtaggc tgttggggcg     1920

ggggcggggc gcagggaaga gtgcgggccc acctatgggc gtaggcgggg cgagtcccag     1980

gagccaatca gaggcccatg ccgggtgttg acctcgccct ctccccgcag gtccctaggc     2040

ctggcctatc ggaggcgctt tccctgctcc tgttcgccgt tgttctgtct cgtgccgccg     2100

ccctgggctg cattgggttg gtggcccacg ccggccaact caccgcagtc tggcgccgcc     2160

caggagccgc ccgcgctccc tgaaccctag aactgtcttc gactccgggg ccccgttgga     2220

agactgagtg cccggggcac gg                                              2242


<210>  9
<211>  202
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right homology

<400>  9
cgcacagaag ccgcgcccac cgcctgccag ttcacaaccg ctccgagcgt gggtctccgc       60

ccagctccag tcctgtgatc cgggcccgcc ccctagcggc cggggaggga ggggccgggt      120

ccgcggccgg cgaacggggc tcgaagggtc cttgtagccg ggaatgctgc tgctgctgct      180

gctgctgctg ctgctgctgc tg                                               202


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  10
tttcggccag gctgaggc                                                     18


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  11
ttcccaggcc tgcagttt                                                     18


<210>  12
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  12
tccgagcgtg ggtctccg                                                     18


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  13
tagggggcgg gcccggat                                                     18


<210>  14
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  14
tccagggcct ggacagg                                                      17


<210>  15
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  15
tcggggtcct cctgtc                                                       16


<210>  16
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  16
tggtgctgcc tgtccaa                                                      17


<210>  17
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  17
tggagccgcc tcagccg                                                      17


<210>  18
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  18
tgtgaggggt taaggctg                                                     18


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  19
tccccacccc ttggtcca                                                     18


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  20
tttcggccag gctgaggc                                                     18


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  21
ttcccaggcc tgcagttt                                                     18


<210>  22
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  22
tccgagcgtg ggtctccg                                                     18


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  23
tagggggcgg gcccggat                                                     18


<210>  24
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left target

<400>  24
tccagggcct ggacagg                                                      17


<210>  25
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right target

<400>  25
tcggggtcct cctgtc                                                       16


<210>  26
<211>  9159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left TALEN expression plasmid

<400>  26
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat      960

gacgatgaca agatggcccc caagaagaag aggaaggtgg gcattcaccg cggggtacct     1020

atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat caagcctaag     1080

gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt cactcatgcg     1140

catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt caaataccaa     1200

gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag     1260

tggtcgggag cgcgagcact tgaggcgctg ctgactgtgg cgggtgagct tagggggcct     1320

ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg agtaacagcg     1380

gtagaggcag tgcacgcctg gcgcaatgcg ctcaccgggg cccccttgaa cagagacgat     1440

taaatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa aatcaagcct     1500

aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg cttcactcat     1560

gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc tgtcaaatac     1620

caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg ggtcggtaaa     1680

cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga gcttaggggg     1740

cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg gggagtaaca     1800

gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gggccccctt gaacctgacc     1860

ccagaccagg tagtcgcaat cgcgaacaat aatgggggaa agcaagccct ggaaaccgtg     1920

caaaggttgt tgccggtcct ttgtcaagac cacggcctta caccggagca agtcgtggcc     1980

attgcaaata ataacggtgg caaacaggct cttgagacgg ttcagagact tctcccagtt     2040

ctctgtcaag cccacgggct gactcccgat caagttgtag cgattgcgtc gcatgacgga     2100

gggaaacaag cattggagac tgtccaacgg ctccttcccg tgttgtgtca agcccacggt     2160

ttgacgcctg cacaagtggt cgccatcgcc tccaatattg gcggtaagca ggcgctggaa     2220

acagtacagc gcctgctgcc tgtactgtgc caggatcatg gactgacccc agaccaggta     2280

gtcgcaatcg cgaacaataa tgggggaaag caagccctgg aaaccgtgca aaggttgttg     2340

ccggtccttt gtcaagacca cggccttaca ccggagcaag tcgtggccat tgcaaataat     2400

aacggtggca aacaggctct tgagacggtt cagagacttc tcccagttct ctgtcaagcc     2460

cacgggctga ctcccgatca agttgtagcg attgcgtcgc atgacggagg gaaacaagca     2520

ttggagactg tccaacggct ccttcccgtg ttgtgtcaag cccacggttt gacgcctgca     2580

caagtggtcg ccatcgccaa caacaacggc ggtaagcagg cgctggaaac agtacagcgc     2640

ctgctgcctg tactgtgcca ggatcatgga ctgaccccag accaggtagt cgcaatcgcg     2700

aacaataatg ggggaaagca agccctggaa accgtgcaaa ggttgttgcc ggtcctttgt     2760

caagaccacg gccttacacc ggagcaagtc gtggccattg caagcaatgg gggtggcaaa     2820

caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca cgggctgact     2880

cccgatcaag ttgtagcgat tgcgaataac aatggaggga aacaagcatt ggagactgtc     2940

caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca agtggtcgcc     3000

atcgccaaca acaacggcgg taagcaggcg ctggaaacag tacagcgcct gctgcctgta     3060

ctgtgccagg atcatggact gaccccagac caggtagtcg caatcgcgaa caataatggg     3120

ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg tcctttgtca agaccacggc     3180

cttacaccgg agcaagtcgt ggccattgca tcccacgacg gtggcaaaca ggctcttgag     3240

acggttcaga gacttctccc agttctctgt caagcccacg ggctgactcc cgatcaagtt     3300

gtagcgattg cgaataacaa tggagggaaa caagcattgg agactgtcca acggctcctt     3360

cccgtgttgt gtcaagccca cggtttgacg cctgcacaag tggtcgccat cgccagccat     3420

gatggcggta agcaggcgct ggaaacagta cagcgcctgc tgcctgtact gtgccaggat     3480

catggactga cacccgaaca ggtggtcgcc attgctaata ataacggagg acggccagcc     3540

ttggagtcca tcgtagccca attgtccagg cccgatcccg cgttggctgc gttaacgaat     3600

gaccatctgg tggcgttggc atgtcttggt ggacgacccg cgctcgatgc agtcaaaaag     3660

ggtctgcctc atgctcccgc attgatcaaa agaaccaacc ggcggattcc cgagagaact     3720

tcccatcgag tcgcgggatc ccaactagtc aaaagtgaac tggaggagaa gaaatctgaa     3780

cttcgtcata aattgaaata tgtgcctcat gaatatattg aattaattga aattgccaga     3840

aattccactc aggatagaat tcttgaaatg aaggtaatgg aattttttat gaaagtttat     3900

ggatatagag gtaaacattt gggtggatca aggaaaccgg acggagcaat ttatactgtc     3960

ggatctccta ttgattacgg tgtgatcgtg gatactaaag cttatagcgg aggttataat     4020

ctgccaattg gccaagcaga tgaaatgcaa cgatatgtcg aagaaaatca aacacgaaac     4080

aaacatatca accctaatga atggtggaaa gtctatccat cttctgtaac ggaatttaag     4140

tttttatttg tgagtggtca ctttaaagga aactacaaag ctcagcttac acgattaaat     4200

catatcacta attgtaatgg agctgttctt agtgtagaag agcttttaat tggtggagaa     4260

atgattaaag ccggcacatt aaccttagag gaagtcagac ggaaatttaa taacggcgag     4320

ataaacttta ggtccggcgg cggagagggc agaggaagtc ttctaacatg cggtgacgtg     4380

gaggagaatc ccggcccaat gcccgccatg aagatcgagt gccgcatcac cggcaccctg     4440

aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccgagca gggccgcatg     4500

accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac     4560

gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc     4620

ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc     4680

ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc     4740

aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc     4800

agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc     4860

gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg     4920

cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc     4980

cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc     5040

ttcaagaccc ccatcgcctt cgcctaaacc ggtcatcatc accatcacca ttgagtttaa     5100

acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     5160

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     5220

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     5280

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     5340

atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt     5400

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     5460

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc     5520

tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg     5580

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     5640

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     5700

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     5760

gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa     5820

ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga     5880

agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc     5940

ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc     6000

ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc     6060

tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag     6120

aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt     6180

atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg     6240

gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag     6300

aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact     6360

acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat     6420

atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg     6480

cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc     6540

cctgcggacg gtgtcgacag gtgcttctcg atctgcatcc tgggatcaaa gcgatagtga     6600

aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg     6660

tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc     6720

gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc     6780

tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt     6840

attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     6900

tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     6960

tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg     7020

tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa     7080

gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct     7140

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga     7200

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc     7260

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     7320

tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt     7380

aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa     7440

aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt     7500

ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg     7560

tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc     7620

agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc     7680

gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta     7740

tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct     7800

acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc     7860

tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa     7920

caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa     7980

aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa     8040

aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt     8100

ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac     8160

agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc     8220

atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc     8280

cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata     8340

aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc     8400

cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc     8460

aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca     8520

ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa     8580

gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca     8640

ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt     8700

tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt     8760

tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg     8820

ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga     8880

tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc     8940

agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg     9000

acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag     9060

ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     9120

gttccgcgca catttccccg aaaagtgcca cctgacgtc                            9159


<210>  27
<211>  9191
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right TALEN expression plasmid

<400>  27
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat      960

gacgatgaca agatggcccc caagaagaag aggaaggtgg gcattcaccg cggggtacct     1020

atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat caagcctaag     1080

gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt cactcatgcg     1140

catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt caaataccaa     1200

gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag     1260

tggtcgggag cgcgagcact tgaggcgctg ctgactgtgg cgggtgagct tagggggcct     1320

ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg agtaacagcg     1380

gtagaggcag tgcacgcctg gcgcaatgcg ctcaccgggg cccccttgaa cagagacgat     1440

taaatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa aatcaagcct     1500

aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg cttcactcat     1560

gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc tgtcaaatac     1620

caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg ggtcggtaaa     1680

cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga gcttaggggg     1740

cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg gggagtaaca     1800

gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gggccccctt gaacctgacc     1860

ccagaccagg tagtcgcaat cgcgaacaat aatgggggaa agcaagccct ggaaaccgtg     1920

caaaggttgt tgccggtcct ttgtcaagac cacggcctta caccggagca agtcgtggcc     1980

attgcaaata ataacggtgg caaacaggct cttgagacgg ttcagagact tctcccagtt     2040

ctctgtcaag cccacgggct gactcccgat caagttgtag cgattgcgtc gaacattgga     2100

gggaaacaag cattggagac tgtccaacgg ctccttcccg tgttgtgtca agcccacggt     2160

ttgacgcctg cacaagtggt cgccatcgcc tccaatattg gcggtaagca ggcgctggaa     2220

acagtacagc gcctgctgcc tgtactgtgc caggatcatg gactgacccc agaccaggta     2280

gtcgcaatcg cgaacaataa tgggggaaag caagccctgg aaaccgtgca aaggttgttg     2340

ccggtccttt gtcaagacca cggccttaca ccggagcaag tcgtggccat tgcaagcaac     2400

atcggtggca aacaggctct tgagacggtt cagagacttc tcccagttct ctgtcaagcc     2460

cacgggctga ctcccgatca agttgtagcg attgcgtcgc atgacggagg gaaacaagca     2520

ttggagactg tccaacggct ccttcccgtg ttgtgtcaag cccacggttt gacgcctgca     2580

caagtggtcg ccatcgcctc gaatggcggc ggtaagcagg cgctggaaac agtacagcgc     2640

ctgctgcctg tactgtgcca ggatcatgga ctgaccccag accaggtagt cgcaatcgcg     2700

aacaataatg ggggaaagca agccctggaa accgtgcaaa ggttgttgcc ggtcctttgt     2760

caagaccacg gccttacacc ggagcaagtc gtggccattg caagcaacat cggtggcaaa     2820

caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca cgggctgact     2880

cccgatcaag ttgtagcgat tgcgaataac aatggaggga aacaagcatt ggagactgtc     2940

caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca agtggtcgcc     3000

atcgcctcga atggcggcgg taagcaggcg ctggaaacag tacagcgcct gctgcctgta     3060

ctgtgccagg atcatggact gaccccagac caggtagtcg caatcgcgaa caataatggg     3120

ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg tcctttgtca agaccacggc     3180

cttacaccgg agcaagtcgt ggccattgca tcccacgacg gtggcaaaca ggctcttgag     3240

acggttcaga gacttctccc agttctctgt caagcccacg ggctgactcc cgatcaagtt     3300

gtagcgattg cgtcgcatga cggagggaaa caagcattgg agactgtcca acggctcctt     3360

cccgtgttgt gtcaagccca cggtttgacg cctgcacaag tggtcgccat cgccagccat     3420

gatggcggta agcaggcgct ggaaacagta cagcgcctgc tgcctgtact gtgccaggat     3480

catggactga cacccgaaca ggtggtcgcc attgctaata ataacggagg acggccagcc     3540

ttggagtcca tcgtagccca attgtccagg cccgatcccg cgttggctgc gttaacgaat     3600

gaccatctgg tggcgttggc atgtcttggt ggacgacccg cgctcgatgc agtcaaaaag     3660

ggtctgcctc atgctcccgc attgatcaaa agaaccaacc ggcggattcc cgagagaact     3720

tcccatcgag tcgcgggatc ccaactagtc aaaagtgaac tggaggagaa gaaatctgaa     3780

cttcgtcata aattgaaata tgtgcctcat gaatatattg aattaattga aattgccaga     3840

aattccactc aggatagaat tcttgaaatg aaggtaatgg aattttttat gaaagtttat     3900

ggatatagag gtaaacattt gggtggatca aggaaaccgg acggagcaat ttatactgtc     3960

ggatctccta ttgattacgg tgtgatcgtg gatactaaag cttatagcgg aggttataat     4020

ctgccaattg gccaagcaga tgaaatgcaa cgatatgtcg aagaaaatca aacacgaaac     4080

aaacatatca accctaatga atggtggaaa gtctatccat cttctgtaac ggaatttaag     4140

tttttatttg tgagtggtca ctttaaagga aactacaaag ctcagcttac acgattaaat     4200

catatcacta attgtaatgg agctgttctt agtgtagaag agcttttaat tggtggagaa     4260

atgattaaag ccggcacatt aaccttagag gaagtcagac ggaaatttaa taacggcgag     4320

ataaacttta ggtccggcgg cggagagggc agaggaagtc ttctaacatg cggtgacgtg     4380

gaggagaatc ccggcccaat gagcgagctg attaaggaga acatgcacat gaagctgtac     4440

atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc     4500

tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc     4560

ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag     4620

ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc     4680

acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc     4740

ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag     4800

aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg     4860

gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc     4920

aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat     4980

gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac     5040

gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattaa     5100

ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt     5160

ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg     5220

ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt     5280

gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca     5340

atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct     5400

ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     5460

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     5520

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca     5580

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     5640

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     5700

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     5760

cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg     5820

agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg     5880

tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt     5940

cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     6000

atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     6060

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     6120

ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     6180

taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg     6240

tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa     6300

ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc     6360

tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag     6420

cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga     6480

actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc     6540

gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgtcgac aggtgcttct     6600

cgatctgcat cctgggatca aagcgatagt gaaggacagt gatggacagc cgacggcagt     6660

tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg     6720

aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt     6780

tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca     6840

tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa     6900

gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt     6960

tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct     7020

tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac     7080

acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac     7140

tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc     7200

tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg     7260

cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc     7320

actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt     7380

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     7440

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     7500

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     7560

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     7620

cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     7680

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     7740

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     7800

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     7860

acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg     7920

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt     7980

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     8040

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga     8100

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa     8160

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac     8220

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga     8280

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc     8340

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca     8400

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta     8460

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg     8520

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc     8580

gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg     8640

ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt     8700

ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt     8760

cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata     8820

ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc     8880

gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac     8940

ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa     9000

ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct     9060

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat     9120

ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc     9180

cacctgacgt c                                                          9191


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
ggtgtgatcg tggatactaa agc                                               23


<210>  29
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
tgggccggga ttctcctcca cgtcaccgca tgttagaaga cttcctctgc cctctccgcc       60

gccggaccta aagtttatct cgccgttatt aaat                                   94


<210>  30
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
atgcggtgac gtggaggaga atcccggccc aatgcccgcc atgaagatcg ag               52


<210>  31
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
ctcaatggtg atggtgatga tgaccggttt aggcgaaggc gatgggggtc                  50


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
ctgacttcct ctaaggttaa t                                                 21


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
ggcaactaga aggcacagtc                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
ccggcggatt cccgagagaa                                                   20


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
cagctcgctc attgggccgg gatt                                              24


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
cccggcccaa tgagcgagct gatt                                              24


<210>  37
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
cccgaccggt taattaagct tgtgccc                                           27


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
ctgacttcct ctaaggttaa t                                                 21


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
ggcaactaga aggcacagtc                                                   20


<210>  40
<211>  6789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt      180

gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt      240

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg      300

acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca      360

aggcctaggc gcgccatgag ctccgccctc ggtgtcccca caggatgaaa cagtaagttg      420

gtggagggga gggggtccgt cagggacaat tgggagagaa aaggtgaggg cttcccgggt      480

ggcgtgcact gtagagccct ctagggactt cctgaacaga agcagacaga aaccacggag      540

agacgaggtt acttcagaca tgggacggtc tctgtagtta cagtggggca ttaagtaagg      600

gtgtgtgtgt tgctggggat ctgagaagtc gatctttgag ctgagcgctg gtgaaggaga      660

aacaagccat ggaaggaaag gtgccaagtg gtcaggcgag agcctccagg gcaaaggcct      720

tgggcaggtg ggaatcctga tttgttcctg aaaggtagtt tggctgaatc attcctgaga      780

aggctggaga ggccagcagg aaacaaaacc cagcaaggcc ttttgtcgtg agggcattag      840

ggagctggag ggattttgag cagcagaggg acataggttg tgttagtgtt tgagcaccag      900

ccctctggtc cctgtgtaga tttagaggac cagactcagg gatggggctg agggaggtag      960

ggaagggagg gggcttggat cattgcagga gctatgggga ttccagaaat gttgagggga     1020

cggaggagta ggggataaac aaggattcct agcctggaac cagtgcccaa gtcctgagtc     1080

ttccaggagc cacaggcagc cttaagcctg gtccccatac acaggctgaa gtggcagttc     1140

cagcggctgt ccctgcggca gaggctgagg ccgaggtgac gctgcgggag ctccaggaag     1200

ccctggagga ggaggtgctc acccggcaga gcctgagccg ggagatggag gccatccgca     1260

cggacaacca gaacttcgcc aggtcgggat cggggccggg gccggggccg ggatgcgggc     1320

cggtggcaac ccttggcatc ccctctcgtc cggcccggac ggactcaccg tccttacctc     1380

cccacagtca actacgcgag gcagaggctc ggaaccggga cctagaggca cacgtccggc     1440

agttgcagga gcggatggag ttgctgcagg cagagggagc cacaggtgag tccctcatgt     1500

gtccccttcc ccggaggacc gggaggaggt gggccgtctg ctccgcgggg cgtgtataga     1560

cacctggagg agggaaggga cccacgctgg ggcacgccgc gccaccgccc tccttcgccc     1620

ctccacgcgc cctatgcctc tttcttctcc ttccagctgt cacgggggtc cccagtcccc     1680

gggccacgga tccaccttcc catgtaagac ccctctcttt cccctgcctc agacctgctg     1740

cccattctgc agatcccctc cctggctcct ggtctccccg tccagatata gggctcaccc     1800

tacgtctttg cgactttaga gggcagaagc cctttattca gccccagatc tccctccgtt     1860

caggcctcac cagattccct ccgggatctc cctagataac ctccccaacc tcgattcccc     1920

tcgctgtctc tcgccccacc gctgagggct gggctgggct ccgatcgggt cacctgtccc     1980

ttctctctcc agctagatgg ccccccggcc gtggctgtgg gccagtgccc gctggtgggg     2040

ccaggcccca tgcaccgccg ccacctgctg ctccctgcca gggtacgtcc ggctgcccac     2100

gcccccctcc gccgtcgcgc cccgcgctcc acccgcccct tgccacccgc ttagctgcgc     2160

atttgcgggg ctgggcccac ggcaggaggg cggatcttcg ggcagccaat caacacaggc     2220

cgctaggaag cagccaatga cgagttcgga cgggattcga ggcgtgcgag tggactaaca     2280

acagctgtag gctgttgggg cgggggcggg gcgcagggaa gagtgcgggc ccacctatgg     2340

gcgtaggcgg ggcgagtccc aggagccaat cagaggccca tgccgggtgt tgacctcgcc     2400

ctctccccgc aggtccctag gcctggccta tcggaggcgc tttccctgct cctgttcgcc     2460

gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt tggtggccca cgccggccaa     2520

ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc cctgaaccct agaactgtct     2580

tcgactccgg ggccccgttg gaagactgag tgcccggggc acggcatggc ccaacttgtt     2640

tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc     2700

atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt     2760

ctggatctcc tgcagataac ttcgtatagc atacattata cgaagttata ttaagggttc     2820

cggatctcga ccagcttctg atggaattag aacttggcaa aacaatactg agaatgaagt     2880

gtatgtggaa cagaggctgc tgatctcgtt cttcaggcta tgaaactgac acatttggaa     2940

accacagtac ttagaaccac aaagtgggaa tcaagagaaa aacaatgatc ccacgagaga     3000

tctatagatc tatagatcat gagtgggagg aatgagctgg cccttaattt ggttttgctt     3060

gtttaaatta tgatatccaa ctatgaaaca ttatcataaa gcaatagtaa agagccttca     3120

gtaaagagca ggcatttatc taatcccacc ccacccccac ccccgtagct ccaatccttc     3180

cattcaaaat gtaggtactc tgttctcacc cttcttaaca aagtatgaca ggaaaaactt     3240

ccattttagt ggacatcttt attgtttaat agatcatcaa tttctgcatc ccggggatct     3300

gatatcatcg atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc     3360

gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg     3420

acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag     3480

gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg     3540

gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac     3600

gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc     3660

cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg     3720

acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg     3780

ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg     3840

tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg     3900

gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg     3960

gtcatggtaa gcttcagctg ctcgagatct agatggatgc aggtcgaaag gcccggagat     4020

gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct     4080

ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac     4140

ccacccttcc cagctgctga gcccagaaag cgaaggagca aagctgctat tggccgctgc     4200

cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga     4260

gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact     4320

tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt     4380

ggcgctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt     4440

gccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc     4500

cggtagaatt tcgaggtcga gatcctaagc ttggctggac gtaaactcct cttcagacct     4560

aataacttcg tatagcatac attatacgaa gttatgtcga cgcacagaag ccgcgcccac     4620

cgcctgccag ttcacaaccg ctccgagcgt gggtctccgc ccagctccag tcctgtgatc     4680

cgggcccgcc ccctagcggc cggggaggga ggggccgggt ccgcggccgg cgaacggggc     4740

tcgaagggtc cttgtagccg ggaatgctgc tgctgctgct gctgctgctg ctgctgctgc     4800

tgggtacctc ttaattaact ggcctcatgg gccttccgct cactgcccgc tttccagtcg     4860

ggaaacctgt cgtgccagct gcattaacat ggtcatagct gtttccttgc gtattgggcg     4920

ctctccgctt cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg     4980

cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt     5040

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt     5100

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     5160

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     5220

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     5280

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     5340

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     5400

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     5460

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta     5520

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg     5580

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt     5640

tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg     5700

tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta     5760

aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg     5820

aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg     5880

tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc     5940

gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg     6000

agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg     6060

aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag     6120

gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat     6180

caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc     6240

cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc     6300

ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa     6360

ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac     6420

gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt     6480

cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc     6540

gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa     6600

caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca     6660

tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat     6720

acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa     6780

aagtgccac                                                             6789


<210>  41
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
ggcctaggcg cgccatgagc tccgccctcg gtgtccccac aggatgaaac                  50


<210>  42
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  42
gcaataaaca agttgggcca tgccgtgccc cgggcactca gtcttccaac                  50


<210>  43
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligo comprising left and right homology (mutant)

<400>  43
gggaggggcc gggtccgcgg ccggcgaacg gggctcgaag ggtccttgta gccgggaatg       60

ctgctgctgc tgctgggggg atatcagacc atttctttct ttcggccagg ctgaggccct      120

gacgtggatg ggcaaactgc                                                  140


<210>  44
<211>  4140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  44
atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc       60

attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc      120

cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa      180

gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc      240

tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg      300

ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc      360

aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag      420

aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat      480

atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat      540

gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg      600

atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg      660

cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat      720

cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa      780

gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc      840

cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt      900

ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt      960

atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga     1020

cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc     1080

ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg     1140

gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc     1200

aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac     1260

gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt     1320

gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc     1380

agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa     1440

gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa     1500

aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt     1560

tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg     1620

tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc     1680

gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc     1740

agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc     1800

attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc     1860

ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct     1920

catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg     1980

cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg     2040

gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac     2100

tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt     2160

cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc     2220

gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt     2280

atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg     2340

atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca     2400

gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg     2460

gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat     2520

atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc     2580

gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa     2640

aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg     2700

actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag     2760

cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac     2820

accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct     2880

aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat     2940

taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa     3000

tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa     3060

atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc     3120

aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga     3180

ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc     3240

gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta     3300

cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc     3360

gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct     3420

tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc     3480

aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac     3540

tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag     3600

tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg     3660

cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc     3720

cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa     3780

caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg     3840

atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag     3900

cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg     3960

cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag     4020

gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc     4080

gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag gaaggtgtga     4140


<210>  45
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA14189 with scaffold sequence

<400>  45
tcgaagggtc cttgtagccg ttttagagct agaaatagca agttaaaata aggctagtcc       60

gttatcaact tgaaaaagtg gcaccgagtc ggtgct                                 96


<210>  46
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA14254 with scaffold sequence

<400>  46
gctgctgctg ctgctgctgc gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgct                                97


<210>  47
<211>  1001
<212>  DNA
<213>  Homo sapiens

<400>  47
tttccctgct cctgttcgcc gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt       60

tggtggccca cgccggccaa ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc      120

cctgaaccct agaactgtct tcgactccgg ggccccgttg gaagactgag tgcccggggc      180

acggcacaga agccgcgccc accgcctgcc agttcacaac cgctccgagc gtgggtctcc      240

gcccagctcc agtcctgtga tccgggcccg ccccctagcg gccggggagg gaggggccgg      300

gtccgcggcc ggcgaacggg gctcgaaggg tccttgtagc cgggaatgct gctgctgctg      360

ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctggg gggatcacag      420

accatttctt tctttcggcc aggctgaggc cctgacgtgg atgggcaaac tgcaggcctg      480

ggaaggcagc aagccgggcc gtccgtgttc catcctccac gcacccccac ctatcgttgg      540

ttcgcaaagt gcaaagcttt cttgtgcatg acgccctgct ctggggagcg tctggcgcga      600

tctctgcctg cttactcggg aaatttgctt ttgccaaacc cgctttttcg gggatcccgc      660

gcccccctcc tcacttgcgc tgctctcgga gccccagccg gctccgcccg cttcggcggt      720

ttggatattt attgacctcg tcctccgact cgctgacagg ctacaggacc cccaacaacc      780

ccaatccacg ttttggatgc actgagaccc cgacattcct cggtatttat tgtctgtccc      840

cacctaggac ccccaccccc gaccctcgcg aataaaaggc cctccatctg cccaaagctc      900

tggactccac agtgtccgcg gtttgcgttg tgggccggag gctccgcagc gggccaatcc      960

ggaggcgtgt ggaggcggcc gaaggtctgg gaggagctag c                         1001


<210>  48
<211>  421
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA14189 with scaffold sequence and U6 promoter

<400>  48
tgtacaaaaa agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc       60

gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      120

gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg      180

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      240

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      300

tggaaaggac gaaacaccgt cgaagggtcc ttgtagccgt tttagagcta gaaatagcaa      360

gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt      420

t                                                                      421


<210>  49
<211>  421
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA14254 with scaffold sequence and U6 promoter

<400>  49
tgtacaaaaa agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc       60

gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      120

gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg      180

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      240

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      300

tggaaaggac gaaacaccgc tgctgctgct gctgctgcgt tttagagcta gaaatagcaa      360

gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt      420

t                                                                      421


<210>  50
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA14189 without scaffold sequence

<400>  50
tcgaagggtc cttgtagcc                                                    19


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA14254 without scaffold sequence

<400>  51
gctgctgctg ctgctgctgc                                                   20


<210>  52
<211>  3397
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Donor molecule comprising left and right homology

<400>  52
ctccctggct cctggtctcc ccgtccagat atagggctca ccctacgtct ttgcgacttt       60

agagggcaga agccctttat tcagccccag atctccctcc gttcaggcct caccagattc      120

cctccgggat ctccctagat aacctcccca acctcgattc ccctcgctgt ctctcgcccc      180

accgctgagg gctgggctgg gctccgatcg ggtcacctgt cccttctctc tccagctaga      240

tggccccccg gccgtggctg tgggccagtg cccgctggtg gggccaggcc ccatgcaccg      300

ccgccacctg ctgctccctg ccagggtacg tccggctgcc cacgcccccc tccgccgtcg      360

cgccccgcgc tccacccgcc ccttgccacc cgcttagctg cgcatttgcg gggctgggcc      420

cacggcagga gggcggatct tcgggcagcc aatcaacaca ggccgctagg aagcagccaa      480

tgacgagttc ggacgggatt cgaggcgtgc gagtggacta acaacagctg taggctgttg      540

gggcgggggc ggggcgcagg gaagagtgcg ggcccaccta tgggcgtagg cggggcgagt      600

cccaggagcc aatcagaggc ccatgccggg tgttgacctc gccctctccc cgcaggtccc      660

taggcctggc ctatcggagg cgctttccct gctcctgttc gccgttgttc tgtctcgtgc      720

cgccgccctg ggctgcattg ggttggtggc ccacgccggc caactcaccg cagtctggcg      780

ccgcccagga gccgcccgcg ctccctgaac cctagaactg tcttcgactc cggggccccg      840

ttggaagact gagtgcccgg ggcacggcac agaagccgcg cccaccgcct gccagttcac      900

aaccgctccg agcgtgggtc tccgcccagc tccagtcctg tgatccgggc ccgcccccta      960

gcggccgggg agggaggggc cgggtccgcg gccggcgaac ggggctcgaa gggtccttgt     1020

agccggccat ggcccaactt gtttattgca gcttataatg gttacaaata aagcaatagc     1080

atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa     1140

ctcatcaatg tatcttatca tgtctggatc tctcaggcac cgggcttgcg ggtcatgcac     1200

caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg     1260

tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg     1320

gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc     1380

gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg     1440

ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg     1500

atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg     1560

gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt     1620

tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg     1680

gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg     1740

gcgaggcgca ccgtgggctt gtactcggtc atggtaagct tcagctgctc gagatctaga     1800

tggatgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc     1860

ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg     1920

agcccgcccc tgagcccgcc cccggaccca cccttcccag ctgctgagcc cagaaagcga     1980

aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg     2040

tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg     2100

acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga     2160

aggggccacc aaagaacgga gccggttggc gctaccggtg gatgtggaat gtgtgcgagg     2220

ccagaggcca cttgtgtagc gccaagtgcc agcggggctg ctaaagcgca tgctccagac     2280

tgccttggga aaagcgcctc ccctacccgg tagaatttcg aggtcgagat cctaagcttg     2340

gctggacgta aactcctctt cagacctact gctgctgggg ggatcacaga ccatttcttt     2400

ctttcggcca ggctgaggcc ctgacgtgga tgggcaaact gcaggcctgg gaaggcagca     2460

agccgggccg tccgtgttcc atcctccacg cacccccacc tatcgttggt tcgcaaagtg     2520

caaagctttc ttgtgcatga cgccctgctc tggggagcgt ctggcgcgat ctctgcctgc     2580

ttactcggga aatttgcttt tgccaaaccc gctttttcgg ggatcccgcg cccccctcct     2640

cacttgcgct gctctcggag ccccagccgg ctccgcccgc ttcggcggtt tggatattta     2700

ttgacctcgt cctccgactc gctgacaggc tacaggaccc ccaacaaccc caatccacgt     2760

tttggatgca ctgagacccc gacattcctc ggtatttatt gtctgtcccc acctaggacc     2820

cccacccccg accctcgcga ataaaaggcc ctccatctgc ccaaagctct ggactccaca     2880

gtgtccgcgg tttgcgttgt gggccggagg ctccgcagcg ggccaatccg gaggcgtgtg     2940

gaggcggccg aaggtctggg aggagctagc gggatgcgaa gcggccgaat cagggttggg     3000

ggaggaaaag ccacggggcg gggctttggc gtccggccaa taggagggcg agcgggccac     3060

ccggaggcac cgcccccgcc cagctgtggc ccagctgtgc caccgagcgt cgagaagagg     3120

gggctgggct ggcagcgcgc gcggccatcc tccttccact gcgcctgcgc acgccacgcg     3180

catccgctcc tgggacgcaa gctcgagaaa agttgctgca aactttctag cccgttcccc     3240

gcccctcctc ccggccagac ccgccccccc tgcggagccg ggaattccga ggggcggagc     3300

gcaggccgag atggggaatg tgggggcctg cagaggaccc tggagacgga ggcgtgcaga     3360

agctcagtct cggggcggag gcttcgcgcc cttagtc                              3397


<210>  53
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single stranded oligo comprising left and right homology (wild 
       type)

<400>  53
gggaggggcc gggtccgcgg ccggcgaacg gggctcgaag ggtccttgta gccgggaatg       60

ctgctgctgc tgctgggggg atcacagacc atttctttct ttcggccagg ctgaggccct      120

gacgtggatg ggcaaactgc                                                  140


<210>  54
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  left homology

<400>  54
gggaggggcc gggtccgcgg ccggcgaacg gggctcgaag ggtccttgta gccgggaatg       60


<210>  55
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right homology (mutant)

<400>  55
gggggatatc agaccatttc tttctttcgg ccaggctgag gccctgacgt ggatgggcaa       60

actgc                                                                   65


<210>  56
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right homology (wild type)

<400>  56
gggggatcac agaccatttc tttctttcgg ccaggctgag gccctgacgt ggatgggcaa       60

actgc                                                                   65


<210>  57
<211>  1026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left homology

<400>  57
ctccctggct cctggtctcc ccgtccagat atagggctca ccctacgtct ttgcgacttt       60

agagggcaga agccctttat tcagccccag atctccctcc gttcaggcct caccagattc      120

cctccgggat ctccctagat aacctcccca acctcgattc ccctcgctgt ctctcgcccc      180

accgctgagg gctgggctgg gctccgatcg ggtcacctgt cccttctctc tccagctaga      240

tggccccccg gccgtggctg tgggccagtg cccgctggtg gggccaggcc ccatgcaccg      300

ccgccacctg ctgctccctg ccagggtacg tccggctgcc cacgcccccc tccgccgtcg      360

cgccccgcgc tccacccgcc ccttgccacc cgcttagctg cgcatttgcg gggctgggcc      420

cacggcagga gggcggatct tcgggcagcc aatcaacaca ggccgctagg aagcagccaa      480

tgacgagttc ggacgggatt cgaggcgtgc gagtggacta acaacagctg taggctgttg      540

gggcgggggc ggggcgcagg gaagagtgcg ggcccaccta tgggcgtagg cggggcgagt      600

cccaggagcc aatcagaggc ccatgccggg tgttgacctc gccctctccc cgcaggtccc      660

taggcctggc ctatcggagg cgctttccct gctcctgttc gccgttgttc tgtctcgtgc      720

cgccgccctg ggctgcattg ggttggtggc ccacgccggc caactcaccg cagtctggcg      780

ccgcccagga gccgcccgcg ctccctgaac cctagaactg tcttcgactc cggggccccg      840

ttggaagact gagtgcccgg ggcacggcac agaagccgcg cccaccgcct gccagttcac      900

aaccgctccg agcgtgggtc tccgcccagc tccagtcctg tgatccgggc ccgcccccta      960

gcggccgggg agggaggggc cgggtccgcg gccggcgaac ggggctcgaa gggtccttgt     1020

agccgg                                                                1026


<210>  58
<211>  1029
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right homology

<400>  58
ctgctgctgg ggggatcaca gaccatttct ttctttcggc caggctgagg ccctgacgtg       60

gatgggcaaa ctgcaggcct gggaaggcag caagccgggc cgtccgtgtt ccatcctcca      120

cgcaccccca cctatcgttg gttcgcaaag tgcaaagctt tcttgtgcat gacgccctgc      180

tctggggagc gtctggcgcg atctctgcct gcttactcgg gaaatttgct tttgccaaac      240

ccgctttttc ggggatcccg cgcccccctc ctcacttgcg ctgctctcgg agccccagcc      300

ggctccgccc gcttcggcgg tttggatatt tattgacctc gtcctccgac tcgctgacag      360

gctacaggac ccccaacaac cccaatccac gttttggatg cactgagacc ccgacattcc      420

tcggtattta ttgtctgtcc ccacctagga cccccacccc cgaccctcgc gaataaaagg      480

ccctccatct gcccaaagct ctggactcca cagtgtccgc ggtttgcgtt gtgggccgga      540

ggctccgcag cgggccaatc cggaggcgtg tggaggcggc cgaaggtctg ggaggagcta      600

gcgggatgcg aagcggccga atcagggttg ggggaggaaa agccacgggg cggggctttg      660

gcgtccggcc aataggaggg cgagcgggcc acccggaggc accgcccccg cccagctgtg      720

gcccagctgt gccaccgagc gtcgagaaga gggggctggg ctggcagcgc gcgcggccat      780

cctccttcca ctgcgcctgc gcacgccacg cgcatccgct cctgggacgc aagctcgaga      840

aaagttgctg caaactttct agcccgttcc ccgcccctcc tcccggccag acccgccccc      900

cctgcggagc cgggaattcc gaggggcgga gcgcaggccg agatggggaa tgtgggggcc      960

tgcagaggac cctggagacg gaggcgtgca gaagctcagt ctcggggcgg aggcttcgcg     1020

cccttagtc                                                             1029


<210>  59
<211>  10474
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 expression plasmid

<400>  59
gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta       60

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag      120

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc      180

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga      240

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      300

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      360

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      420

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca      480

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat      540

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg      600

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg      660

agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg      720

actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgatatc      780

ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa      840

ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc      900

ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga      960

tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct     1020

aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa     1080

aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag     1140

tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg     1200

cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag     1260

ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact     1320

tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc     1380

ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg     1440

gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac     1500

tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac     1560

gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg     1620

gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag     1680

atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac     1740

ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc     1800

ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa     1860

ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta     1920

aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc     1980

caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc     2040

tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat     2100

gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag     2160

accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc     2220

atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac     2280

tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca     2340

gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc     2400

ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag     2460

attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg     2520

ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag     2580

aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg     2640

attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc     2700

aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga     2760

gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg     2820

aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca     2880

caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca     2940

gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg     3000

ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag     3060

aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg     3120

gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc     3180

tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat     3240

cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa agatgattct     3300

attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga taacgtcccc     3360

tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg     3420

atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg     3480

gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg     3540

gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga     3600

gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag     3660

ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca     3720

gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga     3780

gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag     3840

gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca     3900

ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa     3960

atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag     4020

gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc     4080

ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac     4140

ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa     4200

gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga     4260

tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa     4320

aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa     4380

cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa     4440

tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat     4500

aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag     4560

caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt     4620

tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac     4680

ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata     4740

gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca     4800

attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct     4860

gaccccaaga agaagaggaa ggtgtgaaag ggttcgatcc ctaccggtta gtaatgagtt     4920

taaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat     4980

gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg     5040

gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat     5100

acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc     5160

tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta     5220

gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc     5280

gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt     5340

cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag     5400

ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt     5460

cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt     5520

tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt     5580

cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt     5640

aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc     5700

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag     5760

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta     5820

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc     5880

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc     5940

ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggccgcca     6000

tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag     6060

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc     6120

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga     6180

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat     6240

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc     6300

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct     6360

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca     6420

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat     6480

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg     6540

cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag     6600

cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg     6660

cccgggatcc accggtcgcc accatgagcg agctgattaa ggagaacatg cacatgaagc     6720

tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca     6780

agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct     6840

tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca     6900

cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag     6960

tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg     7020

gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga     7080

tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg     7140

gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa     7200

acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct     7260

actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc     7320

agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta     7380

attaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact     7440

tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt     7500

tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa     7560

tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa     7620

tgtatcttaa ggcgcggacc gctatcagga catagcgttg gctacccgtg atattgctga     7680

agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga     7740

ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg     7800

ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg     7860

ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc     7920

tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt     7980

ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac     8040

tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt     8100

cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt     8160

atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg     8220

cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg     8280

gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc     8340

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc     8400

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata     8460

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg     8520

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     8580

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     8640

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     8700

tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt     8760

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     8820

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     8880

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     8940

tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc     9000

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     9060

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     9120

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     9180

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     9240

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     9300

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     9360

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     9420

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     9480

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     9540

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     9600

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     9660

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     9720

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     9780

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     9840

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     9900

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     9960

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga    10020

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg    10080

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat    10140

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc    10200

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca    10260

catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg    10320

gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc    10380

ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg    10440

cttgaccgac aattgcatga agaatctgct tagg                                10474


<210>  60
<211>  6399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Donor molecule

<400>  60
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt      180

gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt      240

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg      300

acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca      360

aggcctaggc gcgcctccct ggctcctggt ctccccgtcc agatataggg ctcaccctac      420

gtctttgcga ctttagaggg cagaagccct ttattcagcc ccagatctcc ctccgttcag      480

gcctcaccag attccctccg ggatctccct agataacctc cccaacctcg attcccctcg      540

ctgtctctcg ccccaccgct gagggctggg ctgggctccg atcgggtcac ctgtcccttc      600

tctctccagc tagatggccc cccggccgtg gctgtgggcc agtgcccgct ggtggggcca      660

ggccccatgc accgccgcca cctgctgctc cctgccaggg tacgtccggc tgcccacgcc      720

cccctccgcc gtcgcgcccc gcgctccacc cgccccttgc cacccgctta gctgcgcatt      780

tgcggggctg ggcccacggc aggagggcgg atcttcgggc agccaatcaa cacaggccgc      840

taggaagcag ccaatgacga gttcggacgg gattcgaggc gtgcgagtgg actaacaaca      900

gctgtaggct gttggggcgg gggcggggcg cagggaagag tgcgggccca cctatgggcg      960

taggcggggc gagtcccagg agccaatcag aggcccatgc cgggtgttga cctcgccctc     1020

tccccgcagg tccctaggcc tggcctatcg gaggcgcttt ccctgctcct gttcgccgtt     1080

gttctgtctc gtgccgccgc cctgggctgc attgggttgg tggcccacgc cggccaactc     1140

accgcagtct ggcgccgccc aggagccgcc cgcgctccct gaaccctaga actgtcttcg     1200

actccggggc cccgttggaa gactgagtgc ccggggcacg gcacagaagc cgcgcccacc     1260

gcctgccagt tcacaaccgc tccgagcgtg ggtctccgcc cagctccagt cctgtgatcc     1320

gggcccgccc cctagcggcc ggggagggag gggccgggtc cgcggccggc gaacggggct     1380

cgaagggtcc ttgtagccgg agctcccatg gcccaacttg tttattgcag cttataatgg     1440

ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc     1500

tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct cctgcagata     1560

acttcgtata gcatacatta tacgaagtta tattaagggt tccggatctc gaccagcttc     1620

tgatggaatt agaacttggc aaaacaatac tgagaatgaa gtgtatgtgg aacagaggct     1680

gctgatctcg ttcttcaggc tatgaaactg acacatttgg aaaccacagt acttagaacc     1740

acaaagtggg aatcaagaga aaaacaatga tcccacgaga gatctataga tctatagatc     1800

atgagtggga ggaatgagct ggcccttaat ttggttttgc ttgtttaaat tatgatatcc     1860

aactatgaaa cattatcata aagcaatagt aaagagcctt cagtaaagag caggcattta     1920

tctaatccca ccccaccccc acccccgtag ctccaatcct tccattcaaa atgtaggtac     1980

tctgttctca cccttcttaa caaagtatga caggaaaaac ttccatttta gtggacatct     2040

ttattgttta atagatcatc aatttctgca tcccggggat ctgatatcat cgatgcatgg     2100

ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc     2160

ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg     2220

aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc     2280

accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg     2340

ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg     2400

tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg     2460

gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc     2520

cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg     2580

cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg     2640

gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg     2700

acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagcttcagc     2760

tgctcgagat ctagatggat gcaggtcgaa aggcccggag atgaggaaga ggagaacagc     2820

gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg     2880

cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccaccctt cccagctgct     2940

gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct     3000

tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt     3060

gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag     3120

tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgctac cggtggatgt     3180

ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgccagcgg ggctgctaaa     3240

gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa tttcgaggtc     3300

gagatcctaa gcttggctgg acgtaaactc ctcttcagac ctaataactt cgtatagcat     3360

acattatacg aagttatgtc gacctgctgc tggggggatc acagaccatt tctttctttc     3420

ggccaggctg aggccctgac gtggatgggc aaactgcagg cctgggaagg cagcaagccg     3480

ggccgtccgt gttccatcct ccacgcaccc ccacctatcg ttggttcgca aagtgcaaag     3540

ctttcttgtg catgacgccc tgctctgggg agcgtctggc gcgatctctg cctgcttact     3600

cgggaaattt gcttttgcca aacccgcttt ttcggggatc ccgcgccccc ctcctcactt     3660

gcgctgctct cggagcccca gccggctccg cccgcttcgg cggtttggat atttattgac     3720

ctcgtcctcc gactcgctga caggctacag gacccccaac aaccccaatc cacgttttgg     3780

atgcactgag accccgacat tcctcggtat ttattgtctg tccccaccta ggacccccac     3840

ccccgaccct cgcgaataaa aggccctcca tctgcccaaa gctctggact ccacagtgtc     3900

cgcggtttgc gttgtgggcc ggaggctccg cagcgggcca atccggaggc gtgtggaggc     3960

ggccgaaggt ctgggaggag ctagcgggat gcgaagcggc cgaatcaggg ttgggggagg     4020

aaaagccacg gggcggggct ttggcgtccg gccaatagga gggcgagcgg gccacccgga     4080

ggcaccgccc ccgcccagct gtggcccagc tgtgccaccg agcgtcgaga agagggggct     4140

gggctggcag cgcgcgcggc catcctcctt ccactgcgcc tgcgcacgcc acgcgcatcc     4200

gctcctggga cgcaagctcg agaaaagttg ctgcaaactt tctagcccgt tccccgcccc     4260

tcctcccggc cagacccgcc ccccctgcgg agccgggaat tccgaggggc ggagcgcagg     4320

ccgagatggg gaatgtgggg gcctgcagag gaccctggag acggaggcgt gcagaagctc     4380

agtctcgggg cggaggcttc gcgcccttag tcggtacctc ttaattaact ggcctcatgg     4440

gccttccgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat     4500

ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg     4560

cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca     4620

ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc     4680

atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc     4740

aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg     4800

gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta     4860

ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg     4920

ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac     4980

acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag     5040

gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat     5100

ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat     5160

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     5220

gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt     5280

ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct     5340

agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt     5400

ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc     5460

gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac     5520

catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat     5580

cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg     5640

cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata     5700

gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta     5760

tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt     5820

gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag     5880

tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa     5940

gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc     6000

gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt     6060

taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc     6120

tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta     6180

ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa     6240

taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca     6300

tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac     6360

aaataggggt tccgcgcaca tttccccgaa aagtgccac                            6399


<210>  61
<211>  5250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Guide RNA 14189 expression plasmid

<400>  61
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag      360

ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc      420

cttattttaa cttgctattt ctagctctaa aacggctaca aggacccttc gacggtgttt      480

cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc      540

atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac      600

tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt      660

atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc      720

tcttcctgcc cgaccttggt accggatcca gtcgactgaa ttggttcctt taaagcctgc      780

ttttttgtac aaagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg      840

catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt      900

tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc      960

cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt     1020

tgcgcagcct atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc     1080

gtctgtttgt ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc     1140

ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc     1200

atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg     1260

ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta     1320

acctgatgtt ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct     1380

agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca     1440

gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca     1500

gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat     1560

tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt     1620

tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag     1680

gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg     1740

agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt     1800

tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc     1860

tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt     1920

gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag     1980

tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg     2040

ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag     2100

cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg     2160

atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga     2220

gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca     2280

tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc     2340

gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg     2400

ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct     2460

atcgccttct tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt     2520

ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat     2580

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     2640

agacaataac cctgataaat gcttcaataa tagcacgtga ggagggccac catggccgcc     2700

atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga     2760

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg     2820

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg     2880

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca     2940

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc     3000

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc     3060

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc     3120

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa     3180

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag     3240

gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta     3300

gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg     3360

gcccgggatc caccggtcgc caccatgccc gccatgaaga tcgagtgccg catcaccggc     3420

accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc cgagcagggc     3480

cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg     3540

agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac     3600

cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag     3660

gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc     3720

gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc     3780

atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc     3840

agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc     3900

cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc     3960

gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag     4020

cacgccttca agacccccat cgccttcgcc agatctcgag ctcgatgagc ggccgcgact     4080

ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc     4140

acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat     4200

tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt     4260

tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgcact     4320

tcgtggccga ggagcaggac tgacacgtgc taaaacttca tttttaattt aaaaggatct     4380

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc     4440

actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc     4500

gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg     4560

atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa     4620

atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc     4680

ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt     4740

gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa     4800

cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc     4860

tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc     4920

cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct     4980

ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat     5040

gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc     5100

tgggcttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg     5160

ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc     5220

gcagcgagtc agtgagcgag gaagcggaag                                      5250


<210>  62
<211>  5250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA 14254 expression plasmid

<400>  62
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc       60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc      120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa      180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat      240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca      300

ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag      360

ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc      420

cttattttaa cttgctattt ctagctctaa aacgcagcag cagcagcagc agcggtgttt      480

cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc      540

atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac      600

tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt      660

atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc      720

tcttcctgcc cgaccttggt accggatcca gtcgactgaa ttggttcctt taaagcctgc      780

ttttttgtac aaagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg      840

catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt      900

tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc      960

cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt     1020

tgcgcagcct atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc     1080

gtctgtttgt ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc     1140

ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc     1200

atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg     1260

ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta     1320

acctgatgtt ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct     1380

agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca     1440

gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca     1500

gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat     1560

tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt     1620

tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag     1680

gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg     1740

agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt     1800

tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc     1860

tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt     1920

gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag     1980

tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg     2040

ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag     2100

cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg     2160

atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga     2220

gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca     2280

tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc     2340

gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg     2400

ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct     2460

atcgccttct tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt     2520

ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat     2580

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg     2640

agacaataac cctgataaat gcttcaataa tagcacgtga ggagggccac catggccgcc     2700

atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga     2760

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg     2820

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg     2880

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca     2940

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc     3000

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc     3060

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc     3120

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa     3180

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag     3240

gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta     3300

gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg     3360

gcccgggatc caccggtcgc caccatgccc gccatgaaga tcgagtgccg catcaccggc     3420

accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc cgagcagggc     3480

cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg     3540

agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac     3600

cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag     3660

gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc     3720

gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc     3780

atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc     3840

agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc     3900

cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc     3960

gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag     4020

cacgccttca agacccccat cgccttcgcc agatctcgag ctcgatgagc ggccgcgact     4080

ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc     4140

acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat     4200

tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt     4260

tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgcact     4320

tcgtggccga ggagcaggac tgacacgtgc taaaacttca tttttaattt aaaaggatct     4380

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc     4440

actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc     4500

gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg     4560

atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa     4620

atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc     4680

ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt     4740

gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa     4800

cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc     4860

tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc     4920

cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct     4980

ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat     5040

gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc     5100

tgggcttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg     5160

ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc     5220

gcagcgagtc agtgagcgag gaagcggaag                                      5250


<210>  63
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  63
ccctcctagg ccgccatgca ttag                                              24


<210>  64
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  64
cccgttcggt ccgcgcctta agatacattg                                        30


<210>  65
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  65
cctctgcctc tgagctatt                                                    19


<210>  66
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  66
gataccgtaa agcacgagga a                                                 21


<210>  67
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  67
cccgtctgtc gacctgctgc tggggg                                            26


<210>  68
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  68
cccgtctgtc gacctgctgc tggggg                                            26


<210>  69
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  69
ctaagcttgg ctggacgta                                                    19


<210>  70
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  70
cctatggaaa aacgccagc                                                    19


<210>  71
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  71
ccttggcgcg cctccctggc tcct                                              24


<210>  72
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  72
ccctgagctc cggctacaag gac                                               23


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  73
gatgtgctgc aaggcgatta                                                   20


<210>  74
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  74
ccacaactag aatgcagtga aa                                                22


<210>  75
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cr14189_Insert_F annealing oligo

<400>  75
tttcttggct ttatatatct tgtggaaagg acgaaacacc gtcgaagggt ccttgtagcc       60


<210>  76
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cr14189_Insert_R annealing oligo

<400>  76
gactagcctt attttaactt gctatttcta gctctaaaac ggctacaagg acccttcgac       60


<210>  77
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  77
ccctggccac catggccgcc atgcattag                                         29


<210>  78
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  78
ccctcacgaa gtgcgcctta agatacattg                                        30


<210>  79
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  79
ccctcacgaa gtgcgcctta agatacattg                                        30


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  80
ggtatctgcg ctctgctgaa                                                   20


<210>  81
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  81
gtgtacggtg ggaggtctat                                                   20


<210>  82
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  82
caggaaacag ctatgacc                                                     18


<210>  83
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cr 14254 _Insert_F annealing oligo

<400>  83
tttcttggct ttatatatct tgtggaaagg acgaaacacc gctgctgctg ctgctgctgc       60


<210>  84
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cr 14254 _Insert_R annealing oligo

<400>  84
gactagcctt attttaactt gctatttcta gctctaaaac gcagcagcag cagcagcagc       60


<210>  85
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  85
ccctggccac catggccgcc atgcattag                                         29


<210>  86
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  86
ccctcacgaa gtgcgcctta agatacattg                                        30


<210>  87
<211>  305
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fragment guide RNA cloning vector

<400>  87
aggctttaaa ggaaccaatt cagtcgactg gatccggtac caaggtcggg caggaagagg       60

gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa      120

ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt      180

aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc      240

ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa      300

acacc                                                                  305


<210>  88
<211>  178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fragment guide RNA cloning vector

<400>  88
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt tttaagcttg ggccgctcga ggtacctctc tacatatgac      120

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgt        178


<210>  89
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  89
gctcgaaggg tccttgtagc cgg                                               23


<210>  90
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence  + PAM

<400>  90
gccggcgaac ggggctcgaa ggg                                               23


<210>  91
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  91
gggtccgcgg ccggcgaacg ggg                                               23


<210>  92
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  92
gccaggctga ggccctgacg tgg                                               23


<210>  93
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  93
gctgaggccc tgacgtggat ggg                                               23


<210>  94
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  94
gcagtttgcc catccacgtc agg                                               23


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  95
ggcgaacggg gctcgaaggg                                                   20


<210>  96
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  96
gtccgcggcc ggcgaacggg g                                                 21


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  97
gaggccctga cgtggatggg                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  98
gtttgcccat ccacgtcagg                                                   20


<210>  99
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  99
gttaaggctg ggaggcggga ggg                                               23


<210>  100
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  100
ggtcctcctg tcacagggcc tgg                                               23


<210>  101
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  101
gggcctggac aggggctgcc agg                                               23


<210>  102
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence  + PAM

<400>  102
gcatctcacc tctatgggag g                                                 21


<210>  103
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target sequence + PAM

<400>  103
ggcatctcac ctctatggga ggg                                               23


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1884 target seqeunce 5' CTG repeat

<400>  104
gctcgaaggg tccttgtagc                                                   20


<210>  105
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1885 target 5' CTG repeat

<400>  105
gccggcgaac ggggctcgaa                                                   20


<210>  106
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1886 target 5' CTG repeat

<400>  106
gggtccgcgg ccggcgaacg                                                   20


<210>  107
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1887 target 3' CTG repeat

<400>  107
gccaggctga ggccctgacg                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1888 target 3' CTG repeat

<400>  108
gctgaggccc tgacgtggat                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1889 target 3' CTG repeat

<400>  109
gcagtttgcc catccacgtc                                                   20


<210>  110
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1890 target 5' CTG repeat

<400>  110
ggcgaacggg gctcgaa                                                      17


<210>  111
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1891 target 5' CTG repeat

<400>  111
gtccgcggcc ggcgaacg                                                     18


<210>  112
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1892 target 3' CTG repeat

<400>  112
gaggccctga cgtggat                                                      17


<210>  113
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1881 target 3' CTG repeat

<400>  113
gtttgcccat ccacgtc                                                      17


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1896 target SP1

<400>  114
gttaaggctg ggaggcggga                                                   20


<210>  115
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1899 target AP2

<400>  115
ggtcctcctg tcacagggcc                                                   20


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1902 target AP2

<400>  116
gggcctggac aggggctgcc                                                   20


<210>  117
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1905 target ATG

<400>  117
gcatctcacc tctatggg                                                     18


<210>  118
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFYF1908 target ATG

<400>  118
ggcatctcac ctctatggga                                                   20


<210>  119
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  119
atcagctacg tacggactgg atccggtacc aagg                                   34


<210>  120
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  120
gtcgcagcta actagtccca agcttaaaaa aagcaccga                              39


<210>  121
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  generic tale structure

<400>  121
tttattccct gacc                                                         14


<210>  122
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  generic tale structure

<400>  122

Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
1               5                   10                  15      


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            20                  25                  30          


His Gly 
        


<210>  123
<211>  144
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMPK fragment

<400>  123
gggagggagg ggccgggtcc gcggccggcg aacggggctc gaagggtcct tgtagccggg       60

aatgctgctg ctgctgctgg ggggatatca gaccatttct ttctttcggc caggctgagg      120

ccctgacgtg gatgggcaaa ctgc                                             144


<210>  124
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMPK gene 3' region ctg repeat

<400>  124
gccaggctga ggccctgacg tggatgggca aactgc                                 36


<210>  125
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DMPK gene 3' region ctg repeat

<400>  125
gggtccgcgg ccggcgaacg gggctcgaag ggtccttgta gc                          42


<210>  126
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  scaffold part of sgRNA

<400>  126
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu                                                   80


