                         SEQUENCE LISTING

<110>  Oncomed Pharmaceuticals, Inc.
       GURNEY, Austin L.
       XIE, Ming-Hong
       PORTER, James Gordon
 
<120>  IMMUNOTHERAPY WITH BINDING AGENTS

<130>  2293.122PC01

<140>  PCT/US2015/030472
<141>  2015-05-13

<150>  61/992,456
<151>  2014-05-13

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  320
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human VSTM4 with predicted signal sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(23)
<223>  Predicted signal sequence

<400>  1

Met Arg Leu Leu Ala Leu Ala Ala Ala Ala Leu Leu Ala Arg Ala Pro 
1               5                   10                  15      


Ala Pro Glu Val Cys Ala Ala Leu Asn Val Thr Val Ser Pro Gly Pro 
            20                  25                  30          


Val Val Asp Tyr Leu Glu Gly Glu Asn Ala Thr Leu Leu Cys His Val 
        35                  40                  45              


Ser Gln Lys Arg Arg Lys Asp Ser Leu Leu Ala Val Arg Trp Phe Phe 
    50                  55                  60                  


Ala His Ser Phe Asp Ser Gln Glu Ala Leu Met Val Lys Met Thr Lys 
65                  70                  75                  80  


Leu Arg Val Val Gln Tyr Tyr Gly Asn Phe Ser Arg Ser Ala Lys Arg 
                85                  90                  95      


Arg Arg Leu Arg Leu Leu Glu Glu Gln Arg Gly Ala Leu Tyr Arg Leu 
            100                 105                 110         


Ser Val Leu Thr Leu Gln Pro Ser Asp Gln Gly His Tyr Val Cys Arg 
        115                 120                 125             


Val Gln Glu Ile Ser Arg His Arg Asn Lys Trp Thr Ala Trp Ser Asn 
    130                 135                 140                 


Gly Ser Ser Ala Thr Glu Met Arg Val Ile Ser Leu Lys Ala Ser Glu 
145                 150                 155                 160 


Glu Ser Ser Phe Glu Lys Thr Lys Glu Thr Trp Ala Phe Phe Glu Asp 
                165                 170                 175     


Leu Tyr Val Tyr Ala Val Leu Val Cys Cys Val Gly Ile Leu Ser Ile 
            180                 185                 190         


Leu Leu Phe Met Leu Val Ile Val Trp Gln Ser Val Phe Asn Lys Arg 
        195                 200                 205             


Lys Ser Arg Val Arg His Tyr Leu Val Lys Cys Pro Gln Asn Ser Ser 
    210                 215                 220                 


Gly Glu Thr Val Thr Ser Val Thr Ser Leu Ala Pro Leu Gln Pro Lys 
225                 230                 235                 240 


Lys Gly Lys Arg Gln Lys Glu Lys Pro Asp Ile Pro Pro Ala Val Pro 
                245                 250                 255     


Ala Lys Ala Pro Ile Ala Pro Thr Phe His Lys Pro Lys Leu Leu Lys 
            260                 265                 270         


Pro Gln Arg Lys Val Thr Leu Pro Lys Ile Ala Glu Glu Asn Leu Thr 
        275                 280                 285             


Tyr Ala Glu Leu Glu Leu Ile Lys Pro His Arg Ala Ala Lys Gly Ala 
    290                 295                 300                 


Pro Thr Ser Thr Val Tyr Ala Gln Ile Leu Phe Glu Glu Asn Lys Leu 
305                 310                 315                 320 


<210>  2
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human VSTM4 ECD (AA1-AA180) with predicted signal sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(23)
<223>  Predicted signal sequence

<400>  2

Met Arg Leu Leu Ala Leu Ala Ala Ala Ala Leu Leu Ala Arg Ala Pro 
1               5                   10                  15      


Ala Pro Glu Val Cys Ala Ala Leu Asn Val Thr Val Ser Pro Gly Pro 
            20                  25                  30          


Val Val Asp Tyr Leu Glu Gly Glu Asn Ala Thr Leu Leu Cys His Val 
        35                  40                  45              


Ser Gln Lys Arg Arg Lys Asp Ser Leu Leu Ala Val Arg Trp Phe Phe 
    50                  55                  60                  


Ala His Ser Phe Asp Ser Gln Glu Ala Leu Met Val Lys Met Thr Lys 
65                  70                  75                  80  


Leu Arg Val Val Gln Tyr Tyr Gly Asn Phe Ser Arg Ser Ala Lys Arg 
                85                  90                  95      


Arg Arg Leu Arg Leu Leu Glu Glu Gln Arg Gly Ala Leu Tyr Arg Leu 
            100                 105                 110         


Ser Val Leu Thr Leu Gln Pro Ser Asp Gln Gly His Tyr Val Cys Arg 
        115                 120                 125             


Val Gln Glu Ile Ser Arg His Arg Asn Lys Trp Thr Ala Trp Ser Asn 
    130                 135                 140                 


Gly Ser Ser Ala Thr Glu Met Arg Val Ile Ser Leu Lys Ala Ser Glu 
145                 150                 155                 160 


Glu Ser Ser Phe Glu Lys Thr Lys Glu Thr Trp Ala Phe Phe Glu Asp 
                165                 170                 175     


Leu Tyr Val Tyr 
            180 


<210>  3
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human VSTM4 ECD (AA24-AA180) without predicted signal sequence

<400>  3

Leu Asn Val Thr Val Ser Pro Gly Pro Val Val Asp Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Asn Ala Thr Leu Leu Cys His Val Ser Gln Lys Arg Arg Lys Asp 
            20                  25                  30          


Ser Leu Leu Ala Val Arg Trp Phe Phe Ala His Ser Phe Asp Ser Gln 
        35                  40                  45              


Glu Ala Leu Met Val Lys Met Thr Lys Leu Arg Val Val Gln Tyr Tyr 
    50                  55                  60                  


Gly Asn Phe Ser Arg Ser Ala Lys Arg Arg Arg Leu Arg Leu Leu Glu 
65                  70                  75                  80  


Glu Gln Arg Gly Ala Leu Tyr Arg Leu Ser Val Leu Thr Leu Gln Pro 
                85                  90                  95      


Ser Asp Gln Gly His Tyr Val Cys Arg Val Gln Glu Ile Ser Arg His 
            100                 105                 110         


Arg Asn Lys Trp Thr Ala Trp Ser Asn Gly Ser Ser Ala Thr Glu Met 
        115                 120                 125             


Arg Val Ile Ser Leu Lys Ala Ser Glu Glu Ser Ser Phe Glu Lys Thr 
    130                 135                 140                 


Lys Glu Thr Trp Ala Phe Phe Glu Asp Leu Tyr Val Tyr 
145                 150                 155         


<210>  4
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human VSTM4 Ig-like domain (AA24-AA155)

<400>  4

Leu Asn Val Thr Val Ser Pro Gly Pro Val Val Asp Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Asn Ala Thr Leu Leu Cys His Val Ser Gln Lys Arg Arg Lys Asp 
            20                  25                  30          


Ser Leu Leu Ala Val Arg Trp Phe Phe Ala His Ser Phe Asp Ser Gln 
        35                  40                  45              


Glu Ala Leu Met Val Lys Met Thr Lys Leu Arg Val Val Gln Tyr Tyr 
    50                  55                  60                  


Gly Asn Phe Ser Arg Ser Ala Lys Arg Arg Arg Leu Arg Leu Leu Glu 
65                  70                  75                  80  


Glu Gln Arg Gly Ala Leu Tyr Arg Leu Ser Val Leu Thr Leu Gln Pro 
                85                  90                  95      


Ser Asp Gln Gly His Tyr Val Cys Arg Val Gln Glu Ile Ser Arg His 
            100                 105                 110         


Arg Asn Lys Trp Thr Ala Trp Ser Asn Gly Ser Ser Ala Thr Glu Met 
        115                 120                 125             


Arg Val Ile Ser 
    130         


<210>  5
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human VSTM4 IgV domain (AA24-AA130)

<400>  5

Leu Asn Val Thr Val Ser Pro Gly Pro Val Val Asp Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Asn Ala Thr Leu Leu Cys His Val Ser Gln Lys Arg Arg Lys Asp 
            20                  25                  30          


Ser Leu Leu Ala Val Arg Trp Phe Phe Ala His Ser Phe Asp Ser Gln 
        35                  40                  45              


Glu Ala Leu Met Val Lys Met Thr Lys Leu Arg Val Val Gln Tyr Tyr 
    50                  55                  60                  


Gly Asn Phe Ser Arg Ser Ala Lys Arg Arg Arg Leu Arg Leu Leu Glu 
65                  70                  75                  80  


Glu Gln Arg Gly Ala Leu Tyr Arg Leu Ser Val Leu Thr Leu Gln Pro 
                85                  90                  95      


Ser Asp Gln Gly His Tyr Val Cys Arg Val Gln 
            100                 105         


<210>  6
<211>  319
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<223>  Mouse VSTM4 with predicted signal sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(23)
<223>  Predicted signal sequence

<400>  6

Met Arg Leu Arg Leu Leu Ala Leu Ala Ala Ala Val Leu Leu Gly Pro 
1               5                   10                  15      


Ala Pro Glu Val Cys Gly Ala Leu Asn Val Thr Val Ser Pro Gly Pro 
            20                  25                  30          


Val Val Asp Tyr Leu Glu Gly Glu Asn Ala Thr Leu Leu Cys His Val 
        35                  40                  45              


Ser Gln Lys Arg Arg Lys Asp Ser Leu Leu Ala Val Arg Trp Phe Phe 
    50                  55                  60                  


Ala Pro Asp Gly Ser Gln Glu Ala Leu Met Val Lys Met Thr Lys Leu 
65                  70                  75                  80  


Arg Ile Ile Gln Tyr Tyr Gly Asn Phe Ser Arg Thr Ala Asn Gln Gln 
                85                  90                  95      


Arg Leu Arg Leu Leu Glu Glu Arg Arg Gly Val Leu Tyr Arg Leu Ser 
            100                 105                 110         


Val Leu Thr Leu Arg Pro Thr Asp Gln Gly Gln Tyr Val Cys Lys Val 
        115                 120                 125             


Gln Glu Ile Ser Lys His Arg Asn Lys Trp Thr Ala Trp Ser Asn Gly 
    130                 135                 140                 


Ser Ser Ala Thr Glu Met Arg Val Ile Ser Leu Lys Ala Gly Glu Asp 
145                 150                 155                 160 


Ser Ser Phe Glu Lys Lys Lys Val Thr Trp Ala Phe Phe Glu Asp Leu 
                165                 170                 175     


Tyr Val Tyr Ala Val Leu Val Cys Cys Val Gly Ile Leu Ser Val Leu 
            180                 185                 190         


Leu Phe Thr Leu Val Ile Ala Trp Gln Ser Val Phe His Lys Arg Lys 
        195                 200                 205             


Ser Arg Val Arg His Tyr Leu Val Lys Cys Pro Gln Asn Ser Ser Gly 
    210                 215                 220                 


Glu Thr Val Thr Ser Val Thr Ser Leu Ala Pro Leu Gln Pro Gln Lys 
225                 230                 235                 240 


Gly Lys Arg Gln Lys Lys Lys Val Asp Val Pro Pro Ala Val Pro Ala 
                245                 250                 255     


Lys Ala Pro Ile Ala Thr Thr Phe His Lys Pro Lys Leu Leu Lys Pro 
            260                 265                 270         


Gln Arg Lys Val Ala Leu Pro Lys Ile Thr Glu Glu Asn Leu Thr Tyr 
        275                 280                 285             


Ala Glu Leu Glu Leu Ile Lys Pro His Arg Ala Ala Lys Gly Val Pro 
    290                 295                 300                 


Thr Ser Thr Val Tyr Ala Gln Ile Leu Phe Glu Glu Asn Gln Leu 
305                 310                 315                 


<210>  7
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse VSTM4 ECD (AA1-AA179) with predicted signal sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(23)
<223>  Predicted signal sequence

<400>  7

Met Arg Leu Arg Leu Leu Ala Leu Ala Ala Ala Val Leu Leu Gly Pro 
1               5                   10                  15      


Ala Pro Glu Val Cys Gly Ala Leu Asn Val Thr Val Ser Pro Gly Pro 
            20                  25                  30          


Val Val Asp Tyr Leu Glu Gly Glu Asn Ala Thr Leu Leu Cys His Val 
        35                  40                  45              


Ser Gln Lys Arg Arg Lys Asp Ser Leu Leu Ala Val Arg Trp Phe Phe 
    50                  55                  60                  


Ala Pro Asp Gly Ser Gln Glu Ala Leu Met Val Lys Met Thr Lys Leu 
65                  70                  75                  80  


Arg Ile Ile Gln Tyr Tyr Gly Asn Phe Ser Arg Thr Ala Asn Gln Gln 
                85                  90                  95      


Arg Leu Arg Leu Leu Glu Glu Arg Arg Gly Val Leu Tyr Arg Leu Ser 
            100                 105                 110         


Val Leu Thr Leu Arg Pro Thr Asp Gln Gly Gln Tyr Val Cys Lys Val 
        115                 120                 125             


Gln Glu Ile Ser Lys His Arg Asn Lys Trp Thr Ala Trp Ser Asn Gly 
    130                 135                 140                 


Ser Ser Ala Thr Glu Met Arg Val Ile Ser Leu Lys Ala Gly Glu Asp 
145                 150                 155                 160 


Ser Ser Phe Glu Lys Lys Lys Val Thr Trp Ala Phe Phe Glu Asp Leu 
                165                 170                 175     


Tyr Val Tyr 
            


<210>  8
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse VSTM4 ECD (AA24-AA179) without predicted signal sequence

<400>  8

Leu Asn Val Thr Val Ser Pro Gly Pro Val Val Asp Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Asn Ala Thr Leu Leu Cys His Val Ser Gln Lys Arg Arg Lys Asp 
            20                  25                  30          


Ser Leu Leu Ala Val Arg Trp Phe Phe Ala Pro Asp Gly Ser Gln Glu 
        35                  40                  45              


Ala Leu Met Val Lys Met Thr Lys Leu Arg Ile Ile Gln Tyr Tyr Gly 
    50                  55                  60                  


Asn Phe Ser Arg Thr Ala Asn Gln Gln Arg Leu Arg Leu Leu Glu Glu 
65                  70                  75                  80  


Arg Arg Gly Val Leu Tyr Arg Leu Ser Val Leu Thr Leu Arg Pro Thr 
                85                  90                  95      


Asp Gln Gly Gln Tyr Val Cys Lys Val Gln Glu Ile Ser Lys His Arg 
            100                 105                 110         


Asn Lys Trp Thr Ala Trp Ser Asn Gly Ser Ser Ala Thr Glu Met Arg 
        115                 120                 125             


Val Ile Ser Leu Lys Ala Gly Glu Asp Ser Ser Phe Glu Lys Lys Lys 
    130                 135                 140                 


Val Thr Trp Ala Phe Phe Glu Asp Leu Tyr Val Tyr 
145                 150                 155     


<210>  9
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse VSTM4 Ig-like domain (AA24-AA154)

<400>  9

Leu Asn Val Thr Val Ser Pro Gly Pro Val Val Asp Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Asn Ala Thr Leu Leu Cys His Val Ser Gln Lys Arg Arg Lys Asp 
            20                  25                  30          


Ser Leu Leu Ala Val Arg Trp Phe Phe Ala Pro Asp Gly Ser Gln Glu 
        35                  40                  45              


Ala Leu Met Val Lys Met Thr Lys Leu Arg Ile Ile Gln Tyr Tyr Gly 
    50                  55                  60                  


Asn Phe Ser Arg Thr Ala Asn Gln Gln Arg Leu Arg Leu Leu Glu Glu 
65                  70                  75                  80  


Arg Arg Gly Val Leu Tyr Arg Leu Ser Val Leu Thr Leu Arg Pro Thr 
                85                  90                  95      


Asp Gln Gly Gln Tyr Val Cys Lys Val Gln Glu Ile Ser Lys His Arg 
            100                 105                 110         


Asn Lys Trp Thr Ala Trp Ser Asn Gly Ser Ser Ala Thr Glu Met Arg 
        115                 120                 125             


Val Ile Ser 
    130     


<210>  10
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse VSTM4 Ig-like V domain (AA24-AA129)

<400>  10

Leu Asn Val Thr Val Ser Pro Gly Pro Val Val Asp Tyr Leu Glu Gly 
1               5                   10                  15      


Glu Asn Ala Thr Leu Leu Cys His Val Ser Gln Lys Arg Arg Lys Asp 
            20                  25                  30          


Ser Leu Leu Ala Val Arg Trp Phe Phe Ala Pro Asp Gly Ser Gln Glu 
        35                  40                  45              


Ala Leu Met Val Lys Met Thr Lys Leu Arg Ile Ile Gln Tyr Tyr Gly 
    50                  55                  60                  


Asn Phe Ser Arg Thr Ala Asn Gln Gln Arg Leu Arg Leu Leu Glu Glu 
65                  70                  75                  80  


Arg Arg Gly Val Leu Tyr Arg Leu Ser Val Leu Thr Leu Arg Pro Thr 
                85                  90                  95      


Asp Gln Gly Gln Tyr Val Cys Lys Val Gln 
            100                 105     


<210>  11
<211>  282
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human B7-H4 with predicted signal sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(24)
<223>  Predicted signal sequence

<400>  11

Met Ala Ser Leu Gly Gln Ile Leu Phe Trp Ser Ile Ile Ser Ile Ile 
1               5                   10                  15      


Ile Ile Leu Ala Gly Ala Ile Ala Leu Ile Ile Gly Phe Gly Ile Ser 
            20                  25                  30          


Gly Arg His Ser Ile Thr Val Thr Thr Val Ala Ser Ala Gly Asn Ile 
        35                  40                  45              


Gly Glu Asp Gly Ile Leu Ser Cys Thr Phe Glu Pro Asp Ile Lys Leu 
    50                  55                  60                  


Ser Asp Ile Val Ile Gln Trp Leu Lys Glu Gly Val Leu Gly Leu Val 
65                  70                  75                  80  


His Glu Phe Lys Glu Gly Lys Asp Glu Leu Ser Glu Gln Asp Glu Met 
                85                  90                  95      


Phe Arg Gly Arg Thr Ala Val Phe Ala Asp Gln Val Ile Val Gly Asn 
            100                 105                 110         


Ala Ser Leu Arg Leu Lys Asn Val Gln Leu Thr Asp Ala Gly Thr Tyr 
        115                 120                 125             


Lys Cys Tyr Ile Ile Thr Ser Lys Gly Lys Gly Asn Ala Asn Leu Glu 
    130                 135                 140                 


Tyr Lys Thr Gly Ala Phe Ser Met Pro Glu Val Asn Val Asp Tyr Asn 
145                 150                 155                 160 


Ala Ser Ser Glu Thr Leu Arg Cys Glu Ala Pro Arg Trp Phe Pro Gln 
                165                 170                 175     


Pro Thr Val Val Trp Ala Ser Gln Val Asp Gln Gly Ala Asn Phe Ser 
            180                 185                 190         


Glu Val Ser Asn Thr Ser Phe Glu Leu Asn Ser Glu Asn Val Thr Met 
        195                 200                 205             


Lys Val Val Ser Val Leu Tyr Asn Val Thr Ile Asn Asn Thr Tyr Ser 
    210                 215                 220                 


Cys Met Ile Glu Asn Asp Ile Ala Lys Ala Thr Gly Asp Ile Lys Val 
225                 230                 235                 240 


Thr Glu Ser Glu Ile Lys Arg Arg Ser His Leu Gln Leu Leu Asn Ser 
                245                 250                 255     


Lys Ala Ser Leu Cys Val Ser Ser Phe Phe Ala Ile Ser Trp Ala Leu 
            260                 265                 270         


Leu Pro Leu Ser Pro Tyr Leu Met Leu Lys 
        275                 280         


<210>  12
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human B7-H4 ECD (AA1-AA259) with predicted signal sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(24)
<223>  Predicted signal sequence

<400>  12

Met Ala Ser Leu Gly Gln Ile Leu Phe Trp Ser Ile Ile Ser Ile Ile 
1               5                   10                  15      


Ile Ile Leu Ala Gly Ala Ile Ala Leu Ile Ile Gly Phe Gly Ile Ser 
            20                  25                  30          


Gly Arg His Ser Ile Thr Val Thr Thr Val Ala Ser Ala Gly Asn Ile 
        35                  40                  45              


Gly Glu Asp Gly Ile Leu Ser Cys Thr Phe Glu Pro Asp Ile Lys Leu 
    50                  55                  60                  


Ser Asp Ile Val Ile Gln Trp Leu Lys Glu Gly Val Leu Gly Leu Val 
65                  70                  75                  80  


His Glu Phe Lys Glu Gly Lys Asp Glu Leu Ser Glu Gln Asp Glu Met 
                85                  90                  95      


Phe Arg Gly Arg Thr Ala Val Phe Ala Asp Gln Val Ile Val Gly Asn 
            100                 105                 110         


Ala Ser Leu Arg Leu Lys Asn Val Gln Leu Thr Asp Ala Gly Thr Tyr 
        115                 120                 125             


Lys Cys Tyr Ile Ile Thr Ser Lys Gly Lys Gly Asn Ala Asn Leu Glu 
    130                 135                 140                 


Tyr Lys Thr Gly Ala Phe Ser Met Pro Glu Val Asn Val Asp Tyr Asn 
145                 150                 155                 160 


Ala Ser Ser Glu Thr Leu Arg Cys Glu Ala Pro Arg Trp Phe Pro Gln 
                165                 170                 175     


Pro Thr Val Val Trp Ala Ser Gln Val Asp Gln Gly Ala Asn Phe Ser 
            180                 185                 190         


Glu Val Ser Asn Thr Ser Phe Glu Leu Asn Ser Glu Asn Val Thr Met 
        195                 200                 205             


Lys Val Val Ser Val Leu Tyr Asn Val Thr Ile Asn Asn Thr Tyr Ser 
    210                 215                 220                 


Cys Met Ile Glu Asn Asp Ile Ala Lys Ala Thr Gly Asp Ile Lys Val 
225                 230                 235                 240 


Thr Glu Ser Glu Ile Lys Arg Arg Ser His Leu Gln Leu Leu Asn Ser 
                245                 250                 255     


Lys Ala Ser 
            


<210>  13
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human B7-H4 ECD (AA25-AA259) without predicted signal sequence

<400>  13

Leu Ile Ile Gly Phe Gly Ile Ser Gly Arg His Ser Ile Thr Val Thr 
1               5                   10                  15      


Thr Val Ala Ser Ala Gly Asn Ile Gly Glu Asp Gly Ile Leu Ser Cys 
            20                  25                  30          


Thr Phe Glu Pro Asp Ile Lys Leu Ser Asp Ile Val Ile Gln Trp Leu 
        35                  40                  45              


Lys Glu Gly Val Leu Gly Leu Val His Glu Phe Lys Glu Gly Lys Asp 
    50                  55                  60                  


Glu Leu Ser Glu Gln Asp Glu Met Phe Arg Gly Arg Thr Ala Val Phe 
65                  70                  75                  80  


Ala Asp Gln Val Ile Val Gly Asn Ala Ser Leu Arg Leu Lys Asn Val 
                85                  90                  95      


Gln Leu Thr Asp Ala Gly Thr Tyr Lys Cys Tyr Ile Ile Thr Ser Lys 
            100                 105                 110         


Gly Lys Gly Asn Ala Asn Leu Glu Tyr Lys Thr Gly Ala Phe Ser Met 
        115                 120                 125             


Pro Glu Val Asn Val Asp Tyr Asn Ala Ser Ser Glu Thr Leu Arg Cys 
    130                 135                 140                 


Glu Ala Pro Arg Trp Phe Pro Gln Pro Thr Val Val Trp Ala Ser Gln 
145                 150                 155                 160 


Val Asp Gln Gly Ala Asn Phe Ser Glu Val Ser Asn Thr Ser Phe Glu 
                165                 170                 175     


Leu Asn Ser Glu Asn Val Thr Met Lys Val Val Ser Val Leu Tyr Asn 
            180                 185                 190         


Val Thr Ile Asn Asn Thr Tyr Ser Cys Met Ile Glu Asn Asp Ile Ala 
        195                 200                 205             


Lys Ala Thr Gly Asp Ile Lys Val Thr Glu Ser Glu Ile Lys Arg Arg 
    210                 215                 220                 


Ser His Leu Gln Leu Leu Asn Ser Lys Ala Ser 
225                 230                 235 


<210>  14
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human B7-H4 Ig-like V domain 1 (AA35-AA146)

<400>  14

His Ser Ile Thr Val Thr Thr Val Ala Ser Ala Gly Asn Ile Gly Glu 
1               5                   10                  15      


Asp Gly Ile Leu Ser Cys Thr Phe Glu Pro Asp Ile Lys Leu Ser Asp 
            20                  25                  30          


Ile Val Ile Gln Trp Leu Lys Glu Gly Val Leu Gly Leu Val His Glu 
        35                  40                  45              


Phe Lys Glu Gly Lys Asp Glu Leu Ser Glu Gln Asp Glu Met Phe Arg 
    50                  55                  60                  


Gly Arg Thr Ala Val Phe Ala Asp Gln Val Ile Val Gly Asn Ala Ser 
65                  70                  75                  80  


Leu Arg Leu Lys Asn Val Gln Leu Thr Asp Ala Gly Thr Tyr Lys Cys 
                85                  90                  95      


Tyr Ile Ile Thr Ser Lys Gly Lys Gly Asn Ala Asn Leu Glu Tyr Lys 
            100                 105                 110         


<210>  15
<211>  89
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human B7-H4 Ig-like V domain 2 (AA153-AA241)

<400>  15

Pro Glu Val Asn Val Asp Tyr Asn Ala Ser Ser Glu Thr Leu Arg Cys 
1               5                   10                  15      


Glu Ala Pro Arg Trp Phe Pro Gln Pro Thr Val Val Trp Ala Ser Gln 
            20                  25                  30          


Val Asp Gln Gly Ala Asn Phe Ser Glu Val Ser Asn Thr Ser Phe Glu 
        35                  40                  45              


Leu Asn Ser Glu Asn Val Thr Met Lys Val Val Ser Val Leu Tyr Asn 
    50                  55                  60                  


Val Thr Ile Asn Asn Thr Tyr Ser Cys Met Ile Glu Asn Asp Ile Ala 
65                  70                  75                  80  


Lys Ala Thr Gly Asp Ile Lys Val Thr 
                85                  


<210>  16
<211>  283
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<223>  Mouse B7-H4 with predicted signal sequence

<220>
<221>  MISC_FEATURE
<222>  (1)..(24)
<223>  Predicted signal sequence

<400>  16

Met Ala Ser Leu Gly Gln Ile Ile Phe Trp Ser Ile Ile Asn Ile Ile 
1               5                   10                  15      


Ile Ile Leu Ala Gly Ala Ile Ala Leu Ile Ile Gly Phe Gly Ile Ser 
            20                  25                  30          


Gly Lys His Phe Ile Thr Val Thr Thr Phe Thr Ser Ala Gly Asn Ile 
        35                  40                  45              


Gly Glu Asp Gly Thr Leu Ser Cys Thr Phe Glu Pro Asp Ile Lys Leu 
    50                  55                  60                  


Asn Gly Ile Val Ile Gln Trp Leu Lys Glu Gly Ile Lys Gly Leu Val 
65                  70                  75                  80  


His Glu Phe Lys Glu Gly Lys Asp Asp Leu Ser Gln Gln His Glu Met 
                85                  90                  95      


Phe Arg Gly Arg Thr Ala Val Phe Ala Asp Gln Val Val Val Gly Asn 
            100                 105                 110         


Ala Ser Leu Arg Leu Lys Asn Val Gln Leu Thr Asp Ala Gly Thr Tyr 
        115                 120                 125             


Thr Cys Tyr Ile Arg Thr Ser Lys Gly Lys Gly Asn Ala Asn Leu Glu 
    130                 135                 140                 


Tyr Lys Thr Gly Ala Phe Ser Met Pro Glu Ile Asn Val Asp Tyr Asn 
145                 150                 155                 160 


Ala Ser Ser Glu Ser Leu Arg Cys Glu Ala Pro Arg Trp Phe Pro Gln 
                165                 170                 175     


Pro Thr Val Ala Trp Ala Ser Gln Val Asp Gln Gly Ala Asn Phe Ser 
            180                 185                 190         


Glu Val Ser Asn Thr Ser Phe Glu Leu Asn Ser Glu Asn Val Thr Met 
        195                 200                 205             


Lys Val Val Ser Val Leu Tyr Asn Val Thr Ile Asn Asn Thr Tyr Ser 
    210                 215                 220                 


Cys Met Ile Glu Asn Asp Ile Ala Lys Ala Thr Gly Asp Ile Lys Val 
225                 230                 235                 240 


Thr Asp Ser Glu Val Lys Arg Arg Ser Gln Leu Gln Leu Leu Asn Ser 
                245                 250                 255     


Gly Pro Ser Pro Cys Val Phe Ser Ser Ala Phe Val Ala Gly Trp Ala 
            260                 265                 270         


Leu Leu Ser Leu Ser Cys Cys Leu Met Leu Arg 
        275                 280             


<210>  17
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse B7-H4 ECD (AA1-AA257) with predicted signal sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(24)
<223>  Predicted signal sequence

<400>  17

Met Ala Ser Leu Gly Gln Ile Ile Phe Trp Ser Ile Ile Asn Ile Ile 
1               5                   10                  15      


Ile Ile Leu Ala Gly Ala Ile Ala Leu Ile Ile Gly Phe Gly Ile Ser 
            20                  25                  30          


Gly Lys His Phe Ile Thr Val Thr Thr Phe Thr Ser Ala Gly Asn Ile 
        35                  40                  45              


Gly Glu Asp Gly Thr Leu Ser Cys Thr Phe Glu Pro Asp Ile Lys Leu 
    50                  55                  60                  


Asn Gly Ile Val Ile Gln Trp Leu Lys Glu Gly Ile Lys Gly Leu Val 
65                  70                  75                  80  


His Glu Phe Lys Glu Gly Lys Asp Asp Leu Ser Gln Gln His Glu Met 
                85                  90                  95      


Phe Arg Gly Arg Thr Ala Val Phe Ala Asp Gln Val Val Val Gly Asn 
            100                 105                 110         


Ala Ser Leu Arg Leu Lys Asn Val Gln Leu Thr Asp Ala Gly Thr Tyr 
        115                 120                 125             


Thr Cys Tyr Ile Arg Thr Ser Lys Gly Lys Gly Asn Ala Asn Leu Glu 
    130                 135                 140                 


Tyr Lys Thr Gly Ala Phe Ser Met Pro Glu Ile Asn Val Asp Tyr Asn 
145                 150                 155                 160 


Ala Ser Ser Glu Ser Leu Arg Cys Glu Ala Pro Arg Trp Phe Pro Gln 
                165                 170                 175     


Pro Thr Val Ala Trp Ala Ser Gln Val Asp Gln Gly Ala Asn Phe Ser 
            180                 185                 190         


Glu Val Ser Asn Thr Ser Phe Glu Leu Asn Ser Glu Asn Val Thr Met 
        195                 200                 205             


Lys Val Val Ser Val Leu Tyr Asn Val Thr Ile Asn Asn Thr Tyr Ser 
    210                 215                 220                 


Cys Met Ile Glu Asn Asp Ile Ala Lys Ala Thr Gly Asp Ile Lys Val 
225                 230                 235                 240 


Thr Asp Ser Glu Val Lys Arg Arg Ser Gln Leu Gln Leu Leu Asn Ser 
                245                 250                 255     


Gly 
    


<210>  18
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse B7-H4 ECD (AA25-AA257) without predicted signal sequence

<400>  18

Leu Ile Ile Gly Phe Gly Ile Ser Gly Lys His Phe Ile Thr Val Thr 
1               5                   10                  15      


Thr Phe Thr Ser Ala Gly Asn Ile Gly Glu Asp Gly Thr Leu Ser Cys 
            20                  25                  30          


Thr Phe Glu Pro Asp Ile Lys Leu Asn Gly Ile Val Ile Gln Trp Leu 
        35                  40                  45              


Lys Glu Gly Ile Lys Gly Leu Val His Glu Phe Lys Glu Gly Lys Asp 
    50                  55                  60                  


Asp Leu Ser Gln Gln His Glu Met Phe Arg Gly Arg Thr Ala Val Phe 
65                  70                  75                  80  


Ala Asp Gln Val Val Val Gly Asn Ala Ser Leu Arg Leu Lys Asn Val 
                85                  90                  95      


Gln Leu Thr Asp Ala Gly Thr Tyr Thr Cys Tyr Ile Arg Thr Ser Lys 
            100                 105                 110         


Gly Lys Gly Asn Ala Asn Leu Glu Tyr Lys Thr Gly Ala Phe Ser Met 
        115                 120                 125             


Pro Glu Ile Asn Val Asp Tyr Asn Ala Ser Ser Glu Ser Leu Arg Cys 
    130                 135                 140                 


Glu Ala Pro Arg Trp Phe Pro Gln Pro Thr Val Ala Trp Ala Ser Gln 
145                 150                 155                 160 


Val Asp Gln Gly Ala Asn Phe Ser Glu Val Ser Asn Thr Ser Phe Glu 
                165                 170                 175     


Leu Asn Ser Glu Asn Val Thr Met Lys Val Val Ser Val Leu Tyr Asn 
            180                 185                 190         


Val Thr Ile Asn Asn Thr Tyr Ser Cys Met Ile Glu Asn Asp Ile Ala 
        195                 200                 205             


Lys Ala Thr Gly Asp Ile Lys Val Thr Asp Ser Glu Val Lys Arg Arg 
    210                 215                 220                 


Ser Gln Leu Gln Leu Leu Asn Ser Gly 
225                 230             


<210>  19
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse B7-H4 Ig-like V-type domain 1 (AA35-AA144)

<400>  19

Lys His Phe Ile Thr Val Thr Thr Phe Thr Ser Ala Gly Asn Ile Gly 
1               5                   10                  15      


Glu Asp Gly Thr Leu Ser Cys Thr Phe Glu Pro Asp Ile Lys Leu Asn 
            20                  25                  30          


Gly Ile Val Ile Gln Trp Leu Lys Glu Gly Ile Lys Gly Leu Val His 
        35                  40                  45              


Glu Phe Lys Glu Gly Lys Asp Asp Leu Ser Gln Gln His Glu Met Phe 
    50                  55                  60                  


Arg Gly Arg Thr Ala Val Phe Ala Asp Gln Val Val Val Gly Asn Ala 
65                  70                  75                  80  


Ser Leu Arg Leu Lys Asn Val Gln Leu Thr Asp Ala Gly Thr Tyr Thr 
                85                  90                  95      


Cys Tyr Ile Arg Thr Ser Lys Gly Lys Gly Asn Ala Asn Leu Glu 
            100                 105                 110     


<210>  20
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse B7-H4 Ig-like V-type domain 2 (AA153-AA241)

<400>  20

Trp Phe Pro Gln Pro Thr Val Ala Trp Ala Ser Gln Val Asp Gln Gly 
1               5                   10                  15      


Ala Asn Phe Ser Glu Val Ser Asn Thr Ser Phe Glu Leu Asn Ser Glu 
            20                  25                  30          


Asn Val Thr Met Lys Val Val Ser Val Leu Tyr Asn Val Thr Ile Asn 
        35                  40                  45              


Asn Thr Tyr Ser Cys Met Ile Glu Asn Asp Ile Ala Lys Ala Thr Gly 
    50                  55                  60                  


Asp Ile Lys Val Thr 
65                  


<210>  21
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc region

<400>  21

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  22
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc region

<400>  22

Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
1               5                   10                  15      


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            20                  25                  30          


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        35                  40                  45              


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    50                  55                  60                  


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
65                  70                  75                  80  


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                85                  90                  95      


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            100                 105                 110         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        115                 120                 125             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
    130                 135                 140                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
145                 150                 155                 160 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                165                 170                 175     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            180                 185                 190         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        195                 200                 205             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    210                 215                 220                 


Ser Leu Ser Pro Gly Lys 
225                 230 


<210>  23
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc region

<400>  23

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  24
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Fc region

<400>  24

Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser 
1               5                   10                  15      


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            20                  25                  30          


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
        35                  40                  45              


Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    50                  55                  60                  


Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val 
65                  70                  75                  80  


Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                85                  90                  95      


Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr 
            100                 105                 110         


Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        115                 120                 125             


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
    130                 135                 140                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
145                 150                 155                 160 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp 
                165                 170                 175     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
            180                 185                 190         


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        195                 200                 205             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220                 


<210>  25
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse IgG1 Fc region

<400>  25

Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe 
1               5                   10                  15      


Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro 
            20                  25                  30          


Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val 
        35                  40                  45              


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr 
    50                  55                  60                  


Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu 
65                  70                  75                  80  


Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 
                85                  90                  95      


Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            100                 105                 110         


Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro 
        115                 120                 125             


Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile 
    130                 135                 140                 


Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly 
145                 150                 155                 160 


Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp 
                165                 170                 175     


Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp 
            180                 185                 190         


Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His 
        195                 200                 205             


Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
    210                 215                 220         


<210>  26
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse IgG2a Fc region

<400>  26

Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met 
            20                  25                  30          


Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu 
        35                  40                  45              


Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val 
    50                  55                  60                  


His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu 
65                  70                  75                  80  


Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly 
                85                  90                  95      


Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val 
        115                 120                 125             


Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr 
    130                 135                 140                 


Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu 
145                 150                 155                 160 


Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val 
            180                 185                 190         


Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val 
        195                 200                 205             


His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  27
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Heavy chain constant region

<400>  27

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  28
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Heavy chain constant region

<400>  28

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  29
<211>  377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG3 Heavy chain constant region

<400>  29

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro 
            100                 105                 110         


Arg Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
        115                 120                 125             


Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys 
    130                 135                 140                 


Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
145                 150                 155                 160 


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
                165                 170                 175     


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            180                 185                 190         


Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Lys Trp Tyr 
        195                 200                 205             


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    210                 215                 220                 


Gln Tyr Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Leu His 
225                 230                 235                 240 


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                245                 250                 255     


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln 
            260                 265                 270         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
        275                 280                 285             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    290                 295                 300                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Ser Gly Gln Pro Glu Asn Asn 
305                 310                 315                 320 


Tyr Asn Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Ile 
            340                 345                 350         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Arg Phe Thr Gln 
        355                 360                 365             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    370                 375         


<210>  30
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 Heavy chain constant region

<400>  30

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG Tag

<400>  31

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  32

Glu Ser Gly Gly Gly Gly Val Thr 
1               5               


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  33

Leu Glu Ser Gly Gly Gly Gly Val Thr 
1               5                   


<210>  34
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  34

Gly Arg Ala Gln Val Thr 
1               5       


<210>  35
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  35

Trp Arg Ala Gln Val Thr 
1               5       


<210>  36
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  36

Ala Arg Gly Arg Ala Gln Val Thr 
1               5               


<210>  37
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)
<223>  Xaa can be either Leu or Ile

<220>
<221>  MISC_FEATURE
<222>  (5)..()
<223>  Xaa can be any naturally occurring amino acid and is repeated 6-8 times


<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (9)
<223>  Xaa can be either Leu or Ile

<400>  37

Tyr Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa 
1               5                   


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITIM


<220>
<221>  MISC_FEATURE
<222>  (1)
<223>  Xaa is Ser, Ile, Val, or Leu

<220>
<221>  misc_feature
<222>  (3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)
<223>  Xaa can be Ile, Val, or Leu

<400>  38

Xaa Leu Xaa Tyr Xaa Xaa Xaa 
1               5           


<210>  39
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITSM

<220>
<221>  misc_feature
<222>  (2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)
<223>  Xaa is Val or Ile

<400>  39

Thr Xaa Tyr Xaa Xaa Xaa 
1               5       


