                         SEQUENCE LISTING

<110>  INSERM
 
<120>  ANTI- EGFR CONFORMATIONAL SINGLE DOMAIN ANTIBODIES AND USES 
       THEREOF

<130>  BIO 14074 BATY / MC

<160>  15    

<170>  PatentIn version 3.3

<210>  1
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  D10 CDR1

<400>  1

Ile Ser Arg Thr Ile Phe Ser Leu Tyr Ala 
1               5                   10  


<210>  2
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  D10 CDR2

<400>  2

Ile Tyr Arg Ser Gly Asp Thr 
1               5           


<210>  3
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  D10 CDR3

<400>  3

Asn Ser Pro Ala Gln Asp Trp Pro 
1               5               


<210>  4
<211>  116
<212>  PRT
<213>  Artificial

<220>
<223>  D10

<400>  4

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Ile Ser Arg Thr Ile Phe Ser 
            20                  25                  30          


Leu Tyr Ala Met Glu Trp Tyr Arg Gln Pro Pro Gly Lys Gln Arg Asp 
        35                  40                  45              


Leu Val Ala Arg Ile Tyr Arg Ser Gly Asp Thr Asn Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Gly Val Tyr Tyr 
                85                  90                  95      


Cys Asn Ser Pro Ala Gln Asp Trp Pro Trp Gly Gln Gly Thr Gln Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  5
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  G10 CDR1

<400>  5

Gly Arg Thr Phe Ser Asn Tyr Ala 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  G10 CDR2

<400>  6

Ile Ile Trp Ser Gly Ser Arg Thr 
1               5               


<210>  7
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  G10 CDR3

<400>  7

Ala Ala Ser Met Gly Asp Tyr Asp Val Ser Leu Ala Ser Pro Arg Ser 
1               5                   10                  15      


<210>  8
<211>  123
<212>  PRT
<213>  Artificial

<220>
<223>  G10

<400>  8

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Asn Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Ile Trp Ser Gly Ser Arg Thr Tyr Tyr Ala Asp Pro Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Asn Val Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ser Met Gly Asp Tyr Asp Val Ser Leu Ala Ser Pro Arg Ser 
            100                 105                 110         


Trp Gly Lys Gly Thr Gln Val Thr Val Ser Ser 
        115                 120             


<210>  9
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  E10 CDR1

<400>  9

Gly Arg Thr Leu Ser Ser Tyr Asp 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  E10 CDR2

<400>  10

Ile Asn Trp Gly Asp Leu Ser Thr 
1               5               


<210>  11
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  E10 CDR3

<400>  11

Ala Ala Arg Leu Arg Tyr Thr Val Ser Asp Pro Ile Phe Ser Arg Pro 
1               5                   10                  15      


Asp Arg Tyr Asn Tyr 
            20      


<210>  12
<211>  128
<212>  PRT
<213>  Artificial

<220>
<223>  E10

<400>  12

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu Ser Ser Tyr 
            20                  25                  30          


Asp Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Thr Ala Ile Asn Trp Gly Asp Leu Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Arg Leu Arg Tyr Thr Val Ser Asp Pro Ile Phe Ser Arg Pro 
            100                 105                 110         


Asp Arg Tyr Asn Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


<210>  13
<211>  284
<212>  PRT
<213>  Artificial

<220>
<223>  D2E

<400>  13

Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Ile Ser Arg Thr Ile Phe Ser 
            20                  25                  30          


Leu Tyr Ala Met Glu Trp Tyr Arg Gln Pro Pro Gly Lys Gln Arg Asp 
        35                  40                  45              


Leu Val Ala Arg Ile Tyr Arg Ser Gly Asp Thr Asn Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Gly Val Tyr Tyr 
                85                  90                  95      


Cys Asn Ser Pro Ala Gln Asp Trp Pro Trp Gly Gln Gly Thr Gln Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Met Ala Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Ala Gln 
    130                 135                 140                 


Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu 
145                 150                 155                 160 


Ser Ser Tyr Asp Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg 
                165                 170                 175     


Glu Phe Val Thr Ala Ile Asn Trp Gly Asp Leu Ser Thr Tyr Tyr Ala 
            180                 185                 190         


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
        195                 200                 205             


Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Ala Ala Arg Leu Arg Tyr Thr Val Ser Asp Pro Ile Phe 
225                 230                 235                 240 


Ser Arg Pro Asp Arg Tyr Asn Tyr Trp Gly Gln Gly Thr Gln Val Thr 
                245                 250                 255     


Val Ser Ser Ala Ala Ala Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
            260                 265                 270         


Asn Gly Ala Ala His His His His His His Gly Ser 
        275                 280                 


<210>  14
<211>  294
<212>  PRT
<213>  Artificial

<220>
<223>  D4E

<400>  14

Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Ile Ser Arg Thr Ile Phe Ser 
            20                  25                  30          


Leu Tyr Ala Met Glu Trp Tyr Arg Gln Pro Pro Gly Lys Gln Arg Asp 
        35                  40                  45              


Leu Val Ala Arg Ile Tyr Arg Ser Gly Asp Thr Asn Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Gly Val Tyr Tyr 
                85                  90                  95      


Cys Asn Ser Pro Ala Gln Asp Trp Pro Trp Gly Gln Gly Thr Gln Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ala Gln Val Gln 
    130                 135                 140                 


Leu Gln Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu Ser Ser Tyr Asp Met Gly 
                165                 170                 175     


Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Thr Ala Ile 
            180                 185                 190         


Asn Trp Gly Asp Leu Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg 
        195                 200                 205             


Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met 
    210                 215                 220                 


Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Ala Arg 
225                 230                 235                 240 


Leu Arg Tyr Thr Val Ser Asp Pro Ile Phe Ser Arg Pro Asp Arg Tyr 
                245                 250                 255     


Asn Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala 
            260                 265                 270         


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Gly Ala Ala His His 
        275                 280                 285             


His His His His Gly Ser 
    290                 


<210>  15
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  D6E

<400>  15

Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Ile Ser Arg Thr Ile Phe Ser 
            20                  25                  30          


Leu Tyr Ala Met Glu Trp Tyr Arg Gln Pro Pro Gly Lys Gln Arg Asp 
        35                  40                  45              


Leu Val Ala Arg Ile Tyr Arg Ser Gly Asp Thr Asn Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Gly Val Tyr Tyr 
                85                  90                  95      


Cys Asn Ser Pro Ala Gln Asp Trp Pro Trp Gly Gln Gly Thr Gln Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Met Ala Gln Val Gln Leu Gln Glu Ser Gly Gly 
145                 150                 155                 160 


Gly Leu Ala Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                165                 170                 175     


Gly Arg Thr Leu Ser Ser Tyr Asp Met Gly Trp Phe Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Glu Arg Glu Phe Val Thr Ala Ile Asn Trp Gly Asp Leu Ser 
        195                 200                 205             


Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Ala Ala Arg Leu Arg Tyr Thr Val Ser 
                245                 250                 255     


Asp Pro Ile Phe Ser Arg Pro Asp Arg Tyr Asn Tyr Trp Gly Gln Gly 
            260                 265                 270         


Thr Gln Val Thr Val Ser Ser Ala Ala Ala Glu Gln Lys Leu Ile Ser 
        275                 280                 285             


Glu Glu Asp Leu Asn Gly Ala Ala His His His His His His Gly Ser 
    290                 295                 300                 


