                         SEQUENCE LISTING

<110>  Imperial Innovations Limited
       Imperial College of Science, Technology & Medicine
       The Chancellor, Masters and Scholars of The University of 
       Oxford
       The University Court of The University of Edinburgh
       DNAVEC Corporation
 
<120>  Lentiviral Vectors

<130>  P42911WO

<150>  GB1409089.8
<151>  2014-05-21

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  10535
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM326 plasmid

<400>  1
ggtacctcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat       60

tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc      120

atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat      180

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      240

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      300

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      360

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt      420

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc      480

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      540

gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      600

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      660

caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc      720

tatataagca gagctcgctg gcttgtaact cagtctctta ctaggagacc agcttgagcc      780

tgggtgttcg ctggttagcc taacctggtt ggccaccagg ggtaaggact ccttggctta      840

gaaagctaat aaacttgcct gcattagagc ttatctgagt caagtgtcct cattgacgcc      900

tcactctctt gaacgggaat cttccttact gggttctctc tctgacccag gcgagagaaa      960

ctccagcagt ggcgcccgaa cagggacttg agtgagagtg taggcacgta cagctgagaa     1020

ggcgtcggac gcgaaggaag cgcggggtgc gacgcgacca agaaggagac ttggtgagta     1080

ggcttctcga gtgccgggaa aaagctcgag cctagttaga ggactaggag aggccgtagc     1140

cgtaactact cttgggcaag tagggcaggc ggtgggtacg caatgggggc ggctacctca     1200

gcactaaata ggagacaatt agaccaattt gagaaaatac gacttcgccc gaacggaaag     1260

aaaaagtacc aaattaaaca tttaatatgg gcaggcaagg agatggagcg cttcggcctc     1320

catgagaggt tgttggagac agaggagggg tgtaaaagaa tcatagaagt cctctacccc     1380

ctagaaccaa caggatcgga gggcttaaaa agtctgttca atcttgtgtg cgtgctatat     1440

tgcttgcaca aggaacagaa agtgaaagac acagaggaag cagtagcaac agtaagacaa     1500

cactgccatc tagtggaaaa agaaaaaagt gcaacagaga catctagtgg acaaaagaaa     1560

aatgacaagg gaatagcagc gccacctggt ggcagtcaga attttccagc gcaacaacaa     1620

ggaaatgcct gggtacatgt acccttgtca ccgcgcacct taaatgcgtg ggtaaaagca     1680

gtagaggaga aaaaatttgg agcagaaata gtacccattt ttttgtttca agccctatcg     1740

aattcccgtt tgtgctaggg ttcttaggct tcttgggggc tgctggaact gcaatgggag     1800

cagcggcgac agccctgacg gtccagtctc agcatttgct tgctgggata ctgcagcagc     1860

agaagaatct gctggcggct gtggaggctc aacagcagat gttgaagctg accatttggg     1920

gtgttaaaaa cctcaatgcc cgcgtcacag cccttgagaa gtacctagag gatcaggcac     1980

gactaaactc ctgggggtgc gcatggaaac aagtatgtca taccacagtg gagtggccct     2040

ggacaaatcg gactccggat tggcaaaata tgacttggtt ggagtgggaa agacaaatag     2100

ctgatttgga aagcaacatt acgagacaat tagtgaaggc tagagaacaa gaggaaaaga     2160

atctagatgc ctatcagaag ttaactagtt ggtcagattt ctggtcttgg ttcgatttct     2220

caaaatggct taacatttta aaaatgggat ttttagtaat agtaggaata atagggttaa     2280

gattacttta cacagtatat ggatgtatag tgagggttag gcagggatat gttcctctat     2340

ctccacagat ccatatccgc ggcaatttta aaagaaaggg aggaataggg ggacagactt     2400

cagcagagag actaattaat ataataacaa cacaattaga aatacaacat ttacaaacca     2460

aaattcaaaa aattttaaat tttagagccg cggagatctg ttacataact tatggtaaat     2520

ggcctgcctg gctgactgcc caatgacccc tgcccaatga tgtcaataat gatgtatgtt     2580

cccatgtaat gccaataggg actttccatt gatgtcaatg ggtggagtat ttatggtaac     2640

tgcccacttg gcagtacatc aagtgtatca tatgccaagt atgcccccta ttgatgtcaa     2700

tgatggtaaa tggcctgcct ggcattatgc ccagtacatg accttatggg actttcctac     2760

ttggcagtac atctatgtat tagtcattgc tattaccatg ggaattcact agtggagaag     2820

agcatgcttg agggctgagt gcccctcagt gggcagagag cacatggccc acagtccctg     2880

agaagttggg gggaggggtg ggcaattgaa ctggtgccta gagaaggtgg ggcttgggta     2940

aactgggaaa gtgatgtggt gtactggctc cacctttttc cccagggtgg gggagaacca     3000

tatataagtg cagtagtctc tgtgaacatt caagcttctg ccttctccct cctgtgagtt     3060

tgctagccac catgcagaga agccctctgg agaaggcctc tgtggtgagc aagctgttct     3120

tcagctggac caggcccatc ctgaggaagg gctacaggca gagactggag ctgtctgaca     3180

tctaccagat cccctctgtg gactctgctg acaacctgtc tgagaagctg gagagggagt     3240

gggatagaga gctggccagc aagaagaacc ccaagctgat caatgccctg aggagatgct     3300

tcttctggag attcatgttc tatggcatct tcctgtacct gggggaagtg accaaggctg     3360

tgcagcctct gctgctgggc agaatcattg ccagctatga ccctgacaac aaggaggaga     3420

ggagcattgc catctacctg ggcattggcc tgtgcctgct gttcattgtg aggaccctgc     3480

tgctgcaccc tgccatcttt ggcctgcacc acattggcat gcagatgagg attgccatgt     3540

tcagcctgat ctacaagaaa accctgaagc tgtccagcag agtgctggac aagatcagca     3600

ttggccagct ggtgagcctg ctgagcaaca acctgaacaa gtttgatgag ggcctggccc     3660

tggcccactt tgtgtggatt gcccctctgc aggtggccct gctgatgggc ctgatttggg     3720

agctgctgca ggcctctgcc ttttgtggcc tgggcttcct gattgtgctg gccctgtttc     3780

aggctggcct gggcaggatg atgatgaagt acagggacca gagggcaggc aagatcagtg     3840

agaggctggt gatcacctct gagatgattg agaacatcca gtctgtgaag gcctactgtt     3900

gggaggaagc tatggagaag atgattgaaa acctgaggca gacagagctg aagctgacca     3960

ggaaggctgc ctatgtgaga tacttcaaca gctctgcctt cttcttctct ggcttctttg     4020

tggtgttcct gtctgtgctg ccctatgccc tgatcaaggg gatcatcctg agaaagattt     4080

tcaccaccat cagcttctgc attgtgctga ggatggctgt gaccagacag ttcccctggg     4140

ctgtgcagac ctggtatgac agcctggggg ccatcaacaa gatccaggac ttcctgcaga     4200

agcaggagta caagaccctg gagtacaacc tgaccaccac agaagtggtg atggagaatg     4260

tgacagcctt ctgggaggag ggctttgggg agctgtttga gaaggccaag cagaacaaca     4320

acaacagaaa gaccagcaat ggggatgact ccctgttctt ctccaacttc tccctgctgg     4380

gcacacctgt gctgaaggac atcaacttca agattgagag ggggcagctg ctggctgtgg     4440

ctggatctac aggggctggc aagaccagcc tgctgatgat gatcatgggg gagctggagc     4500

cttctgaggg caagatcaag cactctggca ggatcagctt ttgcagccag ttcagctgga     4560

tcatgcctgg caccatcaag gagaacatca tctttggagt gagctatgat gagtacagat     4620

acaggagtgt gatcaaggcc tgccagctgg aggaggacat cagcaagttt gctgagaagg     4680

acaacattgt gctgggggag ggaggcatta cactgtctgg gggccagaga gccagaatca     4740

gcctggccag ggctgtgtac aaggatgctg acctgtacct gctggactcc ccctttggct     4800

acctggatgt gctgacagag aaggagattt ttgagagctg tgtgtgcaag ctgatggcca     4860

acaagaccag aatcctggtg accagcaaga tggagcacct gaagaaggct gacaagatcc     4920

tgatcctgca tgagggcagc agctacttct atgggacctt ctctgagctg cagaacctgc     4980

agcctgactt cagctctaag ctgatgggct gtgacagctt tgaccagttc tctgctgaga     5040

ggaggaacag catcctgaca gagaccctgc acagattcag cctggaggga gatgcccctg     5100

tgagctggac agagaccaag aagcagagct tcaagcagac aggggagttt ggggagaaga     5160

ggaagaactc catcctgaac cccatcaaca gcatcaggaa gttcagcatt gtgcagaaaa     5220

cccccctgca gatgaatggc attgaggaag attctgatga gcccctggag aggagactga     5280

gcctggtgcc tgattctgag cagggagagg ccatcctgcc taggatctct gtgatcagca     5340

caggccctac actgcaggcc agaaggaggc agtctgtgct gaacctgatg acccactctg     5400

tgaaccaggg ccagaacatc cacaggaaaa ccacagcctc caccaggaaa gtgagcctgg     5460

cccctcaggc caatctgaca gagctggaca tctacagcag gaggctgtct caggagacag     5520

gcctggagat ttctgaggag atcaatgagg aggacctgaa agagtgcttc tttgatgaca     5580

tggagagcat ccctgctgtg accacctgga acacctacct gagatacatc acagtgcaca     5640

agagcctgat ctttgtgctg atctggtgcc tggtgatctt cctggctgaa gtggctgcct     5700

ctctggtggt gctgtggctg ctgggaaaca ccccactgca ggacaagggc aacagcaccc     5760

acagcaggaa caacagctat gctgtgatca tcacctccac ctccagctac tatgtgttct     5820

acatctatgt gggagtggct gataccctgc tggctatggg cttctttaga ggcctgcccc     5880

tggtgcacac actgatcaca gtgagcaaga tcctccacca caagatgctg cactctgtgc     5940

tgcaggctcc tatgagcacc ctgaataccc tgaaggctgg gggcatcctg aacagattct     6000

ccaaggatat tgccatcctg gatgacctgc tgcctctcac catctttgac ttcatccagc     6060

tgctgctgat tgtgattggg gccattgctg tggtggcagt gctgcagccc tacatctttg     6120

tggccacagt gcctgtgatt gtggccttca tcatgctgag ggcctacttt ctgcagacct     6180

cccagcagct gaagcagctg gagtctgagg gcagaagccc catcttcacc cacctggtga     6240

caagcctgaa gggcctgtgg accctgagag cctttggcag gcagccctac tttgagaccc     6300

tgttccacaa ggccctgaac ctgcacacag ccaactggtt cctctacctg tccaccctga     6360

gatggttcca gatgagaatt gagatgatct ttgtcatctt cttcattgct gtgaccttca     6420

tcagcattct gaccacagga gagggagagg gcagagtggg cattatcctg accctggcca     6480

tgaacatcat gagcacactg cagtgggcag tgaacagcag cattgatgtg gacagcctga     6540

tgaggagtgt gagcagagtg ttcaagttca ttgatatgcc cacagagggc aagcctacca     6600

agagcaccaa gccctacaag aatggccagc tgagcaaagt gatgatcatt gagaacagcc     6660

atgtgaagaa ggatgatatc tggcccagtg gaggccagat gacagtgaag gacctgacag     6720

ccaagtacac agaggggggc aatgctatcc tggagaacat ctccttcagc atctcccctg     6780

gccagagagt gggactgctg ggaagaacag gctctggcaa gtctaccctg ctgtctgcct     6840

tcctgaggct gctgaacaca gagggagaga tccagattga tggagtgtcc tgggacagca     6900

tcacactgca gcagtggagg aaggcctttg gtgtgatccc ccagaaagtg ttcatcttca     6960

gtggcacctt caggaagaac ctggacccct atgagcagtg gtctgaccag gagatttgga     7020

aagtggctga tgaagtgggc ctgagaagtg tgattgagca gttccctggc aagctggact     7080

ttgtcctggt ggatgggggc tgtgtgctga gccatggcca caagcagctg atgtgcctgg     7140

ccagatcagt gctgagcaag gccaagatcc tgctgctgga tgagccttct gcccacctgg     7200

atcctgtgac ctaccagatc atcaggagga ccctcaagca ggcctttgct gactgcacag     7260

tcatcctgtg tgagcacagg attgaggcca tgctggagtg ccagcagttc ctggtgattg     7320

aggagaacaa agtgaggcag tatgacagca tccagaagct gctgaatgag aggagcctgt     7380

tcaggcaggc catcagcccc tctgatagag tgaagctgtt cccccacagg aacagctcca     7440

agtgcaagag caagccccag attgctgccc tgaaggagga gacagaggag gaagtgcagg     7500

acaccaggct gtgagggccc aatcaacctc tggattacaa aatttgtgaa agattgactg     7560

gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt     7620

atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc     7680

tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt     7740

ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga     7800

ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct     7860

gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat     7920

cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct     7980

gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc     8040

tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg     8100

cctccccgca agcttcgcac tttttaaaag aaaagggagg actggatggg atttattact     8160

ccgataggac gctggcttgt aactcagtct cttactagga gaccagcttg agcctgggtg     8220

ttcgctggtt agcctaacct ggttggccac caggggtaag gactccttgg cttagaaagc     8280

taataaactt gcctgcatta gagctcttac gcgtcccggg ctcgagatcc gcatctcaat     8340

tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt     8400

tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc     8460

gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt     8520

tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca     8580

caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca     8640

tcaatgtatc ttatcatgtc tgtccgcttc ctcgctcact gactcgctgc gctcggtcgt     8700

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc     8760

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa     8820

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa     8880

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc     8940

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc     9000

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag     9060

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga     9120

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc     9180

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac     9240

agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg     9300

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca     9360

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa     9420

aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa     9480

ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt     9540

aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag     9600

ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat     9660

accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca     9720

taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc     9780

tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac     9840

tgaatccggt gagaatggca acagcttatg catttctttc cagacttgtt caacaggcca     9900

gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg     9960

cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga    10020

atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata    10080

ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta accatgcatc    10140

atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt    10200

tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa    10260

caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac    10320

attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg    10380

cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat    10440

gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca    10500

gagattttga gacacaacaa ttggtcgacg gatcc                               10535


<210>  2
<211>  9885
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM297 plasmid

<400>  2
gctcgagact agtgacttgg tgagtaggct tcgagcctag ttagaggact aggagaggcc       60

gtagccgtaa ctactctggg caagtagggc aggcggtggg tacgcaatgg gggcggctac      120

ctcagcacta aataggagac aattagacca atttgagaaa atacgacttc gcccgaacgg      180

aaagaaaaag taccaaatta aacatttaat atgggcaggc aaggagatgg agcgcttcgg      240

cctccatgag aggttgttgg agacagagga ggggtgtaaa agaatcatag aagtcctcta      300

ccccctagaa ccaacaggat cggagggctt aaaaagtctg ttcaatcttg tgtgcgtact      360

atattgcttg cacaaggaac agaaagtgaa agacacagag gaagcagtag caacagtaag      420

acaacactgc catctagtgg aaaaagaaaa aagtgcaaca gagacatcta gtggacaaaa      480

gaaaaatgac aagggaatag cagcgccacc tggtggcagt cagaattttc cagcgcaaca      540

acaaggaaat gcctgggtac atgtaccctt gtcaccgcgc accttaaatg cgtgggtaaa      600

agcagtagag gagaaaaaat ttggagcaga aatagtaccc atgtttcaag ccctatcaga      660

aggctgcaca ccctatgaca ttaatcagat gcttaatgtg ctaggagatc atcaaggggc      720

attacaaata gtgaaagaga tcattaatga agaagcagcc cagtgggatg taacacaccc      780

actacccgca ggacccctac cagcaggaca gctcagggac cctcgcggct cagatatagc      840

agggaccacc agctcagtac aagaacagtt agaatggatc tatactgcta acccccgggt      900

agatgtaggt gccatctacc ggagatggat tattctagga cttcaaaagt gtgtcaaaat      960

gtacaaccca gtatcagtcc tagacattag gcagggacct aaagagccct tcaaggatta     1020

tgtggacaga ttttacaagg caattagagc agaacaagcc tcaggggaag tgaaacaatg     1080

gatgacagaa tcattactca ttcaaaatgc taatccagat tgtaaggtca tcctgaaggg     1140

cctaggaatg caccccaccc ttgaagaaat gttaacggct tgtcaggggg taggaggccc     1200

aagctacaaa gcaaaagtaa tggcagaaat gatgcagacc atgcaaaatc aaaacatggt     1260

gcagcaggga ggtccaaaaa gacaaagacc cccactaaga tgttataatt gtggaaaatt     1320

tggccatatg caaagacaat gtccggaacc aaggaaaaca aaatgtctaa agtgtggaaa     1380

attgggacac ctagcaaaag actgcagggg acaggtgaat tttttagggt atggacggtg     1440

gatgggggca aaaccgagaa attttcccgc cgctactctt ggagcggaac cgagtgcgcc     1500

tcctccaccg agcggcacca ccccatacga cccagcaaag aagctcctgc agcaatatgc     1560

agagaaaggg aaacaactga gggagcaaaa gaggaatcca ccggcaatga atccggattg     1620

gaccgaggga tattctttga actccctctt tggagaagac caataaagac agtgtatata     1680

gaaggggtcc ccattaaggc actgctagac acaggggcag atgacaccat aattaaagaa     1740

aatgatttac aattatcagg tccatggaga cccaaaatta tagggggcat aggaggaggc     1800

cttaatgtaa aagaatataa cgacagggaa gtaaaaatag aagataaaat tttgagagga     1860

acaatattgt taggagcaac tcccattaat ataataggta gaaatttgct ggccccggca     1920

ggtgcccggt tagtaatggg acaattatca gaaaaaattc ctgtcacacc tgtcaaattg     1980

aaggaagggg ctcggggacc ctgtgtaaga caatggcctc tctctaaaga gaagattgaa     2040

gctttacagg aaatatgttc ccaattagag caggaaggaa aaatcagtag agtaggagga     2100

gaaaatgcat acaatacccc aatattttgc ataaagaaga aggacaaatc ccagtggagg     2160

atgctagtag actttagaga gttaaataag gcaacccaag atttctttga agtgcaatta     2220

gggatacccc acccagcagg attaagaaag atgagacaga taacagtttt agatgtagga     2280

gacgcctatt attccatacc attggatcca aattttagga aatatactgc ttttactatt     2340

cccacagtga ataatcaggg acccgggatt aggtatcaat tcaactgtct cccgcaaggg     2400

tggaaaggat ctcctacaat cttccaaaat acagcagcat ccattttgga ggagataaaa     2460

agaaacttgc cagcactaac cattgtacaa tacatggatg atttatgggt aggttctcaa     2520

gaaaatgaac acacccatga caaattagta gaacagttaa gaacaaaatt acaagcctgg     2580

ggcttagaaa ccccagaaaa gaaggtgcaa aaagaaccac cttatgagtg gatgggatac     2640

aaactttggc ctcacaaatg ggaactaagc agaatacaac tggaggaaaa agatgaatgg     2700

actgtcaatg acatccagaa gttagttggg aaactaaatt gggcagcaca attgtatcca     2760

ggtcttagga ccaagaatat atgcaagtta attagaggaa agaaaaatct gttagagcta     2820

gtgacttgga cacctgaggc agaagctgaa tatgcagaaa atgcagagat tcttaaaaca     2880

gaacaggaag gaacctatta caaaccagga atacctatta gggcagcagt acagaaattg     2940

gaaggaggac agtggagtta ccaattcaaa caagaaggac aagtcttgaa agtaggaaaa     3000

tacaccaagc aaaagaacac ccatacaaat gaacttcgca cattagctgg tttagtgcag     3060

aagatttgca aagaagctct agttatttgg gggatattac cagttctaga actcccgata     3120

gaaagagagg tatgggaaca atggtgggcg gattactggc aggtaagctg gattcccgaa     3180

tgggattttg tcagcacccc acctttgctc aaactatggt acacattaac aaaagaaccc     3240

atacccaagg aggacgttta ctatgtagat ggagcatgca acagaaattc aaaagaagga     3300

aaagcaggat acatctcaca atacggaaaa cagagagtag aaacattaga aaacactacc     3360

aatcagcaag cagaattaac agctataaaa atggctttgg aagacagtgg gcctaatgtg     3420

aacatagtaa cagactctca atatgcaatg ggaattttga cagcacaacc cacacaaagt     3480

gattcaccat tagtagagca aattatagcc ttaatgatac aaaagcaaca aatatatttg     3540

cagtgggtac cagcacataa aggaatagga ggaaatgagg agatagataa attagtgagt     3600

aaaggcatta gaagagtttt attcttagaa aaaatagaag aagctcaaga agagcatgaa     3660

agatatcata ataattggaa aaacctagca gatacatatg ggcttccaca aatagtagca     3720

aaagagatag tggccatgtg tccaaaatgt cagataaagg gagaaccagt gcatggacaa     3780

gtggatgcct cacctggaac atggcagatg gattgtactc atctagaagg aaaagtagtc     3840

atagttgcgg tccatgtagc cagtggattc atagaagcag aagtcatacc tagggaaaca     3900

ggaaaagaaa cggcaaagtt tctattaaaa atactgagta gatggcctat aacacagtta     3960

cacacagaca atgggcctaa ctttacctcc caagaagtgg cagcaatatg ttggtgggga     4020

aaaattgaac atacaacagg tataccatat aacccccaat ctcaaggatc aatagaaagc     4080

atgaacaaac aattaaaaga gataattggg aaaataagag atgattgcca atatacagag     4140

acagcagtac tgatggcttg ccatattcac aattttaaaa gaaagggagg aataggggga     4200

cagacttcag cagagagact aattaatata ataacaacac aattagaaat acaacattta     4260

caaaccaaaa ttcaaaaaat tttaaatttt agagtctact acagagaagg gagagaccct     4320

gtgtggaaag gaccagcaca attaatctgg aaaggggaag gagcagtggt cctcaaggac     4380

ggaagtgacc taaaggttgt accaagaagg aaagctaaaa ttattaagga ttatgaaccc     4440

aaacaaagag tgggtaatga gggtgacgtg gaaggtacca ggggatctga taactaaatg     4500

gcagggaata gtcagatatt ggatgagaca aagaaatttg aaatggaact attatatgca     4560

tcagctggcg gccgcgaatt cactagtgat tcccgtttgt gctagggttc ttaggcttct     4620

tgggggctgc tggaactgca atgggagcag cggcgacagc cctgacggtc cagtctcagc     4680

atttgcttgc tgggatactg cagcagcaga agaatctgct ggcggctgtg gaggctcaac     4740

agcagatgtt gaagctgacc atttggggtg ttaaaaacct caatgcccgc gtcacagccc     4800

ttgagaagta cctagaggat caggcacgac taaactcctg ggggtgcgca tggaaacaag     4860

tatgtcatac cacagtggag tggccctgga caaatcggac tccggattgg caaaatatga     4920

cttggttgga gtgggaaaga caaatagctg atttggaaag caacattacg agacaattag     4980

tgaaggctag agaacaagag gaaaagaatc tagatgccta tcagaagtta actagttggt     5040

cagatttctg gtcttggttc gatttctcaa aatggcttaa cattttaaaa atgggatttt     5100

tagtaatagt aggaataata gggttaagat tactttacac agtatatgga tgtatagtga     5160

gggttaggca gggatatgtt cctctatctc cacagatcca tatccaatcg aattcccgcg     5220

gccgcaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa     5280

tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat     5340

catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat     5400

agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt     5460

aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc     5520

catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca     5580

ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt     5640

gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt     5700

cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga     5760

cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc     5820

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct     5880

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa     5940

acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac     6000

tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact     6060

aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta     6120

gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct     6180

tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca     6240

ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtccgctt     6300

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     6360

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     6420

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     6480

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     6540

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     6600

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     6660

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     6720

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     6780

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     6840

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     6900

gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     6960

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     7020

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     7080

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat     7140

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct     7200

aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa     7260

actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta     7320

atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg     7380

cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt     7440

tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aacagcttat     7500

gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg     7560

catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc     7620

tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg     7680

catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgtttttc     7740

cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg     7800

tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat     7860

tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca     7920

atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata     7980

aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat     8040

ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg     8100

atatattttt atcttgtgca atgtaacatc agagattttg agacacaaca attgtcgaca     8160

ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata     8220

tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga     8280

cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt     8340

ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt     8400

gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca     8460

ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt     8520

catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc     8580

cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg     8640

ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg     8700

ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt     8760

tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt     8820

cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg     8880

gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg     8940

ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct     9000

tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg     9060

tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg     9120

gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg     9180

tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt     9240

gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc     9300

tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc     9360

ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc     9420

gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg     9480

tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg     9540

acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc     9600

tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct     9660

tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg     9720

ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg     9780

cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg     9840

caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaatt                     9885


<210>  3
<211>  3384
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM299 plasmid

<400>  3
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc      660

cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc      720

tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat      780

tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc      840

gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa      900

actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac      960

tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta     1020

aggctagagt acttaatacg actcactata ggctagcctc gagaattcga ttatgcccct     1080

aggaccagaa gaaagaagat tgcttcgctt gatttggctc ctttacagca ccaatccata     1140

tccaccaagt ggggaaggga cggccagaca acgccgacga gccaggagaa ggtggagaca     1200

acagcaggat caaattagag tcttggtaga aagactccaa gagcaggtgt atgcagttga     1260

ccgcctggct gacgaggctc aacacttggc tatacaacag ttgcctgacc ctcctcattc     1320

agcttagaat cactagtgaa ttcacgcgtg gtacctctag agtcgacccg ggcggccgct     1380

tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg     1440

aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag     1500

ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga     1560

gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga     1620

tccgtcgacc aattgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata     1680

tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga     1740

gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg     1800

atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc     1860

gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg     1920

ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc     1980

cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc     2040

ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg     2100

atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta     2160

acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg     2220

atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa     2280

tgcataagct gttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg     2340

ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa     2400

tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt     2460

cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc     2520

agtttcattt gatgctcgat gagtttttct aactgtcaga ccaagtttac tcatatatac     2580

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     2640

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     2700

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     2760

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     2820

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt     2880

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     2940

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     3000

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     3060

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     3120

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     3180

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     3240

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     3300

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     3360

ttgctcacat ggctcgacag atct                                            3384


<210>  4
<211>  6264
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM301 plasmid

<400>  4
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat       60

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg      120

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt      180

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag      240

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc      300

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag      360

tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc      420

ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg      480

gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcaag gggcggggcg      540

gggcgaggcg gaaaggtgcg gcggcagcca atcaaagcgg cgcgctccga aagtttcctt      600

ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag      660

tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc      720

ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg      780

gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc      840

ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt      900

gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg      960

ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg     1020

gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg     1080

tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc     1140

ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg     1200

cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc     1260

cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct     1320

gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg     1380

gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct     1440

ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc     1500

ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg     1560

gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg     1620

gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg     1680

gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcgat tgccatggca     1740

acatatatcc agagagtaca gtgcatctca acatcactac tggttgttct caccacattg     1800

gtctcgtgtc agattcccag ggataggctc tctaacatag gggtcatagt cgatgaaggg     1860

aaatcactga agatagctgg atcccacgaa tcgaggtaca tagtactgag tctagttccg     1920

ggggtagact ttgagaatgg gtgcggaaca gcccaggtta tccagtacaa gagcctactg     1980

aacaggctgt taatcccatt gagggatgcc ttagatcttc aggaggctct gataactgtc     2040

accaatgata cgacacaaaa tgccggtgct ccccagtcga gattcttcgg tgctgtgatt     2100

ggtactatcg cacttggagt ggcgacatca gcacaaatca ccgcagggat tgcactagcc     2160

gaagcgaggg aggccaaaag agacatagcg ctcatcaaag aatcgatgac aaaaacacac     2220

aagtctatag aactgctgca aaacgctgtg ggggaacaaa ttcttgctct aaagacactc     2280

caggatttcg tgaatgatga gatcaaaccc gcaataagcg aattaggctg tgagactgct     2340

gccttaagac tgggtataaa attgacacag cattactccg agctgttaac tgcgttcggc     2400

tcgaatttcg gaaccatcgg agagaagagc ctcacgctgc aggcgctgtc ttcactttac     2460

tctgctaaca ttactgagat tatgaccaca atcaggacag ggcagtctaa catctatgat     2520

gtcatttata cagaacagat caaaggaacg gtgatagatg tggatctaga gagatacatg     2580

gtcaccctgt ctgtgaagat ccctattctt tctgaagtcc caggtgtgct catacacaag     2640

gcatcatcta tttcttacaa catagacggg gaggaatggt atgtgactgt ccccagccat     2700

atactcagtc gtgcttcttt cttagggggt gcagacataa ccgattgtgt tgagtccaga     2760

ttgacctata tatgccccag ggatcccgca caactgatac ctgacagcca gcaaaagtgt     2820

atcctggggg acacaacaag gtgtcctgtc acaaaagttg tggacagcct tatccccaag     2880

tttgcttttg tgaatggggg cgttgttgct aactgcatag catccacatg tacctgcggg     2940

acaggccgaa gaccaatcag tcaggatcgc tctaaaggtg tagtattcct aacccatgac     3000

aactgtggtc ttataggtgt caatggggta gaattgtatg ctaaccggag agggcacgat     3060

gccacttggg gggtccagaa cttgacagtc ggtcctgcaa ttgctatcag acccgttgat     3120

atttctctca accttgctga tgctacgaat ttcttgcaag actctaaggc tgagcttgag     3180

aaagcacgga aaatcctctc ggaggtaggt agatggtaca actcaagaga gactgtgatt     3240

acgatcatag tagttatggt cgtaatattg gtggtcatta tagtgatcat catcgtgctt     3300

tatagactca gaaggtgaaa tcactagtga attcactcct caggtgcagg ctgcctatca     3360

gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc     3420

ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata     3480

aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag     3540

gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc     3600

aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta     3660

tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta     3720

gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt     3780

acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc     3840

tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc     3900

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca     3960

taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct     4020

cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc     4080

agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc     4140

ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc     4200

ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa     4260

aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat     4320

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat     4380

gtatcttatc atgtctgtcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     4440

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     4500

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     4560

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     4620

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     4680

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     4740

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     4800

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     4860

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     4920

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     4980

tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc     5040

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     5100

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     5160

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     5220

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     5280

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttaga     5340

aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat     5400

atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga     5460

tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta     5520

atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat     5580

ccggtgagaa tggcaacagc ttatgcattt ctttccagac ttgttcaaca ggccagccat     5640

tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct     5700

gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca     5760

accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt     5820

ctaatacctg gaatgctgtt tttccgggga tcgcagtggt gagtaaccat gcatcatcag     5880

gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc     5940

tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact     6000

ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat     6060

cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag     6120

agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag     6180

cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat     6240

tttgagacac aacaattggt cgac                                            6264


<210>  5
<211>  6522
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM303 plasmid

<400>  5
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat       60

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg      120

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt      180

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag      240

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc      300

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag      360

tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc      420

ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg      480

gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg      540

gggcgaggcg gaaaggtgcg gcggcagcca atcaaagcgg cgcgctccga aagtttcctt      600

ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag      660

tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc      720

ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg      780

gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc      840

ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt      900

gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg      960

ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg     1020

gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg     1080

tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc     1140

ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg     1200

cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc     1260

cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct     1320

gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg     1380

gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct     1440

ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc     1500

ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg     1560

gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc     1620

taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt     1680

gctgtctcat cattttggca aagaattcct cgagcatgtg gtctgagtta aaaatcagga     1740

gcaacgacgg aggtgaagga ccagaggacg ccaacgaccc ccggggaaag ggggtgcaac     1800

acatccatat ccagccatct ctacctgttt atggacagag ggttagggat ggtgataggg     1860

gcaaacgtga ctcgtactgg tctacttctc ctagtggtag caccacaaaa ccagcatcag     1920

gttgggagag gtcaagtaaa gccgacacat ggttgctgat tctctcattc acccagtggg     1980

ctttgtcaat tgccacagtg atcatctgta tcataatttc tgctagacaa gggtatagta     2040

tgaaagagta ctcaatgact gtagaggcat tgaacatgag cagcagggag gtgaaagagt     2100

cacttaccag tctaataagg caagaggtta tagcaagggc tgtcaacatt cagagctctg     2160

tgcaaaccgg aatcccagtc ttgttgaaca aaaacagcag ggatgtcatc cagatgattg     2220

ataagtcgtg cagcagacaa gagctcactc agcactgtga gagtacgatc gcagtccacc     2280

atgccgatgg aattgcccca cttgagccac atagtttctg gagatgccct gtcggagaac     2340

cgtatcttag ctcagatcct gaaatctcat tgctgcctgg tccgagcttg ttatctggtt     2400

ctacaacgat ctctggatgt gttaggctcc cttcactctc aattggcgag gcaatctatg     2460

cctattcatc aaatctcatt acacaaggtt gtgctgacat agggaaatca tatcaggtcc     2520

tgcagctagg gtacatatca ctcaattcag atatgttccc tgatcttaac cccgtagtgt     2580

cccacactta tgacatcaac gacaatcgga aatcatgctc tgtggtggca accgggacta     2640

ggggttatca gctttgctcc atgccgactg tagacgaaag aaccgactac tctagtgatg     2700

gtattgagga tctggtcctt gatgtcctgg atctcaaagg gagaactaag tctcaccggt     2760

atcgcaacag cgaggtagat cttgatcacc cgttctctgc actatacccc agtgtaggca     2820

acggcattgc aacagaaggc tcattgatat ttcttgggta tggtggacta accacccctc     2880

tgcagggtga tacaaaatgt aggacccaag gatgccaaca ggtgtcgcaa gacacatgca     2940

atgaggctct gaaaattaca tggctaggag ggaaacaggt ggtcagcgtg atcatccagg     3000

tcaatgacta tctctcagag aggccaaaga taagagtcac aaccattcca atcactcaaa     3060

actatctcgg ggcggaaggt agattattaa aattgggtga tcgggtgtac atctatacaa     3120

gatcatcagg ctggcactct caactgcaga taggagtact tgatgtcagc caccctttga     3180

ctatcaactg gacacctcat gaagccttgt ctagaccagg aaataaagag tgcaattggt     3240

acaataagtg tccgaaggaa tgcatatcag gcgtatacac tgatgcttat ccattgtccc     3300

ctgatgcagc taacgtcgct accgtcacgc tatatgccaa tacatcgcgt gtcaacccaa     3360

caatcatgta ttctaacact actaacatta taaatatgtt aaggataaag gatgttcaat     3420

tagaggctgc atataccacg acatcgtgta tcacgcattt tggtaaaggc tactgctttc     3480

acatcatcga gatcaatcag aagagcctga ataccttaca gccgatgctc tttaagacta     3540

gcatccctaa attatgcaag gccgagtctt aagcggccgc gcatgcgaat tcactcctca     3600

ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata     3660

ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca     3720

tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt     3780

gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta     3840

tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct     3900

ataaagaggt catcagtata tgaaacagcc ccctgctgtc tattccttat tccatagaaa     3960

agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac     4020

atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact     4080

cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg     4140

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca     4200

acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca     4260

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga     4320

tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct     4380

aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc     4440

agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg     4500

aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa     4560

agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt     4620

ttgtccaaac tcatcaatgt atcttatcat gtctgtccgc ttcctcgctc actgactcgc     4680

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt     4740

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg     4800

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     4860

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     4920

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     4980

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     5040

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     5100

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa     5160

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg     5220

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag     5280

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt     5340

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     5400

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc     5460

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca     5520

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa     5580

cttggtctga cagttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat     5640

caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac     5700

cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa     5760

catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac     5820

catgagtgac gactgaatcc ggtgagaatg gcaacagctt atgcatttct ttccagactt     5880

gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat     5940

tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac     6000

aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac     6060

ctgaatcagg atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga     6120

gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt     6180

ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc     6240

catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac     6300

ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg     6360

aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg     6420

tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg     6480

caatgtaaca tcagagattt tgagacacaa caattggtcg ac                        6522


<210>  6
<211>  574
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCEF promoter

<400>  6
agatctgtta cataacttat ggtaaatggc ctgcctggct gactgcccaa tgacccctgc       60

ccaatgatgt caataatgat gtatgttccc atgtaatgcc aatagggact ttccattgat      120

gtcaatgggt ggagtattta tggtaactgc ccacttggca gtacatcaag tgtatcatat      180

gccaagtatg ccccctattg atgtcaatga tggtaaatgg cctgcctggc attatgccca      240

gtacatgacc ttatgggact ttcctacttg gcagtacatc tatgtattag tcattgctat      300

taccatggga attcactagt ggagaagagc atgcttgagg gctgagtgcc cctcagtggg      360

cagagagcac atggcccaca gtccctgaga agttgggggg aggggtgggc aattgaactg      420

gtgcctagag aaggtggggc ttgggtaaac tgggaaagtg atgtggtgta ctggctccac      480

ctttttcccc agggtggggg agaaccatat ataagtgcag tagtctctgt gaacattcaa      540

gcttctgcct tctccctcct gtgagtttgc tagc                                  574


<210>  7
<211>  4459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimised CpG depleted CFTR transgene

<400>  7
gctagccacc atgcagagaa gccctctgga gaaggcctct gtggtgagca agctgttctt       60

cagctggacc aggcccatcc tgaggaaggg ctacaggcag agactggagc tgtctgacat      120

ctaccagatc ccctctgtgg actctgctga caacctgtct gagaagctgg agagggagtg      180

ggatagagag ctggccagca agaagaaccc caagctgatc aatgccctga ggagatgctt      240

cttctggaga ttcatgttct atggcatctt cctgtacctg ggggaagtga ccaaggctgt      300

gcagcctctg ctgctgggca gaatcattgc cagctatgac cctgacaaca aggaggagag      360

gagcattgcc atctacctgg gcattggcct gtgcctgctg ttcattgtga ggaccctgct      420

gctgcaccct gccatctttg gcctgcacca cattggcatg cagatgagga ttgccatgtt      480

cagcctgatc tacaagaaaa ccctgaagct gtccagcaga gtgctggaca agatcagcat      540

tggccagctg gtgagcctgc tgagcaacaa cctgaacaag tttgatgagg gcctggccct      600

ggcccacttt gtgtggattg cccctctgca ggtggccctg ctgatgggcc tgatttggga      660

gctgctgcag gcctctgcct tttgtggcct gggcttcctg attgtgctgg ccctgtttca      720

ggctggcctg ggcaggatga tgatgaagta cagggaccag agggcaggca agatcagtga      780

gaggctggtg atcacctctg agatgattga gaacatccag tctgtgaagg cctactgttg      840

ggaggaagct atggagaaga tgattgaaaa cctgaggcag acagagctga agctgaccag      900

gaaggctgcc tatgtgagat acttcaacag ctctgccttc ttcttctctg gcttctttgt      960

ggtgttcctg tctgtgctgc cctatgccct gatcaagggg atcatcctga gaaagatttt     1020

caccaccatc agcttctgca ttgtgctgag gatggctgtg accagacagt tcccctgggc     1080

tgtgcagacc tggtatgaca gcctgggggc catcaacaag atccaggact tcctgcagaa     1140

gcaggagtac aagaccctgg agtacaacct gaccaccaca gaagtggtga tggagaatgt     1200

gacagccttc tgggaggagg gctttgggga gctgtttgag aaggccaagc agaacaacaa     1260

caacagaaag accagcaatg gggatgactc cctgttcttc tccaacttct ccctgctggg     1320

cacacctgtg ctgaaggaca tcaacttcaa gattgagagg gggcagctgc tggctgtggc     1380

tggatctaca ggggctggca agaccagcct gctgatgatg atcatggggg agctggagcc     1440

ttctgagggc aagatcaagc actctggcag gatcagcttt tgcagccagt tcagctggat     1500

catgcctggc accatcaagg agaacatcat ctttggagtg agctatgatg agtacagata     1560

caggagtgtg atcaaggcct gccagctgga ggaggacatc agcaagtttg ctgagaagga     1620

caacattgtg ctgggggagg gaggcattac actgtctggg ggccagagag ccagaatcag     1680

cctggccagg gctgtgtaca aggatgctga cctgtacctg ctggactccc cctttggcta     1740

cctggatgtg ctgacagaga aggagatttt tgagagctgt gtgtgcaagc tgatggccaa     1800

caagaccaga atcctggtga ccagcaagat ggagcacctg aagaaggctg acaagatcct     1860

gatcctgcat gagggcagca gctacttcta tgggaccttc tctgagctgc agaacctgca     1920

gcctgacttc agctctaagc tgatgggctg tgacagcttt gaccagttct ctgctgagag     1980

gaggaacagc atcctgacag agaccctgca cagattcagc ctggagggag atgcccctgt     2040

gagctggaca gagaccaaga agcagagctt caagcagaca ggggagtttg gggagaagag     2100

gaagaactcc atcctgaacc ccatcaacag catcaggaag ttcagcattg tgcagaaaac     2160

ccccctgcag atgaatggca ttgaggaaga ttctgatgag cccctggaga ggagactgag     2220

cctggtgcct gattctgagc agggagaggc catcctgcct aggatctctg tgatcagcac     2280

aggccctaca ctgcaggcca gaaggaggca gtctgtgctg aacctgatga cccactctgt     2340

gaaccagggc cagaacatcc acaggaaaac cacagcctcc accaggaaag tgagcctggc     2400

ccctcaggcc aatctgacag agctggacat ctacagcagg aggctgtctc aggagacagg     2460

cctggagatt tctgaggaga tcaatgagga ggacctgaaa gagtgcttct ttgatgacat     2520

ggagagcatc cctgctgtga ccacctggaa cacctacctg agatacatca cagtgcacaa     2580

gagcctgatc tttgtgctga tctggtgcct ggtgatcttc ctggctgaag tggctgcctc     2640

tctggtggtg ctgtggctgc tgggaaacac cccactgcag gacaagggca acagcaccca     2700

cagcaggaac aacagctatg ctgtgatcat cacctccacc tccagctact atgtgttcta     2760

catctatgtg ggagtggctg ataccctgct ggctatgggc ttctttagag gcctgcccct     2820

ggtgcacaca ctgatcacag tgagcaagat cctccaccac aagatgctgc actctgtgct     2880

gcaggctcct atgagcaccc tgaataccct gaaggctggg ggcatcctga acagattctc     2940

caaggatatt gccatcctgg atgacctgct gcctctcacc atctttgact tcatccagct     3000

gctgctgatt gtgattgggg ccattgctgt ggtggcagtg ctgcagccct acatctttgt     3060

ggccacagtg cctgtgattg tggccttcat catgctgagg gcctactttc tgcagacctc     3120

ccagcagctg aagcagctgg agtctgaggg cagaagcccc atcttcaccc acctggtgac     3180

aagcctgaag ggcctgtgga ccctgagagc ctttggcagg cagccctact ttgagaccct     3240

gttccacaag gccctgaacc tgcacacagc caactggttc ctctacctgt ccaccctgag     3300

atggttccag atgagaattg agatgatctt tgtcatcttc ttcattgctg tgaccttcat     3360

cagcattctg accacaggag agggagaggg cagagtgggc attatcctga ccctggccat     3420

gaacatcatg agcacactgc agtgggcagt gaacagcagc attgatgtgg acagcctgat     3480

gaggagtgtg agcagagtgt tcaagttcat tgatatgccc acagagggca agcctaccaa     3540

gagcaccaag ccctacaaga atggccagct gagcaaagtg atgatcattg agaacagcca     3600

tgtgaagaag gatgatatct ggcccagtgg aggccagatg acagtgaagg acctgacagc     3660

caagtacaca gaggggggca atgctatcct ggagaacatc tccttcagca tctcccctgg     3720

ccagagagtg ggactgctgg gaagaacagg ctctggcaag tctaccctgc tgtctgcctt     3780

cctgaggctg ctgaacacag agggagagat ccagattgat ggagtgtcct gggacagcat     3840

cacactgcag cagtggagga aggcctttgg tgtgatcccc cagaaagtgt tcatcttcag     3900

tggcaccttc aggaagaacc tggaccccta tgagcagtgg tctgaccagg agatttggaa     3960

agtggctgat gaagtgggcc tgagaagtgt gattgagcag ttccctggca agctggactt     4020

tgtcctggtg gatgggggct gtgtgctgag ccatggccac aagcagctga tgtgcctggc     4080

cagatcagtg ctgagcaagg ccaagatcct gctgctggat gagccttctg cccacctgga     4140

tcctgtgacc taccagatca tcaggaggac cctcaagcag gcctttgctg actgcacagt     4200

catcctgtgt gagcacagga ttgaggccat gctggagtgc cagcagttcc tggtgattga     4260

ggagaacaaa gtgaggcagt atgacagcat ccagaagctg ctgaatgaga ggagcctgtt     4320

caggcaggcc atcagcccct ctgatagagt gaagctgttc ccccacagga acagctccaa     4380

gtgcaagagc aagccccaga ttgctgccct gaaggaggag acagaggagg aagtgcagga     4440

caccaggctg tgagggccc                                                  4459


<210>  8
<211>  600
<212>  DNA
<213>  Woodchuck hepatitis virus

<400>  8
gggcccaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat       60

gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct      120

tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag      180

gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc      240

cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc      300

ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct      360

cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg      420

ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg      480

gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg      540

cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcaagct      600


<210>  9
<211>  7349
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM407 plasmid

<400>  9
ggtacctcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat       60

tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc      120

atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat      180

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      240

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      300

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      360

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt      420

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc      480

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      540

gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      600

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      660

caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc      720

tatataagca gagctcgctg gcttgtaact cagtctctta ctaggagacc agcttgagcc      780

tgggtgttcg ctggttagcc taacctggtt ggccaccagg ggtaaggact ccttggctta      840

gaaagctaat aaacttgcct gcattagagc ttatctgagt caagtgtcct cattgacgcc      900

tcactctctt gaacgggaat cttccttact gggttctctc tctgacccag gcgagagaaa      960

ctccagcagt ggcgcccgaa cagggacttg agtgagagtg taggcacgta cagctgagaa     1020

ggcgtcggac gcgaaggaag cgcggggtgc gacgcgacca agaaggagac ttggtgagta     1080

ggcttctcga gtgccgggaa aaagctcgag cctagttaga ggactaggag aggccgtagc     1140

cgtaactact cttgggcaag tagggcaggc ggtgggtacg caatgggggc ggctacctca     1200

gcactaaata ggagacaatt agaccaattt gagaaaatac gacttcgccc gaacggaaag     1260

aaaaagtacc aaattaaaca tttaatatgg gcaggcaagg agatggagcg cttcggcctc     1320

catgagaggt tgttggagac agaggagggg tgtaaaagaa tcatagaagt cctctacccc     1380

ctagaaccaa caggatcgga gggcttaaaa agtctgttca atcttgtgtg cgtgctatat     1440

tgcttgcaca aggaacagaa agtgaaagac acagaggaag cagtagcaac agtaagacaa     1500

cactgccatc tagtggaaaa agaaaaaagt gcaacagaga catctagtgg acaaaagaaa     1560

aatgacaagg gaatagcagc gccacctggt ggcagtcaga attttccagc gcaacaacaa     1620

ggaaatgcct gggtacatgt acccttgtca ccgcgcacct taaatgcgtg ggtaaaagca     1680

gtagaggaga aaaaatttgg agcagaaata gtacccattt ttttgtttca agccctatcg     1740

aattcccgtt tgtgctaggg ttcttaggct tcttgggggc tgctggaact gcaatgggag     1800

cagcggcgac agccctgacg gtccagtctc agcatttgct tgctgggata ctgcagcagc     1860

agaagaatct gctggcggct gtggaggctc aacagcagat gttgaagctg accatttggg     1920

gtgttaaaaa cctcaatgcc cgcgtcacag cccttgagaa gtacctagag gatcaggcac     1980

gactaaactc ctgggggtgc gcatggaaac aagtatgtca taccacagtg gagtggccct     2040

ggacaaatcg gactccggat tggcaaaata tgacttggtt ggagtgggaa agacaaatag     2100

ctgatttgga aagcaacatt acgagacaat tagtgaaggc tagagaacaa gaggaaaaga     2160

atctagatgc ctatcagaag ttaactagtt ggtcagattt ctggtcttgg ttcgatttct     2220

caaaatggct taacatttta aaaatgggat ttttagtaat agtaggaata atagggttaa     2280

gattacttta cacagtatat ggatgtatag tgagggttag gcagggatat gttcctctat     2340

ctccacagat ccatatccgc ggcaatttta aaagaaaggg aggaataggg ggacagactt     2400

cagcagagag actaattaat ataataacaa cacaattaga aatacaacat ttacaaacca     2460

aaattcaaaa aattttaaat tttagagccg cggagatctg ttacataact tatggtaaat     2520

ggcctgcctg gctgactgcc caatgacccc tgcccaatga tgtcaataat gatgtatgtt     2580

cccatgtaat gccaataggg actttccatt gatgtcaatg ggtggagtat ttatggtaac     2640

tgcccacttg gcagtacatc aagtgtatca tatgccaagt atgcccccta ttgatgtcaa     2700

tgatggtaaa tggcctgcct ggcattatgc ccagtacatg accttatggg actttcctac     2760

ttggcagtac atctatgtat tagtcattgc tattaccatg ggaattcact agtggagaag     2820

agcatgcttg agggctgagt gcccctcagt gggcagagag cacatggccc acagtccctg     2880

agaagttggg gggaggggtg ggcaattgaa ctggtgccta gagaaggtgg ggcttgggta     2940

aactgggaaa gtgatgtggt gtactggctc cacctttttc cccagggtgg gggagaacca     3000

tatataagtg cagtagtctc tgtgaacatt caagcttctg ccttctccct cctgtgagtt     3060

tgctagccac catgcccagc tctgtgtcct ggggcattct gctgctggct ggcctgtgct     3120

gtctggtgcc tgtgtccctg gctgaggacc ctcaggggga tgctgcccag aaaacagaca     3180

cctcccacca tgaccaggac caccccacct tcaacaagat cacccccaac ctggcagagt     3240

ttgccttcag cctgtacaga cagctggccc accagagcaa cagcaccaac atctttttca     3300

gccctgtgtc cattgccaca gcctttgcca tgctgagcct gggcaccaag gctgacaccc     3360

atgatgagat cctggaaggc ctgaacttca acctgacaga gatccctgag gcccagatcc     3420

atgagggctt ccaggaactg ctgagaaccc tgaaccagcc agacagccag ctgcagctga     3480

caacaggcaa tgggctgttc ctgtctgagg gcctgaagct ggtggacaag tttctggaag     3540

atgtgaagaa gctgtaccac tctgaggcct tcacagtgaa ctttggggac acagaagagg     3600

ccaagaaaca gatcaatgac tatgtggaaa agggcaccca gggcaagatt gtggaccttg     3660

tgaaagagct ggacagggac actgtgtttg cccttgtgaa ctacatcttc ttcaagggca     3720

agtgggagag gccctttgaa gtgaaggaca ctgaggaaga ggacttccat gtggaccaag     3780

tgaccacagt gaaggtgcca atgatgaaga gactggggat gttcaatatc cagcactgca     3840

agaaactgag cagctgggtg ctgctgatga agtacctggg caatgctaca gccatattct     3900

ttctgcctga tgagggcaag ctgcagcacc tggaaaatga gctgacccat gacatcatca     3960

ccaaatttct ggaaaatgag gacagaagat ctgccagcct gcatctgccc aagctgagca     4020

tcacaggcac atatgacctg aagtctgtgc tgggacagct gggaatcacc aaggtgttca     4080

gcaatggggc agacctgagt ggagtgacag aggaagcccc tctgaagctg tccaaggctg     4140

tgcacaaggc agtgctgacc attgatgaga agggcacaga ggctgctggg gccatgtttc     4200

tggaagccat ccccatgtcc atccccccag aagtgaagtt caacaagccc tttgtgttcc     4260

tgatgattga gcagaacacc aagagccccc tgttcatggg caaggttgtg aaccccaccc     4320

agaaatgagg gcccaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc     4380

ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg     4440

ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc     4500

tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg     4560

acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg     4620

ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga     4680

caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct     4740

ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg     4800

tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc     4860

ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc     4920

cgcaagcttc gcacttttta aaagaaaagg gaggactgga tgggatttat tactccgata     4980

ggacgctggc ttgtaactca gtctcttact aggagaccag cttgagcctg ggtgttcgct     5040

ggttagccta acctggttgg ccaccagggg taaggactcc ttggcttaga aagctaataa     5100

acttgcctgc attagagctc ttacgcgtcc cgggctcgag atccgcatct caattagtca     5160

gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc     5220

cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg     5280

gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa     5340

aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt     5400

tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg     5460

tatcttatca tgtctgtccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     5520

gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     5580

taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc     5640

cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg     5700

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     5760

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     5820

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     5880

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     5940

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     6000

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     6060

cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct     6120

gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac     6180

cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc     6240

tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg     6300

ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta     6360

aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa     6420

aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata     6480

tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat     6540

ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa     6600

tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc     6660

cggtgagaat ggcaacagct tatgcatttc tttccagact tgttcaacag gccagccatt     6720

acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg     6780

agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa     6840

ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc     6900

taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg     6960

agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct     7020

gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc     7080

tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc     7140

gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga     7200

gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc     7260

agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt     7320

ttgagacaca acaattggtc gacggatcc                                       7349


<210>  10
<211>  6653
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM358 plasmid

<400>  10
ggtacctcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat       60

tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc      120

atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat      180

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      240

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      300

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      360

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt      420

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc      480

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      540

gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      600

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      660

caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc      720

tatataagca gagctcgctg gcttgtaact cagtctctta ctaggagacc agcttgagcc      780

tgggtgttcg ctggttagcc taacctggtt ggccaccagg ggtaaggact ccttggctta      840

gaaagctaat aaacttgcct gcattagagc ttatctgagt caagtgtcct cattgacgcc      900

tcactctctt gaacgggaat cttccttact gggttctctc tctgacccag gcgagagaaa      960

ctccagcagt ggcgcccgaa cagggacttg agtgagagtg taggcacgta cagctgagaa     1020

ggcgtcggac gcgaaggaag cgcggggtgc gacgcgacca agaaggagac ttggtgagta     1080

ggcttctcga gtgccgggaa aaagctcgag cctagttaga ggactaggag aggccgtagc     1140

cgtaactact cttgggcaag tagggcaggc ggtgggtacg caatgggggc ggctacctca     1200

gcactaaata ggagacaatt agaccaattt gagaaaatac gacttcgccc gaacggaaag     1260

aaaaagtacc aaattaaaca tttaatatgg gcaggcaagg agatggagcg cttcggcctc     1320

catgagaggt tgttggagac agaggagggg tgtaaaagaa tcatagaagt cctctacccc     1380

ctagaaccaa caggatcgga gggcttaaaa agtctgttca atcttgtgtg cgtgctatat     1440

tgcttgcaca aggaacagaa agtgaaagac acagaggaag cagtagcaac agtaagacaa     1500

cactgccatc tagtggaaaa agaaaaaagt gcaacagaga catctagtgg acaaaagaaa     1560

aatgacaagg gaatagcagc gccacctggt ggcagtcaga attttccagc gcaacaacaa     1620

ggaaatgcct gggtacatgt acccttgtca ccgcgcacct taaatgcgtg ggtaaaagca     1680

gtagaggaga aaaaatttgg agcagaaata gtacccattt ttttgtttca agccctatcg     1740

aattcccgtt tgtgctaggg ttcttaggct tcttgggggc tgctggaact gcaatgggag     1800

cagcggcgac agccctgacg gtccagtctc agcatttgct tgctgggata ctgcagcagc     1860

agaagaatct gctggcggct gtggaggctc aacagcagat gttgaagctg accatttggg     1920

gtgttaaaaa cctcaatgcc cgcgtcacag cccttgagaa gtacctagag gatcaggcac     1980

gactaaactc ctgggggtgc gcatggaaac aagtatgtca taccacagtg gagtggccct     2040

ggacaaatcg gactccggat tggcaaaata tgacttggtt ggagtgggaa agacaaatag     2100

ctgatttgga aagcaacatt acgagacaat tagtgaaggc tagagaacaa gaggaaaaga     2160

atctagatgc ctatcagaag ttaactagtt ggtcagattt ctggtcttgg ttcgatttct     2220

caaaatggct taacatttta aaaatgggat ttttagtaat agtaggaata atagggttaa     2280

gattacttta cacagtatat ggatgtatag tgagggttag gcagggatat gttcctctat     2340

ctccacagat ccatatccgc ggcaatttta aaagaaaggg aggaataggg ggacagactt     2400

cagcagagag actaattaat ataataacaa cacaattaga aatacaacat ttacaaacca     2460

aaattcaaaa aattttaaat tttagagccg cggagatctg ttacataact tatggtaaat     2520

ggcctgcctg gctgactgcc caatgacccc tgcccaatga tgtcaataat gatgtatgtt     2580

cccatgtaat gccaataggg actttccatt gatgtcaatg ggtggagtat ttatggtaac     2640

tgcccacttg gcagtacatc aagtgtatca tatgccaagt atgcccccta ttgatgtcaa     2700

tgatggtaaa tggcctgcct ggcattatgc ccagtacatg accttatggg actttcctac     2760

ttggcagtac atctatgtat tagtcattgc tattaccatg ggaattcact agtggagaag     2820

agcatgcttg agggctgagt gcccctcagt gggcagagag cacatggccc acagtccctg     2880

agaagttggg gggaggggtg ggcaattgaa ctggtgccta gagaaggtgg ggcttgggta     2940

aactgggaaa gtgatgtggt gtactggctc cacctttttc cccagggtgg gggagaacca     3000

tatataagtg cagtagtctc tgtgaacatt caagcttctg ccttctccct cctgtgagtt     3060

tgctagccac catgggagtg aaggtgctgt ttgccctgat ctgcattgct gtggctgagg     3120

ccaagcccac agagaacaat gaggacttca acattgtggc tgtggccagc aactttgcca     3180

ccacagacct ggatgctgac aggggcaagc tgcctggcaa gaagctgccc ctggaagtcc     3240

tgaaagagat ggaagccaat gccaggaagg ctggctgcac aagaggctgt ctgatctgcc     3300

tgagccacat caagtgcacc cccaagatga agaagttcat ccctggcagg tgccacacct     3360

atgaagggga caaagagtct gcccaggggg gaattggaga ggccattgtg gacatccctg     3420

agatccctgg cttcaaggac ctggaaccca tggaacagtt cattgcccag gtggacctgt     3480

gtgtggactg cactacaggc tgtctcaagg gcctggccaa tgtgcagtgc tctgacctgc     3540

tgaagaagtg gctgccccag agatgtgcca cctttgccag caagatccag ggccaggtgg     3600

acaagatcaa gggagctggg ggagattgat gagggcccaa tcaacctctg gattacaaaa     3660

tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg     3720

ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct     3780

tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg     3840

gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct     3900

gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg     3960

ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg     4020

tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc     4080

tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc     4140

gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc     4200

ggatctccct ttgggccgcc tccccgcaag cttcgcactt tttaaaagaa aagggaggac     4260

tggatgggat ttattactcc gataggacgc tggcttgtaa ctcagtctct tactaggaga     4320

ccagcttgag cctgggtgtt cgctggttag cctaacctgg ttggccacca ggggtaagga     4380

ctccttggct tagaaagcta ataaacttgc ctgcattaga gctcttacgc gtcccgggct     4440

cgagatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg     4500

cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt     4560

tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt     4620

tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca     4680

aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt     4740

gtggtttgtc caaactcatc aatgtatctt atcatgtctg tccgcttcct cgctcactga     4800

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat     4860

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca     4920

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc     4980

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata     5040

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc     5100

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc     5160

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga     5220

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     5280

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag     5340

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag     5400

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag     5460

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     5520

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga     5580

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat     5640

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga     5700

gtaaacttgg tctgacagtt agaaaaactc atcgagcatc aaatgaaact gcaatttatt     5760

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa     5820

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg     5880

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa     5940

atcaccatga gtgacgactg aatccggtga gaatggcaac agcttatgca tttctttcca     6000

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc     6060

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca     6120

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     6180

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gtttttccgg ggatcgcagt     6240

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     6300

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     6360

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     6420

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     6480

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     6540

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc     6600

ttgtgcaatg taacatcaga gattttgaga cacaacaatt ggtcgacgga tcc            6653


<210>  11
<211>  10812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM411 plasmid

<400>  11
ggtacctcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat       60

tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc      120

atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat      180

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      240

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      300

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      360

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt      420

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc      480

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      540

gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      600

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      660

caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc      720

tatataagca gagctcgctg gcttgtaact cagtctctta ctaggagacc agcttgagcc      780

tgggtgttcg ctggttagcc taacctggtt ggccaccagg ggtaaggact ccttggctta      840

gaaagctaat aaacttgcct gcattagagc ttatctgagt caagtgtcct cattgacgcc      900

tcactctctt gaacgggaat cttccttact gggttctctc tctgacccag gcgagagaaa      960

ctccagcagt ggcgcccgaa cagggacttg agtgagagtg taggcacgta cagctgagaa     1020

ggcgtcggac gcgaaggaag cgcggggtgc gacgcgacca agaaggagac ttggtgagta     1080

ggcttctcga gtgccgggaa aaagctcgag cctagttaga ggactaggag aggccgtagc     1140

cgtaactact ctgggcaagt agggcaggcg gtgggtacgc aatgggggcg gctacctcag     1200

cactaaatag gagacaatta gaccaatttg agaaaatacg acttcgcccg aacggaaaga     1260

aaaagtacca aattaaacat ttaatatggg caggcaagga gatggagcgc ttcggcctcc     1320

atgagaggtt gttggagaca gaggaggggt gtaaaagaat catagaagtc ctctaccccc     1380

tagaaccaac aggatcggag ggcttaaaaa gtctgttcaa tcttgtgtgc gtgctatatt     1440

gcttgcacaa ggaacagaaa gtgaaagaca cagaggaagc agtagcaaca gtaagacaac     1500

actgccatct agtggaaaaa gaaaaaagtg caacagagac atctagtgga caaaagaaaa     1560

atgacaaggg aatagcagcg ccacctggtg gcagtcagaa ttttccagcg caacaacaag     1620

gaaatgcctg ggtacatgta cccttgtcac cgcgcacctt aaatgcgtgg gtaaaagcag     1680

tagaggagaa aaaatttgga gcagaaatag tacccatgtt tcaagcccta tcgaattccc     1740

gtttgtgcta gggttcttag gcttcttggg ggctgctgga actgcaatgg gagcagcggc     1800

gacagccctg acggtccagt ctcagcattt gcttgctggg atactgcagc agcagaagaa     1860

tctgctggcg gctgtggagg ctcaacagca gatgttgaag ctgaccattt ggggtgttaa     1920

aaacctcaat gcccgcgtca cagcccttga gaagtaccta gaggatcagg cacgactaaa     1980

ctcctggggg tgcgcatgga aacaagtatg tcataccaca gtggagtggc cctggacaaa     2040

tcggactccg gattggcaaa atatgacttg gttggagtgg gaaagacaaa tagctgattt     2100

ggaaagcaac attacgagac aattagtgaa ggctagagaa caagaggaaa agaatctaga     2160

tgcctatcag aagttaacta gttggtcaga tttctggtct tggttcgatt tctcaaaatg     2220

gcttaacatt ttaaaaatgg gatttttagt aatagtagga ataatagggt taagattact     2280

ttacacagta tatggatgta tagtgagggt taggcaggga tatgttcctc tatctccaca     2340

gatccatatc cgcggcaatt ttaaaagaaa gggaggaata gggggacaga cttcagcaga     2400

gagactaatt aatataataa caacacaatt agaaatacaa catttacaaa ccaaaattca     2460

aaaaatttta aattttagag ccgcggagat ctcaatattg gccattagcc atattattca     2520

ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc     2580

ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat     2640

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt     2700

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc     2760

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac     2820

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata     2880

tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc     2940

agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta     3000

ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac     3060

ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc     3120

aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc     3180

gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa     3240

gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa     3300

cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggct agccaccaat     3360

gcagattgag ctgagcacct gcttcttcct gtgcctgctg aggttctgct tctctgccac     3420

caggagatac tacctggggg ctgtggagct gagctgggac tacatgcagt ctgacctggg     3480

ggagctgcct gtggatgcca ggttcccccc cagagtgccc aagagcttcc ccttcaacac     3540

ctctgtggtg tacaagaaga ccctgtttgt ggagttcact gaccacctgt tcaacattgc     3600

caagcccagg cccccctgga tgggcctgct gggccccacc atccaggctg aggtgtatga     3660

cactgtggtg atcaccctga agaacatggc cagccaccct gtgagcctgc atgctgtggg     3720

ggtgagctac tggaaggcct ctgagggggc tgagtatgat gaccagacca gccagaggga     3780

gaaggaggat gacaaggtgt tccctggggg cagccacacc tatgtgtggc aggtgctgaa     3840

ggagaatggc cccatggcct ctgaccccct gtgcctgacc tacagctacc tgagccatgt     3900

ggacctggtg aaggacctga actctggcct gattggggcc ctgctggtgt gcagggaggg     3960

cagcctggcc aaggagaaga cccagaccct gcacaagttc atcctgctgt ttgctgtgtt     4020

tgatgagggc aagagctggc actctgaaac caagaacagc ctgatgcagg acagggatgc     4080

tgcctctgcc agggcctggc ccaagatgca cactgtgaat ggctatgtga acaggagcct     4140

gcctggcctg attggctgcc acaggaagtc tgtgtactgg catgtgattg gcatgggcac     4200

cacccctgag gtgcacagca tcttcctgga gggccacacc ttcctggtca ggaaccacag     4260

gcaggccagc ctggagatca gccccatcac cttcctgact gcccagaccc tgctgatgga     4320

cctgggccag ttcctgctgt tctgccacat cagcagccac cagcatgatg gcatggaggc     4380

ctatgtgaag gtggacagct gccctgagga gccccagctg aggatgaaga acaatgagga     4440

ggctgaggac tatgatgatg acctgactga ctctgagatg gatgtggtga ggtttgatga     4500

tgacaacagc cccagcttca tccagatcag gtctgtggcc aagaagcacc ccaagacctg     4560

ggtgcactac attgctgctg aggaggagga ctgggactat gcccccctgg tgctggcccc     4620

tgatgacagg agctacaaga gccagtacct gaacaatggc ccccagagga ttggcaggaa     4680

gtacaagaag gtcaggttca tggcctacac tgatgaaacc ttcaagacca gggaggccat     4740

ccagcatgag tctggcatcc tgggccccct gctgtatggg gaggtggggg acaccctgct     4800

gatcatcttc aagaaccagg ccagcaggcc ctacaacatc tacccccatg gcatcactga     4860

tgtgaggccc ctgtacagca ggaggctgcc caagggggtg aagcacctga aggacttccc     4920

catcctgcct ggggagatct tcaagtacaa gtggactgtg actgtggagg atggccccac     4980

caagtctgac cccaggtgcc tgaccagata ctacagcagc tttgtgaaca tggagaggga     5040

cctggcctct ggcctgattg gccccctgct gatctgctac aaggagtctg tggaccagag     5100

gggcaaccag atcatgtctg acaagaggaa tgtgatcctg ttctctgtgt ttgatgagaa     5160

caggagctgg tacctgactg agaacatcca gaggttcctg cccaaccctg ctggggtgca     5220

gctggaggac cctgagttcc aggccagcaa catcatgcac agcatcaatg gctatgtgtt     5280

tgacagcctg cagctgtctg tgtgcctgca tgaggtggcc tactggtaca tcctgagcat     5340

tggggcccag actgacttcc tgtctgtgtt cttctctggc tacaccttca agcacaagat     5400

ggtgtatgag gacaccctga ccctgttccc cttctctggg gagactgtgt tcatgagcat     5460

ggagaaccct ggcctgtgga ttctgggctg ccacaactct gacttcagga acaggggcat     5520

gactgccctg ctgaaagtct ccagctgtga caagaacact ggggactact atgaggacag     5580

ctatgaggac atctctgcct acctgctgag caagaacaat gccattgagc ccaggagctt     5640

cagccagaat gccactaatg tgtctaacaa cagcaacacc agcaatgaca gcaatgtgtc     5700

tcccccagtg ctgaagaggc accagaggga gatcaccagg accaccctgc agtctgacca     5760

ggaggagatt gactatgatg acaccatctc tgtggagatg aagaaggagg actttgacat     5820

ctacgacgag gacgagaacc agagccccag gagcttccag aagaagacca ggcactactt     5880

cattgctgct gtggagaggc tgtgggacta tggcatgagc agcagccccc atgtgctgag     5940

gaacagggcc cagtctggct ctgtgcccca gttcaagaag gtggtgttcc aggagttcac     6000

tgatggcagc ttcacccagc ccctgtacag aggggagctg aatgagcacc tgggcctgct     6060

gggcccctac atcagggctg aggtggagga caacatcatg gtgaccttca ggaaccaggc     6120

cagcaggccc tacagcttct acagcagcct gatcagctat gaggaggacc agaggcaggg     6180

ggctgagccc aggaagaact ttgtgaagcc caatgaaacc aagacctact tctggaaggt     6240

gcagcaccac atggccccca ccaaggatga gtttgactgc aaggcctggg cctacttctc     6300

tgatgtggac ctggagaagg atgtgcactc tggcctgatt ggccccctgc tggtgtgcca     6360

caccaacacc ctgaaccctg cccatggcag gcaggtgact gtgcaggagt ttgccctgtt     6420

cttcaccatc tttgatgaaa ccaagagctg gtacttcact gagaacatgg agaggaactg     6480

cagggccccc tgcaacatcc agatggagga ccccaccttc aaggagaact acaggttcca     6540

tgccatcaat ggctacatca tggacaccct gcctggcctg gtgatggccc aggaccagag     6600

gatcaggtgg tacctgctga gcatgggcag caatgagaac atccacagca tccacttctc     6660

tggccatgtg ttcactgtga ggaagaagga ggagtacaag atggccctgt acaacctgta     6720

ccctggggtg tttgagactg tggagatgct gcccagcaag gctggcatct ggagggtgga     6780

gtgcctgatt ggggagcacc tgcatgctgg catgagcacc ctgttcctgg tgtacagcaa     6840

caagtgccag acccccctgg gcatggcctc tggccacatc agggacttcc agatcactgc     6900

ctctggccag tatggccagt gggcccccaa gctggccagg ctgcactact ctggcagcat     6960

caatgcctgg agcaccaagg agcccttcag ctggatcaag gtggacctgc tggcccccat     7020

gatcatccat ggcatcaaga cccagggggc caggcagaag ttcagcagcc tgtacatcag     7080

ccagttcatc atcatgtaca gcctggatgg caagaagtgg cagacctaca ggggcaacag     7140

cactggcacc ctgatggtgt tctttggcaa tgtggacagc tctggcatca agcacaacat     7200

cttcaacccc cccatcattg ccagatacat caggctgcac cccacccact acagcatcag     7260

gagcaccctg aggatggagc tgatgggctg tgacctgaac agctgcagca tgcccctggg     7320

catggagagc aaggccatct ctgatgccca gatcactgcc agcagctact tcaccaacat     7380

gtttgccacc tggagcccca gcaaggccag gctgcacctg cagggcagga gcaatgcctg     7440

gaggccccag gtcaacaacc ccaaggagtg gctgcaggtg gacttccaga agaccatgaa     7500

ggtgactggg gtgaccaccc agggggtgaa gagcctgctg accagcatgt atgtgaagga     7560

gttcctgatc agcagcagcc aggatggcca ccagtggacc ctgttcttcc agaatggcaa     7620

ggtgaaggtg ttccagggca accaggacag cttcacccct gtggtgaaca gcctggaccc     7680

ccccctgctg accagatacc tgaggattca cccccagagc tgggtgcacc agattgccct     7740

gaggatggag gtgctgggct gtgaggccca ggacctgtac tgagcggccg cgggcccaat     7800

caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct     7860

tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg     7920

gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg     7980

cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt     8040

tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt     8100

gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg     8160

ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc     8220

tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat     8280

ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc     8340

cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcaagc ttcgcacttt     8400

ttaaaagaaa agggaggact ggatgggatt tattactccg ataggacgct ggcttgtaac     8460

tcagtctctt actaggagac cagcttgagc ctgggtgttc gctggttagc ctaacctggt     8520

tggccaccag gggtaaggac tccttggctt agaaagctaa taaacttgcc tgcattagag     8580

ctcttacgcg tcccgggctc gagatccgca tctcaattag tcagcaacca tagtcccgcc     8640

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg     8700

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca     8760

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt     8820

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt     8880

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt     8940

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     9000

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     9060

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     9120

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     9180

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     9240

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     9300

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     9360

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     9420

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     9480

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     9540

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct     9600

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     9660

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     9720

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     9780

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     9840

caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca tcgagcatca     9900

aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt     9960

tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc    10020

ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa    10080

taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaca    10140

gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat    10200

cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc    10260

gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg    10320

ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg    10380

tttttccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct    10440

tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa    10500

catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc    10560

catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc    10620

catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt    10680

gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc    10740

atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacaattg    10800

gtcgacggat cc                                                        10812


<210>  12
<211>  10519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM413 plasmid

<400>  12
ggtacctcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat       60

tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc      120

atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat      180

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      240

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      300

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      360

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt      420

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc      480

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      540

gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      600

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      660

caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc      720

tatataagca gagctcgctg gcttgtaact cagtctctta ctaggagacc agcttgagcc      780

tgggtgttcg ctggttagcc taacctggtt ggccaccagg ggtaaggact ccttggctta      840

gaaagctaat aaacttgcct gcattagagc ttatctgagt caagtgtcct cattgacgcc      900

tcactctctt gaacgggaat cttccttact gggttctctc tctgacccag gcgagagaaa      960

ctccagcagt ggcgcccgaa cagggacttg agtgagagtg taggcacgta cagctgagaa     1020

ggcgtcggac gcgaaggaag cgcggggtgc gacgcgacca agaaggagac ttggtgagta     1080

ggcttctcga gtgccgggaa aaagctcgag cctagttaga ggactaggag aggccgtagc     1140

cgtaactact ctgggcaagt agggcaggcg gtgggtacgc aatgggggcg gctacctcag     1200

cactaaatag gagacaatta gaccaatttg agaaaatacg acttcgcccg aacggaaaga     1260

aaaagtacca aattaaacat ttaatatggg caggcaagga gatggagcgc ttcggcctcc     1320

atgagaggtt gttggagaca gaggaggggt gtaaaagaat catagaagtc ctctaccccc     1380

tagaaccaac aggatcggag ggcttaaaaa gtctgttcaa tcttgtgtgc gtgctatatt     1440

gcttgcacaa ggaacagaaa gtgaaagaca cagaggaagc agtagcaaca gtaagacaac     1500

actgccatct agtggaaaaa gaaaaaagtg caacagagac atctagtgga caaaagaaaa     1560

atgacaaggg aatagcagcg ccacctggtg gcagtcagaa ttttccagcg caacaacaag     1620

gaaatgcctg ggtacatgta cccttgtcac cgcgcacctt aaatgcgtgg gtaaaagcag     1680

tagaggagaa aaaatttgga gcagaaatag tacccatgtt tcaagcccta tcgaattccc     1740

gtttgtgcta gggttcttag gcttcttggg ggctgctgga actgcaatgg gagcagcggc     1800

gacagccctg acggtccagt ctcagcattt gcttgctggg atactgcagc agcagaagaa     1860

tctgctggcg gctgtggagg ctcaacagca gatgttgaag ctgaccattt ggggtgttaa     1920

aaacctcaat gcccgcgtca cagcccttga gaagtaccta gaggatcagg cacgactaaa     1980

ctcctggggg tgcgcatgga aacaagtatg tcataccaca gtggagtggc cctggacaaa     2040

tcggactccg gattggcaaa atatgacttg gttggagtgg gaaagacaaa tagctgattt     2100

ggaaagcaac attacgagac aattagtgaa ggctagagaa caagaggaaa agaatctaga     2160

tgcctatcag aagttaacta gttggtcaga tttctggtct tggttcgatt tctcaaaatg     2220

gcttaacatt ttaaaaatgg gatttttagt aatagtagga ataatagggt taagattact     2280

ttacacagta tatggatgta tagtgagggt taggcaggga tatgttcctc tatctccaca     2340

gatccatatc cgcggcaatt ttaaaagaaa gggaggaata gggggacaga cttcagcaga     2400

gagactaatt aatataataa caacacaatt agaaatacaa catttacaaa ccaaaattca     2460

aaaaatttta aattttagag ccgcggagat ctgttacata acttatggta aatggcctgc     2520

ctggctgact gcccaatgac ccctgcccaa tgatgtcaat aatgatgtat gttcccatgt     2580

aatgccaata gggactttcc attgatgtca atgggtggag tatttatggt aactgcccac     2640

ttggcagtac atcaagtgta tcatatgcca agtatgcccc ctattgatgt caatgatggt     2700

aaatggcctg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag     2760

tacatctatg tattagtcat tgctattacc atgggaattc actagtggag aagagcatgc     2820

ttgagggctg agtgcccctc agtgggcaga gagcacatgg cccacagtcc ctgagaagtt     2880

ggggggaggg gtgggcaatt gaactggtgc ctagagaagg tggggcttgg gtaaactggg     2940

aaagtgatgt ggtgtactgg ctccaccttt ttccccaggg tgggggagaa ccatatataa     3000

gtgcagtagt ctctgtgaac attcaagctt ctgccttctc cctcctgtga gtttgctagc     3060

caccaatgca gattgagctg agcacctgct tcttcctgtg cctgctgagg ttctgcttct     3120

ctgccaccag gagatactac ctgggggctg tggagctgag ctgggactac atgcagtctg     3180

acctggggga gctgcctgtg gatgccaggt tcccccccag agtgcccaag agcttcccct     3240

tcaacacctc tgtggtgtac aagaagaccc tgtttgtgga gttcactgac cacctgttca     3300

acattgccaa gcccaggccc ccctggatgg gcctgctggg ccccaccatc caggctgagg     3360

tgtatgacac tgtggtgatc accctgaaga acatggccag ccaccctgtg agcctgcatg     3420

ctgtgggggt gagctactgg aaggcctctg agggggctga gtatgatgac cagaccagcc     3480

agagggagaa ggaggatgac aaggtgttcc ctgggggcag ccacacctat gtgtggcagg     3540

tgctgaagga gaatggcccc atggcctctg accccctgtg cctgacctac agctacctga     3600

gccatgtgga cctggtgaag gacctgaact ctggcctgat tggggccctg ctggtgtgca     3660

gggagggcag cctggccaag gagaagaccc agaccctgca caagttcatc ctgctgtttg     3720

ctgtgtttga tgagggcaag agctggcact ctgaaaccaa gaacagcctg atgcaggaca     3780

gggatgctgc ctctgccagg gcctggccca agatgcacac tgtgaatggc tatgtgaaca     3840

ggagcctgcc tggcctgatt ggctgccaca ggaagtctgt gtactggcat gtgattggca     3900

tgggcaccac ccctgaggtg cacagcatct tcctggaggg ccacaccttc ctggtcagga     3960

accacaggca ggccagcctg gagatcagcc ccatcacctt cctgactgcc cagaccctgc     4020

tgatggacct gggccagttc ctgctgttct gccacatcag cagccaccag catgatggca     4080

tggaggccta tgtgaaggtg gacagctgcc ctgaggagcc ccagctgagg atgaagaaca     4140

atgaggaggc tgaggactat gatgatgacc tgactgactc tgagatggat gtggtgaggt     4200

ttgatgatga caacagcccc agcttcatcc agatcaggtc tgtggccaag aagcacccca     4260

agacctgggt gcactacatt gctgctgagg aggaggactg ggactatgcc cccctggtgc     4320

tggcccctga tgacaggagc tacaagagcc agtacctgaa caatggcccc cagaggattg     4380

gcaggaagta caagaaggtc aggttcatgg cctacactga tgaaaccttc aagaccaggg     4440

aggccatcca gcatgagtct ggcatcctgg gccccctgct gtatggggag gtgggggaca     4500

ccctgctgat catcttcaag aaccaggcca gcaggcccta caacatctac ccccatggca     4560

tcactgatgt gaggcccctg tacagcagga ggctgcccaa gggggtgaag cacctgaagg     4620

acttccccat cctgcctggg gagatcttca agtacaagtg gactgtgact gtggaggatg     4680

gccccaccaa gtctgacccc aggtgcctga ccagatacta cagcagcttt gtgaacatgg     4740

agagggacct ggcctctggc ctgattggcc ccctgctgat ctgctacaag gagtctgtgg     4800

accagagggg caaccagatc atgtctgaca agaggaatgt gatcctgttc tctgtgtttg     4860

atgagaacag gagctggtac ctgactgaga acatccagag gttcctgccc aaccctgctg     4920

gggtgcagct ggaggaccct gagttccagg ccagcaacat catgcacagc atcaatggct     4980

atgtgtttga cagcctgcag ctgtctgtgt gcctgcatga ggtggcctac tggtacatcc     5040

tgagcattgg ggcccagact gacttcctgt ctgtgttctt ctctggctac accttcaagc     5100

acaagatggt gtatgaggac accctgaccc tgttcccctt ctctggggag actgtgttca     5160

tgagcatgga gaaccctggc ctgtggattc tgggctgcca caactctgac ttcaggaaca     5220

ggggcatgac tgccctgctg aaagtctcca gctgtgacaa gaacactggg gactactatg     5280

aggacagcta tgaggacatc tctgcctacc tgctgagcaa gaacaatgcc attgagccca     5340

ggagcttcag ccagaatgcc actaatgtgt ctaacaacag caacaccagc aatgacagca     5400

atgtgtctcc cccagtgctg aagaggcacc agagggagat caccaggacc accctgcagt     5460

ctgaccagga ggagattgac tatgatgaca ccatctctgt ggagatgaag aaggaggact     5520

ttgacatcta cgacgaggac gagaaccaga gccccaggag cttccagaag aagaccaggc     5580

actacttcat tgctgctgtg gagaggctgt gggactatgg catgagcagc agcccccatg     5640

tgctgaggaa cagggcccag tctggctctg tgccccagtt caagaaggtg gtgttccagg     5700

agttcactga tggcagcttc acccagcccc tgtacagagg ggagctgaat gagcacctgg     5760

gcctgctggg cccctacatc agggctgagg tggaggacaa catcatggtg accttcagga     5820

accaggccag caggccctac agcttctaca gcagcctgat cagctatgag gaggaccaga     5880

ggcagggggc tgagcccagg aagaactttg tgaagcccaa tgaaaccaag acctacttct     5940

ggaaggtgca gcaccacatg gcccccacca aggatgagtt tgactgcaag gcctgggcct     6000

acttctctga tgtggacctg gagaaggatg tgcactctgg cctgattggc cccctgctgg     6060

tgtgccacac caacaccctg aaccctgccc atggcaggca ggtgactgtg caggagtttg     6120

ccctgttctt caccatcttt gatgaaacca agagctggta cttcactgag aacatggaga     6180

ggaactgcag ggccccctgc aacatccaga tggaggaccc caccttcaag gagaactaca     6240

ggttccatgc catcaatggc tacatcatgg acaccctgcc tggcctggtg atggcccagg     6300

accagaggat caggtggtac ctgctgagca tgggcagcaa tgagaacatc cacagcatcc     6360

acttctctgg ccatgtgttc actgtgagga agaaggagga gtacaagatg gccctgtaca     6420

acctgtaccc tggggtgttt gagactgtgg agatgctgcc cagcaaggct ggcatctgga     6480

gggtggagtg cctgattggg gagcacctgc atgctggcat gagcaccctg ttcctggtgt     6540

acagcaacaa gtgccagacc cccctgggca tggcctctgg ccacatcagg gacttccaga     6600

tcactgcctc tggccagtat ggccagtggg cccccaagct ggccaggctg cactactctg     6660

gcagcatcaa tgcctggagc accaaggagc ccttcagctg gatcaaggtg gacctgctgg     6720

cccccatgat catccatggc atcaagaccc agggggccag gcagaagttc agcagcctgt     6780

acatcagcca gttcatcatc atgtacagcc tggatggcaa gaagtggcag acctacaggg     6840

gcaacagcac tggcaccctg atggtgttct ttggcaatgt ggacagctct ggcatcaagc     6900

acaacatctt caaccccccc atcattgcca gatacatcag gctgcacccc acccactaca     6960

gcatcaggag caccctgagg atggagctga tgggctgtga cctgaacagc tgcagcatgc     7020

ccctgggcat ggagagcaag gccatctctg atgcccagat cactgccagc agctacttca     7080

ccaacatgtt tgccacctgg agccccagca aggccaggct gcacctgcag ggcaggagca     7140

atgcctggag gccccaggtc aacaacccca aggagtggct gcaggtggac ttccagaaga     7200

ccatgaaggt gactggggtg accacccagg gggtgaagag cctgctgacc agcatgtatg     7260

tgaaggagtt cctgatcagc agcagccagg atggccacca gtggaccctg ttcttccaga     7320

atggcaaggt gaaggtgttc cagggcaacc aggacagctt cacccctgtg gtgaacagcc     7380

tggacccccc cctgctgacc agatacctga ggattcaccc ccagagctgg gtgcaccaga     7440

ttgccctgag gatggaggtg ctgggctgtg aggcccagga cctgtactga gcggccgcgg     7500

gcccaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt     7560

tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc     7620

ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga     7680

gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc     7740

cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct     7800

ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg     7860

gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct     7920

gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc     7980

cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg     8040

tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcaagcttc     8100

gcacttttta aaagaaaagg gaggactgga tgggatttat tactccgata ggacgctggc     8160

ttgtaactca gtctcttact aggagaccag cttgagcctg ggtgttcgct ggttagccta     8220

acctggttgg ccaccagggg taaggactcc ttggcttaga aagctaataa acttgcctgc     8280

attagagctc ttacgcgtcc cgggctcgag atccgcatct caattagtca gcaaccatag     8340

tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc     8400

cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc     8460

tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt     8520

gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa     8580

agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca     8640

tgtctgtccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     8700

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     8760

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     8820

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     8880

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     8940

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     9000

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     9060

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     9120

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     9180

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     9240

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     9300

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     9360

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     9420

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     9480

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     9540

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa aaactcatcg     9600

agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa     9660

agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc     9720

tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg     9780

tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat     9840

ggcaacagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca     9900

tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga     9960

aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg    10020

aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg    10080

aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata    10140

aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca    10200

tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg    10260

ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat    10320

ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt    10380

tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt    10440

attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca    10500

acaattggtc gacggatcc                                                 10519


<210>  13
<211>  11400
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM412 plasmid

<400>  13
ggtacctcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat       60

tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc      120

atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat      180

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      240

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      300

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      360

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt      420

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc      480

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      540

gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      600

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      660

caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc      720

tatataagca gagctcgctg gcttgtaact cagtctctta ctaggagacc agcttgagcc      780

tgggtgttcg ctggttagcc taacctggtt ggccaccagg ggtaaggact ccttggctta      840

gaaagctaat aaacttgcct gcattagagc ttatctgagt caagtgtcct cattgacgcc      900

tcactctctt gaacgggaat cttccttact gggttctctc tctgacccag gcgagagaaa      960

ctccagcagt ggcgcccgaa cagggacttg agtgagagtg taggcacgta cagctgagaa     1020

ggcgtcggac gcgaaggaag cgcggggtgc gacgcgacca agaaggagac ttggtgagta     1080

ggcttctcga gtgccgggaa aaagctcgag cctagttaga ggactaggag aggccgtagc     1140

cgtaactact ctgggcaagt agggcaggcg gtgggtacgc aatgggggcg gctacctcag     1200

cactaaatag gagacaatta gaccaatttg agaaaatacg acttcgcccg aacggaaaga     1260

aaaagtacca aattaaacat ttaatatggg caggcaagga gatggagcgc ttcggcctcc     1320

atgagaggtt gttggagaca gaggaggggt gtaaaagaat catagaagtc ctctaccccc     1380

tagaaccaac aggatcggag ggcttaaaaa gtctgttcaa tcttgtgtgc gtgctatatt     1440

gcttgcacaa ggaacagaaa gtgaaagaca cagaggaagc agtagcaaca gtaagacaac     1500

actgccatct agtggaaaaa gaaaaaagtg caacagagac atctagtgga caaaagaaaa     1560

atgacaaggg aatagcagcg ccacctggtg gcagtcagaa ttttccagcg caacaacaag     1620

gaaatgcctg ggtacatgta cccttgtcac cgcgcacctt aaatgcgtgg gtaaaagcag     1680

tagaggagaa aaaatttgga gcagaaatag tacccatgtt tcaagcccta tcgaattccc     1740

gtttgtgcta gggttcttag gcttcttggg ggctgctgga actgcaatgg gagcagcggc     1800

gacagccctg acggtccagt ctcagcattt gcttgctggg atactgcagc agcagaagaa     1860

tctgctggcg gctgtggagg ctcaacagca gatgttgaag ctgaccattt ggggtgttaa     1920

aaacctcaat gcccgcgtca cagcccttga gaagtaccta gaggatcagg cacgactaaa     1980

ctcctggggg tgcgcatgga aacaagtatg tcataccaca gtggagtggc cctggacaaa     2040

tcggactccg gattggcaaa atatgacttg gttggagtgg gaaagacaaa tagctgattt     2100

ggaaagcaac attacgagac aattagtgaa ggctagagaa caagaggaaa agaatctaga     2160

tgcctatcag aagttaacta gttggtcaga tttctggtct tggttcgatt tctcaaaatg     2220

gcttaacatt ttaaaaatgg gatttttagt aatagtagga ataatagggt taagattact     2280

ttacacagta tatggatgta tagtgagggt taggcaggga tatgttcctc tatctccaca     2340

gatccatatc cgcggcaatt ttaaaagaaa gggaggaata gggggacaga cttcagcaga     2400

gagactaatt aatataataa caacacaatt agaaatacaa catttacaaa ccaaaattca     2460

aaaaatttta aattttagag ccgcggagat ctcaatattg gccattagcc atattattca     2520

ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc     2580

ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat     2640

tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt     2700

tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc     2760

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac     2820

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata     2880

tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc     2940

agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta     3000

ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac     3060

ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc     3120

aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc     3180

gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa     3240

gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa     3300

cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggct agccaccaat     3360

gcagattgag ctgagcacct gcttcttcct gtgcctgctg aggttctgct tctctgccac     3420

caggagatac tacctggggg ctgtggagct gagctgggac tacatgcagt ctgacctggg     3480

ggagctgcct gtggatgcca ggttcccccc cagagtgccc aagagcttcc ccttcaacac     3540

ctctgtggtg tacaagaaga ccctgtttgt ggagttcact gaccacctgt tcaacattgc     3600

caagcccagg cccccctgga tgggcctgct gggccccacc atccaggctg aggtgtatga     3660

cactgtggtg atcaccctga agaacatggc cagccaccct gtgagcctgc atgctgtggg     3720

ggtgagctac tggaaggcct ctgagggggc tgagtatgat gaccagacca gccagaggga     3780

gaaggaggat gacaaggtgt tccctggggg cagccacacc tatgtgtggc aggtgctgaa     3840

ggagaatggc cccatggcct ctgaccccct gtgcctgacc tacagctacc tgagccatgt     3900

ggacctggtg aaggacctga actctggcct gattggggcc ctgctggtgt gcagggaggg     3960

cagcctggcc aaggagaaga cccagaccct gcacaagttc atcctgctgt ttgctgtgtt     4020

tgatgagggc aagagctggc actctgaaac caagaacagc ctgatgcagg acagggatgc     4080

tgcctctgcc agggcctggc ccaagatgca cactgtgaat ggctatgtga acaggagcct     4140

gcctggcctg attggctgcc acaggaagtc tgtgtactgg catgtgattg gcatgggcac     4200

cacccctgag gtgcacagca tcttcctgga gggccacacc ttcctggtca ggaaccacag     4260

gcaggccagc ctggagatca gccccatcac cttcctgact gcccagaccc tgctgatgga     4320

cctgggccag ttcctgctgt tctgccacat cagcagccac cagcatgatg gcatggaggc     4380

ctatgtgaag gtggacagct gccctgagga gccccagctg aggatgaaga acaatgagga     4440

ggctgaggac tatgatgatg acctgactga ctctgagatg gatgtggtga ggtttgatga     4500

tgacaacagc cccagcttca tccagatcag gtctgtggcc aagaagcacc ccaagacctg     4560

ggtgcactac attgctgctg aggaggagga ctgggactat gcccccctgg tgctggcccc     4620

tgatgacagg agctacaaga gccagtacct gaacaatggc ccccagagga ttggcaggaa     4680

gtacaagaag gtcaggttca tggcctacac tgatgaaacc ttcaagacca gggaggccat     4740

ccagcatgag tctggcatcc tgggccccct gctgtatggg gaggtggggg acaccctgct     4800

gatcatcttc aagaaccagg ccagcaggcc ctacaacatc tacccccatg gcatcactga     4860

tgtgaggccc ctgtacagca ggaggctgcc caagggggtg aagcacctga aggacttccc     4920

catcctgcct ggggagatct tcaagtacaa gtggactgtg actgtggagg atggccccac     4980

caagtctgac cccaggtgcc tgaccagata ctacagcagc tttgtgaaca tggagaggga     5040

cctggcctct ggcctgattg gccccctgct gatctgctac aaggagtctg tggaccagag     5100

gggcaaccag atcatgtctg acaagaggaa tgtgatcctg ttctctgtgt ttgatgagaa     5160

caggagctgg tacctgactg agaacatcca gaggttcctg cccaaccctg ctggggtgca     5220

gctggaggac cctgagttcc aggccagcaa catcatgcac agcatcaatg gctatgtgtt     5280

tgacagcctg cagctgtctg tgtgcctgca tgaggtggcc tactggtaca tcctgagcat     5340

tggggcccag actgacttcc tgtctgtgtt cttctctggc tacaccttca agcacaagat     5400

ggtgtatgag gacaccctga ccctgttccc cttctctggg gagactgtgt tcatgagcat     5460

ggagaaccct ggcctgtgga ttctgggctg ccacaactct gacttcagga acaggggcat     5520

gactgccctg ctgaaagtct ccagctgtga caagaacact ggggactact atgaggacag     5580

ctatgaggac atctctgcct acctgctgag caagaacaat gccattgagc ccaggagctt     5640

cagccagaac agcaggcacc ccagcaccag gcagaagcag ttcaatgcca ccaccatccc     5700

tgagaatgac atagagaaga cagacccatg gtttgcccac cggaccccca tgcccaagat     5760

ccagaatgtg agcagctctg acctgctgat gctgctgagg cagagcccca ccccccatgg     5820

cctgagcctg tctgacctgc aggaggccaa gtatgaaacc ttctctgatg accccagccc     5880

tggggccatt gacagcaaca acagcctgtc tgagatgacc cacttcaggc cccagctgca     5940

ccactctggg gacatggtgt tcacccctga gtctggcctg cagctgaggc tgaatgagaa     6000

gctgggcacc actgctgcca ctgagctgaa gaagctggac ttcaaagtct ccagcaccag     6060

caacaacctg atcagcacca tcccctctga caacctggct gctggcactg acaacaccag     6120

cagcctgggc ccccccagca tgcctgtgca ctatgacagc cagctggaca ccaccctgtt     6180

tggcaagaag agcagccccc tgactgagtc tgggggcccc ctgagcctgt ctgaggagaa     6240

caatgacagc aagctgctgg agtctggcct gatgaacagc caggagagca gctggggcaa     6300

gaatgtgagc agcagggaga tcaccaggac caccctgcag tctgaccagg aggagattga     6360

ctatgatgac accatctctg tggagatgaa gaaggaggac tttgacatct acgacgagga     6420

cgagaaccag agccccagga gcttccagaa gaagaccagg cactacttca ttgctgctgt     6480

ggagaggctg tgggactatg gcatgagcag cagcccccat gtgctgagga acagggccca     6540

gtctggctct gtgccccagt tcaagaaggt ggtgttccag gagttcactg atggcagctt     6600

cacccagccc ctgtacagag gggagctgaa tgagcacctg ggcctgctgg gcccctacat     6660

cagggctgag gtggaggaca acatcatggt gaccttcagg aaccaggcca gcaggcccta     6720

cagcttctac agcagcctga tcagctatga ggaggaccag aggcaggggg ctgagcccag     6780

gaagaacttt gtgaagccca atgaaaccaa gacctacttc tggaaggtgc agcaccacat     6840

ggcccccacc aaggatgagt ttgactgcaa ggcctgggcc tacttctctg atgtggacct     6900

ggagaaggat gtgcactctg gcctgattgg ccccctgctg gtgtgccaca ccaacaccct     6960

gaaccctgcc catggcaggc aggtgactgt gcaggagttt gccctgttct tcaccatctt     7020

tgatgaaacc aagagctggt acttcactga gaacatggag aggaactgca gggccccctg     7080

caacatccag atggaggacc ccaccttcaa ggagaactac aggttccatg ccatcaatgg     7140

ctacatcatg gacaccctgc ctggcctggt gatggcccag gaccagagga tcaggtggta     7200

cctgctgagc atgggcagca atgagaacat ccacagcatc cacttctctg gccatgtgtt     7260

cactgtgagg aagaaggagg agtacaagat ggccctgtac aacctgtacc ctggggtgtt     7320

tgagactgtg gagatgctgc ccagcaaggc tggcatctgg agggtggagt gcctgattgg     7380

ggagcacctg catgctggca tgagcaccct gttcctggtg tacagcaaca agtgccagac     7440

ccccctgggc atggcctctg gccacatcag ggacttccag atcactgcct ctggccagta     7500

tggccagtgg gcccccaagc tggccaggct gcactactct ggcagcatca atgcctggag     7560

caccaaggag cccttcagct ggatcaaggt ggacctgctg gcccccatga tcatccatgg     7620

catcaagacc cagggggcca ggcagaagtt cagcagcctg tacatcagcc agttcatcat     7680

catgtacagc ctggatggca agaagtggca gacctacagg ggcaacagca ctggcaccct     7740

gatggtgttc tttggcaatg tggacagctc tggcatcaag cacaacatct tcaacccccc     7800

catcattgcc agatacatca ggctgcaccc cacccactac agcatcagga gcaccctgag     7860

gatggagctg atgggctgtg acctgaacag ctgcagcatg cccctgggca tggagagcaa     7920

ggccatctct gatgcccaga tcactgccag cagctacttc accaacatgt ttgccacctg     7980

gagccccagc aaggccaggc tgcacctgca gggcaggagc aatgcctgga ggccccaggt     8040

caacaacccc aaggagtggc tgcaggtgga cttccagaag accatgaagg tgactggggt     8100

gaccacccag ggggtgaaga gcctgctgac cagcatgtat gtgaaggagt tcctgatcag     8160

cagcagccag gatggccacc agtggaccct gttcttccag aatggcaagg tgaaggtgtt     8220

ccagggcaac caggacagct tcacccctgt ggtgaacagc ctggaccccc ccctgctgac     8280

cagatacctg aggattcacc cccagagctg ggtgcaccag attgccctga ggatggaggt     8340

gctgggctgt gaggcccagg acctgtactg agcggccgcg ggcccaatca acctctggat     8400

tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt     8460

ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc     8520

tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg     8580

caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc     8640

accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa     8700

ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat     8760

tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc     8820

tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt     8880

ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag     8940

acgagtcgga tctccctttg ggccgcctcc ccgcaagctt cgcacttttt aaaagaaaag     9000

ggaggactgg atgggattta ttactccgat aggacgctgg cttgtaactc agtctcttac     9060

taggagacca gcttgagcct gggtgttcgc tggttagcct aacctggttg gccaccaggg     9120

gtaaggactc cttggcttag aaagctaata aacttgcctg cattagagct cttacgcgtc     9180

ccgggctcga gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc     9240

catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt     9300

ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg     9360

aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat     9420

ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat     9480

tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtcc gcttcctcgc     9540

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     9600

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     9660

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     9720

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     9780

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     9840

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     9900

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     9960

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt    10020

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca    10080

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca    10140

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag    10200

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca    10260

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg    10320

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa    10380

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta    10440

tatatgagta aacttggtct gacagttaga aaaactcatc gagcatcaaa tgaaactgca    10500

atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag    10560

gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc    10620

cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa    10680

gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaacagc ttatgcattt    10740

ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa    10800

ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa    10860

aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa    10920

caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt tttccgggga    10980

tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa    11040

gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa    11100

cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat    11160

agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag    11220

catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca    11280

taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat    11340

ttttatcttg tgcaatgtaa catcagagat tttgagacac aacaattggt cgacggatcc    11400


<210>  14
<211>  11108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGM414 plasmid

<400>  14
ggtacctcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat       60

tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc      120

atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat      180

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      240

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      300

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      360

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt      420

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc      480

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      540

gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      600

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      660

caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc      720

tatataagca gagctcgctg gcttgtaact cagtctctta ctaggagacc agcttgagcc      780

tgggtgttcg ctggttagcc taacctggtt ggccaccagg ggtaaggact ccttggctta      840

gaaagctaat aaacttgcct gcattagagc ttatctgagt caagtgtcct cattgacgcc      900

tcactctctt gaacgggaat cttccttact gggttctctc tctgacccag gcgagagaaa      960

ctccagcagt ggcgcccgaa cagggacttg agtgagagtg taggcacgta cagctgagaa     1020

ggcgtcggac gcgaaggaag cgcggggtgc gacgcgacca agaaggagac ttggtgagta     1080

ggcttctcga gtgccgggaa aaagctcgag cctagttaga ggactaggag aggccgtagc     1140

cgtaactact cttgggcaag tagggcaggc ggtgggtacg caatgggggc ggctacctca     1200

gcactaaata ggagacaatt agaccaattt gagaaaatac gacttcgccc gaacggaaag     1260

aaaaagtacc aaattaaaca tttaatatgg gcaggcaagg agatggagcg cttcggcctc     1320

catgagaggt tgttggagac agaggagggg tgtaaaagaa tcatagaagt cctctacccc     1380

ctagaaccaa caggatcgga gggcttaaaa agtctgttca atcttgtgtg cgtgctatat     1440

tgcttgcaca aggaacagaa agtgaaagac acagaggaag cagtagcaac agtaagacaa     1500

cactgccatc tagtggaaaa agaaaaaagt gcaacagaga catctagtgg acaaaagaaa     1560

aatgacaagg gaatagcagc gccacctggt ggcagtcaga attttccagc gcaacaacaa     1620

ggaaatgcct gggtacatgt acccttgtca ccgcgcacct taaatgcgtg ggtaaaagca     1680

gtagaggaga aaaaatttgg agcagaaata gtacccatgt ttcaagccct atcgaattcc     1740

cgtttgtgct agggttctta ggcttcttgg gggctgctgg aactgcaatg ggagcagcgg     1800

cgacagccct gacggtccag tctcagcatt tgcttgctgg gatactgcag cagcagaaga     1860

atctgctggc ggctgtggag gctcaacagc agatgttgaa gctgaccatt tggggtgtta     1920

aaaacctcaa tgcccgcgtc acagcccttg agaagtacct agaggatcag gcacgactaa     1980

actcctgggg gtgcgcatgg aaacaagtat gtcataccac agtggagtgg ccctggacaa     2040

atcggactcc ggattggcaa aatatgactt ggttggagtg ggaaagacaa atagctgatt     2100

tggaaagcaa cattacgaga caattagtga aggctagaga acaagaggaa aagaatctag     2160

atgcctatca gaagttaact agttggtcag atttctggtc ttggttcgat ttctcaaaat     2220

ggcttaacat tttaaaaatg ggatttttag taatagtagg aataataggg ttaagattac     2280

tttacacagt atatggatgt atagtgaggg ttaggcaggg atatgttcct ctatctccac     2340

agatccatat ccgcggcaat tttaaaagaa agggaggaat agggggacag acttcagcag     2400

agagactaat taatataata acaacacaat tagaaataca acatttacaa accaaaattc     2460

aaaaaatttt aaattttaga gccgcggaga tctgttacat aacttatggt aaatggcctg     2520

cctggctgac tgcccaatga cccctgccca atgatgtcaa taatgatgta tgttcccatg     2580

taatgccaat agggactttc cattgatgtc aatgggtgga gtatttatgg taactgccca     2640

cttggcagta catcaagtgt atcatatgcc aagtatgccc cctattgatg tcaatgatgg     2700

taaatggcct gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca     2760

gtacatctat gtattagtca ttgctattac catgggaatt cactagtgga gaagagcatg     2820

cttgagggct gagtgcccct cagtgggcag agagcacatg gcccacagtc cctgagaagt     2880

tggggggagg ggtgggcaat tgaactggtg cctagagaag gtggggcttg ggtaaactgg     2940

gaaagtgatg tggtgtactg gctccacctt tttccccagg gtgggggaga accatatata     3000

agtgcagtag tctctgtgaa cattcaagct tctgccttct ccctcctgtg agtttgctag     3060

ccaccaatgc agattgagct gagcacctgc ttcttcctgt gcctgctgag gttctgcttc     3120

tctgccacca ggagatacta cctgggggct gtggagctga gctgggacta catgcagtct     3180

gacctggggg agctgcctgt ggatgccagg ttccccccca gagtgcccaa gagcttcccc     3240

ttcaacacct ctgtggtgta caagaagacc ctgtttgtgg agttcactga ccacctgttc     3300

aacattgcca agcccaggcc cccctggatg ggcctgctgg gccccaccat ccaggctgag     3360

gtgtatgaca ctgtggtgat caccctgaag aacatggcca gccaccctgt gagcctgcat     3420

gctgtggggg tgagctactg gaaggcctct gagggggctg agtatgatga ccagaccagc     3480

cagagggaga aggaggatga caaggtgttc cctgggggca gccacaccta tgtgtggcag     3540

gtgctgaagg agaatggccc catggcctct gaccccctgt gcctgaccta cagctacctg     3600

agccatgtgg acctggtgaa ggacctgaac tctggcctga ttggggccct gctggtgtgc     3660

agggagggca gcctggccaa ggagaagacc cagaccctgc acaagttcat cctgctgttt     3720

gctgtgtttg atgagggcaa gagctggcac tctgaaacca agaacagcct gatgcaggac     3780

agggatgctg cctctgccag ggcctggccc aagatgcaca ctgtgaatgg ctatgtgaac     3840

aggagcctgc ctggcctgat tggctgccac aggaagtctg tgtactggca tgtgattggc     3900

atgggcacca cccctgaggt gcacagcatc ttcctggagg gccacacctt cctggtcagg     3960

aaccacaggc aggccagcct ggagatcagc cccatcacct tcctgactgc ccagaccctg     4020

ctgatggacc tgggccagtt cctgctgttc tgccacatca gcagccacca gcatgatggc     4080

atggaggcct atgtgaaggt ggacagctgc cctgaggagc cccagctgag gatgaagaac     4140

aatgaggagg ctgaggacta tgatgatgac ctgactgact ctgagatgga tgtggtgagg     4200

tttgatgatg acaacagccc cagcttcatc cagatcaggt ctgtggccaa gaagcacccc     4260

aagacctggg tgcactacat tgctgctgag gaggaggact gggactatgc ccccctggtg     4320

ctggcccctg atgacaggag ctacaagagc cagtacctga acaatggccc ccagaggatt     4380

ggcaggaagt acaagaaggt caggttcatg gcctacactg atgaaacctt caagaccagg     4440

gaggccatcc agcatgagtc tggcatcctg ggccccctgc tgtatgggga ggtgggggac     4500

accctgctga tcatcttcaa gaaccaggcc agcaggccct acaacatcta cccccatggc     4560

atcactgatg tgaggcccct gtacagcagg aggctgccca agggggtgaa gcacctgaag     4620

gacttcccca tcctgcctgg ggagatcttc aagtacaagt ggactgtgac tgtggaggat     4680

ggccccacca agtctgaccc caggtgcctg accagatact acagcagctt tgtgaacatg     4740

gagagggacc tggcctctgg cctgattggc cccctgctga tctgctacaa ggagtctgtg     4800

gaccagaggg gcaaccagat catgtctgac aagaggaatg tgatcctgtt ctctgtgttt     4860

gatgagaaca ggagctggta cctgactgag aacatccaga ggttcctgcc caaccctgct     4920

ggggtgcagc tggaggaccc tgagttccag gccagcaaca tcatgcacag catcaatggc     4980

tatgtgtttg acagcctgca gctgtctgtg tgcctgcatg aggtggccta ctggtacatc     5040

ctgagcattg gggcccagac tgacttcctg tctgtgttct tctctggcta caccttcaag     5100

cacaagatgg tgtatgagga caccctgacc ctgttcccct tctctgggga gactgtgttc     5160

atgagcatgg agaaccctgg cctgtggatt ctgggctgcc acaactctga cttcaggaac     5220

aggggcatga ctgccctgct gaaagtctcc agctgtgaca agaacactgg ggactactat     5280

gaggacagct atgaggacat ctctgcctac ctgctgagca agaacaatgc cattgagccc     5340

aggagcttca gccagaacag caggcacccc agcaccaggc agaagcagtt caatgccacc     5400

accatccctg agaatgacat agagaagaca gacccatggt ttgcccaccg gacccccatg     5460

cccaagatcc agaatgtgag cagctctgac ctgctgatgc tgctgaggca gagccccacc     5520

ccccatggcc tgagcctgtc tgacctgcag gaggccaagt atgaaacctt ctctgatgac     5580

cccagccctg gggccattga cagcaacaac agcctgtctg agatgaccca cttcaggccc     5640

cagctgcacc actctgggga catggtgttc acccctgagt ctggcctgca gctgaggctg     5700

aatgagaagc tgggcaccac tgctgccact gagctgaaga agctggactt caaagtctcc     5760

agcaccagca acaacctgat cagcaccatc ccctctgaca acctggctgc tggcactgac     5820

aacaccagca gcctgggccc ccccagcatg cctgtgcact atgacagcca gctggacacc     5880

accctgtttg gcaagaagag cagccccctg actgagtctg ggggccccct gagcctgtct     5940

gaggagaaca atgacagcaa gctgctggag tctggcctga tgaacagcca ggagagcagc     6000

tggggcaaga atgtgagcag cagggagatc accaggacca ccctgcagtc tgaccaggag     6060

gagattgact atgatgacac catctctgtg gagatgaaga aggaggactt tgacatctac     6120

gacgaggacg agaaccagag ccccaggagc ttccagaaga agaccaggca ctacttcatt     6180

gctgctgtgg agaggctgtg ggactatggc atgagcagca gcccccatgt gctgaggaac     6240

agggcccagt ctggctctgt gccccagttc aagaaggtgg tgttccagga gttcactgat     6300

ggcagcttca cccagcccct gtacagaggg gagctgaatg agcacctggg cctgctgggc     6360

ccctacatca gggctgaggt ggaggacaac atcatggtga ccttcaggaa ccaggccagc     6420

aggccctaca gcttctacag cagcctgatc agctatgagg aggaccagag gcagggggct     6480

gagcccagga agaactttgt gaagcccaat gaaaccaaga cctacttctg gaaggtgcag     6540

caccacatgg cccccaccaa ggatgagttt gactgcaagg cctgggccta cttctctgat     6600

gtggacctgg agaaggatgt gcactctggc ctgattggcc ccctgctggt gtgccacacc     6660

aacaccctga accctgccca tggcaggcag gtgactgtgc aggagtttgc cctgttcttc     6720

accatctttg atgaaaccaa gagctggtac ttcactgaga acatggagag gaactgcagg     6780

gccccctgca acatccagat ggaggacccc accttcaagg agaactacag gttccatgcc     6840

atcaatggct acatcatgga caccctgcct ggcctggtga tggcccagga ccagaggatc     6900

aggtggtacc tgctgagcat gggcagcaat gagaacatcc acagcatcca cttctctggc     6960

catgtgttca ctgtgaggaa gaaggaggag tacaagatgg ccctgtacaa cctgtaccct     7020

ggggtgtttg agactgtgga gatgctgccc agcaaggctg gcatctggag ggtggagtgc     7080

ctgattgggg agcacctgca tgctggcatg agcaccctgt tcctggtgta cagcaacaag     7140

tgccagaccc ccctgggcat ggcctctggc cacatcaggg acttccagat cactgcctct     7200

ggccagtatg gccagtgggc ccccaagctg gccaggctgc actactctgg cagcatcaat     7260

gcctggagca ccaaggagcc cttcagctgg atcaaggtgg acctgctggc ccccatgatc     7320

atccatggca tcaagaccca gggggccagg cagaagttca gcagcctgta catcagccag     7380

ttcatcatca tgtacagcct ggatggcaag aagtggcaga cctacagggg caacagcact     7440

ggcaccctga tggtgttctt tggcaatgtg gacagctctg gcatcaagca caacatcttc     7500

aaccccccca tcattgccag atacatcagg ctgcacccca cccactacag catcaggagc     7560

accctgagga tggagctgat gggctgtgac ctgaacagct gcagcatgcc cctgggcatg     7620

gagagcaagg ccatctctga tgcccagatc actgccagca gctacttcac caacatgttt     7680

gccacctgga gccccagcaa ggccaggctg cacctgcagg gcaggagcaa tgcctggagg     7740

ccccaggtca acaaccccaa ggagtggctg caggtggact tccagaagac catgaaggtg     7800

actggggtga ccacccaggg ggtgaagagc ctgctgacca gcatgtatgt gaaggagttc     7860

ctgatcagca gcagccagga tggccaccag tggaccctgt tcttccagaa tggcaaggtg     7920

aaggtgttcc agggcaacca ggacagcttc acccctgtgg tgaacagcct ggaccccccc     7980

ctgctgacca gatacctgag gattcacccc cagagctggg tgcaccagat tgccctgagg     8040

atggaggtgc tgggctgtga ggcccaggac ctgtactgag cggccgcggg cccaatcaac     8100

ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta     8160

cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt     8220

tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg     8280

ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg     8340

gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca     8400

cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca     8460

ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg     8520

ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag     8580

cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc     8640

gccctcagac gagtcggatc tccctttggg ccgcctcccc gcaagcttcg cactttttaa     8700

aagaaaaggg aggactggat gggatttatt actccgatag gacgctggct tgtaactcag     8760

tctcttacta ggagaccagc ttgagcctgg gtgttcgctg gttagcctaa cctggttggc     8820

caccaggggt aaggactcct tggcttagaa agctaataaa cttgcctgca ttagagctct     8880

tacgcgtccc gggctcgaga tccgcatctc aattagtcag caaccatagt cccgccccta     8940

actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga     9000

ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag     9060

tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag     9120

cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt     9180

cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtccgc     9240

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca     9300

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg     9360

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     9420

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     9480

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     9540

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     9600

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     9660

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     9720

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     9780

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     9840

cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     9900

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     9960

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt    10020

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag    10080

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat    10140

ctaaagtata tatgagtaaa cttggtctga cagttagaaa aactcatcga gcatcaaatg    10200

aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg    10260

taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc    10320

tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag    10380

gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaacagctt    10440

atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact    10500

cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc    10560

gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag    10620

cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt    10680

tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat    10740

ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc    10800

attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata    10860

caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata    10920

taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat    10980

atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga    11040

tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa caattggtcg    11100

acggatcc                                                             11108


<210>  15
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimised CpG depleted A1AT transgene

<400>  15
atgcccagct ctgtgtcctg gggcattctg ctgctggctg gcctgtgctg tctggtgcct       60

gtgtccctgg ctgaggaccc tcagggggat gctgcccaga aaacagacac ctcccaccat      120

gaccaggacc accccacctt caacaagatc acccccaacc tggcagagtt tgccttcagc      180

ctgtacagac agctggccca ccagagcaac agcaccaaca tctttttcag ccctgtgtcc      240

attgccacag cctttgccat gctgagcctg ggcaccaagg ctgacaccca tgatgagatc      300

ctggaaggcc tgaacttcaa cctgacagag atccctgagg cccagatcca tgagggcttc      360

caggaactgc tgagaaccct gaaccagcca gacagccagc tgcagctgac aacaggcaat      420

gggctgttcc tgtctgaggg cctgaagctg gtggacaagt ttctggaaga tgtgaagaag      480

ctgtaccact ctgaggcctt cacagtgaac tttggggaca cagaagaggc caagaaacag      540

atcaatgact atgtggaaaa gggcacccag ggcaagattg tggaccttgt gaaagagctg      600

gacagggaca ctgtgtttgc ccttgtgaac tacatcttct tcaagggcaa gtgggagagg      660

ccctttgaag tgaaggacac tgaggaagag gacttccatg tggaccaagt gaccacagtg      720

aaggtgccaa tgatgaagag actggggatg ttcaatatcc agcactgcaa gaaactgagc      780

agctgggtgc tgctgatgaa gtacctgggc aatgctacag ccatattctt tctgcctgat      840

gagggcaagc tgcagcacct ggaaaatgag ctgacccatg acatcatcac caaatttctg      900

gaaaatgagg acagaagatc tgccagcctg catctgccca agctgagcat cacaggcaca      960

tatgacctga agtctgtgct gggacagctg ggaatcacca aggtgttcag caatggggca     1020

gacctgagtg gagtgacaga ggaagcccct ctgaagctgt ccaaggctgt gcacaaggca     1080

gtgctgacca ttgatgagaa gggcacagag gctgctgggg ccatgtttct ggaagccatc     1140

cccatgtcca tccccccaga agtgaagttc aacaagccct ttgtgttcct gatgattgag     1200

cagaacacca agagccccct gttcatgggc aaggttgtga accccaccca gaaatga        1257


<210>  16
<211>  5013
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimised CpG depleted FVIII transgene (N6)

<400>  16
atgcagattg agctgagcac ctgcttcttc ctgtgcctgc tgaggttctg cttctctgcc       60

accaggagat actacctggg ggctgtggag ctgagctggg actacatgca gtctgacctg      120

ggggagctgc ctgtggatgc caggttcccc cccagagtgc ccaagagctt ccccttcaac      180

acctctgtgg tgtacaagaa gaccctgttt gtggagttca ctgaccacct gttcaacatt      240

gccaagccca ggcccccctg gatgggcctg ctgggcccca ccatccaggc tgaggtgtat      300

gacactgtgg tgatcaccct gaagaacatg gccagccacc ctgtgagcct gcatgctgtg      360

ggggtgagct actggaaggc ctctgagggg gctgagtatg atgaccagac cagccagagg      420

gagaaggagg atgacaaggt gttccctggg ggcagccaca cctatgtgtg gcaggtgctg      480

aaggagaatg gccccatggc ctctgacccc ctgtgcctga cctacagcta cctgagccat      540

gtggacctgg tgaaggacct gaactctggc ctgattgggg ccctgctggt gtgcagggag      600

ggcagcctgg ccaaggagaa gacccagacc ctgcacaagt tcatcctgct gtttgctgtg      660

tttgatgagg gcaagagctg gcactctgaa accaagaaca gcctgatgca ggacagggat      720

gctgcctctg ccagggcctg gcccaagatg cacactgtga atggctatgt gaacaggagc      780

ctgcctggcc tgattggctg ccacaggaag tctgtgtact ggcatgtgat tggcatgggc      840

accacccctg aggtgcacag catcttcctg gagggccaca ccttcctggt caggaaccac      900

aggcaggcca gcctggagat cagccccatc accttcctga ctgcccagac cctgctgatg      960

gacctgggcc agttcctgct gttctgccac atcagcagcc accagcatga tggcatggag     1020

gcctatgtga aggtggacag ctgccctgag gagccccagc tgaggatgaa gaacaatgag     1080

gaggctgagg actatgatga tgacctgact gactctgaga tggatgtggt gaggtttgat     1140

gatgacaaca gccccagctt catccagatc aggtctgtgg ccaagaagca ccccaagacc     1200

tgggtgcact acattgctgc tgaggaggag gactgggact atgcccccct ggtgctggcc     1260

cctgatgaca ggagctacaa gagccagtac ctgaacaatg gcccccagag gattggcagg     1320

aagtacaaga aggtcaggtt catggcctac actgatgaaa ccttcaagac cagggaggcc     1380

atccagcatg agtctggcat cctgggcccc ctgctgtatg gggaggtggg ggacaccctg     1440

ctgatcatct tcaagaacca ggccagcagg ccctacaaca tctaccccca tggcatcact     1500

gatgtgaggc ccctgtacag caggaggctg cccaaggggg tgaagcacct gaaggacttc     1560

cccatcctgc ctggggagat cttcaagtac aagtggactg tgactgtgga ggatggcccc     1620

accaagtctg accccaggtg cctgaccaga tactacagca gctttgtgaa catggagagg     1680

gacctggcct ctggcctgat tggccccctg ctgatctgct acaaggagtc tgtggaccag     1740

aggggcaacc agatcatgtc tgacaagagg aatgtgatcc tgttctctgt gtttgatgag     1800

aacaggagct ggtacctgac tgagaacatc cagaggttcc tgcccaaccc tgctggggtg     1860

cagctggagg accctgagtt ccaggccagc aacatcatgc acagcatcaa tggctatgtg     1920

tttgacagcc tgcagctgtc tgtgtgcctg catgaggtgg cctactggta catcctgagc     1980

attggggccc agactgactt cctgtctgtg ttcttctctg gctacacctt caagcacaag     2040

atggtgtatg aggacaccct gaccctgttc cccttctctg gggagactgt gttcatgagc     2100

atggagaacc ctggcctgtg gattctgggc tgccacaact ctgacttcag gaacaggggc     2160

atgactgccc tgctgaaagt ctccagctgt gacaagaaca ctggggacta ctatgaggac     2220

agctatgagg acatctctgc ctacctgctg agcaagaaca atgccattga gcccaggagc     2280

ttcagccaga acagcaggca ccccagcacc aggcagaagc agttcaatgc caccaccatc     2340

cctgagaatg acatagagaa gacagaccca tggtttgccc accggacccc catgcccaag     2400

atccagaatg tgagcagctc tgacctgctg atgctgctga ggcagagccc caccccccat     2460

ggcctgagcc tgtctgacct gcaggaggcc aagtatgaaa ccttctctga tgaccccagc     2520

cctggggcca ttgacagcaa caacagcctg tctgagatga cccacttcag gccccagctg     2580

caccactctg gggacatggt gttcacccct gagtctggcc tgcagctgag gctgaatgag     2640

aagctgggca ccactgctgc cactgagctg aagaagctgg acttcaaagt ctccagcacc     2700

agcaacaacc tgatcagcac catcccctct gacaacctgg ctgctggcac tgacaacacc     2760

agcagcctgg gcccccccag catgcctgtg cactatgaca gccagctgga caccaccctg     2820

tttggcaaga agagcagccc cctgactgag tctgggggcc ccctgagcct gtctgaggag     2880

aacaatgaca gcaagctgct ggagtctggc ctgatgaaca gccaggagag cagctggggc     2940

aagaatgtga gcagcaggga gatcaccagg accaccctgc agtctgacca ggaggagatt     3000

gactatgatg acaccatctc tgtggagatg aagaaggagg actttgacat ctacgacgag     3060

gacgagaacc agagccccag gagcttccag aagaagacca ggcactactt cattgctgct     3120

gtggagaggc tgtgggacta tggcatgagc agcagccccc atgtgctgag gaacagggcc     3180

cagtctggct ctgtgcccca gttcaagaag gtggtgttcc aggagttcac tgatggcagc     3240

ttcacccagc ccctgtacag aggggagctg aatgagcacc tgggcctgct gggcccctac     3300

atcagggctg aggtggagga caacatcatg gtgaccttca ggaaccaggc cagcaggccc     3360

tacagcttct acagcagcct gatcagctat gaggaggacc agaggcaggg ggctgagccc     3420

aggaagaact ttgtgaagcc caatgaaacc aagacctact tctggaaggt gcagcaccac     3480

atggccccca ccaaggatga gtttgactgc aaggcctggg cctacttctc tgatgtggac     3540

ctggagaagg atgtgcactc tggcctgatt ggccccctgc tggtgtgcca caccaacacc     3600

ctgaaccctg cccatggcag gcaggtgact gtgcaggagt ttgccctgtt cttcaccatc     3660

tttgatgaaa ccaagagctg gtacttcact gagaacatgg agaggaactg cagggccccc     3720

tgcaacatcc agatggagga ccccaccttc aaggagaact acaggttcca tgccatcaat     3780

ggctacatca tggacaccct gcctggcctg gtgatggccc aggaccagag gatcaggtgg     3840

tacctgctga gcatgggcag caatgagaac atccacagca tccacttctc tggccatgtg     3900

ttcactgtga ggaagaagga ggagtacaag atggccctgt acaacctgta ccctggggtg     3960

tttgagactg tggagatgct gcccagcaag gctggcatct ggagggtgga gtgcctgatt     4020

ggggagcacc tgcatgctgg catgagcacc ctgttcctgg tgtacagcaa caagtgccag     4080

acccccctgg gcatggcctc tggccacatc agggacttcc agatcactgc ctctggccag     4140

tatggccagt gggcccccaa gctggccagg ctgcactact ctggcagcat caatgcctgg     4200

agcaccaagg agcccttcag ctggatcaag gtggacctgc tggcccccat gatcatccat     4260

ggcatcaaga cccagggggc caggcagaag ttcagcagcc tgtacatcag ccagttcatc     4320

atcatgtaca gcctggatgg caagaagtgg cagacctaca ggggcaacag cactggcacc     4380

ctgatggtgt tctttggcaa tgtggacagc tctggcatca agcacaacat cttcaacccc     4440

cccatcattg ccagatacat caggctgcac cccacccact acagcatcag gagcaccctg     4500

aggatggagc tgatgggctg tgacctgaac agctgcagca tgcccctggg catggagagc     4560

aaggccatct ctgatgccca gatcactgcc agcagctact tcaccaacat gtttgccacc     4620

tggagcccca gcaaggccag gctgcacctg cagggcagga gcaatgcctg gaggccccag     4680

gtcaacaacc ccaaggagtg gctgcaggtg gacttccaga agaccatgaa ggtgactggg     4740

gtgaccaccc agggggtgaa gagcctgctg accagcatgt atgtgaagga gttcctgatc     4800

agcagcagcc aggatggcca ccagtggacc ctgttcttcc agaatggcaa ggtgaaggtg     4860

ttccagggca accaggacag cttcacccct gtggtgaaca gcctggaccc ccccctgctg     4920

accagatacc tgaggattca cccccagagc tgggtgcacc agattgccct gaggatggag     4980

gtgctgggct gtgaggccca ggacctgtac tga                                  5013


<210>  17
<211>  873
<212>  DNA
<213>  Cytomegalovirus

<400>  17
ccgcggagat ctcaatattg gccattagcc atattattca ttggttatat agcataaatc       60

aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt      120

ggctcatgtc caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa      180

tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg      240

gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg      300

tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta      360

cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt      420

gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac      480

tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt      540

tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac      600

cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt      660

cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat      720

ataagcagag ctcgtttagt gaaccgtcag atcactagaa gctttattgc ggtagtttat      780

cacagttaaa ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc      840

agaagttggt cgtgaggcac tgggcaggct agc                                   873


<210>  18
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
tcgagatgtg gtctgagtta aaaatcagga gcaacgacgg aggtgaagga ccagacgcca       60

acgaccc                                                                 67


<210>  19
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
ccgggggtcg ttggcgtctg gtccttcacc tccgtcgttg ctcctgattt ttaactcaga       60

ccacatc                                                                 67


<210>  20
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
ccggggaaag ggggtgcaac acatccatat ccagccatct ctacctgttt atggaca          57


<210>  21
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
accctctgtc cataaacagg tagagatggc tggatatgga tgtgttgcac ccctttcc         58


<210>  22
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
gggttaggtg gttgctgatt ctctcattca cccagtggg                              39


<210>  23
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
gatccccact gggtgaatga gagaatcagc aaccaccta                              39


<210>  24
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
gagactcgag atgtggtctg agttaaaaat cagg                                   34


<210>  25
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  25
agaggtagac cagtacgagt cacgtttgcc cctatcacca tccctaaccc tctgtcataa       60

ac                                                                      62


<210>  26
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complementary strand of codon-optimised CpG depleted A1AT 
       transgene

<400>  26
tacgggtcga gacacaggac cccgtaagac gacgaccgac cggacacgac agaccacgga       60

cacagggacc gactcctggg agtcccccta cgacgggtct tttgtctgtg gagggtggta      120

ctggtcctgg tggggtggaa gttgttctag tgggggttgg accgtctcaa acggaagtcg      180

gacatgtctg tcgaccgggt ggtctcgttg tcgtggttgt agaaaaagtc gggacacagg      240

taacggtgtc ggaaacggta cgactcggac ccgtggttcc gactgtgggt actactctag      300

gaccttccgg acttgaagtt ggactgtctc tagggactcc gggtctaggt actcccgaag      360

gtccttgacg actcttggga cttggtcggt ctgtcggtcg acgtcgactg ttgtccgtta      420

cccgacaagg acagactccc ggacttcgac cacctgttca aagaccttct acacttcttc      480

gacatggtga gactccggaa gtgtcacttg aaacccctgt gtcttctccg gttctttgtc      540

tagttactga tacacctttt cccgtgggtc ccgttctaac acctggaaca ctttctcgac      600

ctgtccctgt gacacaaacg ggaacacttg atgtagaaga agttcccgtt caccctctcc      660

gggaaacttc acttcctgtg actccttctc ctgaaggtac acctggttca ctggtgtcac      720

ttccacggtt actacttctc tgacccctac aagttatagg tcgtgacgtt ctttgactcg      780

tcgacccacg acgactactt catggacccg ttacgatgtc ggtataagaa agacggacta      840

ctcccgttcg acgtcgtgga ccttttactc gactgggtac tgtagtagtg gtttaaagac      900

cttttactcc tgtcttctag acggtcggac gtagacgggt tcgactcgta gtgtccgtgt      960

atactggact tcagacacga ccctgtcgac ccttagtggt tccacaagtc gttaccccgt     1020

ctggactcac ctcactgtct ccttcgggga gacttcgaca ggttccgaca cgtgttccgt     1080

cacgactggt aactactctt cccgtgtctc cgacgacccc ggtacaaaga ccttcggtag     1140

gggtacaggt aggggggtct tcacttcaag ttgttcggga aacacaagga ctactaactc     1200

gtcttgtggt tctcggggga caagtacccg ttccaacact tggggtgggt ctttact        1257


<210>  27
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide coded by codon-optimised CpG depleted A1AT transgene

<400>  27

Ala Glu Asp Pro Gln Gly Asp Ala Ala Gln Lys Thr Asp Thr Ser His 
1               5                   10                  15      


His Asp Gln Asp His Pro Thr Phe Ala Glu Asp Pro Gln Gly Asp Ala 
            20                  25                  30          


Ala Gln Lys Thr Asp Thr Ser His His Asp Gln Asp His Pro Thr Phe 
        35                  40                  45              


Asn Lys Ile Thr Pro Asn Leu Ala Glu Phe Ala Phe Ser Leu Tyr Arg 
    50                  55                  60                  


Gln Leu Ala His Gln Ser Asn Ser Thr Asn Ile Phe Phe Ser Pro Val 
65                  70                  75                  80  


Ser Ile Ala Thr Ala Phe Ala Met Leu Ser Leu Gly Thr Lys Ala Asp 
                85                  90                  95      


Thr His Asp Glu Ile Leu Glu Gly Leu Asn Phe Asn Leu Thr Glu Ile 
            100                 105                 110         


Pro Glu Ala Gln Ile His Glu Gly Phe Gln Glu Leu Leu Arg Thr Leu 
        115                 120                 125             


Asn Gln Pro Asp Ser Gln Leu Gln Leu Thr Thr Gly Asn Gly Leu Phe 
    130                 135                 140                 


Leu Ser Glu Gly Leu Lys Leu Val Asp Lys Phe Leu Glu Asp Val Lys 
145                 150                 155                 160 


Lys Leu Tyr His Ser Glu Ala Phe Thr Val Asn Phe Gly Asp Thr Glu 
                165                 170                 175     


Glu Ala Lys Lys Gln Ile Asn Asp Tyr Val Glu Lys Gly Thr Gln Gly 
            180                 185                 190         


Lys Ile Val Asp Leu Val Lys Glu Leu Asp Arg Asp Thr Val Phe Ala 
        195                 200                 205             


Leu Val Asn Tyr Ile Phe Phe Lys Gly Lys Trp Glu Arg Pro Phe Glu 
    210                 215                 220                 


Val Lys Asp Thr Glu Glu Glu Asp Phe His Val Asp Gln Val Thr Thr 
225                 230                 235                 240 


Val Lys Val Pro Met Met Lys Arg Leu Gly Met Phe Asn Ile Gln His 
                245                 250                 255     


Cys Lys Lys Leu Ser Ser Trp Val Leu Leu Met Lys Tyr Leu Gly Asn 
            260                 265                 270         


Ala Thr Ala Ile Phe Phe Leu Pro Asp Glu Gly Lys Leu Gln His Leu 
        275                 280                 285             


Glu Asn Glu Leu Thr His Asp Ile Ile Thr Lys Phe Leu Glu Asn Glu 
    290                 295                 300                 


Asp Arg Arg Ser Ala Ser Leu His Leu Pro Lys Leu Ser Ile Thr Gly 
305                 310                 315                 320 


Thr Tyr Asp Leu Lys Ser Val Leu Gly Gln Leu Gly Ile Thr Lys Val 
                325                 330                 335     


Phe Ser Asn Gly Ala Asp Leu Ser Gly Val Thr Glu Glu Ala Pro Leu 
            340                 345                 350         


Lys Leu Ser Lys Ala Val His Lys Ala Val Leu Thr Ile Asp Glu Lys 
        355                 360                 365             


Gly Thr Glu Ala Ala Gly Ala Met Phe Leu Glu Ala Ile Pro Met Ser 
    370                 375                 380                 


Ile Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met Ile 
385                 390                 395                 400 


Glu Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn Pro 
                405                 410                 415     


Thr Gln Lys 
            


<210>  28
<211>  5013
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complementary strand of codon-optimised CpG depleted FVIII 
       transgene (N6)

<400>  28
tacgtctaac tcgactcgtg gacgaagaag gacacggacg actccaagac gaagagacgg       60

tggtcctcta tgatggaccc ccgacacctc gactcgaccc tgatgtacgt cagactggac      120

cccctcgacg gacacctacg gtccaagggg gggtctcacg ggttctcgaa ggggaagttg      180

tggagacacc acatgttctt ctgggacaaa cacctcaagt gactggtgga caagttgtaa      240

cggttcgggt ccggggggac ctacccggac gacccggggt ggtaggtccg actccacata      300

ctgtgacacc actagtggga cttcttgtac cggtcggtgg gacactcgga cgtacgacac      360

ccccactcga tgaccttccg gagactcccc cgactcatac tactggtctg gtcggtctcc      420

ctcttcctcc tactgttcca caagggaccc ccgtcggtgt ggatacacac cgtccacgac      480

ttcctcttac cggggtaccg gagactgggg gacacggact ggatgtcgat ggactcggta      540

cacctggacc acttcctgga cttgagaccg gactaacccc gggacgacca cacgtccctc      600

ccgtcggacc ggttcctctt ctgggtctgg gacgtgttca agtaggacga caaacgacac      660

aaactactcc cgttctcgac cgtgagactt tggttcttgt cggactacgt cctgtcccta      720

cgacggagac ggtcccggac cgggttctac gtgtgacact taccgataca cttgtcctcg      780

gacggaccgg actaaccgac ggtgtccttc agacacatga ccgtacacta accgtacccg      840

tggtggggac tccacgtgtc gtagaaggac ctcccggtgt ggaaggacca gtccttggtg      900

tccgtccggt cggacctcta gtcggggtag tggaaggact gacgggtctg ggacgactac      960

ctggacccgg tcaaggacga caagacggtg tagtcgtcgg tggtcgtact accgtacctc     1020

cggatacact tccacctgtc gacgggactc ctcggggtcg actcctactt cttgttactc     1080

ctccgactcc tgatactact actggactga ctgagactct acctacacca ctccaaacta     1140

ctactgttgt cggggtcgaa gtaggtctag tccagacacc ggttcttcgt ggggttctgg     1200

acccacgtga tgtaacgacg actcctcctc ctgaccctga tacgggggga ccacgaccgg     1260

ggactactgt cctcgatgtt ctcggtcatg gacttgttac cgggggtctc ctaaccgtcc     1320

ttcatgttct tccagtccaa gtaccggatg tgactacttt ggaagttctg gtccctccgg     1380

taggtcgtac tcagaccgta ggacccgggg gacgacatac ccctccaccc cctgtgggac     1440

gactagtaga agttcttggt ccggtcgtcc gggatgttgt agatgggggt accgtagtga     1500

ctacactccg gggacatgtc gtcctccgac gggttccccc acttcgtgga cttcctgaag     1560

gggtaggacg gacccctcta gaagttcatg ttcacctgac actgacacct cctaccgggg     1620

tggttcagac tggggtccac ggactggtct atgatgtcgt cgaaacactt gtacctctcc     1680

ctggaccgga gaccggacta accgggggac gactagacga tgttcctcag acacctggtc     1740

tccccgttgg tctagtacag actgttctcc ttacactagg acaagagaca caaactactc     1800

ttgtcctcga ccatggactg actcttgtag gtctccaagg acgggttggg acgaccccac     1860

gtcgacctcc tgggactcaa ggtccggtcg ttgtagtacg tgtcgtagtt accgatacac     1920

aaactgtcgg acgtcgacag acacacggac gtactccacc ggatgaccat gtaggactcg     1980

taaccccggg tctgactgaa ggacagacac aagaagagac cgatgtggaa gttcgtgttc     2040

taccacatac tcctgtggga ctgggacaag gggaagagac ccctctgaca caagtactcg     2100

tacctcttgg gaccggacac ctaagacccg acggtgttga gactgaagtc cttgtccccg     2160

tactgacggg acgactttca gaggtcgaca ctgttcttgt gacccctgat gatactcctg     2220

tcgatactcc tgtagagacg gatggacgac tcgttcttgt tacggtaact cgggtcctcg     2280

aagtcggtct tgtcgtccgt ggggtcgtgg tccgtcttcg tcaagttacg gtggtggtag     2340

ggactcttac tgtatctctt ctgtctgggt accaaacggg tggcctgggg gtacgggttc     2400

taggtcttac actcgtcgag actggacgac tacgacgact ccgtctcggg gtggggggta     2460

ccggactcgg acagactgga cgtcctccgg ttcatacttt ggaagagact actggggtcg     2520

ggaccccggt aactgtcgtt gttgtcggac agactctact gggtgaagtc cggggtcgac     2580

gtggtgagac ccctgtacca caagtgggga ctcagaccgg acgtcgactc cgacttactc     2640

ttcgacccgt ggtgacgacg gtgactcgac ttcttcgacc tgaagtttca gaggtcgtgg     2700

tcgttgttgg actagtcgtg gtaggggaga ctgttggacc gacgaccgtg actgttgtgg     2760

tcgtcggacc cgggggggtc gtacggacac gtgatactgt cggtcgacct gtggtgggac     2820

aaaccgttct tctcgtcggg ggactgactc agacccccgg gggactcgga cagactcctc     2880

ttgttactgt cgttcgacga cctcagaccg gactacttgt cggtcctctc gtcgaccccg     2940

ttcttacact cgtcgtccct ctagtggtcc tggtgggacg tcagactggt cctcctctaa     3000

ctgatactac tgtggtagag acacctctac ttcttcctcc tgaaactgta gatgctgctc     3060

ctgctcttgg tctcggggtc ctcgaaggtc ttcttctggt ccgtgatgaa gtaacgacga     3120

cacctctccg acaccctgat accgtactcg tcgtcggggg tacacgactc cttgtcccgg     3180

gtcagaccga gacacggggt caagttcttc caccacaagg tcctcaagtg actaccgtcg     3240

aagtgggtcg gggacatgtc tcccctcgac ttactcgtgg acccggacga cccggggatg     3300

tagtcccgac tccacctcct gttgtagtac cactggaagt ccttggtccg gtcgtccggg     3360

atgtcgaaga tgtcgtcgga ctagtcgata ctcctcctgg tctccgtccc ccgactcggg     3420

tccttcttga aacacttcgg gttactttgg ttctggatga agaccttcca cgtcgtggtg     3480

taccgggggt ggttcctact caaactgacg ttccggaccc ggatgaagag actacacctg     3540

gacctcttcc tacacgtgag accggactaa ccgggggacg accacacggt gtggttgtgg     3600

gacttgggac gggtaccgtc cgtccactga cacgtcctca aacgggacaa gaagtggtag     3660

aaactacttt ggttctcgac catgaagtga ctcttgtacc tctccttgac gtcccggggg     3720

acgttgtagg tctacctcct ggggtggaag ttcctcttga tgtccaaggt acggtagtta     3780

ccgatgtagt acctgtggga cggaccggac cactaccggg tcctggtctc ctagtccacc     3840

atggacgact cgtacccgtc gttactcttg taggtgtcgt aggtgaagag accggtacac     3900

aagtgacact ccttcttcct cctcatgttc taccgggaca tgttggacat gggaccccac     3960

aaactctgac acctctacga cgggtcgttc cgaccgtaga cctcccacct cacggactaa     4020

cccctcgtgg acgtacgacc gtactcgtgg gacaaggacc acatgtcgtt gttcacggtc     4080

tggggggacc cgtaccggag accggtgtag tccctgaagg tctagtgacg gagaccggtc     4140

ataccggtca cccgggggtt cgaccggtcc gacgtgatga gaccgtcgta gttacggacc     4200

tcgtggttcc tcgggaagtc gacctagttc cacctggacg accgggggta ctagtaggta     4260

ccgtagttct gggtcccccg gtccgtcttc aagtcgtcgg acatgtagtc ggtcaagtag     4320

tagtacatgt cggacctacc gttcttcacc gtctggatgt ccccgttgtc gtgaccgtgg     4380

gactaccaca agaaaccgtt acacctgtcg agaccgtagt tcgtgttgta gaagttgggg     4440

gggtagtaac ggtctatgta gtccgacgtg gggtgggtga tgtcgtagtc ctcgtgggac     4500

tcctacctcg actacccgac actggacttg tcgacgtcgt acggggaccc gtacctctcg     4560

ttccggtaga gactacgggt ctagtgacgg tcgtcgatga agtggttgta caaacggtgg     4620

acctcggggt cgttccggtc cgacgtggac gtcccgtcct cgttacggac ctccggggtc     4680

cagttgttgg ggttcctcac cgacgtccac ctgaaggtct tctggtactt ccactgaccc     4740

cactggtggg tcccccactt ctcggacgac tggtcgtaca tacacttcct caaggactag     4800

tcgtcgtcgg tcctaccggt ggtcacctgg gacaagaagg tcttaccgtt ccacttccac     4860

aaggtcccgt tggtcctgtc gaagtgggga caccacttgt cggacctggg gggggacgac     4920

tggtctatgg actcctaagt gggggtctcg acccacgtgg tctaacggga ctcctacctc     4980

cacgacccga cactccgggt cctggacatg act                                  5013


<210>  29
<211>  1670
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide coded by codon-optimised CpG depleted FVIII transgene 
       (N6)

<400>  29

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe 
1               5                   10                  15      


Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser 
            20                  25                  30          


Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg 
        35                  40                  45              


Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val 
    50                  55                  60                  


Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile 
65                  70                  75                  80  


Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln 
                85                  90                  95      


Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser 
            100                 105                 110         


His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser 
        115                 120                 125             


Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp 
    130                 135                 140                 


Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu 
145                 150                 155                 160 


Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser 
                165                 170                 175     


Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile 
            180                 185                 190         


Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr 
        195                 200                 205             


Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly 
    210                 215                 220                 


Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp 
225                 230                 235                 240 


Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr 
                245                 250                 255     


Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val 
            260                 265                 270         


Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile 
        275                 280                 285             


Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser 
    290                 295                 300                 


Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met 
305                 310                 315                 320 


Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His 
                325                 330                 335     


Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro 
            340                 345                 350         


Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp 
        355                 360                 365             


Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser 
    370                 375                 380                 


Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr 
385                 390                 395                 400 


Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro 
                405                 410                 415     


Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn 
            420                 425                 430         


Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met 
        435                 440                 445             


Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu 
    450                 455                 460                 


Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu 
465                 470                 475                 480 


Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro 
                485                 490                 495     


His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys 
            500                 505                 510         


Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe 
        515                 520                 525             


Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp 
    530                 535                 540                 


Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg 
545                 550                 555                 560 


Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu 
                565                 570                 575     


Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val 
            580                 585                 590         


Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu 
        595                 600                 605             


Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp 
    610                 615                 620                 


Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val 
625                 630                 635                 640 


Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp 
                645                 650                 655     


Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe 
            660                 665                 670         


Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr 
        675                 680                 685             


Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro 
    690                 695                 700                 


Gly Leu Trp Ile Leu Gly Cys His Asn Ser Asp Phe Arg Asn Arg Gly 
705                 710                 715                 720 


Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp 
                725                 730                 735     


Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys 
            740                 745                 750         


Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Pro 
        755                 760                 765             


Ser Thr Arg Gln Lys Gln Phe Asn Ala Thr Thr Ile Pro Glu Asn Asp 
    770                 775                 780                 


Ile Glu Lys Thr Asp Pro Trp Phe Ala His Arg Thr Pro Met Pro Lys 
785                 790                 795                 800 


Ile Gln Asn Val Ser Ser Ser Asp Leu Leu Met Leu Leu Arg Gln Ser 
                805                 810                 815     


Pro Thr Pro His Gly Leu Ser Leu Ser Asp Leu Gln Glu Ala Lys Tyr 
            820                 825                 830         


Glu Thr Phe Ser Asp Asp Pro Ser Pro Gly Ala Ile Asp Ser Asn Asn 
        835                 840                 845             


Ser Leu Ser Glu Met Thr His Phe Arg Pro Gln Leu His His Ser Gly 
    850                 855                 860                 


Asp Met Val Phe Thr Pro Glu Ser Gly Leu Gln Leu Arg Leu Asn Glu 
865                 870                 875                 880 


Lys Leu Gly Thr Thr Ala Ala Thr Glu Leu Lys Lys Leu Asp Phe Lys 
                885                 890                 895     


Val Ser Ser Thr Ser Asn Asn Leu Ile Ser Thr Ile Pro Ser Asp Asn 
            900                 905                 910         


Leu Ala Ala Gly Thr Asp Asn Thr Ser Ser Leu Gly Pro Pro Ser Met 
        915                 920                 925             


Pro Val His Tyr Asp Ser Gln Leu Asp Thr Thr Leu Phe Gly Lys Lys 
    930                 935                 940                 


Ser Ser Pro Leu Thr Glu Ser Gly Gly Pro Leu Ser Leu Ser Glu Glu 
945                 950                 955                 960 


Asn Asn Asp Ser Lys Leu Leu Glu Ser Gly Leu Met Asn Ser Gln Glu 
                965                 970                 975     


Ser Ser Trp Gly Lys Asn Val Ser Ser Arg Glu Ile Thr Arg Thr Thr 
            980                 985                 990         


Leu Gln Ser Asp Gln Glu Glu Ile  Asp Tyr Asp Asp Thr  Ile Ser Val 
        995                 1000                 1005             


Glu Met  Lys Lys Glu Asp Phe  Asp Ile Tyr Asp Glu  Asp Glu Asn 
    1010                 1015                 1020             


Gln Ser  Pro Arg Ser Phe Gln  Lys Lys Thr Arg His  Tyr Phe Ile 
    1025                 1030                 1035             


Ala Ala  Val Glu Arg Leu Trp  Asp Tyr Gly Met Ser  Ser Ser Pro 
    1040                 1045                 1050             


His Val  Leu Arg Asn Arg Ala  Gln Ser Gly Ser Val  Pro Gln Phe 
    1055                 1060                 1065             


Lys Lys  Val Val Phe Gln Glu  Phe Thr Asp Gly Ser  Phe Thr Gln 
    1070                 1075                 1080             


Pro Leu  Tyr Arg Gly Glu Leu  Asn Glu His Leu Gly  Leu Leu Gly 
    1085                 1090                 1095             


Pro Tyr  Ile Arg Ala Glu Val  Glu Asp Asn Ile Met  Val Thr Phe 
    1100                 1105                 1110             


Arg Asn  Gln Ala Ser Arg Pro  Tyr Ser Phe Tyr Ser  Ser Leu Ile 
    1115                 1120                 1125             


Ser Tyr  Glu Glu Asp Gln Arg  Gln Gly Ala Glu Pro  Arg Lys Asn 
    1130                 1135                 1140             


Phe Val  Lys Pro Asn Glu Thr  Lys Thr Tyr Phe Trp  Lys Val Gln 
    1145                 1150                 1155             


His His  Met Ala Pro Thr Lys  Asp Glu Phe Asp Cys  Lys Ala Trp 
    1160                 1165                 1170             


Ala Tyr  Phe Ser Asp Val Asp  Leu Glu Lys Asp Val  His Ser Gly 
    1175                 1180                 1185             


Leu Ile  Gly Pro Leu Leu Val  Cys His Thr Asn Thr  Leu Asn Pro 
    1190                 1195                 1200             


Ala His  Gly Arg Gln Val Thr  Val Gln Glu Phe Ala  Leu Phe Phe 
    1205                 1210                 1215             


Thr Ile  Phe Asp Glu Thr Lys  Ser Trp Tyr Phe Thr  Glu Asn Met 
    1220                 1225                 1230             


Glu Arg  Asn Cys Arg Ala Pro  Cys Asn Ile Gln Met  Glu Asp Pro 
    1235                 1240                 1245             


Thr Phe  Lys Glu Asn Tyr Arg  Phe His Ala Ile Asn  Gly Tyr Ile 
    1250                 1255                 1260             


Met Asp  Thr Leu Pro Gly Leu  Val Met Ala Gln Asp  Gln Arg Ile 
    1265                 1270                 1275             


Arg Trp  Tyr Leu Leu Ser Met  Gly Ser Asn Glu Asn  Ile His Ser 
    1280                 1285                 1290             


Ile His  Phe Ser Gly His Val  Phe Thr Val Arg Lys  Lys Glu Glu 
    1295                 1300                 1305             


Tyr Lys  Met Ala Leu Tyr Asn  Leu Tyr Pro Gly Val  Phe Glu Thr 
    1310                 1315                 1320             


Val Glu  Met Leu Pro Ser Lys  Ala Gly Ile Trp Arg  Val Glu Cys 
    1325                 1330                 1335             


Leu Ile  Gly Glu His Leu His  Ala Gly Met Ser Thr  Leu Phe Leu 
    1340                 1345                 1350             


Val Tyr  Ser Asn Lys Cys Gln  Thr Pro Leu Gly Met  Ala Ser Gly 
    1355                 1360                 1365             


His Ile  Arg Asp Phe Gln Ile  Thr Ala Ser Gly Gln  Tyr Gly Gln 
    1370                 1375                 1380             


Trp Ala  Pro Lys Leu Ala Arg  Leu His Tyr Ser Gly  Ser Ile Asn 
    1385                 1390                 1395             


Ala Trp  Ser Thr Lys Glu Pro  Phe Ser Trp Ile Lys  Val Asp Leu 
    1400                 1405                 1410             


Leu Ala  Pro Met Ile Ile His  Gly Ile Lys Thr Gln  Gly Ala Arg 
    1415                 1420                 1425             


Gln Lys  Phe Ser Ser Leu Tyr  Ile Ser Gln Phe Ile  Ile Met Tyr 
    1430                 1435                 1440             


Ser Leu  Asp Gly Lys Lys Trp  Gln Thr Tyr Arg Gly  Asn Ser Thr 
    1445                 1450                 1455             


Gly Thr  Leu Met Val Phe Phe  Gly Asn Val Asp Ser  Ser Gly Ile 
    1460                 1465                 1470             


Lys His  Asn Ile Phe Asn Pro  Pro Ile Ile Ala Arg  Tyr Ile Arg 
    1475                 1480                 1485             


Leu His  Pro Thr His Tyr Ser  Ile Arg Ser Thr Leu  Arg Met Glu 
    1490                 1495                 1500             


Leu Met  Gly Cys Asp Leu Asn  Ser Cys Ser Met Pro  Leu Gly Met 
    1505                 1510                 1515             


Glu Ser  Lys Ala Ile Ser Asp  Ala Gln Ile Thr Ala  Ser Ser Tyr 
    1520                 1525                 1530             


Phe Thr  Asn Met Phe Ala Thr  Trp Ser Pro Ser Lys  Ala Arg Leu 
    1535                 1540                 1545             


His Leu  Gln Gly Arg Ser Asn  Ala Trp Arg Pro Gln  Val Asn Asn 
    1550                 1555                 1560             


Pro Lys  Glu Trp Leu Gln Val  Asp Phe Gln Lys Thr  Met Lys Val 
    1565                 1570                 1575             


Thr Gly  Val Thr Thr Gln Gly  Val Lys Ser Leu Leu  Thr Ser Met 
    1580                 1585                 1590             


Tyr Val  Lys Glu Phe Leu Ile  Ser Ser Ser Gln Asp  Gly His Gln 
    1595                 1600                 1605             


Trp Thr  Leu Phe Phe Gln Asn  Gly Lys Val Lys Val  Phe Gln Gly 
    1610                 1615                 1620             


Asn Gln  Asp Ser Phe Thr Pro  Val Val Asn Ser Leu  Asp Pro Pro 
    1625                 1630                 1635             


Leu Leu  Thr Arg Tyr Leu Arg  Ile His Pro Gln Ser  Trp Val His 
    1640                 1645                 1650             


Gln Ile  Ala Leu Arg Met Glu  Val Leu Gly Cys Glu  Ala Gln Asp 
    1655                 1660                 1665             


Leu Tyr  
    1670 


<210>  30
<211>  4425
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimised CpG depleted FVIII transgene (V3)

<400>  30
atgcagattg agctgagcac ctgcttcttc ctgtgcctgc tgaggttctg cttctctgcc       60

accaggagat actacctggg ggctgtggag ctgagctggg actacatgca gtctgacctg      120

ggggagctgc ctgtggatgc caggttcccc cccagagtgc ccaagagctt ccccttcaac      180

acctctgtgg tgtacaagaa gaccctgttt gtggagttca ctgaccacct gttcaacatt      240

gccaagccca ggcccccctg gatgggcctg ctgggcccca ccatccaggc tgaggtgtat      300

gacactgtgg tgatcaccct gaagaacatg gccagccacc ctgtgagcct gcatgctgtg      360

ggggtgagct actggaaggc ctctgagggg gctgagtatg atgaccagac cagccagagg      420

gagaaggagg atgacaaggt gttccctggg ggcagccaca cctatgtgtg gcaggtgctg      480

aaggagaatg gccccatggc ctctgacccc ctgtgcctga cctacagcta cctgagccat      540

gtggacctgg tgaaggacct gaactctggc ctgattgggg ccctgctggt gtgcagggag      600

ggcagcctgg ccaaggagaa gacccagacc ctgcacaagt tcatcctgct gtttgctgtg      660

tttgatgagg gcaagagctg gcactctgaa accaagaaca gcctgatgca ggacagggat      720

gctgcctctg ccagggcctg gcccaagatg cacactgtga atggctatgt gaacaggagc      780

ctgcctggcc tgattggctg ccacaggaag tctgtgtact ggcatgtgat tggcatgggc      840

accacccctg aggtgcacag catcttcctg gagggccaca ccttcctggt caggaaccac      900

aggcaggcca gcctggagat cagccccatc accttcctga ctgcccagac cctgctgatg      960

gacctgggcc agttcctgct gttctgccac atcagcagcc accagcatga tggcatggag     1020

gcctatgtga aggtggacag ctgccctgag gagccccagc tgaggatgaa gaacaatgag     1080

gaggctgagg actatgatga tgacctgact gactctgaga tggatgtggt gaggtttgat     1140

gatgacaaca gccccagctt catccagatc aggtctgtgg ccaagaagca ccccaagacc     1200

tgggtgcact acattgctgc tgaggaggag gactgggact atgcccccct ggtgctggcc     1260

cctgatgaca ggagctacaa gagccagtac ctgaacaatg gcccccagag gattggcagg     1320

aagtacaaga aggtcaggtt catggcctac actgatgaaa ccttcaagac cagggaggcc     1380

atccagcatg agtctggcat cctgggcccc ctgctgtatg gggaggtggg ggacaccctg     1440

ctgatcatct tcaagaacca ggccagcagg ccctacaaca tctaccccca tggcatcact     1500

gatgtgaggc ccctgtacag caggaggctg cccaaggggg tgaagcacct gaaggacttc     1560

cccatcctgc ctggggagat cttcaagtac aagtggactg tgactgtgga ggatggcccc     1620

accaagtctg accccaggtg cctgaccaga tactacagca gctttgtgaa catggagagg     1680

gacctggcct ctggcctgat tggccccctg ctgatctgct acaaggagtc tgtggaccag     1740

aggggcaacc agatcatgtc tgacaagagg aatgtgatcc tgttctctgt gtttgatgag     1800

aacaggagct ggtacctgac tgagaacatc cagaggttcc tgcccaaccc tgctggggtg     1860

cagctggagg accctgagtt ccaggccagc aacatcatgc acagcatcaa tggctatgtg     1920

tttgacagcc tgcagctgtc tgtgtgcctg catgaggtgg cctactggta catcctgagc     1980

attggggccc agactgactt cctgtctgtg ttcttctctg gctacacctt caagcacaag     2040

atggtgtatg aggacaccct gaccctgttc cccttctctg gggagactgt gttcatgagc     2100

atggagaacc ctggcctgtg gattctgggc tgccacaact ctgacttcag gaacaggggc     2160

atgactgccc tgctgaaagt ctccagctgt gacaagaaca ctggggacta ctatgaggac     2220

agctatgagg acatctctgc ctacctgctg agcaagaaca atgccattga gcccaggagc     2280

ttcagccaga atgccactaa tgtgtctaac aacagcaaca ccagcaatga cagcaatgtg     2340

tctcccccag tgctgaagag gcaccagagg gagatcacca ggaccaccct gcagtctgac     2400

caggaggaga ttgactatga tgacaccatc tctgtggaga tgaagaagga ggactttgac     2460

atctacgacg aggacgagaa ccagagcccc aggagcttcc agaagaagac caggcactac     2520

ttcattgctg ctgtggagag gctgtgggac tatggcatga gcagcagccc ccatgtgctg     2580

aggaacaggg cccagtctgg ctctgtgccc cagttcaaga aggtggtgtt ccaggagttc     2640

actgatggca gcttcaccca gcccctgtac agaggggagc tgaatgagca cctgggcctg     2700

ctgggcccct acatcagggc tgaggtggag gacaacatca tggtgacctt caggaaccag     2760

gccagcaggc cctacagctt ctacagcagc ctgatcagct atgaggagga ccagaggcag     2820

ggggctgagc ccaggaagaa ctttgtgaag cccaatgaaa ccaagaccta cttctggaag     2880

gtgcagcacc acatggcccc caccaaggat gagtttgact gcaaggcctg ggcctacttc     2940

tctgatgtgg acctggagaa ggatgtgcac tctggcctga ttggccccct gctggtgtgc     3000

cacaccaaca ccctgaaccc tgcccatggc aggcaggtga ctgtgcagga gtttgccctg     3060

ttcttcacca tctttgatga aaccaagagc tggtacttca ctgagaacat ggagaggaac     3120

tgcagggccc cctgcaacat ccagatggag gaccccacct tcaaggagaa ctacaggttc     3180

catgccatca atggctacat catggacacc ctgcctggcc tggtgatggc ccaggaccag     3240

aggatcaggt ggtacctgct gagcatgggc agcaatgaga acatccacag catccacttc     3300

tctggccatg tgttcactgt gaggaagaag gaggagtaca agatggccct gtacaacctg     3360

taccctgggg tgtttgagac tgtggagatg ctgcccagca aggctggcat ctggagggtg     3420

gagtgcctga ttggggagca cctgcatgct ggcatgagca ccctgttcct ggtgtacagc     3480

aacaagtgcc agacccccct gggcatggcc tctggccaca tcagggactt ccagatcact     3540

gcctctggcc agtatggcca gtgggccccc aagctggcca ggctgcacta ctctggcagc     3600

atcaatgcct ggagcaccaa ggagcccttc agctggatca aggtggacct gctggccccc     3660

atgatcatcc atggcatcaa gacccagggg gccaggcaga agttcagcag cctgtacatc     3720

agccagttca tcatcatgta cagcctggat ggcaagaagt ggcagaccta caggggcaac     3780

agcactggca ccctgatggt gttctttggc aatgtggaca gctctggcat caagcacaac     3840

atcttcaacc cccccatcat tgccagatac atcaggctgc accccaccca ctacagcatc     3900

aggagcaccc tgaggatgga gctgatgggc tgtgacctga acagctgcag catgcccctg     3960

ggcatggaga gcaaggccat ctctgatgcc cagatcactg ccagcagcta cttcaccaac     4020

atgtttgcca cctggagccc cagcaaggcc aggctgcacc tgcagggcag gagcaatgcc     4080

tggaggcccc aggtcaacaa ccccaaggag tggctgcagg tggacttcca gaagaccatg     4140

aaggtgactg gggtgaccac ccagggggtg aagagcctgc tgaccagcat gtatgtgaag     4200

gagttcctga tcagcagcag ccaggatggc caccagtgga ccctgttctt ccagaatggc     4260

aaggtgaagg tgttccaggg caaccaggac agcttcaccc ctgtggtgaa cagcctggac     4320

ccccccctgc tgaccagata cctgaggatt cacccccaga gctgggtgca ccagattgcc     4380

ctgaggatgg aggtgctggg ctgtgaggcc caggacctgt actga                     4425


<210>  31
<211>  4425
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complementary strand of codon-optimised CpG depleted FVIII 
       transgene (V3)

<400>  31
tacgtctaac tcgactcgtg gacgaagaag gacacggacg actccaagac gaagagacgg       60

tggtcctcta tgatggaccc ccgacacctc gactcgaccc tgatgtacgt cagactggac      120

cccctcgacg gacacctacg gtccaagggg gggtctcacg ggttctcgaa ggggaagttg      180

tggagacacc acatgttctt ctgggacaaa cacctcaagt gactggtgga caagttgtaa      240

cggttcgggt ccggggggac ctacccggac gacccggggt ggtaggtccg actccacata      300

ctgtgacacc actagtggga cttcttgtac cggtcggtgg gacactcgga cgtacgacac      360

ccccactcga tgaccttccg gagactcccc cgactcatac tactggtctg gtcggtctcc      420

ctcttcctcc tactgttcca caagggaccc ccgtcggtgt ggatacacac cgtccacgac      480

ttcctcttac cggggtaccg gagactgggg gacacggact ggatgtcgat ggactcggta      540

cacctggacc acttcctgga cttgagaccg gactaacccc gggacgacca cacgtccctc      600

ccgtcggacc ggttcctctt ctgggtctgg gacgtgttca agtaggacga caaacgacac      660

aaactactcc cgttctcgac cgtgagactt tggttcttgt cggactacgt cctgtcccta      720

cgacggagac ggtcccggac cgggttctac gtgtgacact taccgataca cttgtcctcg      780

gacggaccgg actaaccgac ggtgtccttc agacacatga ccgtacacta accgtacccg      840

tggtggggac tccacgtgtc gtagaaggac ctcccggtgt ggaaggacca gtccttggtg      900

tccgtccggt cggacctcta gtcggggtag tggaaggact gacgggtctg ggacgactac      960

ctggacccgg tcaaggacga caagacggtg tagtcgtcgg tggtcgtact accgtacctc     1020

cggatacact tccacctgtc gacgggactc ctcggggtcg actcctactt cttgttactc     1080

ctccgactcc tgatactact actggactga ctgagactct acctacacca ctccaaacta     1140

ctactgttgt cggggtcgaa gtaggtctag tccagacacc ggttcttcgt ggggttctgg     1200

acccacgtga tgtaacgacg actcctcctc ctgaccctga tacgggggga ccacgaccgg     1260

ggactactgt cctcgatgtt ctcggtcatg gacttgttac cgggggtctc ctaaccgtcc     1320

ttcatgttct tccagtccaa gtaccggatg tgactacttt ggaagttctg gtccctccgg     1380

taggtcgtac tcagaccgta ggacccgggg gacgacatac ccctccaccc cctgtgggac     1440

gactagtaga agttcttggt ccggtcgtcc gggatgttgt agatgggggt accgtagtga     1500

ctacactccg gggacatgtc gtcctccgac gggttccccc acttcgtgga cttcctgaag     1560

gggtaggacg gacccctcta gaagttcatg ttcacctgac actgacacct cctaccgggg     1620

tggttcagac tggggtccac ggactggtct atgatgtcgt cgaaacactt gtacctctcc     1680

ctggaccgga gaccggacta accgggggac gactagacga tgttcctcag acacctggtc     1740

tccccgttgg tctagtacag actgttctcc ttacactagg acaagagaca caaactactc     1800

ttgtcctcga ccatggactg actcttgtag gtctccaagg acgggttggg acgaccccac     1860

gtcgacctcc tgggactcaa ggtccggtcg ttgtagtacg tgtcgtagtt accgatacac     1920

aaactgtcgg acgtcgacag acacacggac gtactccacc ggatgaccat gtaggactcg     1980

taaccccggg tctgactgaa ggacagacac aagaagagac cgatgtggaa gttcgtgttc     2040

taccacatac tcctgtggga ctgggacaag gggaagagac ccctctgaca caagtactcg     2100

tacctcttgg gaccggacac ctaagacccg acggtgttga gactgaagtc cttgtccccg     2160

tactgacggg acgactttca gaggtcgaca ctgttcttgt gacccctgat gatactcctg     2220

tcgatactcc tgtagagacg gatggacgac tcgttcttgt tacggtaact cgggtcctcg     2280

aagtcggtct tacggtgatt acacagattg ttgtcgttgt ggtcgttact gtcgttacac     2340

agagggggtc acgacttctc cgtggtctcc ctctagtggt cctggtggga cgtcagactg     2400

gtcctcctct aactgatact actgtggtag agacacctct acttcttcct cctgaaactg     2460

tagatgctgc tcctgctctt ggtctcgggg tcctcgaagg tcttcttctg gtccgtgatg     2520

aagtaacgac gacacctctc cgacaccctg ataccgtact cgtcgtcggg ggtacacgac     2580

tccttgtccc gggtcagacc gagacacggg gtcaagttct tccaccacaa ggtcctcaag     2640

tgactaccgt cgaagtgggt cggggacatg tctcccctcg acttactcgt ggacccggac     2700

gacccgggga tgtagtcccg actccacctc ctgttgtagt accactggaa gtccttggtc     2760

cggtcgtccg ggatgtcgaa gatgtcgtcg gactagtcga tactcctcct ggtctccgtc     2820

ccccgactcg ggtccttctt gaaacacttc gggttacttt ggttctggat gaagaccttc     2880

cacgtcgtgg tgtaccgggg gtggttccta ctcaaactga cgttccggac ccggatgaag     2940

agactacacc tggacctctt cctacacgtg agaccggact aaccggggga cgaccacacg     3000

gtgtggttgt gggacttggg acgggtaccg tccgtccact gacacgtcct caaacgggac     3060

aagaagtggt agaaactact ttggttctcg accatgaagt gactcttgta cctctccttg     3120

acgtcccggg ggacgttgta ggtctacctc ctggggtgga agttcctctt gatgtccaag     3180

gtacggtagt taccgatgta gtacctgtgg gacggaccgg accactaccg ggtcctggtc     3240

tcctagtcca ccatggacga ctcgtacccg tcgttactct tgtaggtgtc gtaggtgaag     3300

agaccggtac acaagtgaca ctccttcttc ctcctcatgt tctaccggga catgttggac     3360

atgggacccc acaaactctg acacctctac gacgggtcgt tccgaccgta gacctcccac     3420

ctcacggact aacccctcgt ggacgtacga ccgtactcgt gggacaagga ccacatgtcg     3480

ttgttcacgg tctgggggga cccgtaccgg agaccggtgt agtccctgaa ggtctagtga     3540

cggagaccgg tcataccggt cacccggggg ttcgaccggt ccgacgtgat gagaccgtcg     3600

tagttacgga cctcgtggtt cctcgggaag tcgacctagt tccacctgga cgaccggggg     3660

tactagtagg taccgtagtt ctgggtcccc cggtccgtct tcaagtcgtc ggacatgtag     3720

tcggtcaagt agtagtacat gtcggaccta ccgttcttca ccgtctggat gtccccgttg     3780

tcgtgaccgt gggactacca caagaaaccg ttacacctgt cgagaccgta gttcgtgttg     3840

tagaagttgg gggggtagta acggtctatg tagtccgacg tggggtgggt gatgtcgtag     3900

tcctcgtggg actcctacct cgactacccg acactggact tgtcgacgtc gtacggggac     3960

ccgtacctct cgttccggta gagactacgg gtctagtgac ggtcgtcgat gaagtggttg     4020

tacaaacggt ggacctcggg gtcgttccgg tccgacgtgg acgtcccgtc ctcgttacgg     4080

acctccgggg tccagttgtt ggggttcctc accgacgtcc acctgaaggt cttctggtac     4140

ttccactgac cccactggtg ggtcccccac ttctcggacg actggtcgta catacacttc     4200

ctcaaggact agtcgtcgtc ggtcctaccg gtggtcacct gggacaagaa ggtcttaccg     4260

ttccacttcc acaaggtccc gttggtcctg tcgaagtggg gacaccactt gtcggacctg     4320

gggggggacg actggtctat ggactcctaa gtgggggtct cgacccacgt ggtctaacgg     4380

gactcctacc tccacgaccc gacactccgg gtcctggaca tgact                     4425


<210>  32
<211>  1474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide coded by codon-optimised CpG depleted FVIII transgene 
       (V3)

<400>  32

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe 
1               5                   10                  15      


Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser 
            20                  25                  30          


Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg 
        35                  40                  45              


Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val 
    50                  55                  60                  


Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile 
65                  70                  75                  80  


Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln 
                85                  90                  95      


Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser 
            100                 105                 110         


His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser 
        115                 120                 125             


Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp 
    130                 135                 140                 


Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu 
145                 150                 155                 160 


Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser 
                165                 170                 175     


Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile 
            180                 185                 190         


Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr 
        195                 200                 205             


Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly 
    210                 215                 220                 


Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp 
225                 230                 235                 240 


Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr 
                245                 250                 255     


Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val 
            260                 265                 270         


Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile 
        275                 280                 285             


Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser 
    290                 295                 300                 


Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met 
305                 310                 315                 320 


Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His 
                325                 330                 335     


Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro 
            340                 345                 350         


Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp 
        355                 360                 365             


Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser 
    370                 375                 380                 


Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr 
385                 390                 395                 400 


Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro 
                405                 410                 415     


Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn 
            420                 425                 430         


Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met 
        435                 440                 445             


Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu 
    450                 455                 460                 


Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu 
465                 470                 475                 480 


Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro 
                485                 490                 495     


His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys 
            500                 505                 510         


Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe 
        515                 520                 525             


Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp 
    530                 535                 540                 


Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg 
545                 550                 555                 560 


Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu 
                565                 570                 575     


Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val 
            580                 585                 590         


Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu 
        595                 600                 605             


Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp 
    610                 615                 620                 


Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val 
625                 630                 635                 640 


Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp 
                645                 650                 655     


Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe 
            660                 665                 670         


Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr 
        675                 680                 685             


Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro 
    690                 695                 700                 


Gly Leu Trp Ile Leu Gly Cys His Asn Ser Asp Phe Arg Asn Arg Gly 
705                 710                 715                 720 


Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp 
                725                 730                 735     


Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys 
            740                 745                 750         


Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ala Thr Asn Val 
        755                 760                 765             


Ser Asn Asn Ser Asn Thr Ser Asn Asp Ser Asn Val Ser Pro Pro Val 
    770                 775                 780                 


Leu Lys Arg His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp 
785                 790                 795                 800 


Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys 
                805                 810                 815     


Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser 
            820                 825                 830         


Phe Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu 
        835                 840                 845             


Trp Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala 
    850                 855                 860                 


Gln Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe 
865                 870                 875                 880 


Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu 
                885                 890                 895     


His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn 
            900                 905                 910         


Ile Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr 
        915                 920                 925             


Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro 
    930                 935                 940                 


Arg Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys 
945                 950                 955                 960 


Val Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala 
                965                 970                 975     


Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly 
            980                 985                 990         


Leu Ile Gly Pro Leu Leu Val Cys  His Thr Asn Thr Leu  Asn Pro Ala 
        995                 1000                 1005             


His Gly  Arg Gln Val Thr Val  Gln Glu Phe Ala Leu  Phe Phe Thr 
    1010                 1015                 1020             


Ile Phe  Asp Glu Thr Lys Ser  Trp Tyr Phe Thr Glu  Asn Met Glu 
    1025                 1030                 1035             


Arg Asn  Cys Arg Ala Pro Cys  Asn Ile Gln Met Glu  Asp Pro Thr 
    1040                 1045                 1050             


Phe Lys  Glu Asn Tyr Arg Phe  His Ala Ile Asn Gly  Tyr Ile Met 
    1055                 1060                 1065             


Asp Thr  Leu Pro Gly Leu Val  Met Ala Gln Asp Gln  Arg Ile Arg 
    1070                 1075                 1080             


Trp Tyr  Leu Leu Ser Met Gly  Ser Asn Glu Asn Ile  His Ser Ile 
    1085                 1090                 1095             


His Phe  Ser Gly His Val Phe  Thr Val Arg Lys Lys  Glu Glu Tyr 
    1100                 1105                 1110             


Lys Met  Ala Leu Tyr Asn Leu  Tyr Pro Gly Val Phe  Glu Thr Val 
    1115                 1120                 1125             


Glu Met  Leu Pro Ser Lys Ala  Gly Ile Trp Arg Val  Glu Cys Leu 
    1130                 1135                 1140             


Ile Gly  Glu His Leu His Ala  Gly Met Ser Thr Leu  Phe Leu Val 
    1145                 1150                 1155             


Tyr Ser  Asn Lys Cys Gln Thr  Pro Leu Gly Met Ala  Ser Gly His 
    1160                 1165                 1170             


Ile Arg  Asp Phe Gln Ile Thr  Ala Ser Gly Gln Tyr  Gly Gln Trp 
    1175                 1180                 1185             


Ala Pro  Lys Leu Ala Arg Leu  His Tyr Ser Gly Ser  Ile Asn Ala 
    1190                 1195                 1200             


Trp Ser  Thr Lys Glu Pro Phe  Ser Trp Ile Lys Val  Asp Leu Leu 
    1205                 1210                 1215             


Ala Pro  Met Ile Ile His Gly  Ile Lys Thr Gln Gly  Ala Arg Gln 
    1220                 1225                 1230             


Lys Phe  Ser Ser Leu Tyr Ile  Ser Gln Phe Ile Ile  Met Tyr Ser 
    1235                 1240                 1245             


Leu Asp  Gly Lys Lys Trp Gln  Thr Tyr Arg Gly Asn  Ser Thr Gly 
    1250                 1255                 1260             


Thr Leu  Met Val Phe Phe Gly  Asn Val Asp Ser Ser  Gly Ile Lys 
    1265                 1270                 1275             


His Asn  Ile Phe Asn Pro Pro  Ile Ile Ala Arg Tyr  Ile Arg Leu 
    1280                 1285                 1290             


His Pro  Thr His Tyr Ser Ile  Arg Ser Thr Leu Arg  Met Glu Leu 
    1295                 1300                 1305             


Met Gly  Cys Asp Leu Asn Ser  Cys Ser Met Pro Leu  Gly Met Glu 
    1310                 1315                 1320             


Ser Lys  Ala Ile Ser Asp Ala  Gln Ile Thr Ala Ser  Ser Tyr Phe 
    1325                 1330                 1335             


Thr Asn  Met Phe Ala Thr Trp  Ser Pro Ser Lys Ala  Arg Leu His 
    1340                 1345                 1350             


Leu Gln  Gly Arg Ser Asn Ala  Trp Arg Pro Gln Val  Asn Asn Pro 
    1355                 1360                 1365             


Lys Glu  Trp Leu Gln Val Asp  Phe Gln Lys Thr Met  Lys Val Thr 
    1370                 1375                 1380             


Gly Val  Thr Thr Gln Gly Val  Lys Ser Leu Leu Thr  Ser Met Tyr 
    1385                 1390                 1395             


Val Lys  Glu Phe Leu Ile Ser  Ser Ser Gln Asp Gly  His Gln Trp 
    1400                 1405                 1410             


Thr Leu  Phe Phe Gln Asn Gly  Lys Val Lys Val Phe  Gln Gly Asn 
    1415                 1420                 1425             


Gln Asp  Ser Phe Thr Pro Val  Val Asn Ser Leu Asp  Pro Pro Leu 
    1430                 1435                 1440             


Leu Thr  Arg Tyr Leu Arg Ile  His Pro Gln Ser Trp  Val His Gln 
    1445                 1450                 1455             


Ile Ala  Leu Arg Met Glu Val  Leu Gly Cys Glu Ala  Gln Asp Leu 
    1460                 1465                 1470             


Tyr 
    


<210>  33
<211>  873
<212>  DNA
<213>  Cytomegalovirus

<400>  33
ggcgcctcta gagttataac cggtaatcgg tataataagt aaccaatata tcgtatttag       60

ttataaccga taaccggtaa cgtatgcaac atagatatag tattatacat gtaaatataa      120

ccgagtacag gttatactgg cggtacaacc gtaactaata actgatcaat aattatcatt      180

agttaatgcc ccagtaatca agtatcgggt atatacctca aggcgcaatg tattgaatgc      240

catttaccgg gcggaccgac tggcgggttg ctgggggcgg gtaactgcag ttattactgc      300

atacaagggt atcattgcgg ttatccctga aaggtaactg cagttaccca cctcataaat      360

gccatttgac gggtgaaccg tcatgtagtt cacatagtat acggttcagg cgggggataa      420

ctgcagttac tgccatttac cgggcggacc gtaatacggg tcatgtactg gaatgccctg      480

aaaggatgaa ccgtcatgta gatgcataat cagtagcgat aatggtacca ctacgccaaa      540

accgtcatgt ggttacccgc acctatcgcc aaactgagtg cccctaaagg ttcagaggtg      600

gggtaactgc agttaccctc aaacaaaacc gtggttttag ttgccctgaa aggttttaca      660

gcattattgg ggcggggcaa ctgcgtttac ccgccatccg cacatgccac cctccagata      720

tattcgtctc gagcaaatca cttggcagtc tagtgatctt cgaaataacg ccatcaaata      780

gtgtcaattt aacgattgcg tcagtcacga agactgtgtt gtcagagctt gaattcgacg      840

tcttcaacca gcactccgtg acccgtccga tcg                                   873


