                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  LIPASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

<130>  12937-WO-PCT

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1098
<212>  DNA
<213>  Rhizomucor miehei


<220>
<221>  CDS
<222>  (1)..(1098)

<220>
<221>  sig_peptide
<222>  (1)..(72)

<220>
<221>  mat_peptide
<222>  (292)..(1098)

<400>  1
atg gtt ctc aag cag cgt gca aac tac cta gga ttt ctg att gta ttc         48
Met Val Leu Lys Gln Arg Ala Asn Tyr Leu Gly Phe Leu Ile Val Phe           
        -95                 -90                 -85                       

ttc acg gcc ttc ctg gtg gaa gcg gta ccc atc aag aga caa tcg aat         96
Phe Thr Ala Phe Leu Val Glu Ala Val Pro Ile Lys Arg Gln Ser Asn           
    -80                 -75                 -70                           

tcc acg gtc gac agt ctg ccg cct gcc tcg gtc ctc atc ccc tcg aga        144
Ser Thr Val Asp Ser Leu Pro Pro Ala Ser Val Leu Ile Pro Ser Arg           
-65                 -60                 -55                 -50           

acc tcg gca cct tca tca tca cca agc aca acc gac cct gaa gct cca        192
Thr Ser Ala Pro Ser Ser Ser Pro Ser Thr Thr Asp Pro Glu Ala Pro           
                -45                 -40                 -35               

gcc atg agt cgc aat gga ccg ctg ccc tcg gat gta gag act aaa tat        240
Ala Met Ser Arg Asn Gly Pro Leu Pro Ser Asp Val Glu Thr Lys Tyr           
            -30                 -25                 -20                   

ggc atg gct ttg aat gct act tcc tat ccg gat tct gtg gtc caa gca        288
Gly Met Ala Leu Asn Ala Thr Ser Tyr Pro Asp Ser Val Val Gln Ala           
        -15                 -10                 -5                        

atg agc att gat ggt ggt atc cgc gct gcg acc tcg caa gaa atc aat        336
Met Ser Ile Asp Gly Gly Ile Arg Ala Ala Thr Ser Gln Glu Ile Asn           
-1  1               5                   10                  15            

gaa ttg act tat tac act aca cta tct gcc aac tcg tac tgc cgc act        384
Glu Leu Thr Tyr Tyr Thr Thr Leu Ser Ala Asn Ser Tyr Cys Arg Thr           
                20                  25                  30                

gtc att cct gga gct acc tgg gac tgt atc cac tgt gat gca acg gag        432
Val Ile Pro Gly Ala Thr Trp Asp Cys Ile His Cys Asp Ala Thr Glu           
            35                  40                  45                    

gat ctc aag att atc aag act tgg agc acg ctc atc tat gat aca aat        480
Asp Leu Lys Ile Ile Lys Thr Trp Ser Thr Leu Ile Tyr Asp Thr Asn           
        50                  55                  60                        

gca atg gtt gca cgt ggt gac agc gaa aaa act atc tat atc gtt ttc        528
Ala Met Val Ala Arg Gly Asp Ser Glu Lys Thr Ile Tyr Ile Val Phe           
    65                  70                  75                            

cga ggt tcg agc tct atc cgc aac tgg att gct gat ctc acc ttt gtg        576
Arg Gly Ser Ser Ser Ile Arg Asn Trp Ile Ala Asp Leu Thr Phe Val           
80                  85                  90                  95            

cca gtt tca tat cct ccg gtc agt ggt aca aaa gta cac aag gga ttc        624
Pro Val Ser Tyr Pro Pro Val Ser Gly Thr Lys Val His Lys Gly Phe           
                100                 105                 110               

ctg gac agt tac ggg gaa gtt caa aac gag ctt gtt gct act gtt ctt        672
Leu Asp Ser Tyr Gly Glu Val Gln Asn Glu Leu Val Ala Thr Val Leu           
            115                 120                 125                   

gat caa ttc aag caa tat cca agc tac aag gtt gct gtt aca ggt cac        720
Asp Gln Phe Lys Gln Tyr Pro Ser Tyr Lys Val Ala Val Thr Gly His           
        130                 135                 140                       

tca ctc ggt ggt gct act gcg ttg ctt tgc gcc ctg gat ctc tat caa        768
Ser Leu Gly Gly Ala Thr Ala Leu Leu Cys Ala Leu Asp Leu Tyr Gln           
    145                 150                 155                           

cga gaa gaa gga ctc tca tcc agc aac ttg ttc ctt tac act caa ggt        816
Arg Glu Glu Gly Leu Ser Ser Ser Asn Leu Phe Leu Tyr Thr Gln Gly           
160                 165                 170                 175           

caa cca cgg gta ggc gac cct gcc ttt gcc aac tac gtt gtt agc acc        864
Gln Pro Arg Val Gly Asp Pro Ala Phe Ala Asn Tyr Val Val Ser Thr           
                180                 185                 190               

ggc att cct tac agg cgc acg gtc aat gaa cga gat atc gtt cct cat        912
Gly Ile Pro Tyr Arg Arg Thr Val Asn Glu Arg Asp Ile Val Pro His           
            195                 200                 205                   

ctt cca cct gct gct ttt ggt ttt ctc cac gct ggc gag gag tat tgg        960
Leu Pro Pro Ala Ala Phe Gly Phe Leu His Ala Gly Glu Glu Tyr Trp           
        210                 215                 220                       

att act gac aat agc cca gag act gtt cag gtc tgc aca agc gat ctg       1008
Ile Thr Asp Asn Ser Pro Glu Thr Val Gln Val Cys Thr Ser Asp Leu           
    225                 230                 235                           

gaa acc tct gat tgc tct aac agc att gtt ccc ttc aca agt gtt ctt       1056
Glu Thr Ser Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Val Leu           
240                 245                 250                 255           

gac cat ctc tcg tac ttt ggt atc aac aca ggc ctc tgt act               1098
Asp His Leu Ser Tyr Phe Gly Ile Asn Thr Gly Leu Cys Thr                   
                260                 265                                   


<210>  2
<211>  366
<212>  PRT
<213>  Rhizomucor miehei

<400>  2

Met Val Leu Lys Gln Arg Ala Asn Tyr Leu Gly Phe Leu Ile Val Phe 
        -95                 -90                 -85             


Phe Thr Ala Phe Leu Val Glu Ala Val Pro Ile Lys Arg Gln Ser Asn 
    -80                 -75                 -70                 


Ser Thr Val Asp Ser Leu Pro Pro Ala Ser Val Leu Ile Pro Ser Arg 
-65                 -60                 -55                 -50 


Thr Ser Ala Pro Ser Ser Ser Pro Ser Thr Thr Asp Pro Glu Ala Pro 
                -45                 -40                 -35     


Ala Met Ser Arg Asn Gly Pro Leu Pro Ser Asp Val Glu Thr Lys Tyr 
            -30                 -25                 -20         


Gly Met Ala Leu Asn Ala Thr Ser Tyr Pro Asp Ser Val Val Gln Ala 
        -15                 -10                 -5              


Met Ser Ile Asp Gly Gly Ile Arg Ala Ala Thr Ser Gln Glu Ile Asn 
-1  1               5                   10                  15  


Glu Leu Thr Tyr Tyr Thr Thr Leu Ser Ala Asn Ser Tyr Cys Arg Thr 
                20                  25                  30      


Val Ile Pro Gly Ala Thr Trp Asp Cys Ile His Cys Asp Ala Thr Glu 
            35                  40                  45          


Asp Leu Lys Ile Ile Lys Thr Trp Ser Thr Leu Ile Tyr Asp Thr Asn 
        50                  55                  60              


Ala Met Val Ala Arg Gly Asp Ser Glu Lys Thr Ile Tyr Ile Val Phe 
    65                  70                  75                  


Arg Gly Ser Ser Ser Ile Arg Asn Trp Ile Ala Asp Leu Thr Phe Val 
80                  85                  90                  95  


Pro Val Ser Tyr Pro Pro Val Ser Gly Thr Lys Val His Lys Gly Phe 
                100                 105                 110     


Leu Asp Ser Tyr Gly Glu Val Gln Asn Glu Leu Val Ala Thr Val Leu 
            115                 120                 125         


Asp Gln Phe Lys Gln Tyr Pro Ser Tyr Lys Val Ala Val Thr Gly His 
        130                 135                 140             


Ser Leu Gly Gly Ala Thr Ala Leu Leu Cys Ala Leu Asp Leu Tyr Gln 
    145                 150                 155                 


Arg Glu Glu Gly Leu Ser Ser Ser Asn Leu Phe Leu Tyr Thr Gln Gly 
160                 165                 170                 175 


Gln Pro Arg Val Gly Asp Pro Ala Phe Ala Asn Tyr Val Val Ser Thr 
                180                 185                 190     


Gly Ile Pro Tyr Arg Arg Thr Val Asn Glu Arg Asp Ile Val Pro His 
            195                 200                 205         


Leu Pro Pro Ala Ala Phe Gly Phe Leu His Ala Gly Glu Glu Tyr Trp 
        210                 215                 220             


Ile Thr Asp Asn Ser Pro Glu Thr Val Gln Val Cys Thr Ser Asp Leu 
    225                 230                 235                 


Glu Thr Ser Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Val Leu 
240                 245                 250                 255 


Asp His Leu Ser Tyr Phe Gly Ile Asn Thr Gly Leu Cys Thr 
                260                 265                 


<210>  3
<211>  269
<212>  PRT
<213>  Rhizomucor miehei

<400>  3

Ser Ile Asp Gly Gly Ile Arg Ala Ala Thr Ser Gln Glu Ile Asn Glu 
1               5                   10                  15      


Leu Thr Tyr Tyr Thr Thr Leu Ser Ala Asn Ser Tyr Cys Arg Thr Val 
            20                  25                  30          


Ile Pro Gly Ala Thr Trp Asp Cys Ile His Cys Asp Ala Thr Glu Asp 
        35                  40                  45              


Leu Lys Ile Ile Lys Thr Trp Ser Thr Leu Ile Tyr Asp Thr Asn Ala 
    50                  55                  60                  


Met Val Ala Arg Gly Asp Ser Glu Lys Thr Ile Tyr Ile Val Phe Arg 
65                  70                  75                  80  


Gly Ser Ser Ser Ile Arg Asn Trp Ile Ala Asp Leu Thr Phe Val Pro 
                85                  90                  95      


Val Ser Tyr Pro Pro Val Ser Gly Thr Lys Val His Lys Gly Phe Leu 
            100                 105                 110         


Asp Ser Tyr Gly Glu Val Gln Asn Glu Leu Val Ala Thr Val Leu Asp 
        115                 120                 125             


Gln Phe Lys Gln Tyr Pro Ser Tyr Lys Val Ala Val Thr Gly His Ser 
    130                 135                 140                 


Leu Gly Gly Ala Thr Ala Leu Leu Cys Ala Leu Asp Leu Tyr Gln Arg 
145                 150                 155                 160 


Glu Glu Gly Leu Ser Ser Ser Asn Leu Phe Leu Tyr Thr Gln Gly Gln 
                165                 170                 175     


Pro Arg Val Gly Asp Pro Ala Phe Ala Asn Tyr Val Val Ser Thr Gly 
            180                 185                 190         


Ile Pro Tyr Arg Arg Thr Val Asn Glu Arg Asp Ile Val Pro His Leu 
        195                 200                 205             


Pro Pro Ala Ala Phe Gly Phe Leu His Ala Gly Glu Glu Tyr Trp Ile 
    210                 215                 220                 


Thr Asp Asn Ser Pro Glu Thr Val Gln Val Cys Thr Ser Asp Leu Glu 
225                 230                 235                 240 


Thr Ser Asp Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Val Leu Asp 
                245                 250                 255     


His Leu Ser Tyr Phe Gly Ile Asn Thr Gly Leu Cys Thr 
            260                 265                 


