                         SEQUENCE LISTING

<110>  E.I. duPont de Nemours and Company, Inc.
       DiCosimo, Robert
       Eliot, Andrew
       Ouwehand, Arthur
       Ruebling-Jass, Kristin
       Roesch, Brian
       Rothman, Steve
       You, Zheng
       Cheng, Qiong
 
<120>  ENZYMATIC SYNTHESIS OF SOLUBLE GLUCAN FIBER

<130>  CL5914PCT

<150>  US 62/004300

<151>  2014-05-29

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  3855
<212>  DNA
<213>  Gluconobacter oxydans

<400>  1
atggctgaca actctgacga gcaattcgta gcgtccgatt acacgctgct tggtgacgcg       60

accgacgacc ccaacggtat ggtcgacatg ccggccggtc aggagccagc cacgaccaac      120

gcggttccgt cgggcgtcga atacaatttc ctcgccgcga cagcggggta ttacacggtc      180

agctttgcct atcagaatac ggcgaacgcg gctgcttacg aacagctttc catcaatggc      240

cagaacgagc ccggggtcgt cgaattcgac cagaccagcg gcgcctcgac gggcacggcc      300

tatgcgtcag tctatctgaa ggcaggcctg aattcggtcg aactgaataa tcagacgacc      360

gatgagacca atggcctgac accggtgccg gaggatcagc ttcaggcaag ccccgccttc      420

cagatcggca cgtccacggt cgcggccggc gcatcgccct cccaggaaac gtcggcgcag      480

tcgctcgcga tctccaatca gacggatatg caggcgttcg ttcaaggcga gagcatggcg      540

cctaagcagc aatggacatt cggcccgagc ctgtccgaac tgcatgtcgg cagcaacgac      600

gaactcaatc agctcgactt caacgccgtc tggttccgca acgtcacgcc cggccagcag      660

gccgagactt attcgccgta tttcaaatcc aacgagtcgt tcgatgccaa cggcgtcctg      720

catgtgaact atggtgccta ctcgccgacc ggccaggcgc tgccggtgca gatccaggca      780

acctatgcca acgttccgaa cgaaaacctc atcgtcgaaa acctgtccct gaccaatcag      840

aatgcgagcg gcacccagcc gctggtctgg gacgtgatga atgccaccgg tatcaaccca      900

ggtgaagtca gcagcacgac atgggatccg acgcataacg catggatcgt taccgaggac      960

cagggaagtg gcaaatcccc gctttatcta gcgatcggtg actatcaggt cagcaacagc     1020

acgcacgcgg caggcgtcga cggcgtcaat gtgcgcggca gctattccct gtcgagcggc     1080

gcgtccggca atccgtccct gaatgctccg gaccagggcg ttatcggcgg gttcgagaac     1140

aacggcacga tggtcggcag cagttcgggc gcgtcgggca caaatctcgc ggtcggcacc     1200

accgacagcg atgtcaccct gaatcccggc cagaccgtcg acctcagcta ctatctctcg     1260

acagccacca gcctgtcgca gctcgacgcc aatctcgata aatatacgaa cacagtcggg     1320

tccacgacgt cgtcgacgcc gatgacggac gccaccggcg cttccgccgc gagttcctgg     1380

actcagcaga cggctaccgc atgggacgac acgctcaacc aggcctacaa tttggctggg     1440

tcgacagaaa caagcggaca ggctgcaact gccgccagcg ggcagacact ggatcctaca     1500

agcagcgctg cggcccagag tgcctatcgc tccagtctga tcaatatttt gcaggcacag     1560

agcccggaat acggctcgtt catggcgtcc accaacccgt cctatgaata caaggtctgg     1620

gtgcgcgaca gcgccgcaac cgcgatcggt ctggacgacg caggcctgac ccaaccggcc     1680

gacaagttct ggcgctggat ggcgtccgtc gagcagaacg gcatgaatgc gacctatagc     1740

ggcaacgctt caggcacatt ctcgacgaac tatggagaat tcgaccagaa cctgccgatc     1800

gggttcgtcg cgccggaaaa cgactctcag ggcctgttcc tcatcgggtc ttaccgtcat     1860

tacgagcaga tgctcagcga gggccagact cagcaggccc agtccttcat cagcgatccg     1920

acggtgcgtc aggctctggt caactccgcg aactggatcc aggaaaatat tggtagcaac     1980

gggcttgggc cggctgacta ctcaatctgg gaggacatgt acggctatca tactttcacg     2040

caggtcacct acgcggaagg gctgaacgca gcatcgcaac tcgcctcggc catgggcgag     2100

ggcaatcagg cccagacctg ggcgaccggc gccgagacga tcaaggatgc gatcctgcgt     2160

ccgaccacgg cgtcgacgcc ggggctctgg aatgcgcagg aaggtcattt cgtcgaaatg     2220

atcaaccaga acggaacgat tgacaatacg atcgacgccg ataccaacat tgccgccgtc     2280

ttcggtcttg tctcgcccac aagcatctat gcgactgaaa acgcgcaggc tgtcgaaaat     2340

gcgctgacgc aggacaattt cggtctatcg cgctatcaga acgaaacatt ctaccagtcc     2400

tcgcagtgga gccctggggg cacatacgag gcgcaaggca tctcgccgtc ctggccgcag     2460

atgaccgcct atgacagcat cgtcagcatg gattccggca atacgaccca ggcaaataac     2520

gaccttagct ggatcgaaca gtcctatgac aatggcggca ctccgcccgg tgaatcctac     2580

gactgggcgc gtggccagcc gatcgaaagc acatcgtccg aaccggtgac ggcgagctgg     2640

tacgtccagg atctcctgaa cagcaccggc cagacatcga ccctgatgcc tgcgattacc     2700

gggcaggcac cgaccgctac gccgcagacc gacgtctccg tcaatacagg cacgaccggg     2760

ttcgggtcct acacccttgg actcgacagc gtgggtaaca gccaggccca ggatatgatc     2820

gtcggcacgg gcaacacctc tgccacgtcg gtcgccatgg tccgcaatga agctgctgcc     2880

ggtacaccgg aaaccatcga caacacgaac ggtgtcaacg accttctggt ctttggcaat     2940

gccggcgata cgaacgttct cgccgggcag aattccacta cgacaatcat gaacaacgcc     3000

gccggggacc atggcacggt ggaatacacc ggggctacgg gtgccagtgc gacgattctc     3060

gccggacctc tgacggaaat tcaggccgcc ggtacgacaa acctgatcat gggatcgcaa     3120

tccgatacga cgacgtccga tgcctatatc acgggtggcc agtacgacag tgccgaccag     3180

accaacatca ccactgaggg cccggcagga caactggacg cgatcgacaa tcaaggcaac     3240

gcacatacga cgcttcaggt cgcaagtccg accgacctcc tctataccgg ccgcgacacc     3300

acgttgaatc ttggcactga cggccagaag tcgaccatca actctcttgg taacgaccag     3360

attcatatga acggatcgaa cgtgaatgtg gcgtccgtca ccggagggtt cgacacgttc     3420

tatggtggca ccggcaccat gaccatcaac gccagccagt cggtcggcta tacgcaggaa     3480

acctatatcg gcagccagac gtccggcggg agcctgacct atacaggcgg caacgctgcg     3540

aaccagatca cgctgggcga cgaaagcagc gttgcgatca cggccggcgc cggggctatg     3600

aacatcaccg caaacgacag caccggtctt tgggccaacc tgacgaacgc ggcatcggcg     3660

gacctgaact ttgggtccag ccttggcaac tctatgatct acggcttcaa cggaagcact     3720

gacagtgcga ccatgcaggg tgtcaccggg acctccttct cggggggcaa cctgatggtc     3780

agcctggctg acaatcacag catcacgttc ttcgacgtcc atacccttca gggcatgaac     3840

atcgtcggcg cctaa                                                      3855


<210>  2
<211>  1284
<212>  PRT
<213>  Gluconobacter oxydans

<400>  2

Met Ala Asp Asn Ser Asp Glu Gln Phe Val Ala Ser Asp Tyr Thr Leu 
1               5                   10                  15      


Leu Gly Asp Ala Thr Asp Asp Pro Asn Gly Met Val Asp Met Pro Ala 
            20                  25                  30          


Gly Gln Glu Pro Ala Thr Thr Asn Ala Val Pro Ser Gly Val Glu Tyr 
        35                  40                  45              


Asn Phe Leu Ala Ala Thr Ala Gly Tyr Tyr Thr Val Ser Phe Ala Tyr 
    50                  55                  60                  


Gln Asn Thr Ala Asn Ala Ala Ala Tyr Glu Gln Leu Ser Ile Asn Gly 
65                  70                  75                  80  


Gln Asn Glu Pro Gly Val Val Glu Phe Asp Gln Thr Ser Gly Ala Ser 
                85                  90                  95      


Thr Gly Thr Ala Tyr Ala Ser Val Tyr Leu Lys Ala Gly Leu Asn Ser 
            100                 105                 110         


Val Glu Leu Asn Asn Gln Thr Thr Asp Glu Thr Asn Gly Leu Thr Pro 
        115                 120                 125             


Val Pro Glu Asp Gln Leu Gln Ala Ser Pro Ala Phe Gln Ile Gly Thr 
    130                 135                 140                 


Ser Thr Val Ala Ala Gly Ala Ser Pro Ser Gln Glu Thr Ser Ala Gln 
145                 150                 155                 160 


Ser Leu Ala Ile Ser Asn Gln Thr Asp Met Gln Ala Phe Val Gln Gly 
                165                 170                 175     


Glu Ser Met Ala Pro Lys Gln Gln Trp Thr Phe Gly Pro Ser Leu Ser 
            180                 185                 190         


Glu Leu His Val Gly Ser Asn Asp Glu Leu Asn Gln Leu Asp Phe Asn 
        195                 200                 205             


Ala Val Trp Phe Arg Asn Val Thr Pro Gly Gln Gln Ala Glu Thr Tyr 
    210                 215                 220                 


Ser Pro Tyr Phe Lys Ser Asn Glu Ser Phe Asp Ala Asn Gly Val Leu 
225                 230                 235                 240 


His Val Asn Tyr Gly Ala Tyr Ser Pro Thr Gly Gln Ala Leu Pro Val 
                245                 250                 255     


Gln Ile Gln Ala Thr Tyr Ala Asn Val Pro Asn Glu Asn Leu Ile Val 
            260                 265                 270         


Glu Asn Leu Ser Leu Thr Asn Gln Asn Ala Ser Gly Thr Gln Pro Leu 
        275                 280                 285             


Val Trp Asp Val Met Asn Ala Thr Gly Ile Asn Pro Gly Glu Val Ser 
    290                 295                 300                 


Ser Thr Thr Trp Asp Pro Thr His Asn Ala Trp Ile Val Thr Glu Asp 
305                 310                 315                 320 


Gln Gly Ser Gly Lys Ser Pro Leu Tyr Leu Ala Ile Gly Asp Tyr Gln 
                325                 330                 335     


Val Ser Asn Ser Thr His Ala Ala Gly Val Asp Gly Val Asn Val Arg 
            340                 345                 350         


Gly Ser Tyr Ser Leu Ser Ser Gly Ala Ser Gly Asn Pro Ser Leu Asn 
        355                 360                 365             


Ala Pro Asp Gln Gly Val Ile Gly Gly Phe Glu Asn Asn Gly Thr Met 
    370                 375                 380                 


Val Gly Ser Ser Ser Gly Ala Ser Gly Thr Asn Leu Ala Val Gly Thr 
385                 390                 395                 400 


Thr Asp Ser Asp Val Thr Leu Asn Pro Gly Gln Thr Val Asp Leu Ser 
                405                 410                 415     


Tyr Tyr Leu Ser Thr Ala Thr Ser Leu Ser Gln Leu Asp Ala Asn Leu 
            420                 425                 430         


Asp Lys Tyr Thr Asn Thr Val Gly Ser Thr Thr Ser Ser Thr Pro Met 
        435                 440                 445             


Thr Asp Ala Thr Gly Ala Ser Ala Ala Ser Ser Trp Thr Gln Gln Thr 
    450                 455                 460                 


Ala Thr Ala Trp Asp Asp Thr Leu Asn Gln Ala Tyr Asn Leu Ala Gly 
465                 470                 475                 480 


Ser Thr Glu Thr Ser Gly Gln Ala Ala Thr Ala Ala Ser Gly Gln Thr 
                485                 490                 495     


Leu Asp Pro Thr Ser Ser Ala Ala Ala Gln Ser Ala Tyr Arg Ser Ser 
            500                 505                 510         


Leu Ile Asn Ile Leu Gln Ala Gln Ser Pro Glu Tyr Gly Ser Phe Met 
        515                 520                 525             


Ala Ser Thr Asn Pro Ser Tyr Glu Tyr Lys Val Trp Val Arg Asp Ser 
    530                 535                 540                 


Ala Ala Thr Ala Ile Gly Leu Asp Asp Ala Gly Leu Thr Gln Pro Ala 
545                 550                 555                 560 


Asp Lys Phe Trp Arg Trp Met Ala Ser Val Glu Gln Asn Gly Met Asn 
                565                 570                 575     


Ala Thr Tyr Ser Gly Asn Ala Ser Gly Thr Phe Ser Thr Asn Tyr Gly 
            580                 585                 590         


Glu Phe Asp Gln Asn Leu Pro Ile Gly Phe Val Ala Pro Glu Asn Asp 
        595                 600                 605             


Ser Gln Gly Leu Phe Leu Ile Gly Ser Tyr Arg His Tyr Glu Gln Met 
    610                 615                 620                 


Leu Ser Glu Gly Gln Thr Gln Gln Ala Gln Ser Phe Ile Ser Asp Pro 
625                 630                 635                 640 


Thr Val Arg Gln Ala Leu Val Asn Ser Ala Asn Trp Ile Gln Glu Asn 
                645                 650                 655     


Ile Gly Ser Asn Gly Leu Gly Pro Ala Asp Tyr Ser Ile Trp Glu Asp 
            660                 665                 670         


Met Tyr Gly Tyr His Thr Phe Thr Gln Val Thr Tyr Ala Glu Gly Leu 
        675                 680                 685             


Asn Ala Ala Ser Gln Leu Ala Ser Ala Met Gly Glu Gly Asn Gln Ala 
    690                 695                 700                 


Gln Thr Trp Ala Thr Gly Ala Glu Thr Ile Lys Asp Ala Ile Leu Arg 
705                 710                 715                 720 


Pro Thr Thr Ala Ser Thr Pro Gly Leu Trp Asn Ala Gln Glu Gly His 
                725                 730                 735     


Phe Val Glu Met Ile Asn Pro Asn Gly Thr Ile Asp Asn Thr Ile Asp 
            740                 745                 750         


Ala Asp Thr Asn Ile Ala Ala Val Phe Gly Leu Val Ser Pro Thr Ser 
        755                 760                 765             


Ile Tyr Ala Thr Glu Asn Ala Gln Ala Val Glu Asn Ala Leu Thr Gln 
    770                 775                 780                 


Asp Asn Phe Gly Leu Ser Arg Tyr Gln Asn Glu Thr Phe Tyr Gln Ser 
785                 790                 795                 800 


Ser Gln Trp Ser Pro Gly Gly Thr Tyr Glu Ala Gln Gly Ile Ser Pro 
                805                 810                 815     


Ser Trp Pro Gln Met Thr Ala Tyr Asp Ser Ile Val Ser Met Asp Ser 
            820                 825                 830         


Gly Asn Thr Thr Gln Ala Asn Asn Asp Leu Ser Trp Ile Glu Gln Ser 
        835                 840                 845             


Tyr Asp Asn Gly Gly Thr Pro Pro Gly Glu Ser Tyr Asp Trp Ala Arg 
    850                 855                 860                 


Gly Gln Pro Ile Glu Ser Thr Ser Ser Glu Pro Val Thr Ala Ser Trp 
865                 870                 875                 880 


Tyr Val Gln Asp Leu Leu Asn Ser Thr Gly Gln Thr Ser Thr Leu Met 
                885                 890                 895     


Pro Ala Ile Thr Gly Gln Ala Pro Thr Ala Thr Pro Gln Thr Asp Val 
            900                 905                 910         


Ser Val Asn Thr Gly Thr Thr Gly Phe Gly Ser Tyr Thr Leu Gly Leu 
        915                 920                 925             


Asp Ser Val Gly Asn Ser Gln Ala Gln Asp Met Ile Val Gly Thr Gly 
    930                 935                 940                 


Asn Thr Ser Ala Thr Ser Val Ala Met Val Arg Asn Glu Ala Ala Ala 
945                 950                 955                 960 


Gly Thr Pro Glu Thr Ile Asp Asn Thr Asn Gly Val Asn Asp Leu Leu 
                965                 970                 975     


Val Phe Gly Asn Ala Gly Asp Thr Asn Val Leu Ala Gly Gln Asn Ser 
            980                 985                 990         


Thr Thr Thr Ile Met Asn Asn Ala  Ala Gly Asp His Gly  Thr Val Glu 
        995                 1000                 1005             


Tyr Thr  Gly Ala Thr Gly Ala  Ser Ala Thr Ile Leu  Ala Gly Pro 
    1010                 1015                 1020             


Leu Thr  Glu Ile Gln Ala Ala  Gly Thr Thr Asn Leu  Ile Met Gly 
    1025                 1030                 1035             


Ser Gln  Ser Asp Thr Thr Thr  Ser Asp Ala Tyr Ile  Thr Gly Gly 
    1040                 1045                 1050             


Gln Tyr  Asp Ser Ala Asp Gln  Thr Asn Ile Thr Thr  Glu Gly Pro 
    1055                 1060                 1065             


Ala Gly  Gln Leu Asp Ala Ile  Asp Asn Gln Gly Asn  Ala His Thr 
    1070                 1075                 1080             


Thr Leu  Gln Val Ala Ser Pro  Thr Asp Leu Leu Tyr  Thr Gly Arg 
    1085                 1090                 1095             


Asp Thr  Thr Leu Asn Leu Gly  Thr Asp Gly Gln Lys  Ser Thr Ile 
    1100                 1105                 1110             


Asn Ser  Leu Gly Asn Asp Gln  Ile His Met Asn Gly  Ser Asn Val 
    1115                 1120                 1125             


Asn Val  Ala Ser Val Thr Gly  Gly Phe Asp Thr Phe  Tyr Gly Gly 
    1130                 1135                 1140             


Thr Gly  Thr Met Thr Ile Asn  Ala Ser Gln Ser Val  Gly Tyr Thr 
    1145                 1150                 1155             


Gln Glu  Thr Tyr Ile Gly Ser  Gln Thr Ser Gly Gly  Ser Leu Thr 
    1160                 1165                 1170             


Tyr Thr  Gly Gly Asn Ala Ala  Asn Gln Ile Thr Leu  Gly Asp Glu 
    1175                 1180                 1185             


Ser Ser  Val Ala Ile Thr Ala  Gly Ala Gly Ala Met  Asn Ile Thr 
    1190                 1195                 1200             


Ala Asn  Asp Ser Thr Gly Leu  Trp Ala Asn Leu Thr  Asn Ala Ala 
    1205                 1210                 1215             


Ser Ala  Asp Leu Asn Phe Gly  Ser Ser Leu Gly Asn  Ser Met Ile 
    1220                 1225                 1230             


Tyr Gly  Phe Asn Gly Ser Thr  Asp Ser Ala Thr Met  Gln Gly Val 
    1235                 1240                 1245             


Thr Gly  Thr Ser Phe Ser Gly  Gly Asn Leu Met Val  Ser Leu Ala 
    1250                 1255                 1260             


Asp Asn  His Ser Ile Thr Phe  Phe Asp Val His Thr  Leu Gln Gly 
    1265                 1270                 1275             


Met Asn  Ile Val Gly Ala 
    1280                 


<210>  3
<211>  2085
<212>  DNA
<213>  Escherichia coli

<400>  3
atggaaagca aacgtctgga taatgccgcg ctggcggcgg ggattagccc caattacatc       60

aatgcccacg gtaaaccgca gtcgattagc gccgaaacca aacggcgttt gcttgacgcg      120

atgcatcaac gtaccgccac gaaagtggcg gtaacgccag tcccgaatgt catggtttat      180

accagcggca aaaaaatgcc gatggtggtg gagggcagcg gcgaatatag ctggctgctg      240

accaccgaag aaggaacgca gtacaaaggc catgtaacgg ggggcaaagc gttcaatcta      300

ccgacgaagc tgccggaagg ttatcacacg ctgacactca cccaggacga ccagcgcgcg      360

cattgccggg tgattgtcgc cccgaaacgc tgttacgaac cgcaggcgtt gctgaataaa      420

caaaagctgt ggggtgcctg cgttcagctt tatacgctgc gatcggaaaa aaactggggt      480

attggggatt ttggcgatct caaagcgatg ctggtggatg tggcaaaacg tggcgggtcg      540

ttcattggcc tgaacccgat tcatgcgctc tatccggcaa atccggagag cgccagccca      600

tacagcccgt cttctcgccg ttggctgaat gtgatttata tcgacgttaa cgccgttgaa      660

gatttccatc ttagcgaaga ggctcaggcc tggtggcagt tgccgaccac gcaacagacg      720

ctgcaacagg cgcgcgatgc cgactgggtc gattactcca cggttaccgc cctaaaaatg      780

acagcattac gaatggcgtg gaaaggtttc gcgcaacgtg atgatgagca gatggccgcg      840

tttcgccagt ttgttgcaga gcagggcgac agcctgttct ggcaggcagc ctttgatgcg      900

ctacatgccc agcaagtgaa agaggacgaa atgcgctggg gctggcctgc atggccagag      960

atgtatcaga acgtggattc accagaagtg cgtcagttct gcgaagaaca tcgtgatgac     1020

gtcgattttt atctctggtt gcagtggctg gcttacagcc agtttgccgc ctgctgggag     1080

ataagccagg gctatgaaat gccgattggc ttgtatcgtg atctggcggt tggcgtagcg     1140

gaaggtgggg cggaaacctg gtgtgaccgt gaactatatt gcctgaaagc atcggttggc     1200

gcgccgccgg atatcctcgg cccgttgggg cagaactggg gattaccgcc aatggacccg     1260

catatcatca ccgcgcgtgc ctatgaaccg tttatcgagc tgttgcgtgc caatatgcaa     1320

aactgcggcg cattacgaat tgaccatgtg atgtcgatgc tgcgtttgtg gtggataccg     1380

tatggcgaga cggcagatca gggcgcgtat gttcactatc cggtggatga tctgctctcg     1440

attctggcac tcgaaagtaa acgtcatcgc tgtatggtga ttggtgaaga tctcggtacc     1500

gtaccggtag agattgtcgg taagctgcgc agcagcggtg tgtactctta caaagtgctc     1560

tatttcgaaa acgaccacga gaagacgttc cgtgcaccga aagcgtatcc ggagcagtcg     1620

atggcggttg cggcgacaca tgacctgcca acgctgcgcg gttactggga gtgcggggat     1680

ctaacgctgg gcaaaaccct ggggctgtat ccggatgaag tggtactgcg cggtctgtat     1740

caggatcgcg aactggcgaa gcaagggctg ctggatgcac tgcataaata tggttgtctg     1800

ccgaaacgtg ccgggcataa ggcatcgttg atgtcgatga cgccgacgct gaaccgtggt     1860

ttgcagcgct acattgccga cagtaacagt gctctgttag gactacagcc ggaagactgg     1920

ctggatatgg ccgaaccggt gaatattcct ggcaccagtt accagtataa aaactggcga     1980

cgcaagcttt ccgcaacgct tgagtcgatg tttgccgatg atggcgtgaa caagttgctg     2040

aaggatttgg acagacggcg cagagctgca gcgaagaaga agtag                     2085


<210>  4
<211>  2031
<212>  DNA
<213>  Escherichia coli

<400>  4
atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg       60

acttctccgg ggtttcccgc ctttagcgaa caggggacag gaacatttgt cagccacgcg      120

cagttgccca aaggtacgcg tccactaacg ctaaattttg accaacagtg ctggcagcct      180

gcggatgcga taaaactcaa tcagatgctt tccctgcaac cttgtagcaa cacgccgcct      240

caatggcgat tgttcaggga cggcgaatat acgctgcaaa tagacacccg ctccggtacg      300

ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaagcct ggtccgtgaa      360

tgcccgaaat gggatggatt accgctcaca gtggatgtca gcgccacttt cccggaagga      420

gccgccgtac gggattatta cagccagcaa attgcgatag tgaagaacgg tcaaataatg      480

ttacaacccg ctgccaccag caacggttta ctcctgctgg aacgggcaga aactgacaca      540

tccgcccctt tcgactggca taacgccacg gtttactttg tgctgacaga tcgtttcgaa      600

aacggcgatc ccagtaatga ccagagttac ggacgtcata aagacggtat ggcggaaatt      660

ggcacttttc acggcggcga tttacgcggc ctgaccaaca aactggatta cctccagcag      720

ttgggcgtta atgctttatg gataagcgcc ccatttgagc aaattcacgg ctgggtcggc      780

ggcggtacaa aaggcgattt cccgcattat gcctaccacg gttattacac acaggactgg      840

acgaatcttg atgccaatat gggcaacgaa gccgatctac ggacgctggt tgatagcgca      900

catcagcgcg gtattcgtat tctctttgat gtcgtgatga accacaccgg ctatgccacg      960

ctggcggata tgcaggagta tcagtttggc gcgttatatc tttctggtga cgaagtgaaa     1020

aaatcgctgg gtgaacgctg gagcgactgg aaacctgccg ccgggcaaac ctggcatagc     1080

tttaacgatt acattaattt cagcgacaaa acaggctggg ataaatggtg gggaaaaaac     1140

tggatcagaa cggatatcgg cgattacgac aatcctggat tcgacgatct cactatgtcg     1200

ctagcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc     1260

tataaaaaca aaatggatac ccacgccaaa gccattgacg gctatacgcc gcgcgattac     1320

ttaacccact ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat     1380

accgccaaac atgttgagtt gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg     1440

cttcgcgaat ggaaaaaagc taaccccgac aaagcattag atgacaaacc tttctggatg     1500

accggtgaag cctggggcca cggcgtgatg caaagtgact actatcgcca cggcttcgat     1560

gcgatgatca atttcgatta tcaggagcag gcggcgaaag cagtcgactg tctggcgcag     1620

atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac     1680

ctctcgtcgc atgatacccg cctgttccgt gaagggggcg acaaagcagc agagttatta     1740

ctattagcgc caggcgcggt acaaatcttt tatggtgatg aatcctcgcg tccgttcggt     1800

cctacaggtt ctgatccgct gcaaggtaca cgttcggata tgaactggca ggatgttagc     1860

ggtaaatctg ccgccagcgt cgcgcactgg cagaaaatca gccagttccg cgcccgccat     1920

cccgcaattg gcgcgggcaa acaaacgaca cttttgctga agcagggcta cggctttgtt     1980

cgtgagcatg gcgacgataa agtgctggtc gtctgggcag ggcaacagta a              2031


<210>  5
<211>  2394
<212>  DNA
<213>  Escherichia coli

<400>  5
atgtcacaac ctatttttaa cgataagcaa tttcaggaag cgctttcacg tcagtggcag       60

cgttatggct taaattctgc ggctgaaatg actcctcgcc agtggtggct agcagtgagt      120

gaagcactgg ccgaaatgct gcgtgctcag ccattcgcca agccggtggc gaatcagcga      180

catgttaact acatctcaat ggagtttttg attggtcgcc tgacgggcaa caacctgttg      240

aatctcggct ggtatcagga tgtacaggat tcgttgaagg cttatgacat caatctgacg      300

gacctgctgg aagaagagat cgacccggcg ctgggtaacg gtggtctggg acgtctggcg      360

gcgtgcttcc tcgactcaat ggcaactgtc ggtcagtctg cgacgggtta cggtctgaac      420

tatcaatatg gtttgttccg ccagtctttt gtcgatggca aacaggttga agcgccggat      480

gactggcatc gcagtaacta cccgtggttc cgccacaacg aagcactgga tgtgcaggta      540

gggattggcg gtaaagtgac gaaagacgga cgctgggagc cggagtttac cattaccggt      600

caagcgtggg atctccccgt tgtcggctat cgtaatggcg tggcgcagcc gctgcgtctg      660

tggcaggcga cgcacgcgca tccgtttgat ctgactaaat ttaacgacgg tgatttcttg      720

cgtgccgaac agcagggcat caatgcggaa aaactgacca aagttctcta tccaaacgac      780

aaccatactg ccggtaaaaa gctgcgcctg atgcagcaat acttccagtg tgcctgttcg      840

gtagcggata ttttgcgtcg ccatcatctg gcggggcgta aactgcacga actggcggat      900

tacgaagtta ttcagctgaa cgatacccac ccaactatcg cgattccaga actgctgcgc      960

gtgctgatcg atgagcacca gatgagctgg gatgacgcct gggccattac cagcaaaact     1020

ttcgcttaca ccaaccatac cctgatgcca gaagcgctgg aacgctggga tgtgaaactg     1080

gtgaaaggct tactgccgcg ccacatgcag attattaacg aaattaatac tcgctttaaa     1140

acgctggtag agaaaacctg gccgggcgat gaaaaagtgt gggccaaact ggcggtggtg     1200

cacgacaaac aagtgcatat ggcgaacctg tgtgtggttg gcggtttcgc ggtgaacggt     1260

gttgcggcgc tgcactcgga tctggtggtg aaagatctgt tcccggaata tcaccagcta     1320

tggccgaaca aattccataa cgtcaccaac ggtattaccc cacgtcgctg gatcaaacag     1380

tgcaacccgg cactggcggc tctgttggat aaatcactgc aaaaagagtg ggctaacgat     1440

ctcgatcagc tgatcaatct ggaaaaattc gctgatgatg cgaaattccg tcagcaatat     1500

cgcgagatca agcaggcgaa taaagtccgt ctggcggagt ttgtgaaagt tcgtaccggt     1560

attgagatca atccacaggc gattttcgat attcagatca aacgtttgca tgagtacaaa     1620

cgccagcacc tgaatctgct gcatattctg gcgttgtaca aagaaattcg tgaaaacccg     1680

caggctgatc gcgtaccgcg cgtcttcctc ttcggcgcga aagcggcacc gggctactac     1740

ctggcgaaga atattatctt tgcgatcaac aaagtggctg acgtgatcaa caacgatccg     1800

ctggttggcg ataagttgaa ggtggtgttc ctgccggatt attgcgtttc ggcggcggaa     1860

aaactgatcc cggcggcgga tatctccgaa caaatttcga ctgcaggtaa agaagcttcc     1920

ggtaccggca atatgaaact ggcgctcaat ggtgcgctta ctgtcggtac gctggatggg     1980

gcgaacgttg aaatcgccga gaaagtcggt gaagaaaata tctttatttt tggtcatacc     2040

gtggaacaag tgaaggcaat tctggccaaa ggctacgacc cggtgaaatg gcggaagaaa     2100

gataaggtgc tggacgcagt attgaaagag ctggaaagcg gtaaatacag cgacggcgat     2160

aagcatgcct tcgaccagat gctgcacagt atcggcaaac agggcggcga tccgtatctg     2220

gtgatggcgg atttcgcagc ctatgtagag gcacaaaagc aggtggatgt gctgtaccgc     2280

gaccaggagg cctggactcg cgcggcgatc ctcaataccg cccgctgcgg tatgtttagc     2340

tcggatcgct ctattcgcga ttatcaggct cgtatctggc aggcaaaacg ctaa           2394


<210>  6
<211>  1815
<212>  DNA
<213>  Escherichia coli

<400>  6
atgttaaatg catggcacct gccggtgccc ccatttgtta aacaaagcaa agatcaactg       60

ctcattacac tgtggctgac gggcgaagac ccaccgcagc gcattatgct gcgtacagaa      120

cacgataacg aagaaatgtc agtaccgatg cataagcagc gcagtcagcc gcagcctggc      180

gtcaccgcat ggcgtgcggc gattgatctc tccagcggac aaccccggcg gcgttacagt      240

ttcaaactgc tgtggcacga tcgccagcgt tggtttacac cgcagggctt cagccgaatg      300

ccgccggcac gactggagca gtttgccgtc gatgtaccgg atatcggccc acaatgggct      360

gcggatcaga ttttttatca gatcttccct gatcgttttg cgcgtagtct tcctcgtgaa      420

gctgaacagg atcatgtcta ttaccatcat gcagccggac aagagatcat cttgcgtgac      480

tgggatgaac cggtcacggc gcaggcgggc ggatcaacgt tctatggcgg cgatctggac      540

gggataagcg aaaaactgcc gtatctgaaa aagcttggcg tgacagcgct gtatctcaat      600

ccggtgttta aagctcccag cgtacataaa tacgataccg aggattatcg ccatgtcgat      660

ccgcagtttg gcggtgatgg ggcgttgctg cgtttgcgac acaatacgca gcagctggga      720

atgcggctgg tgctggacgg cgtgtttaac cacagtggcg attcccatgc ctggtttgac      780

aggcataatc gtggcacggg tggtgcttgt cacaaccccg aatcgccctg gcgcgactgg      840

tactcgttta gtgatgatgg cacggcgctc gactggcttg gctatgccag cttgccgaag      900

ctggattatc agtcggaaag tctggtgaat gaaatttatc gcggggaaga cagtattgtc      960

cgccactggc tgaaagcgcc gtggaatatg gacggctggc ggctggatgt ggtgcatatg     1020

ctgggggagg cgggtggggc gcgcaataat atgcagcacg ttgccgggat caccgaagcg     1080

gcgaaagaaa cccagccgga agcgtatatt gtcggcgaac attttggcga tgcacggcaa     1140

tggttacagg ccgatgtgga agatgccgcc atgaactatc gtggcttcac attcccgttg     1200

tggggatttc ttgccaatac cgatatctct tacgatccgc agcaaattga tgcccaaacc     1260

tgtatggcct ggatggataa ttaccgcgca gggctttctc atcaacaaca attacgtatg     1320

tttaatcagc tcgacagcca cgatactgcg cgatttaaaa cgctgctcgg tcgggatatt     1380

gcgcgcctgc cgctggcggt ggtctggctg ttcacctggc ctggtgtacc gtgcatttat     1440

tacggtgatg aagtaggact ggatggcaaa aacgatccgt tttgccgtaa accgttcccc     1500

tggcaggtgg aaaagcagga tacggcgtta ttcgcgctgt accagcgaat gattgcgctg     1560

cgtaagaaaa gtcaggcgct acgtcatggc ggctgtcagg tgctgtatgc ggaagataac     1620

gtggtggtat ttgtccgcgt gctgaatcag caacgtgtac tggtggcaat caaccgtggc     1680

gaggcctgtg aagtggtgct acccgcgtca ccgtttctca atgccgtgca atggcaatgc     1740

aaagaagggc atgggcaact gactgacggg attctggctt tgcctgccat ttcggctacg     1800

gtatggatga actaa                                                      1815


<210>  7
<211>  1488
<212>  DNA
<213>  Escherichia coli

<400>  7
atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg cggtaagctc       60

tggcctgaac tggccgagcg cgccgacggt tttaatgata ttggtatcaa tatggtctgg      120

ttgccgcccg cctataaagg cgcatcgggc gggtattcgg tcggctacga ctcctatgat      180

ttatttgatt taggcgagtt tgatcagaaa ggcagcatcc ctactaaata tggcgataaa      240

gcacaactgc tggccgccat tgatgctctg aaacgtaatg acattgcggt gctgttggat      300

gtggtagtca accacaaaat gggcgcggat gaaaaagaag ctattcgcgt gcagcgtgta      360

aatgctgatg accgtacgca aattgacgaa gaaatcattg agtgtgaagg ctggacgcgt      420

tacaccttcc ccgcccgtgc cgggcaatac tcgcagttta tctgggattt caaatgtttt      480

agcggtatcg accatatcga aaaccctgac gaagatggca tttttaaaat tgttaacgac      540

tacaccggcg aaggctggaa cgatcaggtt gatgatgaat taggtaattt cgattatctg      600

atgggcgaga atatcgattt tcgcaatcat gccgtgacgg aagagattaa atactgggcg      660

cgctgggtga tggaacaaac gcaatgcgac ggttttcgtc ttgatgcggt caaacatatt      720

ccagcctggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg      780

tttattgtgg cggagtactg gtcgcatgaa gttgataagc tgcaaacgta tattgatcag      840

gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga tgaaattcca tgaagcatcg      900

cgcatggggc gcgactacga catgacgcag attttcacgg gtacattagt ggaagccgat      960

cctttccacg ccgtgacgct cgttgccaat cacgacaccc aaccgttgca agccctcgaa     1020

gcgccggtcg aaccgtggtt taaaccgctg gcgtatgcct taattttgtt gcgggaaaat     1080

ggcgttcctt cggtattcta tccggacctc tacggtgcgc attacgaaga tgtcggtggt     1140

gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga gttaattctc     1200

gcccgtcagc gtttcgccca cggtgtacag acgttatttt tcgaccatcc gaactgcatt     1260

gcctttagcc gcagtggcac cgacgaattt cccggctgcg tggtggtcat gtcgaacggg     1320

gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac ctggcgtgat     1380

tttctgggga accggcaaga gagagtagtg accgacgaaa acggcgaagc aaccttcttt     1440

tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaa                  1488


<210>  8
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic construct

<400>  8
ataaaaaacg ctcggttgcc gccgggcgtt ttttat                                 36


<210>  9
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic construct

<400>  9
ggatcctgac tgcctgagct t                                                 21


<210>  10
<211>  26
<212>  PRT
<213>  Bacillus subtilis

<400>  10

Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu 
1               5                   10                  15      


Ile Phe Thr Met Ala Phe Ser Asn Met Ser 
            20                  25      


<210>  11
<211>  8616
<212>  DNA
<213>  artificial sequence

<220>
<223>  plasmid pTrex

<400>  11
aagcttaact agtacttctc gagctctgta catgtccggt cgcgacgtac gcgtatcgat       60

ggcgccagct gcaggcggcc gcctgcagcc acttgcagtc ccgtggaatt ctcacggtga      120

atgtaggcct tttgtagggt aggaattgtc actcaagcac ccccaacctc cattacgcct      180

cccccataga gttcccaatc agtgagtcat ggcactgttc tcaaatagat tggggagaag      240

ttgacttccg cccagagctg aaggtcgcac aaccgcatga tatagggtcg gcaacggcaa      300

aaaagcacgt ggctcaccga aaagcaagat gtttgcgatc taacatccag gaacctggat      360

acatccatca tcacgcacga ccactttgat ctgctggtaa actcgtattc gccctaaacc      420

gaagtgcgtg gtaaatctac acgtgggccc ctttcggtat actgcgtgtg tcttctctag      480

gtgccattct tttcccttcc tctagtgttg aattgtttgt gttggagtcc gagctgtaac      540

tacctctgaa tctctggaga atggtggact aacgactacc gtgcacctgc atcatgtata      600

taatagtgat cctgagaagg ggggtttgga gcaatgtggg actttgatgg tcatcaaaca      660

aagaacgaag acgcctcttt tgcaaagttt tgtttcggct acggtgaaga actggatact      720

tgttgtgtct tctgtgtatt tttgtggcaa caagaggcca gagacaatct attcaaacac      780

caagcttgct cttttgagct acaagaacct gtggggtata tatctagagt tgtgaagtcg      840

gtaatcccgc tgtatagtaa tacgagtcgc atctaaatac tccgaagctg ctgcgaaccc      900

ggagaatcga gatgtgctgg aaagcttcta gcgagcggct aaattagcat gaaaggctat      960

gagaaattct ggagacggct tgttgaatca tggcgttcca ttcttcgaca agcaaagcgt     1020

tccgtcgcag tagcaggcac tcattcccga aaaaactcgg agattcctaa gtagcgatgg     1080

aaccggaata atataatagg caatacattg agttgcctcg acggttgcaa tgcaggggta     1140

ctgagcttgg acataactgt tccgtacccc acctcttctc aacctttggc gtttccctga     1200

ttcagcgtac ccgtacaagt cgtaatcact attaacccag actgaccgga cgtgttttgc     1260

ccttcatttg gagaaataat gtcattgcga tgtgtaattt gcctgcttga ccgactgggg     1320

ctgttcgaag cccgaatgta ggattgttat ccgaactctg ctcgtagagg catgttgtga     1380

atctgtgtcg ggcaggacac gcctcgaagg ttcacggcaa gggaaaccac cgatagcagt     1440

gtctagtagc aacctgtaaa gccgcaatgc agcatcactg gaaaatacaa accaatggct     1500

aaaagtacat aagttaatgc ctaaagaagt catataccag cggctaataa ttgtacaatc     1560

aagtggctaa acgtaccgta atttgccaac ggcttgtggg gttgcagaag caacggcaaa     1620

gccccacttc cccacgtttg tttcttcact cagtccaatc tcagctggtg atcccccaat     1680

tgggtcgctt gtttgttccg gtgaagtgaa agaagacaga ggtaagaatg tctgactcgg     1740

agcgttttgc atacaaccaa gggcagtgat ggaagacagt gaaatgttga cattcaagga     1800

gtatttagcc agggatgctt gagtgtatcg tgtaaggagg tttgtctgcc gatacgacga     1860

atactgtata gtcacttctg atgaagtggt ccatattgaa atgtaagtcg gcactgaaca     1920

ggcaaaagat tgagttgaaa ctgcctaaga tctcgggccc tcgggccttc ggcctttggg     1980

tgtacatgtt tgtgctccgg gcaaatgcaa agtgtggtag gatcgaacac actgctgcct     2040

ttaccaagca gctgagggta tgtgataggc aaatgttcag gggccactgc atggtttcga     2100

atagaaagag aagcttagcc aagaacaata gccgataaag atagcctcat taaacggaat     2160

gagctagtag gcaaagtcag cgaatgtgta tatataaagg ttcgaggtcc gtgcctccct     2220

catgctctcc ccatctactc atcaactcag atcctccagg agacttgtac accatctttt     2280

gaggcacaga aacccaatag tcaaccgcgg actgcgcatc atgtatcgga agttggccgt     2340

catctcggcc ttcttggcca cacctcgtgc tagactaggc gcgccgcgcg ccagctccgt     2400

gcgaaagcct gacgcaccgg tagattcttg gtgagcccgt atcatgacgg cggcgggagc     2460

tacatggccc cgggtgattt attttttttg tatctacttc tgaccctttt caaatatacg     2520

gtcaactcat ctttcactgg agatgcggcc tgcttggtat tgcgatgttg tcagcttggc     2580

aaattgtggc tttcgaaaac acaaaacgat tccttagtag ccatgcattt taagataacg     2640

gaatagaaga aagaggaaat taaaaaaaaa aaaaaaacaa acatcccgtt cataacccgt     2700

agaatcgccg ctcttcgtgt atcccagtac cagtttattt tgaatagctc gcccgctgga     2760

gagcatcctg aatgcaagta acaaccgtag aggctgacac ggcaggtgtt gctagggagc     2820

gtcgtgttct acaaggccag acgtcttcgc ggttgatata tatgtatgtt tgactgcagg     2880

ctgctcagcg acgacagtca agttcgccct cgctgcttgt gcaataatcg cagtggggaa     2940

gccacaccgt gactcccatc tttcagtaaa gctctgttgg tgtttatcag caatacacgt     3000

aatttaaact cgttagcatg gggctgatag cttaattacc gtttaccagt gccatggttc     3060

tgcagctttc cttggcccgt aaaattcggc gaagccagcc aatcaccagc taggcaccag     3120

ctaaacccta taattagtct cttatcaaca ccatccgctc ccccgggatc aatgaggaga     3180

atgaggggga tgcggggcta aagaagccta cataaccctc atgccaactc ccagtttaca     3240

ctcgtcgagc caacatcctg actataagct aacacagaat gcctcaatcc tgggaagaac     3300

tggccgctga taagcgcgcc cgcctcgcaa aaaccatccc tgatgaatgg aaagtccaga     3360

cgctgcctgc ggaagacagc gttattgatt tcccaaagaa atcggggatc ctttcagagg     3420

ccgaactgaa gatcacagag gcctccgctg cagatcttgt gtccaagctg gcggccggag     3480

agttgacctc ggtggaagtt acgctagcat tctgtaaacg ggcagcaatc gcccagcagt     3540

tagtagggtc ccctctacct ctcagggaga tgtaacaacg ccaccttatg ggactatcaa     3600

gctgacgctg gcttctgtgc agacaaactg cgcccacgag ttcttccctg acgccgctct     3660

cgcgcaggca agggaactcg atgaatacta cgcaaagcac aagagacccg ttggtccact     3720

ccatggcctc cccatctctc tcaaagacca gcttcgagtc aaggtacacc gttgccccta     3780

agtcgttaga tgtccctttt tgtcagctaa catatgccac cagggctacg aaacatcaat     3840

gggctacatc tcatggctaa acaagtacga cgaaggggac tcggttctga caaccatgct     3900

ccgcaaagcc ggtgccgtct tctacgtcaa gacctctgtc ccgcagaccc tgatggtctg     3960

cgagacagtc aacaacatca tcgggcgcac cgtcaaccca cgcaacaaga actggtcgtg     4020

cggcggcagt tctggtggtg agggtgcgat cgttgggatt cgtggtggcg tcatcggtgt     4080

aggaacggat atcggtggct cgattcgagt gccggccgcg ttcaacttcc tgtacggtct     4140

aaggccgagt catgggcggc tgccgtatgc aaagatggcg aacagcatgg agggtcagga     4200

gacggtgcac agcgttgtcg ggccgattac gcactctgtt gagggtgagt ccttcgcctc     4260

ttccttcttt tcctgctcta taccaggcct ccactgtcct cctttcttgc tttttatact     4320

atatacgaga ccggcagtca ctgatgaagt atgttagacc tccgcctctt caccaaatcc     4380

gtcctcggtc aggagccatg gaaatacgac tccaaggtca tccccatgcc ctggcgccag     4440

tccgagtcgg acattattgc ctccaagatc aagaacggcg ggctcaatat cggctactac     4500

aacttcgacg gcaatgtcct tccacaccct cctatcctgc gcggcgtgga aaccaccgtc     4560

gccgcactcg ccaaagccgg tcacaccgtg accccgtgga cgccatacaa gcacgatttc     4620

ggccacgatc tcatctccca tatctacgcg gctgacggca gcgccgacgt aatgcgcgat     4680

atcagtgcat ccggcgagcc ggcgattcca aatatcaaag acctactgaa cccgaacatc     4740

aaagctgtta acatgaacga gctctgggac acgcatctcc agaagtggaa ttaccagatg     4800

gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg ggaaggaact ggacgccatc     4860

atcgcgccga ttacgcctac cgctgcggta cggcatgacc agttccggta ctatgggtat     4920

gcctctgtga tcaacctgct ggatttcacg agcgtggttg ttccggttac ctttgcggat     4980

aagaacatcg ataagaagaa tgagagtttc aaggcggtta gtgagcttga tgccctcgtg     5040

caggaagagt atgatccgga ggcgtaccat ggggcaccgg ttgcagtgca ggttatcgga     5100

cggagactca gtgaagagag gacgttggcg attgcagagg aagtggggaa gttgctggga     5160

aatgtggtga ctccatagct aataagtgtc agatagcaat ttgcacaaga aatcaatacc     5220

agcaactgta aataagcgct gaagtgacca tgccatgcta cgaaagagca gaaaaaaacc     5280

tgccgtagaa ccgaagagat atgacacgct tccatctctc aaaggaagaa tcccttcagg     5340

gttgcgtttc cagtctagac acgtataacg gcacaagtgt ctctcaccaa atgggttata     5400

tctcaaatgt gatctaagga tggaaagccc agaatatcga tcgcgcgcag atccatatat     5460

agggcccggg ttataattac ctcaggtcga cgtcccatgg ccattcgaat tcgtaatcat     5520

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag     5580

ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg     5640

cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa     5700

tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca     5760

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     5820

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     5880

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     5940

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     6000

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     6060

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     6120

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     6180

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     6240

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     6300

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     6360

gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     6420

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc     6480

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt     6540

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa     6600

ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat     6660

atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga     6720

tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac     6780

gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg     6840

ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg     6900

caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt     6960

cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct     7020

cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat     7080

cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta     7140

agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca     7200

tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat     7260

agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac     7320

atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa     7380

ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt     7440

cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg     7500

caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat     7560

attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt     7620

agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct     7680

aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc     7740

gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg     7800

tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg     7860

gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag     7920

tgcaccataa aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa     7980

tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat     8040

agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg     8100

tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac     8160

catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta     8220

aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag     8280

ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg     8340

taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtactat ggttgctttg     8400

acgtatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca     8460

ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     8520

acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt     8580

ttcccagtca cgacgttgta aaacgacggc cagtgc                               8616


<210>  12
<211>  636
<212>  PRT
<213>  Neisseria polysaccharea

<400>  12

Met Leu Thr Pro Thr Gln Gln Val Gly Leu Ile Leu Gln Tyr Leu Lys 
1               5                   10                  15      


Thr Arg Ile Leu Asp Ile Tyr Thr Pro Glu Gln Arg Ala Gly Ile Glu 
            20                  25                  30          


Lys Ser Glu Asp Trp Arg Gln Phe Ser Arg Arg Met Asp Thr His Phe 
        35                  40                  45              


Pro Lys Leu Met Asn Glu Leu Asp Ser Val Tyr Gly Asn Asn Glu Ala 
    50                  55                  60                  


Leu Leu Pro Met Leu Glu Met Leu Leu Ala Gln Ala Trp Gln Ser Tyr 
65                  70                  75                  80  


Ser Gln Arg Asn Ser Ser Leu Lys Asp Ile Asp Ile Ala Arg Glu Asn 
                85                  90                  95      


Asn Pro Asp Trp Ile Leu Ser Asn Lys Gln Val Gly Gly Val Cys Tyr 
            100                 105                 110         


Val Asp Leu Phe Ala Gly Asp Leu Lys Gly Leu Lys Asp Lys Ile Pro 
        115                 120                 125             


Tyr Phe Gln Glu Leu Gly Leu Thr Tyr Leu His Leu Met Pro Leu Phe 
    130                 135                 140                 


Lys Cys Pro Glu Gly Lys Ser Asp Gly Gly Tyr Ala Val Ser Ser Tyr 
145                 150                 155                 160 


Arg Asp Val Asn Pro Ala Leu Gly Thr Ile Gly Asp Leu Arg Glu Val 
                165                 170                 175     


Ile Ala Ala Leu His Glu Ala Gly Ile Ser Ala Val Val Asp Phe Ile 
            180                 185                 190         


Phe Asn His Thr Ser Asn Glu His Glu Trp Ala Gln Arg Cys Ala Ala 
        195                 200                 205             


Gly Asp Pro Leu Phe Asp Asn Phe Tyr Tyr Ile Phe Pro Asp Arg Arg 
    210                 215                 220                 


Met Pro Asp Gln Tyr Asp Arg Thr Leu Arg Glu Ile Phe Pro Asp Gln 
225                 230                 235                 240 


His Pro Gly Gly Phe Ser Gln Leu Glu Asp Gly Arg Trp Val Trp Thr 
                245                 250                 255     


Thr Phe Asn Ser Phe Gln Trp Asp Leu Asn Tyr Ser Asn Pro Trp Val 
            260                 265                 270         


Phe Arg Ala Met Ala Gly Glu Met Leu Phe Leu Ala Asn Leu Gly Val 
        275                 280                 285             


Asp Ile Leu Arg Met Asp Ala Val Ala Phe Ile Trp Lys Gln Met Gly 
    290                 295                 300                 


Thr Ser Cys Glu Asn Leu Pro Gln Ala His Ala Leu Ile Arg Ala Phe 
305                 310                 315                 320 


Asn Ala Val Met Arg Ile Ala Ala Pro Ala Val Phe Phe Lys Ser Glu 
                325                 330                 335     


Ala Ile Val His Pro Asp Gln Val Val Gln Tyr Ile Gly Gln Asp Glu 
            340                 345                 350         


Cys Gln Ile Gly Tyr Asn Pro Leu Gln Met Ala Leu Leu Trp Asn Thr 
        355                 360                 365             


Leu Ala Thr Arg Glu Val Asn Leu Leu His Gln Ala Leu Thr Tyr Arg 
    370                 375                 380                 


His Asn Leu Pro Glu His Thr Ala Trp Val Asn Tyr Val Arg Ser His 
385                 390                 395                 400 


Asp Asp Ile Gly Trp Thr Phe Ala Asp Glu Asp Ala Ala Tyr Leu Gly 
                405                 410                 415     


Ile Ser Gly Tyr Asp His Arg Gln Phe Leu Asn Arg Phe Phe Val Asn 
            420                 425                 430         


Arg Phe Asp Gly Ser Phe Ala Arg Gly Val Pro Phe Gln Tyr Asn Pro 
        435                 440                 445             


Ser Thr Gly Asp Cys Arg Val Ser Gly Thr Ala Ala Ala Leu Val Gly 
    450                 455                 460                 


Leu Ala Gln Asp Asp Pro His Ala Val Asp Arg Ile Lys Leu Leu Tyr 
465                 470                 475                 480 


Ser Ile Ala Leu Ser Thr Gly Gly Leu Pro Leu Ile Tyr Leu Gly Asp 
                485                 490                 495     


Glu Val Gly Thr Leu Asn Asp Asp Asp Trp Ser Gln Asp Ser Asn Lys 
            500                 505                 510         


Ser Asp Asp Ser Arg Trp Ala His Arg Pro Arg Tyr Asn Glu Ala Leu 
        515                 520                 525             


Tyr Ala Gln Arg Asn Asp Pro Ser Thr Ala Ala Gly Gln Ile Tyr Gln 
    530                 535                 540                 


Gly Leu Arg His Met Ile Ala Val Arg Gln Ser Asn Pro Arg Phe Asp 
545                 550                 555                 560 


Gly Gly Arg Leu Val Thr Phe Asn Thr Asn Asn Lys His Ile Ile Gly 
                565                 570                 575     


Tyr Ile Arg Asn Asn Ala Leu Leu Ala Phe Gly Asn Phe Ser Glu Tyr 
            580                 585                 590         


Pro Gln Thr Val Thr Ala His Thr Leu Gln Ala Met Pro Phe Lys Ala 
        595                 600                 605             


His Asp Leu Ile Gly Gly Lys Thr Val Ser Leu Asn Gln Asp Leu Thr 
    610                 615                 620                 


Leu Gln Pro Tyr Gln Val Met Trp Leu Glu Ile Ala 
625                 630                 635     


