                         SEQUENCE LISTING

<110>  Tyton BioSciences, LLC
 
<120>  TRANSGENIC TOBACCO PLANTS FOR ENHANCED BIOETHANOL PRODUCTION

<130>  448/2 PCT

<150>  62004181
<151>  2014-05-28

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  517
<212>  DNA
<213>  Nicotiana tabacum

<400>  1
tctagaaacc atgaagaatt tgattttcct aacgatgttt ctgactatat tactacaaac       60

aaacgccaat aatctagtag aaactacatg caaaaacaca ccaaattacc aactttgtct      120

gaaaactctg ctttcggaca aacgaagtgc aacaggggat atcacaacgt tggcactaat      180

tatggtcgat gcaataaaag ctaaagctaa tcaggctgca gtgacaattt cgaaactccg      240

gcattcgaat ccccctgcag cttggaaagg tcctttgaaa aactgtgcct tttcatataa      300

ggtaatttta acagcaagtt tgcctgaagc aattgaagca ttgacaaaag gagatccaaa      360

atttgctgaa gatggaatgg taggttcatc tggagatgca caagaatgtg aggagtattt      420

caagggtagt aaatcaccat tttctgcatt aaatatagca gttcatgaac tttctgatgt      480

tgggagagct attgtcagaa atttattgtg agagctc                               517


<210>  2
<211>  166
<212>  PRT
<213>  Nicotiana tabacum

<400>  2

Met Lys Asn Leu Ile Phe Leu Thr Met Phe Leu Thr Ile Leu Leu Gln 
1               5                   10                  15      


Thr Asn Ala Asn Asn Leu Val Glu Thr Thr Cys Lys Asn Thr Pro Asn 
            20                  25                  30          


Tyr Gln Leu Cys Leu Lys Thr Leu Leu Ser Asp Lys Arg Ser Ala Thr 
        35                  40                  45              


Gly Asp Ile Thr Thr Leu Ala Leu Ile Met Val Asp Ala Ile Lys Ala 
    50                  55                  60                  


Lys Ala Asn Gln Ala Ala Val Thr Ile Ser Lys Leu Arg His Ser Asn 
65                  70                  75                  80  


Pro Pro Ala Ala Trp Lys Gly Pro Leu Lys Asn Cys Ala Phe Ser Tyr 
                85                  90                  95      


Lys Val Ile Leu Thr Ala Ser Leu Pro Glu Ala Ile Glu Ala Leu Thr 
            100                 105                 110         


Lys Gly Asp Pro Lys Phe Ala Glu Asp Gly Met Val Gly Ser Ser Gly 
        115                 120                 125             


Asp Ala Gln Glu Cys Glu Glu Tyr Phe Lys Gly Ser Lys Ser Pro Phe 
    130                 135                 140                 


Ser Ala Leu Asn Ile Ala Val His Glu Leu Ser Asp Val Gly Arg Ala 
145                 150                 155                 160 


Ile Val Arg Asn Leu Leu 
                165     


<210>  3
<211>  535
<212>  DNA
<213>  Nicotiana tabacum

<400>  3
tctagaaacc atgagaaact tattccccat atttatgtta atcaccaatc tagcattcaa       60

cgacaacaac aacagtaata atatcatcaa cacgacctgc agagccacca caaactaccc      120

cttgtgcctc accaccctcc actctgatcc ccgtacctcc gaggccgagg gggcggacct      180

caccaccctc ggcctcgtca tggtagatgc ggtaaaatta aagtccatcg aaataatgaa      240

aagtataaaa aaactcgaaa aatcgaaccc cgagttgaga ctacctctta gccaatgtta      300

catagtgtat tatgctgttc tacatgctga tgtaactgtt gctgttgaag ctttaaaaag      360

aggagtccct aaatttgctg aaaatggaat ggttgatgtt gctgtagaag cagaaacttg      420

tgagtttagt tttaagtata atggattggt ttctccagtt tctgatatga ataaggagat      480

tattgaactg tcttctgtgg ctaaatctat tattagaatg ctattatgag agctc           535


<210>  4
<211>  172
<212>  PRT
<213>  Nicotiana tabacum

<400>  4

Met Arg Asn Leu Phe Pro Ile Phe Met Leu Ile Thr Asn Leu Ala Phe 
1               5                   10                  15      


Asn Asp Asn Asn Asn Ser Asn Asn Ile Ile Asn Thr Thr Cys Arg Ala 
            20                  25                  30          


Thr Thr Asn Tyr Pro Leu Cys Leu Thr Thr Leu His Ser Asp Pro Arg 
        35                  40                  45              


Thr Ser Glu Ala Glu Gly Ala Asp Leu Thr Thr Leu Gly Leu Val Met 
    50                  55                  60                  


Val Asp Ala Val Lys Leu Lys Ser Ile Glu Ile Met Lys Ser Ile Lys 
65                  70                  75                  80  


Lys Leu Glu Lys Ser Asn Pro Glu Leu Arg Leu Pro Leu Ser Gln Cys 
                85                  90                  95      


Tyr Ile Val Tyr Tyr Ala Val Leu His Ala Asp Val Thr Val Ala Val 
            100                 105                 110         


Glu Ala Leu Lys Arg Gly Val Pro Lys Phe Ala Glu Asn Gly Met Val 
        115                 120                 125             


Asp Val Ala Val Glu Ala Glu Thr Cys Glu Phe Ser Phe Lys Tyr Asn 
    130                 135                 140                 


Gly Leu Val Ser Pro Val Ser Asp Met Asn Lys Glu Ile Ile Glu Leu 
145                 150                 155                 160 


Ser Ser Val Ala Lys Ser Ile Ile Arg Met Leu Leu 
                165                 170         


<210>  5
<211>  953
<212>  DNA
<213>  Arabidopsis thaliana

<400>  5
aagcttgcaa aatagcacac catttatgtt tatattttca aattatttat tacatttcaa       60

tatttcataa gtgtgatttt tttttttttt gtcaatttca taagtgtgat ttgtcatttg      120

tattaaacaa ttgtatcgcg cagtacaaat aaacagtggg agaggtgaaa atgcagttat      180

aaaactgtcc aataatttac taacacattt aaatatctaa aaagagtgtt tcaaaaaaaa      240

ttcttttgaa ataagaaaag tgatagatat ttttacgctt tcgtctgaaa ataaaacaat      300

aatagtttat tagaaaaatg ttatcaccga aaattattct agtgccactt gctcggatcg      360

aaattcgaaa gttatattct ttctctttac ctaatataaa aatcacaaga aaaatcaatc      420

cgaatatatc tatcaacata gtatatgccc ttacatattg tttctgactt ttctctatcc      480

gaatttctcg cttcatggtt tttttttaac atattctcat ttaattttca ttactattat      540

ataactaaaa gatggaaata aaataaagtg tctttgagaa tcgaacgtcc atatcagtaa      600

gatagtttgt gtgaaggtaa aatctaaaag atttaagttc caaaaacaga aaataatata      660

ttacgctaga aaagaagaaa ataattaaat acaaaacaga aaaaaataat atacgacaga      720

cacgtgtcac gaagataccc tacgctatag acacagctct gttttctctt ttctatgcct      780

caaggctctc ttaacttcac tgtctcctct tcggataatc ctatccttct cttcctataa      840

atacctctcc actcttcctc ttcctccacc actacaacca ccgcaacaac caccaaaaac      900

cctctcaaag aaatttcttt tttttcttac tttcttggtt tgtcaaatct aga             953


<210>  6
<211>  882
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rolC promoter

<400>  6
aagcttaaag ttggcccgct attggatttc gcgaaagcgg cattggcaaa cgtgaagatt       60

gctgcattca agatactttt tctattttct ggttaagatg taaagtattg ccacaatcat      120

attaattact aacattgtat atgtaatata gtgcggagat tatctatgcc aaaatgatgt      180

attaataata gcaataataa tatgtgttaa tctttttcaa tcgggaatac gtttaagcga      240

ttatcgtgtt gaataaatta ttccaaaagg aaatacatgg ttttggagaa cctgctatag      300

atatatgcca aatttacact agtttagtgg gtgcaaaact attatctctg tttctgagtt      360

taataaaaaa taaataagca gggcgaatag cagttagcct aagaaggaat ggtggccatg      420

tacgtgcttt taagagaccc tataataaat tgccagctgt gttgctttgg tgccgacagg      480

cctaacgtgg ggtttagctt gacaaagtag cgcctttccg cagcataaat aaaggtaggc      540

gggtgcgtcc cattattaaa ggaaaaagca aaagctgaga ttccatagac cacaaaccac      600

cattattgga ggacagaacc tattccctca cgtgggtcgc tagctttaaa cctaataagt      660

aaaaacaatt aaaagcaggc aggtgtccct tctatattcg cacaacgagg cgacgtggag      720

catcgacagc cgcatccatt aattaataaa tttgtggacc tatacctaac tcaaatattt      780

ttattatttg ctccaatacg ctaacagctc tggattataa atagtttgaa tgcttcgagt      840

tatgggtaca agcaacctgt ttcctacttt gttaactcta ga                         882


<210>  7
<211>  1004
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rbcS promoter

<400>  7
aagcttagac aaacacccct tgttatacaa agaatttcgc tttacaaaat caaattcgag       60

aaaataatat atgcactaaa taagatcatt cggatctaat ctaaccaatt acgatacgct      120

ttgggtacac ttgatttttg tttcagtggt tacatatatc ttgttttata tgctatcttt      180

aaggatctgc acaaagatta tttgttgatg ttcttgatgg ggctcagaag atttgatatg      240

atacactcta atctttagga gataccagcc aggattatat tcagtaagac aatcaaattt      300

tacgtgttca aactcgttat cttttcattc aaaggatgag ccagaatctt tatagaatga      360

ttgcaatcga gaatatgttc ggccgatatg cctttgttgg cttcaatatt ctacatatca      420

cacaagaatc gaccgtattg taccctcttt ccataaagga aaacacaata tgcagatgct      480

tttttcccac atgcagtaac atataggtat tcaaaaatgg ctaaaagaag ttggataaca      540

aattgacaac tatttccatt tctgttatat aaatttcaca acacacaaaa gcccgtaatc      600

aagagtctgc ccatgtacga aataacttct attatttggt attgggccta agcccagctc      660

agagtacgtg ggggtaccac atataggaag gtaacaaaat actgcaagat agccccataa      720

cgtaccagcc tctccttacc acgaagagat aagatataag acccaccctg ccacgtgtca      780

catcgtcatg gtggttaatg ataagggatt acatccttct atgtttgtgg acatgatgca      840

tgtaatgtca tgagccacaa gatccaatgg ccacaggaac gtaagaatgt agatagattt      900

gattttgtcc gttagatagc aaacaacatt ataaaaggtg tgtatcaata ggaactaatt      960

cactcattgg attcatagaa gtccattcct cctaagtatc taga                      1004


<210>  8
<211>  870
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CaMV 35S promoter

<400>  8
aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc       60

cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata      120

atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga      180

ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag      240

tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta      300

aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa      360

cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca      420

agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca      480

aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg      540

gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa      600

aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg      660

cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag      720

aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa      780

gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat      840

ttcatttgga gagaacacgg gggactctag                                       870


<210>  9
<211>  558
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial phosphinothricin resistance gene

<400>  9
tcgacatgtc tccggagagg agaccagttg agattaggcc agctacagca gctgatatgg       60

ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc tacagtgaac tttaggacag      120

agccacaaac accacaagag tggattgatg atctagagag gttgcaagat agataccctt      180

ggttggttgc tgaggttgag ggtgttgtgg ctggtattgc ttacgctggg ccctggaagg      240

ctaggaacgc ttacgattgg acagttgaga gtactgttta cgtgtcacat aggcatcaaa      300

ggttgggcct aggatccaca ttgtacacac atttgcttaa gtctatggag gcgcaaggtt      360

ttaagtctgt ggttgctgtt ataggccttc caaacgatcc atctgttagg ttgcatgagg      420

ctttgggata cacagcccgg ggtacattgc gcgcagctgg atacaagcat ggtggatggc      480

atgatgttgg tttttggcaa agggattttg agttgccagc tcctccaagg ccagttaggc      540

cagttaccca gatctgag                                                    558


<210>  10
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AscI-PAT.F primer

<400>  10
gcttggcgcg cccatggagt caaagattca                                        30


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nhel-PAT.R primer

<400>  11
gcttgctagc gagctcggta cccactgga                                         29


<210>  12
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enoylpyrovyl-shikimate 3 phosphate synthase (EPSPS) gene

<400>  12

Met Glu Ser Leu Thr Leu Gln Pro Ile Ala His Val Asp Gly Thr Ile 
1               5                   10                  15      


Asn Leu Pro Gly Ser Lys Thr Val Ser Asn Arg Ala Leu Leu Leu Ala 
            20                  25                  30          


Ala Leu Ala His Gly Lys Thr Val Leu Thr Asn Leu Leu Asp Ser Asp 
        35                  40                  45              


Asp Val Arg His Met Leu Asn Ala Leu Thr Ala Leu Gly Val Ser Tyr 
    50                  55                  60                  


Thr Leu Ser Ala Asp Arg Thr Arg Cys Glu Ile Ile Gly Asn Gly Gly 
65                  70                  75                  80  


Pro Leu His Ala Glu Gly Ala Leu Glu Leu Phe Leu Gly Asn Ala Gly 
                85                  90                  95      


Thr Ala Met Arg Pro Leu Ala Ala Ala Leu Cys Leu Gly Ser Asn Asp 
            100                 105                 110         


Ile Val Leu Thr Gly Glu Pro Arg Met Lys Glu Arg Pro Ile Gly His 
        115                 120                 125             


Leu Val Asp Ala Leu Arg Leu Gly Gly Ala Lys Ile Thr Tyr Leu Glu 
    130                 135                 140                 


Gln Glu Asn Tyr Pro Pro Leu Arg Leu Gln Gly Gly Phe Thr Gly Gly 
145                 150                 155                 160 


Asn Val Asp Val Asp Gly Ser Val Ser Ser Gln Phe Leu Thr Ala Leu 
                165                 170                 175     


Leu Met Thr Ala Pro Leu Ala Pro Glu Asp Thr Val Ile Arg Ile Lys 
            180                 185                 190         


Gly Asp Leu Val Ser Lys Pro Tyr Ile Asp Ile Thr Leu Asn Leu Met 
        195                 200                 205             


Lys Thr Phe Gly Val Glu Ile Glu Asn Gln His Tyr Gln Gln Phe Val 
    210                 215                 220                 


Val Lys Gly Gly Gln Ser Tyr Gln Ser Pro Gly Thr Tyr Leu Val Glu 
225                 230                 235                 240 


Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Ala Ala Ala Ile Lys 
                245                 250                 255     


Gly Gly Thr Val Lys Val Thr Gly Ile Gly Arg Asn Ser Met Gln Gly 
            260                 265                 270         


Asp Ile Arg Phe Ala Asp Val Leu Glu Lys Met Gly Ala Thr Ile Cys 
        275                 280                 285             


Trp Gly Asp Asp Tyr Ile Ser Cys Thr Arg Gly Glu Leu Asn Ala Ile 
    290                 295                 300                 


His Met Asp Met Asn His Ile Pro Asp Ala Ala Met Thr Ile Ala Thr 
305                 310                 315                 320 


Ala Ala Leu Phe Ala Lys Gly Thr Thr Arg Leu Arg Asn Ile Tyr Asn 
                325                 330                 335     


Trp Arg Val Lys Glu Thr Asp Arg Leu Phe Ala Met Ala Thr Glu Leu 
            340                 345                 350         


Arg Lys Val Gly Ala Glu Val Glu Glu Gly His Asp Tyr Ile Arg Ile 
        355                 360                 365             


Thr Pro Pro Glu Lys Leu Asn Phe Ala Glu Ile Ala Thr Tyr Asn Asp 
    370                 375                 380                 


His Arg Met Ala Met Cys Phe Ser Leu Val Ala Leu Ser Asp Thr Pro 
385                 390                 395                 400 


Val Thr Ile Leu Asp Pro Lys Cys Thr Ala Lys Thr Phe Pro Asp Tyr 
                405                 410                 415     


Phe Glu Gln Leu Ala Arg Ile Ser Gln Ala Ala 
            420                 425         


<210>  13
<211>  55
<212>  PRT
<213>  Brassica napus

<400>  13

Met Ala Tyr Ser Met Leu Ser Ser Ala Thr Val Val Ser Ser Pro Ala 
1               5                   10                  15      


Gln Ala Ala Met Val Ala Pro Phe Thr Gly Leu Lys Ser Ser Ala Ala 
            20                  25                  30          


Phe Pro Val Thr Arg Lys Thr Asp Thr Asp Ile Thr Ser Met Ala Ser 
        35                  40                  45              


Asn Gly Gly Arg Val Asn Ser 
    50                  55  


<210>  14
<211>  1838
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GLY-R gene

<400>  14
gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc ccctcggtat       60

ccaattagag tctcatattc actctcaatc caaataatct gcaccggatc tggatcgttt      120

cgcatggctt attctatgct ttcttctgct actgttgttt cttctccagc tcaagctgct      180

atggttgctc catttactgg acttaagtct tctgctgctt ttccagttac tagaaagact      240

gatactgata ttacttctat ggcttctaat ggaggaagag ttaattctat ggaatctctt      300

actcttcaac caattgctca tgttgatgga actattaatc ttccaggatc taagactgtt      360

tctaataggg cacttcttct tgctgctctt gctcatggaa agactgttct tactaatctt      420

cttgattctg atgatgttag acacatgctt aatgctctta ctgctcttgg agtttcttat      480

actctttctg ctgatagaac tagatgtgaa attattggaa atggaggacc acttcatgct      540

gaaggtgcac ttgaactttt tcttggaaat gctggaactg ctatgagacc acttgctgct      600

gctctttgtc ttggatctaa tgatattgtt cttactggag aaccaagaat gaaggaaaga      660

ccaattggac atcttgttga tgctcttaga cttggaggag ctaagattac ttatcttgaa      720

caagaaaatt atccaccact tagacttcaa ggaggattta ctggaggaaa tgttgatgtt      780

gatggatctg tttcttctca atttcttact gctcttctta tgactgctcc acttgctcca      840

gaagatactg ttattagaat taagggtgat ttagtttcta agccatatat tgatattact      900

cttaatctta tgaagacttt tggagttgaa attgaaaatc aacattatca acaatttgtt      960

gttaagggag gacaatctta tcaatctcca ggaacttatc ttgttgaagg agatgcttct     1020

tctgcttctt attttcttgc tgctgctgct attaagggag gaactgttaa ggttactgga     1080

attggaagaa attctatgca aggagatatt agatttgctg atgttcttga aaagatggga     1140

gctactattt gttggggaga tgattatatt tcttgtacta gaggagaact taatgctatt     1200

cacatggata tgaatcatat tccagatgct gctatgacta ttgctactgc tgctcttttt     1260

gctaagggaa ctactagact tagaaacatc tataattgga gagttaagga aactgataga     1320

ctttttgcta tggctactga acttagaaag gttggagctg aagttgaaga aggacatgat     1380

tatattagaa ttactccacc agaaaagttg aattttgctg aaattgctac ttacaacgac     1440

catagaatgg ctatgtgttt ttctcttgtt gctctttctg atactccagt tactattctt     1500

gatccaaagt gtactgctaa gacttttcca gattattttg aacaacttgc tagaatttct     1560

caagctgctt gatccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc     1620

ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa     1680

taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc     1740

aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat     1800

cgcgcgcggt gtcatctatg ttactagatc ggcgcgcc                             1838


